AT5G46370

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009345
Physical Location & Seq
Reverse (-)
18809496 .. 18811870
2375 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46370.1

Sequence Viewer

Length: 1332 bp
ATGGCTAACGACGGTAACGGCGATAACAACGATGACCCATTACGCCAATACTTGATGAACCCAAGAATCAACCCACCACCACCGTCTCTGTTAACATTACCGGAGAACAATGACGTCACAATTCCGATGCCAATAACTCCATTGGAACTTAAAAACCGATTAATCTTCGGTTCATTCGTTCGTTCTAGAAAAGAATCATCTCTTCCCATCGATGCTCTCTCTCAAAACCCTTCTACGTCATCCTCCGCCACAACCTCATTCTCCGATTCCACAGACCTCCTTCTCCCACTTACGGAGCCTAATAAACCGGTTCGGAAATCTAAACCGACCATCAATTTCCACCGGTCTAAAACAGCTCCTGCTATGGCTGCAATCAACAATATATCTCATCCCAATGATCCCAAAACAGATCAGCAATCTGATTCGAAAACTATTGTAAACCAAGCTGTTGCTTTACTTGTTGTGTATCTATCCTTAGGTGTGCTTATCTATTGGTTGAATCGAGATAGCTACAATGTGAAGCAGACTCATCCTGTTGTTGATGCTTTGTACTTTTGTATTGTTACGATGTGTACGATTGGGTATGGAGATATAACGCCGGATAGCGTGGTGACCAAGCTGTTTTCGATATTTTTTGTGCTTGTTGGGTTTGGTTTTATGGATATATTGCTTAGTGGGATGGTTACTTATGTTCTTGATCTTCAAGAAAACTATATGTTGGAAACTGCGAGGAATGAAAGCTTGAACTTGAATGATCGGGACAAAGTGAGATCGTATATAATCGATGTCAAGAAAGGGAGGATGAGGATTAGGTTGAAAGTAGGGTTGGCATTAGGTGTTGTGGTGTTATGCCTTGGATTTGGAGTTTTGATTATGCATTTTGTTGAGAAAATCGGGTGGTTGGATTCGTTTTATTTCTCGGTTATGTCGGTTACTACGGTTGGGTATGGTGACCGGGCTTTTAATACCTTGGCCGGTAGGCTTCTTGCTGCGATGTGGTTGCTTGTTTCTACTTTAGCGGTTGCTCGAGCGATTTTGTTTTTGGCTGAATCAAGAGTTGATAAGAGGAATAGGGAGCGGGCGAAGAAAGTTCTCGGCGAAAGCATGTCTATCTCTCAGTTCTTAGATGCGGATATCGACTGTAATGGCTGCGTGAGTAAAGCAGAGTTTGTGATATACAAACTAAAGAAGATGGACAAAATAACCGAAAAGGACATTAACCCGATCGGTTTCCAGTTTGACAAGCTTGACCGAACCAATAGTGGAAGGATTACACTTTTAGATCTTTTGGAAAGCAGCACAAAAGATTTACCCACTGCAACTTCTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0004788 GO:0005215 GO:0005216 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005887 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006996 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009108 GO:0009229 GO:0009314 GO:0009416 GO:0009507 GO:0009532 GO:0009533 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009657 GO:0009668 GO:0009705 GO:0009987 GO:0010027 GO:0010196 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0022607 GO:0022803 GO:0022838 GO:0022840 GO:0022841 GO:0022842 GO:0022857 GO:0022890 GO:0030001 GO:0030322 GO:0031090 GO:0031224 GO:0031226 GO:0031976 GO:0031984 GO:0034220 GO:0034641 GO:0042357 GO:0042391 GO:0042723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046483 GO:0046873 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051259 GO:0051260 GO:0055085 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0071704 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072527 GO:0072528 GO:0090407 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990066
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

443

Amino Acids

49.33

Weight (kDa)

8.43

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 149 - 229 8.3e-07 Ion transport protein
Ion_trans_2 PF07885 152 - 231 1.3e-15 Ion channel
Ion_trans_2 PF07885 281 - 348 1.2e-12 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 117
AccBSI CCGCTC 1 cut(s) 1078
AciI CCGC 4 cut(s) 246, 1019, 1078, 1130
AclWI GGATC 1 cut(s) 392
AcoI YGGCCR 1 cut(s) 972
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 2 cut(s) 551, 574
AfiI CCNNNNNNNGG 1 cut(s) 292
AgeI ACCGGT 2 cut(s) 307, 342
AgsI TTSAA 5 cut(s) 499, 704, 745, 751, 817
AjuI GAANNNNNNNTTGG 2 cut(s) 809, 841
AluBI AGCT 6 cut(s) 356, 446, 510, 619, 741, 1246
AluI AGCT 6 cut(s) 356, 446, 510, 619, 741, 1246
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 1 cut(s) 392
AlwNI CAGNNNCTG 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 1026
AoxI GGCC 1 cut(s) 972
ApeKI GCWGC 4 cut(s) 368, 989, 1149, 1296
AseI ATTAAT 1 cut(s) 161
AsiGI ACCGGT 2 cut(s) 307, 342
AsuC2I CCSGG 1 cut(s) 956
AsuHPI GGTGA 2 cut(s) 622, 962
AsuII TTCGAA 1 cut(s) 425
AvaI CYCGRG 1 cut(s) 1026
AxyI CCTNAGG 1 cut(s) 475
BbvI GCAGC 4 cut(s) 355, 976, 1136, 1308
BccI CCATC 4 cut(s) 215, 338, 673, 1186
BceAI ACGGC 1 cut(s) 34
BcgI CGANNNNNNTGC 2 cut(s) 982, 1016
BcnI CCSGG 1 cut(s) 956
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 1 cut(s) 186
BglII AGATCT 1 cut(s) 1282
BisI GCNGC 4 cut(s) 369, 990, 1150, 1297
BlsI GCNGC 4 cut(s) 370, 991, 1151, 1298
Bme1390I CCNGG 1 cut(s) 956
BmeT110I CYCGRG 1 cut(s) 1026
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 1 cut(s) 956
BmsI GCATC 4 cut(s) 117, 202, 532, 1117
Bpu14I TTCGAA 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 956
Bsa29I ATCGAT 2 cut(s) 210, 783
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 2 cut(s) 853, 969
BsaWI WCCGGW 3 cut(s) 100, 307, 342
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse118I RCCGGY 3 cut(s) 307, 342, 974
Bse1I ACTGG 1 cut(s) 1234
Bse21I CCTNAGG 1 cut(s) 475
BseCI ATCGAT 2 cut(s) 210, 783
BseDI CCNNGG 2 cut(s) 853, 969
BseGI GGATG 5 cut(s) 239, 388, 529, 684, 807
BseLI CCNNNNNNNGG 1 cut(s) 292
BseMII CTCAG 1 cut(s) 1130
BseNI ACTGG 1 cut(s) 1234
BseXI GCAGC 4 cut(s) 355, 976, 1136, 1308
Bsh1285I CGRYCG 1 cut(s) 1227
BshFI GGCC 1 cut(s) 974
BshTI ACCGGT 2 cut(s) 307, 342
BshVI ATCGAT 2 cut(s) 210, 783
BsiEI CGRYCG 1 cut(s) 1227
BsiHKCI CYCGRG 1 cut(s) 1026
BsiSI CCGG 6 cut(s) 101, 308, 343, 599, 955, 975
BslFI GGGAC 1 cut(s) 773
BslI CCNNNNNNNGG 1 cut(s) 292
BsmAI GTCTC 1 cut(s) 90
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 773
BsnI GGCC 1 cut(s) 974
BsoBI CYCGRG 1 cut(s) 1026
Bsp119I TTCGAA 1 cut(s) 425
Bsp143I GATC 7 cut(s) 397, 409, 697, 754, 770, 1224, 1282
BspACI CCGC 4 cut(s) 246, 1019, 1078, 1130
BspANI GGCC 1 cut(s) 974
BspCNI CTCAG 1 cut(s) 1129
BspDI ATCGAT 2 cut(s) 210, 783
BspLI GGNNCC 1 cut(s) 297
BspPI GGATC 1 cut(s) 392
BspT104I TTCGAA 1 cut(s) 425
BsrBI CCGCTC 1 cut(s) 1078
BsrFI RCCGGY 3 cut(s) 307, 342, 974
BsrI ACTGG 1 cut(s) 1234
BssAI RCCGGY 3 cut(s) 307, 342, 974
BssECI CCNNGG 2 cut(s) 853, 969
BssMI GATC 7 cut(s) 397, 409, 697, 754, 770, 1224, 1282
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 853, 969
Bst4CI ACNGT 4 cut(s) 14, 84, 940, 1142
Bst6I CTCTTC 1 cut(s) 207
BstACI GRCGYC 1 cut(s) 114
BstBI TTCGAA 1 cut(s) 425
BstC8I GCNNGC 1 cut(s) 1080
BstDEI CTNAG 4 cut(s) 475, 671, 1116, 1123
BstEII GGTNACC 2 cut(s) 610, 950
BstF5I GGATG 5 cut(s) 239, 388, 529, 684, 807
BstKTI GATC 7 cut(s) 400, 412, 700, 757, 773, 1227, 1285
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 7 cut(s) 397, 409, 697, 754, 770, 1224, 1282
BstMCI CGRYCG 1 cut(s) 1227
BstMWI GCNNNNNNNGC 1 cut(s) 368
BstNSI RCATGY 1 cut(s) 1108
BstPI GGTNACC 2 cut(s) 610, 950
BstSCI CCNGG 1 cut(s) 954
BstV1I GCAGC 4 cut(s) 355, 976, 1136, 1308
BstX2I RGATCY 1 cut(s) 1282
BstYI RGATCY 1 cut(s) 1282
Bsu15I ATCGAT 2 cut(s) 210, 783
Bsu36I CCTNAGG 1 cut(s) 475
BsuRI GGCC 1 cut(s) 974
BsuTUI ATCGAT 2 cut(s) 210, 783
BtgZI GCGATG 1 cut(s) 1007
BtsCI GGATG 5 cut(s) 239, 388, 529, 684, 807
BtsI GCAGTG 1 cut(s) 1314
BtsIMutI CAGTG 1 cut(s) 1314
Cac8I GCNNGC 1 cut(s) 1080
CaiI CAGNNNCTG 1 cut(s) 359
Cfr10I RCCGGY 3 cut(s) 307, 342, 974
ClaI ATCGAT 2 cut(s) 210, 783
Csp6I GTAC 2 cut(s) 550, 573
CspAI ACCGGT 2 cut(s) 307, 342
CviAII CATG 1 cut(s) 1105
CviQI GTAC 2 cut(s) 550, 573
DdeI CTNAG 4 cut(s) 475, 671, 1116, 1123
DpnI GATC 7 cut(s) 399, 411, 699, 756, 772, 1226, 1284
DpnII GATC 7 cut(s) 397, 409, 697, 754, 770, 1224, 1282
EaeI YGGCCR 1 cut(s) 972
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
EciI GGCGGA 1 cut(s) 235
Eco130I CCWWGG 2 cut(s) 853, 969
Eco32I GATATC 1 cut(s) 1135
Eco81I CCTNAGG 1 cut(s) 475
Eco88I CYCGRG 1 cut(s) 1026
Eco91I GGTNACC 2 cut(s) 610, 950
EcoO65I GGTNACC 2 cut(s) 610, 950
EcoRV GATATC 1 cut(s) 1135
EcoT14I CCWWGG 2 cut(s) 853, 969
EcoT22I ATGCAT 1 cut(s) 879
ErhI CCWWGG 2 cut(s) 853, 969
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 1 cut(s) 1108
FaqI GGGAC 1 cut(s) 773
FatI CATG 1 cut(s) 1104
FauI CCCGC 1 cut(s) 1071
Fnu4HI GCNGC 4 cut(s) 369, 990, 1150, 1297
FokI GGATG 5 cut(s) 226, 375, 516, 691, 814
Fsp4HI GCNGC 4 cut(s) 369, 990, 1150, 1297
FspBI CTAG 1 cut(s) 186
GluI GCNGC 4 cut(s) 369, 990, 1150, 1297
HaeIII GGCC 1 cut(s) 974
HapII CCGG 6 cut(s) 101, 308, 343, 599, 955, 975
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 1 cut(s) 1108
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HindIII AAGCTT 2 cut(s) 739, 1244
HinfI GANTC 8 cut(s) 66, 194, 266, 422, 499, 526, 905, 1049
HpaI GTTAAC 1 cut(s) 93
HpaII CCGG 6 cut(s) 101, 308, 343, 599, 955, 975
HphI GGTGA 2 cut(s) 622, 962
Hpy166II GTNNAC 3 cut(s) 93, 439, 573
Hpy188I TCNGA 4 cut(s) 126, 265, 315, 421
Hpy188III TCNNGA 7 cut(s) 186, 503, 695, 704, 758, 790, 1053
Hpy8I GTNNAC 3 cut(s) 93, 439, 573
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 3 cut(s) 240, 290, 1260
HpyCH4III ACNGT 4 cut(s) 14, 84, 940, 1142
HpyCH4IV ACGT 2 cut(s) 114, 236
HpyCH4V TGCA 3 cut(s) 371, 877, 1319
HpyF10VI GCNNNNNNNGC 1 cut(s) 368
HpyF3I CTNAG 4 cut(s) 475, 671, 1116, 1123
HpySE526I ACGT 2 cut(s) 114, 236
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 1 cut(s) 1108
KspAI GTTAAC 1 cut(s) 93
Kzo9I GATC 7 cut(s) 397, 409, 697, 754, 770, 1224, 1282
LmnI GCTCC 3 cut(s) 295, 361, 1075
LpnPI CCDG 9 cut(s) 114, 321, 356, 372, 546, 612, 968, 988, 1247
Lsp1109I GCAGC 4 cut(s) 355, 976, 1136, 1308
LweI GCATC 4 cut(s) 117, 202, 532, 1117
MaeI CTAG 1 cut(s) 186
MaeII ACGT 2 cut(s) 114, 236
MaeIII GTNAC 7 cut(s) 14, 115, 562, 610, 682, 931, 950
MalI GATC 7 cut(s) 399, 411, 699, 756, 772, 1226, 1284
MbiI CCGCTC 1 cut(s) 1078
MboI GATC 7 cut(s) 397, 409, 697, 754, 770, 1224, 1282
MboII GAAGA 5 cut(s) 157, 194, 692, 1096, 1201
MflI RGATCY 1 cut(s) 1282
MluCI AATT 2 cut(s) 120, 334
MlyI GAGTC 1 cut(s) 520
MmeI TCCRAC 2 cut(s) 699, 882
MnlI CCTC 7 cut(s) 253, 265, 287, 723, 792, 798, 1059
Mph1103I ATGCAT 1 cut(s) 879
MseI TTAA 5 cut(s) 92, 150, 161, 963, 1218
MslI CAYNNNNRTG 1 cut(s) 393
MspI CCGG 6 cut(s) 101, 308, 343, 599, 955, 975
MspR9I CCNGG 1 cut(s) 956
MwoI GCNNNNNNNGC 1 cut(s) 368
NciI CCSGG 1 cut(s) 956
NdeII GATC 7 cut(s) 397, 409, 697, 754, 770, 1224, 1282
NlaIII CATG 1 cut(s) 1108
NlaIV GGNNCC 1 cut(s) 297
NmeAIII GCCGAG 1 cut(s) 1074
NmuCI GTSAC 3 cut(s) 115, 610, 950
NsiI ATGCAT 1 cut(s) 879
NspI RCATGY 1 cut(s) 1108
NspV TTCGAA 1 cut(s) 425
PaeR7I CTCGAG 1 cut(s) 1026
PcsI WCGNNNNNNNCGW 3 cut(s) 18, 174, 572
PfeI GAWTC 7 cut(s) 66, 194, 266, 422, 499, 905, 1049
PinAI ACCGGT 2 cut(s) 307, 342
PkrI GCNGC 4 cut(s) 370, 991, 1151, 1298
Ple19I CGATCG 1 cut(s) 1227
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
PshBI ATTAAT 1 cut(s) 161
PspEI GGTNACC 2 cut(s) 610, 950
PspN4I GGNNCC 1 cut(s) 297
PspXI VCTCGAGB 1 cut(s) 1026
PstNI CAGNNNCTG 1 cut(s) 359
PsuI RGATCY 1 cut(s) 1282
PvuI CGATCG 1 cut(s) 1227
RsaI GTAC 2 cut(s) 551, 574
RsaNI GTAC 2 cut(s) 550, 573
RseI CAYNNNNRTG 1 cut(s) 393
SaqAI TTAA 5 cut(s) 92, 150, 161, 963, 1218
SatI GCNGC 4 cut(s) 369, 990, 1150, 1297
Sau3AI GATC 7 cut(s) 397, 409, 697, 754, 770, 1224, 1282
SchI GAGTC 1 cut(s) 520
ScrFI CCNGG 1 cut(s) 956
SfaNI GCATC 4 cut(s) 117, 202, 532, 1117
Sfr274I CTCGAG 1 cut(s) 1026
SfuI TTCGAA 1 cut(s) 425
SlaI CTCGAG 1 cut(s) 1026
SmiMI CAYNNNNRTG 1 cut(s) 393
SmlI CTYRAG 1 cut(s) 1026
SmoI CTYRAG 1 cut(s) 1026
Sse9I AATT 2 cut(s) 120, 334
SsiI CCGC 4 cut(s) 246, 1019, 1078, 1130
SspMI CTAG 1 cut(s) 186
StyD4I CCNGG 1 cut(s) 954
StyI CCWWGG 2 cut(s) 853, 969
TaaI ACNGT 4 cut(s) 14, 84, 940, 1142
TaiI ACGT 2 cut(s) 117, 239
TaqI TCGA 7 cut(s) 210, 425, 502, 626, 783, 1027, 1137
TaqII GACCGA 1 cut(s) 1266
TasI AATT 2 cut(s) 120, 334
TatI WGTACW 1 cut(s) 549
TfiI GAWTC 7 cut(s) 66, 194, 266, 422, 499, 905, 1049
Tru1I TTAA 5 cut(s) 92, 150, 161, 963, 1218
Tru9I TTAA 5 cut(s) 92, 150, 161, 963, 1218
TscAI CASTG 1 cut(s) 1321
TseFI GTSAC 3 cut(s) 115, 610, 950
TseI GCWGC 4 cut(s) 368, 989, 1149, 1296
Tsp45I GTSAC 3 cut(s) 115, 610, 950
TspDTI ATGAA 3 cut(s) 71, 162, 750
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 1 cut(s) 1321
VspI ATTAAT 1 cut(s) 161
XbaI TCTAGA 1 cut(s) 185
XceI RCATGY 1 cut(s) 1108
XhoI CTCGAG 1 cut(s) 1026
XspI CTAG 1 cut(s) 186
ZraI GACGTC 1 cut(s) 115
Zsp2I ATGCAT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.