RchiOBHm_Chr5g0013121

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009345
Physical Location & Seq
Forward (+)
8888953 .. 8893904
4952 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29366

Sequence Viewer

Length: 915 bp
ATGGCGGTTTTGAACGAGGTGAACCGCCGGTCAGGGACTAAGCCACAAGTGAGTTCACAGTCGATTGTTAGGCAAGCGGTGATTCTTCTTGTTGCGTATTTGACTCTGGGGGTGATTATATATTGGTTTAACCGTGATCATTTTGCTGCGAGTGAGACTCACCCTGTTGTGGATGCTTTGTATTTTTGTATTGTGACAATGTGCACTATTGGGTATGGGGATATCACGCCTAAGACCACGGCTACGAAGCTGTTTTCGATAATGTTTGTGTTGGTGGGGTTCGGGTTTATTGATATTTTGCTTAGTGGGATGGTGGCTTATGTGCTTGATTTGCAAGAGAGTTATTTGCTGAGGAGTGTGAAGGGGGTGGGGGAGAAGAAGGAGTCGTATATAATTGATGTGAAGAAGGGGAGGATGAGGATTAGGATGAAGGTGGGATTGGCATTGGGGGTTGTGGTGCTTTGTATTGGTGTTGGTGCTATGGTTATGCATTATGTGGAGAAGCTCGGATGGTTGGATTCGTTTTATCTGTCGGTGATGTCTGTTACGACTGTGGGGTATGGTGACCGGGCGTTCCAGTCTATGGAGGGTCGAATCTTTGCATCAATCTGGTTGCTTGTGTCCACACTCGCGGTTGCTAGAGCCTTTCTGTATTTGGCTGAGGCAAGGGTGGATAAACGGCATAGGAAGATGGCAAAGTGGGTTCTTGGCCAGGATATGACTGTTTCTGAGTTCCTTGCTGCTGACATTGACAATAATGGCTTTGTGAGCAAGTCAGAGTATGTTGTATACAAACTCAAGGAGATGGGAAAGGTATCAGAGAAAGATATTATGCAGATCTCCAACAAATTTGATAGGCTAGACGCTGGGAACTGCGGAAAGATAACCCTTGCTGATCTTATGAGTCGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0004788 GO:0005215 GO:0005216 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005887 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006996 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009108 GO:0009229 GO:0009314 GO:0009416 GO:0009507 GO:0009532 GO:0009533 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009657 GO:0009668 GO:0009705 GO:0009987 GO:0010027 GO:0010196 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0022607 GO:0022803 GO:0022838 GO:0022840 GO:0022841 GO:0022842 GO:0022857 GO:0022890 GO:0030001 GO:0030322 GO:0031090 GO:0031224 GO:0031226 GO:0031976 GO:0031984 GO:0034220 GO:0034641 GO:0042357 GO:0042391 GO:0042723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046483 GO:0046873 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051259 GO:0051260 GO:0055085 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0071704 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072527 GO:0072528 GO:0090407 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990066
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

304

Amino Acids

34.0

Weight (kDa)

9.2

Isoelectric Point (pI)

31.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 27 - 105 1e-06 Ion transport protein
Ion_trans_2 PF07885 28 - 107 7.9e-17 Ion channel
Ion_trans_2 PF07885 152 - 221 6.6e-12 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 583
AccI GTMKAC 1 cut(s) 789
AccII CGCG 1 cut(s) 632
AciI CCGC 5 cut(s) 5, 25, 77, 632, 876
AcoI YGGCCR 1 cut(s) 709
AcsI RAATTY 1 cut(s) 848
AfiI CCNNNNNNNGG 2 cut(s) 169, 583
AgsI TTSAA 1 cut(s) 13
AjnI CCWGG 1 cut(s) 711
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
AluBI AGCT 2 cut(s) 250, 505
AluI AGCT 2 cut(s) 250, 505
Alw21I GWGCWC 1 cut(s) 206
Alw26I GTCTC 1 cut(s) 149
Alw44I GTGCAC 1 cut(s) 202
AoxI GGCC 1 cut(s) 709
ApaLI GTGCAC 1 cut(s) 202
ApeKI GCWGC 2 cut(s) 146, 740
ApoI RAATTY 1 cut(s) 848
AsuC2I CCSGG 1 cut(s) 569
AsuHPI GGTGA 6 cut(s) 31, 91, 124, 152, 547, 575
BaeGI GKGCMC 1 cut(s) 206
BalI TGGCCA 1 cut(s) 711
Bbv12I GWGCWC 1 cut(s) 206
BbvCI CCTCAGC 2 cut(s) 350, 660
BbvI GCAGC 2 cut(s) 133, 727
BccI CCATC 4 cut(s) 304, 504, 685, 799
BceAI ACGGC 2 cut(s) 255, 695
BciT130I CCWGG 1 cut(s) 713
BclI TGATCA 1 cut(s) 136
BcnI CCSGG 1 cut(s) 569
BcoDI GTCTC 1 cut(s) 149
BfaI CTAG 2 cut(s) 639, 860
BglII AGATCT 1 cut(s) 837
BisI GCNGC 2 cut(s) 147, 741
BlsI GCNGC 2 cut(s) 148, 742
Bme1390I CCNGG 2 cut(s) 569, 713
BmrFI CCNGG 2 cut(s) 569, 713
BmsI GCATC 2 cut(s) 163, 611
BplI GAGNNNNNCTC 2 cut(s) 142, 174
Bpu10I CCTNAGC 2 cut(s) 350, 660
BpuEI CTTGAG 1 cut(s) 782
BpuMI CCSGG 1 cut(s) 569
BsaJI CCNNGG 1 cut(s) 237
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 583
Bse118I RCCGGY 1 cut(s) 27
Bse1I ACTGG 1 cut(s) 577
BseBI CCWGG 1 cut(s) 713
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 5 cut(s) 178, 315, 420, 432, 515
BseLI CCNNNNNNNGG 2 cut(s) 169, 583
BseMII CTCAG 3 cut(s) 341, 651, 720
BseNI ACTGG 1 cut(s) 577
BseRI GAGGAG 1 cut(s) 367
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 2 cut(s) 133, 727
BseYI CCCAGC 1 cut(s) 866
Bsh1236I CGCG 1 cut(s) 632
BshFI GGCC 1 cut(s) 711
BsiHKAI GWGCWC 1 cut(s) 206
BsiSI CCGG 2 cut(s) 28, 568
BslFI GGGAC 1 cut(s) 49
BslI CCNNNNNNNGG 2 cut(s) 169, 583
BsmAI GTCTC 1 cut(s) 149
BsmFI GGGAC 1 cut(s) 49
BsnI GGCC 1 cut(s) 711
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 3 cut(s) 136, 837, 895
BspACI CCGC 5 cut(s) 5, 25, 77, 632, 876
BspANI GGCC 1 cut(s) 711
BspCNI CTCAG 3 cut(s) 342, 652, 721
BspFNI CGCG 1 cut(s) 632
BsrFI RCCGGY 1 cut(s) 27
BsrI ACTGG 1 cut(s) 577
BssAI RCCGGY 1 cut(s) 27
BssECI CCNNGG 1 cut(s) 237
BssMI GATC 3 cut(s) 136, 837, 895
BssNAI GTATAC 1 cut(s) 790
Bst1107I GTATAC 1 cut(s) 790
Bst2UI CCWGG 1 cut(s) 713
Bst4CI ACNGT 4 cut(s) 60, 134, 553, 724
BstC8I GCNNGC 1 cut(s) 75
BstDEI CTNAG 6 cut(s) 39, 231, 302, 350, 660, 729
BstDSI CCRYGG 1 cut(s) 237
BstEII GGTNACC 1 cut(s) 563
BstF5I GGATG 5 cut(s) 178, 315, 420, 432, 515
BstFNI CGCG 1 cut(s) 632
BstKTI GATC 3 cut(s) 139, 840, 898
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 3 cut(s) 136, 837, 895
BstMWI GCNNNNNNNGC 2 cut(s) 331, 768
BstNI CCWGG 1 cut(s) 713
BstPI GGTNACC 1 cut(s) 563
BstSCI CCNGG 2 cut(s) 567, 711
BstSLI GKGCMC 1 cut(s) 206
BstUI CGCG 1 cut(s) 632
BstV1I GCAGC 2 cut(s) 133, 727
BstX2I RGATCY 1 cut(s) 837
BstYI RGATCY 1 cut(s) 837
BstZ17I GTATAC 1 cut(s) 790
BsuRI GGCC 1 cut(s) 711
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 5 cut(s) 178, 315, 420, 432, 515
Cac8I GCNNGC 1 cut(s) 75
Cfr10I RCCGGY 1 cut(s) 27
CseI GACGC 1 cut(s) 872
DdeI CTNAG 6 cut(s) 39, 231, 302, 350, 660, 729
DpnI GATC 3 cut(s) 138, 839, 897
DpnII GATC 3 cut(s) 136, 837, 895
EaeI YGGCCR 1 cut(s) 709
Eco32I GATATC 1 cut(s) 223
Eco91I GGTNACC 1 cut(s) 563
EcoO65I GGTNACC 1 cut(s) 563
EcoRII CCWGG 1 cut(s) 711
EcoRV GATATC 1 cut(s) 223
EcoT22I ATGCAT 1 cut(s) 492
FaqI GGGAC 1 cut(s) 49
FbaI TGATCA 1 cut(s) 136
FblI GTMKAC 1 cut(s) 789
Fnu4HI GCNGC 2 cut(s) 147, 741
FokI GGATG 5 cut(s) 185, 322, 427, 439, 522
Fsp4HI GCNGC 2 cut(s) 147, 741
FspBI CTAG 2 cut(s) 639, 860
GluI GCNGC 2 cut(s) 147, 741
GsaI CCCAGC 1 cut(s) 870
HaeIII GGCC 1 cut(s) 711
HapII CCGG 2 cut(s) 28, 568
HgaI GACGC 1 cut(s) 872
HinfI GANTC 7 cut(s) 82, 103, 157, 383, 518, 594, 904
HpaII CCGG 2 cut(s) 28, 568
HphI GGTGA 6 cut(s) 31, 91, 124, 152, 547, 575
Hpy166II GTNNAC 5 cut(s) 22, 56, 204, 624, 790
Hpy188I TCNGA 4 cut(s) 509, 730, 778, 820
Hpy8I GTNNAC 5 cut(s) 22, 56, 204, 624, 790
HpyAV CCTTC 4 cut(s) 355, 373, 400, 424
HpyCH4III ACNGT 4 cut(s) 60, 134, 553, 724
HpyCH4V TGCA 5 cut(s) 204, 334, 490, 602, 835
HpyF10VI GCNNNNNNNGC 2 cut(s) 331, 768
HpyF3I CTNAG 6 cut(s) 39, 231, 302, 350, 660, 729
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 3 cut(s) 136, 837, 895
Lsp1109I GCAGC 2 cut(s) 133, 727
LweI GCATC 2 cut(s) 163, 611
MaeI CTAG 2 cut(s) 639, 860
MaeIII GTNAC 3 cut(s) 193, 544, 563
MalI GATC 3 cut(s) 138, 839, 897
MboI GATC 3 cut(s) 136, 837, 895
MboII GAAGA 4 cut(s) 77, 388, 415, 700
MflI RGATCY 1 cut(s) 837
MhlI GDGCHC 1 cut(s) 206
MlsI TGGCCA 1 cut(s) 711
MluCI AATT 2 cut(s) 393, 848
MluNI TGGCCA 1 cut(s) 711
MlyI GAGTC 4 cut(s) 97, 151, 392, 913
MmeI TCCRAC 2 cut(s) 495, 867
MnlI CCTC 6 cut(s) 10, 345, 405, 411, 580, 655
Mox20I TGGCCA 1 cut(s) 711
Mph1103I ATGCAT 1 cut(s) 492
MscI TGGCCA 1 cut(s) 711
MseI TTAA 1 cut(s) 129
Msp20I TGGCCA 1 cut(s) 711
MspI CCGG 2 cut(s) 28, 568
MspR9I CCNGG 2 cut(s) 569, 713
MvaI CCWGG 1 cut(s) 713
MvnI CGCG 1 cut(s) 632
MwoI GCNNNNNNNGC 2 cut(s) 331, 768
NciI CCSGG 1 cut(s) 569
NdeII GATC 3 cut(s) 136, 837, 895
NmuCI GTSAC 2 cut(s) 193, 563
NsiI ATGCAT 1 cut(s) 492
PfeI GAWTC 3 cut(s) 82, 518, 594
PflMI CCANNNNNTGG 1 cut(s) 583
PkrI GCNGC 2 cut(s) 148, 742
PleI GAGTC 4 cut(s) 97, 151, 391, 912
PpsI GAGTC 4 cut(s) 97, 151, 391, 912
Psp6I CCWGG 1 cut(s) 711
PspEI GGTNACC 1 cut(s) 563
PspFI CCCAGC 1 cut(s) 866
PspGI CCWGG 1 cut(s) 711
PsuI RGATCY 1 cut(s) 837
SaqAI TTAA 1 cut(s) 129
SatI GCNGC 2 cut(s) 147, 741
Sau3AI GATC 3 cut(s) 136, 837, 895
SchI GAGTC 4 cut(s) 97, 151, 392, 913
ScrFI CCNGG 2 cut(s) 569, 713
SduI GDGCHC 1 cut(s) 206
SetI ASST 5 cut(s) 21, 252, 435, 507, 816
SfaNI GCATC 2 cut(s) 163, 611
SmlI CTYRAG 1 cut(s) 797
SmoI CTYRAG 1 cut(s) 797
Sse9I AATT 2 cut(s) 393, 848
SsiI CCGC 5 cut(s) 5, 25, 77, 632, 876
SspMI CTAG 2 cut(s) 639, 860
StyD4I CCNGG 2 cut(s) 567, 711
TaaI ACNGT 4 cut(s) 60, 134, 553, 724
TaqI TCGA 3 cut(s) 62, 257, 592
TasI AATT 2 cut(s) 393, 848
TfiI GAWTC 3 cut(s) 82, 518, 594
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TseFI GTSAC 2 cut(s) 193, 563
TseI GCWGC 2 cut(s) 146, 740
Tsp45I GTSAC 2 cut(s) 193, 563
TspDTI ATGAA 1 cut(s) 443
Van91I CCANNNNNTGG 1 cut(s) 583
VneI GTGCAC 1 cut(s) 202
XapI RAATTY 1 cut(s) 848
XmiI GTMKAC 1 cut(s) 789
XspI CTAG 2 cut(s) 639, 860
Zsp2I ATGCAT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.