MD05G1290400.v1.1

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009345
Physical Location & Seq
Reverse (-)
42225001 .. 42228071
3071 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1290400.v1.1.491

Sequence Viewer

Length: 1290 bp
ATGGAGAAAGAGCCATTACTCCCATACGTAGGCAGCCCTAGAAGAAAGCTGTCACCTTTACCGATTCTCTGCCCACTTCCAGAAGAGGATGAATTCACTATCCCTTTAACCCCTTCGGAATTCAAAGACCGTATAATCTTTGGCCCTGCAACTGCTTCGTCGCAGGATTCTTGTCCTCTCGTCGATGCATTGACTCTGCCTATCAATTCTCCAAGAACTTCAAGACCCCCTTCGTCGTCTTCTCTGTTGGACTCCGTAGCCACAATCTCGCAGGACCCACAAACCCAACAGCCACTCCTCCAATCATGGCTCATCGACCCCAATTACTCATGGACCAAAACCAACCTCCACCGCTCCAAAACAGCTCCTGCCATGGCCGTGATCAACGAGGTGGCGAACCGGCGCTCTGTTCCCAGACCCCAATTCGGTTCACAGTGGATTGTAAGACAAGCTGTTGTACTTCTTGTTATATATCTTGCATTGGGTGTGACTATATATTGGTTTAACCGTGATAATTTCGCTGCGTCGGAGACTCACCCTGTGGTTGATGCGTTATACTTTTGTATTGTGACAATGTGCACAATTGGGTATGGAGATATTACGCCTACTACTACAGCAACCAAGTTGTTTTCGATAATGTTTGTGTTGGTGGGGTTTGGATTCATTGATATTTTGCTTAGTGGGATGGTTAGTTATGTGCTTGATTTGCAAGAGACTTATTTGTTGAGGAGCCTTAAGGATGTGGGTGAGAAAAAGGAGTCCTACATATTTGATGTTAAGAAGGGGAGGATGAGGATTAGGATGAAGGTGGGATTGGCATTGGGAGTTGTGGTTCTTTGTATTGGGATCGGTGTGGGTGTGATGCATTTTGTGGAAAAGCTTGGATGGTTGGATTCGTTTTATCTTTCGGTTATGTCAGTTACCACTGTAGGGTATGGTGACCGGGCTTTCCAGTCTTTGCCAGGACGTATCTTTGCTTCTATTTGGTTGCTTGTGTCGACACTTGCAGTTGCTCGAGCATTTCTGTTTTTGGCTGAGGCTAGGGTGGATAAGCGGCATAGGAGGATGGCGAAGTGGGTTCTTGGTCAGGATATGACTGTTTCTGAGTTTCTTGCTGCTGACATTGACAATAATGGTTTTGTGAGTAAGTCGGAATATGTCGTATACAAACTCAAGGAGATGGGAAAGGTATCAGAGAAAGATATTATGCAGATCTGCACCCAATTCGATAGGCTAGACGCTGGGAACTGCGGAAAGATAAGCCTTGCTGATCTTTTGAGTCGACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0004788 GO:0005215 GO:0005216 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005887 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006996 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009108 GO:0009229 GO:0009314 GO:0009416 GO:0009507 GO:0009532 GO:0009533 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009657 GO:0009668 GO:0009705 GO:0009987 GO:0010027 GO:0010196 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0022607 GO:0022803 GO:0022838 GO:0022840 GO:0022841 GO:0022842 GO:0022857 GO:0022890 GO:0030001 GO:0030322 GO:0031090 GO:0031224 GO:0031226 GO:0031976 GO:0031984 GO:0034220 GO:0034641 GO:0042357 GO:0042391 GO:0042723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046483 GO:0046873 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051259 GO:0051260 GO:0055085 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0071704 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072527 GO:0072528 GO:0090407 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990066
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

430

Amino Acids

47.83

Weight (kDa)

8.56

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 152 - 233 5.2e-07 Ion transport protein
Ion_trans_2 PF07885 154 - 233 6.8e-16 Ion channel
Ion_trans_2 PF07885 277 - 345 3.4e-11 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 354
AccI GTMKAC 3 cut(s) 998, 1164, 1282
AciI CCGC 3 cut(s) 352, 1054, 1251
AclWI GGATC 1 cut(s) 854
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 2 cut(s) 92, 119
AdeI CACNNNGTG 1 cut(s) 541
AfaI GTAC 1 cut(s) 459
AfiI CCNNNNNNNGG 3 cut(s) 29, 425, 930
AflII CTTAAG 1 cut(s) 734
AgsI TTSAA 2 cut(s) 124, 222
AjnI CCWGG 1 cut(s) 961
AjuI GAANNNNNNNTTGG 2 cut(s) 797, 829
AloI GAACNNNNNNTCC 2 cut(s) 816, 848
AluBI AGCT 4 cut(s) 49, 365, 452, 880
AluI AGCT 4 cut(s) 49, 365, 452, 880
Alw21I GWGCWC 1 cut(s) 581
Alw26I GTCTC 2 cut(s) 524, 707
Alw44I GTGCAC 1 cut(s) 577
AlwI GGATC 1 cut(s) 854
AlwNI CAGNNNCTG 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 1014
AoxI GGCC 2 cut(s) 142, 375
ApaLI GTGCAC 1 cut(s) 577
ApeKI GCWGC 3 cut(s) 33, 521, 1115
ApoI RAATTY 2 cut(s) 92, 119
AspLEI GCGC 1 cut(s) 405
AspS9I GGNCC 3 cut(s) 143, 274, 333
AsuC2I CCSGG 1 cut(s) 944
AsuHPI GGTGA 4 cut(s) 45, 527, 758, 950
AvaI CYCGRG 1 cut(s) 1014
AvaII GGWCC 2 cut(s) 274, 333
BaeGI GKGCMC 1 cut(s) 581
BarI GAAGNNNNNNTAC 2 cut(s) 961, 993
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 581
BbvCI CCTCAGC 1 cut(s) 1035
BbvI GCAGC 3 cut(s) 45, 508, 1102
BccI CCATC 4 cut(s) 679, 879, 1060, 1174
BceAI ACGGC 1 cut(s) 362
BcgI CGANNNNNNTGC 4 cut(s) 138, 172, 1207, 1241
BciT130I CCWGG 1 cut(s) 963
BclI TGATCA 1 cut(s) 381
BcnI CCSGG 1 cut(s) 944
BcoDI GTCTC 2 cut(s) 524, 707
BfaI CTAG 3 cut(s) 39, 1041, 1235
BfmI CTRYAG 2 cut(s) 612, 927
BfoI RGCGCY 1 cut(s) 406
BfrI CTTAAG 1 cut(s) 734
BglII AGATCT 1 cut(s) 1212
BisI GCNGC 4 cut(s) 34, 522, 1055, 1116
BlsI GCNGC 4 cut(s) 35, 523, 1056, 1117
Bme1390I CCNGG 2 cut(s) 944, 963
Bme18I GGWCC 2 cut(s) 274, 333
BmeT110I CYCGRG 1 cut(s) 1014
BmgT120I GGNCC 3 cut(s) 143, 274, 333
BmiI GGNNCC 2 cut(s) 276, 731
BmrFI CCNGG 2 cut(s) 944, 963
BmsI GCATC 3 cut(s) 175, 538, 852
BpiI GAAGAC 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 1 cut(s) 1157
BpuMI CCSGG 1 cut(s) 944
BsaAI YACGTR 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 372
BsaXI ACNNNNNCTCC 4 cut(s) 279, 309, 816, 846
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 425, 930
Bse118I RCCGGY 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 952
BseBI CCWGG 1 cut(s) 963
BseDI CCNNGG 1 cut(s) 372
BseGI GGATG 7 cut(s) 94, 690, 745, 795, 807, 890, 1071
BseLI CCNNNNNNNGG 3 cut(s) 29, 425, 930
BseMII CTCAG 2 cut(s) 1026, 1095
BseNI ACTGG 1 cut(s) 952
BseRI GAGGAG 2 cut(s) 287, 742
BseSI GKGCMC 1 cut(s) 581
BseXI GCAGC 3 cut(s) 45, 508, 1102
BseYI CCCAGC 1 cut(s) 1241
BsgI GTGCAG 1 cut(s) 1201
BshFI GGCC 2 cut(s) 144, 377
BsiHKAI GWGCWC 1 cut(s) 581
BsiHKCI CYCGRG 1 cut(s) 1014
BsiSI CCGG 2 cut(s) 400, 943
BslI CCNNNNNNNGG 3 cut(s) 29, 425, 930
BsmAI GTCTC 2 cut(s) 524, 707
BsnI GGCC 2 cut(s) 144, 377
BsoBI CYCGRG 1 cut(s) 1014
Bsp1286I GDGCHC 1 cut(s) 581
Bsp143I GATC 4 cut(s) 381, 846, 1212, 1270
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 3 cut(s) 352, 1054, 1251
BspANI GGCC 2 cut(s) 144, 377
BspCNI CTCAG 2 cut(s) 1027, 1096
BspLI GGNNCC 2 cut(s) 276, 731
BspPI GGATC 1 cut(s) 854
BspTI CTTAAG 1 cut(s) 734
BsrBI CCGCTC 1 cut(s) 354
BsrFI RCCGGY 1 cut(s) 399
BsrI ACTGG 1 cut(s) 952
BssAI RCCGGY 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 372
BssMI GATC 4 cut(s) 381, 846, 1212, 1270
BssNAI GTATAC 1 cut(s) 1165
BssT1I CCWWGG 1 cut(s) 372
Bst1107I GTATAC 1 cut(s) 1165
Bst2UI CCWGG 1 cut(s) 963
Bst4CI ACNGT 5 cut(s) 131, 435, 509, 928, 1099
Bst6I CTCTTC 1 cut(s) 78
BstAFI CTTAAG 1 cut(s) 734
BstBAI YACGTR 1 cut(s) 28
BstDEI CTNAG 3 cut(s) 677, 1035, 1104
BstDSI CCRYGG 1 cut(s) 372
BstEII GGTNACC 1 cut(s) 938
BstF5I GGATG 7 cut(s) 94, 690, 745, 795, 807, 890, 1071
BstH2I RGCGCY 1 cut(s) 406
BstHHI GCGC 1 cut(s) 405
BstKTI GATC 4 cut(s) 384, 849, 1215, 1273
BstMAI GTCTC 2 cut(s) 524, 707
BstMBI GATC 4 cut(s) 381, 846, 1212, 1270
BstMWI GCNNNNNNNGC 1 cut(s) 706
BstNI CCWGG 1 cut(s) 963
BstPI GGTNACC 1 cut(s) 938
BstSCI CCNGG 2 cut(s) 942, 961
BstSFI CTRYAG 2 cut(s) 612, 927
BstSLI GKGCMC 1 cut(s) 581
BstSNI TACGTA 1 cut(s) 28
BstV1I GCAGC 3 cut(s) 45, 508, 1102
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 1212
BstYI RGATCY 1 cut(s) 1212
BstZ17I GTATAC 1 cut(s) 1165
BsuRI GGCC 2 cut(s) 144, 377
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 7 cut(s) 94, 690, 745, 795, 807, 890, 1071
BtsIMutI CAGTG 2 cut(s) 440, 924
CaiI CAGNNNCTG 1 cut(s) 368
CfoI GCGC 1 cut(s) 405
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 3 cut(s) 143, 274, 333
CseI GACGC 2 cut(s) 513, 1247
Csp6I GTAC 1 cut(s) 458
CviAII CATG 3 cut(s) 306, 330, 373
CviQI GTAC 1 cut(s) 458
DdeI CTNAG 3 cut(s) 677, 1035, 1104
DpnI GATC 4 cut(s) 383, 848, 1214, 1272
DpnII GATC 4 cut(s) 381, 846, 1212, 1270
DraIII CACNNNGTG 1 cut(s) 541
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 1 cut(s) 78
EarI CTCTTC 1 cut(s) 78
Eco105I TACGTA 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 372
Eco47I GGWCC 2 cut(s) 274, 333
Eco88I CYCGRG 1 cut(s) 1014
Eco91I GGTNACC 1 cut(s) 938
EcoO109I RGGNCCY 1 cut(s) 274
EcoO65I GGTNACC 1 cut(s) 938
EcoRI GAATTC 2 cut(s) 92, 119
EcoRII CCWGG 1 cut(s) 961
EcoT14I CCWWGG 1 cut(s) 372
EcoT22I ATGCAT 2 cut(s) 190, 867
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 3 cut(s) 309, 333, 376
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FatI CATG 3 cut(s) 305, 329, 372
FbaI TGATCA 1 cut(s) 381
FblI GTMKAC 3 cut(s) 998, 1164, 1282
Fnu4HI GCNGC 4 cut(s) 34, 522, 1055, 1116
FokI GGATG 7 cut(s) 101, 697, 752, 802, 814, 897, 1078
Fsp4HI GCNGC 4 cut(s) 34, 522, 1055, 1116
FspBI CTAG 3 cut(s) 39, 1041, 1235
GlaI GCGC 1 cut(s) 404
GluI GCNGC 4 cut(s) 34, 522, 1055, 1116
GsaI CCCAGC 1 cut(s) 1245
HaeII RGCGCY 1 cut(s) 406
HaeIII GGCC 2 cut(s) 144, 377
HapII CCGG 2 cut(s) 400, 943
HgaI GACGC 2 cut(s) 513, 1247
HhaI GCGC 1 cut(s) 405
Hin1II CATG 3 cut(s) 309, 333, 376
Hin6I GCGC 1 cut(s) 403
HinP1I GCGC 1 cut(s) 403
HincII GTYRAC 2 cut(s) 999, 1283
HindII GTYRAC 2 cut(s) 999, 1283
HindIII AAGCTT 1 cut(s) 878
HinfI GANTC 9 cut(s) 64, 167, 193, 251, 532, 660, 758, 893, 1279
HpaII CCGG 2 cut(s) 400, 943
HphI GGTGA 4 cut(s) 45, 527, 758, 950
Hpy166II GTNNAC 5 cut(s) 431, 579, 999, 1165, 1283
Hpy188I TCNGA 5 cut(s) 118, 529, 1105, 1153, 1195
Hpy188III TCNNGA 3 cut(s) 80, 222, 1088
Hpy8I GTNNAC 5 cut(s) 431, 579, 999, 1165, 1283
Hpy99I CGWCG 4 cut(s) 163, 185, 238, 529
HpyAV CCTTC 4 cut(s) 123, 240, 775, 799
HpyCH4III ACNGT 5 cut(s) 131, 435, 509, 928, 1099
HpyCH4IV ACGT 2 cut(s) 27, 967
HpyCH4V TGCA 9 cut(s) 149, 188, 479, 579, 709, 865, 1007, 1210, 1218
HpyF10VI GCNNNNNNNGC 1 cut(s) 706
HpyF3I CTNAG 3 cut(s) 677, 1035, 1104
HpySE526I ACGT 2 cut(s) 27, 967
Hsp92II CATG 3 cut(s) 309, 333, 376
HspAI GCGC 1 cut(s) 403
Ksp22I TGATCA 1 cut(s) 381
Kzo9I GATC 4 cut(s) 381, 846, 1212, 1270
LmnI GCTCC 3 cut(s) 359, 370, 729
Lsp1109I GCAGC 3 cut(s) 45, 508, 1102
LweI GCATC 3 cut(s) 175, 538, 852
MaeI CTAG 3 cut(s) 39, 1041, 1235
MaeII ACGT 2 cut(s) 27, 967
MaeIII GTNAC 5 cut(s) 51, 487, 568, 919, 938
MalI GATC 4 cut(s) 383, 848, 1214, 1272
MbiI CCGCTC 1 cut(s) 354
MboI GATC 4 cut(s) 381, 846, 1212, 1270
MboII GAAGA 3 cut(s) 54, 95, 231
MfeI CAATTG 1 cut(s) 582
MflI RGATCY 1 cut(s) 1212
MhlI GDGCHC 1 cut(s) 581
MluCI AATT 8 cut(s) 92, 119, 205, 322, 422, 514, 582, 1223
MlyI GAGTC 5 cut(s) 187, 245, 526, 767, 1288
MmeI TCCRAC 4 cut(s) 228, 507, 870, 1131
Mph1103I ATGCAT 2 cut(s) 190, 867
MseI TTAA 4 cut(s) 107, 504, 735, 777
MslI CAYNNNNRTG 1 cut(s) 377
MspCI CTTAAG 1 cut(s) 734
MspI CCGG 2 cut(s) 400, 943
MspR9I CCNGG 2 cut(s) 944, 963
MunI CAATTG 1 cut(s) 582
MvaI CCWGG 1 cut(s) 963
MwoI GCNNNNNNNGC 1 cut(s) 706
NciI CCSGG 1 cut(s) 944
NcoI CCATGG 1 cut(s) 372
NdeII GATC 4 cut(s) 381, 846, 1212, 1270
NlaIII CATG 3 cut(s) 309, 333, 376
NlaIV GGNNCC 2 cut(s) 276, 731
NmuCI GTSAC 4 cut(s) 51, 487, 568, 938
NsiI ATGCAT 2 cut(s) 190, 867
PaeR7I CTCGAG 1 cut(s) 1014
PfeI GAWTC 4 cut(s) 64, 167, 660, 893
PkrI GCNGC 4 cut(s) 35, 523, 1056, 1117
PleI GAGTC 5 cut(s) 187, 245, 526, 766, 1287
PpsI GAGTC 5 cut(s) 187, 245, 526, 766, 1287
Ppu21I YACGTR 1 cut(s) 28
PpuMI RGGWCCY 1 cut(s) 274
Psp5II RGGWCCY 1 cut(s) 274
Psp6I CCWGG 1 cut(s) 961
PspEI GGTNACC 1 cut(s) 938
PspFI CCCAGC 1 cut(s) 1241
PspGI CCWGG 1 cut(s) 961
PspN4I GGNNCC 2 cut(s) 276, 731
PspPI GGNCC 3 cut(s) 143, 274, 333
PspPPI RGGWCCY 1 cut(s) 274
PspXI VCTCGAGB 1 cut(s) 1014
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 1 cut(s) 1212
RsaI GTAC 1 cut(s) 459
RsaNI GTAC 1 cut(s) 458
RseI CAYNNNNRTG 1 cut(s) 377
SalI GTCGAC 2 cut(s) 997, 1281
SaqAI TTAA 4 cut(s) 107, 504, 735, 777
SatI GCNGC 4 cut(s) 34, 522, 1055, 1116
Sau3AI GATC 4 cut(s) 381, 846, 1212, 1270
Sau96I GGNCC 3 cut(s) 143, 274, 333
SchI GAGTC 5 cut(s) 187, 245, 526, 767, 1288
ScrFI CCNGG 2 cut(s) 944, 963
SduI GDGCHC 1 cut(s) 581
SfaNI GCATC 3 cut(s) 175, 538, 852
SfcI CTRYAG 2 cut(s) 612, 927
Sfr274I CTCGAG 1 cut(s) 1014
SinI GGWCC 2 cut(s) 274, 333
SlaI CTCGAG 1 cut(s) 1014
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 3 cut(s) 734, 1014, 1172
SmoI CTYRAG 3 cut(s) 734, 1014, 1172
SnaBI TACGTA 1 cut(s) 28
Sse9I AATT 8 cut(s) 92, 119, 205, 322, 422, 514, 582, 1223
SsiI CCGC 3 cut(s) 352, 1054, 1251
SspMI CTAG 3 cut(s) 39, 1041, 1235
StyD4I CCNGG 2 cut(s) 942, 961
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 5 cut(s) 131, 435, 509, 928, 1099
TaiI ACGT 2 cut(s) 30, 970
TaqI TCGA 7 cut(s) 183, 315, 632, 998, 1015, 1227, 1282
TasI AATT 8 cut(s) 92, 119, 205, 322, 422, 514, 582, 1223
TatI WGTACW 1 cut(s) 457
TauI GCSGC 1 cut(s) 1057
TfiI GAWTC 4 cut(s) 64, 167, 660, 893
Tru1I TTAA 4 cut(s) 107, 504, 735, 777
Tru9I TTAA 4 cut(s) 107, 504, 735, 777
TscAI CASTG 2 cut(s) 440, 931
TseFI GTSAC 4 cut(s) 51, 487, 568, 938
TseI GCWGC 3 cut(s) 33, 521, 1115
Tsp45I GTSAC 4 cut(s) 51, 487, 568, 938
TspDTI ATGAA 3 cut(s) 105, 652, 818
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 2 cut(s) 440, 931
Vha464I CTTAAG 1 cut(s) 734
VneI GTGCAC 1 cut(s) 577
VpaK11BI GGWCC 2 cut(s) 274, 333
XapI RAATTY 2 cut(s) 92, 119
XhoI CTCGAG 1 cut(s) 1014
XmiI GTMKAC 3 cut(s) 998, 1164, 1282
XspI CTAG 3 cut(s) 39, 1041, 1235
Zsp2I ATGCAT 2 cut(s) 190, 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.