Rroxscaffold_1G00063460

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009345
Physical Location & Seq
Reverse (-)
85335346 .. 85339003
3658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00063460.1

Sequence Viewer

Length: 1182 bp
ATGGAAAAAGAGCCTTTACTCCCCTACACATCAAGCCCAAGAAGAAAGCTCCCATCTTTAACAACCCTCTGGCCTTTACTGGAGCACGACGAAGTCCCCGTCCCCTTAACGCCCTCAGAGTTCAAAGACCGCATCATCTTCGGCTCCCCCGCCGCCGCTTCTTCTTCGCCGCAACAAGACTCGTCTCCTCTGGTTGACGCCCTCACTCTCTCTCTCAATTCTCCCAGACCTCCTTCTTCCTCTTCGCTTTTGGACGCCAGTCACGCCACCACAGCGCCACCGCAAGGAGACCCACAAACGCCGCAGGGCTGGCTCATTGACCCGAACTACTCATGGACCAATACCAACCTGCACCGGTCCAAGACCGCTCCGGCCATGGCGGTTTTGAACGAGGTGAACCGCCGGTCAGGGCCTAAGCCACAAGTGAGTTCACAGTCGATTGTTAGGCAAGCTGTGATTCTTCTTATTGCGTATTTGACTCTGGGGGTGATTATATATTGGTTTAACCGTGATCATTTTGCTGCGAGTGAGACTCACCCTGTTGTGGATGCTTTGTATTTTTGTATTGTGACAATGTGCACTATTGGGTACGGGGATATCACGCCTAAGACAACGGCTACGAAGCTGTTTTCGATAATGTTTGTGTTGGTGGGGTTCGGGTTTATTGATATTTTGCTTAGTGGGATGGTGGCATTTGTGCTTGATTTGCAAGAGAGTTATTTGCTGAGGAGTGTGAAGGGTGTGGGGGAGAAGAAGGAGTCGTATATAATAGATGTGAAGAAGGGGAGGATGAGGATTAGGATGAAGGTGGGATTAGCATTGGGGGTTGTGGTGCTTTGTATTGGTGTTGGTGCTATGGTTATGCATTATGTGGAGAAGCTCGGATGGTTGGATTCGTTTTATCTGTCGGTTATGTCTGTTACGACTGTGGGGTATGGTGACCGGGCGTTCCAGTCTATGGAGGGTCGAATCTTTGCTTCAATCTGGTTGCTTGTGTCCACACTCGCGGTTGCTAGAGCTTTTCTGTATTTGGCTGAGGCAAGGGTGGATAAACGGCATAGGAAGATGGCAAAGTGGGTGCTTGGCCAGGATATGACTGTTTCTGAGTTCCTTGCTGCTGACATTGACAATAATGGCTTTGTGAGTCAACCTTTTGCCATTAGTGACCATGATGTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0004788 GO:0005215 GO:0005216 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005887 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006996 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009108 GO:0009229 GO:0009314 GO:0009416 GO:0009507 GO:0009532 GO:0009533 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009657 GO:0009668 GO:0009705 GO:0009987 GO:0010027 GO:0010196 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0022607 GO:0022803 GO:0022838 GO:0022840 GO:0022841 GO:0022842 GO:0022857 GO:0022890 GO:0030001 GO:0030322 GO:0031090 GO:0031224 GO:0031226 GO:0031976 GO:0031984 GO:0034220 GO:0034641 GO:0042357 GO:0042391 GO:0042723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046483 GO:0046873 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051259 GO:0051260 GO:0055085 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0071704 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072527 GO:0072528 GO:0090407 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990066
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

393

Amino Acids

43.36

Weight (kDa)

7.73

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 152 - 226 2.7e-06 Ion transport protein
Ion_trans_2 PF07885 153 - 233 1.3e-16 Ion channel
Ion_trans_2 PF07885 277 - 346 1e-11 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 357
AccB7I CCANNNNNTGG 1 cut(s) 958
AccBSI CCGCTC 1 cut(s) 368
AccII CGCG 1 cut(s) 1007
AcoI YGGCCR 2 cut(s) 372, 1084
AcyI GRCGYC 2 cut(s) 198, 255
AfaI GTAC 1 cut(s) 590
AfiI CCNNNNNNNGG 3 cut(s) 284, 544, 958
AgeI ACCGGT 1 cut(s) 354
AgsI TTSAA 3 cut(s) 124, 388, 981
AjnI CCWGG 1 cut(s) 1086
AluBI AGCT 5 cut(s) 49, 452, 625, 880, 1019
AluI AGCT 5 cut(s) 49, 452, 625, 880, 1019
Alw21I GWGCWC 2 cut(s) 87, 581
Alw26I GTCTC 3 cut(s) 189, 282, 524
Alw44I GTGCAC 1 cut(s) 577
AoxI GGCC 4 cut(s) 71, 372, 410, 1084
ApaLI GTGCAC 1 cut(s) 577
ApeKI GCWGC 2 cut(s) 521, 1115
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AsiGI ACCGGT 1 cut(s) 354
AspLEI GCGC 1 cut(s) 277
AspS9I GGNCC 3 cut(s) 336, 357, 410
AsuC2I CCSGG 1 cut(s) 944
AsuHPI GGTGA 4 cut(s) 406, 499, 527, 950
AvaII GGWCC 2 cut(s) 336, 357
BaeGI GKGCMC 1 cut(s) 581
BalI TGGCCA 1 cut(s) 1086
Bbv12I GWGCWC 2 cut(s) 87, 581
BbvCI CCTCAGC 2 cut(s) 725, 1035
BbvI GCAGC 2 cut(s) 508, 1102
BccI CCATC 4 cut(s) 61, 679, 879, 1060
BceAI ACGGC 2 cut(s) 630, 1070
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BciT130I CCWGG 1 cut(s) 1088
BclI TGATCA 1 cut(s) 511
BcnI CCSGG 1 cut(s) 944
BcoDI GTCTC 3 cut(s) 189, 282, 524
BfaI CTAG 2 cut(s) 1014, 1180
BfoI RGCGCY 1 cut(s) 278
BfuAI ACCTGC 1 cut(s) 357
BisI GCNGC 6 cut(s) 153, 156, 170, 302, 522, 1116
BlsI GCNGC 6 cut(s) 154, 157, 171, 303, 523, 1117
Bme1390I CCNGG 2 cut(s) 944, 1088
Bme18I GGWCC 2 cut(s) 336, 357
BmgT120I GGNCC 3 cut(s) 336, 357, 410
BmiI GGNNCC 1 cut(s) 145
BmrFI CCNGG 2 cut(s) 944, 1088
BmsI GCATC 2 cut(s) 141, 538
BoxI GACNNNNGTC 1 cut(s) 258
BplI GAGNNNNNCTC 2 cut(s) 517, 549
BpmI CTGGAG 1 cut(s) 101
Bpu10I CCTNAGC 3 cut(s) 414, 725, 1035
BpuMI CCSGG 1 cut(s) 944
BsaHI GRCGYC 2 cut(s) 198, 255
BsaI GGTCTC 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 375
BsaWI WCCGGW 1 cut(s) 354
Bsc4I CCNNNNNNNGG 3 cut(s) 284, 544, 958
Bse118I RCCGGY 2 cut(s) 354, 402
Bse1I ACTGG 3 cut(s) 84, 258, 952
BseBI CCWGG 1 cut(s) 1088
BseDI CCNNGG 1 cut(s) 375
BseGI GGATG 5 cut(s) 553, 690, 795, 807, 890
BseLI CCNNNNNNNGG 3 cut(s) 284, 544, 958
BseMII CTCAG 4 cut(s) 129, 716, 1026, 1095
BseNI ACTGG 3 cut(s) 84, 258, 952
BseRI GAGGAG 2 cut(s) 177, 742
BseSI GKGCMC 1 cut(s) 581
BseXI GCAGC 2 cut(s) 508, 1102
BsgI GTGCAG 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 1007
BshFI GGCC 4 cut(s) 73, 374, 412, 1086
BshTI ACCGGT 1 cut(s) 354
BsiHKAI GWGCWC 2 cut(s) 87, 581
BsiSI CCGG 4 cut(s) 355, 371, 403, 943
BslFI GGGAC 2 cut(s) 80, 86
BslI CCNNNNNNNGG 3 cut(s) 284, 544, 958
BsmAI GTCTC 3 cut(s) 189, 282, 524
BsmBI CGTCTC 1 cut(s) 189
BsmFI GGGAC 2 cut(s) 80, 86
BsnI GGCC 4 cut(s) 73, 374, 412, 1086
Bso31I GGTCTC 1 cut(s) 282
Bsp1286I GDGCHC 2 cut(s) 87, 581
Bsp143I GATC 1 cut(s) 511
Bsp19I CCATGG 1 cut(s) 375
BspANI GGCC 4 cut(s) 73, 374, 412, 1086
BspCNI CTCAG 4 cut(s) 128, 717, 1027, 1096
BspFNI CGCG 1 cut(s) 1007
BspLI GGNNCC 1 cut(s) 145
BspMI ACCTGC 1 cut(s) 357
BspTNI GGTCTC 1 cut(s) 282
BsrBI CCGCTC 1 cut(s) 368
BsrFI RCCGGY 2 cut(s) 354, 402
BsrI ACTGG 3 cut(s) 84, 258, 952
BssAI RCCGGY 2 cut(s) 354, 402
BssECI CCNNGG 1 cut(s) 375
BssMI GATC 1 cut(s) 511
BssNI GRCGYC 2 cut(s) 198, 255
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 1088
Bst4CI ACNGT 4 cut(s) 435, 509, 928, 1099
Bst6I CTCTTC 1 cut(s) 247
BstACI GRCGYC 2 cut(s) 198, 255
BstC8I GCNNGC 2 cut(s) 311, 450
BstDEI CTNAG 7 cut(s) 115, 414, 606, 677, 725, 1035, 1104
BstDSI CCRYGG 1 cut(s) 375
BstEII GGTNACC 1 cut(s) 938
BstF5I GGATG 5 cut(s) 553, 690, 795, 807, 890
BstFNI CGCG 1 cut(s) 1007
BstH2I RGCGCY 1 cut(s) 278
BstHHI GCGC 1 cut(s) 277
BstKTI GATC 1 cut(s) 514
BstMAI GTCTC 3 cut(s) 189, 282, 524
BstMBI GATC 1 cut(s) 511
BstMWI GCNNNNNNNGC 4 cut(s) 263, 272, 310, 706
BstNI CCWGG 1 cut(s) 1088
BstPAI GACNNNNGTC 1 cut(s) 258
BstPI GGTNACC 1 cut(s) 938
BstSCI CCNGG 2 cut(s) 942, 1086
BstSLI GKGCMC 1 cut(s) 581
BstUI CGCG 1 cut(s) 1007
BstV1I GCAGC 2 cut(s) 508, 1102
BsuRI GGCC 4 cut(s) 73, 374, 412, 1086
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 5 cut(s) 553, 690, 795, 807, 890
BveI ACCTGC 1 cut(s) 357
Cac8I GCNNGC 2 cut(s) 311, 450
CfoI GCGC 1 cut(s) 277
Cfr10I RCCGGY 2 cut(s) 354, 402
Cfr13I GGNCC 3 cut(s) 336, 357, 410
CseI GACGC 2 cut(s) 206, 263
Csp6I GTAC 1 cut(s) 589
CspAI ACCGGT 1 cut(s) 354
CviAII CATG 3 cut(s) 333, 376, 1169
CviQI GTAC 1 cut(s) 589
DdeI CTNAG 7 cut(s) 115, 414, 606, 677, 725, 1035, 1104
DpnI GATC 1 cut(s) 513
DpnII GATC 1 cut(s) 511
EaeI YGGCCR 2 cut(s) 372, 1084
Eam1104I CTCTTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 247
Eco130I CCWWGG 1 cut(s) 375
Eco31I GGTCTC 1 cut(s) 282
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 2 cut(s) 336, 357
Eco91I GGTNACC 1 cut(s) 938
EcoO109I RGGNCCY 1 cut(s) 410
EcoO65I GGTNACC 1 cut(s) 938
EcoRII CCWGG 1 cut(s) 1086
EcoRV GATATC 1 cut(s) 598
EcoT14I CCWWGG 1 cut(s) 375
EcoT22I ATGCAT 1 cut(s) 867
ErhI CCWWGG 1 cut(s) 375
Esp3I CGTCTC 1 cut(s) 189
FaeI CATG 3 cut(s) 336, 379, 1172
FaqI GGGAC 2 cut(s) 80, 86
FatI CATG 3 cut(s) 332, 375, 1168
FauI CCCGC 1 cut(s) 157
FbaI TGATCA 1 cut(s) 511
Fnu4HI GCNGC 6 cut(s) 153, 156, 170, 302, 522, 1116
FokI GGATG 5 cut(s) 560, 697, 802, 814, 897
Fsp4HI GCNGC 6 cut(s) 153, 156, 170, 302, 522, 1116
FspBI CTAG 2 cut(s) 1014, 1180
GlaI GCGC 1 cut(s) 276
GluI GCNGC 6 cut(s) 153, 156, 170, 302, 522, 1116
GsuI CTGGAG 1 cut(s) 101
HaeII RGCGCY 1 cut(s) 278
HaeIII GGCC 4 cut(s) 73, 374, 412, 1086
HapII CCGG 4 cut(s) 355, 371, 403, 943
HgaI GACGC 2 cut(s) 206, 263
HhaI GCGC 1 cut(s) 277
Hin1I GRCGYC 2 cut(s) 198, 255
Hin1II CATG 3 cut(s) 336, 379, 1172
Hin6I GCGC 1 cut(s) 275
HinP1I GCGC 1 cut(s) 275
HincII GTYRAC 2 cut(s) 196, 1148
HindII GTYRAC 2 cut(s) 196, 1148
HinfI GANTC 8 cut(s) 179, 457, 478, 532, 758, 893, 969, 1144
HpaII CCGG 4 cut(s) 355, 371, 403, 943
HphI GGTGA 4 cut(s) 406, 499, 527, 950
Hpy166II GTNNAC 6 cut(s) 196, 397, 431, 579, 999, 1148
Hpy188I TCNGA 3 cut(s) 118, 884, 1105
Hpy8I GTNNAC 6 cut(s) 196, 397, 431, 579, 999, 1148
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 5 cut(s) 243, 730, 748, 775, 799
HpyCH4III ACNGT 4 cut(s) 435, 509, 928, 1099
HpyCH4V TGCA 4 cut(s) 352, 579, 709, 865
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 272, 310, 706
HpyF3I CTNAG 7 cut(s) 115, 414, 606, 677, 725, 1035, 1104
Hsp92I GRCGYC 2 cut(s) 198, 255
Hsp92II CATG 3 cut(s) 336, 379, 1172
HspAI GCGC 1 cut(s) 275
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 1 cut(s) 511
LmnI GCTCC 4 cut(s) 54, 82, 149, 373
Lsp1109I GCAGC 2 cut(s) 508, 1102
LweI GCATC 2 cut(s) 141, 538
MaeI CTAG 2 cut(s) 1014, 1180
MaeIII GTNAC 5 cut(s) 260, 568, 919, 938, 1163
MalI GATC 1 cut(s) 513
MbiI CCGCTC 1 cut(s) 368
MboI GATC 1 cut(s) 511
MhlI GDGCHC 2 cut(s) 87, 581
MlsI TGGCCA 1 cut(s) 1086
MluCI AATT 1 cut(s) 217
MluNI TGGCCA 1 cut(s) 1086
MlyI GAGTC 5 cut(s) 173, 472, 526, 767, 1153
MmeI TCCRAC 1 cut(s) 870
Mox20I TGGCCA 1 cut(s) 1086
Mph1103I ATGCAT 1 cut(s) 867
MscI TGGCCA 1 cut(s) 1086
MseI TTAA 3 cut(s) 59, 107, 504
Msp20I TGGCCA 1 cut(s) 1086
MspI CCGG 4 cut(s) 355, 371, 403, 943
MspR9I CCNGG 2 cut(s) 944, 1088
MvaI CCWGG 1 cut(s) 1088
MvnI CGCG 1 cut(s) 1007
MwoI GCNNNNNNNGC 4 cut(s) 263, 272, 310, 706
NciI CCSGG 1 cut(s) 944
NcoI CCATGG 1 cut(s) 375
NdeII GATC 1 cut(s) 511
NlaIII CATG 3 cut(s) 336, 379, 1172
NlaIV GGNNCC 1 cut(s) 145
NmuCI GTSAC 4 cut(s) 260, 568, 938, 1163
NsiI ATGCAT 1 cut(s) 867
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 3 cut(s) 457, 893, 969
PflFI GACNNNGTC 1 cut(s) 92
PflMI CCANNNNNTGG 1 cut(s) 958
PinAI ACCGGT 1 cut(s) 354
PkrI GCNGC 6 cut(s) 154, 157, 171, 303, 523, 1117
PleI GAGTC 5 cut(s) 173, 472, 526, 766, 1152
PpsI GAGTC 5 cut(s) 173, 472, 526, 766, 1152
PshAI GACNNNNGTC 1 cut(s) 258
Psp6I CCWGG 1 cut(s) 1086
PspEI GGTNACC 1 cut(s) 938
PspGI CCWGG 1 cut(s) 1086
PspN4I GGNNCC 1 cut(s) 145
PspPI GGNCC 3 cut(s) 336, 357, 410
PsyI GACNNNGTC 1 cut(s) 92
RsaI GTAC 1 cut(s) 590
RsaNI GTAC 1 cut(s) 589
SaqAI TTAA 3 cut(s) 59, 107, 504
SatI GCNGC 6 cut(s) 153, 156, 170, 302, 522, 1116
Sau3AI GATC 1 cut(s) 511
Sau96I GGNCC 3 cut(s) 336, 357, 410
SchI GAGTC 5 cut(s) 173, 472, 526, 767, 1153
ScrFI CCNGG 2 cut(s) 944, 1088
SduI GDGCHC 2 cut(s) 87, 581
SfaNI GCATC 2 cut(s) 141, 538
SinI GGWCC 2 cut(s) 336, 357
Sse9I AATT 1 cut(s) 217
SspMI CTAG 2 cut(s) 1014, 1180
StyD4I CCNGG 2 cut(s) 942, 1086
StyI CCWWGG 1 cut(s) 375
TaaI ACNGT 4 cut(s) 435, 509, 928, 1099
TaqI TCGA 3 cut(s) 437, 632, 967
TasI AATT 1 cut(s) 217
TauI GCSGC 4 cut(s) 155, 158, 172, 304
TfiI GAWTC 3 cut(s) 457, 893, 969
Tru1I TTAA 3 cut(s) 59, 107, 504
Tru9I TTAA 3 cut(s) 59, 107, 504
TseFI GTSAC 4 cut(s) 260, 568, 938, 1163
TseI GCWGC 2 cut(s) 521, 1115
Tsp45I GTSAC 4 cut(s) 260, 568, 938, 1163
TspDTI ATGAA 1 cut(s) 818
Tth111I GACNNNGTC 1 cut(s) 92
Van91I CCANNNNNTGG 1 cut(s) 958
VneI GTGCAC 1 cut(s) 577
VpaK11BI GGWCC 2 cut(s) 336, 357
XspI CTAG 2 cut(s) 1014, 1180
Zsp2I ATGCAT 1 cut(s) 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.