Rh5AG101500

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0009345
Physical Location & Seq
Forward (+)
9402582 .. 9406516
3935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG101500.1

Sequence Viewer

Length: 1281 bp
ATGGACAAAGAGCCTTTACTCCCCTACACATCAAGCCCAAGAAGAAAGCTCCCATCTTTAACAACCCTCTGGCCTCTACCGGAGCACGACGAAGTCCCCGTCCCCTTGACCCCCTCAGAGTTCAAGGACCGCATCATCTTCGGCTCCCCCGCCGCCGCTTCTTCTTCGCCGCAACAAGACTCGTCTCCTCTGGTTGACGCCCTCACTCTCTCTCTCAATTCTCCCAGACCTCCTTCTTCCGCTTCGCTGTTGGATCCCACCACAGCGCCGCCGCAAGGGGACCCACAAACGCCGCAGGGCTGGCTCATTGACCCGAACTACTCATGGACCAATACCAACCTGCACCGGTCCAAGACCGCTCCGGCCATGGCGGTTTTGAACGAGGTGAACCGCCGGTCAGGGCCTAAGCCACAAGTGAGTTCACAGTCGATTGTTAGGCAAGCGGTGATTCTTCTTATTGCGTATTTGACTCTGGGGGTGATTATATATTGGTTTAACCGTGATCATTTTGCTGCGAGTGAGACTCACCCTGTTGTGGATGCTTTGTATTTTTGTATTGTGACAATGTGCACTATTGGGTATGGGGATATCACGCCTAAGACCACGGCTACGAAGCTGTTTTCGATAATGTTTGTGTTGGTGGGGTTCGGGTTTATTGACATTTTACTTAGTGGGATGGTGGCTTATGTGCTTGATTTGCAAGAGAGTTATTTGCTGAGGAGTGTGAAGGGGGTGGGGGAGAAGAAGGAGTCGTATATAATTGATGTGAAGAAGGGGAGGATGAGGATCAGGATGAAGGTGGGATTGGCATTGGGGGTTGTGGTGCTTTGTATTGGTGTTGGTGCTATGGTTATGCATTATGTGGAGAAGCTCGGATGGTTGGATTCGTTTTATCTGTCGGTGATGTCTGTTACGACTGTGGGGTATGGTGACCGGGCGTTCCAGTCTATGGAGGGTCGAATCTTTGCATCAATCTGGTTGCTTGTGTCCACACTCGCGGTTGCTAGAGCCTTTCTGTATTTGGCTGAGGCAAGGGTGGATAAACGGCATAGGAAGATGGCAAAGTGGGTTCTTGGCCAGGATATGACTGTTTCTGAGTTCCTTGCTGCTGACATTGACAATAATGGCTTTGTGAGCAAGTCAGAGTATGTTGTATACAAACTCAAGGAGATGGGAAAGGTATCAGAGAAAGATATTATGCAGATCTCCAACAAATTTGATAGGCTAGACGCTGGGAACTGCGGAAAGATAACCCTCGCTGATCTTATGAGTCGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0004788 GO:0005215 GO:0005216 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005887 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006996 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009108 GO:0009229 GO:0009314 GO:0009416 GO:0009507 GO:0009532 GO:0009533 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009657 GO:0009668 GO:0009705 GO:0009987 GO:0010027 GO:0010196 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0022607 GO:0022803 GO:0022838 GO:0022840 GO:0022841 GO:0022842 GO:0022857 GO:0022890 GO:0030001 GO:0030322 GO:0031090 GO:0031224 GO:0031226 GO:0031976 GO:0031984 GO:0034220 GO:0034641 GO:0042357 GO:0042391 GO:0042723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046483 GO:0046873 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051259 GO:0051260 GO:0055085 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0071704 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072527 GO:0072528 GO:0090407 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990066
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

426

Amino Acids

47.22

Weight (kDa)

8.82

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 149 - 227 1.5e-06 Ion transport protein
Ion_trans_2 PF07885 150 - 229 1.7e-16 Ion channel
Ion_trans_2 PF07885 274 - 343 1.1e-11 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 348
AccB7I CCANNNNNTGG 1 cut(s) 949
AccBSI CCGCTC 1 cut(s) 359
AccI GTMKAC 1 cut(s) 1155
AccII CGCG 1 cut(s) 998
AclWI GGATC 3 cut(s) 248, 261, 794
AcoI YGGCCR 2 cut(s) 363, 1075
AcsI RAATTY 1 cut(s) 1214
AcyI GRCGYC 1 cut(s) 198
AfiI CCNNNNNNNGG 3 cut(s) 275, 535, 949
AgeI ACCGGT 1 cut(s) 345
AgsI TTSAA 2 cut(s) 124, 379
AjnI CCWGG 1 cut(s) 1077
AjuI GAANNNNNNNTTGG 2 cut(s) 788, 820
AluBI AGCT 3 cut(s) 49, 616, 871
AluI AGCT 3 cut(s) 49, 616, 871
Alw21I GWGCWC 2 cut(s) 87, 572
Alw26I GTCTC 2 cut(s) 189, 515
Alw44I GTGCAC 1 cut(s) 568
AlwI GGATC 3 cut(s) 248, 261, 794
AoxI GGCC 4 cut(s) 71, 363, 401, 1075
ApaLI GTGCAC 1 cut(s) 568
ApeKI GCWGC 2 cut(s) 512, 1106
ApoI RAATTY 1 cut(s) 1214
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AsiGI ACCGGT 1 cut(s) 345
AspLEI GCGC 1 cut(s) 268
AspS9I GGNCC 5 cut(s) 127, 280, 327, 348, 401
AsuC2I CCSGG 1 cut(s) 935
AsuHPI GGTGA 6 cut(s) 397, 457, 490, 518, 913, 941
AvaII GGWCC 4 cut(s) 127, 280, 327, 348
BaeGI GKGCMC 1 cut(s) 572
BalI TGGCCA 1 cut(s) 1077
BamHI GGATCC 1 cut(s) 253
Bbv12I GWGCWC 2 cut(s) 87, 572
BbvCI CCTCAGC 2 cut(s) 716, 1026
BbvI GCAGC 2 cut(s) 499, 1093
BccI CCATC 5 cut(s) 61, 670, 870, 1051, 1165
BceAI ACGGC 2 cut(s) 621, 1061
BcgI CGANNNNNNTGC 2 cut(s) 121, 155
BciT130I CCWGG 1 cut(s) 1079
BclI TGATCA 1 cut(s) 502
BcnI CCSGG 1 cut(s) 935
BcoDI GTCTC 2 cut(s) 189, 515
BfaI CTAG 2 cut(s) 1005, 1226
BfoI RGCGCY 1 cut(s) 269
BfuAI ACCTGC 1 cut(s) 348
BglII AGATCT 1 cut(s) 1203
BisI GCNGC 8 cut(s) 153, 156, 170, 269, 272, 293, 513, 1107
BlsI GCNGC 8 cut(s) 154, 157, 171, 270, 273, 294, 514, 1108
Bme1390I CCNGG 2 cut(s) 935, 1079
Bme18I GGWCC 4 cut(s) 127, 280, 327, 348
BmgT120I GGNCC 5 cut(s) 127, 280, 327, 348, 401
BmiI GGNNCC 4 cut(s) 145, 255, 281, 282
BmrFI CCNGG 2 cut(s) 935, 1079
BmsI GCATC 3 cut(s) 141, 529, 977
BplI GAGNNNNNCTC 2 cut(s) 508, 540
Bpu10I CCTNAGC 3 cut(s) 405, 716, 1026
BpuEI CTTGAG 1 cut(s) 1148
BpuMI CCSGG 1 cut(s) 935
BsaBI GATNNNNATC 1 cut(s) 785
BsaHI GRCGYC 1 cut(s) 198
BsaJI CCNNGG 2 cut(s) 366, 603
BsaWI WCCGGW 2 cut(s) 79, 345
Bsc4I CCNNNNNNNGG 3 cut(s) 275, 535, 949
Bse118I RCCGGY 2 cut(s) 345, 393
Bse1I ACTGG 1 cut(s) 943
Bse8I GATNNNNATC 1 cut(s) 785
BseBI CCWGG 1 cut(s) 1079
BseDI CCNNGG 2 cut(s) 366, 603
BseGI GGATG 5 cut(s) 544, 681, 786, 798, 881
BseJI GATNNNNATC 1 cut(s) 785
BseLI CCNNNNNNNGG 3 cut(s) 275, 535, 949
BseMII CTCAG 4 cut(s) 129, 707, 1017, 1086
BseNI ACTGG 1 cut(s) 943
BseRI GAGGAG 2 cut(s) 177, 733
BseSI GKGCMC 1 cut(s) 572
BseXI GCAGC 2 cut(s) 499, 1093
BseYI CCCAGC 1 cut(s) 1232
BsgI GTGCAG 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 998
BshFI GGCC 4 cut(s) 73, 365, 403, 1077
BshTI ACCGGT 1 cut(s) 345
BsiHKAI GWGCWC 2 cut(s) 87, 572
BsiSI CCGG 5 cut(s) 80, 346, 362, 394, 934
BslFI GGGAC 3 cut(s) 80, 86, 293
BslI CCNNNNNNNGG 3 cut(s) 275, 535, 949
BsmAI GTCTC 2 cut(s) 189, 515
BsmBI CGTCTC 1 cut(s) 189
BsmFI GGGAC 3 cut(s) 80, 86, 293
BsnI GGCC 4 cut(s) 73, 365, 403, 1077
Bsp1286I GDGCHC 2 cut(s) 87, 572
Bsp143I GATC 5 cut(s) 253, 502, 786, 1203, 1261
Bsp19I CCATGG 1 cut(s) 366
BspANI GGCC 4 cut(s) 73, 365, 403, 1077
BspCNI CTCAG 4 cut(s) 128, 708, 1018, 1087
BspFNI CGCG 1 cut(s) 998
BspLI GGNNCC 4 cut(s) 145, 255, 281, 282
BspMI ACCTGC 1 cut(s) 348
BspPI GGATC 3 cut(s) 248, 261, 794
BsrBI CCGCTC 1 cut(s) 359
BsrFI RCCGGY 2 cut(s) 345, 393
BsrI ACTGG 1 cut(s) 943
BssAI RCCGGY 2 cut(s) 345, 393
BssECI CCNNGG 2 cut(s) 366, 603
BssMI GATC 5 cut(s) 253, 502, 786, 1203, 1261
BssNAI GTATAC 1 cut(s) 1156
BssNI GRCGYC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 366
Bst1107I GTATAC 1 cut(s) 1156
Bst2UI CCWGG 1 cut(s) 1079
Bst4CI ACNGT 4 cut(s) 426, 500, 919, 1090
BstACI GRCGYC 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 302, 441
BstDEI CTNAG 7 cut(s) 115, 405, 597, 668, 716, 1026, 1095
BstDSI CCRYGG 2 cut(s) 366, 603
BstEII GGTNACC 1 cut(s) 929
BstF5I GGATG 5 cut(s) 544, 681, 786, 798, 881
BstFNI CGCG 1 cut(s) 998
BstH2I RGCGCY 1 cut(s) 269
BstHHI GCGC 1 cut(s) 268
BstKTI GATC 5 cut(s) 256, 505, 789, 1206, 1264
BstMAI GTCTC 2 cut(s) 189, 515
BstMBI GATC 5 cut(s) 253, 502, 786, 1203, 1261
BstMWI GCNNNNNNNGC 3 cut(s) 301, 697, 1134
BstNI CCWGG 1 cut(s) 1079
BstPI GGTNACC 1 cut(s) 929
BstSCI CCNGG 2 cut(s) 933, 1077
BstSLI GKGCMC 1 cut(s) 572
BstUI CGCG 1 cut(s) 998
BstV1I GCAGC 2 cut(s) 499, 1093
BstX2I RGATCY 2 cut(s) 253, 1203
BstYI RGATCY 2 cut(s) 253, 1203
BstZ17I GTATAC 1 cut(s) 1156
BsuRI GGCC 4 cut(s) 73, 365, 403, 1077
BtgI CCRYGG 2 cut(s) 366, 603
BtsCI GGATG 5 cut(s) 544, 681, 786, 798, 881
BveI ACCTGC 1 cut(s) 348
Cac8I GCNNGC 2 cut(s) 302, 441
CfoI GCGC 1 cut(s) 268
Cfr10I RCCGGY 2 cut(s) 345, 393
Cfr13I GGNCC 5 cut(s) 127, 280, 327, 348, 401
CseI GACGC 2 cut(s) 206, 1238
CspAI ACCGGT 1 cut(s) 345
CviAII CATG 2 cut(s) 324, 367
DdeI CTNAG 7 cut(s) 115, 405, 597, 668, 716, 1026, 1095
DpnI GATC 5 cut(s) 255, 504, 788, 1205, 1263
DpnII GATC 5 cut(s) 253, 502, 786, 1203, 1261
EaeI YGGCCR 2 cut(s) 363, 1075
Eco130I CCWWGG 1 cut(s) 366
Eco32I GATATC 1 cut(s) 589
Eco47I GGWCC 4 cut(s) 127, 280, 327, 348
Eco91I GGTNACC 1 cut(s) 929
EcoO109I RGGNCCY 2 cut(s) 280, 401
EcoO65I GGTNACC 1 cut(s) 929
EcoRII CCWGG 1 cut(s) 1077
EcoRV GATATC 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 366
EcoT22I ATGCAT 1 cut(s) 858
ErhI CCWWGG 1 cut(s) 366
Esp3I CGTCTC 1 cut(s) 189
FaeI CATG 2 cut(s) 327, 370
FaqI GGGAC 3 cut(s) 80, 86, 293
FatI CATG 2 cut(s) 323, 366
FauI CCCGC 1 cut(s) 157
FbaI TGATCA 1 cut(s) 502
FblI GTMKAC 1 cut(s) 1155
Fnu4HI GCNGC 8 cut(s) 153, 156, 170, 269, 272, 293, 513, 1107
FokI GGATG 5 cut(s) 551, 688, 793, 805, 888
Fsp4HI GCNGC 8 cut(s) 153, 156, 170, 269, 272, 293, 513, 1107
FspBI CTAG 2 cut(s) 1005, 1226
GlaI GCGC 1 cut(s) 267
GluI GCNGC 8 cut(s) 153, 156, 170, 269, 272, 293, 513, 1107
GsaI CCCAGC 1 cut(s) 1236
HaeII RGCGCY 1 cut(s) 269
HaeIII GGCC 4 cut(s) 73, 365, 403, 1077
HapII CCGG 5 cut(s) 80, 346, 362, 394, 934
HgaI GACGC 2 cut(s) 206, 1238
HhaI GCGC 1 cut(s) 268
Hin1I GRCGYC 1 cut(s) 198
Hin1II CATG 2 cut(s) 327, 370
Hin6I GCGC 1 cut(s) 266
HinP1I GCGC 1 cut(s) 266
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 8 cut(s) 179, 448, 469, 523, 749, 884, 960, 1270
HpaII CCGG 5 cut(s) 80, 346, 362, 394, 934
HphI GGTGA 6 cut(s) 397, 457, 490, 518, 913, 941
Hpy166II GTNNAC 6 cut(s) 196, 388, 422, 570, 990, 1156
Hpy188I TCNGA 5 cut(s) 118, 875, 1096, 1144, 1186
Hpy188III TCNNGA 1 cut(s) 790
Hpy8I GTNNAC 6 cut(s) 196, 388, 422, 570, 990, 1156
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 5 cut(s) 243, 721, 739, 766, 790
HpyCH4III ACNGT 4 cut(s) 426, 500, 919, 1090
HpyCH4V TGCA 6 cut(s) 343, 570, 700, 856, 968, 1201
HpyF10VI GCNNNNNNNGC 3 cut(s) 301, 697, 1134
HpyF3I CTNAG 7 cut(s) 115, 405, 597, 668, 716, 1026, 1095
Hsp92I GRCGYC 1 cut(s) 198
Hsp92II CATG 2 cut(s) 327, 370
HspAI GCGC 1 cut(s) 266
KflI GGGWCCC 1 cut(s) 280
Ksp22I TGATCA 1 cut(s) 502
Kzo9I GATC 5 cut(s) 253, 502, 786, 1203, 1261
LmnI GCTCC 4 cut(s) 54, 82, 149, 364
Lsp1109I GCAGC 2 cut(s) 499, 1093
LweI GCATC 3 cut(s) 141, 529, 977
MaeI CTAG 2 cut(s) 1005, 1226
MaeIII GTNAC 3 cut(s) 559, 910, 929
MalI GATC 5 cut(s) 255, 504, 788, 1205, 1263
MbiI CCGCTC 1 cut(s) 359
MboI GATC 5 cut(s) 253, 502, 786, 1203, 1261
MboII GAAGA 9 cut(s) 54, 130, 153, 156, 228, 443, 754, 781, 1066
MflI RGATCY 2 cut(s) 253, 1203
MhlI GDGCHC 2 cut(s) 87, 572
MlsI TGGCCA 1 cut(s) 1077
MluCI AATT 3 cut(s) 217, 759, 1214
MluNI TGGCCA 1 cut(s) 1077
MlyI GAGTC 5 cut(s) 173, 463, 517, 758, 1279
MmeI TCCRAC 3 cut(s) 231, 861, 1233
Mox20I TGGCCA 1 cut(s) 1077
Mph1103I ATGCAT 1 cut(s) 858
MscI TGGCCA 1 cut(s) 1077
MseI TTAA 2 cut(s) 59, 495
Msp20I TGGCCA 1 cut(s) 1077
MspI CCGG 5 cut(s) 80, 346, 362, 394, 934
MspR9I CCNGG 2 cut(s) 935, 1079
MvaI CCWGG 1 cut(s) 1079
MvnI CGCG 1 cut(s) 998
MwoI GCNNNNNNNGC 3 cut(s) 301, 697, 1134
NciI CCSGG 1 cut(s) 935
NcoI CCATGG 1 cut(s) 366
NdeII GATC 5 cut(s) 253, 502, 786, 1203, 1261
NlaIII CATG 2 cut(s) 327, 370
NlaIV GGNNCC 4 cut(s) 145, 255, 281, 282
NmuCI GTSAC 2 cut(s) 559, 929
NsiI ATGCAT 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 3 cut(s) 448, 884, 960
PflFI GACNNNGTC 1 cut(s) 92
PflMI CCANNNNNTGG 1 cut(s) 949
PinAI ACCGGT 1 cut(s) 345
PkrI GCNGC 8 cut(s) 154, 157, 171, 270, 273, 294, 514, 1108
PleI GAGTC 5 cut(s) 173, 463, 517, 757, 1278
PpsI GAGTC 5 cut(s) 173, 463, 517, 757, 1278
PpuMI RGGWCCY 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 1077
PspEI GGTNACC 1 cut(s) 929
PspFI CCCAGC 1 cut(s) 1232
PspGI CCWGG 1 cut(s) 1077
PspN4I GGNNCC 4 cut(s) 145, 255, 281, 282
PspPI GGNCC 5 cut(s) 127, 280, 327, 348, 401
PspPPI RGGWCCY 1 cut(s) 280
PsuI RGATCY 2 cut(s) 253, 1203
PsyI GACNNNGTC 1 cut(s) 92
SaqAI TTAA 2 cut(s) 59, 495
SatI GCNGC 8 cut(s) 153, 156, 170, 269, 272, 293, 513, 1107
Sau3AI GATC 5 cut(s) 253, 502, 786, 1203, 1261
Sau96I GGNCC 5 cut(s) 127, 280, 327, 348, 401
SchI GAGTC 5 cut(s) 173, 463, 517, 758, 1279
ScrFI CCNGG 2 cut(s) 935, 1079
SduI GDGCHC 2 cut(s) 87, 572
SetI ASST 8 cut(s) 51, 232, 342, 387, 618, 801, 873, 1182
SfaNI GCATC 3 cut(s) 141, 529, 977
SinI GGWCC 4 cut(s) 127, 280, 327, 348
SmlI CTYRAG 1 cut(s) 1163
SmoI CTYRAG 1 cut(s) 1163
Sse9I AATT 3 cut(s) 217, 759, 1214
SspMI CTAG 2 cut(s) 1005, 1226
StyD4I CCNGG 2 cut(s) 933, 1077
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 4 cut(s) 426, 500, 919, 1090
TaqI TCGA 3 cut(s) 428, 623, 958
TasI AATT 3 cut(s) 217, 759, 1214
TauI GCSGC 6 cut(s) 155, 158, 172, 271, 274, 295
TfiI GAWTC 3 cut(s) 448, 884, 960
Tru1I TTAA 2 cut(s) 59, 495
Tru9I TTAA 2 cut(s) 59, 495
TseFI GTSAC 2 cut(s) 559, 929
TseI GCWGC 2 cut(s) 512, 1106
Tsp45I GTSAC 2 cut(s) 559, 929
TspDTI ATGAA 1 cut(s) 809
Tth111I GACNNNGTC 1 cut(s) 92
Van91I CCANNNNNTGG 1 cut(s) 949
VneI GTGCAC 1 cut(s) 568
VpaK11BI GGWCC 4 cut(s) 127, 280, 327, 348
XapI RAATTY 1 cut(s) 1214
XmiI GTMKAC 1 cut(s) 1155
XspI CTAG 2 cut(s) 1005, 1226
Zsp2I ATGCAT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.