FvH4_3g06210

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009345
Physical Location & Seq
Reverse (-)
3569501 .. 3574606
5106 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06210.t1

Sequence Viewer

Length: 1302 bp
ATGGACAAAGAGCCTCTACTCCCCTACACATCAAGCCCACGAAGAAAGCTCCCATCTTTAACCACCCTCTGGCCTTTGCCGGAACACGACGAAGTCCCCGTCCCCTTAACCCCGTTGGAGTTTAAAGACCGCATCATCTTCGGCTCCCCCGCCGCCGCCACCGCCACTTCTTCTTCGCCGCATGACTCGTCTCCTCTGGTCGACGCCCTCACTCTCTCTCTCAATTCTCCAAGACTCTCTTCGAGGCCCACATCCTCGTCTTCTCTACTGGACCCCACCACCGCGCCGCAGCCGCAGGGGGACCCACAAACGCCGCTGAGCTGGCTGATCGACCCGAACTACTCATGGACCAAGACCAACCTCCACAGGTCCAAGACGGCTCCGGCAATGGCGGTTTTGAACGAGGTGAACCGCCGGGCCGGGCCTAAGCCTCAGATGAGTTCACAGTCTATTGTGAGGCAAGCTGTGATTCTTCTTATTGCGTATTTGACATTAGGGGTGACTATATATTGGTTTAACCGTGATAATTTCGAAGCGAGTGAGACTCACCCTGTTGTGGATGCTCTGTATTTTTGCATTGTGACAATGTGCACTATTGGGTATGGGGATATCACACCCAAGACTACGGCTACCAAGCTGTTTTCAATAATGTTTGTGTTGGTGGGGTTCGGGTTTATTGATATATTGCTTAGTGGGATGGTGTCATATGTGCTTGATTTGCAGGAGGGTTATTTGCTGAGGAGTGTGAAGGGTGTGGGGGAGAAGAAGGAGTCGTATATTATTGATGTGAAGAAGGGGAGGATGAGGATTCGGATGAAGGTGGGACTGGCATTGGGGGTTGTGGTGCTTTGTATTGGTGTTGGTGCTATGGTGATGCATTATGTGGAGAAGTTAGGATGGTTGGATTCGTTTTATCTCTCGGTTATGTCTGTAACGACTGTGGGGTATGGTGACCGGGCTTTTCAGTCTGTGCAGGGTCGGTTGTTTGCTTCAGTTTGGTTGCTTGTGTCCACACTCACGGTTGCTAGAGCCTTTTTGTATTTGGCTGAGGCAAGGGTGGATAAACGGCATAGGAGGATGGCAAAGTGGGTTCTTGGCCAGGATATGACTGTTTCCGAGTTCCTTGCTGCTGACATTGACAATAATGGGTTTGTGAGCAAGTCAGAATATGTGATATACAAACTCAAGGAGATGGGAAAGGTATCAGAGAAAGATATTATGCAGATCTCCAACAAATTTGATAGGTTAGACTCTGGGAACTGCGGAAAGATAACGCTTGCCGATCTTATGAGTCGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0003824 GO:0004788 GO:0005215 GO:0005216 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0005887 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006772 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006996 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009108 GO:0009229 GO:0009314 GO:0009416 GO:0009507 GO:0009532 GO:0009533 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0009628 GO:0009657 GO:0009668 GO:0009705 GO:0009987 GO:0010027 GO:0010196 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0016740 GO:0016772 GO:0016778 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0022607 GO:0022803 GO:0022838 GO:0022840 GO:0022841 GO:0022842 GO:0022857 GO:0022890 GO:0030001 GO:0030322 GO:0031090 GO:0031224 GO:0031226 GO:0031976 GO:0031984 GO:0034220 GO:0034641 GO:0042357 GO:0042391 GO:0042723 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046483 GO:0046873 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051234 GO:0051259 GO:0051260 GO:0055085 GO:0061024 GO:0065003 GO:0065007 GO:0065008 GO:0071704 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0072527 GO:0072528 GO:0090407 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1990066
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

434

Amino Acids

48.09

Weight (kDa)

9.04

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 156 - 235 3e-07 Ion transport protein
Ion_trans_2 PF07885 157 - 237 1.2e-16 Ion channel
Ion_trans_2 PF07885 281 - 350 4.1e-12 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 69
AccI GTMKAC 1 cut(s) 201
AccII CGCG 1 cut(s) 284
AcoI YGGCCR 1 cut(s) 1096
AcsI RAATTY 1 cut(s) 1235
AcuI CTGAAG 1 cut(s) 975
AcyI GRCGYC 1 cut(s) 204
AfiI CCNNNNNNNGG 2 cut(s) 69, 556
AgsI TTSAA 2 cut(s) 400, 645
AjnI CCWGG 1 cut(s) 1098
AjuI GAANNNNNNNTTGG 2 cut(s) 223, 255
AluBI AGCT 4 cut(s) 49, 321, 464, 637
AluI AGCT 4 cut(s) 49, 321, 464, 637
Alw21I GWGCWC 1 cut(s) 593
Alw26I GTCTC 2 cut(s) 195, 536
Alw44I GTGCAC 1 cut(s) 589
AoxI GGCC 5 cut(s) 71, 245, 417, 422, 1096
ApaLI GTGCAC 1 cut(s) 589
ApeKI GCWGC 2 cut(s) 289, 1127
ApoI RAATTY 1 cut(s) 1235
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 7 cut(s) 246, 271, 301, 348, 369, 417, 422
AsuC2I CCSGG 3 cut(s) 416, 421, 956
AsuHPI GGTGA 5 cut(s) 418, 511, 539, 883, 962
AsuII TTCGAA 1 cut(s) 531
AvaII GGWCC 4 cut(s) 271, 301, 348, 369
BaeGI GKGCMC 1 cut(s) 593
BalI TGGCCA 1 cut(s) 1098
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 593
BbvCI CCTCAGC 2 cut(s) 737, 1047
BbvI GCAGC 2 cut(s) 301, 1114
BccI CCATC 5 cut(s) 61, 691, 891, 1072, 1186
BceAI ACGGC 3 cut(s) 393, 642, 1082
BcgI CGANNNNNNTGC 4 cut(s) 121, 155, 1106, 1140
BciT130I CCWGG 1 cut(s) 1100
BcnI CCSGG 3 cut(s) 416, 421, 956
BcoDI GTCTC 2 cut(s) 195, 536
BfaI CTAG 1 cut(s) 1026
BglII AGATCT 1 cut(s) 1224
BisI GCNGC 8 cut(s) 153, 156, 179, 287, 290, 293, 314, 1128
BlpI GCTNAGC 1 cut(s) 317
BlsI GCNGC 8 cut(s) 154, 157, 180, 288, 291, 294, 315, 1129
Bme1390I CCNGG 4 cut(s) 416, 421, 956, 1100
Bme18I GGWCC 4 cut(s) 271, 301, 348, 369
BmgT120I GGNCC 7 cut(s) 246, 271, 301, 348, 369, 417, 422
BmiI GGNNCC 5 cut(s) 145, 273, 302, 303, 381
BmrFI CCNGG 4 cut(s) 416, 421, 956, 1100
BmsI GCATC 3 cut(s) 141, 550, 864
BpiI GAAGAC 1 cut(s) 252
BplI GAGNNNNNCTC 2 cut(s) 529, 561
Bpu10I CCTNAGC 3 cut(s) 426, 737, 1047
Bpu1102I GCTNAGC 1 cut(s) 317
Bpu14I TTCGAA 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 1169
BpuMI CCSGG 3 cut(s) 416, 421, 956
BsaHI GRCGYC 1 cut(s) 204
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 556
Bse1I ACTGG 2 cut(s) 273, 831
Bse3DI GCAATG 1 cut(s) 393
BseBI CCWGG 1 cut(s) 1100
BseGI GGATG 7 cut(s) 251, 565, 702, 807, 819, 902, 1083
BseLI CCNNNNNNNGG 2 cut(s) 69, 556
BseMI GCAATG 1 cut(s) 393
BseMII CTCAG 4 cut(s) 308, 446, 728, 1038
BseNI ACTGG 2 cut(s) 273, 831
BseRI GAGGAG 2 cut(s) 183, 754
BseSI GKGCMC 1 cut(s) 593
BseXI GCAGC 2 cut(s) 301, 1114
BsgI GTGCAG 1 cut(s) 992
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 5 cut(s) 73, 247, 419, 424, 1098
BsiHKAI GWGCWC 1 cut(s) 593
BsiSI CCGG 5 cut(s) 80, 383, 415, 420, 955
BslFI GGGAC 4 cut(s) 80, 86, 314, 837
BslI CCNNNNNNNGG 2 cut(s) 69, 556
BsmAI GTCTC 2 cut(s) 195, 536
BsmBI CGTCTC 1 cut(s) 195
BsmFI GGGAC 4 cut(s) 80, 86, 314, 837
BsnI GGCC 5 cut(s) 73, 247, 419, 424, 1098
Bsp119I TTCGAA 1 cut(s) 531
Bsp1286I GDGCHC 1 cut(s) 593
Bsp143I GATC 3 cut(s) 327, 1224, 1282
Bsp1720I GCTNAGC 1 cut(s) 317
BspANI GGCC 5 cut(s) 73, 247, 419, 424, 1098
BspCNI CTCAG 4 cut(s) 309, 445, 729, 1039
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 5 cut(s) 145, 273, 302, 303, 381
BspT104I TTCGAA 1 cut(s) 531
BsrDI GCAATG 1 cut(s) 393
BsrI ACTGG 2 cut(s) 273, 831
BssMI GATC 3 cut(s) 327, 1224, 1282
BssNI GRCGYC 1 cut(s) 204
Bst2UI CCWGG 1 cut(s) 1100
Bst4CI ACNGT 5 cut(s) 447, 521, 940, 1021, 1111
Bst6I CTCTTC 1 cut(s) 244
BstACI GRCGYC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 531
BstC8I GCNNGC 3 cut(s) 323, 462, 1278
BstDEI CTNAG 6 cut(s) 317, 426, 432, 689, 737, 1047
BstEII GGTNACC 1 cut(s) 950
BstF5I GGATG 7 cut(s) 251, 565, 702, 807, 819, 902, 1083
BstFNI CGCG 1 cut(s) 284
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 3 cut(s) 330, 1227, 1285
BstMAI GTCTC 2 cut(s) 195, 536
BstMBI GATC 3 cut(s) 327, 1224, 1282
BstMWI GCNNNNNNNGC 4 cut(s) 161, 292, 322, 718
BstNI CCWGG 1 cut(s) 1100
BstPI GGTNACC 1 cut(s) 950
BstSCI CCNGG 4 cut(s) 414, 419, 954, 1098
BstSLI GKGCMC 1 cut(s) 593
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 2 cut(s) 301, 1114
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 1 cut(s) 1224
BstYI RGATCY 1 cut(s) 1224
BsuRI GGCC 5 cut(s) 73, 247, 419, 424, 1098
BtsCI GGATG 7 cut(s) 251, 565, 702, 807, 819, 902, 1083
Cac8I GCNNGC 3 cut(s) 323, 462, 1278
CfoI GCGC 1 cut(s) 286
Cfr13I GGNCC 7 cut(s) 246, 271, 301, 348, 369, 417, 422
CseI GACGC 1 cut(s) 212
CviAII CATG 2 cut(s) 182, 345
DdeI CTNAG 6 cut(s) 317, 426, 432, 689, 737, 1047
DpnI GATC 3 cut(s) 329, 1226, 1284
DpnII GATC 3 cut(s) 327, 1224, 1282
DraI TTTAAA 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 1096
Eam1104I CTCTTC 1 cut(s) 244
EarI CTCTTC 1 cut(s) 244
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 4 cut(s) 271, 301, 348, 369
Eco57I CTGAAG 1 cut(s) 975
Eco91I GGTNACC 1 cut(s) 950
EcoO109I RGGNCCY 1 cut(s) 301
EcoO65I GGTNACC 1 cut(s) 950
EcoRII CCWGG 1 cut(s) 1098
EcoRV GATATC 1 cut(s) 610
EcoT22I ATGCAT 1 cut(s) 879
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 2 cut(s) 185, 348
FalI AAGNNNNNCTT 2 cut(s) 223, 255
FaqI GGGAC 4 cut(s) 80, 86, 314, 837
FatI CATG 2 cut(s) 181, 344
FauI CCCGC 1 cut(s) 157
FauNDI CATATG 1 cut(s) 706
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 8 cut(s) 153, 156, 179, 287, 290, 293, 314, 1128
FokI GGATG 7 cut(s) 238, 572, 709, 814, 826, 909, 1090
Fsp4HI GCNGC 8 cut(s) 153, 156, 179, 287, 290, 293, 314, 1128
FspBI CTAG 1 cut(s) 1026
GlaI GCGC 1 cut(s) 285
GluI GCNGC 8 cut(s) 153, 156, 179, 287, 290, 293, 314, 1128
HaeIII GGCC 5 cut(s) 73, 247, 419, 424, 1098
HapII CCGG 5 cut(s) 80, 383, 415, 420, 955
HgaI GACGC 1 cut(s) 212
HhaI GCGC 1 cut(s) 286
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 2 cut(s) 185, 348
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 9 cut(s) 185, 234, 469, 544, 770, 808, 905, 1250, 1291
HpaII CCGG 5 cut(s) 80, 383, 415, 420, 955
HphI GGTGA 5 cut(s) 418, 511, 539, 883, 962
Hpy166II GTNNAC 5 cut(s) 202, 409, 443, 591, 1011
Hpy188I TCNGA 5 cut(s) 435, 813, 1117, 1165, 1207
Hpy8I GTNNAC 5 cut(s) 202, 409, 443, 591, 1011
Hpy99I CGWCG 2 cut(s) 92, 206
HpyAV CCTTC 4 cut(s) 742, 760, 787, 811
HpyCH4III ACNGT 5 cut(s) 447, 521, 940, 1021, 1111
HpyCH4V TGCA 6 cut(s) 576, 591, 721, 877, 973, 1222
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 292, 322, 718
HpyF3I CTNAG 6 cut(s) 317, 426, 432, 689, 737, 1047
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 2 cut(s) 185, 348
HspAI GCGC 1 cut(s) 284
KflI GGGWCCC 1 cut(s) 301
Kzo9I GATC 3 cut(s) 327, 1224, 1282
LmnI GCTCC 3 cut(s) 54, 149, 385
Lsp1109I GCAGC 2 cut(s) 301, 1114
LweI GCATC 3 cut(s) 141, 550, 864
MaeI CTAG 1 cut(s) 1026
MaeIII GTNAC 4 cut(s) 499, 580, 931, 950
MalI GATC 3 cut(s) 329, 1226, 1284
MboI GATC 3 cut(s) 327, 1224, 1282
MboII GAAGA 9 cut(s) 54, 130, 162, 165, 231, 252, 464, 775, 802
MflI RGATCY 1 cut(s) 1224
MhlI GDGCHC 1 cut(s) 593
MlsI TGGCCA 1 cut(s) 1098
MluCI AATT 3 cut(s) 223, 526, 1235
MluNI TGGCCA 1 cut(s) 1098
MlyI GAGTC 6 cut(s) 179, 228, 538, 779, 1244, 1300
MmeI TCCRAC 3 cut(s) 96, 882, 1254
Mox20I TGGCCA 1 cut(s) 1098
Mph1103I ATGCAT 1 cut(s) 879
MscI TGGCCA 1 cut(s) 1098
MseI TTAA 4 cut(s) 59, 107, 123, 516
Msp20I TGGCCA 1 cut(s) 1098
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 5 cut(s) 80, 383, 415, 420, 955
MspR9I CCNGG 4 cut(s) 416, 421, 956, 1100
MvaI CCWGG 1 cut(s) 1100
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 4 cut(s) 161, 292, 322, 718
NciI CCSGG 3 cut(s) 416, 421, 956
NdeI CATATG 1 cut(s) 706
NdeII GATC 3 cut(s) 327, 1224, 1282
NlaIII CATG 2 cut(s) 185, 348
NlaIV GGNNCC 5 cut(s) 145, 273, 302, 303, 381
NmuCI GTSAC 3 cut(s) 499, 580, 950
NsiI ATGCAT 1 cut(s) 879
NspV TTCGAA 1 cut(s) 531
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 3 cut(s) 469, 808, 905
PflFI GACNNNGTC 1 cut(s) 92
PflMI CCANNNNNTGG 1 cut(s) 69
PkrI GCNGC 8 cut(s) 154, 157, 180, 288, 291, 294, 315, 1129
PleI GAGTC 6 cut(s) 179, 228, 538, 778, 1244, 1299
PpsI GAGTC 6 cut(s) 179, 228, 538, 778, 1244, 1299
PpuMI RGGWCCY 1 cut(s) 301
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 1098
PspEI GGTNACC 1 cut(s) 950
PspGI CCWGG 1 cut(s) 1098
PspN4I GGNNCC 5 cut(s) 145, 273, 302, 303, 381
PspPI GGNCC 7 cut(s) 246, 271, 301, 348, 369, 417, 422
PspPPI RGGWCCY 1 cut(s) 301
PsuI RGATCY 1 cut(s) 1224
PsyI GACNNNGTC 1 cut(s) 92
SalI GTCGAC 1 cut(s) 200
SaqAI TTAA 4 cut(s) 59, 107, 123, 516
SatI GCNGC 8 cut(s) 153, 156, 179, 287, 290, 293, 314, 1128
Sau3AI GATC 3 cut(s) 327, 1224, 1282
Sau96I GGNCC 7 cut(s) 246, 271, 301, 348, 369, 417, 422
SchI GAGTC 6 cut(s) 179, 228, 538, 779, 1244, 1300
ScrFI CCNGG 4 cut(s) 416, 421, 956, 1100
SduI GDGCHC 1 cut(s) 593
SfaNI GCATC 3 cut(s) 141, 550, 864
SfuI TTCGAA 1 cut(s) 531
SinI GGWCC 4 cut(s) 271, 301, 348, 369
SmlI CTYRAG 1 cut(s) 1184
SmoI CTYRAG 1 cut(s) 1184
Sse9I AATT 3 cut(s) 223, 526, 1235
SspMI CTAG 1 cut(s) 1026
StyD4I CCNGG 4 cut(s) 414, 419, 954, 1098
TaaI ACNGT 5 cut(s) 447, 521, 940, 1021, 1111
TaqI TCGA 4 cut(s) 201, 242, 330, 531
TasI AATT 3 cut(s) 223, 526, 1235
TauI GCSGC 6 cut(s) 155, 158, 181, 289, 295, 316
TfiI GAWTC 3 cut(s) 469, 808, 905
Tru1I TTAA 4 cut(s) 59, 107, 123, 516
Tru9I TTAA 4 cut(s) 59, 107, 123, 516
TseFI GTSAC 3 cut(s) 499, 580, 950
TseI GCWGC 2 cut(s) 289, 1127
Tsp45I GTSAC 3 cut(s) 499, 580, 950
TspDTI ATGAA 1 cut(s) 830
Tth111I GACNNNGTC 1 cut(s) 92
Van91I CCANNNNNTGG 1 cut(s) 69
VneI GTGCAC 1 cut(s) 589
VpaK11BI GGWCC 4 cut(s) 271, 301, 348, 369
XapI RAATTY 1 cut(s) 1235
XmiI GTMKAC 1 cut(s) 201
XspI CTAG 1 cut(s) 1026
Zsp2I ATGCAT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.