AT5G46630

adaptor complexes medium subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012274
Physical Location & Seq
Forward (+)
18920334 .. 18923551
3218 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46630.2

Sequence Viewer

Length: 1317 bp
ATGCCGGTGGCTGCTTCCGCCATCTATTTTCTGAATCTCCGTGGCGATGTACTCATAAATCGCACCTACCGAGATGACGTCGGGGGAAACATGGTGGATGCATTTCGTACGCATATAATGCAGACGAAAGAGTTAGGGAATTGTCCTGTGCGTCAGATTGGTGGCTGCTCCTTTGTTTATATGCGAATCAGCAATGTCTACATCGTCATTGTTGTCAGTAGCAATGCTAATGTTGCTTGTGGATTCAAATTTGTTGTTGAGGCTGTAGCTTTGTTCAAATCATACTTTGGTGGAGCTTTTGATGAAGACGCTATTAGAAATAACTTTGTTCTGATTTATGAATTGTTGGATGAGATTATGGACTTTGGCTATCCACAAAATCTCTCACCTGAAATTTTAAAACTTTATATCACTCAGGAAGGTGTACGGTCACCATTTTCATCGAAGCCAAAAGACAAACCTGTTCCAAATGCAACATTACAAGTTACAGGTGCTGTTGGTTGGAGAAGAGAGGGCCTTGCTTACAAAAAGAATGAGGTGTTTCTGGATATTGTGGAAAGTGTAAACCTTCTGATGTCCTCTAAAGGCAATGTTCTTCGGTGTGATGTGACGGGGAAGGTTTTAATGAAATGTTTCCTTTCGGGAATGCCTGATTTGAAGTTGGGGTTGAATGATAAAATTGGTCTTGAGAAGGAATCAGAAATGAAATCTCGCCCAGCTAAGAGTGGTAAAACCATTGAGCTTGATGATGTCACATTTCACCAGTGTGTGAACCTGACCAGATTCAACTCCGAGAAGACTGTTAGTTTTGTACCACCGGATGGAGAGTTTGAACTGATGAAGTATCGAATAACAGAGGGTGTAAATCTGCCTTTCCGTGTATTACCAACAATCAAAGAACTTGGTCGTACACGTATGGAAGTAAATGTCAAGGTCAAAAGTGTGTTTGGTGCTAAAATGTTTGCCCTTGGGGTCGTGGTTAAAATTCCAGTGCCAAAACAAACAGCCAAGACCAATTTCCAAGTGACAACTGGTCGCGCTAAATACAATCCATCAATCGATTGCTTGGTTTGGAAGATAAGAAAATTCCCAGGGCAAACAGAGTCCACGCTAAGTGCAGAAATTGAGTTGATCTCAACAATGGGAGAGAAGAAATCTTGGACAAGGCCACCGATCCAAATGGAATTTCAGGTGCCAATGTTCACAGCATCTGGTCTGCGAGTTCGTTTTCTCAAGGTTTGGGAGAAGAGCGGTTACAACACGGTCGAGTGGGTTCGATACATAACGAAAGCGGGATCTTACGAGATCAGATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adap_comp_sub

Protein Analysis

438

Amino Acids

49.32

Weight (kDa)

9.27

Isoelectric Point (pI)

31.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 167 - 413 5e-85 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012274)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46630 AT5G46630
fragaria_vesca FvH4_5g21870 FvH4_5g21870
malus_domestica MD06G1032900.v1.1 MD16G1276300.v1.1
prunus_persica Prupe.5G038900_v2.0.a1
pyrus_communis pycom06g02720
rosa_chinensis RchiOBHm_Chr7g0209421
rosa_laevigata RLG00000003139
rosa_multiflora Rmu_ssc0000038.1_g000017
rosa_roxburghii Rroxscaffold_3G00249490
rosa_rugosa Rorug07G0112300 Rorug07G0112400
rosa_samantha Rh7AG246300 Rh7BG241300 Rh7CG261900 Rh7DG252600
rosa_wichuraiana Rw7G020840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 1192
AccB7I CCANNNNNTGG 1 cut(s) 821
AccBSI CCGCTC 1 cut(s) 1251
AccI GTMKAC 1 cut(s) 198
AccII CGCG 1 cut(s) 1038
AciI CCGC 3 cut(s) 18, 1251, 1292
AclWI GGATC 2 cut(s) 1168, 1303
AcsI RAATTY 5 cut(s) 248, 393, 984, 1085, 1184
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 5 cut(s) 51, 109, 426, 813, 910
AfiI CCNNNNNNNGG 1 cut(s) 821
AflIII ACRYGT 1 cut(s) 911
AgsI TTSAA 6 cut(s) 247, 277, 658, 670, 787, 833
AhdI GACNNNNNGTC 1 cut(s) 1032
AjnI CCWGG 1 cut(s) 1090
AjuI GAANNNNNNNTTGG 4 cut(s) 1001, 1033, 1170, 1202
AleI CACNNNNGTG 1 cut(s) 765
AluBI AGCT 4 cut(s) 269, 296, 719, 742
AluI AGCT 4 cut(s) 269, 296, 719, 742
AlwI GGATC 2 cut(s) 1168, 1303
AlwNI CAGNNNCTG 3 cut(s) 494, 1211, 1314
AoxI GGCC 2 cut(s) 514, 1166
ApeKI GCWGC 2 cut(s) 11, 165
ApoI RAATTY 5 cut(s) 248, 393, 984, 1085, 1184
Asp700I GAANNNNTTC 1 cut(s) 632
AspLEI GCGC 1 cut(s) 1040
AspS9I GGNCC 1 cut(s) 514
AsuHPI GGTGA 3 cut(s) 378, 423, 752
BanI GGYRCC 1 cut(s) 1192
BarI GAAGNNNNNNTAC 2 cut(s) 1238, 1270
BbsI GAAGAC 2 cut(s) 312, 803
BbvI GCAGC 1 cut(s) 152
BccI CCATC 3 cut(s) 29, 815, 1060
BciT130I CCWGG 1 cut(s) 1092
BfmI CTRYAG 1 cut(s) 264
BisI GCNGC 2 cut(s) 12, 166
BlsI GCNGC 2 cut(s) 13, 167
Bme1390I CCNGG 1 cut(s) 1092
BmeRI GACNNNNNGTC 1 cut(s) 1032
BmgT120I GGNCC 1 cut(s) 514
BmiI GGNNCC 1 cut(s) 1194
BmrFI CCNGG 1 cut(s) 1092
BmsI GCATC 3 cut(s) 88, 1217, 1301
BpiI GAAGAC 2 cut(s) 312, 803
BplI GAGNNNNNCTC 2 cut(s) 1118, 1150
BpuEI CTTGAG 2 cut(s) 707, 1217
Bsa29I ATCGAT 1 cut(s) 1059
BsaAI YACGTR 1 cut(s) 914
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 4 cut(s) 40, 967, 1090, 1091
BsaWI WCCGGW 1 cut(s) 817
Bsc4I CCNNNNNNNGG 1 cut(s) 821
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 3 cut(s) 763, 989, 1036
Bse3DI GCAATG 3 cut(s) 199, 229, 595
BseBI CCWGG 1 cut(s) 1092
BseCI ATCGAT 1 cut(s) 1059
BseDI CCNNGG 4 cut(s) 40, 967, 1090, 1091
BseGI GGATG 3 cut(s) 103, 355, 826
BseLI CCNNNNNNNGG 1 cut(s) 821
BseMI GCAATG 3 cut(s) 199, 229, 595
BseMII CTCAG 1 cut(s) 428
BseNI ACTGG 3 cut(s) 763, 989, 1036
BseXI GCAGC 1 cut(s) 152
BseYI CCCAGC 1 cut(s) 715
BsgI GTGCAG 1 cut(s) 1137
Bsh1236I CGCG 1 cut(s) 1038
Bsh1285I CGRYCG 1 cut(s) 1266
BshFI GGCC 2 cut(s) 516, 1168
BshNI GGYRCC 1 cut(s) 1192
BshVI ATCGAT 1 cut(s) 1059
BsiEI CGRYCG 1 cut(s) 1266
BsiSI CCGG 2 cut(s) 5, 818
BsiWI CGTACG 1 cut(s) 107
BslI CCNNNNNNNGG 1 cut(s) 821
BsmI GAATGC 1 cut(s) 651
BsnI GGCC 2 cut(s) 516, 1168
Bsp143I GATC 4 cut(s) 1131, 1173, 1295, 1305
BspACI CCGC 3 cut(s) 18, 1251, 1292
BspANI GGCC 2 cut(s) 516, 1168
BspCNI CTCAG 1 cut(s) 427
BspDI ATCGAT 1 cut(s) 1059
BspFNI CGCG 1 cut(s) 1038
BspLI GGNNCC 1 cut(s) 1194
BspPI GGATC 2 cut(s) 1168, 1303
BspQI GCTCTTC 1 cut(s) 1241
BspT107I GGYRCC 1 cut(s) 1192
BsrBI CCGCTC 1 cut(s) 1251
BsrDI GCAATG 3 cut(s) 199, 229, 595
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 3 cut(s) 763, 989, 1036
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 4 cut(s) 40, 967, 1090, 1091
BssMI GATC 4 cut(s) 1131, 1173, 1295, 1305
BssNI GRCGYC 1 cut(s) 78
BssT1I CCWWGG 1 cut(s) 967
Bst2UI CCWGG 1 cut(s) 1092
Bst4CI ACNGT 3 cut(s) 429, 802, 1264
Bst6I CTCTTC 2 cut(s) 502, 1241
BstACI GRCGYC 1 cut(s) 78
BstAPI GCANNNNNTGC 1 cut(s) 118
BstBAI YACGTR 1 cut(s) 914
BstDEI CTNAG 3 cut(s) 414, 720, 1112
BstDSI CCRYGG 1 cut(s) 40
BstEII GGTNACC 1 cut(s) 429
BstF5I GGATG 3 cut(s) 103, 355, 826
BstFNI CGCG 1 cut(s) 1038
BstHHI GCGC 1 cut(s) 1040
BstKTI GATC 4 cut(s) 1134, 1176, 1298, 1308
BstMBI GATC 4 cut(s) 1131, 1173, 1295, 1305
BstMCI CGRYCG 1 cut(s) 1266
BstMWI GCNNNNNNNGC 3 cut(s) 17, 118, 233
BstNI CCWGG 1 cut(s) 1092
BstPI GGTNACC 1 cut(s) 429
BstSCI CCNGG 1 cut(s) 1090
BstSFI CTRYAG 1 cut(s) 264
BstUI CGCG 1 cut(s) 1038
BstV1I GCAGC 1 cut(s) 152
BstV2I GAAGAC 2 cut(s) 312, 803
BstX2I RGATCY 1 cut(s) 1295
BstYI RGATCY 1 cut(s) 1295
Bsu15I ATCGAT 1 cut(s) 1059
BsuRI GGCC 2 cut(s) 516, 1168
BsuTUI ATCGAT 1 cut(s) 1059
BtgI CCRYGG 1 cut(s) 40
BtgZI GCGATG 1 cut(s) 60
BtsCI GGATG 3 cut(s) 103, 355, 826
BtsIMutI CAGTG 2 cut(s) 770, 996
CaiI CAGNNNCTG 3 cut(s) 494, 1211, 1314
CfoI GCGC 1 cut(s) 1040
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 1 cut(s) 514
ClaI ATCGAT 1 cut(s) 1059
CseI GACGC 2 cut(s) 140, 317
Csp6I GTAC 5 cut(s) 50, 108, 425, 812, 909
CviAII CATG 1 cut(s) 91
CviQI GTAC 5 cut(s) 50, 108, 425, 812, 909
DdeI CTNAG 3 cut(s) 414, 720, 1112
DpnI GATC 4 cut(s) 1133, 1175, 1297, 1307
DpnII GATC 4 cut(s) 1131, 1173, 1295, 1305
DraI TTTAAA 1 cut(s) 399
DriI GACNNNNNGTC 1 cut(s) 1032
Eam1104I CTCTTC 2 cut(s) 502, 1241
Eam1105I GACNNNNNGTC 1 cut(s) 1032
EarI CTCTTC 2 cut(s) 502, 1241
EciI GGCGGA 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 967
Eco91I GGTNACC 1 cut(s) 429
EcoO109I RGGNCCY 1 cut(s) 514
EcoO65I GGTNACC 1 cut(s) 429
EcoRII CCWGG 1 cut(s) 1090
EcoT14I CCWWGG 1 cut(s) 967
EcoT22I ATGCAT 1 cut(s) 103
ErhI CCWWGG 1 cut(s) 967
FaeI CATG 1 cut(s) 94
FatI CATG 1 cut(s) 90
FauI CCCGC 1 cut(s) 1285
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 2 cut(s) 12, 166
FokI GGATG 3 cut(s) 110, 362, 833
Fsp4HI GCNGC 2 cut(s) 12, 166
GlaI GCGC 1 cut(s) 1039
GluI GCNGC 2 cut(s) 12, 166
GsaI CCCAGC 1 cut(s) 719
HaeIII GGCC 2 cut(s) 516, 1168
HapII CCGG 2 cut(s) 5, 818
HgaI GACGC 2 cut(s) 140, 317
HhaI GCGC 1 cut(s) 1040
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 1 cut(s) 94
Hin6I GCGC 1 cut(s) 1038
HinP1I GCGC 1 cut(s) 1038
HinfI GANTC 6 cut(s) 34, 186, 243, 695, 783, 1103
HpaII CCGG 2 cut(s) 5, 818
HphI GGTGA 3 cut(s) 378, 423, 752
Hpy166II GTNNAC 7 cut(s) 199, 425, 565, 772, 911, 1107, 1203
Hpy188I TCNGA 7 cut(s) 33, 156, 333, 573, 700, 793, 1310
Hpy188III TCNNGA 4 cut(s) 416, 545, 642, 686
Hpy8I GTNNAC 7 cut(s) 199, 425, 565, 772, 911, 1107, 1203
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 4 cut(s) 413, 578, 610, 685
HpyCH4III ACNGT 3 cut(s) 429, 802, 1264
HpyCH4IV ACGT 2 cut(s) 78, 913
HpyCH4V TGCA 4 cut(s) 101, 121, 473, 1118
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 118, 233
HpyF3I CTNAG 3 cut(s) 414, 720, 1112
HpySE526I ACGT 2 cut(s) 78, 913
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 1 cut(s) 94
HspAI GCGC 1 cut(s) 1038
Kzo9I GATC 4 cut(s) 1131, 1173, 1295, 1305
LguI GCTCTTC 1 cut(s) 1241
LmnI GCTCC 2 cut(s) 173, 293
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 3 cut(s) 88, 1217, 1301
MaeII ACGT 2 cut(s) 78, 913
MaeIII GTNAC 6 cut(s) 429, 484, 607, 751, 1024, 1253
MalI GATC 4 cut(s) 1133, 1175, 1297, 1307
MbiI CCGCTC 1 cut(s) 1251
MboI GATC 4 cut(s) 1131, 1173, 1295, 1305
MboII GAAGA 7 cut(s) 317, 519, 587, 808, 1087, 1162, 1258
MflI RGATCY 1 cut(s) 1295
MlyI GAGTC 1 cut(s) 1112
MmeI TCCRAC 2 cut(s) 327, 482
MnlI CCTC 5 cut(s) 253, 505, 529, 589, 850
Mph1103I ATGCAT 1 cut(s) 103
MroXI GAANNNNTTC 1 cut(s) 632
MseI TTAA 3 cut(s) 398, 623, 981
MslI CAYNNNNRTG 1 cut(s) 765
MspI CCGG 2 cut(s) 5, 818
MspR9I CCNGG 1 cut(s) 1092
Mva1269I GAATGC 1 cut(s) 651
MvaI CCWGG 1 cut(s) 1092
MvnI CGCG 1 cut(s) 1038
MwoI GCNNNNNNNGC 3 cut(s) 17, 118, 233
NdeII GATC 4 cut(s) 1131, 1173, 1295, 1305
NlaIII CATG 1 cut(s) 94
NlaIV GGNNCC 1 cut(s) 1194
NmuCI GTSAC 4 cut(s) 429, 607, 751, 1024
NsiI ATGCAT 1 cut(s) 103
OliI CACNNNNGTG 1 cut(s) 765
PasI CCCWGGG 1 cut(s) 1091
PciSI GCTCTTC 1 cut(s) 1241
PcsI WCGNNNNNNNCGW 1 cut(s) 67
PctI GAATGC 1 cut(s) 651
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 5 cut(s) 34, 186, 243, 695, 783
Pfl23II CGTACG 1 cut(s) 107
PflMI CCANNNNNTGG 1 cut(s) 821
PkrI GCNGC 2 cut(s) 13, 167
PleI GAGTC 1 cut(s) 1111
PpsI GAGTC 1 cut(s) 1111
Ppu21I YACGTR 1 cut(s) 914
Psp6I CCWGG 1 cut(s) 1090
PspEI GGTNACC 1 cut(s) 429
PspFI CCCAGC 1 cut(s) 715
PspGI CCWGG 1 cut(s) 1090
PspLI CGTACG 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 1194
PspPI GGNCC 1 cut(s) 514
PstNI CAGNNNCTG 3 cut(s) 494, 1211, 1314
PsuI RGATCY 1 cut(s) 1295
RsaI GTAC 5 cut(s) 51, 109, 426, 813, 910
RsaNI GTAC 5 cut(s) 50, 108, 425, 812, 909
RseI CAYNNNNRTG 1 cut(s) 765
SapI GCTCTTC 1 cut(s) 1241
SaqAI TTAA 3 cut(s) 398, 623, 981
SatI GCNGC 2 cut(s) 12, 166
Sau3AI GATC 4 cut(s) 1131, 1173, 1295, 1305
Sau96I GGNCC 1 cut(s) 514
SchI GAGTC 1 cut(s) 1112
ScrFI CCNGG 1 cut(s) 1092
SfaNI GCATC 3 cut(s) 88, 1217, 1301
SfcI CTRYAG 1 cut(s) 264
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 2 cut(s) 686, 1232
SmoI CTYRAG 2 cut(s) 686, 1232
SsiI CCGC 3 cut(s) 18, 1251, 1292
StyD4I CCNGG 1 cut(s) 1090
StyI CCWWGG 1 cut(s) 967
TaaI ACNGT 3 cut(s) 429, 802, 1264
TaiI ACGT 2 cut(s) 81, 916
TaqI TCGA 5 cut(s) 443, 847, 1059, 1266, 1276
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 5 cut(s) 34, 186, 243, 695, 783
Tru1I TTAA 3 cut(s) 398, 623, 981
Tru9I TTAA 3 cut(s) 398, 623, 981
TscAI CASTG 2 cut(s) 770, 996
TseFI GTSAC 4 cut(s) 429, 607, 751, 1024
TseI GCWGC 2 cut(s) 11, 165
Tsp45I GTSAC 4 cut(s) 429, 607, 751, 1024
TspDTI ATGAA 6 cut(s) 318, 354, 429, 641, 719, 854
TspGWI ACGGA 2 cut(s) 29, 866
TspRI CASTG 2 cut(s) 770, 996
Van91I CCANNNNNTGG 1 cut(s) 821
XapI RAATTY 5 cut(s) 248, 393, 984, 1085, 1184
XcmI CCANNNNNNNNNTGG 1 cut(s) 1028
XmiI GTMKAC 1 cut(s) 198
XmnI GAANNNNTTC 1 cut(s) 632
ZraI GACGTC 1 cut(s) 79
Zsp2I ATGCAT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.