MD06G1032900.v1.1

Belongs to the adaptor complexes medium subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012274
Physical Location & Seq
Reverse (-)
3937559 .. 3952675
15117 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1032900.v1.1.491

Sequence Viewer

Length: 1317 bp
ATGCCAGTGGCTGCTTCCGCCATCTACTTCTTGAACCTCCGCGGTGATGTTCTCATCAACCGTCTCTATCGTGACGATGTTGGCGGAAATATGGTGGATGCTTTCCGCACGCATATTATGCAAACAAAAGAACTTGGAACATGTCCTGTGCGGCAGATTGGTGGATGCTCTTTCTTTTACATGAGAATCAGCAACGTTTACATTGTGATTGTAGTCAGCAGCAATGCCAATGTAGCCTGTGCATTCAAGTTTGTTGTCGAGGCTGTTGCGCTGTTCAAATCTTATTTTGGTGGGGCTTTTGACGAAGATGCAATTCGTAATAATTTTGTTCTGATTTATGAGCTATTGGATGAAATTATGGATTTTGGTTACCCACAGAATCTTTCTCCAGAAATTTTGAAGCTTTACATCACTCAGGAAGGAGTGCGCTCTCCATTCTCTTCCAAGCCTACAGATAAACCTGTCCCTAATGCAACACTACAAGTTACAGGTGCTGTTGGTTGGCGTAGAGAGGGCCTTGTTTATAAAAAGAATGAGGTCTTCTTGGATATTGTGGAAAGTGTAAATCTTCTTATGTCTTCCAAAGGTAGTGTTCTGCGCTGTGATGTAACCGGGAAGGTTCTAATGAAGTGCTTCCTCTCTGGAATGCCCGATTTGAAGTTGGGCTTGAATGATAAAATTGGCCTCGAGAAAGAGTCGCAACTTAAAGCCCGTCCCACCAAAAGTGGGAAAACTATCGAGCTTGATGATGTTACTTTCCATCAATGTGTCAACTTGACGAGATTTAACTCAGAGAAGACAGTTAGTTTTGTGCCACCTGATGGTGAATTTGAATTGATGAAGTATCGTATTACTGAGGGTGTGAATCTTCCATTCCGAGTATTGCCTACAATTAAAGAACTTGGCAGAACTCGCATTGAAGTAAATGTTAAGGTCAAGAGCGTTTTTGGTGCAAAAATGTTTGCTCTTGGGGTTGTCATCAAAATTCCTGTGCCAAAACAAACAGCAAAGACAAGTTTTCAAGTGACATCTGGTCGAGCAAAATATAATGCTGCTATTGATTGCTTGGTCTGGAAGATAAGGAAATTTCCAGGACAAACGGAGCCAACCTTGAGTGCAGAAGTTGAGCTGATTTCCACGATGACAGAAAAGAAATCTTGGACAAGACCACCAATTCAGATGGAGTTCCAGGTTCCGATGTTTACAGCGTCTGGTTTACGTGTGCGTTTTCTCAAGGTATGGGAAAAGAGTGGATACAACACTGTTGAGTGGGTTCGCTATATCACGAAAGCTGGTTCATACGAGATTAGGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adap_comp_sub

Protein Analysis

439

Amino Acids

49.3

Weight (kDa)

9.28

Isoelectric Point (pI)

31.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 167 - 438 2.3e-94 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012274)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46630 AT5G46630
fragaria_vesca FvH4_5g21870 FvH4_5g21870
malus_domestica MD06G1032900.v1.1 MD16G1276300.v1.1
prunus_persica Prupe.5G038900_v2.0.a1
pyrus_communis pycom06g02720
rosa_chinensis RchiOBHm_Chr7g0209421
rosa_laevigata RLG00000003139
rosa_multiflora Rmu_ssc0000038.1_g000017
rosa_roxburghii Rroxscaffold_3G00249490
rosa_rugosa Rorug07G0112300 Rorug07G0112400
rosa_samantha Rh7AG246300 Rh7BG241300 Rh7CG261900 Rh7DG252600
rosa_wichuraiana Rw7G020840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AccB7I CCANNNNNTGG 1 cut(s) 821
AccII CGCG 1 cut(s) 42
AciI CCGC 6 cut(s) 18, 40, 42, 84, 106, 151
AclI AACGTT 1 cut(s) 195
AcsI RAATTY 4 cut(s) 393, 827, 984, 1085
AfiI CCNNNNNNNGG 2 cut(s) 726, 821
AflIII ACRYGT 2 cut(s) 140, 1219
AgsI TTSAA 9 cut(s) 34, 247, 277, 400, 658, 670, 833, 920, 1022
AhdI GACNNNNNGTC 1 cut(s) 1032
AjnI CCWGG 2 cut(s) 1090, 1188
AluBI AGCT 5 cut(s) 343, 403, 742, 1129, 1292
AluI AGCT 5 cut(s) 343, 403, 742, 1129, 1292
Alw26I GTCTC 1 cut(s) 68
AlwNI CAGNNNCTG 3 cut(s) 11, 494, 1211
Ama87I CYCGRG 1 cut(s) 686
AoxI GGCC 2 cut(s) 514, 682
ApeKI GCWGC 3 cut(s) 11, 219, 1052
ApoI RAATTY 4 cut(s) 393, 827, 984, 1085
Asp700I GAANNNNTTC 1 cut(s) 632
AspLEI GCGC 3 cut(s) 271, 429, 600
AspS9I GGNCC 1 cut(s) 514
AsuC2I CCSGG 1 cut(s) 613
AsuHPI GGTGA 2 cut(s) 56, 836
AvaI CYCGRG 1 cut(s) 686
BbsI GAAGAC 3 cut(s) 532, 570, 803
BbvI GCAGC 2 cut(s) 231, 1039
BccI CCATC 4 cut(s) 29, 768, 815, 1174
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 2 cut(s) 1092, 1190
BciVI GTATCC 1 cut(s) 1247
BcnI CCSGG 1 cut(s) 613
BcoDI GTCTC 1 cut(s) 68
BfaI CTAG 1 cut(s) 1315
BfmI CTRYAG 1 cut(s) 450
BfuI GTATCC 1 cut(s) 1247
BisI GCNGC 4 cut(s) 12, 152, 220, 1053
BlsI GCNGC 4 cut(s) 13, 153, 221, 1054
Bme1390I CCNGG 3 cut(s) 613, 1092, 1190
BmeRI GACNNNNNGTC 1 cut(s) 1032
BmeT110I CYCGRG 1 cut(s) 686
BmgT120I GGNCC 1 cut(s) 514
BmiI GGNNCC 2 cut(s) 1104, 1194
BmrFI CCNGG 3 cut(s) 613, 1092, 1190
BmsI GCATC 3 cut(s) 88, 155, 298
BpiI GAAGAC 3 cut(s) 532, 570, 803
BpmI CTGGAG 1 cut(s) 372
BpuEI CTTGAG 2 cut(s) 1132, 1217
BpuMI CCSGG 1 cut(s) 613
BsaAI YACGTR 1 cut(s) 1220
BsaJI CCNNGG 1 cut(s) 40
Bsc4I CCNNNNNNNGG 2 cut(s) 726, 821
Bse1I ACTGG 1 cut(s) 5
Bse3DI GCAATG 1 cut(s) 229
BseBI CCWGG 2 cut(s) 1092, 1190
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 3 cut(s) 103, 170, 355
BseLI CCNNNNNNNGG 2 cut(s) 726, 821
BseMI GCAATG 1 cut(s) 229
BseMII CTCAG 3 cut(s) 428, 804, 846
BseNI ACTGG 1 cut(s) 5
BseXI GCAGC 2 cut(s) 231, 1039
BsgI GTGCAG 1 cut(s) 1137
Bsh1236I CGCG 1 cut(s) 42
BshFI GGCC 2 cut(s) 516, 684
BsiHKCI CYCGRG 1 cut(s) 686
BsiSI CCGG 1 cut(s) 612
BslFI GGGAC 2 cut(s) 449, 699
BslI CCNNNNNNNGG 2 cut(s) 726, 821
BsmAI GTCTC 1 cut(s) 68
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 2 cut(s) 449, 699
BsmI GAATGC 2 cut(s) 242, 651
BsnI GGCC 2 cut(s) 516, 684
BsoBI CYCGRG 1 cut(s) 686
BspACI CCGC 6 cut(s) 18, 40, 42, 84, 106, 151
BspANI GGCC 2 cut(s) 516, 684
BspCNI CTCAG 3 cut(s) 427, 803, 847
BspFNI CGCG 1 cut(s) 42
BspLI GGNNCC 2 cut(s) 1104, 1194
BsrDI GCAATG 1 cut(s) 229
BsrI ACTGG 1 cut(s) 5
BssECI CCNNGG 1 cut(s) 40
Bst2UI CCWGG 2 cut(s) 1092, 1190
Bst4CI ACNGT 3 cut(s) 62, 802, 1264
Bst6I CTCTTC 1 cut(s) 445
BstAPI GCANNNNNTGC 1 cut(s) 118
BstBAI YACGTR 1 cut(s) 1220
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 3 cut(s) 414, 790, 855
BstDSI CCRYGG 1 cut(s) 40
BstEII GGTNACC 1 cut(s) 368
BstF5I GGATG 3 cut(s) 103, 170, 355
BstFNI CGCG 1 cut(s) 42
BstHHI GCGC 3 cut(s) 271, 429, 600
BstMAI GTCTC 1 cut(s) 68
BstMWI GCNNNNNNNGC 4 cut(s) 17, 118, 233, 912
BstNI CCWGG 2 cut(s) 1092, 1190
BstNSI RCATGY 1 cut(s) 144
BstPI GGTNACC 1 cut(s) 368
BstSCI CCNGG 3 cut(s) 611, 1090, 1188
BstSFI CTRYAG 1 cut(s) 450
BstUI CGCG 1 cut(s) 42
BstV1I GCAGC 2 cut(s) 231, 1039
BstV2I GAAGAC 3 cut(s) 532, 570, 803
BsuI GTATCC 1 cut(s) 1247
BsuRI GGCC 2 cut(s) 516, 684
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 3 cut(s) 103, 170, 355
BtsIMutI CAGTG 2 cut(s) 12, 1260
Cac8I GCNNGC 1 cut(s) 110
CaiI CAGNNNCTG 3 cut(s) 11, 494, 1211
CfoI GCGC 3 cut(s) 271, 429, 600
Cfr13I GGNCC 1 cut(s) 514
Cfr42I CCGCGG 1 cut(s) 43
CseI GACGC 1 cut(s) 1197
CviAII CATG 2 cut(s) 141, 181
DdeI CTNAG 3 cut(s) 414, 790, 855
DriI GACNNNNNGTC 1 cut(s) 1032
Eam1104I CTCTTC 1 cut(s) 445
Eam1105I GACNNNNNGTC 1 cut(s) 1032
EarI CTCTTC 1 cut(s) 445
EciI GGCGGA 2 cut(s) 7, 99
Eco88I CYCGRG 1 cut(s) 686
Eco91I GGTNACC 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 514
EcoO65I GGTNACC 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 1090, 1188
Esp3I CGTCTC 1 cut(s) 68
FaeI CATG 2 cut(s) 144, 184
FalI AAGNNNNNCTT 2 cut(s) 650, 682
FaqI GGGAC 2 cut(s) 449, 699
FatI CATG 2 cut(s) 140, 180
Fnu4HI GCNGC 4 cut(s) 12, 152, 220, 1053
FokI GGATG 3 cut(s) 110, 177, 362
Fsp4HI GCNGC 4 cut(s) 12, 152, 220, 1053
FspBI CTAG 1 cut(s) 1315
GlaI GCGC 3 cut(s) 270, 428, 599
GluI GCNGC 4 cut(s) 12, 152, 220, 1053
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 2 cut(s) 516, 684
HapII CCGG 1 cut(s) 612
HgaI GACGC 1 cut(s) 1197
HhaI GCGC 3 cut(s) 271, 429, 600
Hin1II CATG 2 cut(s) 144, 184
Hin6I GCGC 3 cut(s) 269, 427, 598
HinP1I GCGC 3 cut(s) 269, 427, 598
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 4 cut(s) 186, 379, 695, 865
HpaII CCGG 1 cut(s) 612
HphI GGTGA 2 cut(s) 56, 836
Hpy166II GTNNAC 4 cut(s) 199, 772, 1203, 1217
Hpy188I TCNGA 5 cut(s) 333, 793, 878, 1179, 1197
Hpy188III TCNNGA 9 cut(s) 31, 71, 389, 416, 642, 688, 937, 1072, 1285
Hpy8I GTNNAC 4 cut(s) 199, 772, 1203, 1217
HpyAV CCTTC 2 cut(s) 413, 610
HpyCH4III ACNGT 3 cut(s) 62, 802, 1264
HpyCH4IV ACGT 2 cut(s) 195, 1219
HpyCH4V TGCA 6 cut(s) 121, 242, 311, 473, 953, 1118
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 118, 233, 912
HpyF3I CTNAG 3 cut(s) 414, 790, 855
HpySE526I ACGT 2 cut(s) 195, 1219
Hsp92II CATG 2 cut(s) 144, 184
HspAI GCGC 3 cut(s) 269, 427, 598
KspI CCGCGG 1 cut(s) 43
LmnI GCTCC 1 cut(s) 1102
Lsp1109I GCAGC 2 cut(s) 231, 1039
LweI GCATC 3 cut(s) 88, 155, 298
MaeI CTAG 1 cut(s) 1315
MaeII ACGT 2 cut(s) 195, 1219
MaeIII GTNAC 6 cut(s) 71, 368, 484, 607, 751, 1024
MboII GAAGA 8 cut(s) 317, 432, 532, 560, 570, 808, 860, 1087
MlyI GAGTC 1 cut(s) 704
MnlI CCTC 7 cut(s) 47, 253, 505, 529, 647, 695, 850
MroXI GAANNNNTTC 1 cut(s) 632
MseI TTAA 4 cut(s) 705, 786, 894, 930
MslI CAYNNNNRTG 1 cut(s) 765
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 1 cut(s) 612
MspR9I CCNGG 3 cut(s) 613, 1092, 1190
Mva1269I GAATGC 2 cut(s) 242, 651
MvaI CCWGG 2 cut(s) 1092, 1190
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 4 cut(s) 17, 118, 233, 912
NciI CCSGG 1 cut(s) 613
NlaIII CATG 2 cut(s) 144, 184
NlaIV GGNNCC 2 cut(s) 1104, 1194
NmuCI GTSAC 2 cut(s) 71, 1024
NspI RCATGY 1 cut(s) 144
PaeR7I CTCGAG 1 cut(s) 686
PciI ACATGT 1 cut(s) 140
PctI GAATGC 2 cut(s) 242, 651
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 3 cut(s) 186, 379, 865
PflMI CCANNNNNTGG 1 cut(s) 821
PfoI TCCNGGA 1 cut(s) 1090
PkrI GCNGC 4 cut(s) 13, 153, 221, 1054
PleI GAGTC 1 cut(s) 703
PpsI GAGTC 1 cut(s) 703
Ppu21I YACGTR 1 cut(s) 1220
PscI ACATGT 1 cut(s) 140
PsiI TTATAA 1 cut(s) 525
Psp1406I AACGTT 1 cut(s) 195
Psp6I CCWGG 2 cut(s) 1090, 1188
PspEI GGTNACC 1 cut(s) 368
PspGI CCWGG 2 cut(s) 1090, 1188
PspN4I GGNNCC 2 cut(s) 1104, 1194
PspPI GGNCC 1 cut(s) 514
PstNI CAGNNNCTG 3 cut(s) 11, 494, 1211
RseI CAYNNNNRTG 1 cut(s) 765
SacII CCGCGG 1 cut(s) 43
SaqAI TTAA 4 cut(s) 705, 786, 894, 930
SatI GCNGC 4 cut(s) 12, 152, 220, 1053
Sau96I GGNCC 1 cut(s) 514
SchI GAGTC 1 cut(s) 704
ScrFI CCNGG 3 cut(s) 613, 1092, 1190
SfaNI GCATC 3 cut(s) 88, 155, 298
SfcI CTRYAG 1 cut(s) 450
Sfr274I CTCGAG 1 cut(s) 686
Sfr303I CCGCGG 1 cut(s) 43
SgrBI CCGCGG 1 cut(s) 43
SlaI CTCGAG 1 cut(s) 686
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 3 cut(s) 686, 1111, 1232
SmoI CTYRAG 3 cut(s) 686, 1111, 1232
SsiI CCGC 6 cut(s) 18, 40, 42, 84, 106, 151
SspMI CTAG 1 cut(s) 1315
StyD4I CCNGG 3 cut(s) 611, 1090, 1188
TaaI ACNGT 3 cut(s) 62, 802, 1264
TaiI ACGT 2 cut(s) 198, 1222
TaqI TCGA 4 cut(s) 258, 687, 738, 1036
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 3 cut(s) 186, 379, 865
Tru1I TTAA 4 cut(s) 705, 786, 894, 930
Tru9I TTAA 4 cut(s) 705, 786, 894, 930
TscAI CASTG 2 cut(s) 12, 1267
TseFI GTSAC 2 cut(s) 71, 1024
TseI GCWGC 3 cut(s) 11, 219, 1052
Tsp45I GTSAC 2 cut(s) 71, 1024
TspDTI ATGAA 4 cut(s) 366, 641, 854, 1287
TspGWI ACGGA 1 cut(s) 1115
TspRI CASTG 2 cut(s) 12, 1267
Van91I CCANNNNNTGG 1 cut(s) 821
XapI RAATTY 4 cut(s) 393, 827, 984, 1085
XceI RCATGY 1 cut(s) 144
XhoI CTCGAG 1 cut(s) 686
XmnI GAANNNNTTC 1 cut(s) 632
XspI CTAG 1 cut(s) 1315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.