Rorug07G0112300

Belongs to the adaptor complexes medium subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0012274
Physical Location & Seq
Forward (+)
8772548 .. 8776349
3802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0112300.1

Sequence Viewer

Length: 1317 bp
ATGGCTTCGCAGGATAAAGGTCGACCCTTACCTAAATTTGGTGAGTGGGATGTAAATAACCCAGCATCAGCTGAAGGATTTACTGTCATATTCAACAAGGCCCGAGATGATAAGAAGACCAATGCGTCTCATCCTAATGTTGTGACCCCACGAAAAGGCGATAGCAGCGTACGAAGAGGTACTAAATACAACAATGACTACCACGTCGACAATTATCCTCAATATCCCCAAAAGGTAAAGCACCAACGCACACCAACTGCATCCTTCTCCTTTCCTGTGCACGTCAGTCTGCTTGCAATCCCCGAAACCCTTATCGGAGTTGTTTTGAGAACGGTACACCAATCGAAGTGCAAGCTGTTCAGAAGAGAATCTATTCCGAATTTCAACAAGATGTCAAACATGAACAAACTCGATTTTGTTCCATTGGATTATACAGGCAAAAGGTACTTAATTTGGGTCACTGATGTCGAGATCCACCTTATTGCCCGTGATTTATTGCATACAATCCAAGAGCTTTGTCATATAGAAACAGCTCCAGACCCTCAAACTGAGAAAGATAATTCCAAGGCACTTGCCTTCATGAAACGTCACATGGATAGTGACCTACAGTTTGAGTTCATAAATGAGGATAGCGCCATAAAGCTTTGGCATGCGCTTCGCGAACGATATGGCAATGTTCGTGACTCCATACTTCCGAATACAGAAGCAGAATGGAAAGATCTTCGCTTCTCTGAATTTGACACTGTCATGCAATTTTATTCGGAAGCTCTCAGCATCAGAGCAATGATGCGTCTTTGTGGAAAAAAAATCACAGAAGACCAGTTGATTGAGAAAACCCTCAATACCTTCCCCGTATGTGCTATGAGGTCCTCTGACTTATTCCGGACTCATGTAAATGCAAGACGGATCACAAGTTTTCAACAGCTTATTGAAGCTATGGCCACTACTGAAAGACATGGCAATGAACTTGTGAAAAGAAGATTTGATAGGCTTCAAGATAGCTATCAAGAGCATCGTCCTATGGCTAGAGAAAGTCGCCATAGACGTCATGATCCATACGATCGAAATGCTCAAGAAGGTAATCGCTCAAGGCGACAATCCCACGACCGTAGGAGGCGTGATTTTGAGGGACATAGGGTTGGAAACCATAGAGCCAACGTTGGCCATAGGCACCACTTTCCAACGCCACCAGTCCTAGGGACTATGCATATTGCGCCAATGCGCCTCAATCAAGGGAGAATCCTCTTTACGGTGTCTATTTCTGATATGGAACGTCTGATCAATGGACCTGAATTTGCAATGTACCTACGAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adap_comp_sub

Protein Analysis

438

Amino Acids

51.33

Weight (kDa)

9.7

Isoelectric Point (pI)

60.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 6 - 39 2.6e-18 Cleavage site for pathogenic type III effector avirulence factor Avr
Retrotran_gag_2 PF14223 157 - 284 7.3e-08 gag-polypeptide of LTR copia-type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012274)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46630 AT5G46630
fragaria_vesca FvH4_5g21870 FvH4_5g21870
malus_domestica MD06G1032900.v1.1 MD16G1276300.v1.1
prunus_persica Prupe.5G038900_v2.0.a1
pyrus_communis pycom06g02720
rosa_chinensis RchiOBHm_Chr7g0209421
rosa_laevigata RLG00000003139
rosa_multiflora Rmu_ssc0000038.1_g000017
rosa_roxburghii Rroxscaffold_3G00249490
rosa_rugosa Rorug07G0112300 Rorug07G0112400
rosa_samantha Rh7AG246300 Rh7BG241300 Rh7CG261900 Rh7DG252600
rosa_wichuraiana Rw7G020840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 124, 203
AatII GACGTC 1 cut(s) 1048
AccB1I GGYRCC 1 cut(s) 1170
AccI GTMKAC 2 cut(s) 22, 207
AccII CGCG 1 cut(s) 660
AccIII TCCGGA 1 cut(s) 882
AclI AACGTT 1 cut(s) 1158
AclWI GGATC 3 cut(s) 466, 914, 1046
AcoI YGGCCR 2 cut(s) 939, 1162
AcsI RAATTY 4 cut(s) 35, 379, 734, 1292
AcuI CTGAAG 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 1045
AfaI GTAC 5 cut(s) 171, 181, 336, 446, 1304
AfiI CCNNNNNNNGG 4 cut(s) 38, 155, 1196, 1249
AgsI TTSAA 5 cut(s) 94, 385, 920, 932, 995
AjiI CACGTC 2 cut(s) 205, 283
AluBI AGCT 9 cut(s) 71, 355, 514, 533, 643, 767, 925, 935, 1002
AluI AGCT 9 cut(s) 71, 355, 514, 533, 643, 767, 925, 935, 1002
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 1 cut(s) 132
Alw44I GTGCAC 1 cut(s) 278
AlwI GGATC 3 cut(s) 466, 914, 1046
Ama87I CYCGRG 1 cut(s) 102
Aor13HI TCCGGA 1 cut(s) 882
AoxI GGCC 3 cut(s) 99, 939, 1162
ApaLI GTGCAC 1 cut(s) 278
ApeKI GCWGC 1 cut(s) 165
ApoI RAATTY 4 cut(s) 35, 379, 734, 1292
ArsI GACNNNNNNTTYG 2 cut(s) 593, 625
Asp700I GAANNNNTTC 1 cut(s) 372
AspA2I CCTAGG 1 cut(s) 1195
AspLEI GCGC 4 cut(s) 635, 655, 1216, 1224
AspS9I GGNCC 3 cut(s) 100, 867, 1286
AsuHPI GGTGA 1 cut(s) 53
AvaI CYCGRG 1 cut(s) 102
AvaII GGWCC 2 cut(s) 867, 1286
AvrII CCTAGG 1 cut(s) 1195
BaeGI GKGCMC 1 cut(s) 282
BalI TGGCCA 2 cut(s) 941, 1164
BanI GGYRCC 1 cut(s) 1170
BbsI GAAGAC 2 cut(s) 122, 822
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 177
BcgI CGANNNNNNTGC 4 cut(s) 638, 672, 1049, 1083
BclI TGATCA 1 cut(s) 1278
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 2 cut(s) 1026, 1196
BfmI CTRYAG 1 cut(s) 605
BfoI RGCGCY 1 cut(s) 636
BglII AGATCT 1 cut(s) 718
BisI GCNGC 1 cut(s) 166
BlnI CCTAGG 1 cut(s) 1195
BlsI GCNGC 1 cut(s) 167
Bme18I GGWCC 2 cut(s) 867, 1286
BmeT110I CYCGRG 1 cut(s) 102
BmgBI CACGTC 2 cut(s) 205, 283
BmgT120I GGNCC 3 cut(s) 100, 867, 1286
BmiI GGNNCC 1 cut(s) 1172
BmsI GCATC 5 cut(s) 74, 269, 777, 783, 1021
BpiI GAAGAC 2 cut(s) 122, 822
BpmI CTGGAG 1 cut(s) 519
BpuEI CTTGAG 2 cut(s) 1056, 1072
BsaBI GATNNNNATC 1 cut(s) 1002
BsaHI GRCGYC 1 cut(s) 1045
BsaJI CCNNGG 2 cut(s) 564, 1195
BsaWI WCCGGW 1 cut(s) 882
Bsc4I CCNNNNNNNGG 4 cut(s) 38, 155, 1196, 1249
Bse1I ACTGG 2 cut(s) 820, 1190
Bse3DI GCAATG 4 cut(s) 679, 789, 967, 1305
Bse8I GATNNNNATC 1 cut(s) 1002
BseAI TCCGGA 1 cut(s) 882
BseDI CCNNGG 2 cut(s) 564, 1195
BseGI GGATG 3 cut(s) 55, 130, 260
BseJI GATNNNNATC 1 cut(s) 1002
BseLI CCNNNNNNNGG 4 cut(s) 38, 155, 1196, 1249
BseMI GCAATG 4 cut(s) 679, 789, 967, 1305
BseMII CTCAG 2 cut(s) 540, 784
BseNI ACTGG 2 cut(s) 820, 1190
BseSI GKGCMC 1 cut(s) 282
BseXI GCAGC 1 cut(s) 177
BseYI CCCAGC 1 cut(s) 61
Bsh1236I CGCG 1 cut(s) 660
Bsh1285I CGRYCG 2 cut(s) 1063, 1108
BshFI GGCC 3 cut(s) 101, 941, 1164
BshNI GGYRCC 1 cut(s) 1170
BsiEI CGRYCG 2 cut(s) 1063, 1108
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 1 cut(s) 102
BsiSI CCGG 1 cut(s) 883
BsiWI CGTACG 1 cut(s) 169
BslFI GGGAC 2 cut(s) 1143, 1213
BslI CCNNNNNNNGG 4 cut(s) 38, 155, 1196, 1249
BsmAI GTCTC 1 cut(s) 132
BsmBI CGTCTC 1 cut(s) 132
BsmFI GGGAC 2 cut(s) 1143, 1213
BsnI GGCC 3 cut(s) 101, 941, 1164
BsoBI CYCGRG 1 cut(s) 102
Bsp1286I GDGCHC 1 cut(s) 282
Bsp13I TCCGGA 1 cut(s) 882
Bsp143I GATC 6 cut(s) 471, 718, 906, 1051, 1060, 1278
Bsp68I TCGCGA 1 cut(s) 660
BspANI GGCC 3 cut(s) 101, 941, 1164
BspCNI CTCAG 2 cut(s) 541, 783
BspEI TCCGGA 1 cut(s) 882
BspFNI CGCG 1 cut(s) 660
BspHI TCATGA 2 cut(s) 579, 1048
BspLI GGNNCC 1 cut(s) 1172
BspPI GGATC 3 cut(s) 466, 914, 1046
BspT107I GGYRCC 1 cut(s) 1170
BsrDI GCAATG 4 cut(s) 679, 789, 967, 1305
BsrI ACTGG 2 cut(s) 820, 1190
BssECI CCNNGG 2 cut(s) 564, 1195
BssMI GATC 6 cut(s) 471, 718, 906, 1051, 1060, 1278
BssNI GRCGYC 1 cut(s) 1045
BssT1I CCWWGG 2 cut(s) 564, 1195
Bst4CI ACNGT 6 cut(s) 85, 334, 609, 745, 1109, 1252
Bst6I CTCTTC 2 cut(s) 169, 358
BstACI GRCGYC 1 cut(s) 1045
BstC8I GCNNGC 3 cut(s) 294, 353, 651
BstDEI CTNAG 2 cut(s) 549, 770
BstF5I GGATG 3 cut(s) 55, 130, 260
BstFNI CGCG 1 cut(s) 660
BstH2I RGCGCY 1 cut(s) 636
BstHHI GCGC 4 cut(s) 635, 655, 1216, 1224
BstKTI GATC 6 cut(s) 474, 721, 909, 1054, 1063, 1281
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 6 cut(s) 471, 718, 906, 1051, 1060, 1278
BstMCI CGRYCG 2 cut(s) 1063, 1108
BstMWI GCNNNNNNNGC 2 cut(s) 165, 1213
BstNSI RCATGY 1 cut(s) 653
BstSFI CTRYAG 1 cut(s) 605
BstSLI GKGCMC 1 cut(s) 282
BstUI CGCG 1 cut(s) 660
BstV1I GCAGC 1 cut(s) 177
BstV2I GAAGAC 2 cut(s) 122, 822
BstX2I RGATCY 2 cut(s) 471, 718
BstYI RGATCY 2 cut(s) 471, 718
BsuRI GGCC 3 cut(s) 101, 941, 1164
BtrI CACGTC 2 cut(s) 205, 283
BtsCI GGATG 3 cut(s) 55, 130, 260
BtsIMutI CAGTG 2 cut(s) 459, 741
BtuMI TCGCGA 1 cut(s) 660
Cac8I GCNNGC 3 cut(s) 294, 353, 651
CciI TCATGA 2 cut(s) 579, 1048
CfoI GCGC 4 cut(s) 635, 655, 1216, 1224
Cfr13I GGNCC 3 cut(s) 100, 867, 1286
CseI GACGC 2 cut(s) 114, 779
Csp6I GTAC 5 cut(s) 170, 180, 335, 445, 1303
CviAII CATG 8 cut(s) 400, 580, 592, 650, 748, 890, 956, 1049
CviQI GTAC 5 cut(s) 170, 180, 335, 445, 1303
DdeI CTNAG 2 cut(s) 549, 770
DpnI GATC 6 cut(s) 473, 720, 908, 1053, 1062, 1280
DpnII GATC 6 cut(s) 471, 718, 906, 1051, 1060, 1278
DrdI GACNNNNNNGTC 2 cut(s) 124, 203
DseDI GACNNNNNNGTC 2 cut(s) 124, 203
EaeI YGGCCR 2 cut(s) 939, 1162
Eam1104I CTCTTC 2 cut(s) 169, 358
EarI CTCTTC 2 cut(s) 169, 358
Eco130I CCWWGG 2 cut(s) 564, 1195
Eco47I GGWCC 2 cut(s) 867, 1286
Eco57I CTGAAG 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 102
EcoO109I RGGNCCY 1 cut(s) 867
EcoT14I CCWWGG 2 cut(s) 564, 1195
EcoT22I ATGCAT 1 cut(s) 1209
ErhI CCWWGG 2 cut(s) 564, 1195
Esp3I CGTCTC 1 cut(s) 132
FaeI CATG 8 cut(s) 403, 583, 595, 653, 751, 893, 959, 1052
FaqI GGGAC 2 cut(s) 1143, 1213
FatI CATG 8 cut(s) 399, 579, 591, 649, 747, 889, 955, 1048
FbaI TGATCA 1 cut(s) 1278
FblI GTMKAC 2 cut(s) 22, 207
Fnu4HI GCNGC 1 cut(s) 166
FokI GGATG 3 cut(s) 62, 117, 247
Fsp4HI GCNGC 1 cut(s) 166
FspBI CTAG 2 cut(s) 1026, 1196
GlaI GCGC 4 cut(s) 634, 654, 1215, 1223
GluI GCNGC 1 cut(s) 166
GsaI CCCAGC 1 cut(s) 65
GsuI CTGGAG 1 cut(s) 519
HaeII RGCGCY 1 cut(s) 636
HaeIII GGCC 3 cut(s) 101, 941, 1164
HapII CCGG 1 cut(s) 883
HgaI GACGC 2 cut(s) 114, 779
HhaI GCGC 4 cut(s) 635, 655, 1216, 1224
Hin1I GRCGYC 1 cut(s) 1045
Hin1II CATG 8 cut(s) 403, 583, 595, 653, 751, 893, 959, 1052
Hin6I GCGC 4 cut(s) 633, 653, 1214, 1222
HinP1I GCGC 4 cut(s) 633, 653, 1214, 1222
HincII GTYRAC 2 cut(s) 23, 208
HindII GTYRAC 2 cut(s) 23, 208
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 4 cut(s) 368, 683, 886, 1239
HpaII CCGG 1 cut(s) 883
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 4 cut(s) 23, 208, 280, 337
Hpy8I GTNNAC 4 cut(s) 23, 208, 280, 337
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 6 cut(s) 68, 274, 586, 856, 1070, 1305
HpyCH4III ACNGT 6 cut(s) 85, 334, 609, 745, 1109, 1252
HpyCH4IV ACGT 6 cut(s) 204, 282, 586, 1045, 1158, 1273
HpyCH4V TGCA 9 cut(s) 260, 280, 296, 351, 499, 751, 899, 1207, 1298
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 1213
HpyF3I CTNAG 2 cut(s) 549, 770
HpySE526I ACGT 6 cut(s) 204, 282, 586, 1045, 1158, 1273
Hsp92I GRCGYC 1 cut(s) 1045
Hsp92II CATG 8 cut(s) 403, 583, 595, 653, 751, 893, 959, 1052
HspAI GCGC 4 cut(s) 633, 653, 1214, 1222
Kpn2I TCCGGA 1 cut(s) 882
Ksp22I TGATCA 1 cut(s) 1278
Kzo9I GATC 6 cut(s) 471, 718, 906, 1051, 1060, 1278
LmnI GCTCC 1 cut(s) 538
LpnPI CCDG 8 cut(s) 75, 288, 420, 549, 833, 896, 1203, 1302
Lsp1109I GCAGC 1 cut(s) 177
LweI GCATC 5 cut(s) 74, 269, 777, 783, 1021
MaeI CTAG 2 cut(s) 1026, 1196
MaeII ACGT 6 cut(s) 204, 282, 586, 1045, 1158, 1273
MaeIII GTNAC 5 cut(s) 142, 457, 587, 599, 680
MalI GATC 6 cut(s) 473, 720, 908, 1053, 1062, 1280
MboI GATC 6 cut(s) 471, 718, 906, 1051, 1060, 1278
MboII GAAGA 6 cut(s) 127, 186, 375, 713, 827, 990
MflI RGATCY 2 cut(s) 471, 718
MhlI GDGCHC 1 cut(s) 282
MlsI TGGCCA 2 cut(s) 941, 1164
MluCI AATT 8 cut(s) 35, 211, 379, 450, 559, 734, 752, 1292
MluNI TGGCCA 2 cut(s) 941, 1164
MlyI GAGTC 2 cut(s) 677, 880
MmeI TCCRAC 2 cut(s) 1120, 1205
Mox20I TGGCCA 2 cut(s) 941, 1164
Mph1103I ATGCAT 1 cut(s) 1209
MroI TCCGGA 1 cut(s) 882
MroXI GAANNNNTTC 1 cut(s) 372
MscI TGGCCA 2 cut(s) 941, 1164
MseI TTAA 1 cut(s) 449
MslI CAYNNNNRTG 4 cut(s) 135, 746, 894, 960
Msp20I TGGCCA 2 cut(s) 941, 1164
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 1 cut(s) 883
MvnI CGCG 1 cut(s) 660
MwoI GCNNNNNNNGC 2 cut(s) 165, 1213
NdeII GATC 6 cut(s) 471, 718, 906, 1051, 1060, 1278
NlaIII CATG 8 cut(s) 403, 583, 595, 653, 751, 893, 959, 1052
NlaIV GGNNCC 1 cut(s) 1172
NmuCI GTSAC 5 cut(s) 142, 457, 587, 599, 680
NruI TCGCGA 1 cut(s) 660
NsiI ATGCAT 1 cut(s) 1209
NspI RCATGY 1 cut(s) 653
PaeI GCATGC 1 cut(s) 653
PagI TCATGA 2 cut(s) 579, 1048
PcsI WCGNNNNNNNCGW 2 cut(s) 1042, 1090
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 2 cut(s) 368, 1239
Pfl23II CGTACG 1 cut(s) 169
PflFI GACNNNGTC 1 cut(s) 743
PkrI GCNGC 1 cut(s) 167
Ple19I CGATCG 1 cut(s) 1063
PleI GAGTC 2 cut(s) 677, 880
PpsI GAGTC 2 cut(s) 677, 880
PpuMI RGGWCCY 1 cut(s) 867
Psp1406I AACGTT 1 cut(s) 1158
Psp5II RGGWCCY 1 cut(s) 867
PspFI CCCAGC 1 cut(s) 61
PspLI CGTACG 1 cut(s) 169
PspN4I GGNNCC 1 cut(s) 1172
PspPI GGNCC 3 cut(s) 100, 867, 1286
PspPPI RGGWCCY 1 cut(s) 867
PsuI RGATCY 2 cut(s) 471, 718
PsyI GACNNNGTC 1 cut(s) 743
PvuI CGATCG 1 cut(s) 1063
PvuII CAGCTG 1 cut(s) 71
RruI TCGCGA 1 cut(s) 660
RsaI GTAC 5 cut(s) 171, 181, 336, 446, 1304
RsaNI GTAC 5 cut(s) 170, 180, 335, 445, 1303
RseI CAYNNNNRTG 4 cut(s) 135, 746, 894, 960
SalI GTCGAC 2 cut(s) 21, 206
SaqAI TTAA 1 cut(s) 449
SatI GCNGC 1 cut(s) 166
Sau3AI GATC 6 cut(s) 471, 718, 906, 1051, 1060, 1278
Sau96I GGNCC 3 cut(s) 100, 867, 1286
SchI GAGTC 2 cut(s) 677, 880
SduI GDGCHC 1 cut(s) 282
SfaNI GCATC 5 cut(s) 74, 269, 777, 783, 1021
SfcI CTRYAG 1 cut(s) 605
SinI GGWCC 2 cut(s) 867, 1286
SmiMI CAYNNNNRTG 4 cut(s) 135, 746, 894, 960
SmlI CTYRAG 2 cut(s) 1071, 1087
SmoI CTYRAG 2 cut(s) 1071, 1087
SphI GCATGC 1 cut(s) 653
Sse9I AATT 8 cut(s) 35, 211, 379, 450, 559, 734, 752, 1292
SspMI CTAG 2 cut(s) 1026, 1196
StyI CCWWGG 2 cut(s) 564, 1195
TaaI ACNGT 6 cut(s) 85, 334, 609, 745, 1109, 1252
TaiI ACGT 6 cut(s) 207, 285, 589, 1048, 1161, 1276
TaqI TCGA 6 cut(s) 22, 207, 344, 411, 468, 1063
TasI AATT 8 cut(s) 35, 211, 379, 450, 559, 734, 752, 1292
TfiI GAWTC 2 cut(s) 368, 1239
Tru1I TTAA 1 cut(s) 449
Tru9I TTAA 1 cut(s) 449
TscAI CASTG 2 cut(s) 466, 748
TseFI GTSAC 5 cut(s) 142, 457, 587, 599, 680
TseI GCWGC 1 cut(s) 165
Tsp45I GTSAC 5 cut(s) 142, 457, 587, 599, 680
TspDTI ATGAA 5 cut(s) 416, 568, 596, 607, 978
TspGWI ACGGA 1 cut(s) 919
TspRI CASTG 2 cut(s) 466, 748
Tth111I GACNNNGTC 1 cut(s) 743
VneI GTGCAC 1 cut(s) 278
VpaK11BI GGWCC 2 cut(s) 867, 1286
XapI RAATTY 4 cut(s) 35, 379, 734, 1292
XceI RCATGY 1 cut(s) 653
XmaJI CCTAGG 1 cut(s) 1195
XmiI GTMKAC 2 cut(s) 22, 207
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 2 cut(s) 1026, 1196
ZraI GACGTC 1 cut(s) 1046
Zsp2I ATGCAT 1 cut(s) 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.