MD16G1276300.v1.1

Belongs to the adaptor complexes medium subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012274
Physical Location & Seq
Forward (+)
37265355 .. 37276873
11519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1276300.v1.1.491

Sequence Viewer

Length: 1317 bp
ATGCCGGTGGCTGCTTCCGCCGTTTATTTCTTGAACCTCCGCGGTGATGTTCTCATCAACCGTCTCTATCGTGACGATGTTGGAGGAAATATGGTGGATGCTTTCCGCATGCATATTATGCAAACAAAAGAACTTGGAACATGTCCTGTGCGGCAGATTGGTGGATGCTCTTTCTGTTACATGAGAATCAGCAATGTTTACATTGTAATTGTAGTCAGCAGCAATGCCAATATAGCCTGTGCATTCAAGTTTGTTGTTGAGGCTGTTGCACTGTTCAAATCTTATTTTGGTGGGGCTTTTGATGAAGATGCAATTCGTAATAATTTTGTTCTGATTTATGAGCTATTAGACGAAATTATGGATTTTGGTTACCCACAGAATCTTTCTCCAGAAATTTTAAAGCTTTACATCACTCAGGAAGGAGTGCGCTCTCCATTCTCTTCTAAGCCTGCAGATAAACCTGTCCCTAATGCAACACTACAAGTTACAGGTGCTGTTGGTTGGCGCAGAGAGGGCCTTGCTTATAAAAAGAATGAGGTCTTCTTGGATATTGTGGAAAGTGTAAATCTTCTTATGTCTTCCAAAGGTAGTGTTCTACGTTGTGATGTAACCGGGAAGATTCTAATGAAATGCTTCCTCTCTGGGATGCCTGATTTGAAATTGGGATTGAATGATAAAATTGGCCTTGAGAAAGAATCACAGCTTAAATCCCGTCCCACAAAAAGTGGGAAAACTATCGAGCTCGACGATGTTACTTTCCATCAATGTGTAAACTTGACGAGGTTTAACTCAGAGAAGACAGTTAGTTTTGTGCCACCTGATGGTGAATTTGAATTGATGAAGTATCGTATTACTGAGGGTGTTAATCTTCCATTCCGAGTATTGCCTACAATTAAGGAACTCGGCAGAACTCGTATTGAAGTAAATGTTAAGGTCAAGAGCGTTTTCGGTGCAAAAATGTTTGCTCTTGGGGTTGTCATCAAAATTCCTGTGCCAAAACAAACAGCAAAAACAAGTTTTCAAGTGACGTCTGGTCGAGCAAAATACAATGCGGCTATTGATTGCTTGGTCTGGAAGATAAGGAAATTTCCAGGACAAACAGAGCCAACTTTGAGTGCAGAAGTTGAGCTGATTTCCACGATGACAGAAAAGAAATCTTGGACAAGGCCACCAATCCAAATGGAGTTCCAGGTTCCGATGTTTACAGCGTCTGGTTTACGTGTCCGTTTTCTGAAGGTATGGGAAAAGAGTGGATACAACACTGTTGAGTGGGTTCGCTATATCACGAAAGCTGGTTCTTACGAGATTAGGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04721 ko04961 ko05016 map04144 map04721 map04961 map05016
Pfam Domains
Protein Families
Adap_comp_sub

Protein Analysis

439

Amino Acids

49.26

Weight (kDa)

9.25

Isoelectric Point (pI)

34.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 167 - 438 8.2e-95 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012274)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46630 AT5G46630
fragaria_vesca FvH4_5g21870 FvH4_5g21870
malus_domestica MD06G1032900.v1.1 MD16G1276300.v1.1
prunus_persica Prupe.5G038900_v2.0.a1
pyrus_communis pycom06g02720
rosa_chinensis RchiOBHm_Chr7g0209421
rosa_laevigata RLG00000003139
rosa_multiflora Rmu_ssc0000038.1_g000017
rosa_roxburghii Rroxscaffold_3G00249490
rosa_rugosa Rorug07G0112300 Rorug07G0112400
rosa_samantha Rh7AG246300 Rh7BG241300 Rh7CG261900 Rh7DG252600
rosa_wichuraiana Rw7G020840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AatII GACGTC 1 cut(s) 1031
AccB7I CCANNNNNTGG 1 cut(s) 821
AccII CGCG 1 cut(s) 42
AciI CCGC 6 cut(s) 18, 40, 42, 106, 151, 1052
AcsI RAATTY 4 cut(s) 393, 827, 984, 1085
AcuI CTGAAG 1 cut(s) 1253
AcyI GRCGYC 1 cut(s) 1028
AfiI CCNNNNNNNGG 1 cut(s) 821
AflIII ACRYGT 2 cut(s) 140, 1219
AgsI TTSAA 8 cut(s) 34, 247, 277, 658, 670, 833, 920, 1022
AhdI GACNNNNNGTC 1 cut(s) 1032
AjnI CCWGG 2 cut(s) 1090, 1188
AluBI AGCT 6 cut(s) 343, 403, 703, 742, 1129, 1292
AluI AGCT 6 cut(s) 343, 403, 703, 742, 1129, 1292
Alw21I GWGCWC 1 cut(s) 744
Alw26I GTCTC 1 cut(s) 68
AlwNI CAGNNNCTG 2 cut(s) 494, 1211
AoxI GGCC 3 cut(s) 514, 682, 1166
ApeKI GCWGC 2 cut(s) 11, 219
ApoI RAATTY 4 cut(s) 393, 827, 984, 1085
Asp700I GAANNNNTTC 1 cut(s) 632
AspLEI GCGC 2 cut(s) 429, 507
AspS9I GGNCC 1 cut(s) 514
AsuC2I CCSGG 1 cut(s) 613
AsuHPI GGTGA 2 cut(s) 56, 836
BanII GRGCYC 1 cut(s) 744
BbsI GAAGAC 3 cut(s) 532, 570, 803
Bbv12I GWGCWC 1 cut(s) 744
BbvI GCAGC 1 cut(s) 231
BccI CCATC 2 cut(s) 768, 815
BceAI ACGGC 1 cut(s) 5
BciT130I CCWGG 2 cut(s) 1092, 1190
BciVI GTATCC 1 cut(s) 1247
BcnI CCSGG 1 cut(s) 613
BcoDI GTCTC 1 cut(s) 68
BfaI CTAG 1 cut(s) 1315
BfmI CTRYAG 1 cut(s) 450
BfuI GTATCC 1 cut(s) 1247
BisI GCNGC 4 cut(s) 12, 152, 220, 1053
BlsI GCNGC 4 cut(s) 13, 153, 221, 1054
Bme1390I CCNGG 3 cut(s) 613, 1092, 1190
BmeRI GACNNNNNGTC 1 cut(s) 1032
BmgT120I GGNCC 1 cut(s) 514
BmiI GGNNCC 1 cut(s) 1194
BmrFI CCNGG 3 cut(s) 613, 1092, 1190
BmsI GCATC 4 cut(s) 88, 155, 298, 636
BpiI GAAGAC 3 cut(s) 532, 570, 803
BpmI CTGGAG 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 707
BpuMI CCSGG 1 cut(s) 613
BsaAI YACGTR 1 cut(s) 1220
BsaHI GRCGYC 1 cut(s) 1028
BsaJI CCNNGG 1 cut(s) 40
Bsc4I CCNNNNNNNGG 1 cut(s) 821
Bse118I RCCGGY 1 cut(s) 4
Bse3DI GCAATG 2 cut(s) 199, 229
BseBI CCWGG 2 cut(s) 1092, 1190
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 3 cut(s) 103, 170, 651
BseLI CCNNNNNNNGG 1 cut(s) 821
BseMI GCAATG 2 cut(s) 199, 229
BseMII CTCAG 3 cut(s) 428, 804, 846
BseXI GCAGC 1 cut(s) 231
BsgI GTGCAG 1 cut(s) 1137
Bsh1236I CGCG 1 cut(s) 42
BshFI GGCC 3 cut(s) 516, 684, 1168
BsiHKAI GWGCWC 1 cut(s) 744
BsiSI CCGG 2 cut(s) 5, 612
BslFI GGGAC 2 cut(s) 449, 699
BslI CCNNNNNNNGG 1 cut(s) 821
BsmAI GTCTC 1 cut(s) 68
BsmBI CGTCTC 1 cut(s) 68
BsmFI GGGAC 2 cut(s) 449, 699
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 3 cut(s) 516, 684, 1168
Bsp1286I GDGCHC 1 cut(s) 744
BspACI CCGC 6 cut(s) 18, 40, 42, 106, 151, 1052
BspANI GGCC 3 cut(s) 516, 684, 1168
BspCNI CTCAG 3 cut(s) 427, 803, 847
BspFNI CGCG 1 cut(s) 42
BspLI GGNNCC 1 cut(s) 1194
BspMAI CTGCAG 1 cut(s) 454
BsrDI GCAATG 2 cut(s) 199, 229
BsrFI RCCGGY 1 cut(s) 4
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 40
BssNI GRCGYC 1 cut(s) 1028
Bst2UI CCWGG 2 cut(s) 1092, 1190
Bst4CI ACNGT 4 cut(s) 62, 273, 802, 1264
Bst6I CTCTTC 1 cut(s) 445
BstACI GRCGYC 1 cut(s) 1028
BstAPI GCANNNNNTGC 1 cut(s) 118
BstBAI YACGTR 1 cut(s) 1220
BstC8I GCNNGC 2 cut(s) 110, 450
BstDEI CTNAG 4 cut(s) 414, 444, 790, 855
BstDSI CCRYGG 1 cut(s) 40
BstEII GGTNACC 1 cut(s) 368
BstF5I GGATG 3 cut(s) 103, 170, 651
BstFNI CGCG 1 cut(s) 42
BstHHI GCGC 2 cut(s) 429, 507
BstMAI GTCTC 1 cut(s) 68
BstMWI GCNNNNNNNGC 4 cut(s) 17, 118, 233, 513
BstNI CCWGG 2 cut(s) 1092, 1190
BstNSI RCATGY 2 cut(s) 112, 144
BstPI GGTNACC 1 cut(s) 368
BstSCI CCNGG 3 cut(s) 611, 1090, 1188
BstSFI CTRYAG 1 cut(s) 450
BstUI CGCG 1 cut(s) 42
BstV1I GCAGC 1 cut(s) 231
BstV2I GAAGAC 3 cut(s) 532, 570, 803
BsuI GTATCC 1 cut(s) 1247
BsuRI GGCC 3 cut(s) 516, 684, 1168
BtgI CCRYGG 1 cut(s) 40
BtsCI GGATG 3 cut(s) 103, 170, 651
BtsIMutI CAGTG 2 cut(s) 269, 1260
Cac8I GCNNGC 2 cut(s) 110, 450
CaiI CAGNNNCTG 2 cut(s) 494, 1211
CfoI GCGC 2 cut(s) 429, 507
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 1 cut(s) 514
Cfr42I CCGCGG 1 cut(s) 43
CseI GACGC 1 cut(s) 1197
CviAII CATG 3 cut(s) 109, 141, 181
DdeI CTNAG 4 cut(s) 414, 444, 790, 855
DraI TTTAAA 1 cut(s) 399
DriI GACNNNNNGTC 1 cut(s) 1032
Eam1104I CTCTTC 1 cut(s) 445
Eam1105I GACNNNNNGTC 1 cut(s) 1032
EarI CTCTTC 1 cut(s) 445
EciI GGCGGA 1 cut(s) 7
Ecl136II GAGCTC 1 cut(s) 742
Eco24I GRGCYC 1 cut(s) 744
Eco53kI GAGCTC 1 cut(s) 742
Eco57I CTGAAG 1 cut(s) 1253
Eco91I GGTNACC 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 742
EcoO109I RGGNCCY 1 cut(s) 514
EcoO65I GGTNACC 1 cut(s) 368
EcoRII CCWGG 2 cut(s) 1090, 1188
EcoT22I ATGCAT 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 744
Esp3I CGTCTC 1 cut(s) 68
FaeI CATG 3 cut(s) 112, 144, 184
FaqI GGGAC 2 cut(s) 449, 699
FatI CATG 3 cut(s) 108, 140, 180
Fnu4HI GCNGC 4 cut(s) 12, 152, 220, 1053
FokI GGATG 3 cut(s) 110, 177, 658
FriOI GRGCYC 1 cut(s) 744
Fsp4HI GCNGC 4 cut(s) 12, 152, 220, 1053
FspBI CTAG 1 cut(s) 1315
GlaI GCGC 2 cut(s) 428, 506
GluI GCNGC 4 cut(s) 12, 152, 220, 1053
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 3 cut(s) 516, 684, 1168
HapII CCGG 2 cut(s) 5, 612
HgaI GACGC 1 cut(s) 1197
HhaI GCGC 2 cut(s) 429, 507
Hin1I GRCGYC 1 cut(s) 1028
Hin1II CATG 3 cut(s) 112, 144, 184
Hin6I GCGC 2 cut(s) 427, 505
HinP1I GCGC 2 cut(s) 427, 505
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 4 cut(s) 186, 379, 619, 695
HpaII CCGG 2 cut(s) 5, 612
HphI GGTGA 2 cut(s) 56, 836
Hpy166II GTNNAC 4 cut(s) 199, 772, 1203, 1217
Hpy188I TCNGA 5 cut(s) 333, 793, 878, 1197, 1233
Hpy188III TCNNGA 7 cut(s) 31, 71, 389, 416, 937, 1072, 1285
Hpy8I GTNNAC 4 cut(s) 199, 772, 1203, 1217
Hpy99I CGWCG 1 cut(s) 749
HpyAV CCTTC 2 cut(s) 413, 1228
HpyCH4III ACNGT 4 cut(s) 62, 273, 802, 1264
HpyCH4IV ACGT 3 cut(s) 598, 1028, 1219
HpyCH4V TGCA 9 cut(s) 112, 121, 242, 269, 311, 452, 473, 953, 1118
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 118, 233, 513
HpyF3I CTNAG 4 cut(s) 414, 444, 790, 855
HpySE526I ACGT 3 cut(s) 598, 1028, 1219
Hsp92I GRCGYC 1 cut(s) 1028
Hsp92II CATG 3 cut(s) 112, 144, 184
HspAI GCGC 2 cut(s) 427, 505
KspI CCGCGG 1 cut(s) 43
Lsp1109I GCAGC 1 cut(s) 231
LweI GCATC 4 cut(s) 88, 155, 298, 636
MaeI CTAG 1 cut(s) 1315
MaeII ACGT 3 cut(s) 598, 1028, 1219
MaeIII GTNAC 7 cut(s) 71, 176, 368, 484, 607, 751, 1024
MboII GAAGA 9 cut(s) 317, 432, 532, 560, 570, 628, 808, 860, 1087
MhlI GDGCHC 1 cut(s) 744
MmeI TCCRAC 1 cut(s) 61
MnlI CCTC 8 cut(s) 47, 77, 253, 505, 529, 647, 774, 850
Mph1103I ATGCAT 1 cut(s) 114
MroXI GAANNNNTTC 1 cut(s) 632
MseI TTAA 6 cut(s) 398, 705, 786, 864, 894, 930
MslI CAYNNNNRTG 1 cut(s) 765
MspA1I CMGCKG 1 cut(s) 42
MspI CCGG 2 cut(s) 5, 612
MspR9I CCNGG 3 cut(s) 613, 1092, 1190
Mva1269I GAATGC 1 cut(s) 242
MvaI CCWGG 2 cut(s) 1092, 1190
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 4 cut(s) 17, 118, 233, 513
NciI CCSGG 1 cut(s) 613
NlaIII CATG 3 cut(s) 112, 144, 184
NlaIV GGNNCC 1 cut(s) 1194
NmeAIII GCCGAG 1 cut(s) 882
NmuCI GTSAC 2 cut(s) 71, 1024
NsiI ATGCAT 1 cut(s) 114
NspI RCATGY 2 cut(s) 112, 144
PaeI GCATGC 1 cut(s) 112
PciI ACATGT 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 744
PctI GAATGC 1 cut(s) 242
PdmI GAANNNNTTC 1 cut(s) 632
PfeI GAWTC 4 cut(s) 186, 379, 619, 695
PflMI CCANNNNNTGG 1 cut(s) 821
PfoI TCCNGGA 1 cut(s) 1090
PkrI GCNGC 4 cut(s) 13, 153, 221, 1054
Ppu21I YACGTR 1 cut(s) 1220
PscI ACATGT 1 cut(s) 140
PsiI TTATAA 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 744
Psp6I CCWGG 2 cut(s) 1090, 1188
PspEI GGTNACC 1 cut(s) 368
PspGI CCWGG 2 cut(s) 1090, 1188
PspN4I GGNNCC 1 cut(s) 1194
PspPI GGNCC 1 cut(s) 514
PstI CTGCAG 1 cut(s) 454
PstNI CAGNNNCTG 2 cut(s) 494, 1211
RseI CAYNNNNRTG 1 cut(s) 765
SacI GAGCTC 1 cut(s) 744
SacII CCGCGG 1 cut(s) 43
SaqAI TTAA 6 cut(s) 398, 705, 786, 864, 894, 930
SatI GCNGC 4 cut(s) 12, 152, 220, 1053
Sau96I GGNCC 1 cut(s) 514
ScrFI CCNGG 3 cut(s) 613, 1092, 1190
SduI GDGCHC 1 cut(s) 744
SfaNI GCATC 4 cut(s) 88, 155, 298, 636
SfcI CTRYAG 1 cut(s) 450
Sfr303I CCGCGG 1 cut(s) 43
SgrBI CCGCGG 1 cut(s) 43
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 1 cut(s) 686
SmoI CTYRAG 1 cut(s) 686
SphI GCATGC 1 cut(s) 112
SsiI CCGC 6 cut(s) 18, 40, 42, 106, 151, 1052
SspMI CTAG 1 cut(s) 1315
SstI GAGCTC 1 cut(s) 744
StyD4I CCNGG 3 cut(s) 611, 1090, 1188
TaaI ACNGT 4 cut(s) 62, 273, 802, 1264
TaiI ACGT 3 cut(s) 601, 1031, 1222
TaqI TCGA 3 cut(s) 738, 744, 1036
TauI GCSGC 2 cut(s) 154, 1055
TfiI GAWTC 4 cut(s) 186, 379, 619, 695
Tru1I TTAA 6 cut(s) 398, 705, 786, 864, 894, 930
Tru9I TTAA 6 cut(s) 398, 705, 786, 864, 894, 930
TscAI CASTG 2 cut(s) 276, 1267
TseFI GTSAC 2 cut(s) 71, 1024
TseI GCWGC 2 cut(s) 11, 219
Tsp45I GTSAC 2 cut(s) 71, 1024
TspDTI ATGAA 3 cut(s) 318, 641, 854
TspGWI ACGGA 1 cut(s) 1214
TspRI CASTG 2 cut(s) 276, 1267
Van91I CCANNNNNTGG 1 cut(s) 821
XapI RAATTY 4 cut(s) 393, 827, 984, 1085
XceI RCATGY 2 cut(s) 112, 144
XmnI GAANNNNTTC 1 cut(s) 632
XspI CTAG 1 cut(s) 1315
ZraI GACGTC 1 cut(s) 1029
Zsp2I ATGCAT 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.