AT5G47000

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003752
Physical Location & Seq
Reverse (-)
19068803 .. 19070333
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G47000.1

Sequence Viewer

Length: 1005 bp
ATGTCGAATATGCAATTCTCTCGTGGTTTTAATCCCTTTGTTATCCTCTTCTGTCTCGCCGTGGTTGCACCAATTATATCGGCTGATGTCGCTATCTTGAGAACGGACTATTACCAAAAAACATGTCCTGATTTCCACAAAATTGTGCGTGAAGCCGTTACAACCAAACAAGTCCAACAACCAACAACTGCGGCCGGGACCCTCCGTCTCTTTTTCCATGATTGTTTCCTTGAAGGTTGTGATGCATCTGTCTTGATCGCGACCAACTCGTTCAACAAAGCGGAACGCGATGATGATCTCAATGATTCCCTCCCGGGAGATGCTTTTGACATCGTCACCCGCATCAAGACAGCTCTCGAGTTGTCTTGTCCTGGTGTAGTATCATGCGCGGATATTCTAGCGCAGGCTACACGTGACCTTGTCACAATGGTAGGAGGACCTTACTTTGACGTAAAGCTTGGTCGTAAAGACGGATTCGAATCCAAAGCTCATAAAGTCAGAGGAAATGTCCCAATGGCAAACCAGACTGTTCCTGACATCCACGGGATATTCAAGAAAAACGGTTTTAGTCTTCGCGAGATGGTAGCATTAAGCGGGGCTCACACCATTGGATTCTCTCACTGCAAAGAGTTTTCCGACAGACTCTACGGATCCCGTGCTGATAAAGAAATCAACCCGCGATTCGCAGCCGCTCTCAAAGATCTTTGCAAAAACCACACCGTGGATGATACAATCGCCGCGTTTAACGACGTGATGACTCCAGGAAAGTTCGACAACATGTACTTCAAGAACCTAAAGCGAGGGCTAGGGCTTTTAGCGTCTGACCACATCCTTATTAAAGACAACAGCACCAAGCCGTTTGTGGATCTATACGCAACTAACGAGACAGCATTCTTTGAGGATTTCGCTCGTGCGATGGAGAAACTTGGCACGGTCGGCGTCAAGGGCGATAAAGATGGAGAAGTGAGACGTAGGTGCGACCACTTCAACAATCTCAACGTTTAA

Protein Analysis

334

Amino Acids

37.01

Weight (kDa)

6.75

Isoelectric Point (pI)

24.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 49 - 294 2.5e-77 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 721
AccBSI CCGCTC 1 cut(s) 692
AccII CGCG 6 cut(s) 260, 288, 389, 576, 679, 740
AciI CCGC 8 cut(s) 191, 281, 340, 389, 594, 677, 690, 738
AclI AACGTT 1 cut(s) 999
AclWI GGATC 3 cut(s) 645, 658, 873
AcoI YGGCCR 1 cut(s) 192
AcvI CACGTG 1 cut(s) 413
AcyI GRCGYC 1 cut(s) 939
AdeI CACNNNGTG 1 cut(s) 721
AfaI GTAC 1 cut(s) 782
AfiI CCNNNNNNNGG 1 cut(s) 721
AflIII ACRYGT 3 cut(s) 122, 410, 777
AgsI TTSAA 5 cut(s) 233, 274, 553, 787, 988
AhdI GACNNNNNGTC 1 cut(s) 204
AjiI CACGTC 1 cut(s) 751
AjnI CCWGG 2 cut(s) 370, 760
AluBI AGCT 3 cut(s) 353, 457, 488
AluI AGCT 3 cut(s) 353, 457, 488
Alw26I GTCTC 4 cut(s) 59, 212, 878, 961
AlwI GGATC 3 cut(s) 645, 658, 873
Ama87I CYCGRG 2 cut(s) 313, 356
AoxI GGCC 1 cut(s) 192
ApeKI GCWGC 1 cut(s) 686
ArsI GACNNNNNNTTYG 2 cut(s) 109, 141
AspLEI GCGC 2 cut(s) 389, 403
AspS9I GGNCC 2 cut(s) 198, 437
AsuC2I CCSGG 3 cut(s) 196, 314, 315
AsuHPI GGTGA 1 cut(s) 328
AsuII TTCGAA 1 cut(s) 477
AvaI CYCGRG 2 cut(s) 313, 356
AvaII GGWCC 2 cut(s) 198, 437
BamHI GGATCC 1 cut(s) 650
BanII GRGCYC 1 cut(s) 601
BauI CACGAG 2 cut(s) 21, 909
BbrPI CACGTG 1 cut(s) 413
BbsI GAAGAC 1 cut(s) 563
BbvI GCAGC 1 cut(s) 698
BccI CCATC 3 cut(s) 574, 910, 950
BceAI ACGGC 3 cut(s) 44, 140, 841
BcgI CGANNNNNNTGC 1 cut(s) 36
BciT130I CCWGG 2 cut(s) 372, 762
BcnI CCSGG 3 cut(s) 196, 314, 315
BcoDI GTCTC 4 cut(s) 59, 212, 878, 961
BfaI CTAG 2 cut(s) 398, 806
BglII AGATCT 1 cut(s) 700
BisI GCNGC 4 cut(s) 192, 687, 690, 738
BlsI GCNGC 4 cut(s) 193, 688, 691, 739
Bme1390I CCNGG 5 cut(s) 196, 314, 315, 372, 762
Bme18I GGWCC 2 cut(s) 198, 437
BmeRI GACNNNNNGTC 1 cut(s) 204
BmeT110I CYCGRG 2 cut(s) 313, 356
BmgBI CACGTC 1 cut(s) 751
BmgT120I GGNCC 2 cut(s) 198, 437
BmiI GGNNCC 3 cut(s) 199, 200, 652
BmrFI CCNGG 5 cut(s) 196, 314, 315, 372, 762
BmsI GCATC 4 cut(s) 232, 254, 310, 351
BpiI GAAGAC 1 cut(s) 563
BpmI CTGGAG 1 cut(s) 744
Bpu14I TTCGAA 1 cut(s) 477
BpuEI CTTGAG 1 cut(s) 118
BpuMI CCSGG 3 cut(s) 196, 314, 315
BsaAI YACGTR 1 cut(s) 413
BsaBI GATNNNNATC 2 cut(s) 294, 478
BsaHI GRCGYC 1 cut(s) 939
BsaJI CCNNGG 4 cut(s) 60, 313, 541, 720
Bsc4I CCNNNNNNNGG 1 cut(s) 721
Bse8I GATNNNNATC 2 cut(s) 294, 478
BseBI CCWGG 2 cut(s) 372, 762
BseDI CCNNGG 4 cut(s) 60, 313, 541, 720
BseGI GGATG 3 cut(s) 537, 730, 828
BseJI GATNNNNATC 2 cut(s) 294, 478
BseLI CCNNNNNNNGG 1 cut(s) 721
BseX3I CGGCCG 1 cut(s) 192
BseXI GCAGC 1 cut(s) 698
Bsh1236I CGCG 6 cut(s) 260, 288, 389, 576, 679, 740
Bsh1285I CGRYCG 2 cut(s) 195, 936
BshFI GGCC 1 cut(s) 194
BsiEI CGRYCG 2 cut(s) 195, 936
BsiHKCI CYCGRG 2 cut(s) 313, 356
BsiSI CCGG 2 cut(s) 195, 314
BslFI GGGAC 2 cut(s) 211, 494
BslI CCNNNNNNNGG 1 cut(s) 721
BsmAI GTCTC 4 cut(s) 59, 212, 878, 961
BsmBI CGTCTC 2 cut(s) 212, 961
BsmFI GGGAC 2 cut(s) 211, 494
BsmI GAATGC 1 cut(s) 890
BsnI GGCC 1 cut(s) 194
BsoBI CYCGRG 2 cut(s) 313, 356
Bsp119I TTCGAA 1 cut(s) 477
Bsp1286I GDGCHC 1 cut(s) 601
Bsp143I GATC 5 cut(s) 255, 295, 650, 700, 865
Bsp68I TCGCGA 2 cut(s) 260, 576
BspACI CCGC 8 cut(s) 191, 281, 340, 389, 594, 677, 690, 738
BspANI GGCC 1 cut(s) 194
BspFNI CGCG 6 cut(s) 260, 288, 389, 576, 679, 740
BspLI GGNNCC 3 cut(s) 199, 200, 652
BspPI GGATC 3 cut(s) 645, 658, 873
BspT104I TTCGAA 1 cut(s) 477
BsrBI CCGCTC 1 cut(s) 692
BssECI CCNNGG 4 cut(s) 60, 313, 541, 720
BssMI GATC 5 cut(s) 255, 295, 650, 700, 865
BssNI GRCGYC 1 cut(s) 939
BssSI CACGAG 2 cut(s) 21, 909
Bst2BI CACGAG 2 cut(s) 21, 909
Bst2UI CCWGG 2 cut(s) 372, 762
Bst4CI ACNGT 4 cut(s) 529, 563, 721, 934
Bst6I CTCTTC 1 cut(s) 53
BstACI GRCGYC 1 cut(s) 939
BstBAI YACGTR 1 cut(s) 413
BstBI TTCGAA 1 cut(s) 477
BstC8I GCNNGC 1 cut(s) 405
BstDSI CCRYGG 3 cut(s) 60, 541, 720
BstF5I GGATG 3 cut(s) 537, 730, 828
BstFNI CGCG 6 cut(s) 260, 288, 389, 576, 679, 740
BstHHI GCGC 2 cut(s) 389, 403
BstKTI GATC 5 cut(s) 258, 298, 653, 703, 868
BstMAI GTCTC 4 cut(s) 59, 212, 878, 961
BstMBI GATC 5 cut(s) 255, 295, 650, 700, 865
BstMCI CGRYCG 2 cut(s) 195, 936
BstMWI GCNNNNNNNGC 4 cut(s) 65, 89, 936, 945
BstNI CCWGG 2 cut(s) 372, 762
BstNSI RCATGY 2 cut(s) 126, 781
BstSCI CCNGG 5 cut(s) 194, 312, 313, 370, 760
BstUI CGCG 6 cut(s) 260, 288, 389, 576, 679, 740
BstV1I GCAGC 1 cut(s) 698
BstV2I GAAGAC 1 cut(s) 563
BstX2I RGATCY 3 cut(s) 650, 700, 865
BstYI RGATCY 3 cut(s) 650, 700, 865
BstZI CGGCCG 1 cut(s) 192
BsuRI GGCC 1 cut(s) 194
BtgI CCRYGG 3 cut(s) 60, 541, 720
BtgZI GCGATG 2 cut(s) 303, 929
BtrI CACGTC 1 cut(s) 751
BtsCI GGATG 3 cut(s) 537, 730, 828
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 1 cut(s) 619
BtuMI TCGCGA 2 cut(s) 260, 576
Cac8I GCNNGC 1 cut(s) 405
CfoI GCGC 2 cut(s) 389, 403
Cfr13I GGNCC 2 cut(s) 198, 437
Cfr9I CCCGGG 1 cut(s) 313
CseI GACGC 2 cut(s) 807, 928
Csp6I GTAC 1 cut(s) 781
CviAII CATG 4 cut(s) 123, 218, 384, 778
CviQI GTAC 1 cut(s) 781
DpnI GATC 5 cut(s) 257, 297, 652, 702, 867
DpnII GATC 5 cut(s) 255, 295, 650, 700, 865
DraIII CACNNNGTG 1 cut(s) 721
DriI GACNNNNNGTC 1 cut(s) 204
EaeI YGGCCR 1 cut(s) 192
EagI CGGCCG 1 cut(s) 192
Eam1104I CTCTTC 1 cut(s) 53
Eam1105I GACNNNNNGTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 53
EclXI CGGCCG 1 cut(s) 192
Eco24I GRGCYC 1 cut(s) 601
Eco47I GGWCC 2 cut(s) 198, 437
Eco52I CGGCCG 1 cut(s) 192
Eco72I CACGTG 1 cut(s) 413
Eco88I CYCGRG 2 cut(s) 313, 356
EcoO109I RGGNCCY 2 cut(s) 198, 437
EcoRII CCWGG 2 cut(s) 370, 760
EcoT22I ATGCAT 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 601
Esp3I CGTCTC 2 cut(s) 212, 961
FaeI CATG 4 cut(s) 126, 221, 387, 781
FaiI YATR 8 cut(s) 11, 77, 124, 219, 385, 492, 779, 871
FaqI GGGAC 2 cut(s) 211, 494
FatI CATG 4 cut(s) 122, 217, 383, 777
FauI CCCGC 3 cut(s) 347, 587, 684
Fnu4HI GCNGC 4 cut(s) 192, 687, 690, 738
FokI GGATG 3 cut(s) 524, 737, 815
FriOI GRGCYC 1 cut(s) 601
Fsp4HI GCNGC 4 cut(s) 192, 687, 690, 738
FspBI CTAG 2 cut(s) 398, 806
GlaI GCGC 2 cut(s) 388, 402
GluI GCNGC 4 cut(s) 192, 687, 690, 738
GsuI CTGGAG 1 cut(s) 744
HaeIII GGCC 1 cut(s) 194
HapII CCGG 2 cut(s) 195, 314
HgaI GACGC 2 cut(s) 807, 928
HhaI GCGC 2 cut(s) 389, 403
Hin1I GRCGYC 1 cut(s) 939
Hin1II CATG 4 cut(s) 126, 221, 387, 781
Hin6I GCGC 2 cut(s) 387, 401
HinP1I GCGC 2 cut(s) 387, 401
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 7 cut(s) 305, 474, 479, 612, 642, 681, 757
HpaII CCGG 2 cut(s) 195, 314
HphI GGTGA 1 cut(s) 328
Hpy188I TCNGA 3 cut(s) 500, 637, 823
Hpy99I CGWCG 1 cut(s) 752
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 4 cut(s) 529, 563, 721, 934
HpyCH4IV ACGT 5 cut(s) 412, 450, 750, 970, 999
HpyCH4V TGCA 5 cut(s) 13, 68, 245, 624, 708
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 89, 936, 945
HpySE526I ACGT 5 cut(s) 412, 450, 750, 970, 999
Hsp92I GRCGYC 1 cut(s) 939
Hsp92II CATG 4 cut(s) 126, 221, 387, 781
HspAI GCGC 2 cut(s) 387, 401
KflI GGGWCCC 1 cut(s) 198
Kzo9I GATC 5 cut(s) 255, 295, 650, 700, 865
Lsp1109I GCAGC 1 cut(s) 698
LweI GCATC 4 cut(s) 232, 254, 310, 351
MaeI CTAG 2 cut(s) 398, 806
MaeII ACGT 5 cut(s) 412, 450, 750, 970, 999
MaeIII GTNAC 4 cut(s) 157, 334, 413, 421
MalI GATC 5 cut(s) 257, 297, 652, 702, 867
MbiI CCGCTC 1 cut(s) 692
MboI GATC 5 cut(s) 255, 295, 650, 700, 865
MboII GAAGA 2 cut(s) 40, 563
MflI RGATCY 3 cut(s) 650, 700, 865
MhlI GDGCHC 1 cut(s) 601
MluCI AATT 3 cut(s) 14, 72, 141
MlyI GAGTC 2 cut(s) 636, 751
MmeI TCCRAC 2 cut(s) 199, 660
MnlI CCTC 7 cut(s) 56, 212, 320, 428, 494, 794, 892
Mph1103I ATGCAT 1 cut(s) 247
MseI TTAA 5 cut(s) 30, 590, 744, 837, 1003
MspI CCGG 2 cut(s) 195, 314
MspR9I CCNGG 5 cut(s) 196, 314, 315, 372, 762
Mva1269I GAATGC 1 cut(s) 890
MvaI CCWGG 2 cut(s) 372, 762
MvnI CGCG 6 cut(s) 260, 288, 389, 576, 679, 740
MwoI GCNNNNNNNGC 4 cut(s) 65, 89, 936, 945
NciI CCSGG 3 cut(s) 196, 314, 315
NdeII GATC 5 cut(s) 255, 295, 650, 700, 865
NlaIII CATG 4 cut(s) 126, 221, 387, 781
NlaIV GGNNCC 3 cut(s) 199, 200, 652
NmuCI GTSAC 3 cut(s) 334, 413, 421
NruI TCGCGA 2 cut(s) 260, 576
NsiI ATGCAT 1 cut(s) 247
NspI RCATGY 2 cut(s) 126, 781
NspV TTCGAA 1 cut(s) 477
PaeR7I CTCGAG 1 cut(s) 356
PciI ACATGT 2 cut(s) 122, 777
PcsI WCGNNNNNNNCGW 1 cut(s) 879
PctI GAATGC 1 cut(s) 890
PfeI GAWTC 5 cut(s) 305, 474, 479, 612, 681
PflFI GACNNNGTC 2 cut(s) 332, 419
PflMI CCANNNNNTGG 1 cut(s) 721
PfoI TCCNGGA 1 cut(s) 760
PkrI GCNGC 4 cut(s) 193, 688, 691, 739
PleI GAGTC 2 cut(s) 636, 751
PmaCI CACGTG 1 cut(s) 413
PmlI CACGTG 1 cut(s) 413
PpsI GAGTC 2 cut(s) 636, 751
Ppu21I YACGTR 1 cut(s) 413
PpuMI RGGWCCY 2 cut(s) 198, 437
PscI ACATGT 2 cut(s) 122, 777
Psp1406I AACGTT 1 cut(s) 999
Psp5II RGGWCCY 2 cut(s) 198, 437
Psp6I CCWGG 2 cut(s) 370, 760
PspCI CACGTG 1 cut(s) 413
PspGI CCWGG 2 cut(s) 370, 760
PspN4I GGNNCC 3 cut(s) 199, 200, 652
PspPI GGNCC 2 cut(s) 198, 437
PspPPI RGGWCCY 2 cut(s) 198, 437
PsuI RGATCY 3 cut(s) 650, 700, 865
PsyI GACNNNGTC 2 cut(s) 332, 419
RruI TCGCGA 2 cut(s) 260, 576
RsaI GTAC 1 cut(s) 782
RsaNI GTAC 1 cut(s) 781
SaqAI TTAA 5 cut(s) 30, 590, 744, 837, 1003
SatI GCNGC 4 cut(s) 192, 687, 690, 738
Sau3AI GATC 5 cut(s) 255, 295, 650, 700, 865
Sau96I GGNCC 2 cut(s) 198, 437
SchI GAGTC 2 cut(s) 636, 751
ScrFI CCNGG 5 cut(s) 196, 314, 315, 372, 762
SduI GDGCHC 1 cut(s) 601
SfaNI GCATC 4 cut(s) 232, 254, 310, 351
Sfr274I CTCGAG 1 cut(s) 356
SfuI TTCGAA 1 cut(s) 477
SinI GGWCC 2 cut(s) 198, 437
SlaI CTCGAG 1 cut(s) 356
SmaI CCCGGG 1 cut(s) 315
SmlI CTYRAG 2 cut(s) 97, 356
SmoI CTYRAG 2 cut(s) 97, 356
Sse9I AATT 3 cut(s) 14, 72, 141
SsiI CCGC 8 cut(s) 191, 281, 340, 389, 594, 677, 690, 738
SspMI CTAG 2 cut(s) 398, 806
StyD4I CCNGG 5 cut(s) 194, 312, 313, 370, 760
TaaI ACNGT 4 cut(s) 529, 563, 721, 934
TaiI ACGT 5 cut(s) 415, 453, 753, 973, 1002
TaqI TCGA 4 cut(s) 5, 357, 477, 771
TasI AATT 3 cut(s) 14, 72, 141
TatI WGTACW 1 cut(s) 780
TauI GCSGC 3 cut(s) 194, 692, 740
TfiI GAWTC 5 cut(s) 305, 474, 479, 612, 681
Tru1I TTAA 5 cut(s) 30, 590, 744, 837, 1003
Tru9I TTAA 5 cut(s) 30, 590, 744, 837, 1003
TscAI CASTG 1 cut(s) 626
TseFI GTSAC 3 cut(s) 334, 413, 421
TseI GCWGC 1 cut(s) 686
Tsp45I GTSAC 3 cut(s) 334, 413, 421
TspGWI ACGGA 4 cut(s) 119, 194, 486, 663
TspMI CCCGGG 1 cut(s) 313
TspRI CASTG 1 cut(s) 626
Tth111I GACNNNGTC 2 cut(s) 332, 419
Van91I CCANNNNNTGG 1 cut(s) 721
VpaK11BI GGWCC 2 cut(s) 198, 437
XceI RCATGY 2 cut(s) 126, 781
XcmI CCANNNNNNNNNTGG 1 cut(s) 859
XhoI CTCGAG 1 cut(s) 356
XmaI CCCGGG 1 cut(s) 313
XspI CTAG 2 cut(s) 398, 806
Zsp2I ATGCAT 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.