MD06G1180500.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003752
Physical Location & Seq
Forward (+)
32013448 .. 32014425
978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1180500.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGCTATTCCTATACTGCTGCTTCTCTTCTTCTCCATTCCCTTCTCCGAATCGCAACTCACCAAGGATTACTACAAGCAAACATGTCCAGACTTTGAGAAGATTGTCAGAGACACCGCGTCCCAAAAGCAAGCCGCCCAACCCACCACCGCTGCAGGAACTCTCCGCCTCTTCTTCCACGACTGCATGGTTGAAGGATGTGACGCCTCCGTTCTCATTGCCTCCAACCATGTCAACAAAGCTGAGCGCGATGCTGACATCAACCAATCCCTGTCAGGAGATGCGCTTGAGGTGGTAGTCCGCGCCAAGACCGCCCTAGAGCTTACCTGCCCGGGGATTGTCTCGTGCGCAGACATTCTCGCCGAGGCCACACGTGACCTTGTCACGATGGTTGGTGGGCCTTTCTACTTCGTCGAGTTGGGACGAAAAGATGGACAGGTTTCCCTTGCCTCCAAGGTTAACGCAAACTTGCCAAGAGCAAACCAAACCATGGATGACATCATCAAATTCTTCGCCGCTAAGGGTTTCACAATTGAAGAAATGGTTGCTTTAAGTGGTGGACACACAATTGGGTTCTCTCATTGCATCGAGTTCACTGACAGGCTTTTCCATTACAGCAAGACTACTCCAACAGACCCAGAAATCAACCCTAGATTCGCCGAAGGCTTGAAGATGACATGCGCTAACTACACAACTAATCCTGCCATGTCCGCCTTCAACGACGTGATTACACCCGGAAAATTCGATAACATGTACTATCAAAATCTGAAGAGGGGTTTGGGGCTGTTGGCATCGGACCATGCACTTGTTAAGGACCCGAGGACGAAGCCTCTTGTGGAGTTGTACTCTACTGACCAGGCGGCATTTTTCAAGGCTTTCTCTCTTGCAATGGAGAAGCTTGGACGTCATGAGATTAAGACCGGGAATCAGGGAGAGGTGAGGCGCAGGTGCGACACGTTTAACTCGCTTCAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0009044 GO:0016787 GO:0016798 GO:0097599
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

326

Amino Acids

35.87

Weight (kDa)

6.0

Isoelectric Point (pI)

33.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 36 - 284 1.9e-77 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 936
AatII GACGTC 1 cut(s) 907
Acc16I TGCGCA 1 cut(s) 349
Acc36I ACCTGC 2 cut(s) 335, 936
AccB7I CCANNNNNTGG 1 cut(s) 490
AccII CGCG 3 cut(s) 119, 249, 303
AciI CCGC 9 cut(s) 117, 135, 150, 166, 301, 312, 516, 711, 860
AcsI RAATTY 2 cut(s) 506, 740
AcuI CTGAAG 2 cut(s) 788, 953
AcvI CACGTG 1 cut(s) 374
AcyI GRCGYC 2 cut(s) 204, 904
AfaI GTAC 2 cut(s) 755, 845
AfiI CCNNNNNNNGG 2 cut(s) 333, 490
AflIII ACRYGT 4 cut(s) 83, 371, 750, 954
AgsI TTSAA 5 cut(s) 194, 536, 670, 718, 871
AhdI GACNNNNNGTC 1 cut(s) 118
AjiI CACGTC 1 cut(s) 724
AjnI CCWGG 1 cut(s) 855
AjuI GAANNNNNNNTTGG 2 cut(s) 761, 793
AluBI AGCT 3 cut(s) 242, 322, 898
AluI AGCT 3 cut(s) 242, 322, 898
Alw26I GTCTC 2 cut(s) 105, 346
Ama87I CYCGRG 2 cut(s) 331, 817
AoxI GGCC 2 cut(s) 366, 398
ApeKI GCWGC 2 cut(s) 19, 152
ApoI RAATTY 2 cut(s) 506, 740
AspLEI GCGC 6 cut(s) 249, 286, 305, 350, 683, 945
AspS9I GGNCC 3 cut(s) 398, 796, 814
AsuC2I CCSGG 4 cut(s) 332, 333, 735, 922
AsuHPI GGTGA 2 cut(s) 52, 949
AvaI CYCGRG 2 cut(s) 331, 817
AvaII GGWCC 2 cut(s) 796, 814
BauI CACGAG 1 cut(s) 343
BbrPI CACGTG 1 cut(s) 374
BbvI GCAGC 2 cut(s) 6, 139
BccI CCATC 2 cut(s) 382, 425
BciT130I CCWGG 1 cut(s) 857
BcnI CCSGG 4 cut(s) 332, 333, 735, 922
BcoDI GTCTC 2 cut(s) 105, 346
BfaI CTAG 2 cut(s) 317, 651
BfmI CTRYAG 1 cut(s) 153
BfuAI ACCTGC 2 cut(s) 335, 936
BisI GCNGC 5 cut(s) 20, 135, 153, 516, 861
BlpI GCTNAGC 1 cut(s) 243
BlsI GCNGC 5 cut(s) 21, 136, 154, 517, 862
Bme1390I CCNGG 5 cut(s) 332, 333, 735, 857, 922
Bme18I GGWCC 2 cut(s) 796, 814
BmeRI GACNNNNNGTC 1 cut(s) 118
BmeT110I CYCGRG 2 cut(s) 331, 817
BmgBI CACGTC 1 cut(s) 724
BmgT120I GGNCC 3 cut(s) 398, 796, 814
BmiI GGNNCC 1 cut(s) 816
BmrFI CCNGG 5 cut(s) 332, 333, 735, 857, 922
BmsI GCATC 4 cut(s) 241, 271, 594, 800
BplI GAGNNNNNCTC 2 cut(s) 830, 862
Bpu10I CCTNAGC 1 cut(s) 519
Bpu1102I GCTNAGC 1 cut(s) 243
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 4 cut(s) 332, 333, 735, 922
BsaAI YACGTR 1 cut(s) 374
BsaHI GRCGYC 2 cut(s) 204, 904
BsaJI CCNNGG 7 cut(s) 63, 331, 332, 363, 453, 489, 818
Bsc4I CCNNNNNNNGG 2 cut(s) 333, 490
Bse3DI GCAATG 3 cut(s) 216, 580, 894
BseBI CCWGG 1 cut(s) 857
BseDI CCNNGG 7 cut(s) 63, 331, 332, 363, 453, 489, 818
BseGI GGATG 2 cut(s) 203, 499
BseLI CCNNNNNNNGG 2 cut(s) 333, 490
BseMI GCAATG 3 cut(s) 216, 580, 894
BseMII CTCAG 1 cut(s) 234
BseXI GCAGC 2 cut(s) 6, 139
Bsh1236I CGCG 3 cut(s) 119, 249, 303
BshFI GGCC 2 cut(s) 368, 400
BsiHKCI CYCGRG 2 cut(s) 331, 817
BsiSI CCGG 3 cut(s) 332, 735, 921
BslFI GGGAC 2 cut(s) 106, 435
BslI CCNNNNNNNGG 2 cut(s) 333, 490
BsmAI GTCTC 2 cut(s) 105, 346
BsmFI GGGAC 2 cut(s) 106, 435
BsnI GGCC 2 cut(s) 368, 400
BsoBI CYCGRG 2 cut(s) 331, 817
Bsp1720I GCTNAGC 1 cut(s) 243
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 9 cut(s) 117, 135, 150, 166, 301, 312, 516, 711, 860
BspANI GGCC 2 cut(s) 368, 400
BspCNI CTCAG 1 cut(s) 235
BspFNI CGCG 3 cut(s) 119, 249, 303
BspHI TCATGA 1 cut(s) 907
BspLI GGNNCC 1 cut(s) 816
BspMAI CTGCAG 1 cut(s) 157
BspMI ACCTGC 2 cut(s) 335, 936
BsrDI GCAATG 3 cut(s) 216, 580, 894
BssECI CCNNGG 7 cut(s) 63, 331, 332, 363, 453, 489, 818
BssNI GRCGYC 2 cut(s) 204, 904
BssSI CACGAG 1 cut(s) 343
BssT1I CCWWGG 3 cut(s) 63, 453, 489
Bst2BI CACGAG 1 cut(s) 343
Bst2UI CCWGG 1 cut(s) 857
Bst6I CTCTTC 3 cut(s) 32, 176, 764
BstACI GRCGYC 2 cut(s) 204, 904
BstBAI YACGTR 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 132
BstDEI CTNAG 3 cut(s) 243, 519, 975
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 2 cut(s) 203, 499
BstFNI CGCG 3 cut(s) 119, 249, 303
BstHHI GCGC 6 cut(s) 249, 286, 305, 350, 683, 945
BstMAI GTCTC 2 cut(s) 105, 346
BstMWI GCNNNNNNNGC 2 cut(s) 311, 710
BstNI CCWGG 1 cut(s) 857
BstNSI RCATGY 3 cut(s) 87, 681, 754
BstSCI CCNGG 5 cut(s) 330, 331, 733, 855, 920
BstSFI CTRYAG 1 cut(s) 153
BstUI CGCG 3 cut(s) 119, 249, 303
BstV1I GCAGC 2 cut(s) 6, 139
BsuRI GGCC 2 cut(s) 368, 400
BtgI CCRYGG 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 264
BtrI CACGTC 1 cut(s) 724
BtsCI GGATG 2 cut(s) 203, 499
BtsIMutI CAGTG 1 cut(s) 594
BveI ACCTGC 2 cut(s) 335, 936
Cac8I GCNNGC 1 cut(s) 132
CciI TCATGA 1 cut(s) 907
CfoI GCGC 6 cut(s) 249, 286, 305, 350, 683, 945
Cfr13I GGNCC 3 cut(s) 398, 796, 814
Cfr9I CCCGGG 1 cut(s) 331
CseI GACGC 2 cut(s) 108, 212
Csp6I GTAC 2 cut(s) 754, 844
CviAII CATG 9 cut(s) 84, 187, 230, 490, 678, 706, 751, 800, 908
CviQI GTAC 2 cut(s) 754, 844
DdeI CTNAG 3 cut(s) 243, 519, 975
DriI GACNNNNNGTC 1 cut(s) 118
Eam1104I CTCTTC 3 cut(s) 32, 176, 764
Eam1105I GACNNNNNGTC 1 cut(s) 118
EarI CTCTTC 3 cut(s) 32, 176, 764
EciI GGCGGA 2 cut(s) 155, 700
Eco130I CCWWGG 3 cut(s) 63, 453, 489
Eco47I GGWCC 2 cut(s) 796, 814
Eco57I CTGAAG 2 cut(s) 788, 953
Eco72I CACGTG 1 cut(s) 374
Eco88I CYCGRG 2 cut(s) 331, 817
EcoO109I RGGNCCY 1 cut(s) 814
EcoRII CCWGG 1 cut(s) 855
EcoT14I CCWWGG 3 cut(s) 63, 453, 489
ErhI CCWWGG 3 cut(s) 63, 453, 489
FaeI CATG 9 cut(s) 87, 190, 233, 493, 681, 709, 754, 803, 911
FaqI GGGAC 2 cut(s) 106, 435
FatI CATG 9 cut(s) 83, 186, 229, 489, 677, 705, 750, 799, 907
Fnu4HI GCNGC 5 cut(s) 20, 135, 153, 516, 861
FokI GGATG 2 cut(s) 210, 506
Fsp4HI GCNGC 5 cut(s) 20, 135, 153, 516, 861
FspBI CTAG 2 cut(s) 317, 651
FspI TGCGCA 1 cut(s) 349
GlaI GCGC 6 cut(s) 248, 285, 304, 349, 682, 944
GluI GCNGC 5 cut(s) 20, 135, 153, 516, 861
HaeIII GGCC 2 cut(s) 368, 400
HapII CCGG 3 cut(s) 332, 735, 921
HgaI GACGC 2 cut(s) 108, 212
HhaI GCGC 6 cut(s) 249, 286, 305, 350, 683, 945
Hin1I GRCGYC 2 cut(s) 204, 904
Hin1II CATG 9 cut(s) 87, 190, 233, 493, 681, 709, 754, 803, 911
Hin6I GCGC 6 cut(s) 247, 284, 303, 348, 681, 943
HinP1I GCGC 6 cut(s) 247, 284, 303, 348, 681, 943
HincII GTYRAC 2 cut(s) 235, 460
HindII GTYRAC 2 cut(s) 235, 460
HindIII AAGCTT 1 cut(s) 896
HinfI GANTC 3 cut(s) 50, 654, 925
HpaI GTTAAC 1 cut(s) 460
HpaII CCGG 3 cut(s) 332, 735, 921
HphI GGTGA 2 cut(s) 52, 949
Hpy166II GTNNAC 4 cut(s) 235, 460, 560, 594
Hpy188I TCNGA 4 cut(s) 49, 110, 768, 796
Hpy188III TCNNGA 4 cut(s) 89, 276, 385, 908
Hpy8I GTNNAC 4 cut(s) 235, 460, 560, 594
Hpy99I CGWCG 2 cut(s) 416, 725
HpyAV CCTTC 4 cut(s) 52, 188, 656, 724
HpyCH4IV ACGT 4 cut(s) 373, 723, 904, 956
HpyCH4V TGCA 5 cut(s) 155, 186, 585, 803, 887
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 710
HpyF3I CTNAG 3 cut(s) 243, 519, 975
HpySE526I ACGT 4 cut(s) 373, 723, 904, 956
Hsp92I GRCGYC 2 cut(s) 204, 904
Hsp92II CATG 9 cut(s) 87, 190, 233, 493, 681, 709, 754, 803, 911
HspAI GCGC 6 cut(s) 247, 284, 303, 348, 681, 943
KspAI GTTAAC 1 cut(s) 460
Lsp1109I GCAGC 2 cut(s) 6, 139
LweI GCATC 4 cut(s) 241, 271, 594, 800
MaeI CTAG 2 cut(s) 317, 651
MaeII ACGT 4 cut(s) 373, 723, 904, 956
MaeIII GTNAC 3 cut(s) 200, 374, 382
MboII GAAGA 9 cut(s) 19, 22, 112, 163, 166, 502, 548, 682, 781
MfeI CAATTG 2 cut(s) 531, 567
MluCI AATT 4 cut(s) 506, 531, 567, 740
MmeI TCCRAC 2 cut(s) 249, 653
MseI TTAA 5 cut(s) 459, 551, 810, 915, 960
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 3 cut(s) 332, 735, 921
MspR9I CCNGG 5 cut(s) 332, 333, 735, 857, 922
MunI CAATTG 2 cut(s) 531, 567
MvaI CCWGG 1 cut(s) 857
MvnI CGCG 3 cut(s) 119, 249, 303
MwoI GCNNNNNNNGC 2 cut(s) 311, 710
NciI CCSGG 4 cut(s) 332, 333, 735, 922
NcoI CCATGG 1 cut(s) 489
NlaIII CATG 9 cut(s) 87, 190, 233, 493, 681, 709, 754, 803, 911
NlaIV GGNNCC 1 cut(s) 816
NmeAIII GCCGAG 1 cut(s) 388
NmuCI GTSAC 3 cut(s) 200, 374, 382
NsbI TGCGCA 1 cut(s) 349
NspI RCATGY 3 cut(s) 87, 681, 754
PagI TCATGA 1 cut(s) 907
PaqCI CACCTGC 1 cut(s) 936
PciI ACATGT 2 cut(s) 83, 750
PfeI GAWTC 3 cut(s) 50, 654, 925
PflFI GACNNNGTC 1 cut(s) 380
PflMI CCANNNNNTGG 1 cut(s) 490
PkrI GCNGC 5 cut(s) 21, 136, 154, 517, 862
PmaCI CACGTG 1 cut(s) 374
PmlI CACGTG 1 cut(s) 374
Ppu21I YACGTR 1 cut(s) 374
PpuMI RGGWCCY 1 cut(s) 814
PscI ACATGT 2 cut(s) 83, 750
Psp5II RGGWCCY 1 cut(s) 814
Psp6I CCWGG 1 cut(s) 855
PspCI CACGTG 1 cut(s) 374
PspGI CCWGG 1 cut(s) 855
PspN4I GGNNCC 1 cut(s) 816
PspPI GGNCC 3 cut(s) 398, 796, 814
PspPPI RGGWCCY 1 cut(s) 814
PstI CTGCAG 1 cut(s) 157
PsyI GACNNNGTC 1 cut(s) 380
RsaI GTAC 2 cut(s) 755, 845
RsaNI GTAC 2 cut(s) 754, 844
SaqAI TTAA 5 cut(s) 459, 551, 810, 915, 960
SatI GCNGC 5 cut(s) 20, 135, 153, 516, 861
Sau96I GGNCC 3 cut(s) 398, 796, 814
ScrFI CCNGG 5 cut(s) 332, 333, 735, 857, 922
SfaNI GCATC 4 cut(s) 241, 271, 594, 800
SfcI CTRYAG 1 cut(s) 153
SinI GGWCC 2 cut(s) 796, 814
SmaI CCCGGG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 4 cut(s) 506, 531, 567, 740
SsiI CCGC 9 cut(s) 117, 135, 150, 166, 301, 312, 516, 711, 860
SspMI CTAG 2 cut(s) 317, 651
StyD4I CCNGG 5 cut(s) 330, 331, 733, 855, 920
StyI CCWWGG 3 cut(s) 63, 453, 489
TaiI ACGT 4 cut(s) 376, 726, 907, 959
TaqI TCGA 3 cut(s) 414, 588, 744
TasI AATT 4 cut(s) 506, 531, 567, 740
TatI WGTACW 2 cut(s) 753, 843
TauI GCSGC 3 cut(s) 137, 518, 863
TfiI GAWTC 3 cut(s) 50, 654, 925
Tru1I TTAA 5 cut(s) 459, 551, 810, 915, 960
Tru9I TTAA 5 cut(s) 459, 551, 810, 915, 960
TscAI CASTG 1 cut(s) 601
TseFI GTSAC 3 cut(s) 200, 374, 382
TseI GCWGC 2 cut(s) 19, 152
Tsp45I GTSAC 3 cut(s) 200, 374, 382
TspGWI ACGGA 1 cut(s) 199
TspMI CCCGGG 1 cut(s) 331
TspRI CASTG 1 cut(s) 601
Tth111I GACNNNGTC 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 490
VpaK11BI GGWCC 2 cut(s) 796, 814
XapI RAATTY 2 cut(s) 506, 740
XceI RCATGY 3 cut(s) 87, 681, 754
XmaI CCCGGG 1 cut(s) 331
XspI CTAG 2 cut(s) 317, 651
ZraI GACGTC 1 cut(s) 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.