MD06G1180700.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003752
Physical Location & Seq
Forward (+)
32016607 .. 32017584
978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1180700.v1.1.491

Sequence Viewer

Length: 978 bp
ATGGCTCTTCCAATCCTCTTCCTACTCTTCCTCGCCATTCCCTTCTCCGAATCCAAGCTCTCCATAGACTACTACAAAAAAACATGCCCCGACTTCGAGAGCATTGTCCGTGACACCGTGACTTCAAAACAAATCGCCAGTCCCACCACCGCGGCAGGCACTCTCCGCCTCTTCTTCCACGACTGCATGGTTGAAGGCTGCGATGCTTCCATCCTCATCTCCTCCAACTACGTTAACACTGCAGAGCGTGACGCCGACATCAACCAATCCCTCTCGGCAGACGCCTTTGACCTCGTGGCACGTGCCAAGACCGCCCTCGAGCTCGCCTGCCCCGGCATTGTCTCCTGCTCCGATATACTCGCCTTAGCCACCCGCAACCTCGTCACGATGGTCGGCGGTCCGTTCTACAAAGTCCGCTTGGGACGGAAAGACGGACAGGTTTCCAAATCTTCACTAGTGGAAGGAAATCTCCCAAGAAGCAACCAAACCCTAGACGACATGATCAAATTCTTCGCCACAAAAGGCTTCACACTCCAAGAAATGGTGGCTTTATCCGGTGCACACACAATTGGATTCTCTCACTGCAAAGAATTCGCCGACCGGATTTTCAATTACAACAAAACTACACCAACAGATCCTGACATGTACCCAAACTATGCTCAAGGGCTCAAGAAAATTTGTGCAGACTACAAAACGAACATCGCCATGTCCGCTTTCAACGATGTGATTACGCCCGGCAAGTTCGATAACATGTATTATCAGAATCTGAAGAGAGGGTTGGGGTTGTTACCTACGGATCATGCACTTGTTAAGGACCCGAGGACGAGACCTTTTGTGGAGTTGTACTCGACGGACCAGGCTGCCTTTTTCCAAGCTTTTTCTCATGCTATGGAGAAGCTTAGCCATCATGGAATTAAGACTGGACGTAAAGGTGAGGTCAGGCATAGGTGTGATGCGTTTAACTCGATTAACGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004553 GO:0009044 GO:0016787 GO:0016798 GO:0097599
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

326

Amino Acids

36.0

Weight (kDa)

8.05

Isoelectric Point (pI)

31.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 36 - 284 1e-78 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 541
AccII CGCG 1 cut(s) 152
AciI CCGC 8 cut(s) 150, 152, 166, 312, 373, 396, 415, 711
AclWI GGATC 2 cut(s) 629, 804
AcsI RAATTY 3 cut(s) 506, 590, 675
AcuI CTGAAG 1 cut(s) 788
AcvI CACGTG 1 cut(s) 302
AcyI GRCGYC 2 cut(s) 252, 282
AfaI GTAC 2 cut(s) 647, 845
AfiI CCNNNNNNNGG 1 cut(s) 541
AflIII ACRYGT 2 cut(s) 642, 750
AgsI TTSAA 4 cut(s) 126, 194, 610, 718
AhlI ACTAGT 1 cut(s) 454
AjnI CCWGG 1 cut(s) 855
AjuI GAANNNNNNNTTGG 2 cut(s) 761, 793
AluBI AGCT 4 cut(s) 58, 322, 875, 898
AluI AGCT 4 cut(s) 58, 322, 875, 898
Alw21I GWGCWC 2 cut(s) 324, 562
Alw26I GTCTC 2 cut(s) 346, 820
Alw44I GTGCAC 1 cut(s) 558
AlwI GGATC 2 cut(s) 629, 804
AlwNI CAGNNNCTG 2 cut(s) 638, 766
Ama87I CYCGRG 2 cut(s) 317, 817
ApaLI GTGCAC 1 cut(s) 558
ApeKI GCWGC 2 cut(s) 198, 860
ApoI RAATTY 3 cut(s) 506, 590, 675
ArsI GACNNNNNNTTYG 2 cut(s) 124, 156
AspS9I GGNCC 3 cut(s) 398, 814, 853
AsuC2I CCSGG 2 cut(s) 333, 735
AsuHPI GGTGA 1 cut(s) 944
AvaI CYCGRG 2 cut(s) 317, 817
AvaII GGWCC 3 cut(s) 398, 814, 853
BaeGI GKGCMC 1 cut(s) 562
BanII GRGCYC 2 cut(s) 324, 669
BauI CACGAG 1 cut(s) 293
BbrPI CACGTG 1 cut(s) 302
Bbv12I GWGCWC 2 cut(s) 324, 562
BbvI GCAGC 2 cut(s) 185, 847
BccI CCATC 3 cut(s) 218, 382, 912
BcgI CGANNNNNNTGC 2 cut(s) 574, 608
BciT130I CCWGG 1 cut(s) 857
BclI TGATCA 1 cut(s) 501
BcnI CCSGG 2 cut(s) 333, 735
BcoDI GTCTC 2 cut(s) 346, 820
BcuI ACTAGT 1 cut(s) 454
BfaI CTAG 2 cut(s) 455, 491
BfmI CTRYAG 1 cut(s) 240
BisI GCNGC 3 cut(s) 153, 199, 861
BlpI GCTNAGC 1 cut(s) 899
BlsI GCNGC 3 cut(s) 154, 200, 862
Bme1390I CCNGG 3 cut(s) 333, 735, 857
Bme18I GGWCC 3 cut(s) 398, 814, 853
BmeT110I CYCGRG 2 cut(s) 317, 817
BmgT120I GGNCC 3 cut(s) 398, 814, 853
BmiI GGNNCC 1 cut(s) 816
BmrFI CCNGG 3 cut(s) 333, 735, 857
BmsI GCATC 2 cut(s) 193, 943
BplI GAGNNNNNCTC 2 cut(s) 830, 862
Bpu10I CCTNAGC 1 cut(s) 364
Bpu1102I GCTNAGC 1 cut(s) 899
BpuEI CTTGAG 2 cut(s) 645, 653
BpuMI CCSGG 2 cut(s) 333, 735
BsaAI YACGTR 1 cut(s) 302
BsaHI GRCGYC 2 cut(s) 252, 282
BsaI GGTCTC 1 cut(s) 820
BsaJI CCNNGG 3 cut(s) 150, 331, 818
BsaWI WCCGGW 2 cut(s) 554, 600
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse1I ACTGG 2 cut(s) 138, 925
BseBI CCWGG 1 cut(s) 857
BseDI CCNNGG 3 cut(s) 150, 331, 818
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 541
BseNI ACTGG 2 cut(s) 138, 925
BseRI GAGGAG 1 cut(s) 211
BseSI GKGCMC 1 cut(s) 562
BseXI GCAGC 2 cut(s) 185, 847
BsgI GTGCAG 1 cut(s) 702
Bsh1236I CGCG 1 cut(s) 152
Bsh1285I CGRYCG 1 cut(s) 601
BsiEI CGRYCG 1 cut(s) 601
BsiHKAI GWGCWC 2 cut(s) 324, 562
BsiHKCI CYCGRG 2 cut(s) 317, 817
BsiSI CCGG 4 cut(s) 333, 555, 601, 735
BslFI GGGAC 2 cut(s) 126, 435
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 2 cut(s) 346, 820
BsmFI GGGAC 2 cut(s) 126, 435
Bso31I GGTCTC 1 cut(s) 820
BsoBI CYCGRG 2 cut(s) 317, 817
Bsp1286I GDGCHC 3 cut(s) 324, 562, 669
Bsp143I GATC 3 cut(s) 501, 634, 796
Bsp1720I GCTNAGC 1 cut(s) 899
BspACI CCGC 8 cut(s) 150, 152, 166, 312, 373, 396, 415, 711
BspFNI CGCG 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 816
BspMAI CTGCAG 1 cut(s) 244
BspPI GGATC 2 cut(s) 629, 804
BspQI GCTCTTC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 820
BsrI ACTGG 2 cut(s) 138, 925
BssECI CCNNGG 3 cut(s) 150, 331, 818
BssMI GATC 3 cut(s) 501, 634, 796
BssNI GRCGYC 2 cut(s) 252, 282
BssSI CACGAG 1 cut(s) 293
Bst2BI CACGAG 1 cut(s) 293
Bst2UI CCWGG 1 cut(s) 857
Bst4CI ACNGT 1 cut(s) 118
Bst6I CTCTTC 5 cut(s) 12, 23, 32, 176, 764
BstACI GRCGYC 2 cut(s) 252, 282
BstBAI YACGTR 1 cut(s) 302
BstC8I GCNNGC 3 cut(s) 157, 324, 328
BstDEI CTNAG 2 cut(s) 364, 899
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 1 cut(s) 210
BstFNI CGCG 1 cut(s) 152
BstKTI GATC 3 cut(s) 504, 637, 799
BstMAI GTCTC 2 cut(s) 346, 820
BstMBI GATC 3 cut(s) 501, 634, 796
BstMCI CGRYCG 1 cut(s) 601
BstMWI GCNNNNNNNGC 3 cut(s) 165, 311, 710
BstNI CCWGG 1 cut(s) 857
BstNSI RCATGY 3 cut(s) 87, 646, 754
BstSCI CCNGG 3 cut(s) 331, 733, 855
BstSFI CTRYAG 1 cut(s) 240
BstSLI GKGCMC 1 cut(s) 562
BstUI CGCG 1 cut(s) 152
BstV1I GCAGC 2 cut(s) 185, 847
BstX2I RGATCY 1 cut(s) 634
BstYI RGATCY 1 cut(s) 634
BtgI CCRYGG 1 cut(s) 150
BtgZI GCGATG 2 cut(s) 216, 685
BtsCI GGATG 1 cut(s) 210
BtsI GCAGTG 2 cut(s) 237, 580
BtsIMutI CAGTG 2 cut(s) 237, 580
Cac8I GCNNGC 3 cut(s) 157, 324, 328
CaiI CAGNNNCTG 2 cut(s) 638, 766
Cfr13I GGNCC 3 cut(s) 398, 814, 853
Cfr42I CCGCGG 1 cut(s) 153
CpoI CGGWCCG 1 cut(s) 398
CseI GACGC 2 cut(s) 260, 290
Csp6I GTAC 2 cut(s) 646, 844
CspI CGGWCCG 1 cut(s) 398
CviAII CATG 9 cut(s) 84, 187, 499, 643, 706, 751, 800, 884, 908
CviQI GTAC 2 cut(s) 646, 844
DdeI CTNAG 2 cut(s) 364, 899
DpnI GATC 3 cut(s) 503, 636, 798
DpnII GATC 3 cut(s) 501, 634, 796
Eam1104I CTCTTC 5 cut(s) 12, 23, 32, 176, 764
EarI CTCTTC 5 cut(s) 12, 23, 32, 176, 764
EciI GGCGGA 1 cut(s) 155
Ecl136II GAGCTC 1 cut(s) 322
Eco24I GRGCYC 2 cut(s) 324, 669
Eco31I GGTCTC 1 cut(s) 820
Eco47I GGWCC 3 cut(s) 398, 814, 853
Eco53kI GAGCTC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 788
Eco72I CACGTG 1 cut(s) 302
Eco88I CYCGRG 2 cut(s) 317, 817
EcoICRI GAGCTC 1 cut(s) 322
EcoO109I RGGNCCY 1 cut(s) 814
EcoRI GAATTC 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 855
EcoT38I GRGCYC 2 cut(s) 324, 669
FaeI CATG 9 cut(s) 87, 190, 502, 646, 709, 754, 803, 887, 911
FaqI GGGAC 2 cut(s) 126, 435
FatI CATG 9 cut(s) 83, 186, 498, 642, 705, 750, 799, 883, 907
FauI CCCGC 1 cut(s) 380
FbaI TGATCA 1 cut(s) 501
Fnu4HI GCNGC 3 cut(s) 153, 199, 861
FokI GGATG 1 cut(s) 197
FriOI GRGCYC 2 cut(s) 324, 669
Fsp4HI GCNGC 3 cut(s) 153, 199, 861
FspBI CTAG 2 cut(s) 455, 491
GluI GCNGC 3 cut(s) 153, 199, 861
HapII CCGG 4 cut(s) 333, 555, 601, 735
HgaI GACGC 2 cut(s) 260, 290
Hin1I GRCGYC 2 cut(s) 252, 282
Hin1II CATG 9 cut(s) 87, 190, 502, 646, 709, 754, 803, 887, 911
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 2 cut(s) 873, 896
HinfI GANTC 3 cut(s) 50, 573, 763
HpaI GTTAAC 1 cut(s) 235
HpaII CCGG 4 cut(s) 333, 555, 601, 735
HphI GGTGA 1 cut(s) 944
Hpy166II GTNNAC 2 cut(s) 235, 560
Hpy188I TCNGA 4 cut(s) 49, 352, 762, 768
Hpy188III TCNNGA 4 cut(s) 97, 385, 638, 670
Hpy8I GTNNAC 2 cut(s) 235, 560
Hpy99I CGWCG 1 cut(s) 853
HpyAV CCTTC 3 cut(s) 52, 188, 455
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4IV ACGT 3 cut(s) 231, 301, 925
HpyCH4V TGCA 6 cut(s) 186, 242, 560, 585, 683, 803
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 311, 710
HpyF3I CTNAG 2 cut(s) 364, 899
HpySE526I ACGT 3 cut(s) 231, 301, 925
Hsp92I GRCGYC 2 cut(s) 252, 282
Hsp92II CATG 9 cut(s) 87, 190, 502, 646, 709, 754, 803, 887, 911
Ksp22I TGATCA 1 cut(s) 501
KspAI GTTAAC 1 cut(s) 235
KspI CCGCGG 1 cut(s) 153
Kzo9I GATC 3 cut(s) 501, 634, 796
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 353
Lsp1109I GCAGC 2 cut(s) 185, 847
LweI GCATC 2 cut(s) 193, 943
MaeI CTAG 2 cut(s) 455, 491
MaeII ACGT 3 cut(s) 231, 301, 925
MaeIII GTNAC 5 cut(s) 110, 118, 248, 382, 786
MalI GATC 3 cut(s) 503, 636, 798
MboI GATC 3 cut(s) 501, 634, 796
MboII GAAGA 7 cut(s) 10, 19, 163, 166, 441, 502, 781
MfeI CAATTG 1 cut(s) 567
MflI RGATCY 1 cut(s) 634
MhlI GDGCHC 3 cut(s) 324, 562, 669
MluCI AATT 6 cut(s) 506, 567, 590, 610, 675, 912
MmeI TCCRAC 1 cut(s) 249
MseI TTAA 6 cut(s) 234, 810, 915, 960, 969, 976
MslI CAYNNNNRTG 2 cut(s) 704, 948
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 4 cut(s) 333, 555, 601, 735
MspR9I CCNGG 3 cut(s) 333, 735, 857
MunI CAATTG 1 cut(s) 567
MvaI CCWGG 1 cut(s) 857
MvnI CGCG 1 cut(s) 152
MwoI GCNNNNNNNGC 3 cut(s) 165, 311, 710
NciI CCSGG 2 cut(s) 333, 735
NdeII GATC 3 cut(s) 501, 634, 796
NlaIII CATG 9 cut(s) 87, 190, 502, 646, 709, 754, 803, 887, 911
NlaIV GGNNCC 1 cut(s) 816
NmeAIII GCCGAG 1 cut(s) 254
NmuCI GTSAC 4 cut(s) 110, 118, 248, 382
NspI RCATGY 3 cut(s) 87, 646, 754
PaeR7I CTCGAG 1 cut(s) 317
PciI ACATGT 2 cut(s) 642, 750
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 3 cut(s) 50, 573, 763
PflMI CCANNNNNTGG 1 cut(s) 541
PkrI GCNGC 3 cut(s) 154, 200, 862
PmaCI CACGTG 1 cut(s) 302
PmlI CACGTG 1 cut(s) 302
Ppu21I YACGTR 1 cut(s) 302
PpuMI RGGWCCY 1 cut(s) 814
PscI ACATGT 2 cut(s) 642, 750
Psp124BI GAGCTC 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 814
Psp6I CCWGG 1 cut(s) 855
PspCI CACGTG 1 cut(s) 302
PspGI CCWGG 1 cut(s) 855
PspN4I GGNNCC 1 cut(s) 816
PspPI GGNCC 3 cut(s) 398, 814, 853
PspPPI RGGWCCY 1 cut(s) 814
PspXI VCTCGAGB 1 cut(s) 317
PstI CTGCAG 1 cut(s) 244
PstNI CAGNNNCTG 2 cut(s) 638, 766
PsuI RGATCY 1 cut(s) 634
RsaI GTAC 2 cut(s) 647, 845
RsaNI GTAC 2 cut(s) 646, 844
RseI CAYNNNNRTG 2 cut(s) 704, 948
Rsr2I CGGWCCG 1 cut(s) 398
RsrII CGGWCCG 1 cut(s) 398
SacI GAGCTC 1 cut(s) 324
SacII CCGCGG 1 cut(s) 153
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 6 cut(s) 234, 810, 915, 960, 969, 976
SatI GCNGC 3 cut(s) 153, 199, 861
Sau3AI GATC 3 cut(s) 501, 634, 796
Sau96I GGNCC 3 cut(s) 398, 814, 853
ScrFI CCNGG 3 cut(s) 333, 735, 857
SduI GDGCHC 3 cut(s) 324, 562, 669
SfaNI GCATC 2 cut(s) 193, 943
SfcI CTRYAG 1 cut(s) 240
Sfr274I CTCGAG 1 cut(s) 317
Sfr303I CCGCGG 1 cut(s) 153
SgrBI CCGCGG 1 cut(s) 153
SinI GGWCC 3 cut(s) 398, 814, 853
SlaI CTCGAG 1 cut(s) 317
SmiMI CAYNNNNRTG 2 cut(s) 704, 948
SmlI CTYRAG 3 cut(s) 317, 660, 668
SmoI CTYRAG 3 cut(s) 317, 660, 668
SpeI ACTAGT 1 cut(s) 454
Sse9I AATT 6 cut(s) 506, 567, 590, 610, 675, 912
SsiI CCGC 8 cut(s) 150, 152, 166, 312, 373, 396, 415, 711
SspMI CTAG 2 cut(s) 455, 491
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 3 cut(s) 331, 733, 855
TaaI ACNGT 1 cut(s) 118
TaiI ACGT 3 cut(s) 234, 304, 928
TaqI TCGA 5 cut(s) 96, 318, 744, 848, 965
TasI AATT 6 cut(s) 506, 567, 590, 610, 675, 912
TatI WGTACW 1 cut(s) 843
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 3 cut(s) 50, 573, 763
Tru1I TTAA 6 cut(s) 234, 810, 915, 960, 969, 976
Tru9I TTAA 6 cut(s) 234, 810, 915, 960, 969, 976
TscAI CASTG 2 cut(s) 244, 587
TseFI GTSAC 4 cut(s) 110, 118, 248, 382
TseI GCWGC 2 cut(s) 198, 860
Tsp45I GTSAC 4 cut(s) 110, 118, 248, 382
TspGWI ACGGA 6 cut(s) 98, 390, 439, 447, 809, 866
TspRI CASTG 2 cut(s) 244, 587
Van91I CCANNNNNTGG 1 cut(s) 541
VneI GTGCAC 1 cut(s) 558
VpaK11BI GGWCC 3 cut(s) 398, 814, 853
XapI RAATTY 3 cut(s) 506, 590, 675
XceI RCATGY 3 cut(s) 87, 646, 754
XhoI CTCGAG 1 cut(s) 317
XspI CTAG 2 cut(s) 455, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.