AT5G50090

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012810
Physical Location & Seq
Forward (+)
20369657 .. 20371391
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50090.1

Sequence Viewer

Length: 480 bp
ATGGGAAACTGTCAAGCAGTGGATACAGCGAGAGTGGTGATACAACACCCAAATGGGAAAGAAGAAAAGTTAAGTTGTCCAGTTTCTGCAAGTTATGTGATGAAGATGAATCCTGGCCACTGTGTTTCTCTCCTCATCTCTACCACCGCTCTCTCGTCAGCATCTTCCGGTCATGGAGGGCCTCTTAGGCTCACGAGGATCAAGCTCCTTCGTCCCACGGATACGCTTGTTCTCGGCCACGTCTATAGACTCATTACCACCAAAGAGGTTATGAAGGGTTTAATGGCCAAAAAATGTTCGAAGTTGAAGAAAGAAAGTAAAGGCTCGGATGATAAGCTAGAGATGGTGAAAGCAATAAACTCTACTAAGCTTGACAATGAAGATCAGCTGCAAATGAAGAAACAGGAGAAAGAAAGGTCAAGAATCTCAAGATCATGGCAGCCATCATTACAAAGCATATCGGAAGGAGGATCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

159

Amino Acids

17.44

Weight (kDa)

9.7

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 155 1.1e-23 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012810)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50090 AT5G50090 AT5G50090
fragaria_vesca FvH4_5g16570
malus_domestica MD06G1178700.v1.1 MD14G1185100.v1.1
prunus_persica Prupe.5G177800_v2.0.a1
pyrus_communis pycom06g15880 pycom14g15400
rosa_chinensis RchiOBHm_Chr7g0179551
rosa_multiflora Rmu_sc0005329.1_g000004
rosa_roxburghii Rroxscaffold_3G00273720
rosa_rugosa Rorug06G0425500
rosa_samantha Rh7AG025300 Rh7BG024800 Rh7CG026100 Rh7DG026100
rosa_wichuraiana Rw7G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 149
AciI CCGC 1 cut(s) 147
AclWI GGATC 2 cut(s) 206, 478
AcoI YGGCCR 3 cut(s) 115, 235, 285
AgsI TTSAA 1 cut(s) 307
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 1 cut(s) 112
AluBI AGCT 5 cut(s) 205, 337, 370, 388, 477
AluI AGCT 5 cut(s) 205, 337, 370, 388, 477
AlwI GGATC 2 cut(s) 206, 478
AlwNI CAGNNNCTG 1 cut(s) 86
AoxI GGCC 4 cut(s) 115, 179, 235, 285
ApeKI GCWGC 2 cut(s) 388, 439
AspS9I GGNCC 1 cut(s) 179
AsuHPI GGTGA 2 cut(s) 49, 358
AsuII TTCGAA 1 cut(s) 299
BaeI ACNNNNGTAYC 2 cut(s) 213, 246
BalI TGGCCA 2 cut(s) 117, 287
BauI CACGAG 1 cut(s) 193
BbvI GCAGC 2 cut(s) 375, 451
BccI CCATC 2 cut(s) 337, 451
BciT130I CCWGG 1 cut(s) 114
BciVI GTATCC 2 cut(s) 16, 214
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 244
BfuI GTATCC 2 cut(s) 16, 214
BglI GCCNNNNNGGC 1 cut(s) 187
BisI GCNGC 2 cut(s) 389, 440
BlsI GCNGC 2 cut(s) 390, 441
Bme1390I CCNGG 1 cut(s) 114
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 1 cut(s) 179
BmrFI CCNGG 1 cut(s) 114
BmsI GCATC 1 cut(s) 170
Bpu14I TTCGAA 1 cut(s) 299
BpuEI CTTGAG 1 cut(s) 412
BsaJI CCNNGG 1 cut(s) 216
BsaWI WCCGGW 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 80
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 1 cut(s) 334
BseNI ACTGG 1 cut(s) 80
BseRI GAGGAG 1 cut(s) 122
BseXI GCAGC 2 cut(s) 375, 451
BshFI GGCC 4 cut(s) 117, 181, 237, 287
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 1 cut(s) 198
BsmFI GGGAC 1 cut(s) 198
BsnI GGCC 4 cut(s) 117, 181, 237, 287
Bsp119I TTCGAA 1 cut(s) 299
Bsp143I GATC 4 cut(s) 198, 382, 431, 470
BspACI CCGC 1 cut(s) 147
BspANI GGCC 4 cut(s) 117, 181, 237, 287
BspPI GGATC 2 cut(s) 206, 478
BspT104I TTCGAA 1 cut(s) 299
BsrBI CCGCTC 1 cut(s) 149
BsrI ACTGG 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 4 cut(s) 198, 382, 431, 470
BssSI CACGAG 1 cut(s) 193
Bst2BI CACGAG 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 2 cut(s) 11, 122
BstBI TTCGAA 1 cut(s) 299
BstDEI CTNAG 2 cut(s) 185, 366
BstDSI CCRYGG 1 cut(s) 216
BstF5I GGATG 1 cut(s) 334
BstKTI GATC 4 cut(s) 201, 385, 434, 473
BstMBI GATC 4 cut(s) 198, 382, 431, 470
BstMWI GCNNNNNNNGC 1 cut(s) 187
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 112
BstSFI CTRYAG 1 cut(s) 244
BstV1I GCAGC 2 cut(s) 375, 451
BsuI GTATCC 2 cut(s) 16, 214
BsuRI GGCC 4 cut(s) 117, 181, 237, 287
BtgI CCRYGG 1 cut(s) 216
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 1 cut(s) 334
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 2 cut(s) 24, 118
CaiI CAGNNNCTG 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 179
CviAII CATG 2 cut(s) 173, 435
DdeI CTNAG 2 cut(s) 185, 366
DpnI GATC 4 cut(s) 200, 384, 433, 472
DpnII GATC 4 cut(s) 198, 382, 431, 470
EaeI YGGCCR 3 cut(s) 115, 235, 285
EcoO109I RGGNCCY 1 cut(s) 179
EcoRII CCWGG 1 cut(s) 112
FaeI CATG 2 cut(s) 176, 438
FaiI YATR 6 cut(s) 96, 174, 246, 272, 436, 458
FaqI GGGAC 1 cut(s) 198
FatI CATG 2 cut(s) 172, 434
Fnu4HI GCNGC 2 cut(s) 389, 440
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 2 cut(s) 389, 440
FspBI CTAG 1 cut(s) 338
GluI GCNGC 2 cut(s) 389, 440
HaeIII GGCC 4 cut(s) 117, 181, 237, 287
HapII CCGG 1 cut(s) 168
Hin1II CATG 2 cut(s) 176, 438
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 3 cut(s) 109, 249, 423
HpaII CCGG 1 cut(s) 168
HphI GGTGA 2 cut(s) 49, 358
Hpy188I TCNGA 2 cut(s) 328, 463
Hpy188III TCNNGA 3 cut(s) 193, 420, 429
HpyAV CCTTC 3 cut(s) 218, 268, 458
HpyCH4III ACNGT 2 cut(s) 11, 122
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 2 cut(s) 89, 391
HpyF10VI GCNNNNNNNGC 1 cut(s) 187
HpyF3I CTNAG 2 cut(s) 185, 366
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 2 cut(s) 176, 438
Kzo9I GATC 4 cut(s) 198, 382, 431, 470
LmnI GCTCC 1 cut(s) 210
LpnPI CCDG 5 cut(s) 93, 99, 126, 181, 389
Lsp1109I GCAGC 2 cut(s) 375, 451
LweI GCATC 1 cut(s) 170
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 240
MalI GATC 4 cut(s) 200, 384, 433, 472
MbiI CCGCTC 1 cut(s) 149
MboI GATC 4 cut(s) 198, 382, 431, 470
MboII GAAGA 6 cut(s) 74, 115, 156, 319, 392, 409
MlsI TGGCCA 2 cut(s) 117, 287
MluNI TGGCCA 2 cut(s) 117, 287
MlyI GAGTC 1 cut(s) 243
MnlI CCTC 6 cut(s) 143, 170, 189, 192, 259, 461
Mox20I TGGCCA 2 cut(s) 117, 287
MscI TGGCCA 2 cut(s) 117, 287
MseI TTAA 2 cut(s) 71, 281
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 2 cut(s) 117, 287
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 187
NdeII GATC 4 cut(s) 198, 382, 431, 470
NlaIII CATG 2 cut(s) 176, 438
NmeAIII GCCGAG 1 cut(s) 213
NspV TTCGAA 1 cut(s) 299
PfeI GAWTC 2 cut(s) 109, 423
PkrI GCNGC 2 cut(s) 390, 441
PleI GAGTC 1 cut(s) 243
PpsI GAGTC 1 cut(s) 243
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspPI GGNCC 1 cut(s) 179
PstNI CAGNNNCTG 1 cut(s) 86
PvuII CAGCTG 1 cut(s) 388
RseI CAYNNNNRTG 1 cut(s) 51
SaqAI TTAA 2 cut(s) 71, 281
SatI GCNGC 2 cut(s) 389, 440
Sau3AI GATC 4 cut(s) 198, 382, 431, 470
Sau96I GGNCC 1 cut(s) 179
SchI GAGTC 1 cut(s) 243
ScrFI CCNGG 1 cut(s) 114
SetI ASST 8 cut(s) 207, 243, 270, 339, 372, 390, 419, 479
SfaNI GCATC 1 cut(s) 170
SfcI CTRYAG 1 cut(s) 244
SfuI TTCGAA 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
SsiI CCGC 1 cut(s) 147
SspMI CTAG 1 cut(s) 338
StyD4I CCNGG 1 cut(s) 112
TaaI ACNGT 2 cut(s) 11, 122
TaiI ACGT 1 cut(s) 243
TaqI TCGA 1 cut(s) 299
TfiI GAWTC 2 cut(s) 109, 423
Tru1I TTAA 2 cut(s) 71, 281
Tru9I TTAA 2 cut(s) 71, 281
TscAI CASTG 2 cut(s) 24, 125
TseI GCWGC 2 cut(s) 388, 439
TspDTI ATGAA 5 cut(s) 116, 122, 287, 393, 410
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 2 cut(s) 24, 125
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.