Rh7DG026100

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012810
Physical Location & Seq
Forward (+)
2007286 .. 2009477
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG026100.1

Sequence Viewer

Length: 567 bp
ATGGGAAATTGCCAAGCCATAGATGCAGCAACTTTGGTGATACAACACCCAAATGGGAAGGTCGAGAAGTTCTATGGGGCAGTGAGTGCTAGTGAGGTCATGAAGATGAACCCTGGTCACTATGTTGCTCTTCTCATCTCCACCACACTCTGTCCCTCATCAAATCCCAAATCCAAAACCAATGGTCCTGATAATAATGCTGGTGATGATCATGATCACAAGAATTCTGATATGACCAATGAGAAGAAGACTAGTTCTAGTACTGGTTCAGTTCGAATCACACGCATCAAGCTTCTCAGGGCAACTGATACTCTTGTTCTTGGCCAAGTTTACAGACTCATCACCACTCAAGAGGTTATGAAGGGTCTGTGGGCAAAGAAACAAGCAAAGGGGAAGAGATCAAACCAGTTTGAGCCACTAACAGCGGAGAGGTTGCAGAGAGAGACAGTAGCAGCAGCAGCTAGCAGAAGATCTGAGGCTGCTTCTGAGCTGGACAAGGACAACAACCAGGTAATTAAAATTTTGTTTAGCACAATTATGTCTCTGCAAATTCTGCATTTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

188

Amino Acids

20.64

Weight (kDa)

9.49

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 163 1.1e-19 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012810)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50090 AT5G50090 AT5G50090
fragaria_vesca FvH4_5g16570
malus_domestica MD06G1178700.v1.1 MD14G1185100.v1.1
prunus_persica Prupe.5G177800_v2.0.a1
pyrus_communis pycom06g15880 pycom14g15400
rosa_chinensis RchiOBHm_Chr7g0179551
rosa_multiflora Rmu_sc0005329.1_g000004
rosa_roxburghii Rroxscaffold_3G00273720
rosa_rugosa Rorug06G0425500
rosa_samantha Rh7AG025300 Rh7BG024800 Rh7CG026100 Rh7DG026100
rosa_wichuraiana Rw7G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 425
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 3 cut(s) 223, 519, 549
AfaI GTAC 1 cut(s) 262
AhlI ACTAGT 1 cut(s) 251
AjnI CCWGG 2 cut(s) 112, 507
AluBI AGCT 3 cut(s) 292, 461, 490
AluI AGCT 3 cut(s) 292, 461, 490
Alw26I GTCTC 2 cut(s) 437, 546
AoxI GGCC 1 cut(s) 322
ApeKI GCWGC 5 cut(s) 26, 452, 455, 458, 479
ApoI RAATTY 3 cut(s) 223, 519, 549
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 3 cut(s) 49, 215, 334
AsuII TTCGAA 1 cut(s) 274
AsuNHI GCTAGC 1 cut(s) 461
AvaII GGWCC 1 cut(s) 185
BaeI ACNNNNGTAYC 2 cut(s) 300, 333
BalI TGGCCA 1 cut(s) 324
BbsI GAAGAC 1 cut(s) 254
BbvI GCAGC 5 cut(s) 38, 464, 466, 467, 470
BciT130I CCWGG 2 cut(s) 114, 509
BclI TGATCA 2 cut(s) 208, 214
BcoDI GTCTC 2 cut(s) 437, 546
BcuI ACTAGT 1 cut(s) 251
BfaI CTAG 4 cut(s) 90, 252, 258, 462
BglII AGATCT 1 cut(s) 470
BisI GCNGC 5 cut(s) 27, 453, 456, 459, 480
BlsI GCNGC 5 cut(s) 28, 454, 457, 460, 481
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 114, 509
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmrFI CCNGG 2 cut(s) 114, 509
BmsI GCATC 2 cut(s) 13, 294
BmtI GCTAGC 1 cut(s) 465
BpiI GAAGAC 1 cut(s) 254
Bpu14I TTCGAA 1 cut(s) 274
BpuEI CTTGAG 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 112
Bse1I ACTGG 2 cut(s) 268, 406
Bse8I GATNNNNATC 1 cut(s) 213
BseBI CCWGG 2 cut(s) 114, 509
BseDI CCNNGG 1 cut(s) 112
BseJI GATNNNNATC 1 cut(s) 213
BseMII CTCAG 3 cut(s) 310, 465, 477
BseNI ACTGG 2 cut(s) 268, 406
BseXI GCAGC 5 cut(s) 38, 464, 466, 467, 470
BshFI GGCC 1 cut(s) 324
BslFI GGGAC 1 cut(s) 138
BsmAI GTCTC 2 cut(s) 437, 546
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 1 cut(s) 324
Bsp119I TTCGAA 1 cut(s) 274
Bsp143I GATC 4 cut(s) 208, 214, 398, 470
BspACI CCGC 1 cut(s) 425
BspANI GGCC 1 cut(s) 324
BspCNI CTCAG 3 cut(s) 309, 466, 478
BspHI TCATGA 2 cut(s) 99, 211
BspOI GCTAGC 1 cut(s) 465
BspQI GCTCTTC 1 cut(s) 135
BspT104I TTCGAA 1 cut(s) 274
BsrI ACTGG 2 cut(s) 268, 406
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 4 cut(s) 208, 214, 398, 470
Bst2UI CCWGG 2 cut(s) 114, 509
Bst4CI ACNGT 1 cut(s) 448
Bst6I CTCTTC 2 cut(s) 135, 389
BstAPI GCANNNNNTGC 2 cut(s) 86, 553
BstBI TTCGAA 1 cut(s) 274
BstC8I GCNNGC 1 cut(s) 463
BstDEI CTNAG 3 cut(s) 296, 474, 486
BstKTI GATC 4 cut(s) 211, 217, 401, 473
BstMAI GTCTC 2 cut(s) 437, 546
BstMBI GATC 4 cut(s) 208, 214, 398, 470
BstMWI GCNNNNNNNGC 4 cut(s) 23, 86, 458, 553
BstNI CCWGG 2 cut(s) 114, 509
BstSCI CCNGG 2 cut(s) 112, 507
BstV1I GCAGC 5 cut(s) 38, 464, 466, 467, 470
BstV2I GAAGAC 1 cut(s) 254
BstX2I RGATCY 1 cut(s) 470
BstYI RGATCY 1 cut(s) 470
BsuRI GGCC 1 cut(s) 324
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 463
CciI TCATGA 2 cut(s) 99, 211
Cfr13I GGNCC 1 cut(s) 185
CsiI ACCWGGT 1 cut(s) 507
Csp6I GTAC 1 cut(s) 261
CviAII CATG 2 cut(s) 100, 212
CviJI RGCY 7 cut(s) 17, 292, 324, 415, 461, 479, 490
CviKI_1 RGCY 7 cut(s) 17, 292, 324, 415, 461, 479, 490
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 3 cut(s) 296, 474, 486
DpnI GATC 4 cut(s) 210, 216, 400, 472
DpnII GATC 4 cut(s) 208, 214, 398, 470
EaeI YGGCCR 1 cut(s) 322
Eam1104I CTCTTC 2 cut(s) 135, 389
EarI CTCTTC 2 cut(s) 135, 389
Eco47I GGWCC 1 cut(s) 185
EcoRI GAATTC 1 cut(s) 223
EcoRII CCWGG 2 cut(s) 112, 507
FaeI CATG 2 cut(s) 103, 215
FaiI YATR 8 cut(s) 20, 75, 101, 123, 213, 233, 359, 539
FaqI GGGAC 1 cut(s) 138
FatI CATG 2 cut(s) 99, 211
FbaI TGATCA 2 cut(s) 208, 214
Fnu4HI GCNGC 5 cut(s) 27, 453, 456, 459, 480
Fsp4HI GCNGC 5 cut(s) 27, 453, 456, 459, 480
FspBI CTAG 4 cut(s) 90, 252, 258, 462
GluI GCNGC 5 cut(s) 27, 453, 456, 459, 480
HaeIII GGCC 1 cut(s) 324
Hin1II CATG 2 cut(s) 103, 215
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 2 cut(s) 276, 336
HphI GGTGA 3 cut(s) 49, 215, 334
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 3 cut(s) 229, 475, 487
Hpy188III TCNNGA 5 cut(s) 64, 100, 188, 212, 350
Hpy8I GTNNAC 1 cut(s) 331
HpyAV CCTTC 2 cut(s) 52, 355
HpyCH4III ACNGT 1 cut(s) 448
HpyCH4V TGCA 4 cut(s) 26, 436, 547, 556
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 86, 458, 553
HpyF3I CTNAG 3 cut(s) 296, 474, 486
Hsp92II CATG 2 cut(s) 103, 215
Ksp22I TGATCA 2 cut(s) 208, 214
Kzo9I GATC 4 cut(s) 208, 214, 398, 470
LguI GCTCTTC 1 cut(s) 135
Lsp1109I GCAGC 5 cut(s) 38, 464, 466, 467, 470
LweI GCATC 2 cut(s) 13, 294
MabI ACCWGGT 1 cut(s) 507
MaeI CTAG 4 cut(s) 90, 252, 258, 462
MaeIII GTNAC 1 cut(s) 116
MalI GATC 4 cut(s) 210, 216, 400, 472
MboI GATC 4 cut(s) 208, 214, 398, 470
MboII GAAGA 6 cut(s) 115, 122, 256, 259, 406, 480
MflI RGATCY 1 cut(s) 470
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 6 cut(s) 7, 223, 513, 519, 534, 549
MluNI TGGCCA 1 cut(s) 324
MlyI GAGTC 1 cut(s) 330
MnlI CCTC 5 cut(s) 88, 166, 346, 423, 469
Mox20I TGGCCA 1 cut(s) 324
MscI TGGCCA 1 cut(s) 324
MseI TTAA 1 cut(s) 516
MslI CAYNNNNRTG 3 cut(s) 51, 104, 536
Msp20I TGGCCA 1 cut(s) 324
MspA1I CMGCKG 1 cut(s) 425
MspR9I CCNGG 2 cut(s) 114, 509
MvaI CCWGG 2 cut(s) 114, 509
MwoI GCNNNNNNNGC 4 cut(s) 23, 86, 458, 553
NdeII GATC 4 cut(s) 208, 214, 398, 470
NheI GCTAGC 1 cut(s) 461
NlaIII CATG 2 cut(s) 103, 215
NmuCI GTSAC 1 cut(s) 116
NspV TTCGAA 1 cut(s) 274
PagI TCATGA 2 cut(s) 99, 211
PciSI GCTCTTC 1 cut(s) 135
PfeI GAWTC 1 cut(s) 276
PkrI GCNGC 5 cut(s) 28, 454, 457, 460, 481
PleI GAGTC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 330
Psp6I CCWGG 2 cut(s) 112, 507
PspGI CCWGG 2 cut(s) 112, 507
PspPI GGNCC 1 cut(s) 185
PsuI RGATCY 1 cut(s) 470
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
RseI CAYNNNNRTG 3 cut(s) 51, 104, 536
SapI GCTCTTC 1 cut(s) 135
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 5 cut(s) 27, 453, 456, 459, 480
Sau3AI GATC 4 cut(s) 208, 214, 398, 470
Sau96I GGNCC 1 cut(s) 185
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 1 cut(s) 330
ScrFI CCNGG 2 cut(s) 114, 509
SetI ASST 8 cut(s) 63, 99, 294, 357, 434, 463, 492, 513
SexAI ACCWGGT 1 cut(s) 507
SfaNI GCATC 2 cut(s) 13, 294
SfuI TTCGAA 1 cut(s) 274
SinI GGWCC 1 cut(s) 185
SmiMI CAYNNNNRTG 3 cut(s) 51, 104, 536
SmlI CTYRAG 1 cut(s) 348
SmoI CTYRAG 1 cut(s) 348
SpeI ACTAGT 1 cut(s) 251
Sse9I AATT 6 cut(s) 7, 223, 513, 519, 534, 549
SsiI CCGC 1 cut(s) 425
SspMI CTAG 4 cut(s) 90, 252, 258, 462
StyD4I CCNGG 2 cut(s) 112, 507
TaaI ACNGT 1 cut(s) 448
TaqI TCGA 2 cut(s) 63, 274
TasI AATT 6 cut(s) 7, 223, 513, 519, 534, 549
TatI WGTACW 1 cut(s) 260
TfiI GAWTC 1 cut(s) 276
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 1 cut(s) 87
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 5 cut(s) 26, 452, 455, 458, 479
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 116, 122, 374
TspRI CASTG 1 cut(s) 87
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 3 cut(s) 223, 519, 549
XspI CTAG 4 cut(s) 90, 252, 258, 462
ZrmI AGTACT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.