MD14G1185100.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012810
Physical Location & Seq
Forward (+)
27751148 .. 27752958
1811 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1185100.v1.1.491

Sequence Viewer

Length: 585 bp
ATGGGAAATTGTCAAGCGACTGATGCAGCAACACTGGTAGTACAGCACCCAAGTGGGAAAGAAGAGAAGTTTTATTGGGCAGTGAGTGCAAGTGAGATTATGAAGATGAACCCTGGCCACTATGTTGCTCTTCTCATCTCCACCACCTTGTGTCCCTCCAATCCCACCACGGACAAGGGCGGTGACAGGAAAGACAAGAACTCGAATCCGGACGACAACAAGAACACCGCTTCTTCGCTTCGCGTGACGCGCATAAAGCTCCTCAGGCCAACCGATTCTCTTGTTCTTGGCAAAGTTTACAGACTCATCACCACTCAAGTGATGAAGGGATTGTGGGCAAAGAAACAAGCGAAGGTGAAGAGAAATAATAATCATCAGGTGGAAGGAGAGCAGAAGCCACAAGTGGAGAGGGTGATGAGAGAGAAGCAAGTTCTGACATCAGCCAGAAGATCTGAGACTTCTGAGCTGGAGAAGGACATCCAGGTGAATAATAAACATGAGAGAGGAGGACACCGACCAAGAACAGCAACAACATCAAGAACGTGGCAGCCATCATTACACAGCATCTCAGAGGCTGCAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

195

Amino Acids

21.73

Weight (kDa)

9.89

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 191 5.4e-25 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012810)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50090 AT5G50090 AT5G50090
fragaria_vesca FvH4_5g16570
malus_domestica MD06G1178700.v1.1 MD14G1185100.v1.1
prunus_persica Prupe.5G177800_v2.0.a1
pyrus_communis pycom06g15880 pycom14g15400
rosa_chinensis RchiOBHm_Chr7g0179551
rosa_multiflora Rmu_sc0005329.1_g000004
rosa_roxburghii Rroxscaffold_3G00273720
rosa_rugosa Rorug06G0425500
rosa_samantha Rh7AG025300 Rh7BG024800 Rh7CG026100 Rh7DG026100
rosa_wichuraiana Rw7G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 243, 250
AccIII TCCGGA 1 cut(s) 208
AciI CCGC 2 cut(s) 180, 228
AcoI YGGCCR 1 cut(s) 115
AdeI CACNNNGTG 1 cut(s) 150
AfaI GTAC 1 cut(s) 42
AjnI CCWGG 2 cut(s) 112, 480
AleI CACNNNNGTG 2 cut(s) 51, 317
AluBI AGCT 2 cut(s) 259, 466
AluI AGCT 2 cut(s) 259, 466
Alw26I GTCTC 1 cut(s) 449
AlwNI CAGNNNCTG 1 cut(s) 575
Aor13HI TCCGGA 1 cut(s) 208
AoxI GGCC 2 cut(s) 115, 266
ApeKI GCWGC 3 cut(s) 26, 547, 575
AspLEI GCGC 1 cut(s) 252
AsuHPI GGTGA 5 cut(s) 194, 301, 367, 424, 496
AxyI CCTNAGG 1 cut(s) 263
BalI TGGCCA 1 cut(s) 117
BbvI GCAGC 3 cut(s) 38, 559, 562
BccI CCATC 1 cut(s) 559
BciT130I CCWGG 2 cut(s) 114, 482
BcoDI GTCTC 1 cut(s) 449
BglII AGATCT 1 cut(s) 449
BisI GCNGC 3 cut(s) 27, 548, 576
BlsI GCNGC 3 cut(s) 28, 549, 577
Bme1390I CCNGG 2 cut(s) 114, 482
BmrFI CCNGG 2 cut(s) 114, 482
BmsI GCATC 2 cut(s) 13, 573
BpmI CTGGAG 1 cut(s) 488
BpuEI CTTGAG 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 112, 168
BsaWI WCCGGW 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 39
Bse21I CCTNAGG 1 cut(s) 263
BseAI TCCGGA 1 cut(s) 208
BseBI CCWGG 2 cut(s) 114, 482
BseDI CCNNGG 2 cut(s) 112, 168
BseGI GGATG 1 cut(s) 477
BseMII CTCAG 4 cut(s) 277, 444, 453, 582
BseNI ACTGG 1 cut(s) 39
BseRI GAGGAG 2 cut(s) 251, 519
BseXI GCAGC 3 cut(s) 38, 559, 562
Bsh1236I CGCG 2 cut(s) 243, 250
BshFI GGCC 2 cut(s) 117, 268
BsiSI CCGG 1 cut(s) 209
BslFI GGGAC 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 449
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 2 cut(s) 117, 268
Bsp13I TCCGGA 1 cut(s) 208
Bsp143I GATC 1 cut(s) 449
BspACI CCGC 2 cut(s) 180, 228
BspANI GGCC 2 cut(s) 117, 268
BspCNI CTCAG 4 cut(s) 276, 445, 454, 581
BspEI TCCGGA 1 cut(s) 208
BspFNI CGCG 2 cut(s) 243, 250
BspQI GCTCTTC 1 cut(s) 135
BsrI ACTGG 1 cut(s) 39
BssECI CCNNGG 2 cut(s) 112, 168
BssMI GATC 1 cut(s) 449
Bst2UI CCWGG 2 cut(s) 114, 482
Bst6I CTCTTC 3 cut(s) 57, 135, 353
BstAPI GCANNNNNTGC 1 cut(s) 86
BstDEI CTNAG 4 cut(s) 263, 453, 462, 568
BstDSI CCRYGG 1 cut(s) 168
BstF5I GGATG 1 cut(s) 477
BstFNI CGCG 2 cut(s) 243, 250
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 1 cut(s) 452
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 1 cut(s) 449
BstMWI GCNNNNNNNGC 5 cut(s) 23, 86, 249, 256, 265
BstNI CCWGG 2 cut(s) 114, 482
BstSCI CCNGG 2 cut(s) 112, 480
BstUI CGCG 2 cut(s) 243, 250
BstV1I GCAGC 3 cut(s) 38, 559, 562
BstX2I RGATCY 1 cut(s) 449
BstYI RGATCY 1 cut(s) 449
Bsu36I CCTNAGG 1 cut(s) 263
BsuRI GGCC 2 cut(s) 117, 268
BtgI CCRYGG 1 cut(s) 168
BtsCI GGATG 1 cut(s) 477
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 2 cut(s) 32, 87
CaiI CAGNNNCTG 1 cut(s) 575
CfoI GCGC 1 cut(s) 252
CseI GACGC 1 cut(s) 256
Csp6I GTAC 1 cut(s) 41
CviAII CATG 1 cut(s) 497
CviJI RGCY 8 cut(s) 117, 259, 268, 397, 443, 466, 550, 575
CviKI_1 RGCY 8 cut(s) 117, 259, 268, 397, 443, 466, 550, 575
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 4 cut(s) 263, 453, 462, 568
DpnI GATC 1 cut(s) 451
DpnII GATC 1 cut(s) 449
DraIII CACNNNGTG 1 cut(s) 150
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 3 cut(s) 57, 135, 353
EarI CTCTTC 3 cut(s) 57, 135, 353
Eco81I CCTNAGG 1 cut(s) 263
EcoRII CCWGG 2 cut(s) 112, 480
FaeI CATG 1 cut(s) 500
FaiI YATR 4 cut(s) 101, 123, 254, 498
FaqI GGGAC 1 cut(s) 138
FatI CATG 1 cut(s) 496
Fnu4HI GCNGC 3 cut(s) 27, 548, 576
FokI GGATG 1 cut(s) 464
Fsp4HI GCNGC 3 cut(s) 27, 548, 576
GlaI GCGC 1 cut(s) 251
GluI GCNGC 3 cut(s) 27, 548, 576
GsuI CTGGAG 1 cut(s) 488
HaeIII GGCC 2 cut(s) 117, 268
HapII CCGG 1 cut(s) 209
HgaI GACGC 1 cut(s) 256
HhaI GCGC 1 cut(s) 252
Hin1II CATG 1 cut(s) 500
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HinfI GANTC 3 cut(s) 205, 275, 303
HpaII CCGG 1 cut(s) 209
HphI GGTGA 5 cut(s) 194, 301, 367, 424, 496
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 4 cut(s) 435, 454, 463, 571
Hpy188III TCNNGA 2 cut(s) 209, 537
Hpy8I GTNNAC 1 cut(s) 298
HpyAV CCTTC 4 cut(s) 319, 346, 377, 466
HpyCH4IV ACGT 1 cut(s) 542
HpyCH4V TGCA 3 cut(s) 26, 89, 578
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 86, 249, 256, 265
HpyF3I CTNAG 4 cut(s) 263, 453, 462, 568
HpySE526I ACGT 1 cut(s) 542
Hsp92II CATG 1 cut(s) 500
HspAI GCGC 1 cut(s) 250
Kpn2I TCCGGA 1 cut(s) 208
Kzo9I GATC 1 cut(s) 449
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 264
Lsp1109I GCAGC 3 cut(s) 38, 559, 562
LweI GCATC 2 cut(s) 13, 573
MaeII ACGT 1 cut(s) 542
MaeIII GTNAC 2 cut(s) 182, 244
MalI GATC 1 cut(s) 451
MboI GATC 1 cut(s) 449
MboII GAAGA 6 cut(s) 74, 115, 122, 225, 370, 459
MflI RGATCY 1 cut(s) 449
MlsI TGGCCA 1 cut(s) 117
MluCI AATT 1 cut(s) 7
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 1 cut(s) 297
MnlI CCTC 6 cut(s) 166, 272, 402, 497, 500, 565
Mox20I TGGCCA 1 cut(s) 117
MroI TCCGGA 1 cut(s) 208
MscI TGGCCA 1 cut(s) 117
MslI CAYNNNNRTG 3 cut(s) 51, 317, 482
Msp20I TGGCCA 1 cut(s) 117
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 2 cut(s) 114, 482
MvaI CCWGG 2 cut(s) 114, 482
MvnI CGCG 2 cut(s) 243, 250
MwoI GCNNNNNNNGC 5 cut(s) 23, 86, 249, 256, 265
NdeII GATC 1 cut(s) 449
NlaIII CATG 1 cut(s) 500
NmuCI GTSAC 2 cut(s) 182, 244
OliI CACNNNNGTG 2 cut(s) 51, 317
PciSI GCTCTTC 1 cut(s) 135
PfeI GAWTC 2 cut(s) 205, 275
PkrI GCNGC 3 cut(s) 28, 549, 577
PleI GAGTC 1 cut(s) 297
PpsI GAGTC 1 cut(s) 297
Psp6I CCWGG 2 cut(s) 112, 480
PspGI CCWGG 2 cut(s) 112, 480
PstNI CAGNNNCTG 1 cut(s) 575
PsuI RGATCY 1 cut(s) 449
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
RseI CAYNNNNRTG 3 cut(s) 51, 317, 482
SapI GCTCTTC 1 cut(s) 135
SatI GCNGC 3 cut(s) 27, 548, 576
Sau3AI GATC 1 cut(s) 449
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 2 cut(s) 114, 482
SetI ASST 7 cut(s) 149, 261, 357, 381, 468, 486, 545
SfaNI GCATC 2 cut(s) 13, 573
SmiMI CAYNNNNRTG 3 cut(s) 51, 317, 482
SmlI CTYRAG 1 cut(s) 315
SmoI CTYRAG 1 cut(s) 315
Sse9I AATT 1 cut(s) 7
SsiI CCGC 2 cut(s) 180, 228
StyD4I CCNGG 2 cut(s) 112, 480
TaiI ACGT 1 cut(s) 545
TaqI TCGA 1 cut(s) 203
TasI AATT 1 cut(s) 7
TatI WGTACW 1 cut(s) 40
TfiI GAWTC 2 cut(s) 205, 275
TscAI CASTG 2 cut(s) 39, 87
TseFI GTSAC 2 cut(s) 182, 244
TseI GCWGC 3 cut(s) 26, 547, 575
Tsp45I GTSAC 2 cut(s) 182, 244
TspDTI ATGAA 3 cut(s) 116, 122, 338
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 2 cut(s) 39, 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.