MD06G1178700.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012810
Physical Location & Seq
Forward (+)
31875929 .. 31877987
2059 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1178700.v1.1.491

Sequence Viewer

Length: 750 bp
ATGTTGGTGGGTGGCTATAAAGAGCTTGTTGTAGAGCTCAAACCACGTCCTCTTTCCCCTATCTTCTCATCCCTTTTCTTCAACTTCACTCTAGAGAGAGAGAGAGAGAGAGAGAGAGGAGAGAGAGAGGAACTAGAGGGAGAAATGGGAAATTGTCAGGCAATTGATGCAGCAACACTGGTAGTACAACACCCAAGTGGGAAAGAAGAGAAGTTCTATGGGGAAGTGAGTGCAAGTGAGATCATGAAGATGAACCCTGGCCACTATGTTGCTCTTCTCATCTCCACCACCTTGTGTCCCTCCAATCCCACCACCGACACTGGCGGTGACCGGAAATACAAGAATTCGAGTCCAAACGACAACAAGAATACCGCTTCTTCGGTTCGAGTGACGCGCATAAAGCTCCTCAGGCCAACCGAATCTCTTGTTCTTGGCAAAGTTTACAGACTCATCGCCACTCAAGAAGTGATGAAGGGATTGTGGGCAAAGAAACAAGCGAAGGTGAAGAGAAATAACAATCATCGGGTGGAAGGAGAGCAGCAGCCACAAGTAGAGAGGGTGATGAGAGAGAAGCAAGTTCTGACATCAGGCAGAAGATCTGAGACTTCTGAGCTGCTGGAGAAGGACATCCAGGTGAATAACAAACGTGAGAGAGCCCACCGACCAAGAACAACAACAACATCAAGAACGTGGCAGCCCTCATTACACAGCATCTCAGAGGCTGCAAGTCGAGATATCAATGTCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

250

Amino Acids

28.28

Weight (kDa)

9.58

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 49 - 240 3e-23 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012810)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50090 AT5G50090 AT5G50090
fragaria_vesca FvH4_5g16570
malus_domestica MD06G1178700.v1.1 MD14G1185100.v1.1
prunus_persica Prupe.5G177800_v2.0.a1
pyrus_communis pycom06g15880 pycom14g15400
rosa_chinensis RchiOBHm_Chr7g0179551
rosa_multiflora Rmu_sc0005329.1_g000004
rosa_roxburghii Rroxscaffold_3G00273720
rosa_rugosa Rorug06G0425500
rosa_samantha Rh7AG025300 Rh7BG024800 Rh7CG026100 Rh7DG026100
rosa_wichuraiana Rw7G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 394
AciI CCGC 2 cut(s) 324, 372
AcoI YGGCCR 1 cut(s) 259
AcsI RAATTY 1 cut(s) 343
AdeI CACNNNGTG 1 cut(s) 294
AfaI GTAC 1 cut(s) 186
AgsI TTSAA 1 cut(s) 82
AjiI CACGTC 1 cut(s) 47
AjnI CCWGG 2 cut(s) 256, 630
AleI CACNNNNGTG 1 cut(s) 195
AluBI AGCT 4 cut(s) 25, 37, 403, 613
AluI AGCT 4 cut(s) 25, 37, 403, 613
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 596
AlwNI CAGNNNCTG 1 cut(s) 722
AoxI GGCC 2 cut(s) 259, 410
ApeKI GCWGC 6 cut(s) 170, 538, 541, 613, 694, 722
ApoI RAATTY 1 cut(s) 343
AspLEI GCGC 1 cut(s) 396
AsuHPI GGTGA 4 cut(s) 338, 514, 571, 646
AxyI CCTNAGG 1 cut(s) 407
BalI TGGCCA 1 cut(s) 261
BanII GRGCYC 2 cut(s) 39, 658
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 6 cut(s) 182, 550, 553, 600, 706, 709
BciT130I CCWGG 2 cut(s) 258, 632
BcoDI GTCTC 1 cut(s) 596
BfaI CTAG 2 cut(s) 92, 134
BglII AGATCT 1 cut(s) 596
BisI GCNGC 6 cut(s) 171, 539, 542, 614, 695, 723
BlsI GCNGC 6 cut(s) 172, 540, 543, 615, 696, 724
Bme1390I CCNGG 2 cut(s) 258, 632
BmgBI CACGTC 1 cut(s) 47
BmrFI CCNGG 2 cut(s) 258, 632
BmsI GCATC 2 cut(s) 157, 720
BpmI CTGGAG 1 cut(s) 638
BpuEI CTTGAG 1 cut(s) 444
BsaJI CCNNGG 1 cut(s) 256
BsaWI WCCGGW 1 cut(s) 330
Bse1I ACTGG 2 cut(s) 183, 325
Bse21I CCTNAGG 1 cut(s) 407
BseBI CCWGG 2 cut(s) 258, 632
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 2 cut(s) 68, 627
BseMII CTCAG 4 cut(s) 421, 591, 600, 729
BseNI ACTGG 2 cut(s) 183, 325
BseRI GAGGAG 2 cut(s) 132, 395
BseXI GCAGC 6 cut(s) 182, 550, 553, 600, 706, 709
Bsh1236I CGCG 1 cut(s) 394
BshFI GGCC 2 cut(s) 261, 412
BsiHKAI GWGCWC 1 cut(s) 39
BsiSI CCGG 1 cut(s) 331
BslFI GGGAC 1 cut(s) 282
BsmAI GTCTC 1 cut(s) 596
BsmFI GGGAC 1 cut(s) 282
BsnI GGCC 2 cut(s) 261, 412
Bsp1286I GDGCHC 2 cut(s) 39, 658
Bsp143I GATC 2 cut(s) 240, 596
BspACI CCGC 2 cut(s) 324, 372
BspANI GGCC 2 cut(s) 261, 412
BspCNI CTCAG 4 cut(s) 420, 592, 601, 728
BspFNI CGCG 1 cut(s) 394
BspHI TCATGA 1 cut(s) 243
BspQI GCTCTTC 1 cut(s) 279
BsrI ACTGG 2 cut(s) 183, 325
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 2 cut(s) 240, 596
Bst2UI CCWGG 2 cut(s) 258, 632
Bst6I CTCTTC 3 cut(s) 201, 279, 500
BstAPI GCANNNNNTGC 1 cut(s) 167
BstDEI CTNAG 4 cut(s) 407, 600, 609, 715
BstEII GGTNACC 1 cut(s) 326
BstF5I GGATG 2 cut(s) 68, 627
BstFNI CGCG 1 cut(s) 394
BstHHI GCGC 1 cut(s) 396
BstKTI GATC 2 cut(s) 243, 599
BstMAI GTCTC 1 cut(s) 596
BstMBI GATC 2 cut(s) 240, 596
BstMWI GCNNNNNNNGC 3 cut(s) 167, 400, 409
BstNI CCWGG 2 cut(s) 258, 632
BstPI GGTNACC 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 256, 630
BstUI CGCG 1 cut(s) 394
BstV1I GCAGC 6 cut(s) 182, 550, 553, 600, 706, 709
BstX2I RGATCY 1 cut(s) 596
BstYI RGATCY 1 cut(s) 596
Bsu36I CCTNAGG 1 cut(s) 407
BsuRI GGCC 2 cut(s) 261, 412
BtgZI GCGATG 1 cut(s) 436
BtrI CACGTC 1 cut(s) 47
BtsCI GGATG 2 cut(s) 68, 627
BtsIMutI CAGTG 2 cut(s) 176, 318
CaiI CAGNNNCTG 1 cut(s) 722
CciI TCATGA 1 cut(s) 243
CfoI GCGC 1 cut(s) 396
CseI GACGC 1 cut(s) 400
Csp6I GTAC 1 cut(s) 185
CviAII CATG 1 cut(s) 244
CviQI GTAC 1 cut(s) 185
DdeI CTNAG 4 cut(s) 407, 600, 609, 715
DpnI GATC 2 cut(s) 242, 598
DpnII GATC 2 cut(s) 240, 596
DraIII CACNNNGTG 1 cut(s) 294
EaeI YGGCCR 1 cut(s) 259
Eam1104I CTCTTC 3 cut(s) 201, 279, 500
EarI CTCTTC 3 cut(s) 201, 279, 500
Ecl136II GAGCTC 1 cut(s) 37
Eco24I GRGCYC 2 cut(s) 39, 658
Eco32I GATATC 1 cut(s) 736
Eco53kI GAGCTC 1 cut(s) 37
Eco81I CCTNAGG 1 cut(s) 407
Eco91I GGTNACC 1 cut(s) 326
EcoICRI GAGCTC 1 cut(s) 37
EcoO65I GGTNACC 1 cut(s) 326
EcoRI GAATTC 1 cut(s) 343
EcoRII CCWGG 2 cut(s) 256, 630
EcoRV GATATC 1 cut(s) 736
EcoT38I GRGCYC 2 cut(s) 39, 658
FaeI CATG 1 cut(s) 247
FaiI YATR 5 cut(s) 18, 219, 245, 267, 398
FaqI GGGAC 1 cut(s) 282
FatI CATG 1 cut(s) 243
Fnu4HI GCNGC 6 cut(s) 171, 539, 542, 614, 695, 723
FokI GGATG 2 cut(s) 55, 614
FriOI GRGCYC 2 cut(s) 39, 658
Fsp4HI GCNGC 6 cut(s) 171, 539, 542, 614, 695, 723
FspBI CTAG 2 cut(s) 92, 134
GlaI GCGC 1 cut(s) 395
GluI GCNGC 6 cut(s) 171, 539, 542, 614, 695, 723
GsuI CTGGAG 1 cut(s) 638
HaeIII GGCC 2 cut(s) 261, 412
HapII CCGG 1 cut(s) 331
HgaI GACGC 1 cut(s) 400
HhaI GCGC 1 cut(s) 396
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HinfI GANTC 3 cut(s) 349, 419, 447
HpaII CCGG 1 cut(s) 331
HphI GGTGA 4 cut(s) 338, 514, 571, 646
Hpy166II GTNNAC 1 cut(s) 442
Hpy188I TCNGA 4 cut(s) 582, 601, 610, 718
Hpy188III TCNNGA 5 cut(s) 92, 244, 461, 684, 731
Hpy8I GTNNAC 1 cut(s) 442
HpyAV CCTTC 4 cut(s) 466, 493, 524, 616
HpyCH4IV ACGT 3 cut(s) 46, 646, 689
HpyCH4V TGCA 3 cut(s) 170, 233, 725
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 400, 409
HpyF3I CTNAG 4 cut(s) 407, 600, 609, 715
HpySE526I ACGT 3 cut(s) 46, 646, 689
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 1 cut(s) 394
Kzo9I GATC 2 cut(s) 240, 596
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 1 cut(s) 408
Lsp1109I GCAGC 6 cut(s) 182, 550, 553, 600, 706, 709
LweI GCATC 2 cut(s) 157, 720
MaeI CTAG 2 cut(s) 92, 134
MaeII ACGT 3 cut(s) 46, 646, 689
MaeIII GTNAC 2 cut(s) 326, 388
MalI GATC 2 cut(s) 242, 598
MboI GATC 2 cut(s) 240, 596
MboII GAAGA 8 cut(s) 55, 70, 218, 259, 266, 369, 517, 606
MfeI CAATTG 1 cut(s) 162
MflI RGATCY 1 cut(s) 596
MhlI GDGCHC 2 cut(s) 39, 658
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 3 cut(s) 151, 162, 343
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 2 cut(s) 358, 441
MnlI CCTC 9 cut(s) 60, 110, 121, 130, 310, 416, 549, 709, 712
Mox20I TGGCCA 1 cut(s) 261
MscI TGGCCA 1 cut(s) 261
MslI CAYNNNNRTG 3 cut(s) 195, 248, 632
Msp20I TGGCCA 1 cut(s) 261
MspI CCGG 1 cut(s) 331
MspR9I CCNGG 2 cut(s) 258, 632
MunI CAATTG 1 cut(s) 162
MvaI CCWGG 2 cut(s) 258, 632
MvnI CGCG 1 cut(s) 394
MwoI GCNNNNNNNGC 3 cut(s) 167, 400, 409
NdeII GATC 2 cut(s) 240, 596
NlaIII CATG 1 cut(s) 247
NmuCI GTSAC 2 cut(s) 326, 388
OliI CACNNNNGTG 1 cut(s) 195
PagI TCATGA 1 cut(s) 243
PciSI GCTCTTC 1 cut(s) 279
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 6 cut(s) 172, 540, 543, 615, 696, 724
PleI GAGTC 2 cut(s) 357, 441
PpsI GAGTC 2 cut(s) 357, 441
Psp124BI GAGCTC 1 cut(s) 39
Psp6I CCWGG 2 cut(s) 256, 630
PspEI GGTNACC 1 cut(s) 326
PspGI CCWGG 2 cut(s) 256, 630
PstNI CAGNNNCTG 1 cut(s) 722
PsuI RGATCY 1 cut(s) 596
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
RseI CAYNNNNRTG 3 cut(s) 195, 248, 632
SacI GAGCTC 1 cut(s) 39
SapI GCTCTTC 1 cut(s) 279
SatI GCNGC 6 cut(s) 171, 539, 542, 614, 695, 723
Sau3AI GATC 2 cut(s) 240, 596
SchI GAGTC 2 cut(s) 358, 441
ScrFI CCNGG 2 cut(s) 258, 632
SduI GDGCHC 2 cut(s) 39, 658
SfaNI GCATC 2 cut(s) 157, 720
SmiMI CAYNNNNRTG 3 cut(s) 195, 248, 632
SmlI CTYRAG 1 cut(s) 459
SmoI CTYRAG 1 cut(s) 459
Sse9I AATT 3 cut(s) 151, 162, 343
SsiI CCGC 2 cut(s) 324, 372
SspMI CTAG 2 cut(s) 92, 134
SstI GAGCTC 1 cut(s) 39
StyD4I CCNGG 2 cut(s) 256, 630
TaiI ACGT 3 cut(s) 49, 649, 692
TaqI TCGA 3 cut(s) 347, 385, 730
TasI AATT 3 cut(s) 151, 162, 343
TatI WGTACW 1 cut(s) 184
TfiI GAWTC 1 cut(s) 419
TscAI CASTG 2 cut(s) 183, 325
TseFI GTSAC 2 cut(s) 326, 388
TseI GCWGC 6 cut(s) 170, 538, 541, 613, 694, 722
Tsp45I GTSAC 2 cut(s) 326, 388
TspDTI ATGAA 3 cut(s) 260, 266, 485
TspRI CASTG 2 cut(s) 183, 325
XapI RAATTY 1 cut(s) 343
XbaI TCTAGA 1 cut(s) 91
XspI CTAG 2 cut(s) 92, 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.