Rw7G002030

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012810
Physical Location & Seq
Forward (+)
1674748 .. 1675996
1249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002030.1

Sequence Viewer

Length: 579 bp
ATGGGAAATTGCCAAGCCATAGATGCAGCAACTTTGGTGATACAACACCCAAATGGGAAGGTCGAGAAGTTCTATGGGGCAGTGAGTGCTAGTGAGGTCATGAAGATGAACCCTGGTCACTATGTTGCTCTTCTCATCTCCACCACACTCTGTCCCTCATCAAATCCCAAATCCAAAACCAATGGTCCTGATAATAATGCTGGTGATGATCATGATCGCAAGAATTCTGATATGACCAATGAGAAGAAGACTAGTTCTAGTACTGGTTCAGTTCGAATCACACGCATCAAGCTTCTCAGGCCAACTGATACTCTTGTTCTTGGCCAAGTTTACAGACTCATCACTACTCAAGAGGTTATGAAGGGTCTGTGGGCAAAGAAACAAGCAAAGGGGAAGAGATCAAACCAGTTTGAGCCACTAACAGCGGAGAGGATGCAGAGAGAGACAGTAGCAGCAGCAGCTAGCAGAAGATCTGAGGCTGCTTCTGAGCTGGACAAGGACAACAACCAGTCTGCCGCAACAGCTCGACCAAGAACATGGCAACCTTCACTGCAAAGCATCTCAGAAGCTGCGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

192

Amino Acids

20.9

Weight (kDa)

9.62

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 189 4.4e-24 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012810)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50090 AT5G50090 AT5G50090
fragaria_vesca FvH4_5g16570
malus_domestica MD06G1178700.v1.1 MD14G1185100.v1.1
prunus_persica Prupe.5G177800_v2.0.a1
pyrus_communis pycom06g15880 pycom14g15400
rosa_chinensis RchiOBHm_Chr7g0179551
rosa_multiflora Rmu_sc0005329.1_g000004
rosa_roxburghii Rroxscaffold_3G00273720
rosa_rugosa Rorug06G0425500
rosa_samantha Rh7AG025300 Rh7BG024800 Rh7CG026100 Rh7DG026100
rosa_wichuraiana Rw7G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 425, 516
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 1 cut(s) 223
AfaI GTAC 1 cut(s) 262
AhlI ACTAGT 1 cut(s) 251
AjnI CCWGG 1 cut(s) 112
AluBI AGCT 6 cut(s) 292, 461, 490, 524, 569, 576
AluI AGCT 6 cut(s) 292, 461, 490, 524, 569, 576
Alw26I GTCTC 1 cut(s) 437
AlwNI CAGNNNCTG 1 cut(s) 569
AoxI GGCC 2 cut(s) 299, 322
ApeKI GCWGC 6 cut(s) 26, 452, 455, 458, 479, 569
ApoI RAATTY 1 cut(s) 223
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 2 cut(s) 49, 215
AsuII TTCGAA 1 cut(s) 274
AsuNHI GCTAGC 1 cut(s) 461
AvaII GGWCC 1 cut(s) 185
BaeI ACNNNNGTAYC 2 cut(s) 300, 333
BalI TGGCCA 1 cut(s) 324
BbsI GAAGAC 1 cut(s) 254
BbvI GCAGC 6 cut(s) 38, 464, 466, 467, 470, 556
BciT130I CCWGG 1 cut(s) 114
BclI TGATCA 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 437
BcuI ACTAGT 1 cut(s) 251
BfaI CTAG 4 cut(s) 90, 252, 258, 462
BglII AGATCT 1 cut(s) 470
BisI GCNGC 7 cut(s) 27, 453, 456, 459, 480, 516, 570
BlsI GCNGC 7 cut(s) 28, 454, 457, 460, 481, 517, 571
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmrFI CCNGG 1 cut(s) 114
BmsI GCATC 4 cut(s) 13, 294, 423, 567
BmtI GCTAGC 1 cut(s) 465
BpiI GAAGAC 1 cut(s) 254
Bpu14I TTCGAA 1 cut(s) 274
BpuEI CTTGAG 1 cut(s) 333
BsaBI GATNNNNATC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 112
Bse1I ACTGG 3 cut(s) 268, 406, 508
Bse8I GATNNNNATC 1 cut(s) 213
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 1 cut(s) 438
BseJI GATNNNNATC 1 cut(s) 213
BseMII CTCAG 4 cut(s) 310, 465, 477, 576
BseNI ACTGG 3 cut(s) 268, 406, 508
BseXI GCAGC 6 cut(s) 38, 464, 466, 467, 470, 556
BshFI GGCC 2 cut(s) 301, 324
BslFI GGGAC 1 cut(s) 138
BsmAI GTCTC 1 cut(s) 437
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 2 cut(s) 301, 324
Bsp119I TTCGAA 1 cut(s) 274
Bsp143I GATC 4 cut(s) 208, 214, 398, 470
BspACI CCGC 2 cut(s) 425, 516
BspANI GGCC 2 cut(s) 301, 324
BspCNI CTCAG 4 cut(s) 309, 466, 478, 575
BspHI TCATGA 2 cut(s) 99, 211
BspOI GCTAGC 1 cut(s) 465
BspQI GCTCTTC 1 cut(s) 135
BspT104I TTCGAA 1 cut(s) 274
BsrI ACTGG 3 cut(s) 268, 406, 508
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 4 cut(s) 208, 214, 398, 470
Bst2UI CCWGG 1 cut(s) 114
Bst4CI ACNGT 1 cut(s) 448
Bst6I CTCTTC 2 cut(s) 135, 389
BstAPI GCANNNNNTGC 1 cut(s) 86
BstBI TTCGAA 1 cut(s) 274
BstC8I GCNNGC 2 cut(s) 463, 574
BstDEI CTNAG 4 cut(s) 296, 474, 486, 562
BstF5I GGATG 1 cut(s) 438
BstKTI GATC 4 cut(s) 211, 217, 401, 473
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 4 cut(s) 208, 214, 398, 470
BstMWI GCNNNNNNNGC 5 cut(s) 23, 86, 298, 458, 521
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 112
BstV1I GCAGC 6 cut(s) 38, 464, 466, 467, 470, 556
BstV2I GAAGAC 1 cut(s) 254
BstX2I RGATCY 1 cut(s) 470
BstXI CCANNNNNNTGG 1 cut(s) 537
BstYI RGATCY 1 cut(s) 470
BsuRI GGCC 2 cut(s) 301, 324
BtsCI GGATG 1 cut(s) 438
BtsI GCAGTG 2 cut(s) 87, 548
BtsIMutI CAGTG 2 cut(s) 87, 548
Cac8I GCNNGC 2 cut(s) 463, 574
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 2 cut(s) 99, 211
Cfr13I GGNCC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 261
CviAII CATG 3 cut(s) 100, 212, 537
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 4 cut(s) 296, 474, 486, 562
DpnI GATC 4 cut(s) 210, 216, 400, 472
DpnII GATC 4 cut(s) 208, 214, 398, 470
EaeI YGGCCR 1 cut(s) 322
Eam1104I CTCTTC 2 cut(s) 135, 389
EarI CTCTTC 2 cut(s) 135, 389
Eco47I GGWCC 1 cut(s) 185
EcoRI GAATTC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 112
FaeI CATG 3 cut(s) 103, 215, 540
FaiI YATR 8 cut(s) 20, 75, 101, 123, 213, 233, 359, 538
FaqI GGGAC 1 cut(s) 138
FatI CATG 3 cut(s) 99, 211, 536
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 7 cut(s) 27, 453, 456, 459, 480, 516, 570
FokI GGATG 1 cut(s) 445
Fsp4HI GCNGC 7 cut(s) 27, 453, 456, 459, 480, 516, 570
FspBI CTAG 4 cut(s) 90, 252, 258, 462
GluI GCNGC 7 cut(s) 27, 453, 456, 459, 480, 516, 570
HaeIII GGCC 2 cut(s) 301, 324
Hin1II CATG 3 cut(s) 103, 215, 540
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 2 cut(s) 276, 336
HphI GGTGA 2 cut(s) 49, 215
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 4 cut(s) 229, 475, 487, 565
Hpy188III TCNNGA 5 cut(s) 64, 100, 188, 212, 350
Hpy8I GTNNAC 1 cut(s) 331
HpyAV CCTTC 3 cut(s) 52, 355, 555
HpyCH4III ACNGT 1 cut(s) 448
HpyCH4V TGCA 3 cut(s) 26, 436, 553
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 86, 298, 458, 521
HpyF3I CTNAG 4 cut(s) 296, 474, 486, 562
Hsp92II CATG 3 cut(s) 103, 215, 540
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 4 cut(s) 208, 214, 398, 470
LguI GCTCTTC 1 cut(s) 135
LpnPI CCDG 9 cut(s) 99, 126, 186, 201, 249, 283, 419, 476, 521
Lsp1109I GCAGC 6 cut(s) 38, 464, 466, 467, 470, 556
LweI GCATC 4 cut(s) 13, 294, 423, 567
MaeI CTAG 4 cut(s) 90, 252, 258, 462
MaeIII GTNAC 1 cut(s) 116
MalI GATC 4 cut(s) 210, 216, 400, 472
MboI GATC 4 cut(s) 208, 214, 398, 470
MboII GAAGA 6 cut(s) 115, 122, 256, 259, 406, 480
MflI RGATCY 1 cut(s) 470
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 2 cut(s) 7, 223
MluNI TGGCCA 1 cut(s) 324
MlyI GAGTC 1 cut(s) 330
MnlI CCTC 5 cut(s) 88, 166, 346, 423, 469
Mox20I TGGCCA 1 cut(s) 324
MscI TGGCCA 1 cut(s) 324
MslI CAYNNNNRTG 2 cut(s) 51, 104
Msp20I TGGCCA 1 cut(s) 324
MspA1I CMGCKG 1 cut(s) 425
MspR9I CCNGG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 114
MwoI GCNNNNNNNGC 5 cut(s) 23, 86, 298, 458, 521
NdeII GATC 4 cut(s) 208, 214, 398, 470
NheI GCTAGC 1 cut(s) 461
NlaIII CATG 3 cut(s) 103, 215, 540
NmuCI GTSAC 1 cut(s) 116
NspV TTCGAA 1 cut(s) 274
PagI TCATGA 2 cut(s) 99, 211
PciSI GCTCTTC 1 cut(s) 135
PfeI GAWTC 1 cut(s) 276
PkrI GCNGC 7 cut(s) 28, 454, 457, 460, 481, 517, 571
PleI GAGTC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 112
PspGI CCWGG 1 cut(s) 112
PspPI GGNCC 1 cut(s) 185
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 1 cut(s) 470
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
RseI CAYNNNNRTG 2 cut(s) 51, 104
SapI GCTCTTC 1 cut(s) 135
SatI GCNGC 7 cut(s) 27, 453, 456, 459, 480, 516, 570
Sau3AI GATC 4 cut(s) 208, 214, 398, 470
Sau96I GGNCC 1 cut(s) 185
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 1 cut(s) 330
ScrFI CCNGG 1 cut(s) 114
SfaNI GCATC 4 cut(s) 13, 294, 423, 567
SfuI TTCGAA 1 cut(s) 274
SinI GGWCC 1 cut(s) 185
SmiMI CAYNNNNRTG 2 cut(s) 51, 104
SmlI CTYRAG 1 cut(s) 348
SmoI CTYRAG 1 cut(s) 348
SpeI ACTAGT 1 cut(s) 251
Sse9I AATT 2 cut(s) 7, 223
SsiI CCGC 2 cut(s) 425, 516
SspMI CTAG 4 cut(s) 90, 252, 258, 462
StyD4I CCNGG 1 cut(s) 112
TaaI ACNGT 1 cut(s) 448
TaqI TCGA 3 cut(s) 63, 274, 526
TasI AATT 2 cut(s) 7, 223
TatI WGTACW 1 cut(s) 260
TauI GCSGC 1 cut(s) 518
TfiI GAWTC 1 cut(s) 276
TscAI CASTG 2 cut(s) 87, 555
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 6 cut(s) 26, 452, 455, 458, 479, 569
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 116, 122, 374
TspRI CASTG 2 cut(s) 87, 555
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 1 cut(s) 223
XspI CTAG 4 cut(s) 90, 252, 258, 462
ZrmI AGTACT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.