Rh7AG025300

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0012810
Physical Location & Seq
Forward (+)
1668814 .. 1670101
1288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG025300.1

Sequence Viewer

Length: 525 bp
ATGGGAAATTGCCAAGCCATAGATGCAGCAACTTTGGTGATACAACACCCAAATGGGAAGGTCGAGAAGTTCTATGGGGCAGTGAGTGCTAGTGAGGTCATGAAGATGAACCCTGGTCACTATGTTGCTCTTCTCATCTCCACCACACTCTGTCCCTCATCAAATCCCAAATCCAAAACCAATGGTCCTGATAATAATGCTGGTGATGATCATGATCACAAGAATTCTGATATGACCAATGAGAAGAAGACTAGTTCTAGTACTGGTTCAGTTCGAATCACACGCATCAAGCTTCTCAGGCCAACTGATACTCTTGTTCTTGGCCAAGTTTACAGACTCATCACCACTCAAGACACCACCACCGACCTAGAACATCAACCTCATCGTCAGGGAGCACTAGTAGTAGTCAGTCTGCCGCAACAGCTCGACCAAGAACATGGCAACCTTCACTGCAAAGCATCTCAGAAGCTGCGAGCTGATGATCATGAAGACGATGATGCACGTGTTCAATCTTCGGCCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

174

Amino Acids

18.97

Weight (kDa)

6.28

Isoelectric Point (pI)

24.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 130 2.4e-18 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012810)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50090 AT5G50090 AT5G50090
fragaria_vesca FvH4_5g16570
malus_domestica MD06G1178700.v1.1 MD14G1185100.v1.1
prunus_persica Prupe.5G177800_v2.0.a1
pyrus_communis pycom06g15880 pycom14g15400
rosa_chinensis RchiOBHm_Chr7g0179551
rosa_multiflora Rmu_sc0005329.1_g000004
rosa_roxburghii Rroxscaffold_3G00273720
rosa_rugosa Rorug06G0425500
rosa_samantha Rh7AG025300 Rh7BG024800 Rh7CG026100 Rh7DG026100
rosa_wichuraiana Rw7G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 416
AcoI YGGCCR 2 cut(s) 322, 516
AcsI RAATTY 1 cut(s) 223
AcvI CACGTG 1 cut(s) 503
AfaI GTAC 1 cut(s) 262
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 1 cut(s) 509
AhlI ACTAGT 2 cut(s) 251, 397
AjnI CCWGG 1 cut(s) 112
AluBI AGCT 4 cut(s) 292, 424, 469, 476
AluI AGCT 4 cut(s) 292, 424, 469, 476
Alw21I GWGCWC 1 cut(s) 397
AlwNI CAGNNNCTG 1 cut(s) 469
AoxI GGCC 3 cut(s) 299, 322, 516
ApeKI GCWGC 2 cut(s) 26, 469
ApoI RAATTY 1 cut(s) 223
AspS9I GGNCC 1 cut(s) 185
AsuHPI GGTGA 3 cut(s) 49, 215, 334
AsuII TTCGAA 1 cut(s) 274
AvaII GGWCC 1 cut(s) 185
BaeI ACNNNNGTAYC 2 cut(s) 300, 333
BalI TGGCCA 1 cut(s) 324
BbrPI CACGTG 1 cut(s) 503
BbsI GAAGAC 2 cut(s) 254, 495
Bbv12I GWGCWC 1 cut(s) 397
BbvI GCAGC 2 cut(s) 38, 456
BciT130I CCWGG 1 cut(s) 114
BclI TGATCA 3 cut(s) 208, 214, 481
BcuI ACTAGT 2 cut(s) 251, 397
BfaI CTAG 5 cut(s) 90, 252, 258, 368, 398
BisI GCNGC 3 cut(s) 27, 416, 470
BlsI GCNGC 3 cut(s) 28, 417, 471
BmcAI AGTACT 1 cut(s) 262
Bme1390I CCNGG 1 cut(s) 114
Bme18I GGWCC 1 cut(s) 185
BmgT120I GGNCC 1 cut(s) 185
BmrFI CCNGG 1 cut(s) 114
BmsI GCATC 4 cut(s) 13, 294, 467, 487
BpiI GAAGAC 2 cut(s) 254, 495
Bpu14I TTCGAA 1 cut(s) 274
BpuEI CTTGAG 1 cut(s) 333
BsaAI YACGTR 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 112
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bse1I ACTGG 1 cut(s) 268
Bse8I GATNNNNATC 1 cut(s) 213
BseBI CCWGG 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 112
BseJI GATNNNNATC 1 cut(s) 213
BseMII CTCAG 2 cut(s) 310, 476
BseNI ACTGG 1 cut(s) 268
BseXI GCAGC 2 cut(s) 38, 456
BshFI GGCC 3 cut(s) 301, 324, 518
BsiHKAI GWGCWC 1 cut(s) 397
BslFI GGGAC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 138
BsnI GGCC 3 cut(s) 301, 324, 518
Bsp119I TTCGAA 1 cut(s) 274
Bsp1286I GDGCHC 1 cut(s) 397
Bsp143I GATC 3 cut(s) 208, 214, 481
BspACI CCGC 1 cut(s) 416
BspANI GGCC 3 cut(s) 301, 324, 518
BspCNI CTCAG 2 cut(s) 309, 475
BspHI TCATGA 3 cut(s) 99, 211, 484
BspQI GCTCTTC 1 cut(s) 135
BspT104I TTCGAA 1 cut(s) 274
BsrI ACTGG 1 cut(s) 268
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 3 cut(s) 208, 214, 481
Bst2UI CCWGG 1 cut(s) 114
Bst6I CTCTTC 1 cut(s) 135
BstAPI GCANNNNNTGC 1 cut(s) 86
BstBAI YACGTR 1 cut(s) 503
BstBI TTCGAA 1 cut(s) 274
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 2 cut(s) 296, 462
BstKTI GATC 3 cut(s) 211, 217, 484
BstMBI GATC 3 cut(s) 208, 214, 481
BstMWI GCNNNNNNNGC 4 cut(s) 23, 86, 298, 421
BstNI CCWGG 1 cut(s) 114
BstSCI CCNGG 1 cut(s) 112
BstV1I GCAGC 2 cut(s) 38, 456
BstV2I GAAGAC 2 cut(s) 254, 495
BstXI CCANNNNNNTGG 1 cut(s) 437
BsuRI GGCC 3 cut(s) 301, 324, 518
BtsI GCAGTG 2 cut(s) 87, 448
BtsIMutI CAGTG 2 cut(s) 87, 448
Cac8I GCNNGC 1 cut(s) 474
CaiI CAGNNNCTG 1 cut(s) 469
CciI TCATGA 3 cut(s) 99, 211, 484
Cfr13I GGNCC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 261
CviAII CATG 5 cut(s) 100, 212, 437, 485, 522
CviJI RGCY 8 cut(s) 17, 292, 301, 324, 424, 469, 476, 518
CviKI_1 RGCY 8 cut(s) 17, 292, 301, 324, 424, 469, 476, 518
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 2 cut(s) 296, 462
DpnI GATC 3 cut(s) 210, 216, 483
DpnII GATC 3 cut(s) 208, 214, 481
EaeI YGGCCR 2 cut(s) 322, 516
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
Eco47I GGWCC 1 cut(s) 185
Eco72I CACGTG 1 cut(s) 503
EcoRI GAATTC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 112
FaeI CATG 5 cut(s) 103, 215, 440, 488, 525
FaiI YATR 9 cut(s) 20, 75, 101, 123, 213, 233, 438, 486, 523
FaqI GGGAC 1 cut(s) 138
FatI CATG 5 cut(s) 99, 211, 436, 484, 521
FbaI TGATCA 3 cut(s) 208, 214, 481
Fnu4HI GCNGC 3 cut(s) 27, 416, 470
Fsp4HI GCNGC 3 cut(s) 27, 416, 470
FspBI CTAG 5 cut(s) 90, 252, 258, 368, 398
GluI GCNGC 3 cut(s) 27, 416, 470
HaeIII GGCC 3 cut(s) 301, 324, 518
Hin1II CATG 5 cut(s) 103, 215, 440, 488, 525
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 2 cut(s) 276, 336
HphI GGTGA 3 cut(s) 49, 215, 334
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 2 cut(s) 229, 465
Hpy188III TCNNGA 6 cut(s) 64, 100, 188, 212, 350, 485
Hpy8I GTNNAC 1 cut(s) 331
HpyAV CCTTC 2 cut(s) 52, 455
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 3 cut(s) 26, 453, 500
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 86, 298, 421
HpyF3I CTNAG 2 cut(s) 296, 462
HpySE526I ACGT 1 cut(s) 502
Hsp92II CATG 5 cut(s) 103, 215, 440, 488, 525
Ksp22I TGATCA 3 cut(s) 208, 214, 481
Kzo9I GATC 3 cut(s) 208, 214, 481
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 392
LpnPI CCDG 7 cut(s) 99, 126, 186, 201, 249, 283, 374
Lsp1109I GCAGC 2 cut(s) 38, 456
LweI GCATC 4 cut(s) 13, 294, 467, 487
MaeI CTAG 5 cut(s) 90, 252, 258, 368, 398
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 1 cut(s) 116
MalI GATC 3 cut(s) 210, 216, 483
MboI GATC 3 cut(s) 208, 214, 481
MboII GAAGA 6 cut(s) 115, 122, 256, 259, 500, 504
MhlI GDGCHC 1 cut(s) 397
MlsI TGGCCA 1 cut(s) 324
MluCI AATT 2 cut(s) 7, 223
MluNI TGGCCA 1 cut(s) 324
MlyI GAGTC 1 cut(s) 330
MnlI CCTC 3 cut(s) 88, 166, 390
Mox20I TGGCCA 1 cut(s) 324
MscI TGGCCA 1 cut(s) 324
MslI CAYNNNNRTG 2 cut(s) 51, 104
Msp20I TGGCCA 1 cut(s) 324
MspR9I CCNGG 1 cut(s) 114
MvaI CCWGG 1 cut(s) 114
MwoI GCNNNNNNNGC 4 cut(s) 23, 86, 298, 421
NdeII GATC 3 cut(s) 208, 214, 481
NlaIII CATG 5 cut(s) 103, 215, 440, 488, 525
NmuCI GTSAC 1 cut(s) 116
NspV TTCGAA 1 cut(s) 274
PagI TCATGA 3 cut(s) 99, 211, 484
PciSI GCTCTTC 1 cut(s) 135
PfeI GAWTC 1 cut(s) 276
PkrI GCNGC 3 cut(s) 28, 417, 471
PleI GAGTC 1 cut(s) 330
PmaCI CACGTG 1 cut(s) 503
PmlI CACGTG 1 cut(s) 503
PpsI GAGTC 1 cut(s) 330
Ppu21I YACGTR 1 cut(s) 503
Psp6I CCWGG 1 cut(s) 112
PspCI CACGTG 1 cut(s) 503
PspGI CCWGG 1 cut(s) 112
PspPI GGNCC 1 cut(s) 185
PstNI CAGNNNCTG 1 cut(s) 469
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
RseI CAYNNNNRTG 2 cut(s) 51, 104
SapI GCTCTTC 1 cut(s) 135
SatI GCNGC 3 cut(s) 27, 416, 470
Sau3AI GATC 3 cut(s) 208, 214, 481
Sau96I GGNCC 1 cut(s) 185
ScaI AGTACT 1 cut(s) 262
SchI GAGTC 1 cut(s) 330
ScrFI CCNGG 1 cut(s) 114
SduI GDGCHC 1 cut(s) 397
SfaNI GCATC 4 cut(s) 13, 294, 467, 487
SfuI TTCGAA 1 cut(s) 274
SinI GGWCC 1 cut(s) 185
SmiMI CAYNNNNRTG 2 cut(s) 51, 104
SmlI CTYRAG 1 cut(s) 348
SmoI CTYRAG 1 cut(s) 348
SpeI ACTAGT 2 cut(s) 251, 397
Sse9I AATT 2 cut(s) 7, 223
SsiI CCGC 1 cut(s) 416
SspMI CTAG 5 cut(s) 90, 252, 258, 368, 398
StyD4I CCNGG 1 cut(s) 112
TaiI ACGT 1 cut(s) 505
TaqI TCGA 3 cut(s) 63, 274, 426
TasI AATT 2 cut(s) 7, 223
TatI WGTACW 1 cut(s) 260
TauI GCSGC 1 cut(s) 418
TfiI GAWTC 1 cut(s) 276
TscAI CASTG 2 cut(s) 87, 455
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 2 cut(s) 26, 469
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 116, 122, 501
TspRI CASTG 2 cut(s) 87, 455
VpaK11BI GGWCC 1 cut(s) 185
XapI RAATTY 1 cut(s) 223
XspI CTAG 5 cut(s) 90, 252, 258, 368, 398
ZrmI AGTACT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.