AT5G50130

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006773
Physical Location & Seq
Forward (+)
20389915 .. 20393306
3392 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50130.2

Sequence Viewer

Length: 1020 bp
ATGAAGGGAACTCTAAGATACTTAGCAGGAATCGCTGGGCCAAATGGGTTTGGCTCAAGATCCACAGCTGAACAAGTTACACAACATAGCTTTTTTCCTTGTTCACATCTCACTGCCATAATCACTGGAGGAACGTCTGGGATCGGTGCGGAAACTGCGAGAGTGTTAGCAAAAAGAGGGGTGAGAGTAGTGATGGCAGTAAGAGATATGAAGAAAGCAGAAATGGTGAAGGAGAGAATAATAAGAGAGAATCCAGAAGCTGACATTATATTATTCGAGATTGATCTGAGCTCTCTATCTTCCGTTGCCAGATTCTGCTCTCAATTTCTTTCCCAAGATCTTCCCCTCAACATACTCATAAACAACGCAGGAGTTTTCTCTCCAAATCTTGAATTCTCCGAAGAAAAGATTGAGCTGACATTTGCTACAAACTTTTTAGGACATTACTTATTAACAGAAATGCTGATAGAGAAAATGATAGATACGGCAGAGAAGAGTGGAATCGAAGGACGAATCATTAACCTTTCCTCTGTGATTCACAATTGGGTCAAGCCTGATTGTTTCTCCTTCCCTAAATTGCTCCATCCCATTAGTAGGTATAACGGGACAAGGGCATATGCTCAGTCAAAGTTGGCTACAATTTTACATGCGAAAGCGTTGAGCAAGCAATTAAAGGACAGGAATGCAAACGTGACGATAAATGCAGTCCATCCAGGAATTGTTAAAACTGGAATTATCAGAGCACACAAGGGTCTTTTTACAGATTCTCTGTTTTTGATAGCTTCGAAGCTGCTAAAATCTATAAGCCAGGGCGCAGCTACAACATGTTATGTGGCATTGAGCAACGAAACCAAAGGGTTAAGTGGAAAGTATTTTGCAGACTGCAATGAGACCAATTGCTCAGATTTAGCGAATGATGAGTATGTGGCTCTCAAGCTCTGCACCCAAAGCCGTGCCTTAATCCATGATCATTTGCATGAATTTCAAATACATGCCCTTCCTAATTTATGTTTTTTGTAA

Protein Analysis

339

Amino Acids

37.28

Weight (kDa)

8.7

Isoelectric Point (pI)

30.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 38 - 179 1.5e-27 short chain dehydrogenase
KR PF08659 38 - 161 6.2e-09 KR domain
Epimerase PF01370 40 - 223 2.8e-07 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 46 - 250 2.9e-18 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 149
AclWI GGATC 2 cut(s) 54, 149
AcsI RAATTY 2 cut(s) 392, 980
AfiI CCNNNNNNNGG 1 cut(s) 594
AflIII ACRYGT 1 cut(s) 824
AgsI TTSAA 2 cut(s) 392, 986
AjnI CCWGG 2 cut(s) 712, 807
AluBI AGCT 9 cut(s) 68, 90, 260, 291, 415, 782, 790, 818, 937
AluI AGCT 9 cut(s) 68, 90, 260, 291, 415, 782, 790, 818, 937
Alw21I GWGCWC 2 cut(s) 293, 745
Alw26I GTCTC 1 cut(s) 884
AlwI GGATC 2 cut(s) 54, 149
AlwNI CAGNNNCTG 2 cut(s) 260, 315
AoxI GGCC 1 cut(s) 38
ApeKI GCWGC 2 cut(s) 790, 815
ApoI RAATTY 2 cut(s) 392, 980
AspLEI GCGC 1 cut(s) 815
AspS9I GGNCC 1 cut(s) 38
AsuHPI GGTGA 2 cut(s) 193, 238
AsuII TTCGAA 1 cut(s) 785
BanII GRGCYC 1 cut(s) 293
Bbv12I GWGCWC 2 cut(s) 293, 745
BbvI GCAGC 2 cut(s) 777, 827
BccI CCATC 3 cut(s) 187, 591, 717
BceAI ACGGC 2 cut(s) 501, 936
BciT130I CCWGG 2 cut(s) 714, 809
BclI TGATCA 1 cut(s) 967
BcoDI GTCTC 1 cut(s) 884
BglII AGATCT 1 cut(s) 337
BisI GCNGC 2 cut(s) 791, 816
BlsI GCNGC 2 cut(s) 792, 817
Bme1390I CCNGG 2 cut(s) 714, 809
BmgT120I GGNCC 1 cut(s) 38
BmrFI CCNGG 2 cut(s) 714, 809
BpmI CTGGAG 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 785
BpuEI CTTGAG 2 cut(s) 40, 917
BsaI GGTCTC 1 cut(s) 884
BsaJI CCNNGG 1 cut(s) 808
Bsc4I CCNNNNNNNGG 1 cut(s) 594
Bse1I ACTGG 2 cut(s) 130, 733
Bse3DI GCAATG 1 cut(s) 892
BseBI CCWGG 2 cut(s) 714, 809
BseDI CCNNGG 1 cut(s) 808
BseGI GGATG 2 cut(s) 583, 709
BseLI CCNNNNNNNGG 1 cut(s) 594
BseMI GCAATG 1 cut(s) 892
BseMII CTCAG 3 cut(s) 278, 635, 915
BseNI ACTGG 2 cut(s) 130, 733
BseXI GCAGC 2 cut(s) 777, 827
BseYI CCCAGC 1 cut(s) 35
BsgI GTGCAG 1 cut(s) 925
BshFI GGCC 1 cut(s) 40
BsiHKAI GWGCWC 2 cut(s) 293, 745
BslFI GGGAC 1 cut(s) 619
BslI CCNNNNNNNGG 1 cut(s) 594
BsmAI GTCTC 1 cut(s) 884
BsmFI GGGAC 1 cut(s) 619
BsmI GAATGC 1 cut(s) 688
BsnI GGCC 1 cut(s) 40
Bso31I GGTCTC 1 cut(s) 884
Bsp119I TTCGAA 1 cut(s) 785
Bsp1286I GDGCHC 2 cut(s) 293, 745
Bsp143I GATC 5 cut(s) 59, 141, 283, 337, 967
BspACI CCGC 1 cut(s) 149
BspANI GGCC 1 cut(s) 40
BspCNI CTCAG 3 cut(s) 279, 634, 914
BspPI GGATC 2 cut(s) 54, 149
BspT104I TTCGAA 1 cut(s) 785
BspTNI GGTCTC 1 cut(s) 884
BsrDI GCAATG 1 cut(s) 892
BsrI ACTGG 2 cut(s) 130, 733
BssECI CCNNGG 1 cut(s) 808
BssMI GATC 5 cut(s) 59, 141, 283, 337, 967
Bst2UI CCWGG 2 cut(s) 714, 809
Bst6I CTCTTC 1 cut(s) 488
BstBI TTCGAA 1 cut(s) 785
BstC8I GCNNGC 1 cut(s) 665
BstDEI CTNAG 5 cut(s) 14, 22, 287, 621, 901
BstF5I GGATG 2 cut(s) 583, 709
BstHHI GCGC 1 cut(s) 815
BstKTI GATC 5 cut(s) 62, 144, 286, 340, 970
BstMAI GTCTC 1 cut(s) 884
BstMBI GATC 5 cut(s) 59, 141, 283, 337, 967
BstMWI GCNNNNNNNGC 3 cut(s) 32, 155, 948
BstNI CCWGG 2 cut(s) 714, 809
BstNSI RCATGY 3 cut(s) 650, 828, 995
BstSCI CCNGG 2 cut(s) 712, 807
BstV1I GCAGC 2 cut(s) 777, 827
BstX2I RGATCY 2 cut(s) 59, 337
BstYI RGATCY 2 cut(s) 59, 337
BsuRI GGCC 1 cut(s) 40
BtsCI GGATG 2 cut(s) 583, 709
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 2 cut(s) 111, 123
Cac8I GCNNGC 1 cut(s) 665
CaiI CAGNNNCTG 2 cut(s) 260, 315
CfoI GCGC 1 cut(s) 815
Cfr13I GGNCC 1 cut(s) 38
CviAII CATG 5 cut(s) 647, 825, 965, 977, 992
DdeI CTNAG 5 cut(s) 14, 22, 287, 621, 901
DpnI GATC 5 cut(s) 61, 143, 285, 339, 969
DpnII GATC 5 cut(s) 59, 141, 283, 337, 967
Eam1104I CTCTTC 1 cut(s) 488
EarI CTCTTC 1 cut(s) 488
Ecl136II GAGCTC 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 293
Eco31I GGTCTC 1 cut(s) 884
Eco53kI GAGCTC 1 cut(s) 291
EcoICRI GAGCTC 1 cut(s) 291
EcoRI GAATTC 1 cut(s) 392
EcoRII CCWGG 2 cut(s) 712, 807
EcoT38I GRGCYC 1 cut(s) 293
FaeI CATG 5 cut(s) 650, 828, 968, 980, 995
FaqI GGGAC 1 cut(s) 619
FatI CATG 5 cut(s) 646, 824, 964, 976, 991
FauNDI CATATG 1 cut(s) 616
FbaI TGATCA 1 cut(s) 967
Fnu4HI GCNGC 2 cut(s) 791, 816
FokI GGATG 2 cut(s) 570, 696
FriOI GRGCYC 1 cut(s) 293
Fsp4HI GCNGC 2 cut(s) 791, 816
GlaI GCGC 1 cut(s) 814
GluI GCNGC 2 cut(s) 791, 816
GsaI CCCAGC 1 cut(s) 39
GsuI CTGGAG 1 cut(s) 147
HaeIII GGCC 1 cut(s) 40
HhaI GCGC 1 cut(s) 815
Hin1II CATG 5 cut(s) 650, 828, 968, 980, 995
Hin6I GCGC 1 cut(s) 813
HinP1I GCGC 1 cut(s) 813
HinfI GANTC 7 cut(s) 30, 250, 312, 501, 513, 535, 764
HphI GGTGA 2 cut(s) 193, 238
Hpy166II GTNNAC 1 cut(s) 104
Hpy188I TCNGA 4 cut(s) 288, 400, 740, 904
Hpy188III TCNNGA 4 cut(s) 57, 254, 277, 389
Hpy8I GTNNAC 1 cut(s) 104
HpyAV CCTTC 4 cut(s) 223, 500, 577, 1007
HpyCH4IV ACGT 2 cut(s) 134, 690
HpyCH4V TGCA 6 cut(s) 686, 704, 878, 885, 942, 976
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 155, 948
HpyF3I CTNAG 5 cut(s) 14, 22, 287, 621, 901
HpySE526I ACGT 2 cut(s) 134, 690
Hsp92II CATG 5 cut(s) 650, 828, 968, 980, 995
HspAI GCGC 1 cut(s) 813
Ksp22I TGATCA 1 cut(s) 967
Kzo9I GATC 5 cut(s) 59, 141, 283, 337, 967
LmnI GCTCC 1 cut(s) 585
Lsp1109I GCAGC 2 cut(s) 777, 827
MaeII ACGT 2 cut(s) 134, 690
MaeIII GTNAC 2 cut(s) 76, 691
MalI GATC 5 cut(s) 61, 143, 285, 339, 969
MboI GATC 5 cut(s) 59, 141, 283, 337, 967
MboII GAAGA 5 cut(s) 223, 291, 332, 413, 505
MfeI CAATTG 2 cut(s) 541, 895
MflI RGATCY 2 cut(s) 59, 337
MhlI GDGCHC 2 cut(s) 293, 745
MnlI CCTC 4 cut(s) 122, 170, 356, 538
MseI TTAA 6 cut(s) 452, 519, 671, 723, 860, 959
MslI CAYNNNNRTG 1 cut(s) 975
MspA1I CMGCKG 1 cut(s) 68
MspR9I CCNGG 2 cut(s) 714, 809
MunI CAATTG 2 cut(s) 541, 895
Mva1269I GAATGC 1 cut(s) 688
MvaI CCWGG 2 cut(s) 714, 809
MwoI GCNNNNNNNGC 3 cut(s) 32, 155, 948
NdeI CATATG 1 cut(s) 616
NdeII GATC 5 cut(s) 59, 141, 283, 337, 967
NlaIII CATG 5 cut(s) 650, 828, 968, 980, 995
NmuCI GTSAC 1 cut(s) 691
NspI RCATGY 3 cut(s) 650, 828, 995
NspV TTCGAA 1 cut(s) 785
PciI ACATGT 1 cut(s) 824
PctI GAATGC 1 cut(s) 688
PfeI GAWTC 7 cut(s) 30, 250, 312, 501, 513, 535, 764
PfoI TCCNGGA 1 cut(s) 712
PkrI GCNGC 2 cut(s) 792, 817
PscI ACATGT 1 cut(s) 824
Psp124BI GAGCTC 1 cut(s) 293
Psp6I CCWGG 2 cut(s) 712, 807
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 2 cut(s) 712, 807
PspPI GGNCC 1 cut(s) 38
PstNI CAGNNNCTG 2 cut(s) 260, 315
PsuI RGATCY 2 cut(s) 59, 337
PvuII CAGCTG 1 cut(s) 68
RseI CAYNNNNRTG 1 cut(s) 975
SacI GAGCTC 1 cut(s) 293
SaqAI TTAA 6 cut(s) 452, 519, 671, 723, 860, 959
SatI GCNGC 2 cut(s) 791, 816
Sau3AI GATC 5 cut(s) 59, 141, 283, 337, 967
Sau96I GGNCC 1 cut(s) 38
ScrFI CCNGG 2 cut(s) 714, 809
SduI GDGCHC 2 cut(s) 293, 745
SfuI TTCGAA 1 cut(s) 785
SmiMI CAYNNNNRTG 1 cut(s) 975
SmlI CTYRAG 2 cut(s) 55, 932
SmoI CTYRAG 2 cut(s) 55, 932
SsiI CCGC 1 cut(s) 149
SstI GAGCTC 1 cut(s) 293
StyD4I CCNGG 2 cut(s) 712, 807
TaiI ACGT 2 cut(s) 137, 693
TaqI TCGA 3 cut(s) 276, 504, 785
TfiI GAWTC 7 cut(s) 30, 250, 312, 501, 513, 535, 764
Tru1I TTAA 6 cut(s) 452, 519, 671, 723, 860, 959
Tru9I TTAA 6 cut(s) 452, 519, 671, 723, 860, 959
TscAI CASTG 2 cut(s) 118, 130
TseFI GTSAC 1 cut(s) 691
TseI GCWGC 2 cut(s) 790, 815
Tsp45I GTSAC 1 cut(s) 691
TspDTI ATGAA 3 cut(s) 17, 224, 993
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 2 cut(s) 118, 130
XapI RAATTY 2 cut(s) 392, 980
XceI RCATGY 3 cut(s) 650, 828, 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.