MD06G1170000.v1.1

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006773
Physical Location & Seq
Forward (+)
31065960 .. 31069628
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1170000.v1.1.491

Sequence Viewer

Length: 993 bp
ATGAAGGAGACACTAAGGTACTTGGCAGGAATTGCAGGGCCAAGTGGTTATGGCTCAAAATCAACTGCTGAGCAAGTCACTCGGGATTGTTCTTCCTCAGTCCCTTCTCAACTCACTGCCATAATCACTGGAGCAACATCAGGGATCGGAGCTGAAACGGCAAGAGTGCTGGCAAAGAGAGGAGTGAGGGTTGTGATTCCGGCCAGAGATTTATCGAAGGCGGCGGAAGTGAAGGAGGGGATTCAAAGGGAGAGTCCAGAGGCTGAGGTTATAATCTTGGAGATTGACCTGAGCTCACTTGCTTCTGTTGATCGATTTTGTTCTGAGTTCTTAGCTCTAAGACTTCCCCTCAACATCCTCATAAACAATGGTGGGATGTTTTCTCAGGCTTTGGAATATTCTGAAGACAAAATTGAGATGACATTTGCTACAAATTACTTGGGACACCATTTTTTGACAGAAAAGCTGCTGGAAAAAATGGTGGAAACAGCAGCTCAGACCGGCATCCGAGGGAGAATAATCAACGTTTCTTCTGTGATCCACAGCTGGGTTAAGAGAGATGACTTCTCCTTCAACCAATTGCTCAACCCTAACAATTATAATGGTACAAAAGCATATGCTCAATCCAAATTGGCTAACATATTGCATGCCAAGGAACTGGCAAGACAGCTGAAGGCAAGAAATGCAAGAGTAACGATCAATGCCATACATCCGGGAATTGTGAAGACGGGAATTATGAGAGCTCATAAGGGCTTTATCACAGATTCTGTGTTCTTCATCGCGTCCAAGTTACTAAAATCGACATCTCAGGGTGCTTCGACAACGTGCTATGTTGCTTTAAGCCCGGAGCTGGAAGGAGTGAGTGGGAAATACTTTGCAGACTGCAACGAGAGCACCTGCTCGAAACTAGCAAACGATGAACCTGAAGCTCGTAAGTTGTGGAAGCAGACTCGTCTGCTAATCCATAGACGCCTTTCTCAACCAACAGCGTAA

Protein Analysis

331

Amino Acids

36.12

Weight (kDa)

9.28

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 39 - 180 4.4e-27 short chain dehydrogenase
KR PF08659 39 - 158 3.5e-07 KR domain
Epimerase PF01370 41 - 224 1e-05 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 44 - 290 2.3e-19 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 272, 600
AarI CACCTGC 1 cut(s) 905
Acc36I ACCTGC 1 cut(s) 905
AccII CGCG 1 cut(s) 782
AciI CCGC 2 cut(s) 221, 224
AclI AACGTT 1 cut(s) 525
AclWI GGATC 2 cut(s) 152, 532
AcoI YGGCCR 1 cut(s) 201
AcuI CTGAAG 3 cut(s) 423, 692, 945
AcyI GRCGYC 1 cut(s) 970
AfaI GTAC 2 cut(s) 20, 607
AfiI CCNNNNNNNGG 2 cut(s) 547, 850
AgsI TTSAA 2 cut(s) 245, 574
Alw21I GWGCWC 3 cut(s) 296, 745, 896
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 2 cut(s) 152, 532
AlwNI CAGNNNCTG 2 cut(s) 263, 767
Ama87I CYCGRG 1 cut(s) 81
AoxI GGCC 2 cut(s) 38, 201
ApeKI GCWGC 2 cut(s) 466, 491
ArsI GACNNNNNNTTYG 2 cut(s) 238, 270
AspS9I GGNCC 1 cut(s) 38
AsuC2I CCSGG 2 cut(s) 714, 845
AvaI CYCGRG 1 cut(s) 81
BanII GRGCYC 2 cut(s) 296, 745
BbsI GAAGAC 2 cut(s) 411, 731
Bbv12I GWGCWC 3 cut(s) 296, 745, 896
BbvCI CCTCAGC 1 cut(s) 264
BbvI GCAGC 2 cut(s) 453, 503
BceAI ACGGC 1 cut(s) 174
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BcnI CCSGG 2 cut(s) 714, 845
BcoDI GTCTC 1 cut(s) 2
BfaI CTAG 1 cut(s) 908
BfuAI ACCTGC 1 cut(s) 905
BisI GCNGC 3 cut(s) 222, 467, 492
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 3 cut(s) 223, 468, 493
Bme1390I CCNGG 2 cut(s) 714, 845
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 1 cut(s) 38
BmrFI CCNGG 2 cut(s) 714, 845
BmsI GCATC 1 cut(s) 513
BpiI GAAGAC 2 cut(s) 411, 731
BpmI CTGGAG 1 cut(s) 150
Bpu10I CCTNAGC 2 cut(s) 264, 290
Bpu1102I GCTNAGC 1 cut(s) 69
BpuMI CCSGG 2 cut(s) 714, 845
Bsa29I ATCGAT 1 cut(s) 313
BsaHI GRCGYC 1 cut(s) 970
BsaJI CCNNGG 2 cut(s) 508, 651
BsaXI ACNNNNNCTCC 2 cut(s) 174, 204
Bsc4I CCNNNNNNNGG 2 cut(s) 547, 850
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 2 cut(s) 133, 663
BseCI ATCGAT 1 cut(s) 313
BseDI CCNNGG 2 cut(s) 508, 651
BseGI GGATG 4 cut(s) 354, 381, 504, 709
BseLI CCNNNNNNNGG 2 cut(s) 547, 850
BseMII CTCAG 8 cut(s) 60, 111, 255, 281, 315, 398, 509, 821
BseNI ACTGG 2 cut(s) 133, 663
BseRI GAGGAG 1 cut(s) 195
BseXI GCAGC 2 cut(s) 453, 503
BseYI CCCAGC 1 cut(s) 546
Bsh1236I CGCG 1 cut(s) 782
BshFI GGCC 2 cut(s) 40, 203
BshVI ATCGAT 1 cut(s) 313
BsiHKAI GWGCWC 3 cut(s) 296, 745, 896
BsiHKCI CYCGRG 1 cut(s) 81
BsiSI CCGG 4 cut(s) 200, 501, 713, 845
BslFI GGGAC 2 cut(s) 86, 456
BslI CCNNNNNNNGG 2 cut(s) 547, 850
BsmAI GTCTC 1 cut(s) 2
BsmFI GGGAC 2 cut(s) 86, 456
BsnI GGCC 2 cut(s) 40, 203
BsoBI CYCGRG 1 cut(s) 81
Bsp1286I GDGCHC 3 cut(s) 296, 745, 896
Bsp143I GATC 4 cut(s) 144, 310, 537, 696
Bsp1720I GCTNAGC 1 cut(s) 69
BspACI CCGC 2 cut(s) 221, 224
BspANI GGCC 2 cut(s) 40, 203
BspCNI CTCAG 8 cut(s) 61, 110, 256, 282, 316, 397, 508, 820
BspDI ATCGAT 1 cut(s) 313
BspFNI CGCG 1 cut(s) 782
BspMI ACCTGC 1 cut(s) 905
BspPI GGATC 2 cut(s) 152, 532
BsrFI RCCGGY 1 cut(s) 500
BsrI ACTGG 2 cut(s) 133, 663
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 2 cut(s) 508, 651
BssMI GATC 4 cut(s) 144, 310, 537, 696
BssNI GRCGYC 1 cut(s) 970
BssT1I CCWWGG 1 cut(s) 651
BstACI GRCGYC 1 cut(s) 970
BstAPI GCANNNNNTGC 2 cut(s) 32, 683
BstC8I GCNNGC 2 cut(s) 171, 648
BstF5I GGATG 4 cut(s) 354, 381, 504, 709
BstFNI CGCG 1 cut(s) 782
BstKTI GATC 4 cut(s) 147, 313, 540, 699
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 4 cut(s) 144, 310, 537, 696
BstMWI GCNNNNNNNGC 4 cut(s) 32, 158, 683, 891
BstNSI RCATGY 1 cut(s) 650
BstSCI CCNGG 2 cut(s) 712, 843
BstUI CGCG 1 cut(s) 782
BstV1I GCAGC 2 cut(s) 453, 503
BstV2I GAAGAC 2 cut(s) 411, 731
BstXI CCANNNNNNTGG 1 cut(s) 658
Bsu15I ATCGAT 1 cut(s) 313
BsuRI GGCC 2 cut(s) 40, 203
BsuTUI ATCGAT 1 cut(s) 313
BtgZI GCGATG 1 cut(s) 763
BtsCI GGATG 4 cut(s) 354, 381, 504, 709
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 2 cut(s) 114, 126
BveI ACCTGC 1 cut(s) 905
Cac8I GCNNGC 2 cut(s) 171, 648
CaiI CAGNNNCTG 2 cut(s) 263, 767
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 1 cut(s) 38
ClaI ATCGAT 1 cut(s) 313
CseI GACGC 2 cut(s) 771, 978
Csp6I GTAC 2 cut(s) 19, 606
CviAII CATG 1 cut(s) 647
CviQI GTAC 2 cut(s) 19, 606
DpnI GATC 4 cut(s) 146, 312, 539, 698
DpnII GATC 4 cut(s) 144, 310, 537, 696
EaeI YGGCCR 1 cut(s) 201
EciI GGCGGA 1 cut(s) 239
Ecl136II GAGCTC 2 cut(s) 294, 743
Eco130I CCWWGG 1 cut(s) 651
Eco24I GRGCYC 2 cut(s) 296, 745
Eco53kI GAGCTC 2 cut(s) 294, 743
Eco57I CTGAAG 3 cut(s) 423, 692, 945
Eco88I CYCGRG 1 cut(s) 81
EcoICRI GAGCTC 2 cut(s) 294, 743
EcoT14I CCWWGG 1 cut(s) 651
EcoT38I GRGCYC 2 cut(s) 296, 745
ErhI CCWWGG 1 cut(s) 651
FaeI CATG 1 cut(s) 650
FaqI GGGAC 2 cut(s) 86, 456
FatI CATG 1 cut(s) 646
FauNDI CATATG 1 cut(s) 616
Fnu4HI GCNGC 3 cut(s) 222, 467, 492
FokI GGATG 4 cut(s) 341, 388, 491, 696
FriOI GRGCYC 2 cut(s) 296, 745
Fsp4HI GCNGC 3 cut(s) 222, 467, 492
FspBI CTAG 1 cut(s) 908
GluI GCNGC 3 cut(s) 222, 467, 492
GsaI CCCAGC 1 cut(s) 550
GsuI CTGGAG 1 cut(s) 150
HaeIII GGCC 2 cut(s) 40, 203
HapII CCGG 4 cut(s) 200, 501, 713, 845
HgaI GACGC 2 cut(s) 771, 978
Hin1I GRCGYC 1 cut(s) 970
Hin1II CATG 1 cut(s) 650
HinfI GANTC 5 cut(s) 196, 241, 253, 764, 949
HpaII CCGG 4 cut(s) 200, 501, 713, 845
Hpy188I TCNGA 5 cut(s) 149, 325, 403, 498, 509
Hpy188III TCNNGA 2 cut(s) 83, 257
HpyAV CCTTC 6 cut(s) 114, 211, 226, 580, 667, 848
HpyCH4IV ACGT 2 cut(s) 525, 824
HpyCH4V TGCA 5 cut(s) 35, 646, 686, 878, 885
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 158, 683, 891
HpySE526I ACGT 2 cut(s) 525, 824
Hsp92I GRCGYC 1 cut(s) 970
Hsp92II CATG 1 cut(s) 650
Kzo9I GATC 4 cut(s) 144, 310, 537, 696
LmnI GCTCC 3 cut(s) 131, 149, 847
Lsp1109I GCAGC 2 cut(s) 453, 503
LweI GCATC 1 cut(s) 513
MaeI CTAG 1 cut(s) 908
MaeII ACGT 2 cut(s) 525, 824
MaeIII GTNAC 3 cut(s) 76, 691, 789
MalI GATC 4 cut(s) 146, 312, 539, 698
MboI GATC 4 cut(s) 144, 310, 537, 696
MboII GAAGA 5 cut(s) 84, 416, 522, 736, 766
MfeI CAATTG 1 cut(s) 578
MhlI GDGCHC 3 cut(s) 296, 745, 896
MluCI AATT 8 cut(s) 30, 411, 433, 578, 595, 629, 717, 732
MlyI GAGTC 2 cut(s) 262, 943
MnlI CCTC 9 cut(s) 106, 173, 180, 229, 253, 259, 359, 368, 503
MseI TTAA 2 cut(s) 552, 839
MspA1I CMGCKG 2 cut(s) 546, 670
MspI CCGG 4 cut(s) 200, 501, 713, 845
MspR9I CCNGG 2 cut(s) 714, 845
MunI CAATTG 1 cut(s) 578
MvnI CGCG 1 cut(s) 782
MwoI GCNNNNNNNGC 4 cut(s) 32, 158, 683, 891
NciI CCSGG 2 cut(s) 714, 845
NdeI CATATG 1 cut(s) 616
NdeII GATC 4 cut(s) 144, 310, 537, 696
NlaIII CATG 1 cut(s) 650
NmuCI GTSAC 1 cut(s) 76
NspI RCATGY 1 cut(s) 650
PaeI GCATGC 1 cut(s) 650
PaqCI CACCTGC 1 cut(s) 905
PfeI GAWTC 3 cut(s) 196, 241, 764
PfoI TCCNGGA 1 cut(s) 712
PkrI GCNGC 3 cut(s) 223, 468, 493
PleI GAGTC 2 cut(s) 261, 943
PpsI GAGTC 2 cut(s) 261, 943
PsiI TTATAA 2 cut(s) 272, 600
Psp124BI GAGCTC 2 cut(s) 296, 745
Psp1406I AACGTT 1 cut(s) 525
PspFI CCCAGC 1 cut(s) 546
PspPI GGNCC 1 cut(s) 38
PstNI CAGNNNCTG 2 cut(s) 263, 767
PvuII CAGCTG 2 cut(s) 546, 670
RsaI GTAC 2 cut(s) 20, 607
RsaNI GTAC 2 cut(s) 19, 606
SacI GAGCTC 2 cut(s) 296, 745
SaqAI TTAA 2 cut(s) 552, 839
SatI GCNGC 3 cut(s) 222, 467, 492
Sau3AI GATC 4 cut(s) 144, 310, 537, 696
Sau96I GGNCC 1 cut(s) 38
SchI GAGTC 2 cut(s) 262, 943
ScrFI CCNGG 2 cut(s) 714, 845
SduI GDGCHC 3 cut(s) 296, 745, 896
SfaNI GCATC 1 cut(s) 513
SphI GCATGC 1 cut(s) 650
Sse9I AATT 8 cut(s) 30, 411, 433, 578, 595, 629, 717, 732
SsiI CCGC 2 cut(s) 221, 224
SspI AATATT 1 cut(s) 398
SspMI CTAG 1 cut(s) 908
SstI GAGCTC 2 cut(s) 296, 745
StyD4I CCNGG 2 cut(s) 712, 843
StyI CCWWGG 1 cut(s) 651
TaiI ACGT 2 cut(s) 528, 827
TaqI TCGA 5 cut(s) 215, 313, 800, 818, 902
TasI AATT 8 cut(s) 30, 411, 433, 578, 595, 629, 717, 732
TauI GCSGC 1 cut(s) 224
TfiI GAWTC 3 cut(s) 196, 241, 764
Tru1I TTAA 2 cut(s) 552, 839
Tru9I TTAA 2 cut(s) 552, 839
TscAI CASTG 2 cut(s) 121, 133
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 2 cut(s) 466, 491
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 3 cut(s) 17, 766, 933
TspRI CASTG 2 cut(s) 121, 133
XceI RCATGY 1 cut(s) 650
XspI CTAG 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.