AT5G50170

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011354
Physical Location & Seq
Forward (+)
20420951 .. 20425604
4654 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50170.1

Sequence Viewer

Length: 3084 bp
ATGAGGCTCTACGTCTACATATTACAAGCCAAAGATTTACCAGCGAAAGAGACATTCGCTAAGCTTCACGTTGGGAGACACAAGTCTAAAACGAGAGTCGCGAGAGATACTTCGTCACCGATCTGGAACGAGGAATTCGTTTTTCGAATCAGTGATGTCGACGAAGGTGATGATGTCGTTGTATCGATTCTTCACCATGAGCAGCAAGATCATCAGTCTATTGTTTCCACCGGTTTAATTGGTAAAGTTCGGATTCCGCTTACTTCCGTTGCTGCTGAAGAGAATCAAACTTTGTTGCCGACTTGGTTTGTCATTGAGAAACCTAGTGATGGCAAGTTCGTTAACATTGAATGTGGGAAAATTCTTCTGAGTTTGTCTTTGCAAGGGAAATGGGAAAGTACCTCTGGTGAGAAGGTTCTTAATGATAAGCAGGATATTATTAACTTGGAAGGTGTGAAAGAACTCGAAGGATCGCCTAAAGATTTGATCTCCTCCAGAGATGGGAAACGGCGTAAACACCATGATGGGAAGCACATAATGAAGAATATTGTGAACCACATTGACAAGCTTTTTCATAAGAAAGAGGAGATCTCTAAGAGACTCCATGATGAGTCTTCAGTAGGACAAAGTGTAAACTCAAATTATGAGGATGCCACTGACCAATGTTCTTCCTCTGCTACTTGTACTGGCTTTGAGGAAGGTCTTGACCTGATGCAGTCTAGTGATAGCGAAAGAGAAGAAATGCCTGAGAATCTTACAGGGGGAGTTCTTGTTGATCAGAAATATTTAGTCTCACCCTGTGAACTTAACAAATTTCTTTTTACGCCAAGTTCGCAATTTAGGAAAGAATTGGCTGAACTTCAAGGTCTATCAGATGTACAGGAAGGGCCTTGGACAATGATGCAAGAAGATACTCCTCGTCTAACACGGGTTGTTACATACATGAGAGCTGCAACGAAGATGGTCAAAGCAGTAAAAGCAACAGAAAATCAAGTTTACCGGAAAGCTAGCGGGAAGCAGTTTGCTGTATTTGTTAGTGTAAGCACACCAGATGTTCCCTACGGAAATACCTTCAAAATAGAGTTGCTTTACAAGATACTACCTGAAACAGAGCCAACTGCTGGTGGGGAAGCTTCTCGACTGATCATATCATGGGGGATTCAATTTAGTCAAAGTACTATAATGAAAGGTATGATTGAAGGAGGTGCTAGGCAAGGGCTGAAAGAAAGCTTTGAACAATTTTCTAATCTCCTGGCTAAGACTTATAAAACTCTTGATCCGGCTGTTGTATTGGACAAGGAGCAGGTTATAGCAACAGTGCAGTCAGAGCCAAAGACTGATTTGAAATCAGCTTTTCTATACTTCTGGAGTTCCTCTGTCATCTGTGCTGTTCTTTTGTCTGTATATGTTGTAGTTCATATGCTGCACTGTGAGCCCAGCAAAATCCAAGGGTTTGAATTCTATGGGCTTGATTTGCCTGATAGTTTTGGAGAGCTATTCAGTAGTGGAATACTTGTTCTTCTTCTGGAGCGTGTTTACATAATGACTGTGCACTTCGTACAAGCTAGATTACATCGAGGAAGAGATCAGGGAGTCAAAGCAAATGGTAAGGGGTGGATTCTGACAATAGCTTTAATTAAGGGAACAAATTTGGCTTCAGTGGAAGCAACAGAGCTTTTTGATCCCTATGTTGTTTTCACTTGCAATGGGAAAACAAGAACAAGTTCTGTCAAACTTCAAGCCCAGGATCCTCAGTGGAATGAGGTAATCGAGTTTGATGCAATGGAGGAACCACCATCGGTATTAGATGTGGAGGTATTTGATTTTGATGGTCCATTTGACCAGGGGGCTTCTCTTGGTCATGCAGAGATCAATTTCTTGAAACATACAGCCGATGAACTTGCTGACTTGAGTGTTGCTCTAGTTGGTAATCATGCTCAAGCGTCTCAGTCGAAGTTACAATTGAGAATTTTCCTGGAAAACAAGAACGGGGTTGAAACAATGAAGGATTACTTGAGTAAGGTGGAAAAGGAAGTCGGCAAGAAGTTGAACATTCGGTCACCACAGAAGAACTCTGCTTTCCAGAAACTGTTTGGTTTGCCACATGAAGAATTTCTTCTCAAAGAATATACTTGTTACTTGAAGAGAAAACTTCCGGTGCAGGGCAAGCTCTTTCTGTCTGCTAGAATTGTGGCGTTCTACTCAAATGTATTTGGACACAAGACAAAGTTTTATTTCCTCTGGGAAGATATTGATGACATCCAAGTGCTTCCCCCAACTTTTGCTTCATTAGGAAGCCCTTTGCTACTCATTATTCTGAAAAAGAACCGAGGTCTTGATGCAAAGCATGGAGCTAAATCTCAGGATGATGAAGGAAGACTTTCGTTCTATTTCCAGTCATTTGTATCATTTGATGCCACCAGCAGGACAATCATGGCTTTGTGGAAAACAAGGACGTTAAGTGTGGATCATAGAGCACAAATAGTAGAAGAAGACCAAGATGTGGCAGATCCTTTCCTCTTGCCTGAAGCTGTTACAGTTGTTTCAGATGCGGATGCATTAATGATGTCAAAAGTGTACACGTGTGATCTACCTTGCGATGTAGAATTAGTAATGAAGATCTTTGGTGGAGGAGAATTGGAGCGTAAGATCATGGAGAAATCTGGATGTTTGAGTTACGCTAGTACAACATGGGAATCTAAAAAACCGGGAGTCTACGAAAGACGGCTTTCTTACAAGTATAACCACTATGTGTCTGTGTTTGGAGGTGGAGTCACATGTGCACAACAGAAATCTCCAGCTCCAAATGATGAAGGCTGGATCTTGAATGAAATTGTGGCTCTCCACGATGTTCCATTCGGAGATCATTTTCGTGTTCATATAAGGTACGAGGTGAAGAAAGCAGGAGTTGATTGTAAAACAAGTAAGTGTGAAGTGTATTTGAAGATCAGGTGGCTCAAAACAATAAAGTTTGAGCAAAGAATTAGCAAAAGTATAATGGAGAAATTTAGGAATCGATTCAAAGTTATATTTGATTTGTTCCAGAAAGAATCTGTTGCAAATTCATCTTTGACACTTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1027

Amino Acids

115.86

Weight (kDa)

6.28

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 97 9.5e-16 C2 domain
VASt PF16016 257 - 406 1.3e-30 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 540 - 628 4.2e-13 C2 domain
GRAM PF02893 693 - 777 5.4e-17 GRAM domain
VASt PF16016 859 - 1001 5.9e-23 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50170
fragaria_vesca FvH4_5g06260 FvH4_5g06260 FvH4_5g06260
malus_domestica MD06G1166100.v1.1
prunus_persica Prupe.5G166000_v2.0.a1
pyrus_communis pycom06g14860
rosa_chinensis RchiOBHm_Chr7g0192601
rosa_laevigata RLG00000004348
rosa_multiflora Rmu_sc0004161.1_g000002
rosa_roxburghii Rroxscaffold_3G00262850
rosa_rugosa Rorug07G0002600 Rorug07G0002700 Rorug07G0002800
rosa_samantha Rh7AG127000 Rh7BG129600 Rh7CG131100 Rh7CG132000 Rh7DG130600
rosa_wichuraiana Rw7G010940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1266
Acc36I ACCTGC 1 cut(s) 1294
AccB7I CCANNNNNTGG 2 cut(s) 1121, 2503
AccI GTMKAC 3 cut(s) 15, 159, 2715
AccII CGCG 1 cut(s) 101
AciI CCGC 3 cut(s) 257, 1011, 2552
AclWI GGATC 8 cut(s) 478, 1271, 1676, 1742, 1755, 2475, 2504, 2828
AcsI RAATTY 9 cut(s) 134, 360, 812, 1457, 1648, 1968, 2111, 3005, 3061
AcuI CTGAAG 4 cut(s) 297, 600, 1641, 2547
AcvI CACGTG 1 cut(s) 2583
AdeI CACNNNGTG 2 cut(s) 800, 2752
AfaI GTAC 8 cut(s) 400, 685, 879, 1177, 1560, 2579, 2686, 2888
AfiI CCNNNNNNNGG 6 cut(s) 329, 501, 1121, 2162, 2424, 2503
AflIII ACRYGT 3 cut(s) 2580, 2582, 2777
AgeI ACCGGT 1 cut(s) 230
AjnI CCWGG 4 cut(s) 1251, 1743, 1842, 1974
AjuI GAANNNNNNNTTGG 2 cut(s) 2269, 2301
AloI GAACNNNNNNTCC 2 cut(s) 1500, 1532
Alw21I GWGCWC 3 cut(s) 1554, 2479, 2785
Alw26I GTCTC 5 cut(s) 44, 70, 592, 796, 1950
Alw44I GTGCAC 2 cut(s) 1550, 2781
AlwI GGATC 8 cut(s) 478, 1271, 1676, 1742, 1755, 2475, 2504, 2828
AlwNI CAGNNNCTG 1 cut(s) 2089
AoxI GGCC 1 cut(s) 887
ApaLI GTGCAC 2 cut(s) 1550, 2781
ApeKI GCWGC 4 cut(s) 202, 272, 950, 1423
ApoI RAATTY 9 cut(s) 134, 360, 812, 1457, 1648, 1968, 2111, 3005, 3061
AseI ATTAAT 1 cut(s) 2561
AsiGI ACCGGT 1 cut(s) 230
Asp700I GAANNNNTTC 5 cut(s) 1723, 2112, 2115, 2380, 3017
AspS9I GGNCC 2 cut(s) 887, 1832
AsuC2I CCSGG 1 cut(s) 2709
AsuHPI GGTGA 7 cut(s) 108, 179, 185, 419, 786, 2052, 2905
AsuII TTCGAA 1 cut(s) 145
AsuNHI GCTAGC 1 cut(s) 1007
AvaII GGWCC 1 cut(s) 1832
BaeGI GKGCMC 2 cut(s) 1554, 2785
BamHI GGATCC 1 cut(s) 1747
BanII GRGCYC 1 cut(s) 1437
BarI GAAGNNNNNNTAC 2 cut(s) 174, 206
BbrPI CACGTG 1 cut(s) 2583
BbsI GAAGAC 3 cut(s) 606, 2383, 2499
Bbv12I GWGCWC 3 cut(s) 1554, 2479, 2785
BbvI GCAGC 4 cut(s) 214, 259, 937, 1410
BccI CCATC 6 cut(s) 323, 494, 518, 955, 1804, 1823
BceAI ACGGC 2 cut(s) 524, 2741
BcgI CGANNNNNNTGC 4 cut(s) 1760, 1794, 1883, 1917
BciT130I CCWGG 4 cut(s) 1253, 1745, 1844, 1976
BclI TGATCA 2 cut(s) 775, 1143
BcnI CCSGG 1 cut(s) 2709
BcoDI GTCTC 5 cut(s) 44, 70, 592, 796, 1950
BfaI CTAG 8 cut(s) 324, 720, 1008, 1209, 1566, 1922, 2184, 2682
BfuAI ACCTGC 1 cut(s) 1294
BglII AGATCT 2 cut(s) 588, 2619
BisI GCNGC 4 cut(s) 203, 273, 951, 1424
BlpI GCTNAGC 1 cut(s) 60
BlsI GCNGC 4 cut(s) 204, 274, 952, 1425
BmcAI AGTACT 1 cut(s) 1177
Bme1390I CCNGG 5 cut(s) 1253, 1745, 1844, 1976, 2709
Bme18I GGWCC 1 cut(s) 1832
BmgT120I GGNCC 2 cut(s) 887, 1832
BmiI GGNNCC 2 cut(s) 1749, 1791
BmrFI CCNGG 5 cut(s) 1253, 1745, 1844, 1976, 2709
BmsI GCATC 8 cut(s) 640, 702, 891, 1768, 2329, 2404, 2539, 2545
BmtI GCTAGC 1 cut(s) 1011
BoxI GACNNNNGTC 1 cut(s) 82
BpiI GAAGAC 3 cut(s) 606, 2383, 2499
BplI GAGNNNNNCTC 4 cut(s) 575, 607, 1903, 1935
BpmI CTGGAG 4 cut(s) 478, 1387, 1547, 2781
Bpu1102I GCTNAGC 1 cut(s) 60
Bpu14I TTCGAA 1 cut(s) 145
BpuEI CTTGAG 3 cut(s) 1923, 1930, 2035
BpuMI CCSGG 1 cut(s) 2709
Bsa29I ATCGAT 2 cut(s) 185, 3016
BsaAI YACGTR 1 cut(s) 2583
BsaJI CCNNGG 5 cut(s) 890, 1447, 1743, 1843, 2329
BsaWI WCCGGW 3 cut(s) 230, 999, 2155
BsaXI ACNNNNNCTCC 4 cut(s) 756, 786, 2757, 2787
Bsc4I CCNNNNNNNGG 6 cut(s) 329, 501, 1121, 2162, 2424, 2503
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 2 cut(s) 691, 2395
Bse3DI GCAATG 2 cut(s) 1711, 1788
BseBI CCWGG 4 cut(s) 1253, 1745, 1844, 1976
BseCI ATCGAT 2 cut(s) 185, 3016
BseDI CCNNGG 5 cut(s) 890, 1447, 1743, 1843, 2329
BseGI GGATG 5 cut(s) 655, 2259, 2371, 2560, 2672
BseLI CCNNNNNNNGG 6 cut(s) 329, 501, 1121, 2162, 2424, 2503
BseMI GCAATG 2 cut(s) 1711, 1788
BseMII CTCAG 5 cut(s) 359, 738, 1766, 1961, 2375
BseNI ACTGG 2 cut(s) 691, 2395
BseRI GAGGAG 4 cut(s) 481, 599, 906, 2646
BseSI GKGCMC 2 cut(s) 1554, 2785
BseXI GCAGC 4 cut(s) 214, 259, 937, 1410
BseYI CCCAGC 1 cut(s) 1436
BsgI GTGCAG 3 cut(s) 1340, 1409, 2180
Bsh1236I CGCG 1 cut(s) 101
BshFI GGCC 1 cut(s) 889
BshTI ACCGGT 1 cut(s) 230
BshVI ATCGAT 2 cut(s) 185, 3016
BsiHKAI GWGCWC 3 cut(s) 1554, 2479, 2785
BsiSI CCGG 5 cut(s) 231, 1000, 1280, 2156, 2708
BslI CCNNNNNNNGG 6 cut(s) 329, 501, 1121, 2162, 2424, 2503
BsmAI GTCTC 5 cut(s) 44, 70, 592, 796, 1950
BsmBI CGTCTC 1 cut(s) 1950
BsnI GGCC 1 cut(s) 889
Bsp119I TTCGAA 1 cut(s) 145
Bsp1286I GDGCHC 4 cut(s) 1437, 1554, 2479, 2785
Bsp1407I TGTACA 2 cut(s) 877, 2577
Bsp1720I GCTNAGC 1 cut(s) 60
Bsp68I TCGCGA 1 cut(s) 101
BspACI CCGC 3 cut(s) 257, 1011, 2552
BspANI GGCC 1 cut(s) 889
BspCNI CTCAG 5 cut(s) 360, 739, 1765, 1960, 2374
BspDI ATCGAT 2 cut(s) 185, 3016
BspFNI CGCG 1 cut(s) 101
BspLI GGNNCC 2 cut(s) 1749, 1791
BspMI ACCTGC 1 cut(s) 1294
BspOI GCTAGC 1 cut(s) 1011
BspPI GGATC 8 cut(s) 478, 1271, 1676, 1742, 1755, 2475, 2504, 2828
BspT104I TTCGAA 1 cut(s) 145
BsrDI GCAATG 2 cut(s) 1711, 1788
BsrFI RCCGGY 1 cut(s) 230
BsrGI TGTACA 2 cut(s) 877, 2577
BsrI ACTGG 2 cut(s) 691, 2395
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 5 cut(s) 890, 1447, 1743, 1843, 2329
BssT1I CCWWGG 2 cut(s) 890, 1447
Bst2UI CCWGG 4 cut(s) 1253, 1745, 1844, 1976
Bst4CI ACNGT 5 cut(s) 1318, 1430, 1549, 2091, 2539
Bst6I CTCTTC 3 cut(s) 273, 1576, 2138
BstAUI TGTACA 2 cut(s) 877, 2577
BstBAI YACGTR 1 cut(s) 2583
BstBI TTCGAA 1 cut(s) 145
BstC8I GCNNGC 2 cut(s) 1009, 2168
BstDEI CTNAG 8 cut(s) 60, 368, 594, 747, 1257, 1752, 1947, 2361
BstEII GGTNACC 1 cut(s) 2058
BstF5I GGATG 5 cut(s) 655, 2259, 2371, 2560, 2672
BstFNI CGCG 1 cut(s) 101
BstMAI GTCTC 5 cut(s) 44, 70, 592, 796, 1950
BstMWI GCNNNNNNNGC 6 cut(s) 832, 977, 1327, 1432, 1474, 2167
BstNI CCWGG 4 cut(s) 1253, 1745, 1844, 1976
BstNSI RCATGY 1 cut(s) 2781
BstPAI GACNNNNGTC 1 cut(s) 82
BstPI GGTNACC 1 cut(s) 2058
BstSCI CCNGG 5 cut(s) 1251, 1743, 1842, 1974, 2707
BstSLI GKGCMC 2 cut(s) 1554, 2785
BstUI CGCG 1 cut(s) 101
BstV1I GCAGC 4 cut(s) 214, 259, 937, 1410
BstV2I GAAGAC 3 cut(s) 606, 2383, 2499
BstX2I RGATCY 5 cut(s) 588, 1747, 2509, 2619, 2820
BstYI RGATCY 5 cut(s) 588, 1747, 2509, 2619, 2820
Bsu15I ATCGAT 2 cut(s) 185, 3016
BsuRI GGCC 1 cut(s) 889
BsuTUI ATCGAT 2 cut(s) 185, 3016
BtgZI GCGATG 1 cut(s) 2613
BtsCI GGATG 5 cut(s) 655, 2259, 2371, 2560, 2672
BtsIMutI CAGTG 6 cut(s) 157, 654, 1323, 1426, 1665, 1760
BtuMI TCGCGA 1 cut(s) 101
BveI ACCTGC 1 cut(s) 1294
Cac8I GCNNGC 2 cut(s) 1009, 2168
CaiI CAGNNNCTG 1 cut(s) 2089
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 2 cut(s) 887, 1832
ClaI ATCGAT 2 cut(s) 185, 3016
CseI GACGC 1 cut(s) 1932
Csp6I GTAC 8 cut(s) 399, 684, 878, 1176, 1559, 2578, 2685, 2887
CspAI ACCGGT 1 cut(s) 230
CviQI GTAC 8 cut(s) 399, 684, 878, 1176, 1559, 2578, 2685, 2887
DdeI CTNAG 8 cut(s) 60, 368, 594, 747, 1257, 1752, 1947, 2361
DraIII CACNNNGTG 2 cut(s) 800, 2752
Eam1104I CTCTTC 3 cut(s) 273, 1576, 2138
EarI CTCTTC 3 cut(s) 273, 1576, 2138
Eco130I CCWWGG 2 cut(s) 890, 1447
Eco24I GRGCYC 1 cut(s) 1437
Eco47I GGWCC 1 cut(s) 1832
Eco57I CTGAAG 4 cut(s) 297, 600, 1641, 2547
Eco72I CACGTG 1 cut(s) 2583
Eco91I GGTNACC 1 cut(s) 2058
EcoO109I RGGNCCY 1 cut(s) 887
EcoO65I GGTNACC 1 cut(s) 2058
EcoRI GAATTC 2 cut(s) 134, 1457
EcoRII CCWGG 4 cut(s) 1251, 1743, 1842, 1974
EcoT14I CCWWGG 2 cut(s) 890, 1447
EcoT22I ATGCAT 1 cut(s) 2560
EcoT38I GRGCYC 1 cut(s) 1437
ErhI CCWWGG 2 cut(s) 890, 1447
Esp3I CGTCTC 1 cut(s) 1950
FalI AAGNNNNNCTT 6 cut(s) 1997, 2029, 2100, 2132, 2364, 2396
FauI CCCGC 1 cut(s) 1004
FauNDI CATATG 1 cut(s) 1419
FbaI TGATCA 2 cut(s) 775, 1143
FblI GTMKAC 3 cut(s) 15, 159, 2715
Fnu4HI GCNGC 4 cut(s) 203, 273, 951, 1424
FokI GGATG 5 cut(s) 662, 2246, 2378, 2567, 2679
FriOI GRGCYC 1 cut(s) 1437
Fsp4HI GCNGC 4 cut(s) 203, 273, 951, 1424
FspBI CTAG 8 cut(s) 324, 720, 1008, 1209, 1566, 1922, 2184, 2682
GluI GCNGC 4 cut(s) 203, 273, 951, 1424
GsaI CCCAGC 1 cut(s) 1440
GsuI CTGGAG 4 cut(s) 478, 1387, 1547, 2781
HaeIII GGCC 1 cut(s) 889
HapII CCGG 5 cut(s) 231, 1000, 1280, 2156, 2708
HgaI GACGC 1 cut(s) 1932
HincII GTYRAC 2 cut(s) 160, 343
HindII GTYRAC 2 cut(s) 160, 343
HindIII AAGCTT 4 cut(s) 62, 566, 1131, 1228
HpaI GTTAAC 1 cut(s) 343
HpaII CCGG 5 cut(s) 231, 1000, 1280, 2156, 2708
HphI GGTGA 7 cut(s) 108, 179, 185, 419, 786, 2052, 2905
Hpy99I CGWCG 1 cut(s) 164
HpyCH4III ACNGT 5 cut(s) 1318, 1430, 1549, 2091, 2539
HpyCH4IV ACGT 4 cut(s) 12, 69, 2456, 2582
HpyF10VI GCNNNNNNNGC 6 cut(s) 832, 977, 1327, 1432, 1474, 2167
HpyF3I CTNAG 8 cut(s) 60, 368, 594, 747, 1257, 1752, 1947, 2361
HpySE526I ACGT 4 cut(s) 12, 69, 2456, 2582
Ksp22I TGATCA 2 cut(s) 775, 1143
KspAI GTTAAC 1 cut(s) 343
LmnI GCTCC 5 cut(s) 1300, 1528, 2351, 2641, 2806
Lsp1109I GCAGC 4 cut(s) 214, 259, 937, 1410
LweI GCATC 8 cut(s) 640, 702, 891, 1768, 2329, 2404, 2539, 2545
MaeI CTAG 8 cut(s) 324, 720, 1008, 1209, 1566, 1922, 2184, 2682
MaeII ACGT 4 cut(s) 12, 69, 2456, 2582
MaeIII GTNAC 8 cut(s) 114, 934, 1956, 2058, 2135, 2533, 2675, 2773
MfeI CAATTG 1 cut(s) 1961
MflI RGATCY 5 cut(s) 588, 1747, 2509, 2619, 2820
MhlI GDGCHC 4 cut(s) 1437, 1554, 2479, 2785
MlyI GAGTC 6 cut(s) 105, 594, 620, 1602, 2721, 2781
Mph1103I ATGCAT 1 cut(s) 2560
MroXI GAANNNNTTC 5 cut(s) 1723, 2112, 2115, 2380, 3017
MseI TTAA 9 cut(s) 236, 342, 420, 441, 807, 1634, 1638, 2459, 2561
MslI CAYNNNNRTG 3 cut(s) 522, 2264, 2871
MspI CCGG 5 cut(s) 231, 1000, 1280, 2156, 2708
MspR9I CCNGG 5 cut(s) 1253, 1745, 1844, 1976, 2709
MunI CAATTG 1 cut(s) 1961
MvaI CCWGG 4 cut(s) 1253, 1745, 1844, 1976
MvnI CGCG 1 cut(s) 101
MwoI GCNNNNNNNGC 6 cut(s) 832, 977, 1327, 1432, 1474, 2167
NciI CCSGG 1 cut(s) 2709
NdeI CATATG 1 cut(s) 1419
NheI GCTAGC 1 cut(s) 1007
NlaIV GGNNCC 2 cut(s) 1749, 1791
NmuCI GTSAC 3 cut(s) 114, 2058, 2773
NruI TCGCGA 1 cut(s) 101
NsiI ATGCAT 1 cut(s) 2560
NspI RCATGY 1 cut(s) 2781
NspV TTCGAA 1 cut(s) 145
PacI TTAATTAA 1 cut(s) 1638
PciI ACATGT 1 cut(s) 2777
PcsI WCGNNNNNNNCGW 2 cut(s) 98, 135
PdmI GAANNNNTTC 5 cut(s) 1723, 2112, 2115, 2380, 3017
PflMI CCANNNNNTGG 2 cut(s) 1121, 2503
PfoI TCCNGGA 1 cut(s) 1974
PinAI ACCGGT 1 cut(s) 230
PkrI GCNGC 4 cut(s) 204, 274, 952, 1425
PleI GAGTC 6 cut(s) 104, 594, 619, 1601, 2720, 2780
PmaCI CACGTG 1 cut(s) 2583
PmlI CACGTG 1 cut(s) 2583
PpsI GAGTC 6 cut(s) 104, 594, 619, 1601, 2720, 2780
Ppu21I YACGTR 1 cut(s) 2583
PscI ACATGT 1 cut(s) 2777
PshAI GACNNNNGTC 1 cut(s) 82
PshBI ATTAAT 1 cut(s) 2561
PsiI TTATAA 1 cut(s) 1266
Psp6I CCWGG 4 cut(s) 1251, 1743, 1842, 1974
PspCI CACGTG 1 cut(s) 2583
PspEI GGTNACC 1 cut(s) 2058
PspFI CCCAGC 1 cut(s) 1436
PspGI CCWGG 4 cut(s) 1251, 1743, 1842, 1974
PspN4I GGNNCC 2 cut(s) 1749, 1791
PspPI GGNCC 2 cut(s) 887, 1832
PstNI CAGNNNCTG 1 cut(s) 2089
PsuI RGATCY 5 cut(s) 588, 1747, 2509, 2619, 2820
RruI TCGCGA 1 cut(s) 101
RsaI GTAC 8 cut(s) 400, 685, 879, 1177, 1560, 2579, 2686, 2888
RsaNI GTAC 8 cut(s) 399, 684, 878, 1176, 1559, 2578, 2685, 2887
RseI CAYNNNNRTG 3 cut(s) 522, 2264, 2871
SalI GTCGAC 1 cut(s) 158
SaqAI TTAA 9 cut(s) 236, 342, 420, 441, 807, 1634, 1638, 2459, 2561
SatI GCNGC 4 cut(s) 203, 273, 951, 1424
Sau96I GGNCC 2 cut(s) 887, 1832
ScaI AGTACT 1 cut(s) 1177
SchI GAGTC 6 cut(s) 105, 594, 620, 1602, 2721, 2781
ScrFI CCNGG 5 cut(s) 1253, 1745, 1844, 1976, 2709
SduI GDGCHC 4 cut(s) 1437, 1554, 2479, 2785
SfaNI GCATC 8 cut(s) 640, 702, 891, 1768, 2329, 2404, 2539, 2545
SfuI TTCGAA 1 cut(s) 145
SinI GGWCC 1 cut(s) 1832
SmiMI CAYNNNNRTG 3 cut(s) 522, 2264, 2871
SmlI CTYRAG 3 cut(s) 1909, 1938, 2014
SmoI CTYRAG 3 cut(s) 1909, 1938, 2014
SsiI CCGC 3 cut(s) 257, 1011, 2552
SspI AATATT 2 cut(s) 547, 785
SspMI CTAG 8 cut(s) 324, 720, 1008, 1209, 1566, 1922, 2184, 2682
StyD4I CCNGG 5 cut(s) 1251, 1743, 1842, 1974, 2707
StyI CCWWGG 2 cut(s) 890, 1447
TaaI ACNGT 5 cut(s) 1318, 1430, 1549, 2091, 2539
TaiI ACGT 4 cut(s) 15, 72, 2459, 2585
TaqI TCGA 9 cut(s) 145, 159, 185, 465, 1138, 1576, 1770, 1952, 3016
TaqII GACCGA 1 cut(s) 2046
TatI WGTACW 5 cut(s) 683, 877, 1175, 2577, 2684
Tru1I TTAA 9 cut(s) 236, 342, 420, 441, 807, 1634, 1638, 2459, 2561
Tru9I TTAA 9 cut(s) 236, 342, 420, 441, 807, 1634, 1638, 2459, 2561
TscAI CASTG 6 cut(s) 157, 661, 1323, 1433, 1665, 1760
TseFI GTSAC 3 cut(s) 114, 2058, 2773
TseI GCWGC 4 cut(s) 202, 272, 950, 1423
Tsp45I GTSAC 3 cut(s) 114, 2058, 2773
TspGWI ACGGA 2 cut(s) 256, 1077
TspRI CASTG 6 cut(s) 157, 661, 1323, 1433, 1665, 1760
Van91I CCANNNNNTGG 2 cut(s) 1121, 2503
VneI GTGCAC 2 cut(s) 1550, 2781
VpaK11BI GGWCC 1 cut(s) 1832
VspI ATTAAT 1 cut(s) 2561
XapI RAATTY 9 cut(s) 134, 360, 812, 1457, 1648, 1968, 2111, 3005, 3061
XceI RCATGY 1 cut(s) 2781
XcmI CCANNNNNNNNNTGG 1 cut(s) 2090
XmiI GTMKAC 3 cut(s) 15, 159, 2715
XmnI GAANNNNTTC 5 cut(s) 1723, 2112, 2115, 2380, 3017
XspI CTAG 8 cut(s) 324, 720, 1008, 1209, 1566, 1922, 2184, 2682
ZrmI AGTACT 1 cut(s) 1177
Zsp2I ATGCAT 1 cut(s) 2560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.