Rw7G010940

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011354
Physical Location & Seq
Reverse (-)
10187581 .. 10193186
5606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G010940.1

Sequence Viewer

Length: 3066 bp
ATGAGGCTTTACGTGTATGTGATGGAAGCTCATGACTTGGTTGTGAAGGACTCTTGCTTTGTGGAGCTTCAGGTTGGGAAGCACAAGTCCAAGACTAGGATTTTGAGGAACACCAACAACCCTGCTTGGAATGAAGAGTTTGTGTTCAGGGTGTATGATATGAATGAGGAGCTACTTGTGTCTGTTTTTCACCAGGATGATGACTCTAATGGTCTGTTTAATGGGGCGGAGTTAGTCGTCCGGGTTCGGATTCCGGTGGGGTCTGTTGCTGCTGAGAATAATAGTACCTTGCCGCCCGCATGGTATTCTTTTGAAAGGCCCAAGTCCGGGAAGTTCATCAATACAGATTGTGGAAAAATTCTTCTCACTCTATCGCTGCATGAAAAAGGCCATGATGCCGCTGCTAATGACCTCCCTTGTTTGCACTCAAATATAACTGAGGAGCCTAAAGAGATTGACAGTCCATTTGTATCATCTAATGGTGTCTTATGTTCCAAATCTCCATGTGGAAAGATACCGGGAGGAAAACAATGGATGAAGGCTCTTAGGAGACGTATGGACAGGCTTCTTCGTAAGAATGATGAAGCTTCAAGAACTGATGATTCCTCAGAGCTGTCTAGTTCAGTATCTGATTATGAAGATTGCATGGAGGAACAGCATTCTAATTGTAGCTTTGAAAAGTCTCTTGAAATGATGCTGTCAAGTGAGAGCGAACAAAAAATGCCAGAGAATCTTCCGGGGGGAATTCTTATTGATCAGGCTTATGTTGTTTCACCAAGTGATCTTAATGAATCTCTTTTTACCCCAAATTCACAGTTTTGGAGAGATTTAGCAGAACTGCAGGGGACAACAGATGTACATGAGGGGCCTTGGACATGGAAATCAGGAGAGATGTCGTGTTTAACACAGGTAGTTACCTACATAAATGCTGCAACAAAGTTGGTTAAAGCTGTTAAAGCCACAGAGGAGCAAACATATCTCACAGCAAACGGAAGGGAATTTTCTGTATTGGCAAGTGTTTGTACACCCGAAGTTCCATATGGAAGTTCATTCAAGGTTGAATTGCTTTACAAGATATTGCCTGGACCAGAGCTACCTTCAGGGGAAGAATCTTCTCACCTTGTGGTATCCTGGGAAGTTAACTTTCTCCATAGCACATTGATGAAAGGCATGATTGAAGGAGGAGTTCGACAGGGACTTAAGGACAGTTTCGAGCAGTTCTCCATCTTGATGGCTCAGAAGTTTAAAGTGCTGGATTCTACAGATTTGTCTGACAAGGATCATATCCTGGCTAATATGCAGGCAGGACACCAATCAGATTGGGAATTGGCAAAGGAATACTTCTGGAATATGACTGTTGTTTCCACTATTTTTATGGTTTTATATATCATGGTGCACATTTTACTTTGTGAACCAAGTAAACTCCAAGGCCTAGAATTTTATGGACTTGATTTACCAGATAGTATCGGAGAGTTTATCACATGTGGAATTCTAGTCCTTCAATTGGAGCGCATTTATAATATGGTTCAACACTTTGTGCAAGCTAGGTTGCAACAAGGAAGTGATCATGGAGTCAAATCCCAAGGTGATGGATGGGTTCTCACTGTTGCCTTGATTGAAGGGACAAACCTAGCCTCCTTGGATTCATCAGGGTTCTCTGATCCCTATGTGGTTTTCACCAGCAATGGTAAAACAAGAACAAGCTCTGTCAGGCTTCAAACTACTGATCCTCAATGGAACGACATACTTGAGTTCGATGCTATGGAAGAACCTCCATCAGTTCTAGACGTGGAAGTTTTTGATTTTGATGGCCCTTTTGACCAAGCCATGTCTCTAGGGCATGCTGAGATCAATTTTCTGAAACACTCAGCTACTGAACTCGCAGACATGTGGGTCTCCCTTGAGGGAAAGCTTGCTCAGTCTTCTCAATCAAAGCTGCACTTGAGAATCTTTCTGGACAATAACAAAGGACATGAAACAATTAAGGAGTATATGACCAAAATGGAAAAGGAGGTTGGAAAGAAGTTGGATCTCCGATCGCCTCATAGGAATTCTACATTTCAGAAATTGTTTGGGTTGCCACCAGAAGAATTTCTCGTCAGTGATTTTACATGTGCACTGAAAAGGAAAATGCCAGTGCAGGGCCGACTTTTTCTATCTGCAAGAATAGTCGGGTTTTATGCAAACTTGTTTGGACACAAAACAAAATTTTTCTTTCTTTGGGAGGATATTGAAGATATCCAAGAGCATCCACCCTCCCTATCATCTGTGGGAAGCCCTTTGCTGGTCATTGTTTTGAAAAAGGATCGAGGTATTGATGCAAGGCATGGGGCGAAGTGCCAAGATGAAGAAGGCCGGCTTAGATTTTATTTCCAATCATTCTTGTCGTTCAGTTCAGCAAGCAGGATGATAGTGGGCTTGTGGAGAACAAGAGCACTAAGTCCAGATCAGAAAGCACAAATTGCTGAAGATCTGGATGATCATGAAGAAAGGTCCAAGATGCTTGAAGATACTGCATCCATACTCAATCTTGAAGATACAAAGATGTCTAAAGTTTATACTGCAGAAATACCTGTGAATACAAAATCTTTGATGGAAATGTTCAATGGAGGAAAGCTGGAGCACAAAATTATGGAGAAATCAGGTTGTCTTCACTATGCAACTACTGCATGGGAACCTATAAAGCCAAATATCTTTGAGCGACGTTTGTCTTATAGATTCAACCGCAATGTTTCCATCTTTGGGGGAGAGGTGGCATGCAGACAACGAAAGTCTCCAAATGCAAATGGTGAGGGATGGATCATCGATGAAGTTATGGCCCTGTACGGTGTTCCATTTGGCGATCATTTTCGGGTTCAGTTAAGGTACCGGATTGAGAAATCTGCCCTTGCTCATAATGCATGTAAATGCAATGTCTATGTCAGGGTCACCTGGCTCAAGAGCACGATGTTTCTGGAAAGGATCAAACACAACATAACTGGGAAGTTTGCTCATAGACTGAAGGATATATTTGAAATGGTAGAGAGAGAGATTCTCTTGACACCTCAACAGGACATTGGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1021

Amino Acids

115.76

Weight (kDa)

5.57

Isoelectric Point (pI)

49.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 102 1.8e-15 C2 domain
VASt PF16016 250 - 399 2.5e-29 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 533 - 634 9.3e-15 C2 domain
GRAM PF02893 686 - 776 1.1e-16 GRAM domain
VASt PF16016 852 - 991 1e-23 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50170
fragaria_vesca FvH4_5g06260 FvH4_5g06260 FvH4_5g06260
malus_domestica MD06G1166100.v1.1
prunus_persica Prupe.5G166000_v2.0.a1
pyrus_communis pycom06g14860
rosa_chinensis RchiOBHm_Chr7g0192601
rosa_laevigata RLG00000004348
rosa_multiflora Rmu_sc0004161.1_g000002
rosa_roxburghii Rroxscaffold_3G00262850
rosa_rugosa Rorug07G0002600 Rorug07G0002700 Rorug07G0002800
rosa_samantha Rh7AG127000 Rh7BG129600 Rh7CG131100 Rh7CG132000 Rh7DG130600
rosa_wichuraiana Rw7G010940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1518
AasI GACNNNNNNGTC 1 cut(s) 1892
Acc65I GGTACC 1 cut(s) 2865
AccB1I GGYRCC 1 cut(s) 2865
AciI CCGC 5 cut(s) 227, 293, 297, 399, 2725
AclWI GGATC 7 cut(s) 1287, 1655, 1721, 2037, 2313, 2807, 2969
AcsI RAATTY 9 cut(s) 357, 744, 808, 998, 1436, 1488, 2050, 2090, 2207
AcuI CTGAAG 4 cut(s) 53, 1083, 2487, 3020
AdeI CACNNNGTG 3 cut(s) 779, 1123, 1537
AfaI GTAC 5 cut(s) 286, 858, 1024, 2825, 2867
AfiI CCNNNNNNNGG 7 cut(s) 96, 326, 327, 1504, 2141, 2284, 2742
AflII CTTAAG 1 cut(s) 1199
AflIII ACRYGT 4 cut(s) 12, 1481, 1887, 2111
AjiI CACGTC 1 cut(s) 1789
AjnI CCWGG 5 cut(s) 192, 1081, 1130, 1287, 2930
AjuI GAANNNNNNNTTGG 2 cut(s) 1998, 2030
Alw21I GWGCWC 5 cut(s) 1398, 2119, 2437, 2625, 2945
Alw26I GTCTC 5 cut(s) 544, 687, 1836, 1900, 2778
Alw44I GTGCAC 2 cut(s) 1394, 2115
AlwI GGATC 7 cut(s) 1287, 1655, 1721, 2037, 2313, 2807, 2969
AlwNI CAGNNNCTG 2 cut(s) 629, 1874
AoxI GGCC 9 cut(s) 317, 388, 866, 1429, 1810, 2143, 2353, 2817, 3058
ApaLI GTGCAC 2 cut(s) 1394, 2115
ApeKI GCWGC 5 cut(s) 269, 376, 401, 929, 1936
ApoI RAATTY 9 cut(s) 357, 744, 808, 998, 1436, 1488, 2050, 2090, 2207
ArsI GACNNNNNNTTYG 6 cut(s) 41, 73, 85, 117, 449, 481
Asp700I GAANNNNTTC 1 cut(s) 2091
Asp718I GGTACC 1 cut(s) 2865
AspLEI GCGC 1 cut(s) 1512
AspS9I GGNCC 7 cut(s) 318, 866, 1085, 1811, 2143, 2493, 2818
AsuC2I CCSGG 4 cut(s) 242, 328, 519, 738
AsuHPI GGTGA 7 cut(s) 182, 765, 1109, 1598, 1669, 2801, 2920
AvaII GGWCC 2 cut(s) 1085, 2493
BaeGI GKGCMC 2 cut(s) 1398, 2119
BanI GGYRCC 1 cut(s) 2865
BbsI GAAGAC 2 cut(s) 1914, 2642
Bbv12I GWGCWC 5 cut(s) 1398, 2119, 2437, 2625, 2945
BbvI GCAGC 5 cut(s) 256, 363, 388, 916, 1923
BciT130I CCWGG 5 cut(s) 194, 1083, 1132, 1289, 2932
BciVI GTATCC 1 cut(s) 1138
BclI TGATCA 3 cut(s) 754, 1564, 2479
BcnI CCSGG 4 cut(s) 242, 328, 519, 738
BcoDI GTCTC 5 cut(s) 544, 687, 1836, 1900, 2778
BfaI CTAG 8 cut(s) 96, 618, 1433, 1493, 1545, 1631, 1784, 1835
BfmI CTRYAG 3 cut(s) 839, 1260, 2562
BfrI CTTAAG 1 cut(s) 1199
BfuI GTATCC 1 cut(s) 1138
BglII AGATCT 1 cut(s) 2470
BisI GCNGC 7 cut(s) 270, 293, 377, 399, 402, 930, 1937
BlsI GCNGC 7 cut(s) 271, 294, 378, 400, 403, 931, 1938
Bme1390I CCNGG 9 cut(s) 194, 242, 328, 519, 738, 1083, 1132, 1289, 2932
Bme18I GGWCC 2 cut(s) 1085, 2493
BmgBI CACGTC 1 cut(s) 1789
BmgT120I GGNCC 7 cut(s) 318, 866, 1085, 1811, 2143, 2493, 2818
BmiI GGNNCC 4 cut(s) 444, 867, 2676, 2867
BmrFI CCNGG 9 cut(s) 194, 242, 328, 519, 738, 1083, 1132, 1289, 2932
BmrI ACTGGG 1 cut(s) 2988
BmsI GCATC 7 cut(s) 385, 684, 1747, 2257, 2308, 2490, 2525
BmuI ACTGGG 1 cut(s) 2988
BoxI GACNNNNGTC 1 cut(s) 2707
BpiI GAAGAC 2 cut(s) 1914, 2642
BplI GAGNNNNNCTC 4 cut(s) 1205, 1237, 3018, 3050
BpmI CTGGAG 1 cut(s) 2639
BpuEI CTTGAG 4 cut(s) 1769, 1922, 1963, 2921
BpuMI CCSGG 4 cut(s) 242, 328, 519, 738
Bsa29I ATCGAT 1 cut(s) 2805
BsaAI YACGTR 1 cut(s) 13
BsaI GGTCTC 1 cut(s) 1900
BsaJI CCNNGG 6 cut(s) 737, 869, 1131, 1426, 1582, 1638
BsaWI WCCGGW 2 cut(s) 253, 2868
BsaXI ACNNNNNCTCC 2 cut(s) 1619, 1649
Bsc4I CCNNNNNNNGG 7 cut(s) 96, 326, 327, 1504, 2141, 2284, 2742
Bse118I RCCGGY 1 cut(s) 2355
Bse1I ACTGG 2 cut(s) 2135, 2983
Bse3DI GCAATG 3 cut(s) 1690, 2734, 2917
BseBI CCWGG 5 cut(s) 194, 1083, 1132, 1289, 2932
BseCI ATCGAT 1 cut(s) 2805
BseDI CCNNGG 6 cut(s) 737, 869, 1131, 1426, 1582, 1638
BseGI GGATG 8 cut(s) 202, 540, 1598, 2248, 2412, 2482, 2516, 2801
BseLI CCNNNNNNNGG 7 cut(s) 96, 326, 327, 1504, 2141, 2284, 2742
BseMI GCAATG 3 cut(s) 1690, 2734, 2917
BseMII CTCAG 7 cut(s) 264, 429, 621, 1250, 1836, 1881, 1931
BseNI ACTGG 2 cut(s) 2135, 2983
BseRI GAGGAG 4 cut(s) 182, 455, 980, 1197
BseSI GKGCMC 2 cut(s) 1398, 2119
BseXI GCAGC 5 cut(s) 256, 363, 388, 916, 1923
BsgI GTGCAG 2 cut(s) 1922, 2159
Bsh1285I CGRYCG 1 cut(s) 2039
BshFI GGCC 9 cut(s) 319, 390, 868, 1431, 1812, 2145, 2355, 2819, 3060
BshNI GGYRCC 1 cut(s) 2865
BshVI ATCGAT 1 cut(s) 2805
BsiEI CGRYCG 1 cut(s) 2039
BsiHKAI GWGCWC 5 cut(s) 1398, 2119, 2437, 2625, 2945
BsiSI CCGG 7 cut(s) 241, 254, 327, 518, 737, 2356, 2869
BslFI GGGAC 3 cut(s) 859, 1209, 1636
BslI CCNNNNNNNGG 7 cut(s) 96, 326, 327, 1504, 2141, 2284, 2742
BsmAI GTCTC 5 cut(s) 544, 687, 1836, 1900, 2778
BsmBI CGTCTC 1 cut(s) 544
BsmFI GGGAC 3 cut(s) 859, 1209, 1636
BsmI GAATGC 1 cut(s) 658
BsnI GGCC 9 cut(s) 319, 390, 868, 1431, 1812, 2145, 2355, 2819, 3060
Bso31I GGTCTC 1 cut(s) 1900
Bsp1286I GDGCHC 5 cut(s) 1398, 2119, 2437, 2625, 2945
Bsp1407I TGTACA 2 cut(s) 856, 1022
BspACI CCGC 5 cut(s) 227, 293, 297, 399, 2725
BspANI GGCC 9 cut(s) 319, 390, 868, 1431, 1812, 2145, 2355, 2819, 3060
BspCNI CTCAG 7 cut(s) 265, 430, 620, 1249, 1837, 1880, 1930
BspDI ATCGAT 1 cut(s) 2805
BspHI TCATGA 2 cut(s) 31, 2482
BspLI GGNNCC 4 cut(s) 444, 867, 2676, 2867
BspMAI CTGCAG 2 cut(s) 843, 2566
BspPI GGATC 7 cut(s) 1287, 1655, 1721, 2037, 2313, 2807, 2969
BspT107I GGYRCC 1 cut(s) 2865
BspTI CTTAAG 1 cut(s) 1199
BspTNI GGTCTC 1 cut(s) 1900
BsrDI GCAATG 3 cut(s) 1690, 2734, 2917
BsrFI RCCGGY 1 cut(s) 2355
BsrGI TGTACA 2 cut(s) 856, 1022
BsrI ACTGG 2 cut(s) 2135, 2983
BssAI RCCGGY 1 cut(s) 2355
BssECI CCNNGG 6 cut(s) 737, 869, 1131, 1426, 1582, 1638
BssT1I CCWWGG 4 cut(s) 869, 1426, 1582, 1638
Bst2UI CCWGG 5 cut(s) 194, 1083, 1132, 1289, 2932
Bst4CI ACNGT 6 cut(s) 461, 816, 1208, 1357, 1606, 2828
Bst6I CTCTTC 1 cut(s) 129
BstAFI CTTAAG 1 cut(s) 1199
BstAPI GCANNNNNTGC 2 cut(s) 2462, 2666
BstAUI TGTACA 2 cut(s) 856, 1022
BstBAI YACGTR 1 cut(s) 13
BstC8I GCNNGC 8 cut(s) 297, 1302, 1542, 1842, 1914, 2357, 2401, 2758
BstEII GGTNACC 1 cut(s) 2926
BstF5I GGATG 8 cut(s) 202, 540, 1598, 2248, 2412, 2482, 2516, 2801
BstHHI GCGC 1 cut(s) 1512
BstMAI GTCTC 5 cut(s) 544, 687, 1836, 1900, 2778
BstMCI CGRYCG 1 cut(s) 2039
BstMWI GCNNNNNNNGC 4 cut(s) 956, 2462, 2666, 2897
BstNI CCWGG 5 cut(s) 194, 1083, 1132, 1289, 2932
BstNSI RCATGY 6 cut(s) 1485, 1844, 1891, 2115, 2760, 2904
BstPAI GACNNNNGTC 1 cut(s) 2707
BstPI GGTNACC 1 cut(s) 2926
BstSCI CCNGG 9 cut(s) 192, 240, 326, 517, 736, 1081, 1130, 1287, 2930
BstSFI CTRYAG 3 cut(s) 839, 1260, 2562
BstSLI GKGCMC 2 cut(s) 1398, 2119
BstV1I GCAGC 5 cut(s) 256, 363, 388, 916, 1923
BstV2I GAAGAC 2 cut(s) 1914, 2642
BstX2I RGATCY 2 cut(s) 2029, 2470
BstXI CCANNNNNNTGG 2 cut(s) 1231, 1589
BstYI RGATCY 2 cut(s) 2029, 2470
Bsu15I ATCGAT 1 cut(s) 2805
BsuI GTATCC 1 cut(s) 1138
BsuRI GGCC 9 cut(s) 319, 390, 868, 1431, 1812, 2145, 2355, 2819, 3060
BsuTUI ATCGAT 1 cut(s) 2805
BtrI CACGTC 1 cut(s) 1789
BtsCI GGATG 8 cut(s) 202, 540, 1598, 2248, 2412, 2482, 2516, 2801
BtsIMutI CAGTG 4 cut(s) 1602, 2107, 2117, 2142
Cac8I GCNNGC 8 cut(s) 297, 1302, 1542, 1842, 1914, 2357, 2401, 2758
CaiI CAGNNNCTG 2 cut(s) 629, 1874
CciI TCATGA 2 cut(s) 31, 2482
CfoI GCGC 1 cut(s) 1512
Cfr10I RCCGGY 1 cut(s) 2355
Cfr13I GGNCC 7 cut(s) 318, 866, 1085, 1811, 2143, 2493, 2818
ClaI ATCGAT 1 cut(s) 2805
Csp6I GTAC 5 cut(s) 285, 857, 1023, 2824, 2866
CviQI GTAC 5 cut(s) 285, 857, 1023, 2824, 2866
DraI TTTAAA 1 cut(s) 1246
DraIII CACNNNGTG 3 cut(s) 779, 1123, 1537
DrdI GACNNNNNNGTC 1 cut(s) 1892
DseDI GACNNNNNNGTC 1 cut(s) 1892
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
EciI GGCGGA 1 cut(s) 242
Eco130I CCWWGG 4 cut(s) 869, 1426, 1582, 1638
Eco147I AGGCCT 1 cut(s) 1431
Eco31I GGTCTC 1 cut(s) 1900
Eco32I GATATC 1 cut(s) 2239
Eco47I GGWCC 2 cut(s) 1085, 2493
Eco57I CTGAAG 4 cut(s) 53, 1083, 2487, 3020
Eco91I GGTNACC 1 cut(s) 2926
EcoO109I RGGNCCY 1 cut(s) 866
EcoO65I GGTNACC 1 cut(s) 2926
EcoRI GAATTC 3 cut(s) 744, 1488, 2050
EcoRII CCWGG 5 cut(s) 192, 1081, 1130, 1287, 2930
EcoRV GATATC 1 cut(s) 2239
EcoT14I CCWWGG 4 cut(s) 869, 1426, 1582, 1638
EcoT22I ATGCAT 1 cut(s) 2902
ErhI CCWWGG 4 cut(s) 869, 1426, 1582, 1638
Esp3I CGTCTC 1 cut(s) 544
FalI AAGNNNNNCTT 6 cut(s) 1325, 1357, 1925, 1957, 2343, 2375
FaqI GGGAC 3 cut(s) 859, 1209, 1636
FauI CCCGC 1 cut(s) 304
FauNDI CATATG 1 cut(s) 1039
FbaI TGATCA 3 cut(s) 754, 1564, 2479
Fnu4HI GCNGC 7 cut(s) 270, 293, 377, 399, 402, 930, 1937
FokI GGATG 8 cut(s) 209, 547, 1605, 2235, 2419, 2489, 2503, 2808
Fsp4HI GCNGC 7 cut(s) 270, 293, 377, 399, 402, 930, 1937
FspBI CTAG 8 cut(s) 96, 618, 1433, 1493, 1545, 1631, 1784, 1835
GlaI GCGC 1 cut(s) 1511
GluI GCNGC 7 cut(s) 270, 293, 377, 399, 402, 930, 1937
GsuI CTGGAG 1 cut(s) 2639
HaeIII GGCC 9 cut(s) 319, 390, 868, 1431, 1812, 2145, 2355, 2819, 3060
HapII CCGG 7 cut(s) 241, 254, 327, 518, 737, 2356, 2869
HhaI GCGC 1 cut(s) 1512
Hin6I GCGC 1 cut(s) 1510
HinP1I GCGC 1 cut(s) 1510
HincII GTYRAC 1 cut(s) 1141
HindII GTYRAC 1 cut(s) 1141
HindIII AAGCTT 2 cut(s) 585, 1910
HpaI GTTAAC 1 cut(s) 1141
HpaII CCGG 7 cut(s) 241, 254, 327, 518, 737, 2356, 2869
HphI GGTGA 7 cut(s) 182, 765, 1109, 1598, 1669, 2801, 2920
Hpy166II GTNNAC 6 cut(s) 1025, 1141, 1396, 1412, 1421, 2117
Hpy8I GTNNAC 6 cut(s) 1025, 1141, 1396, 1412, 1421, 2117
Hpy99I CGWCG 1 cut(s) 2706
HpyAV CCTTC 9 cut(s) 40, 532, 987, 1107, 1172, 1508, 1613, 2345, 2995
HpyCH4III ACNGT 6 cut(s) 461, 816, 1208, 1357, 1606, 2828
HpyCH4IV ACGT 4 cut(s) 12, 553, 1788, 2704
HpyF10VI GCNNNNNNNGC 4 cut(s) 956, 2462, 2666, 2897
HpySE526I ACGT 4 cut(s) 12, 553, 1788, 2704
HspAI GCGC 1 cut(s) 1510
KpnI GGTACC 1 cut(s) 2869
KroI GCCGGC 1 cut(s) 2355
KroNI GCCGGC 1 cut(s) 2357
Ksp22I TGATCA 3 cut(s) 754, 1564, 2479
KspAI GTTAAC 1 cut(s) 1141
LmnI GCTCC 6 cut(s) 64, 169, 442, 967, 1507, 2620
Lsp1109I GCAGC 5 cut(s) 256, 363, 388, 916, 1923
LweI GCATC 7 cut(s) 385, 684, 1747, 2257, 2308, 2490, 2525
MaeI CTAG 8 cut(s) 96, 618, 1433, 1493, 1545, 1631, 1784, 1835
MaeII ACGT 4 cut(s) 12, 553, 1788, 2704
MaeIII GTNAC 2 cut(s) 913, 2926
MfeI CAATTG 1 cut(s) 1502
MflI RGATCY 2 cut(s) 2029, 2470
MhlI GDGCHC 5 cut(s) 1398, 2119, 2437, 2625, 2945
MlyI GAGTC 3 cut(s) 44, 197, 1581
MmeI TCCRAC 2 cut(s) 1996, 2007
Mph1103I ATGCAT 1 cut(s) 2902
MroNI GCCGGC 1 cut(s) 2355
MroXI GAANNNNTTC 1 cut(s) 2091
MslI CAYNNNNRTG 4 cut(s) 195, 1160, 1229, 2905
MspA1I CMGCKG 1 cut(s) 401
MspCI CTTAAG 1 cut(s) 1199
MspI CCGG 7 cut(s) 241, 254, 327, 518, 737, 2356, 2869
MspR9I CCNGG 9 cut(s) 194, 242, 328, 519, 738, 1083, 1132, 1289, 2932
MunI CAATTG 1 cut(s) 1502
Mva1269I GAATGC 1 cut(s) 658
MvaI CCWGG 5 cut(s) 194, 1083, 1132, 1289, 2932
MwoI GCNNNNNNNGC 4 cut(s) 956, 2462, 2666, 2897
NaeI GCCGGC 1 cut(s) 2357
NciI CCSGG 4 cut(s) 242, 328, 519, 738
NdeI CATATG 1 cut(s) 1039
NgoMIV GCCGGC 1 cut(s) 2355
NlaIV GGNNCC 4 cut(s) 444, 867, 2676, 2867
NmuCI GTSAC 1 cut(s) 2926
NsiI ATGCAT 1 cut(s) 2902
NspI RCATGY 6 cut(s) 1485, 1844, 1891, 2115, 2760, 2904
PaeI GCATGC 2 cut(s) 1844, 2760
PagI TCATGA 2 cut(s) 31, 2482
PceI AGGCCT 1 cut(s) 1431
PciI ACATGT 3 cut(s) 1481, 1887, 2111
PctI GAATGC 1 cut(s) 658
PdiI GCCGGC 1 cut(s) 2357
PdmI GAANNNNTTC 1 cut(s) 2091
PfoI TCCNGGA 1 cut(s) 326
PkrI GCNGC 7 cut(s) 271, 294, 378, 400, 403, 931, 1938
Ple19I CGATCG 1 cut(s) 2039
PleI GAGTC 3 cut(s) 44, 197, 1580
PpsI GAGTC 3 cut(s) 44, 197, 1580
Ppu21I YACGTR 1 cut(s) 13
PscI ACATGT 3 cut(s) 1481, 1887, 2111
PshAI GACNNNNGTC 1 cut(s) 2707
PsiI TTATAA 1 cut(s) 1518
Psp6I CCWGG 5 cut(s) 192, 1081, 1130, 1287, 2930
PspEI GGTNACC 1 cut(s) 2926
PspGI CCWGG 5 cut(s) 192, 1081, 1130, 1287, 2930
PspN4I GGNNCC 4 cut(s) 444, 867, 2676, 2867
PspPI GGNCC 7 cut(s) 318, 866, 1085, 1811, 2143, 2493, 2818
PstI CTGCAG 2 cut(s) 843, 2566
PstNI CAGNNNCTG 2 cut(s) 629, 1874
PsuI RGATCY 2 cut(s) 2029, 2470
PvuI CGATCG 1 cut(s) 2039
RsaI GTAC 5 cut(s) 286, 858, 1024, 2825, 2867
RsaNI GTAC 5 cut(s) 285, 857, 1023, 2824, 2866
RseI CAYNNNNRTG 4 cut(s) 195, 1160, 1229, 2905
SatI GCNGC 7 cut(s) 270, 293, 377, 399, 402, 930, 1937
Sau96I GGNCC 7 cut(s) 318, 866, 1085, 1811, 2143, 2493, 2818
SchI GAGTC 3 cut(s) 44, 197, 1581
ScrFI CCNGG 9 cut(s) 194, 242, 328, 519, 738, 1083, 1132, 1289, 2932
SduI GDGCHC 5 cut(s) 1398, 2119, 2437, 2625, 2945
SfaNI GCATC 7 cut(s) 385, 684, 1747, 2257, 2308, 2490, 2525
SfcI CTRYAG 3 cut(s) 839, 1260, 2562
SinI GGWCC 2 cut(s) 1085, 2493
SmiMI CAYNNNNRTG 4 cut(s) 195, 1160, 1229, 2905
SmlI CTYRAG 5 cut(s) 1199, 1748, 1901, 1942, 2936
SmoI CTYRAG 5 cut(s) 1199, 1748, 1901, 1942, 2936
SphI GCATGC 2 cut(s) 1844, 2760
SseBI AGGCCT 1 cut(s) 1431
SsiI CCGC 5 cut(s) 227, 293, 297, 399, 2725
SspMI CTAG 8 cut(s) 96, 618, 1433, 1493, 1545, 1631, 1784, 1835
StuI AGGCCT 1 cut(s) 1431
StyD4I CCNGG 9 cut(s) 192, 240, 326, 517, 736, 1081, 1130, 1287, 2930
StyI CCWWGG 4 cut(s) 869, 1426, 1582, 1638
TaaI ACNGT 6 cut(s) 461, 816, 1208, 1357, 1606, 2828
TaiI ACGT 4 cut(s) 15, 556, 1791, 2707
TaqI TCGA 5 cut(s) 1189, 1212, 1755, 2308, 2805
TatI WGTACW 2 cut(s) 856, 1022
TauI GCSGC 2 cut(s) 295, 401
TscAI CASTG 4 cut(s) 1609, 2107, 2124, 2142
TseFI GTSAC 1 cut(s) 2926
TseI GCWGC 5 cut(s) 269, 376, 401, 929, 1936
Tsp45I GTSAC 1 cut(s) 2926
TspGWI ACGGA 1 cut(s) 1005
TspRI CASTG 4 cut(s) 1609, 2107, 2124, 2142
Vha464I CTTAAG 1 cut(s) 1199
VneI GTGCAC 2 cut(s) 1394, 2115
VpaK11BI GGWCC 2 cut(s) 1085, 2493
XapI RAATTY 9 cut(s) 357, 744, 808, 998, 1436, 1488, 2050, 2090, 2207
XbaI TCTAGA 1 cut(s) 1783
XceI RCATGY 6 cut(s) 1485, 1844, 1891, 2115, 2760, 2904
XcmI CCANNNNNNNNNTGG 1 cut(s) 1372
XmnI GAANNNNTTC 1 cut(s) 2091
XspI CTAG 8 cut(s) 96, 618, 1433, 1493, 1545, 1631, 1784, 1835
Zsp2I ATGCAT 1 cut(s) 2902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.