Rorug07G0002800

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0011354
Physical Location & Seq
Reverse (-)
284337 .. 289048
4712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0002800.1

Sequence Viewer

Length: 1122 bp
ATGTCGTCCTCGGGCGGCAACTCCGGCATCGCCCCCTCTCAGCTGTACTTTTGCCACCAGTGCGATCGCACCGTTTCCATCAACCCTTCCCCTACCTCCGATCTCAATTGCCCTATTTGCACTGGCGGCTTCCTCGAAGAGATGGAAACCCCTAACCCTAACCCTAACCCTACCCCCAATCCCTTCTTCTCCTTCCCCTCCGACGCCCCTCCCTTCGCCTCCTCCAGCGGCTTTCCCTCCTTTCTCTTCAGCACCCCAGCAGGCCCCGCCGCCGGTGGTCCGGGAAATCTTGGCGACGAACTCTCCGCCCTCTTCGGTGGTGGCGCGCCTTCCTCCCGCTCGTCCCCCTTTCAGGACCCCGACGCCTTCGACCCCTTTGTGTTCCTCCAGAACTATTTGCAAACCCTAAGGGCCAATGGTGCCAATGTTCAGTTTGTGATTGATAACAGCGGCGGAGGAGGGGATCCTTCAGGCTTCCGTGTTCCCACCAATCTTAATTTTGGAGATTACTTCTTCGGCCCAGGCCTCGAGCAACTCATCCAGCAGCTTGCCGAGAATGATCCAAACCGCTACGGCACGCCACCGGCTTCAAAGTCTGCCATCGAGGCCTTGCCGGTTGTGAAAATCTCAAAGGAACTCTTGGATTCTGATTCTTCTCAGTGCGCAGTGTGTAAGGACTCGTTTGAGCTTGGGGAGGAGGCCAAGCAGATGCCCTGCAAGCACTTATACCATTCTGATTGTATTCTTCCCTGGTTGGAATTGCATAACTCATGCCCTGTTTGTCGCCACGAGTTGCCAACCGATGATTCAGAGTATGAGCAGAGGATTCGTGGCGGGGGCGATGCAGCCAATGCAAATACAACTCAATCTTCTGCTGCTGCTGCCAACAATTCCAGTGGTGTTGGTGGGAGTAGGGGGCCAGCTGTTGACATTCAGAGTCAGGCACCACGTACAGTTGAGCGGAGGTTCACCATTTCCCTGCCTCGCTTTTTAAGGGTATTTGGTGGTGCACCCGAGACTAGTAATACTGGGAGTGCAAGTGGAAACAATGATGGGCCTAACTCAGGGAATAGGGGAAGCCATAACTCGGAATCTGAGCCTAGACAAGAAGATCTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

373

Amino Acids

39.33

Weight (kDa)

4.57

Isoelectric Point (pI)

59.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 14 - 48 1.6e-14 zinc-ribbon
zf-RING_2 PF13639 220 - 262 4.7e-13 Ring finger domain
zf-C3HC4_2 PF13923 220 - 261 7.4e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 220 - 248 7.2e-08 RING-like zinc finger
zf-C3HC4 PF00097 221 - 261 2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50170
fragaria_vesca FvH4_5g06260 FvH4_5g06260 FvH4_5g06260
malus_domestica MD06G1166100.v1.1
prunus_persica Prupe.5G166000_v2.0.a1
pyrus_communis pycom06g14860
rosa_chinensis RchiOBHm_Chr7g0192601
rosa_laevigata RLG00000004348
rosa_multiflora Rmu_sc0004161.1_g000002
rosa_roxburghii Rroxscaffold_3G00262850
rosa_rugosa Rorug07G0002600 Rorug07G0002700 Rorug07G0002800
rosa_samantha Rh7AG127000 Rh7BG129600 Rh7CG131100 Rh7CG132000 Rh7DG130600
rosa_wichuraiana Rw7G010940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 664
AccB1I GGYRCC 2 cut(s) 419, 943
AccBSI CCGCTC 2 cut(s) 339, 961
AccII CGCG 1 cut(s) 326
AclWI GGATC 3 cut(s) 458, 471, 554
AcuI CTGAAG 2 cut(s) 232, 453
AcyI GRCGYC 2 cut(s) 204, 363
AfaI GTAC 2 cut(s) 47, 952
AfiI CCNNNNNNNGG 4 cut(s) 272, 352, 1064, 1087
AgsI TTSAA 1 cut(s) 591
AhlI ACTAGT 1 cut(s) 1019
AjnI CCWGG 2 cut(s) 520, 749
AjuI GAANNNNNNNTTGG 4 cut(s) 170, 202, 790, 822
AluBI AGCT 4 cut(s) 43, 547, 688, 923
AluI AGCT 4 cut(s) 43, 547, 688, 923
Alw21I GWGCWC 1 cut(s) 1012
Alw26I GTCTC 1 cut(s) 1010
Alw44I GTGCAC 1 cut(s) 1008
AlwI GGATC 3 cut(s) 458, 471, 554
Ama87I CYCGRG 3 cut(s) 10, 527, 1013
AoxI GGCC 8 cut(s) 262, 411, 517, 523, 606, 699, 917, 1055
ApaLI GTGCAC 1 cut(s) 1008
ApeKI GCWGC 5 cut(s) 544, 845, 875, 878, 881
AscI GGCGCGCC 1 cut(s) 324
AsiSI GCGATCGC 1 cut(s) 67
AspLEI GCGC 3 cut(s) 326, 328, 665
AspS9I GGNCC 7 cut(s) 263, 278, 355, 411, 518, 917, 1055
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 1 cut(s) 961
AvaI CYCGRG 3 cut(s) 10, 527, 1013
AvaII GGWCC 2 cut(s) 278, 355
AxyI CCTNAGG 1 cut(s) 407
BaeGI GKGCMC 1 cut(s) 1012
BamHI GGATCC 1 cut(s) 463
BanI GGYRCC 2 cut(s) 419, 943
BauI CACGAG 1 cut(s) 788
Bbv12I GWGCWC 1 cut(s) 1012
BbvI GCAGC 5 cut(s) 556, 857, 862, 865, 868
BccI CCATC 4 cut(s) 86, 136, 608, 1046
BceAI ACGGC 1 cut(s) 589
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 2 cut(s) 522, 751
BcnI CCSGG 1 cut(s) 282
BcoDI GTCTC 1 cut(s) 1010
BcuI ACTAGT 1 cut(s) 1019
BfaI CTAG 2 cut(s) 1020, 1101
BglI GCCNNNNNGGC 1 cut(s) 605
BglII AGATCT 1 cut(s) 1111
Bme1390I CCNGG 3 cut(s) 282, 522, 751
Bme18I GGWCC 2 cut(s) 278, 355
BmeT110I CYCGRG 3 cut(s) 10, 527, 1013
BmgT120I GGNCC 7 cut(s) 263, 278, 355, 411, 518, 917, 1055
BmiI GGNNCC 6 cut(s) 265, 357, 421, 465, 918, 945
BmrFI CCNGG 3 cut(s) 282, 522, 751
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 3 cut(s) 36, 699, 832
BmuI ACTGGG 1 cut(s) 1038
BpmI CTGGAG 2 cut(s) 208, 371
BpuMI CCSGG 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 950
BsaHI GRCGYC 2 cut(s) 204, 363
BsaJI CCNNGG 3 cut(s) 9, 520, 749
BsaXI ACNNNNNCTCC 4 cut(s) 287, 317, 1024, 1054
Bsc4I CCNNNNNNNGG 4 cut(s) 272, 352, 1064, 1087
Bse118I RCCGGY 3 cut(s) 272, 583, 613
Bse1I ACTGG 4 cut(s) 58, 127, 894, 1033
Bse21I CCTNAGG 1 cut(s) 407
BseBI CCWGG 2 cut(s) 522, 751
BseDI CCNNGG 3 cut(s) 9, 520, 749
BseGI GGATG 1 cut(s) 537
BseLI CCNNNNNNNGG 4 cut(s) 272, 352, 1064, 1087
BseMII CTCAG 4 cut(s) 53, 671, 1077, 1086
BseNI ACTGG 4 cut(s) 58, 127, 894, 1033
BsePI GCGCGC 1 cut(s) 324
BseRI GAGGAG 3 cut(s) 211, 471, 710
BseSI GKGCMC 1 cut(s) 1012
BseXI GCAGC 5 cut(s) 556, 857, 862, 865, 868
BseYI CCCAGC 1 cut(s) 256
Bsh1236I CGCG 1 cut(s) 326
Bsh1285I CGRYCG 1 cut(s) 67
BshFI GGCC 8 cut(s) 264, 413, 519, 525, 608, 701, 919, 1057
BshNI GGYRCC 2 cut(s) 419, 943
BsiEI CGRYCG 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 1012
BsiHKCI CYCGRG 3 cut(s) 10, 527, 1013
BsiSI CCGG 5 cut(s) 24, 273, 281, 584, 614
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 4 cut(s) 272, 352, 1064, 1087
BsmAI GTCTC 1 cut(s) 1010
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 8 cut(s) 264, 413, 519, 525, 608, 701, 919, 1057
BsoBI CYCGRG 3 cut(s) 10, 527, 1013
Bsp1286I GDGCHC 1 cut(s) 1012
Bsp143I GATC 5 cut(s) 64, 100, 463, 559, 1111
BspANI GGCC 8 cut(s) 264, 413, 519, 525, 608, 701, 919, 1057
BspCNI CTCAG 4 cut(s) 52, 670, 1076, 1087
BspFNI CGCG 1 cut(s) 326
BspLI GGNNCC 6 cut(s) 265, 357, 421, 465, 918, 945
BspPI GGATC 3 cut(s) 458, 471, 554
BspT107I GGYRCC 2 cut(s) 419, 943
BsrBI CCGCTC 2 cut(s) 339, 961
BsrFI RCCGGY 3 cut(s) 272, 583, 613
BsrI ACTGG 4 cut(s) 58, 127, 894, 1033
BssAI RCCGGY 3 cut(s) 272, 583, 613
BssECI CCNNGG 3 cut(s) 9, 520, 749
BssHII GCGCGC 1 cut(s) 324
BssMI GATC 5 cut(s) 64, 100, 463, 559, 1111
BssNI GRCGYC 2 cut(s) 204, 363
BssSI CACGAG 1 cut(s) 788
Bst2BI CACGAG 1 cut(s) 788
Bst2UI CCWGG 2 cut(s) 522, 751
Bst4CI ACNGT 2 cut(s) 73, 955
Bst6I CTCTTC 3 cut(s) 132, 251, 317
BstACI GRCGYC 2 cut(s) 204, 363
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBAI YACGTR 1 cut(s) 950
BstC8I GCNNGC 6 cut(s) 262, 326, 549, 578, 719, 921
BstDEI CTNAG 5 cut(s) 39, 407, 657, 1063, 1095
BstENI CCTNNNNNAGG 1 cut(s) 1062
BstF5I GGATG 1 cut(s) 537
BstFNI CGCG 1 cut(s) 326
BstHHI GCGC 3 cut(s) 326, 328, 665
BstKTI GATC 5 cut(s) 67, 103, 466, 562, 1114
BstMAI GTCTC 1 cut(s) 1010
BstMBI GATC 5 cut(s) 64, 100, 463, 559, 1111
BstMCI CGRYCG 1 cut(s) 67
BstNI CCWGG 2 cut(s) 522, 751
BstSCI CCNGG 3 cut(s) 280, 520, 749
BstSLI GKGCMC 1 cut(s) 1012
BstUI CGCG 1 cut(s) 326
BstV1I GCAGC 5 cut(s) 556, 857, 862, 865, 868
BstX2I RGATCY 2 cut(s) 463, 1111
BstYI RGATCY 2 cut(s) 463, 1111
Bsu36I CCTNAGG 1 cut(s) 407
BsuRI GGCC 8 cut(s) 264, 413, 519, 525, 608, 701, 919, 1057
BtgZI GCGATG 2 cut(s) 13, 855
BtsCI GGATG 1 cut(s) 537
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 5 cut(s) 65, 120, 665, 672, 901
Cac8I GCNNGC 6 cut(s) 262, 326, 549, 578, 719, 921
CfoI GCGC 3 cut(s) 326, 328, 665
Cfr10I RCCGGY 3 cut(s) 272, 583, 613
Cfr13I GGNCC 7 cut(s) 263, 278, 355, 411, 518, 917, 1055
CseI GACGC 2 cut(s) 212, 371
Csp6I GTAC 2 cut(s) 46, 951
CspCI CAANNNNNGTGG 2 cut(s) 877, 912
CviAII CATG 1 cut(s) 771
CviQI GTAC 2 cut(s) 46, 951
DdeI CTNAG 5 cut(s) 39, 407, 657, 1063, 1095
DpnI GATC 5 cut(s) 66, 102, 465, 561, 1113
DpnII GATC 5 cut(s) 64, 100, 463, 559, 1111
Eam1104I CTCTTC 3 cut(s) 132, 251, 317
EarI CTCTTC 3 cut(s) 132, 251, 317
EciI GGCGGA 2 cut(s) 295, 468
Eco147I AGGCCT 2 cut(s) 525, 608
Eco47I GGWCC 2 cut(s) 278, 355
Eco57I CTGAAG 2 cut(s) 232, 453
Eco81I CCTNAGG 1 cut(s) 407
Eco88I CYCGRG 3 cut(s) 10, 527, 1013
EcoNI CCTNNNNNAGG 1 cut(s) 1062
EcoO109I RGGNCCY 2 cut(s) 263, 355
EcoRII CCWGG 2 cut(s) 520, 749
FaeI CATG 1 cut(s) 774
FaiI YATR 5 cut(s) 727, 765, 772, 816, 1083
FalI AAGNNNNNCTT 3 cut(s) 623, 655, 1098
FaqI GGGAC 1 cut(s) 328
FatI CATG 1 cut(s) 770
FauI CCCGC 3 cut(s) 274, 344, 827
FokI GGATG 1 cut(s) 524
FspBI CTAG 2 cut(s) 1020, 1101
FspI TGCGCA 1 cut(s) 664
GlaI GCGC 3 cut(s) 325, 327, 664
GsaI CCCAGC 1 cut(s) 260
GsuI CTGGAG 2 cut(s) 208, 371
HaeIII GGCC 8 cut(s) 264, 413, 519, 525, 608, 701, 919, 1057
HapII CCGG 5 cut(s) 24, 273, 281, 584, 614
HgaI GACGC 2 cut(s) 212, 371
HhaI GCGC 3 cut(s) 326, 328, 665
Hin1I GRCGYC 2 cut(s) 204, 363
Hin1II CATG 1 cut(s) 774
Hin6I GCGC 3 cut(s) 324, 326, 663
HinP1I GCGC 3 cut(s) 324, 326, 663
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HinfI GANTC 7 cut(s) 644, 650, 677, 806, 826, 937, 1091
HpaII CCGG 5 cut(s) 24, 273, 281, 584, 614
HphI GGTGA 1 cut(s) 961
Hpy166II GTNNAC 3 cut(s) 928, 969, 1010
Hpy188I TCNGA 8 cut(s) 100, 202, 649, 736, 811, 936, 1090, 1096
Hpy188III TCNNGA 3 cut(s) 353, 388, 1115
Hpy8I GTNNAC 3 cut(s) 928, 969, 1010
Hpy99I CGWCG 3 cut(s) 206, 299, 365
HpyAV CCTTC 7 cut(s) 96, 193, 202, 223, 339, 376, 477
HpyCH4III ACNGT 2 cut(s) 73, 955
HpyCH4IV ACGT 1 cut(s) 949
HpyCH4V TGCA 8 cut(s) 120, 400, 717, 763, 845, 854, 1010, 1037
HpyF3I CTNAG 5 cut(s) 39, 407, 657, 1063, 1095
HpySE526I ACGT 1 cut(s) 949
Hsp92I GRCGYC 2 cut(s) 204, 363
Hsp92II CATG 1 cut(s) 774
HspAI GCGC 3 cut(s) 324, 326, 663
Kzo9I GATC 5 cut(s) 64, 100, 463, 559, 1111
Lsp1109I GCAGC 5 cut(s) 556, 857, 862, 865, 868
LweI GCATC 3 cut(s) 36, 699, 832
MaeI CTAG 2 cut(s) 1020, 1101
MaeII ACGT 1 cut(s) 949
MalI GATC 5 cut(s) 66, 102, 465, 561, 1113
MbiI CCGCTC 2 cut(s) 339, 961
MboI GATC 5 cut(s) 64, 100, 463, 559, 1111
MboII GAAGA 9 cut(s) 149, 178, 238, 304, 505, 645, 737, 861, 1121
MfeI CAATTG 1 cut(s) 106
MflI RGATCY 2 cut(s) 463, 1111
MhlI GDGCHC 1 cut(s) 1012
MluCI AATT 4 cut(s) 106, 496, 758, 889
MlyI GAGTC 2 cut(s) 671, 946
MmeI TCCRAC 2 cut(s) 225, 735
MseI TTAA 2 cut(s) 495, 992
MspA1I CMGCKG 4 cut(s) 43, 228, 450, 923
MspI CCGG 5 cut(s) 24, 273, 281, 584, 614
MspR9I CCNGG 3 cut(s) 282, 522, 751
MunI CAATTG 1 cut(s) 106
MvaI CCWGG 2 cut(s) 522, 751
MvnI CGCG 1 cut(s) 326
NciI CCSGG 1 cut(s) 282
NdeII GATC 5 cut(s) 64, 100, 463, 559, 1111
NlaIII CATG 1 cut(s) 774
NlaIV GGNNCC 6 cut(s) 265, 357, 421, 465, 918, 945
NmeAIII GCCGAG 1 cut(s) 577
NsbI TGCGCA 1 cut(s) 664
PaeR7I CTCGAG 1 cut(s) 527
PalAI GGCGCGCC 1 cut(s) 324
PauI GCGCGC 1 cut(s) 324
PceI AGGCCT 2 cut(s) 525, 608
PfeI GAWTC 5 cut(s) 644, 650, 806, 826, 1091
PfoI TCCNGGA 1 cut(s) 280
Ple19I CGATCG 1 cut(s) 67
PleI GAGTC 2 cut(s) 671, 945
PpsI GAGTC 2 cut(s) 671, 945
Ppu21I YACGTR 1 cut(s) 950
PpuMI RGGWCCY 1 cut(s) 355
Psp5II RGGWCCY 1 cut(s) 355
Psp6I CCWGG 2 cut(s) 520, 749
PspFI CCCAGC 1 cut(s) 256
PspGI CCWGG 2 cut(s) 520, 749
PspN4I GGNNCC 6 cut(s) 265, 357, 421, 465, 918, 945
PspPI GGNCC 7 cut(s) 263, 278, 355, 411, 518, 917, 1055
PspPPI RGGWCCY 1 cut(s) 355
PspXI VCTCGAGB 1 cut(s) 527
PsuI RGATCY 2 cut(s) 463, 1111
PteI GCGCGC 1 cut(s) 324
PvuI CGATCG 1 cut(s) 67
PvuII CAGCTG 2 cut(s) 43, 923
RgaI GCGATCGC 1 cut(s) 67
RsaI GTAC 2 cut(s) 47, 952
RsaNI GTAC 2 cut(s) 46, 951
SaqAI TTAA 2 cut(s) 495, 992
Sau3AI GATC 5 cut(s) 64, 100, 463, 559, 1111
Sau96I GGNCC 7 cut(s) 263, 278, 355, 411, 518, 917, 1055
SchI GAGTC 2 cut(s) 671, 946
ScrFI CCNGG 3 cut(s) 282, 522, 751
SduI GDGCHC 1 cut(s) 1012
SetI ASST 7 cut(s) 45, 98, 549, 690, 925, 952, 968
SfaAI GCGATCGC 1 cut(s) 67
SfaNI GCATC 3 cut(s) 36, 699, 832
Sfr274I CTCGAG 1 cut(s) 527
SgfI GCGATCGC 1 cut(s) 67
SgrAI CRCCGGYG 1 cut(s) 272
SgsI GGCGCGCC 1 cut(s) 324
SinI GGWCC 2 cut(s) 278, 355
SlaI CTCGAG 1 cut(s) 527
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
SpeI ACTAGT 1 cut(s) 1019
Sse9I AATT 4 cut(s) 106, 496, 758, 889
SseBI AGGCCT 2 cut(s) 525, 608
SspMI CTAG 2 cut(s) 1020, 1101
StuI AGGCCT 2 cut(s) 525, 608
StyD4I CCNGG 3 cut(s) 280, 520, 749
TaaI ACNGT 2 cut(s) 73, 955
TaiI ACGT 1 cut(s) 952
TaqI TCGA 4 cut(s) 135, 369, 528, 603
TasI AATT 4 cut(s) 106, 496, 758, 889
TatI WGTACW 1 cut(s) 45
TauI GCSGC 5 cut(s) 18, 129, 231, 272, 453
TfiI GAWTC 5 cut(s) 644, 650, 806, 826, 1091
Tru1I TTAA 2 cut(s) 495, 992
Tru9I TTAA 2 cut(s) 495, 992
TscAI CASTG 5 cut(s) 65, 127, 665, 672, 901
TseI GCWGC 5 cut(s) 544, 845, 875, 878, 881
TspGWI ACGGA 1 cut(s) 467
TspRI CASTG 5 cut(s) 65, 127, 665, 672, 901
VneI GTGCAC 1 cut(s) 1008
VpaK11BI GGWCC 2 cut(s) 278, 355
XagI CCTNNNNNAGG 1 cut(s) 1062
XhoI CTCGAG 1 cut(s) 527
XspI CTAG 2 cut(s) 1020, 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.