RLG00000004348

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011354
Physical Location & Seq
Forward (+)
58601382 .. 58606648
5267 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004348

Sequence Viewer

Length: 3048 bp
ATGAGGCTTTACGTGTATGTGATGGAAGCTGATGACTTGGTTGTGAAGGACTCTTGCTTTGTGGAGCTTCAGGTTGGGAAGCACAAGTCCAAGACTAGGATTTTGAGGAACACCAACAACCCTGCTTGGAATGAAGAGTTTGTGTTCAGGGTGTATGATATGAATGAGGAGTTACTTGTGTCTGTTTTTCACCAGGATGATGACTCTAATGGTCTGTTTAATGGGGCGGAGTTAGTCGGCCGGGTTCGGATTCCGGTGGGGTCTGTTGCTGCTGAGAATAATAGTACCTTGCCCCCCGCTTGGTATTCTTTTGAAAGGCCCAAGTCCGGGAAGTTCATCAATACAGACTGTGGAAAAATTCTTCTCACTCTATCGTTGCATGAAAAAGGCCATTATGCTGCTGCTAATGACATCCCTTGTTTGCACTCAAATATAACTGAGGATCCTAAAGAGATTGACAGTCCATTTGTATCATCTAATGGTGTCTTATGCTCCAAATCTCCATGGGGAAAGATACCGGGAGGAAAACAATTGATGAAGGCTCTTAGGAGACGTATGGACAGGCTTCTTCATAAGAATGATGAAGCTTCAAGAACTGACTCCTCAGAGCTGTCTAGTTCATTATCTGACTATGAAGATTGCATGGAGGAACAGCATTCTAATTGTAGCTTTGACGAGTCTCTTGAAATGATGCAGTCAAGTGAGAGCAAACAAAATATGCCAGAGAATCTTCCGGGGGGGGTTCTTATTGATCAGGCTTATGTTGTTTCACCAAATGATCTTAATGCATCACTTTTTACCCCAAATTCACAGTTTAGGAGAGATTTAGCAGAACTGCAGGGGACAACAAATGTACATGAGGGGCCTTGGACATGGAAATCAGGAGAGATGTCGTGTTTAACACGGGTAGTTACCTACATAAATGCTGCAACAAAGTTGGTTAAAGCTGTTGAAGCCACAGAGGAGCAAACATATCTCACAGCAAACGGAAGGGAATTTGCTGTATGGGCAAGTGTTTGTACACCCGAAGTTCCATATGGAAGTTCATTTAAGGTTGAATTGCTTTACAAGATATTGCCTGGACCAGAGCTACCTTCAGGGGAAGAATCTTCTCGCCTTGTAGTATCCTGGGAAGTTAACTTTCTCCATAGCACATTGATGAAAGGCATGATTGAAGGAGGAGTTCGACAGGGACTTAAGGACAGTTTCGAGCAGTTCTCCATCTTGATGGCTCAGAAGTTTAAAGTGCTGGATTCTACAGATTTGTCTGACAAGGATCAGATCCTGGCTAATATGCAGGCAGGACACCAATCAGATTGGGAATTGGCAAAGGAATACTTCTGGAATATGACCGTTGTTTCCACTATTTTTATGGTTTTATATATCATGGTGCACATTTTACTTTGTGAACCAAGTAAACTCCAAGGCCTAGAATTTCATGGACTTGATTTGCCAGATAGTATCGGAGAGTTTATCACATGTGGAATTCTAGTCCTTCAATTGGAGCGCATTTATAATATGGTTCAACACTTTGTGCAAGCTAGGTTGCAACAAGGAAGTGATCATGGAGTCAAATCCCAAGGTGATGGATGGGTTCTCACTGTTGCCTTGATTGAAGGGACAAACCTAGCCACCTTGGATTCATCAGGGTTCTCTGATCCCTATGTGGTTTTCACCAGCAATGGTAAAACAAGAACAAGCTCTGTCAGGCTTCAAACTACTGATCCTCAATGGAACGACATACTTGAGTTCGATGCTATGGAAGAACCTCCATCAGTTCTAGATGTGGAAGTTTTTGATTTTGATGGCCCTTTTGACCAAGCTATGTCACTAGGGCATGCTGAGATCAATTTCCTGAAACACTCGGCAACTGAACTCGCAGACATGTGGGTCTCCCTTGAGGGAAAGCTTGCTCAGTCTTCTCAATCAAAGCTGCACTTAAGAATCTTTTTGGACAATAACAAAGGACTTGAAACAATAAAGGAGTATATGACCAAAATGGAAAAGGAGGTTGGAAAGAAGTTGGATCTCCGATCGCCTCATAGGAATTCTACATTTCAGAAATTGTTTGGGCTACCACCAGAAGAATTTCTCGTCAGTGATTTTACATGTGCACTGAAAAGGAAAATGCCAGTGCAGGGCCGACTTTTTCTATCTGCCAGAATAGTAGGGTTTTATGCAAACTTGTTTGGACACAAAACAAAATTTTTCTTTCTTTGGGAGGATATTGAAGATATCCAAGAGCATCCTCCCTCCCTATCATCTGTGGGAAGCCCTTTGCTGGTCATTGTTTTGAAAAAGGGTCGAGGTATTGATGCAAGGCATGGGGCGAAGTGCCAAGATGAAGAAGGCCGGCTTAGATTTTATTTCCAATCGTTCTTGACATTCAGTTCAGCAAGCAGGATGATAGTGGGCTTGTGGAGAACAAGAGCACTAAGTCCAGATCAGAAAGCACAAATTGCTGAAGATCTGGATGATCAGGAAGAAAGGTCCAAGATGCTTGAAGATACTGAATCCATACTCAATCTTGAAGATTCAAAGATGTCTGAAGTTTATACTACAGAAATACCTGTGAATACAAAATCTTTGATGGAAATGTTCAATGGAGGAAAGCTGGAGAACAAAATTATGGGGAAATCAGGTTGTCTTAACTATGCGACTACTGCATGGGAACCTATAAAGCCAAATGTCTTTGAGCGACGTTTGTCTTATAGATTCAACCGCAATGTTTCCATCTTTGGAGGAGAGGTGGCATGCAGACAGCGAAAGTCTCCAATTGGAAATGGTGAGGGATGGACCATTGATGAAGTTATGGCCTTGTACGGTGTTCCGTTTGGCGATCATTTTCGGGTTCAGTTAAGGTACCGGATTGAGGAATCTGCCCTTGCTCATAATGCATGTAAATGCAATGTCTATGTCAGGGTCACCTGGCTCAAGAGCACAATGTTTCTGGAAAGGATCAAACACAACATAACTGAGAAGTTTGCTCATAGACTGAAGGATATATTTGAAATGGTAGAGAGAGAGATTCTCTTGACACCTCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1016

Amino Acids

115.0

Weight (kDa)

5.42

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 102 3.3e-17 C2 domain
VASt PF16016 249 - 398 1.4e-28 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 532 - 633 9.8e-15 C2 domain
GRAM PF02893 685 - 767 3.8e-16 GRAM domain
VASt PF16016 851 - 992 1.8e-22 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50170
fragaria_vesca FvH4_5g06260 FvH4_5g06260 FvH4_5g06260
malus_domestica MD06G1166100.v1.1
prunus_persica Prupe.5G166000_v2.0.a1
pyrus_communis pycom06g14860
rosa_chinensis RchiOBHm_Chr7g0192601
rosa_laevigata RLG00000004348
rosa_multiflora Rmu_sc0004161.1_g000002
rosa_roxburghii Rroxscaffold_3G00262850
rosa_rugosa Rorug07G0002600 Rorug07G0002700 Rorug07G0002800
rosa_samantha Rh7AG127000 Rh7BG129600 Rh7CG131100 Rh7CG132000 Rh7DG130600
rosa_wichuraiana Rw7G010940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1515
AasI GACNNNNNNGTC 1 cut(s) 1889
Acc65I GGTACC 1 cut(s) 2862
AccB1I GGYRCC 1 cut(s) 2862
AciI CCGC 3 cut(s) 227, 297, 2722
AclWI GGATC 8 cut(s) 437, 450, 1276, 1284, 1652, 1718, 2034, 2966
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 8 cut(s) 357, 805, 995, 1433, 1485, 2047, 2087, 2204
AcuI CTGAAG 5 cut(s) 53, 1080, 2484, 2568, 3017
AdeI CACNNNGTG 1 cut(s) 1534
AfaI GTAC 5 cut(s) 286, 855, 1021, 2822, 2864
AfiI CCNNNNNNNGG 8 cut(s) 96, 300, 326, 327, 1501, 2138, 2281, 2872
AflII CTTAAG 2 cut(s) 1196, 1939
AflIII ACRYGT 4 cut(s) 12, 1478, 1884, 2108
AjnI CCWGG 5 cut(s) 192, 1078, 1127, 1284, 2927
AjuI GAANNNNNNNTTGG 2 cut(s) 1995, 2027
Alw21I GWGCWC 4 cut(s) 1395, 2116, 2434, 2942
Alw26I GTCTC 4 cut(s) 544, 684, 1897, 2775
Alw44I GTGCAC 2 cut(s) 1391, 2112
AlwI GGATC 8 cut(s) 437, 450, 1276, 1284, 1652, 1718, 2034, 2966
AlwNI CAGNNNCTG 1 cut(s) 1285
AoxI GGCC 9 cut(s) 238, 317, 388, 863, 1426, 1807, 2140, 2350, 2814
ApaLI GTGCAC 2 cut(s) 1391, 2112
ApeKI GCWGC 5 cut(s) 269, 398, 401, 926, 1933
ApoI RAATTY 8 cut(s) 357, 805, 995, 1433, 1485, 2047, 2087, 2204
ArsI GACNNNNNNTTYG 6 cut(s) 41, 73, 85, 117, 449, 481
Asp700I GAANNNNTTC 1 cut(s) 2088
Asp718I GGTACC 1 cut(s) 2862
AspLEI GCGC 1 cut(s) 1509
AspS9I GGNCC 7 cut(s) 318, 863, 1082, 1808, 2140, 2490, 2796
AsuC2I CCSGG 4 cut(s) 242, 328, 519, 735
AsuHPI GGTGA 6 cut(s) 182, 762, 1595, 1666, 2798, 2917
AvaII GGWCC 3 cut(s) 1082, 2490, 2796
BaeGI GKGCMC 2 cut(s) 1395, 2116
BamHI GGATCC 1 cut(s) 442
BanI GGYRCC 1 cut(s) 2862
BbsI GAAGAC 1 cut(s) 1911
Bbv12I GWGCWC 4 cut(s) 1395, 2116, 2434, 2942
BbvI GCAGC 5 cut(s) 256, 385, 388, 913, 1920
BciT130I CCWGG 5 cut(s) 194, 1080, 1129, 1286, 2929
BciVI GTATCC 1 cut(s) 1135
BclI TGATCA 3 cut(s) 751, 1561, 2476
BcnI CCSGG 4 cut(s) 242, 328, 519, 735
BcoDI GTCTC 4 cut(s) 544, 684, 1897, 2775
BfaI CTAG 8 cut(s) 96, 615, 1430, 1490, 1542, 1628, 1781, 1832
BfmI CTRYAG 3 cut(s) 836, 1257, 2559
BfrI CTTAAG 2 cut(s) 1196, 1939
BfuI GTATCC 1 cut(s) 1135
BglII AGATCT 1 cut(s) 2467
BisI GCNGC 5 cut(s) 270, 399, 402, 927, 1934
BlsI GCNGC 5 cut(s) 271, 400, 403, 928, 1935
Bme1390I CCNGG 9 cut(s) 194, 242, 328, 519, 735, 1080, 1129, 1286, 2929
Bme18I GGWCC 3 cut(s) 1082, 2490, 2796
BmgT120I GGNCC 7 cut(s) 318, 863, 1082, 1808, 2140, 2490, 2796
BmiI GGNNCC 4 cut(s) 444, 864, 2673, 2864
BmrFI CCNGG 9 cut(s) 194, 242, 328, 519, 735, 1080, 1129, 1286, 2929
BmsI GCATC 6 cut(s) 681, 797, 1744, 2254, 2305, 2487
BoxI GACNNNNGTC 1 cut(s) 2704
BpiI GAAGAC 1 cut(s) 1911
BplI GAGNNNNNCTC 4 cut(s) 1202, 1234, 3015, 3047
BpmI CTGGAG 1 cut(s) 2636
BpuEI CTTGAG 3 cut(s) 1766, 1919, 2918
BpuMI CCSGG 4 cut(s) 242, 328, 519, 735
BsaAI YACGTR 1 cut(s) 13
BsaI GGTCTC 1 cut(s) 1897
BsaJI CCNNGG 7 cut(s) 503, 734, 866, 1128, 1423, 1579, 1635
BsaWI WCCGGW 2 cut(s) 253, 2865
BsaXI ACNNNNNCTCC 2 cut(s) 2733, 2763
Bsc4I CCNNNNNNNGG 8 cut(s) 96, 300, 326, 327, 1501, 2138, 2281, 2872
Bse118I RCCGGY 1 cut(s) 2352
Bse1I ACTGG 1 cut(s) 2132
Bse3DI GCAATG 3 cut(s) 1687, 2731, 2914
BseBI CCWGG 5 cut(s) 194, 1080, 1129, 1286, 2929
BseDI CCNNGG 7 cut(s) 503, 734, 866, 1128, 1423, 1579, 1635
BseGI GGATG 7 cut(s) 202, 411, 1595, 2245, 2409, 2479, 2798
BseLI CCNNNNNNNGG 8 cut(s) 96, 300, 326, 327, 1501, 2138, 2281, 2872
BseMI GCAATG 3 cut(s) 1687, 2731, 2914
BseMII CTCAG 7 cut(s) 264, 429, 618, 1247, 1833, 1928, 2967
BseNI ACTGG 1 cut(s) 2132
BseRI GAGGAG 5 cut(s) 182, 592, 977, 1194, 2757
BseSI GKGCMC 2 cut(s) 1395, 2116
BseX3I CGGCCG 1 cut(s) 238
BseXI GCAGC 5 cut(s) 256, 385, 388, 913, 1920
BsgI GTGCAG 2 cut(s) 1919, 2156
Bsh1285I CGRYCG 2 cut(s) 241, 2036
BshFI GGCC 9 cut(s) 240, 319, 390, 865, 1428, 1809, 2142, 2352, 2816
BshNI GGYRCC 1 cut(s) 2862
BsiEI CGRYCG 2 cut(s) 241, 2036
BsiHKAI GWGCWC 4 cut(s) 1395, 2116, 2434, 2942
BsiSI CCGG 7 cut(s) 241, 254, 327, 518, 734, 2353, 2866
BslFI GGGAC 3 cut(s) 856, 1206, 1633
BslI CCNNNNNNNGG 8 cut(s) 96, 300, 326, 327, 1501, 2138, 2281, 2872
BsmAI GTCTC 4 cut(s) 544, 684, 1897, 2775
BsmBI CGTCTC 1 cut(s) 544
BsmFI GGGAC 3 cut(s) 856, 1206, 1633
BsmI GAATGC 1 cut(s) 655
BsnI GGCC 9 cut(s) 240, 319, 390, 865, 1428, 1809, 2142, 2352, 2816
Bso31I GGTCTC 1 cut(s) 1897
Bsp1286I GDGCHC 4 cut(s) 1395, 2116, 2434, 2942
Bsp1407I TGTACA 2 cut(s) 853, 1019
Bsp19I CCATGG 1 cut(s) 503
BspACI CCGC 3 cut(s) 227, 297, 2722
BspANI GGCC 9 cut(s) 240, 319, 390, 865, 1428, 1809, 2142, 2352, 2816
BspCNI CTCAG 7 cut(s) 265, 430, 617, 1246, 1834, 1927, 2968
BspLI GGNNCC 4 cut(s) 444, 864, 2673, 2864
BspMAI CTGCAG 1 cut(s) 840
BspPI GGATC 8 cut(s) 437, 450, 1276, 1284, 1652, 1718, 2034, 2966
BspT107I GGYRCC 1 cut(s) 2862
BspTI CTTAAG 2 cut(s) 1196, 1939
BspTNI GGTCTC 1 cut(s) 1897
BsrDI GCAATG 3 cut(s) 1687, 2731, 2914
BsrFI RCCGGY 1 cut(s) 2352
BsrGI TGTACA 2 cut(s) 853, 1019
BsrI ACTGG 1 cut(s) 2132
BssAI RCCGGY 1 cut(s) 2352
BssECI CCNNGG 7 cut(s) 503, 734, 866, 1128, 1423, 1579, 1635
BssT1I CCWWGG 5 cut(s) 503, 866, 1423, 1579, 1635
Bst2UI CCWGG 5 cut(s) 194, 1080, 1129, 1286, 2929
Bst4CI ACNGT 7 cut(s) 350, 461, 813, 1205, 1354, 1603, 2825
Bst6I CTCTTC 1 cut(s) 129
BstAFI CTTAAG 2 cut(s) 1196, 1939
BstAPI GCANNNNNTGC 1 cut(s) 2459
BstAUI TGTACA 2 cut(s) 853, 1019
BstBAI YACGTR 1 cut(s) 13
BstC8I GCNNGC 7 cut(s) 1299, 1539, 1839, 1911, 2354, 2398, 2755
BstDSI CCRYGG 1 cut(s) 503
BstEII GGTNACC 1 cut(s) 2923
BstF5I GGATG 7 cut(s) 202, 411, 1595, 2245, 2409, 2479, 2798
BstHHI GCGC 1 cut(s) 1509
BstMAI GTCTC 4 cut(s) 544, 684, 1897, 2775
BstMCI CGRYCG 2 cut(s) 241, 2036
BstMWI GCNNNNNNNGC 5 cut(s) 953, 1007, 2459, 2663, 2894
BstNI CCWGG 5 cut(s) 194, 1080, 1129, 1286, 2929
BstNSI RCATGY 6 cut(s) 1482, 1841, 1888, 2112, 2757, 2901
BstPAI GACNNNNGTC 1 cut(s) 2704
BstPI GGTNACC 1 cut(s) 2923
BstSCI CCNGG 9 cut(s) 192, 240, 326, 517, 733, 1078, 1127, 1284, 2927
BstSFI CTRYAG 3 cut(s) 836, 1257, 2559
BstSLI GKGCMC 2 cut(s) 1395, 2116
BstV1I GCAGC 5 cut(s) 256, 385, 388, 913, 1920
BstV2I GAAGAC 1 cut(s) 1911
BstX2I RGATCY 4 cut(s) 442, 1281, 2026, 2467
BstXI CCANNNNNNTGG 2 cut(s) 1228, 1586
BstYI RGATCY 4 cut(s) 442, 1281, 2026, 2467
BstZI CGGCCG 1 cut(s) 238
BsuI GTATCC 1 cut(s) 1135
BsuRI GGCC 9 cut(s) 240, 319, 390, 865, 1428, 1809, 2142, 2352, 2816
BtgI CCRYGG 1 cut(s) 503
BtsCI GGATG 7 cut(s) 202, 411, 1595, 2245, 2409, 2479, 2798
BtsIMutI CAGTG 4 cut(s) 1599, 2104, 2114, 2139
Cac8I GCNNGC 7 cut(s) 1299, 1539, 1839, 1911, 2354, 2398, 2755
CaiI CAGNNNCTG 1 cut(s) 1285
CfoI GCGC 1 cut(s) 1509
Cfr10I RCCGGY 1 cut(s) 2352
Cfr13I GGNCC 7 cut(s) 318, 863, 1082, 1808, 2140, 2490, 2796
Csp6I GTAC 5 cut(s) 285, 854, 1020, 2821, 2863
CviQI GTAC 5 cut(s) 285, 854, 1020, 2821, 2863
DraI TTTAAA 1 cut(s) 1243
DraIII CACNNNGTG 1 cut(s) 1534
DrdI GACNNNNNNGTC 1 cut(s) 1889
DseDI GACNNNNNNGTC 1 cut(s) 1889
EaeI YGGCCR 1 cut(s) 238
EagI CGGCCG 1 cut(s) 238
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
EciI GGCGGA 1 cut(s) 242
EclXI CGGCCG 1 cut(s) 238
Eco130I CCWWGG 5 cut(s) 503, 866, 1423, 1579, 1635
Eco147I AGGCCT 1 cut(s) 1428
Eco31I GGTCTC 1 cut(s) 1897
Eco32I GATATC 1 cut(s) 2236
Eco47I GGWCC 3 cut(s) 1082, 2490, 2796
Eco52I CGGCCG 1 cut(s) 238
Eco57I CTGAAG 5 cut(s) 53, 1080, 2484, 2568, 3017
Eco91I GGTNACC 1 cut(s) 2923
EcoO109I RGGNCCY 1 cut(s) 863
EcoO65I GGTNACC 1 cut(s) 2923
EcoRI GAATTC 2 cut(s) 1485, 2047
EcoRII CCWGG 5 cut(s) 192, 1078, 1127, 1284, 2927
EcoRV GATATC 1 cut(s) 2236
EcoT14I CCWWGG 5 cut(s) 503, 866, 1423, 1579, 1635
EcoT22I ATGCAT 2 cut(s) 790, 2899
ErhI CCWWGG 5 cut(s) 503, 866, 1423, 1579, 1635
Esp3I CGTCTC 1 cut(s) 544
FalI AAGNNNNNCTT 6 cut(s) 1322, 1354, 1922, 1954, 2340, 2372
FaqI GGGAC 3 cut(s) 856, 1206, 1633
FauI CCCGC 1 cut(s) 304
FauNDI CATATG 1 cut(s) 1036
FbaI TGATCA 3 cut(s) 751, 1561, 2476
Fnu4HI GCNGC 5 cut(s) 270, 399, 402, 927, 1934
FokI GGATG 7 cut(s) 209, 398, 1602, 2232, 2416, 2486, 2805
Fsp4HI GCNGC 5 cut(s) 270, 399, 402, 927, 1934
FspBI CTAG 8 cut(s) 96, 615, 1430, 1490, 1542, 1628, 1781, 1832
GlaI GCGC 1 cut(s) 1508
GluI GCNGC 5 cut(s) 270, 399, 402, 927, 1934
GsuI CTGGAG 1 cut(s) 2636
HaeIII GGCC 9 cut(s) 240, 319, 390, 865, 1428, 1809, 2142, 2352, 2816
HapII CCGG 7 cut(s) 241, 254, 327, 518, 734, 2353, 2866
HhaI GCGC 1 cut(s) 1509
Hin6I GCGC 1 cut(s) 1507
HinP1I GCGC 1 cut(s) 1507
HincII GTYRAC 1 cut(s) 1138
HindII GTYRAC 1 cut(s) 1138
HindIII AAGCTT 2 cut(s) 585, 1907
HpaI GTTAAC 1 cut(s) 1138
HpaII CCGG 7 cut(s) 241, 254, 327, 518, 734, 2353, 2866
HphI GGTGA 6 cut(s) 182, 762, 1595, 1666, 2798, 2917
Hpy166II GTNNAC 6 cut(s) 1022, 1138, 1393, 1409, 1418, 2114
Hpy8I GTNNAC 6 cut(s) 1022, 1138, 1393, 1409, 1418, 2114
Hpy99I CGWCG 1 cut(s) 2703
HpyAV CCTTC 9 cut(s) 40, 532, 984, 1104, 1169, 1505, 1610, 2342, 2992
HpyCH4III ACNGT 7 cut(s) 350, 461, 813, 1205, 1354, 1603, 2825
HpyCH4IV ACGT 3 cut(s) 12, 553, 2701
HpyF10VI GCNNNNNNNGC 5 cut(s) 953, 1007, 2459, 2663, 2894
HpySE526I ACGT 3 cut(s) 12, 553, 2701
HspAI GCGC 1 cut(s) 1507
KpnI GGTACC 1 cut(s) 2866
KroI GCCGGC 1 cut(s) 2352
KroNI GCCGGC 1 cut(s) 2354
Ksp22I TGATCA 3 cut(s) 751, 1561, 2476
KspAI GTTAAC 1 cut(s) 1138
LmnI GCTCC 4 cut(s) 64, 497, 964, 1504
Lsp1109I GCAGC 5 cut(s) 256, 385, 388, 913, 1920
LweI GCATC 6 cut(s) 681, 797, 1744, 2254, 2305, 2487
MaeI CTAG 8 cut(s) 96, 615, 1430, 1490, 1542, 1628, 1781, 1832
MaeII ACGT 3 cut(s) 12, 553, 2701
MaeIII GTNAC 4 cut(s) 171, 910, 1827, 2923
MfeI CAATTG 3 cut(s) 530, 1499, 2775
MflI RGATCY 4 cut(s) 442, 1281, 2026, 2467
MhlI GDGCHC 4 cut(s) 1395, 2116, 2434, 2942
MlyI GAGTC 5 cut(s) 44, 197, 593, 686, 1578
MmeI TCCRAC 2 cut(s) 1993, 2004
Mph1103I ATGCAT 2 cut(s) 790, 2899
MroNI GCCGGC 1 cut(s) 2352
MroXI GAANNNNTTC 1 cut(s) 2088
MslI CAYNNNNRTG 5 cut(s) 195, 576, 1157, 1226, 2902
MspCI CTTAAG 2 cut(s) 1196, 1939
MspI CCGG 7 cut(s) 241, 254, 327, 518, 734, 2353, 2866
MspR9I CCNGG 9 cut(s) 194, 242, 328, 519, 735, 1080, 1129, 1286, 2929
MunI CAATTG 3 cut(s) 530, 1499, 2775
Mva1269I GAATGC 1 cut(s) 655
MvaI CCWGG 5 cut(s) 194, 1080, 1129, 1286, 2929
MwoI GCNNNNNNNGC 5 cut(s) 953, 1007, 2459, 2663, 2894
NaeI GCCGGC 1 cut(s) 2354
NciI CCSGG 4 cut(s) 242, 328, 519, 735
NcoI CCATGG 1 cut(s) 503
NdeI CATATG 1 cut(s) 1036
NgoMIV GCCGGC 1 cut(s) 2352
NlaIV GGNNCC 4 cut(s) 444, 864, 2673, 2864
NmeAIII GCCGAG 1 cut(s) 1844
NmuCI GTSAC 2 cut(s) 1827, 2923
NsiI ATGCAT 2 cut(s) 790, 2899
NspI RCATGY 6 cut(s) 1482, 1841, 1888, 2112, 2757, 2901
PaeI GCATGC 2 cut(s) 1841, 2757
PceI AGGCCT 1 cut(s) 1428
PciI ACATGT 3 cut(s) 1478, 1884, 2108
PctI GAATGC 1 cut(s) 655
PdiI GCCGGC 1 cut(s) 2354
PdmI GAANNNNTTC 1 cut(s) 2088
PfoI TCCNGGA 1 cut(s) 326
PkrI GCNGC 5 cut(s) 271, 400, 403, 928, 1935
Ple19I CGATCG 1 cut(s) 2036
PleI GAGTC 5 cut(s) 44, 197, 593, 685, 1577
PpsI GAGTC 5 cut(s) 44, 197, 593, 685, 1577
Ppu21I YACGTR 1 cut(s) 13
PscI ACATGT 3 cut(s) 1478, 1884, 2108
PshAI GACNNNNGTC 1 cut(s) 2704
PsiI TTATAA 1 cut(s) 1515
Psp6I CCWGG 5 cut(s) 192, 1078, 1127, 1284, 2927
PspEI GGTNACC 1 cut(s) 2923
PspGI CCWGG 5 cut(s) 192, 1078, 1127, 1284, 2927
PspN4I GGNNCC 4 cut(s) 444, 864, 2673, 2864
PspPI GGNCC 7 cut(s) 318, 863, 1082, 1808, 2140, 2490, 2796
PstI CTGCAG 1 cut(s) 840
PstNI CAGNNNCTG 1 cut(s) 1285
PsuI RGATCY 4 cut(s) 442, 1281, 2026, 2467
PvuI CGATCG 1 cut(s) 2036
RsaI GTAC 5 cut(s) 286, 855, 1021, 2822, 2864
RsaNI GTAC 5 cut(s) 285, 854, 1020, 2821, 2863
RseI CAYNNNNRTG 5 cut(s) 195, 576, 1157, 1226, 2902
SatI GCNGC 5 cut(s) 270, 399, 402, 927, 1934
Sau96I GGNCC 7 cut(s) 318, 863, 1082, 1808, 2140, 2490, 2796
SchI GAGTC 5 cut(s) 44, 197, 593, 686, 1578
ScrFI CCNGG 9 cut(s) 194, 242, 328, 519, 735, 1080, 1129, 1286, 2929
SduI GDGCHC 4 cut(s) 1395, 2116, 2434, 2942
SfaNI GCATC 6 cut(s) 681, 797, 1744, 2254, 2305, 2487
SfcI CTRYAG 3 cut(s) 836, 1257, 2559
SinI GGWCC 3 cut(s) 1082, 2490, 2796
SmiMI CAYNNNNRTG 5 cut(s) 195, 576, 1157, 1226, 2902
SmlI CTYRAG 5 cut(s) 1196, 1745, 1898, 1939, 2933
SmoI CTYRAG 5 cut(s) 1196, 1745, 1898, 1939, 2933
SphI GCATGC 2 cut(s) 1841, 2757
SseBI AGGCCT 1 cut(s) 1428
SsiI CCGC 3 cut(s) 227, 297, 2722
SspMI CTAG 8 cut(s) 96, 615, 1430, 1490, 1542, 1628, 1781, 1832
StuI AGGCCT 1 cut(s) 1428
StyD4I CCNGG 9 cut(s) 192, 240, 326, 517, 733, 1078, 1127, 1284, 2927
StyI CCWWGG 5 cut(s) 503, 866, 1423, 1579, 1635
TaaI ACNGT 7 cut(s) 350, 461, 813, 1205, 1354, 1603, 2825
TaiI ACGT 3 cut(s) 15, 556, 2704
TaqI TCGA 4 cut(s) 1186, 1209, 1752, 2305
TatI WGTACW 2 cut(s) 853, 1019
TscAI CASTG 4 cut(s) 1606, 2104, 2121, 2139
TseFI GTSAC 2 cut(s) 1827, 2923
TseI GCWGC 5 cut(s) 269, 398, 401, 926, 1933
Tsp45I GTSAC 2 cut(s) 1827, 2923
TspGWI ACGGA 2 cut(s) 1002, 2820
TspRI CASTG 4 cut(s) 1606, 2104, 2121, 2139
Vha464I CTTAAG 2 cut(s) 1196, 1939
VneI GTGCAC 2 cut(s) 1391, 2112
VpaK11BI GGWCC 3 cut(s) 1082, 2490, 2796
XapI RAATTY 8 cut(s) 357, 805, 995, 1433, 1485, 2047, 2087, 2204
XbaI TCTAGA 1 cut(s) 1780
XceI RCATGY 6 cut(s) 1482, 1841, 1888, 2112, 2757, 2901
XcmI CCANNNNNNNNNTGG 1 cut(s) 1369
XmnI GAANNNNTTC 1 cut(s) 2088
XspI CTAG 8 cut(s) 96, 615, 1430, 1490, 1542, 1628, 1781, 1832
Zsp2I ATGCAT 2 cut(s) 790, 2899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.