MD06G1166100.v1.1

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011354
Physical Location & Seq
Reverse (-)
30597030 .. 30602327
5298 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1166100.v1.1.491

Sequence Viewer

Length: 3072 bp
ATGGATACTCAGGAATTGCTAACACAGCTGTTGAGTAGTGTTCTGAGTCGTATCTACGTGAAACTCCAGGTGCGGAGGCACAAGTCCAAGACCCGGGTTTTGAGAAGTACCACCAACCCGGTTTGGAATGAGGAGTTTGTTTTCGGGGTGCATGATGTGAATGAGGAGCTTGTGGTGTCTGTGTGTCACCAGGATGAGGAGCAGTCTGGCTTGTTCTATGGCGGGTCTGAGTTGGTGGGTCGGGTTTCGATTCCGGTTTCGTCTGTTGCTGCTGAGAGTACTTGCACCTTGCCTCCGGCTTGGTATGCTCTTGAAAGTCCCAGGACTGGGAAGTTCATTAGCAAAGATTGTGGGAAAATTCTTCTCACCCTTTCTCTGCATGAAAAAGGCCATGATGCTTCCATTGATTACCTTCTCAGTGATCATTCAAATATAAGCCTTGAGGAACCTAATGAGGTGGACAGTCCATTTATGTCAACTCACAGTGTCTTTTCTTCCAAATCTTTATGTTCCAAGATGCCAGGTGGTATACAGTTGTTGAAGGCTATTGTGAGACGTATGGACAAGCTTATGCATAAGAACGATGAAGCGTCAAAAACAGGTGATTCTTCGGAGTTGTCTACTCCAGTATCTGATTATGAAGATTGTGTGGAGGAGCATCAAAGTAGTTGTAGCTTTAAAGAGTCTCTTGAGATGATGCGGTCAAGAGGAAGCGAACAAAAACTGCCGGAGAATCTGCTGGGAGGGATTCTTATTGATCAGGCATATGCTGTTTCACAACATGATCTTAATGCATCTCTTTTTACCCCAAATTCACAGTTTAGGACAGATCTTGCAGAACTGCAGGGGACAACAGATCTACAGGAGGGGCCTTGGACGTGGAAATCCGGAGAGATGCCATGTTTGACACGAGTTGTTTCCTACACAAAAGCTGCCTCAAAGTTAGTTAAGGCTGCTAAGGCCACAGAGGAGCAAACGTATATCACAGCAGATGGAAAGGAATTTGCTGTATTAATCAGTGCGAGTACACCTGATGTTCCATATGGAAATTCATTCAAGGTTGAGTTGCTTTACAAGATAATGTCTGGACCAGAGCTACCTTCAGCGGAACAATCCTCCCGTCTGGTGGTATCCTGGGACATTAACTTTCTCCAGAACACAATGATGAAAGGCATGATTGAACGAGGAGTTCGACAGGGACTTGAGGAGAGTTTTGGTCAGTTTTCCAGCTTGCTGGCTCGGAATTTCAAAACGCTGGATTCAACAGACTTGTCTGACAAGGATCATATCCTGGCAAGTACGAAGGCAGGGCAACAATCAGATTTGAAGTTGGCAACTGAATACTTCTGGAATTTCACTCTAGTTACCACTATCTTTATGGTTCTGTATGTCCTGGTTCATATTCTACTTTGTGAGCCCAGTACACCCCAGGGCTTGGAATTTATCGGACTTGATTTGCCACACAGTTTTGGAGAGCTCATCACATGTGGAATTCTCATCCTTCAGTTGGAGCGCGTTTATAACATGGTTTTACACTTTGTACAAGCTAGGTTGCAGAAAGGAAGTGATAATGGAGTCAAAGCACAAGGTGATGGATGGGTTCTTACTGTTGCTTTGATTGAAGGGAACAGCTTAGCATCCTTGCATTCATCAGGGTTCTCTGATCCCTATGTGGTTTTCACCTGCAATGGTAAAACAAGAGCAAGCTCTGTCAAGCTTCAAACAAGTGATCCTCAATGGAACGATATACTTGAGTTTGATGCTATGGAAGAACCGCCATCAGTTCTGGATGTTGAAGTTTTTGATTTTGATGGCCCATTTGACCGAGCTACATCACTTGGACATGCTGAGATCAATTTTCTTAAACACTCAGCTACCGAACTTGCAGATTTGTGGGTCTCCCTTGACGGAAAGCTTGCCCAGTCTTCTCAATCAAAGTTGCACGTCAGAATCTTTTTGGACAAGAACAAAGGAGCTGAAACAATTAGGGAGTATATGACCAAAATGGAGAGGGAAGTTGGAATGAAGTTGAATCTGCGATCGCCTCACAGGAATTCAACATTTCAGAAATTGTTTGGGTTGCCACCAGAAGAATTTCTTATCAGTGATTTTACATGCTCCCTGAGGAGGAAAATGCCTTTACAGGGCCGACTCTTTCTATCTGCAAGGATAGTTGGGTTTTACTCAAGCTTGTTTGGACACAAAACAAAATTTTTCTTTCTTTGGGAGGATATTGAAGATATCCAAGTTCTTCCCCCATCCTTATCATCTGTGGGAAGCCCCTTGCTGGCCATTGTTCTAAAAAAGGATCGAGGAATTGATGCAAGGCATGGTGCAAGGTGTCAAGACGATGAAGGCAGGCTGAAATTTCATTTCCAATCATTTTTATCGTCTAATTCAGCCAGCAGGACAATAATGGGCTTGTGGAGAACGAGAACACTTAGTGCTGACCAGAAAACACAAATTGCTGAAGAACATTTTGATCAAGAAGACATCCCCATAATGTTTGAAGACACTGAATCCATATTCAATCTTGAGGATGCAAAGATGTCCAAAGTTTATACTGCAGAACTACCTATTAACATAAAATCTCTGATGGAAATTTTCGAGGGAGGAAAGTTGGAGCATAAAATCATGGGGAAATCATGCTGTCTTAACTATGTAACTACCGCATGGGAACCTACAAAAAAGCCGGATGTCTTTGAACGACATCTATCTTACAGGTTCAACCGCCAGGTCTCAATCTTTGGAGGAGAGGTTACATGCAGACAAGAAAAGTCCCCCTTGGCAAACGGTGAAGGATGGATCGTGGATGAAGTTATGGCCCTGCAAGGCGTTCCATTTGAGGATCACTTTCGTGTTCAGCTCAGGTTCCGCATCGAGAAATCTGCCATGGCTCATAATGCATGTAAATGCAATGTTCATGTCAGGGTCTTCTGGCTTAAAAGGACAAAGTTTCAGGACAGGGTCACACGCAACATCGTAGGGAAATTCGCCCATCGACTCAAGGAAATATTCGAATTCGTCAAGCAGGAGATTCTCTTGGCACCGGGACCAGACGTTTCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1024

Amino Acids

115.21

Weight (kDa)

5.83

Isoelectric Point (pI)

49.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 15 - 92 2e-15 C2 domain
VASt PF16016 251 - 400 2.6e-32 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 534 - 635 3.7e-14 C2 domain
GRAM PF02893 687 - 785 1e-17 GRAM domain
VASt PF16016 853 - 994 1.8e-21 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50170
fragaria_vesca FvH4_5g06260 FvH4_5g06260 FvH4_5g06260
malus_domestica MD06G1166100.v1.1
prunus_persica Prupe.5G166000_v2.0.a1
pyrus_communis pycom06g14860
rosa_chinensis RchiOBHm_Chr7g0192601
rosa_laevigata RLG00000004348
rosa_multiflora Rmu_sc0004161.1_g000002
rosa_roxburghii Rroxscaffold_3G00262850
rosa_rugosa Rorug07G0002600 Rorug07G0002700 Rorug07G0002800
rosa_samantha Rh7AG127000 Rh7BG129600 Rh7CG131100 Rh7CG132000 Rh7DG130600
rosa_wichuraiana Rw7G010940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1521
AarI CACCTGC 1 cut(s) 1691
Acc36I ACCTGC 1 cut(s) 1691
AccB1I GGYRCC 1 cut(s) 3046
AccB7I CCANNNNNTGG 1 cut(s) 1435
AccI GTMKAC 2 cut(s) 529, 620
AccII CGCG 1 cut(s) 1515
AccIII TCCGGA 1 cut(s) 887
AciI CCGC 8 cut(s) 73, 222, 700, 1106, 1775, 2670, 2731, 2875
AclWI GGATC 6 cut(s) 1290, 1658, 1724, 2316, 2813, 2856
AcoI YGGCCR 1 cut(s) 2289
AcuI CTGAAG 3 cut(s) 1086, 1487, 2490
AdeI CACNNNGTG 2 cut(s) 1589, 2444
AfaI GTAC 6 cut(s) 109, 280, 1027, 1300, 1423, 1542
AflIII ACRYGT 1 cut(s) 1484
AjiI CACGTC 2 cut(s) 879, 1945
AjnI CCWGG 9 cut(s) 66, 189, 320, 520, 1133, 1290, 1392, 1428, 2733
AleI CACNNNNGTG 1 cut(s) 2856
Alw21I GWGCWC 1 cut(s) 1479
Alw26I GTCTC 4 cut(s) 547, 690, 1903, 2743
AlwI GGATC 6 cut(s) 1290, 1658, 1724, 2316, 2813, 2856
AlwNI CAGNNNCTG 1 cut(s) 632
Ama87I CYCGRG 1 cut(s) 93
Aor13HI TCCGGA 1 cut(s) 887
AoxI GGCC 7 cut(s) 388, 869, 960, 1813, 2146, 2289, 2823
ApeKI GCWGC 3 cut(s) 269, 932, 953
ArsI GACNNNNNNTTYG 2 cut(s) 82, 114
AseI ATTAAT 1 cut(s) 1013
AsiSI GCGATCGC 1 cut(s) 2042
Asp700I GAANNNNTTC 1 cut(s) 2094
AspLEI GCGC 1 cut(s) 1515
AspS9I GGNCC 6 cut(s) 869, 1088, 1814, 2146, 2824, 3053
AsuC2I CCSGG 4 cut(s) 94, 95, 119, 3051
AsuHPI GGTGA 6 cut(s) 179, 358, 614, 1601, 1672, 2807
AsuII TTCGAA 1 cut(s) 3018
AvaI CYCGRG 1 cut(s) 93
AvaII GGWCC 2 cut(s) 1088, 3053
AxyI CCTNAGG 1 cut(s) 2123
BaeI ACNNNNGTAYC 2 cut(s) 612, 645
BalI TGGCCA 1 cut(s) 2291
BanI GGYRCC 1 cut(s) 3046
BanII GRGCYC 2 cut(s) 1419, 1479
BauI CACGAG 1 cut(s) 909
BbsI GAAGAC 4 cut(s) 1917, 2496, 2517, 2926
Bbv12I GWGCWC 1 cut(s) 1479
BbvI GCAGC 3 cut(s) 256, 919, 940
BccI CCATC 9 cut(s) 986, 1586, 1590, 1786, 1805, 2266, 2590, 2796, 3006
BcgI CGANNNNNNTGC 2 cut(s) 2870, 2904
BciT130I CCWGG 9 cut(s) 68, 191, 322, 522, 1135, 1292, 1394, 1430, 2735
BciVI GTATCC 1 cut(s) 1141
BclI TGATCA 3 cut(s) 421, 757, 2482
BcnI CCSGG 4 cut(s) 94, 95, 119, 3051
BcoDI GTCTC 4 cut(s) 547, 690, 1903, 2743
BfaI CTAG 2 cut(s) 1361, 1548
BfmI CTRYAG 3 cut(s) 842, 860, 2565
BfuAI ACCTGC 1 cut(s) 1691
BfuI GTATCC 1 cut(s) 1141
BglII AGATCT 2 cut(s) 829, 856
BisI GCNGC 3 cut(s) 270, 933, 954
BlpI GCTNAGC 1 cut(s) 1633
BlsI GCNGC 3 cut(s) 271, 934, 955
BmcAI AGTACT 1 cut(s) 280
Bme18I GGWCC 2 cut(s) 1088, 3053
BmeT110I CYCGRG 1 cut(s) 93
BmgBI CACGTC 2 cut(s) 879, 1945
BmgT120I GGNCC 6 cut(s) 869, 1088, 1814, 2146, 2824, 3053
BmiI GGNNCC 6 cut(s) 447, 870, 2679, 2873, 3048, 3054
BmrI ACTGGG 3 cut(s) 336, 1413, 1915
BmuI ACTGGG 3 cut(s) 336, 1413, 1915
BpiI GAAGAC 4 cut(s) 1917, 2496, 2517, 2926
BpmI CTGGAG 3 cut(s) 50, 609, 1136
Bpu10I CCTNAGC 2 cut(s) 957, 2867
Bpu1102I GCTNAGC 1 cut(s) 1633
Bpu14I TTCGAA 1 cut(s) 3018
BpuEI CTTGAG 7 cut(s) 461, 710, 1223, 1772, 2170, 2555, 2990
BpuMI CCSGG 4 cut(s) 94, 95, 119, 3051
BsaAI YACGTR 1 cut(s) 58
BsaI GGTCTC 2 cut(s) 1903, 2743
BsaJI CCNNGG 8 cut(s) 93, 320, 872, 1134, 1428, 1429, 2784, 2892
BsaWI WCCGGW 2 cut(s) 253, 887
BsaXI ACNNNNNCTCC 6 cut(s) 277, 307, 962, 992, 2742, 2772
Bse1I ACTGG 4 cut(s) 331, 626, 1419, 1921
Bse21I CCTNAGG 1 cut(s) 2123
Bse3DI GCAATG 2 cut(s) 1693, 2923
BseAI TCCGGA 1 cut(s) 887
BseBI CCWGG 9 cut(s) 68, 191, 322, 522, 1135, 1292, 1394, 1430, 2735
BseDI CCNNGG 8 cut(s) 93, 320, 872, 1134, 1428, 1429, 2784, 2892
BseMI GCAATG 2 cut(s) 1693, 2923
BseMII CTCAG 9 cut(s) 23, 35, 219, 264, 430, 1839, 1884, 2114, 2881
BseNI ACTGG 4 cut(s) 331, 626, 1419, 1921
BseRI GAGGAG 9 cut(s) 146, 179, 212, 668, 983, 1200, 1220, 2140, 2766
BseXI GCAGC 3 cut(s) 256, 919, 940
BseYI CCCAGC 1 cut(s) 739
Bsh1236I CGCG 1 cut(s) 1515
Bsh1285I CGRYCG 1 cut(s) 2042
BshFI GGCC 7 cut(s) 390, 871, 962, 1815, 2148, 2291, 2825
BshNI GGYRCC 1 cut(s) 3046
BsiEI CGRYCG 1 cut(s) 2042
BsiHKAI GWGCWC 1 cut(s) 1479
BsiHKCI CYCGRG 1 cut(s) 93
BsiSI CCGG 8 cut(s) 94, 119, 254, 296, 728, 888, 2693, 3050
BslFI GGGAC 6 cut(s) 303, 862, 1151, 1212, 2764, 3066
BsmAI GTCTC 4 cut(s) 547, 690, 1903, 2743
BsmBI CGTCTC 1 cut(s) 547
BsmFI GGGAC 6 cut(s) 303, 862, 1151, 1212, 2764, 3066
BsmI GAATGC 1 cut(s) 1645
BsnI GGCC 7 cut(s) 390, 871, 962, 1815, 2148, 2291, 2825
Bso31I GGTCTC 2 cut(s) 1903, 2743
BsoBI CYCGRG 1 cut(s) 93
Bsp119I TTCGAA 1 cut(s) 3018
Bsp1286I GDGCHC 2 cut(s) 1419, 1479
Bsp13I TCCGGA 1 cut(s) 887
Bsp1407I TGTACA 1 cut(s) 1540
Bsp1720I GCTNAGC 1 cut(s) 1633
Bsp19I CCATGG 1 cut(s) 2892
BspACI CCGC 8 cut(s) 73, 222, 700, 1106, 1775, 2670, 2731, 2875
BspANI GGCC 7 cut(s) 390, 871, 962, 1815, 2148, 2291, 2825
BspCNI CTCAG 9 cut(s) 22, 36, 220, 265, 429, 1840, 1883, 2115, 2880
BspEI TCCGGA 1 cut(s) 887
BspFNI CGCG 1 cut(s) 1515
BspLI GGNNCC 6 cut(s) 447, 870, 2679, 2873, 3048, 3054
BspMAI CTGCAG 2 cut(s) 846, 2569
BspMI ACCTGC 1 cut(s) 1691
BspPI GGATC 6 cut(s) 1290, 1658, 1724, 2316, 2813, 2856
BspT104I TTCGAA 1 cut(s) 3018
BspT107I GGYRCC 1 cut(s) 3046
BspTNI GGTCTC 2 cut(s) 1903, 2743
BsrDI GCAATG 2 cut(s) 1693, 2923
BsrGI TGTACA 1 cut(s) 1540
BsrI ACTGG 4 cut(s) 331, 626, 1419, 1921
BssECI CCNNGG 8 cut(s) 93, 320, 872, 1134, 1428, 1429, 2784, 2892
BssNAI GTATAC 1 cut(s) 530
BssSI CACGAG 1 cut(s) 909
BssT1I CCWWGG 3 cut(s) 872, 2784, 2892
Bst1107I GTATAC 1 cut(s) 530
Bst2BI CACGAG 1 cut(s) 909
Bst2UI CCWGG 9 cut(s) 68, 191, 322, 522, 1135, 1292, 1394, 1430, 2735
Bst4CI ACNGT 7 cut(s) 464, 485, 534, 819, 1466, 1609, 2795
BstAUI TGTACA 1 cut(s) 1540
BstBAI YACGTR 1 cut(s) 58
BstBI TTCGAA 1 cut(s) 3018
BstC8I GCNNGC 7 cut(s) 1232, 1236, 1705, 1917, 2289, 2360, 2404
BstDSI CCRYGG 1 cut(s) 2892
BstFNI CGCG 1 cut(s) 1515
BstHHI GCGC 1 cut(s) 1515
BstMAI GTCTC 4 cut(s) 547, 690, 1903, 2743
BstMCI CGRYCG 1 cut(s) 2042
BstMWI GCNNNNNNNGC 4 cut(s) 25, 305, 959, 2903
BstNI CCWGG 9 cut(s) 68, 191, 322, 522, 1135, 1292, 1394, 1430, 2735
BstNSI RCATGY 5 cut(s) 1488, 1847, 2118, 2766, 2910
BstSFI CTRYAG 3 cut(s) 842, 860, 2565
BstUI CGCG 1 cut(s) 1515
BstV1I GCAGC 3 cut(s) 256, 919, 940
BstV2I GAAGAC 4 cut(s) 1917, 2496, 2517, 2926
BstX2I RGATCY 2 cut(s) 829, 856
BstXI CCANNNNNNTGG 1 cut(s) 1234
BstYI RGATCY 2 cut(s) 829, 856
BstZ17I GTATAC 1 cut(s) 530
Bsu36I CCTNAGG 1 cut(s) 2123
BsuI GTATCC 1 cut(s) 1141
BsuRI GGCC 7 cut(s) 390, 871, 962, 1815, 2148, 2291, 2825
BtgI CCRYGG 1 cut(s) 2892
BtrI CACGTC 2 cut(s) 879, 1945
BtsIMutI CAGTG 5 cut(s) 424, 490, 1024, 2110, 2514
BveI ACCTGC 1 cut(s) 1691
Cac8I GCNNGC 7 cut(s) 1232, 1236, 1705, 1917, 2289, 2360, 2404
CaiI CAGNNNCTG 1 cut(s) 632
CfoI GCGC 1 cut(s) 1515
Cfr13I GGNCC 6 cut(s) 869, 1088, 1814, 2146, 2824, 3053
Cfr9I CCCGGG 1 cut(s) 93
CseI GACGC 1 cut(s) 579
Csp6I GTAC 6 cut(s) 108, 279, 1026, 1299, 1422, 1541
CspCI CAANNNNNGTGG 2 cut(s) 331, 366
CviQI GTAC 6 cut(s) 108, 279, 1026, 1299, 1422, 1541
DraI TTTAAA 1 cut(s) 679
DraIII CACNNNGTG 2 cut(s) 1589, 2444
EaeI YGGCCR 1 cut(s) 2289
Ecl136II GAGCTC 1 cut(s) 1477
Eco130I CCWWGG 3 cut(s) 872, 2784, 2892
Eco24I GRGCYC 2 cut(s) 1419, 1479
Eco31I GGTCTC 2 cut(s) 1903, 2743
Eco32I GATATC 1 cut(s) 2242
Eco47I GGWCC 2 cut(s) 1088, 3053
Eco53kI GAGCTC 1 cut(s) 1477
Eco57I CTGAAG 3 cut(s) 1086, 1487, 2490
Eco81I CCTNAGG 1 cut(s) 2123
Eco88I CYCGRG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 1477
EcoO109I RGGNCCY 1 cut(s) 869
EcoRI GAATTC 3 cut(s) 1491, 2053, 3020
EcoRII CCWGG 9 cut(s) 66, 189, 320, 520, 1133, 1290, 1392, 1428, 2733
EcoRV GATATC 1 cut(s) 2242
EcoT14I CCWWGG 3 cut(s) 872, 2784, 2892
EcoT22I ATGCAT 3 cut(s) 576, 796, 2908
EcoT38I GRGCYC 2 cut(s) 1419, 1479
ErhI CCWWGG 3 cut(s) 872, 2784, 2892
Esp3I CGTCTC 1 cut(s) 547
FalI AAGNNNNNCTT 6 cut(s) 672, 704, 2082, 2114, 2768, 2800
FaqI GGGAC 6 cut(s) 303, 862, 1151, 1212, 2764, 3066
FauI CCCGC 1 cut(s) 215
FauNDI CATATG 2 cut(s) 766, 1042
FbaI TGATCA 3 cut(s) 421, 757, 2482
FblI GTMKAC 2 cut(s) 529, 620
Fnu4HI GCNGC 3 cut(s) 270, 933, 954
FriOI GRGCYC 2 cut(s) 1419, 1479
Fsp4HI GCNGC 3 cut(s) 270, 933, 954
FspBI CTAG 2 cut(s) 1361, 1548
GlaI GCGC 1 cut(s) 1514
GluI GCNGC 3 cut(s) 270, 933, 954
GsaI CCCAGC 1 cut(s) 743
GsuI CTGGAG 3 cut(s) 50, 609, 1136
HaeIII GGCC 7 cut(s) 390, 871, 962, 1815, 2148, 2291, 2825
HapII CCGG 8 cut(s) 94, 119, 254, 296, 728, 888, 2693, 3050
HgaI GACGC 1 cut(s) 579
HhaI GCGC 1 cut(s) 1515
Hin6I GCGC 1 cut(s) 1513
HinP1I GCGC 1 cut(s) 1513
HincII GTYRAC 1 cut(s) 477
HindII GTYRAC 1 cut(s) 477
HindIII AAGCTT 4 cut(s) 566, 1715, 1913, 2188
HpaII CCGG 8 cut(s) 94, 119, 254, 296, 728, 888, 2693, 3050
HphI GGTGA 6 cut(s) 179, 358, 614, 1601, 1672, 2807
Hpy166II GTNNAC 6 cut(s) 460, 477, 530, 621, 1028, 1424
Hpy8I GTNNAC 6 cut(s) 460, 477, 530, 621, 1028, 1424
HpyAV CCTTC 8 cut(s) 422, 535, 1110, 1297, 1511, 1616, 2348, 2792
HpyCH4III ACNGT 7 cut(s) 464, 485, 534, 819, 1466, 1609, 2795
HpyCH4IV ACGT 6 cut(s) 57, 556, 878, 977, 1944, 3060
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 305, 959, 2903
HpySE526I ACGT 6 cut(s) 57, 556, 878, 977, 1944, 3060
HspAI GCGC 1 cut(s) 1513
Kpn2I TCCGGA 1 cut(s) 887
Ksp22I TGATCA 3 cut(s) 421, 757, 2482
LmnI GCTCC 8 cut(s) 166, 199, 655, 970, 1510, 1973, 2123, 2623
Lsp1109I GCAGC 3 cut(s) 256, 919, 940
MaeI CTAG 2 cut(s) 1361, 1548
MaeII ACGT 6 cut(s) 57, 556, 878, 977, 1944, 3060
MaeIII GTNAC 5 cut(s) 185, 1363, 2662, 2758, 2968
MflI RGATCY 2 cut(s) 829, 856
MhlI GDGCHC 2 cut(s) 1419, 1479
MlsI TGGCCA 1 cut(s) 2291
MluNI TGGCCA 1 cut(s) 2291
MlyI GAGTC 5 cut(s) 55, 692, 1584, 2145, 2997
MmeI TCCRAC 3 cut(s) 1488, 1999, 2601
Mox20I TGGCCA 1 cut(s) 2291
Mph1103I ATGCAT 3 cut(s) 576, 796, 2908
MroI TCCGGA 1 cut(s) 887
MroXI GAANNNNTTC 1 cut(s) 2094
MscI TGGCCA 1 cut(s) 2291
MseI TTAA 9 cut(s) 678, 789, 948, 1013, 1143, 1863, 2580, 2655, 2943
MslI CAYNNNNRTG 4 cut(s) 192, 1163, 2856, 2911
Msp20I TGGCCA 1 cut(s) 2291
MspA1I CMGCKG 2 cut(s) 28, 1106
MspI CCGG 8 cut(s) 94, 119, 254, 296, 728, 888, 2693, 3050
Mva1269I GAATGC 1 cut(s) 1645
MvaI CCWGG 9 cut(s) 68, 191, 322, 522, 1135, 1292, 1394, 1430, 2735
MvnI CGCG 1 cut(s) 1515
MwoI GCNNNNNNNGC 4 cut(s) 25, 305, 959, 2903
NciI CCSGG 4 cut(s) 94, 95, 119, 3051
NcoI CCATGG 1 cut(s) 2892
NdeI CATATG 2 cut(s) 766, 1042
NlaIV GGNNCC 6 cut(s) 447, 870, 2679, 2873, 3048, 3054
NmuCI GTSAC 2 cut(s) 185, 2968
NsiI ATGCAT 3 cut(s) 576, 796, 2908
NspI RCATGY 5 cut(s) 1488, 1847, 2118, 2766, 2910
NspV TTCGAA 1 cut(s) 3018
OliI CACNNNNGTG 1 cut(s) 2856
PaqCI CACCTGC 1 cut(s) 1691
PasI CCCWGGG 1 cut(s) 1429
PciI ACATGT 1 cut(s) 1484
PcsI WCGNNNNNNNCGW 1 cut(s) 1189
PctI GAATGC 1 cut(s) 1645
PdmI GAANNNNTTC 1 cut(s) 2094
PfeI GAWTC 9 cut(s) 250, 605, 733, 748, 1259, 1950, 2032, 2519, 3037
PflFI GACNNNGTC 1 cut(s) 2966
PflMI CCANNNNNTGG 1 cut(s) 1435
PkrI GCNGC 3 cut(s) 271, 934, 955
Ple19I CGATCG 1 cut(s) 2042
PleI GAGTC 5 cut(s) 54, 691, 1583, 2145, 2997
PpsI GAGTC 5 cut(s) 54, 691, 1583, 2145, 2997
Ppu21I YACGTR 1 cut(s) 58
PscI ACATGT 1 cut(s) 1484
PshBI ATTAAT 1 cut(s) 1013
PsiI TTATAA 1 cut(s) 1521
Psp124BI GAGCTC 1 cut(s) 1479
Psp6I CCWGG 9 cut(s) 66, 189, 320, 520, 1133, 1290, 1392, 1428, 2733
PspFI CCCAGC 1 cut(s) 739
PspGI CCWGG 9 cut(s) 66, 189, 320, 520, 1133, 1290, 1392, 1428, 2733
PspN4I GGNNCC 6 cut(s) 447, 870, 2679, 2873, 3048, 3054
PspPI GGNCC 6 cut(s) 869, 1088, 1814, 2146, 2824, 3053
PstI CTGCAG 2 cut(s) 846, 2569
PstNI CAGNNNCTG 1 cut(s) 632
PsuI RGATCY 2 cut(s) 829, 856
PsyI GACNNNGTC 1 cut(s) 2966
PvuI CGATCG 1 cut(s) 2042
PvuII CAGCTG 1 cut(s) 28
RgaI GCGATCGC 1 cut(s) 2042
RsaI GTAC 6 cut(s) 109, 280, 1027, 1300, 1423, 1542
RsaNI GTAC 6 cut(s) 108, 279, 1026, 1299, 1422, 1541
RseI CAYNNNNRTG 4 cut(s) 192, 1163, 2856, 2911
SacI GAGCTC 1 cut(s) 1479
SaqAI TTAA 9 cut(s) 678, 789, 948, 1013, 1143, 1863, 2580, 2655, 2943
SatI GCNGC 3 cut(s) 270, 933, 954
Sau96I GGNCC 6 cut(s) 869, 1088, 1814, 2146, 2824, 3053
ScaI AGTACT 1 cut(s) 280
SchI GAGTC 5 cut(s) 55, 692, 1584, 2145, 2997
SduI GDGCHC 2 cut(s) 1419, 1479
SfaAI GCGATCGC 1 cut(s) 2042
SfcI CTRYAG 3 cut(s) 842, 860, 2565
SfuI TTCGAA 1 cut(s) 3018
SgfI GCGATCGC 1 cut(s) 2042
SinI GGWCC 2 cut(s) 1088, 3053
SmaI CCCGGG 1 cut(s) 95
SmiMI CAYNNNNRTG 4 cut(s) 192, 1163, 2856, 2911
SmlI CTYRAG 7 cut(s) 440, 689, 1202, 1751, 2185, 2534, 3005
SmoI CTYRAG 7 cut(s) 440, 689, 1202, 1751, 2185, 2534, 3005
SsiI CCGC 8 cut(s) 73, 222, 700, 1106, 1775, 2670, 2731, 2875
SspI AATATT 1 cut(s) 3015
SspMI CTAG 2 cut(s) 1361, 1548
SstI GAGCTC 1 cut(s) 1479
StyI CCWWGG 3 cut(s) 872, 2784, 2892
TaaI ACNGT 7 cut(s) 464, 485, 534, 819, 1466, 1609, 2795
TaiI ACGT 6 cut(s) 60, 559, 881, 980, 1947, 3063
TaqI TCGA 7 cut(s) 248, 1192, 2311, 2607, 2880, 3001, 3018
TaqII GACCGA 1 cut(s) 1839
TatI WGTACW 4 cut(s) 278, 1025, 1421, 1540
TfiI GAWTC 9 cut(s) 250, 605, 733, 748, 1259, 1950, 2032, 2519, 3037
Tru1I TTAA 9 cut(s) 678, 789, 948, 1013, 1143, 1863, 2580, 2655, 2943
Tru9I TTAA 9 cut(s) 678, 789, 948, 1013, 1143, 1863, 2580, 2655, 2943
TscAI CASTG 5 cut(s) 424, 490, 1024, 2110, 2521
TseFI GTSAC 2 cut(s) 185, 2968
TseI GCWGC 3 cut(s) 269, 932, 953
Tsp45I GTSAC 2 cut(s) 185, 2968
TspGWI ACGGA 1 cut(s) 1923
TspMI CCCGGG 1 cut(s) 93
TspRI CASTG 5 cut(s) 424, 490, 1024, 2110, 2521
Tth111I GACNNNGTC 1 cut(s) 2966
Van91I CCANNNNNTGG 1 cut(s) 1435
VpaK11BI GGWCC 2 cut(s) 1088, 3053
VspI ATTAAT 1 cut(s) 1013
XceI RCATGY 5 cut(s) 1488, 1847, 2118, 2766, 2910
XcmI CCANNNNNNNNNTGG 1 cut(s) 1375
XmaI CCCGGG 1 cut(s) 93
XmiI GTMKAC 2 cut(s) 529, 620
XmnI GAANNNNTTC 1 cut(s) 2094
XspI CTAG 2 cut(s) 1361, 1548
ZrmI AGTACT 1 cut(s) 280
Zsp2I ATGCAT 3 cut(s) 576, 796, 2908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.