Rh7CG131100

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0011354
Physical Location & Seq
Reverse (-)
10350912 .. 10356162
5251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG131100.1

Sequence Viewer

Length: 3069 bp
ATGAGGCTTTACGTGTATGTGATGGAAGCTCATGACTTGGTTGTGAAGGACTCTTGCTTTGTGGAGCTTCAGGTTGGGAAGCACAAGTCCAAGACTAGGATTTTGAGGAACACCAACAACCCTGCTTGGAATGAAGAGTTTGTGTTCAGAGTGTATGATATGAATGAGGAGCTACTTGTGTCTGTTTTTCACCAGGATGATGACTCTAATGGTCTGTTTAATGGGGCGGAGTTAGTCGGCCGGGTTCGGATTCCGGTGGGGTCTGTTGCTGCTGAGAATAATAGTACCTTGCCGCCCGCATGGTATTCTTTTGAAAGGCCCAAGTCCGGGAAGTTCATCAATACAGATTGTGGAAAAATTCTTCTCACTCTATCGTTGCATGAAAAAGGCCATGATGCCGCTGCTAATGACCTCCCTTGTTTGCACTCAAATATAACTGAGGAGCCTAAAGAGATTGACAGTCCATTTGTATCATATAATGGTGTCTTATGTTCCAAATCTCCATGTGGAAAGACACCGGGAGGAAAACAATGGATGAAGGCTCTTAGGAGACGTATGGACAGGCTTCTTCATAAGAATGATGAAGCTTCAAGAACTGATGATTCCTCAGAGCTGTCTAGTTCAGTATCTGACTATGAAGATTGCATGGAGGAACAGCATTCTAATTGTAGCTTTGAAAAGTCTCTTGAAATGATGCAGTCAAGTGAGAGCGAACAAAAAATGCCAGAGAATCTTCTGGGGGGATTTCTTATTGATCAGGCTTATGTTGTTTCACCAAGTGATCTTAATGCATCTCTTTTTACCCCAAATTCACAGTTTAGGAGAGATTTAGCAGAACTGCAGCGGACAACAGATGTACATGAGGGGCCTTGGACATGGAAATCAGGAGAGATGTCATGTTTAACACGGGTAGTTACCTACATAAATGCTGCAACAAAGTTGGTTAAAGCTGTTAAAGCCACAGAGGAGCAAACATATCTCACAGCAAACGGAAGGGAATTTGCTGTATTGGCAAGTGTTTGTACACCCGAAGTTCCATATGGAAGTTCATTCAAGGTTGAATTGCTTTACAAGATATTGCCTGGACCAGAGCTACCTTCAGGGGAAGAATCTTCTCACCTTGTGGTATCCTGGGAAGTTAACTTTCTCCATAGCACATTGATGAAAGGCATGATTGAAGGAGGAGTTCGACAGGGACTTAAGGACAGTTTCGAGCAGTTCTCCATCTTGATGGCTCAAGAGTTTAAAGTGCTGGATTCTACAGATTTGTCTGACAAGGATCATATCCTGGCCAATATGCAGGCAGGACACCAATCAGATTGGGAATTGGCAAAGGAATACTTCTGGAATATGACTGTTGTTTCCACTATTTTTATGGTTTCATATATCATGGTGCACATTTTACTTTGTGAACCAAGTAAACTCCAAGGCCTAGAATTTCATGGACTTGATTTACCAGATAGTATCGGAGAGTTTATCACATGTGGAATTCTAGTCCTTCAATTGGAGCGCATTTATAATATGGTTCAACACTTTGTGCAAGCTAGGTTGCAACAAGGAAGTGATCATGGAGTCAAATCCCAAGGTGATGGATGGGTTCTCACTGTTGCCTTGATTGAAGGGACAAACCTAGCCTCCTTGGATTCATCATCAGGGTTCTCTGATCCCTATGTGGTTTTCACCAGCAATGGTAAAACAAGAACAAGCTCTGTCAGGCTTCAAACTACTGATCCTCAATGGAATGACATACTTGAGTTCGATGCTATGGAAGAACCCCCATCAGTTCTAGACGTGGAAGTTTTTGATTTTGATGGCCCTTTTGACCAAGCCATGTCTCTAGGGCATGCTGAGATCAATTTTCTGAAACACTCAGCTACTGAACTCGCAGACATGTGGGTCTCCCTTGAGGGAAAGCTTGCTCAGTCTTCTCAATCAAAGCTGCACTTGAGAATCTTTCTGGACAATAACAAAGGACTTGATACAATTAAGGAGTATATGACCAAAATGGAAAAGGAGGTTGGAAAGAAGTTGGATCTCCGATCGCCTCATAGGAATTCTACATTTCAGAAATTGTTTGGGTTGCCACCAGAAGAATTTCTCGTCAGTGATTTTACATGTGCACTGAAAAGGAAAATGCCAGTGCAGGGCCGACTTTTTCTATCTGCAAGAATAGTCGGGTTTTATGCAAACTTGTTTGGACACAAAACAAAATTTTTCTTTCTTTGGGAGGATATTGAAGATATCCAAGAGCATCCACCCTCCCTATCATCTGTGGGAAGCCCTTTGCTGGTCATTGTTTTGAAAAAGGATCGAGGTATTGATGCAAGGCATGGGGCGAAGTGCCAAGATGAAGAAGGCCGGCTTAGATTTTATTTCCAATCATTCTTGTCGTTCAGTTCAGCAAGCAGGATGATAGTGGGCTTGTGGAGAACAAGAGCACTAAGTCCAGATCAGAAAGCACAAATTGCTGAAGATCTGGATGATCATGAAGAAAGGTCCAAGATGCTTGAAGATACTGAATCCATACTCAATCTTGAAGATACAAAGATGTCTAAAGTTTATACTGCAGAAATACCTGTGAATACAAAATCTTTGATGGAAATGTTCAATGGAGGAAAGCTGGAGCACAAAATTATGGAGAAATCAGGTTGTCTTCACTATGCAACTACTGCATGGGAACCTATAAAGCCAAATATCTTTGAGCGACGTTTGTCTTATAGATTCAACCGCAATGTTTCCATCTTTGGGGGAGAGGTGGCATGCAGACAACGAAAGTCTCCAAATGCAAATGGTGAGGGATGGATCATCGATGAAGTTATGGCCCTGTACGGTGTTCCATTTGGCGATCATTTTCGGGTTCAGTTAAGGTACCGGATTGAGAAATCTGCCCTTGCTCATAATGCATGTAAATGCAATGTCTATGTCAGGGTCACCTGGCTCAAGAGCACGATGTTTCTGGAAAGGATCAAACACAACATAACTGGGAAGTTTGCTCATAGACTGAAGGATATATTTGAAATGGTAGAGAGAGAGATTCTCTTGACACCTCAACAGGACATTGGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1022

Amino Acids

115.91

Weight (kDa)

5.6

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 102 2e-17 C2 domain
VASt PF16016 250 - 399 1.2e-28 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 533 - 635 4.7e-14 C2 domain
GRAM PF02893 687 - 777 1.1e-16 GRAM domain
VASt PF16016 853 - 992 1e-23 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011354)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50170
fragaria_vesca FvH4_5g06260 FvH4_5g06260 FvH4_5g06260
malus_domestica MD06G1166100.v1.1
prunus_persica Prupe.5G166000_v2.0.a1
pyrus_communis pycom06g14860
rosa_chinensis RchiOBHm_Chr7g0192601
rosa_laevigata RLG00000004348
rosa_multiflora Rmu_sc0004161.1_g000002
rosa_roxburghii Rroxscaffold_3G00262850
rosa_rugosa Rorug07G0002600 Rorug07G0002700 Rorug07G0002800
rosa_samantha Rh7AG127000 Rh7BG129600 Rh7CG131100 Rh7CG132000 Rh7DG130600
rosa_wichuraiana Rw7G010940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1518
AasI GACNNNNNNGTC 1 cut(s) 1895
Acc65I GGTACC 1 cut(s) 2868
AccB1I GGYRCC 1 cut(s) 2868
AciI CCGC 6 cut(s) 227, 293, 297, 399, 844, 2728
AclWI GGATC 7 cut(s) 1287, 1658, 1724, 2040, 2316, 2810, 2972
AcoI YGGCCR 2 cut(s) 238, 1290
AcsI RAATTY 8 cut(s) 357, 808, 998, 1436, 1488, 2053, 2093, 2210
AcuI CTGAAG 4 cut(s) 53, 1083, 2490, 3023
AdeI CACNNNGTG 3 cut(s) 779, 1123, 1537
AfaI GTAC 5 cut(s) 286, 858, 1024, 2828, 2870
AfiI CCNNNNNNNGG 7 cut(s) 96, 326, 327, 1504, 2144, 2287, 2745
AflII CTTAAG 1 cut(s) 1199
AflIII ACRYGT 4 cut(s) 12, 1481, 1890, 2114
AjiI CACGTC 1 cut(s) 1792
AjnI CCWGG 5 cut(s) 192, 1081, 1130, 1287, 2933
AjuI GAANNNNNNNTTGG 2 cut(s) 2001, 2033
Alw21I GWGCWC 5 cut(s) 1398, 2122, 2440, 2628, 2948
Alw26I GTCTC 5 cut(s) 544, 687, 1839, 1903, 2781
Alw44I GTGCAC 2 cut(s) 1394, 2118
AlwI GGATC 7 cut(s) 1287, 1658, 1724, 2040, 2316, 2810, 2972
AlwNI CAGNNNCTG 2 cut(s) 629, 1877
ApaLI GTGCAC 2 cut(s) 1394, 2118
ApeKI GCWGC 5 cut(s) 269, 401, 841, 929, 1939
ApoI RAATTY 8 cut(s) 357, 808, 998, 1436, 1488, 2053, 2093, 2210
ArsI GACNNNNNNTTYG 6 cut(s) 41, 73, 85, 117, 449, 481
Asp700I GAANNNNTTC 1 cut(s) 2094
Asp718I GGTACC 1 cut(s) 2868
AspLEI GCGC 1 cut(s) 1512
AspS9I GGNCC 7 cut(s) 318, 866, 1085, 1814, 2146, 2496, 2821
AsuC2I CCSGG 3 cut(s) 242, 328, 519
AsuHPI GGTGA 7 cut(s) 182, 765, 1109, 1598, 1672, 2804, 2923
AvaII GGWCC 2 cut(s) 1085, 2496
BaeGI GKGCMC 2 cut(s) 1398, 2122
BalI TGGCCA 1 cut(s) 1292
BanI GGYRCC 1 cut(s) 2868
BbsI GAAGAC 2 cut(s) 1917, 2645
Bbv12I GWGCWC 5 cut(s) 1398, 2122, 2440, 2628, 2948
BbvI GCAGC 5 cut(s) 256, 388, 853, 916, 1926
BciT130I CCWGG 5 cut(s) 194, 1083, 1132, 1289, 2935
BciVI GTATCC 1 cut(s) 1138
BclI TGATCA 3 cut(s) 754, 1564, 2482
BcnI CCSGG 3 cut(s) 242, 328, 519
BcoDI GTCTC 5 cut(s) 544, 687, 1839, 1903, 2781
BfaI CTAG 8 cut(s) 96, 618, 1433, 1493, 1545, 1631, 1787, 1838
BfmI CTRYAG 3 cut(s) 839, 1260, 2565
BfrI CTTAAG 1 cut(s) 1199
BfuI GTATCC 1 cut(s) 1138
BglII AGATCT 1 cut(s) 2473
BisI GCNGC 7 cut(s) 270, 293, 399, 402, 842, 930, 1940
BlsI GCNGC 7 cut(s) 271, 294, 400, 403, 843, 931, 1941
Bme1390I CCNGG 8 cut(s) 194, 242, 328, 519, 1083, 1132, 1289, 2935
Bme18I GGWCC 2 cut(s) 1085, 2496
BmgBI CACGTC 1 cut(s) 1792
BmgT120I GGNCC 7 cut(s) 318, 866, 1085, 1814, 2146, 2496, 2821
BmiI GGNNCC 4 cut(s) 444, 867, 2679, 2870
BmrFI CCNGG 8 cut(s) 194, 242, 328, 519, 1083, 1132, 1289, 2935
BmrI ACTGGG 1 cut(s) 2991
BmsI GCATC 7 cut(s) 385, 684, 800, 1750, 2260, 2311, 2493
BmuI ACTGGG 1 cut(s) 2991
BoxI GACNNNNGTC 1 cut(s) 2710
BpiI GAAGAC 2 cut(s) 1917, 2645
BplI GAGNNNNNCTC 4 cut(s) 1205, 1237, 3021, 3053
BpmI CTGGAG 1 cut(s) 2642
BpuEI CTTGAG 5 cut(s) 1221, 1772, 1925, 1966, 2924
BpuMI CCSGG 3 cut(s) 242, 328, 519
Bsa29I ATCGAT 1 cut(s) 2808
BsaAI YACGTR 1 cut(s) 13
BsaI GGTCTC 1 cut(s) 1903
BsaJI CCNNGG 5 cut(s) 869, 1131, 1426, 1582, 1638
BsaWI WCCGGW 2 cut(s) 253, 2871
BsaXI ACNNNNNCTCC 2 cut(s) 1619, 1649
Bsc4I CCNNNNNNNGG 7 cut(s) 96, 326, 327, 1504, 2144, 2287, 2745
Bse118I RCCGGY 1 cut(s) 2358
Bse1I ACTGG 2 cut(s) 2138, 2986
Bse3DI GCAATG 3 cut(s) 1693, 2737, 2920
BseBI CCWGG 5 cut(s) 194, 1083, 1132, 1289, 2935
BseCI ATCGAT 1 cut(s) 2808
BseDI CCNNGG 5 cut(s) 869, 1131, 1426, 1582, 1638
BseGI GGATG 7 cut(s) 202, 540, 1598, 2251, 2415, 2485, 2804
BseLI CCNNNNNNNGG 7 cut(s) 96, 326, 327, 1504, 2144, 2287, 2745
BseMI GCAATG 3 cut(s) 1693, 2737, 2920
BseMII CTCAG 6 cut(s) 264, 429, 621, 1839, 1884, 1934
BseNI ACTGG 2 cut(s) 2138, 2986
BseRI GAGGAG 4 cut(s) 182, 455, 980, 1197
BseSI GKGCMC 2 cut(s) 1398, 2122
BseX3I CGGCCG 1 cut(s) 238
BseXI GCAGC 5 cut(s) 256, 388, 853, 916, 1926
BsgI GTGCAG 2 cut(s) 1925, 2162
Bsh1285I CGRYCG 2 cut(s) 241, 2042
BshNI GGYRCC 1 cut(s) 2868
BshVI ATCGAT 1 cut(s) 2808
BsiEI CGRYCG 2 cut(s) 241, 2042
BsiHKAI GWGCWC 5 cut(s) 1398, 2122, 2440, 2628, 2948
BsiSI CCGG 6 cut(s) 241, 254, 327, 518, 2359, 2872
BslFI GGGAC 2 cut(s) 1209, 1636
BslI CCNNNNNNNGG 7 cut(s) 96, 326, 327, 1504, 2144, 2287, 2745
BsmAI GTCTC 5 cut(s) 544, 687, 1839, 1903, 2781
BsmBI CGTCTC 1 cut(s) 544
BsmFI GGGAC 2 cut(s) 1209, 1636
BsmI GAATGC 1 cut(s) 658
Bso31I GGTCTC 1 cut(s) 1903
Bsp1286I GDGCHC 5 cut(s) 1398, 2122, 2440, 2628, 2948
Bsp1407I TGTACA 2 cut(s) 856, 1022
BspACI CCGC 6 cut(s) 227, 293, 297, 399, 844, 2728
BspCNI CTCAG 6 cut(s) 265, 430, 620, 1840, 1883, 1933
BspDI ATCGAT 1 cut(s) 2808
BspHI TCATGA 2 cut(s) 31, 2485
BspLI GGNNCC 4 cut(s) 444, 867, 2679, 2870
BspMAI CTGCAG 2 cut(s) 843, 2569
BspPI GGATC 7 cut(s) 1287, 1658, 1724, 2040, 2316, 2810, 2972
BspT107I GGYRCC 1 cut(s) 2868
BspTI CTTAAG 1 cut(s) 1199
BspTNI GGTCTC 1 cut(s) 1903
BsrDI GCAATG 3 cut(s) 1693, 2737, 2920
BsrFI RCCGGY 1 cut(s) 2358
BsrGI TGTACA 2 cut(s) 856, 1022
BsrI ACTGG 2 cut(s) 2138, 2986
BssAI RCCGGY 1 cut(s) 2358
BssECI CCNNGG 5 cut(s) 869, 1131, 1426, 1582, 1638
BssT1I CCWWGG 4 cut(s) 869, 1426, 1582, 1638
Bst2UI CCWGG 5 cut(s) 194, 1083, 1132, 1289, 2935
Bst4CI ACNGT 6 cut(s) 461, 816, 1208, 1357, 1606, 2831
Bst6I CTCTTC 1 cut(s) 129
BstAFI CTTAAG 1 cut(s) 1199
BstAPI GCANNNNNTGC 2 cut(s) 2465, 2669
BstAUI TGTACA 2 cut(s) 856, 1022
BstBAI YACGTR 1 cut(s) 13
BstC8I GCNNGC 8 cut(s) 297, 1302, 1542, 1845, 1917, 2360, 2404, 2761
BstDEI CTNAG 9 cut(s) 273, 438, 545, 607, 1848, 1870, 1920, 2363, 2441
BstEII GGTNACC 1 cut(s) 2929
BstF5I GGATG 7 cut(s) 202, 540, 1598, 2251, 2415, 2485, 2804
BstHHI GCGC 1 cut(s) 1512
BstMAI GTCTC 5 cut(s) 544, 687, 1839, 1903, 2781
BstMCI CGRYCG 2 cut(s) 241, 2042
BstMWI GCNNNNNNNGC 5 cut(s) 956, 1010, 2465, 2669, 2900
BstNI CCWGG 5 cut(s) 194, 1083, 1132, 1289, 2935
BstNSI RCATGY 6 cut(s) 1485, 1847, 1894, 2118, 2763, 2907
BstPAI GACNNNNGTC 1 cut(s) 2710
BstPI GGTNACC 1 cut(s) 2929
BstSCI CCNGG 8 cut(s) 192, 240, 326, 517, 1081, 1130, 1287, 2933
BstSFI CTRYAG 3 cut(s) 839, 1260, 2565
BstSLI GKGCMC 2 cut(s) 1398, 2122
BstV1I GCAGC 5 cut(s) 256, 388, 853, 916, 1926
BstV2I GAAGAC 2 cut(s) 1917, 2645
BstX2I RGATCY 2 cut(s) 2032, 2473
BstXI CCANNNNNNTGG 2 cut(s) 1231, 1589
BstYI RGATCY 2 cut(s) 2032, 2473
BstZI CGGCCG 1 cut(s) 238
Bsu15I ATCGAT 1 cut(s) 2808
BsuI GTATCC 1 cut(s) 1138
BsuTUI ATCGAT 1 cut(s) 2808
BtrI CACGTC 1 cut(s) 1792
BtsCI GGATG 7 cut(s) 202, 540, 1598, 2251, 2415, 2485, 2804
BtsIMutI CAGTG 4 cut(s) 1602, 2110, 2120, 2145
Cac8I GCNNGC 8 cut(s) 297, 1302, 1542, 1845, 1917, 2360, 2404, 2761
CaiI CAGNNNCTG 2 cut(s) 629, 1877
CciI TCATGA 2 cut(s) 31, 2485
CfoI GCGC 1 cut(s) 1512
Cfr10I RCCGGY 1 cut(s) 2358
Cfr13I GGNCC 7 cut(s) 318, 866, 1085, 1814, 2146, 2496, 2821
ClaI ATCGAT 1 cut(s) 2808
Csp6I GTAC 5 cut(s) 285, 857, 1023, 2827, 2869
CviQI GTAC 5 cut(s) 285, 857, 1023, 2827, 2869
DdeI CTNAG 9 cut(s) 273, 438, 545, 607, 1848, 1870, 1920, 2363, 2441
DraI TTTAAA 1 cut(s) 1246
DraIII CACNNNGTG 3 cut(s) 779, 1123, 1537
DrdI GACNNNNNNGTC 1 cut(s) 1895
DseDI GACNNNNNNGTC 1 cut(s) 1895
EaeI YGGCCR 2 cut(s) 238, 1290
EagI CGGCCG 1 cut(s) 238
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
EciI GGCGGA 1 cut(s) 242
EclXI CGGCCG 1 cut(s) 238
Eco130I CCWWGG 4 cut(s) 869, 1426, 1582, 1638
Eco147I AGGCCT 1 cut(s) 1431
Eco31I GGTCTC 1 cut(s) 1903
Eco32I GATATC 1 cut(s) 2242
Eco47I GGWCC 2 cut(s) 1085, 2496
Eco52I CGGCCG 1 cut(s) 238
Eco57I CTGAAG 4 cut(s) 53, 1083, 2490, 3023
Eco91I GGTNACC 1 cut(s) 2929
EcoO109I RGGNCCY 1 cut(s) 866
EcoO65I GGTNACC 1 cut(s) 2929
EcoRI GAATTC 2 cut(s) 1488, 2053
EcoRII CCWGG 5 cut(s) 192, 1081, 1130, 1287, 2933
EcoRV GATATC 1 cut(s) 2242
EcoT14I CCWWGG 4 cut(s) 869, 1426, 1582, 1638
EcoT22I ATGCAT 2 cut(s) 793, 2905
ErhI CCWWGG 4 cut(s) 869, 1426, 1582, 1638
Esp3I CGTCTC 1 cut(s) 544
FalI AAGNNNNNCTT 6 cut(s) 1325, 1357, 1928, 1960, 2346, 2378
FaqI GGGAC 2 cut(s) 1209, 1636
FauI CCCGC 1 cut(s) 304
FauNDI CATATG 1 cut(s) 1039
FbaI TGATCA 3 cut(s) 754, 1564, 2482
Fnu4HI GCNGC 7 cut(s) 270, 293, 399, 402, 842, 930, 1940
FokI GGATG 7 cut(s) 209, 547, 1605, 2238, 2422, 2492, 2811
Fsp4HI GCNGC 7 cut(s) 270, 293, 399, 402, 842, 930, 1940
FspBI CTAG 8 cut(s) 96, 618, 1433, 1493, 1545, 1631, 1787, 1838
GlaI GCGC 1 cut(s) 1511
GluI GCNGC 7 cut(s) 270, 293, 399, 402, 842, 930, 1940
GsuI CTGGAG 1 cut(s) 2642
HapII CCGG 6 cut(s) 241, 254, 327, 518, 2359, 2872
HhaI GCGC 1 cut(s) 1512
Hin6I GCGC 1 cut(s) 1510
HinP1I GCGC 1 cut(s) 1510
HincII GTYRAC 1 cut(s) 1141
HindII GTYRAC 1 cut(s) 1141
HindIII AAGCTT 2 cut(s) 585, 1913
HpaI GTTAAC 1 cut(s) 1141
HpaII CCGG 6 cut(s) 241, 254, 327, 518, 2359, 2872
HphI GGTGA 7 cut(s) 182, 765, 1109, 1598, 1672, 2804, 2923
Hpy166II GTNNAC 6 cut(s) 1025, 1141, 1396, 1412, 1421, 2120
Hpy8I GTNNAC 6 cut(s) 1025, 1141, 1396, 1412, 1421, 2120
Hpy99I CGWCG 1 cut(s) 2709
HpyAV CCTTC 9 cut(s) 40, 532, 987, 1107, 1172, 1508, 1613, 2348, 2998
HpyCH4III ACNGT 6 cut(s) 461, 816, 1208, 1357, 1606, 2831
HpyCH4IV ACGT 4 cut(s) 12, 553, 1791, 2707
HpyF10VI GCNNNNNNNGC 5 cut(s) 956, 1010, 2465, 2669, 2900
HpyF3I CTNAG 9 cut(s) 273, 438, 545, 607, 1848, 1870, 1920, 2363, 2441
HpySE526I ACGT 4 cut(s) 12, 553, 1791, 2707
HspAI GCGC 1 cut(s) 1510
KpnI GGTACC 1 cut(s) 2872
KroI GCCGGC 1 cut(s) 2358
KroNI GCCGGC 1 cut(s) 2360
Ksp22I TGATCA 3 cut(s) 754, 1564, 2482
KspAI GTTAAC 1 cut(s) 1141
LmnI GCTCC 6 cut(s) 64, 169, 442, 967, 1507, 2623
Lsp1109I GCAGC 5 cut(s) 256, 388, 853, 916, 1926
LweI GCATC 7 cut(s) 385, 684, 800, 1750, 2260, 2311, 2493
MaeI CTAG 8 cut(s) 96, 618, 1433, 1493, 1545, 1631, 1787, 1838
MaeII ACGT 4 cut(s) 12, 553, 1791, 2707
MaeIII GTNAC 2 cut(s) 913, 2929
MfeI CAATTG 1 cut(s) 1502
MflI RGATCY 2 cut(s) 2032, 2473
MhlI GDGCHC 5 cut(s) 1398, 2122, 2440, 2628, 2948
MlsI TGGCCA 1 cut(s) 1292
MluNI TGGCCA 1 cut(s) 1292
MlyI GAGTC 3 cut(s) 44, 197, 1581
MmeI TCCRAC 2 cut(s) 1999, 2010
Mox20I TGGCCA 1 cut(s) 1292
Mph1103I ATGCAT 2 cut(s) 793, 2905
MroNI GCCGGC 1 cut(s) 2358
MroXI GAANNNNTTC 1 cut(s) 2094
MscI TGGCCA 1 cut(s) 1292
MslI CAYNNNNRTG 5 cut(s) 195, 576, 1160, 1229, 2908
Msp20I TGGCCA 1 cut(s) 1292
MspA1I CMGCKG 2 cut(s) 401, 844
MspCI CTTAAG 1 cut(s) 1199
MspI CCGG 6 cut(s) 241, 254, 327, 518, 2359, 2872
MspR9I CCNGG 8 cut(s) 194, 242, 328, 519, 1083, 1132, 1289, 2935
MunI CAATTG 1 cut(s) 1502
Mva1269I GAATGC 1 cut(s) 658
MvaI CCWGG 5 cut(s) 194, 1083, 1132, 1289, 2935
MwoI GCNNNNNNNGC 5 cut(s) 956, 1010, 2465, 2669, 2900
NaeI GCCGGC 1 cut(s) 2360
NciI CCSGG 3 cut(s) 242, 328, 519
NdeI CATATG 1 cut(s) 1039
NgoMIV GCCGGC 1 cut(s) 2358
NlaIV GGNNCC 4 cut(s) 444, 867, 2679, 2870
NmuCI GTSAC 1 cut(s) 2929
NsiI ATGCAT 2 cut(s) 793, 2905
NspI RCATGY 6 cut(s) 1485, 1847, 1894, 2118, 2763, 2907
PaeI GCATGC 2 cut(s) 1847, 2763
PagI TCATGA 2 cut(s) 31, 2485
PceI AGGCCT 1 cut(s) 1431
PciI ACATGT 3 cut(s) 1481, 1890, 2114
PctI GAATGC 1 cut(s) 658
PdiI GCCGGC 1 cut(s) 2360
PdmI GAANNNNTTC 1 cut(s) 2094
PfoI TCCNGGA 1 cut(s) 326
PkrI GCNGC 7 cut(s) 271, 294, 400, 403, 843, 931, 1941
Ple19I CGATCG 1 cut(s) 2042
PleI GAGTC 3 cut(s) 44, 197, 1580
PpsI GAGTC 3 cut(s) 44, 197, 1580
Ppu21I YACGTR 1 cut(s) 13
PscI ACATGT 3 cut(s) 1481, 1890, 2114
PshAI GACNNNNGTC 1 cut(s) 2710
PsiI TTATAA 1 cut(s) 1518
Psp6I CCWGG 5 cut(s) 192, 1081, 1130, 1287, 2933
PspEI GGTNACC 1 cut(s) 2929
PspGI CCWGG 5 cut(s) 192, 1081, 1130, 1287, 2933
PspN4I GGNNCC 4 cut(s) 444, 867, 2679, 2870
PspPI GGNCC 7 cut(s) 318, 866, 1085, 1814, 2146, 2496, 2821
PstI CTGCAG 2 cut(s) 843, 2569
PstNI CAGNNNCTG 2 cut(s) 629, 1877
PsuI RGATCY 2 cut(s) 2032, 2473
PvuI CGATCG 1 cut(s) 2042
RsaI GTAC 5 cut(s) 286, 858, 1024, 2828, 2870
RsaNI GTAC 5 cut(s) 285, 857, 1023, 2827, 2869
RseI CAYNNNNRTG 5 cut(s) 195, 576, 1160, 1229, 2908
SatI GCNGC 7 cut(s) 270, 293, 399, 402, 842, 930, 1940
Sau96I GGNCC 7 cut(s) 318, 866, 1085, 1814, 2146, 2496, 2821
SchI GAGTC 3 cut(s) 44, 197, 1581
ScrFI CCNGG 8 cut(s) 194, 242, 328, 519, 1083, 1132, 1289, 2935
SduI GDGCHC 5 cut(s) 1398, 2122, 2440, 2628, 2948
SfaNI GCATC 7 cut(s) 385, 684, 800, 1750, 2260, 2311, 2493
SfcI CTRYAG 3 cut(s) 839, 1260, 2565
SinI GGWCC 2 cut(s) 1085, 2496
SmiMI CAYNNNNRTG 5 cut(s) 195, 576, 1160, 1229, 2908
SmlI CTYRAG 6 cut(s) 1199, 1236, 1751, 1904, 1945, 2939
SmoI CTYRAG 6 cut(s) 1199, 1236, 1751, 1904, 1945, 2939
SphI GCATGC 2 cut(s) 1847, 2763
SseBI AGGCCT 1 cut(s) 1431
SsiI CCGC 6 cut(s) 227, 293, 297, 399, 844, 2728
SspMI CTAG 8 cut(s) 96, 618, 1433, 1493, 1545, 1631, 1787, 1838
StuI AGGCCT 1 cut(s) 1431
StyD4I CCNGG 8 cut(s) 192, 240, 326, 517, 1081, 1130, 1287, 2933
StyI CCWWGG 4 cut(s) 869, 1426, 1582, 1638
TaaI ACNGT 6 cut(s) 461, 816, 1208, 1357, 1606, 2831
TaiI ACGT 4 cut(s) 15, 556, 1794, 2710
TaqI TCGA 5 cut(s) 1189, 1212, 1758, 2311, 2808
TatI WGTACW 2 cut(s) 856, 1022
TauI GCSGC 2 cut(s) 295, 401
TscAI CASTG 4 cut(s) 1609, 2110, 2127, 2145
TseFI GTSAC 1 cut(s) 2929
TseI GCWGC 5 cut(s) 269, 401, 841, 929, 1939
Tsp45I GTSAC 1 cut(s) 2929
TspGWI ACGGA 1 cut(s) 1005
TspRI CASTG 4 cut(s) 1609, 2110, 2127, 2145
Vha464I CTTAAG 1 cut(s) 1199
VneI GTGCAC 2 cut(s) 1394, 2118
VpaK11BI GGWCC 2 cut(s) 1085, 2496
XapI RAATTY 8 cut(s) 357, 808, 998, 1436, 1488, 2053, 2093, 2210
XbaI TCTAGA 1 cut(s) 1786
XceI RCATGY 6 cut(s) 1485, 1847, 1894, 2118, 2763, 2907
XcmI CCANNNNNNNNNTGG 1 cut(s) 1372
XmnI GAANNNNTTC 1 cut(s) 2094
XspI CTAG 8 cut(s) 96, 618, 1433, 1493, 1545, 1631, 1787, 1838
Zsp2I ATGCAT 2 cut(s) 793, 2905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.