AT5G50330

aarF domain-containing protein kinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005306
Physical Location & Seq
Reverse (-)
20485134 .. 20488702
3569 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50330.2

Sequence Viewer

Length: 1440 bp
ATGCATGGGTTCGATTTCAAAGAGATCCAGGAGAAGATCTCCGATAGCTTTCGTCCATGGCAACGCTCTTTTCAATTCTGGGTTCGAGCTACTAATATCTACACAGGCTACAAGGTGTTTCAACTTCGAGTAAGTTTAGTAAAAGATGCAAAGAAGCAAGAGGAAATGTGGGAAAGACAACATGAACAAGCTGCTGATAAGATTTACTTCATGTGCTCTGATCTTGGTGGTTTCTTCCTTAAGATTGCTCAACTTTTAGCAAAACCTGACATGGCACCAGCTGCGTGGGTGAAAAAGCTTGTCACTTTGTGTGATCAAGCTCCTGCGACGCCGTTTGATGCGATTCAACTTGTTCTAGAGAAGGAGTTAGGTAAAAGCATTGGTGAAATCTTTGAAACGTTTGATGAAAAGCCTCTTGGTTCTGCTTCTATTGCTCAGGTTCATAGAGCAATAGTAAAGGGTAACAAGATGAATGTTGTTGTAAAGGTTCAACATCCTGGCATTGAAAGATTGATGATGACAGACATAAGAAACTTGCAACTATTCGCCTTATACATGCAGAGAACAGATATCAAATTTGACCTGCATTCCATAACAAAGGAAATGGAGAAACAGATTGGTTATGAATTTGACTTCAAAAGGGAGGCCAATGCAATGGAAAGAATTCGATGTTTTCTTTATGAGAACAACAAAAAGTCTCCTGTTTTGGTACCAAGAGTTCTGCGAGATATGGTGACTAAGAGGGTTTTGGTGATGGAATACATTAATGGAATTCCAATCCTGAGCATTGGTGATGAGATGGCTAAAAGAGGGATAAATCCTCATGGTAAGATTGCAGAGGCAGCAAAGCACAATATACTTAACAGTTTATCCCGTGCGTATGGCCAAATGATACTGAAAAGTGGTTTCTTTCATGCGGATCCACATCCTGGAAATATCTTGATTTGCAAAGGTCAAGAGGTTGCATTGCTGGACTATGGTCAAGTGAAGGAACTACCCAACAAGTTGAGACTTGGTTATGCAAATTTGGTCATTGCCATGGCTGATAACAATGCTTCACGAGTATCACAGAGCTTTTGGGAAATGGGTTTACATACAGTGGCCAAGTGTGAGAATGAGCAGCAAGAACTTCTGCGGTTAGCACAAACACTTTTTGATACAAAGATGCCTACTGGTCAGACAGTTTTACAACCTTTCTCAGATGACTCTTCGATTAAAAAGATAGCCGTTGAGACATTTCCAGAGGAACTGTTCTCTGTACTTCGCACTGTGGTTCTGTTAAGAGGACTCAGTGTTGGGATGGGAGTTAATTATTCGTGTGCAGAACAATGGAGAGCAATGGCCGAAGAGGCTTTACTTGCATCTGGAAGAGTGACAAGAGATGGCAAAGAAAGATCTCGTAGACGGGCTTCTCTACGAAGGCTACGCACAGGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

479

Amino Acids

54.63

Weight (kDa)

9.25

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 103 - 341 3e-50 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 591
Acc65I GGTACC 1 cut(s) 709
AccB1I GGYRCC 2 cut(s) 274, 709
AccB7I CCANNNNNTGG 1 cut(s) 929
AccI GTMKAC 1 cut(s) 1402
AciI CCGC 2 cut(s) 917, 1135
AclI AACGTT 1 cut(s) 398
AclWI GGATC 3 cut(s) 19, 914, 927
AcoI YGGCCR 3 cut(s) 883, 1101, 1341
AcsI RAATTY 5 cut(s) 575, 626, 663, 771, 1024
AcyI GRCGYC 1 cut(s) 329
AdeI CACNNNGTG 1 cut(s) 309
AfaI GTAC 2 cut(s) 711, 1260
AfiI CCNNNNNNNGG 1 cut(s) 929
AflII CTTAAG 1 cut(s) 239
AgsI TTSAA 8 cut(s) 19, 74, 122, 347, 395, 491, 506, 637
AjnI CCWGG 3 cut(s) 27, 496, 928
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 7 cut(s) 48, 89, 191, 281, 298, 320, 1074
AluI AGCT 7 cut(s) 48, 89, 191, 281, 298, 320, 1074
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 3 cut(s) 702, 1003, 1226
AlwI GGATC 3 cut(s) 19, 914, 927
AoxI GGCC 4 cut(s) 645, 883, 1101, 1341
ApeKI GCWGC 4 cut(s) 191, 281, 842, 1120
ApoI RAATTY 5 cut(s) 575, 626, 663, 771, 1024
AseI ATTAAT 1 cut(s) 765
Asp700I GAANNNNTTC 1 cut(s) 663
Asp718I GGTACC 1 cut(s) 709
AsuHPI GGTGA 5 cut(s) 301, 395, 745, 763, 803
BalI TGGCCA 2 cut(s) 885, 1103
BamHI GGATCC 1 cut(s) 919
BanI GGYRCC 2 cut(s) 274, 709
BarI GAAGNNNNNNTAC 4 cut(s) 1338, 1370, 1393, 1425
BauI CACGAG 1 cut(s) 1059
Bbv12I GWGCWC 1 cut(s) 218
BbvI GCAGC 4 cut(s) 178, 268, 854, 1132
BccI CCATC 4 cut(s) 748, 793, 1294, 1376
BceAI ACGGC 2 cut(s) 316, 1211
BciT130I CCWGG 3 cut(s) 29, 498, 930
BclI TGATCA 1 cut(s) 313
BcoDI GTCTC 3 cut(s) 702, 1003, 1226
BfaI CTAG 1 cut(s) 356
BfrI CTTAAG 1 cut(s) 239
BfuAI ACCTGC 1 cut(s) 591
BglI GCCNNNNNGGC 1 cut(s) 1349
BglII AGATCT 2 cut(s) 36, 1394
BisI GCNGC 4 cut(s) 192, 282, 843, 1121
BlsI GCNGC 4 cut(s) 193, 283, 844, 1122
Bme1390I CCNGG 3 cut(s) 29, 498, 930
BmiI GGNNCC 3 cut(s) 276, 711, 921
BmrFI CCNGG 3 cut(s) 29, 498, 930
BmsI GCATC 4 cut(s) 136, 328, 1155, 1370
BoxI GACNNNNGTC 1 cut(s) 978
BplI GAGNNNNNCTC 2 cut(s) 23, 55
Bpu10I CCTNAGC 2 cut(s) 435, 782
BsaBI GATNNNNATC 1 cut(s) 924
BsaHI GRCGYC 1 cut(s) 329
BsaJI CCNNGG 2 cut(s) 56, 1038
Bsc4I CCNNNNNNNGG 1 cut(s) 929
Bse1I ACTGG 1 cut(s) 1177
Bse3DI GCAATG 4 cut(s) 660, 965, 1032, 1344
Bse8I GATNNNNATC 1 cut(s) 924
BseBI CCWGG 3 cut(s) 29, 498, 930
BseDI CCNNGG 2 cut(s) 56, 1038
BseGI GGATG 3 cut(s) 493, 925, 1305
BseJI GATNNNNATC 1 cut(s) 924
BseLI CCNNNNNNNGG 1 cut(s) 929
BseMI GCAATG 4 cut(s) 660, 965, 1032, 1344
BseMII CTCAG 4 cut(s) 449, 773, 1212, 1303
BseNI ACTGG 1 cut(s) 1177
BseXI GCAGC 4 cut(s) 178, 268, 854, 1132
BsgI GTGCAG 1 cut(s) 1341
BshFI GGCC 4 cut(s) 647, 885, 1103, 1343
BshNI GGYRCC 2 cut(s) 274, 709
BsiHKAI GWGCWC 1 cut(s) 218
BslI CCNNNNNNNGG 1 cut(s) 929
BsmAI GTCTC 3 cut(s) 702, 1003, 1226
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 4 cut(s) 647, 885, 1103, 1343
Bsp1286I GDGCHC 1 cut(s) 218
Bsp143I GATC 6 cut(s) 24, 36, 220, 313, 919, 1394
Bsp19I CCATGG 2 cut(s) 56, 1038
BspACI CCGC 2 cut(s) 917, 1135
BspANI GGCC 4 cut(s) 647, 885, 1103, 1343
BspCNI CTCAG 4 cut(s) 448, 774, 1211, 1302
BspLI GGNNCC 3 cut(s) 276, 711, 921
BspMI ACCTGC 1 cut(s) 591
BspPI GGATC 3 cut(s) 19, 914, 927
BspT107I GGYRCC 2 cut(s) 274, 709
BspTI CTTAAG 1 cut(s) 239
BsrDI GCAATG 4 cut(s) 660, 965, 1032, 1344
BsrI ACTGG 1 cut(s) 1177
BssECI CCNNGG 2 cut(s) 56, 1038
BssMI GATC 6 cut(s) 24, 36, 220, 313, 919, 1394
BssNI GRCGYC 1 cut(s) 329
BssSI CACGAG 1 cut(s) 1059
BssT1I CCWWGG 2 cut(s) 56, 1038
Bst2BI CACGAG 1 cut(s) 1059
Bst2UI CCWGG 3 cut(s) 29, 498, 930
Bst4CI ACNGT 5 cut(s) 866, 1099, 1183, 1251, 1270
Bst6I CTCTTC 3 cut(s) 1213, 1341, 1363
BstACI GRCGYC 1 cut(s) 329
BstAFI CTTAAG 1 cut(s) 239
BstAPI GCANNNNNTGC 1 cut(s) 281
BstDEI CTNAG 5 cut(s) 435, 738, 782, 1198, 1289
BstDSI CCRYGG 2 cut(s) 56, 1038
BstF5I GGATG 3 cut(s) 493, 925, 1305
BstKTI GATC 6 cut(s) 27, 39, 223, 316, 922, 1397
BstMAI GTCTC 3 cut(s) 702, 1003, 1226
BstMBI GATC 6 cut(s) 24, 36, 220, 313, 919, 1394
BstMWI GCNNNNNNNGC 5 cut(s) 281, 431, 842, 1349, 1358
BstNI CCWGG 3 cut(s) 29, 498, 930
BstNSI RCATGY 1 cut(s) 559
BstPAI GACNNNNGTC 1 cut(s) 978
BstSCI CCNGG 3 cut(s) 27, 496, 928
BstV1I GCAGC 4 cut(s) 178, 268, 854, 1132
BstX2I RGATCY 4 cut(s) 24, 36, 919, 1394
BstXI CCANNNNNNTGG 2 cut(s) 285, 655
BstYI RGATCY 4 cut(s) 24, 36, 919, 1394
BsuRI GGCC 4 cut(s) 647, 885, 1103, 1343
BtgI CCRYGG 2 cut(s) 56, 1038
BtsCI GGATG 3 cut(s) 493, 925, 1305
BtsIMutI CAGTG 3 cut(s) 1104, 1266, 1297
BveI ACCTGC 1 cut(s) 591
CseI GACGC 1 cut(s) 337
Csp6I GTAC 2 cut(s) 710, 1259
CviAII CATG 9 cut(s) 5, 57, 182, 211, 271, 556, 824, 914, 1039
CviQI GTAC 2 cut(s) 710, 1259
DdeI CTNAG 5 cut(s) 435, 738, 782, 1198, 1289
DpnI GATC 6 cut(s) 26, 38, 222, 315, 921, 1396
DpnII GATC 6 cut(s) 24, 36, 220, 313, 919, 1394
DraIII CACNNNGTG 1 cut(s) 309
EaeI YGGCCR 3 cut(s) 883, 1101, 1341
Eam1104I CTCTTC 3 cut(s) 1213, 1341, 1363
EarI CTCTTC 3 cut(s) 1213, 1341, 1363
Eco130I CCWWGG 2 cut(s) 56, 1038
Eco32I GATATC 1 cut(s) 571
EcoRI GAATTC 2 cut(s) 663, 771
EcoRII CCWGG 3 cut(s) 27, 496, 928
EcoRV GATATC 1 cut(s) 571
EcoT14I CCWWGG 2 cut(s) 56, 1038
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 56, 1038
FaeI CATG 9 cut(s) 8, 60, 185, 214, 274, 559, 827, 917, 1042
FalI AAGNNNNNCTT 2 cut(s) 191, 223
FatI CATG 9 cut(s) 4, 56, 181, 210, 270, 555, 823, 913, 1038
FbaI TGATCA 1 cut(s) 313
FblI GTMKAC 1 cut(s) 1402
Fnu4HI GCNGC 4 cut(s) 192, 282, 843, 1121
FokI GGATG 3 cut(s) 480, 912, 1312
Fsp4HI GCNGC 4 cut(s) 192, 282, 843, 1121
FspBI CTAG 1 cut(s) 356
GluI GCNGC 4 cut(s) 192, 282, 843, 1121
HaeIII GGCC 4 cut(s) 647, 885, 1103, 1343
HgaI GACGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 329
Hin1II CATG 9 cut(s) 8, 60, 185, 214, 274, 559, 827, 917, 1042
HindIII AAGCTT 1 cut(s) 296
HinfI GANTC 3 cut(s) 343, 1205, 1287
HphI GGTGA 5 cut(s) 301, 395, 745, 763, 803
Hpy166II GTNNAC 2 cut(s) 1091, 1403
Hpy188I TCNGA 4 cut(s) 43, 220, 1179, 1201
Hpy188III TCNNGA 7 cut(s) 356, 781, 940, 956, 1059, 1241, 1365
Hpy8I GTNNAC 2 cut(s) 1091, 1403
Hpy99I CGWCG 1 cut(s) 331
HpyAV CCTTC 3 cut(s) 355, 982, 1413
HpyCH4III ACNGT 5 cut(s) 866, 1099, 1183, 1251, 1270
HpyCH4IV ACGT 1 cut(s) 398
HpyF10VI GCNNNNNNNGC 5 cut(s) 281, 431, 842, 1349, 1358
HpyF3I CTNAG 5 cut(s) 435, 738, 782, 1198, 1289
HpySE526I ACGT 1 cut(s) 398
Hsp92I GRCGYC 1 cut(s) 329
Hsp92II CATG 9 cut(s) 8, 60, 185, 214, 274, 559, 827, 917, 1042
KpnI GGTACC 1 cut(s) 713
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 6 cut(s) 24, 36, 220, 313, 919, 1394
LmnI GCTCC 1 cut(s) 325
Lsp1109I GCAGC 4 cut(s) 178, 268, 854, 1132
LweI GCATC 4 cut(s) 136, 328, 1155, 1370
MaeI CTAG 1 cut(s) 356
MaeII ACGT 1 cut(s) 398
MaeIII GTNAC 4 cut(s) 301, 461, 733, 1372
MalI GATC 6 cut(s) 26, 38, 222, 315, 921, 1396
MboI GATC 6 cut(s) 24, 36, 220, 313, 919, 1394
MboII GAAGA 5 cut(s) 46, 226, 1200, 1358, 1380
MflI RGATCY 4 cut(s) 24, 36, 919, 1394
MhlI GDGCHC 1 cut(s) 218
MlsI TGGCCA 2 cut(s) 885, 1103
MluCI AATT 7 cut(s) 74, 575, 626, 663, 771, 1024, 1309
MluNI TGGCCA 2 cut(s) 885, 1103
MlyI GAGTC 2 cut(s) 1199, 1281
Mox20I TGGCCA 2 cut(s) 885, 1103
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 663
MscI TGGCCA 2 cut(s) 885, 1103
MseI TTAA 6 cut(s) 240, 765, 861, 1215, 1280, 1308
MslI CAYNNNNRTG 1 cut(s) 1037
Msp20I TGGCCA 2 cut(s) 885, 1103
MspA1I CMGCKG 1 cut(s) 281
MspCI CTTAAG 1 cut(s) 239
MspR9I CCNGG 3 cut(s) 29, 498, 930
Mva1269I GAATGC 1 cut(s) 586
MvaI CCWGG 3 cut(s) 29, 498, 930
MwoI GCNNNNNNNGC 5 cut(s) 281, 431, 842, 1349, 1358
NcoI CCATGG 2 cut(s) 56, 1038
NdeII GATC 6 cut(s) 24, 36, 220, 313, 919, 1394
NlaIII CATG 9 cut(s) 8, 60, 185, 214, 274, 559, 827, 917, 1042
NlaIV GGNNCC 3 cut(s) 276, 711, 921
NmuCI GTSAC 3 cut(s) 301, 733, 1372
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 559
PctI GAATGC 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 663
PfeI GAWTC 1 cut(s) 343
PflMI CCANNNNNTGG 1 cut(s) 929
PfoI TCCNGGA 2 cut(s) 27, 928
PkrI GCNGC 4 cut(s) 193, 283, 844, 1122
PleI GAGTC 2 cut(s) 1199, 1281
PpsI GAGTC 2 cut(s) 1199, 1281
PshAI GACNNNNGTC 1 cut(s) 978
PshBI ATTAAT 1 cut(s) 765
Psp1406I AACGTT 1 cut(s) 398
Psp6I CCWGG 3 cut(s) 27, 496, 928
PspGI CCWGG 3 cut(s) 27, 496, 928
PspN4I GGNNCC 3 cut(s) 276, 711, 921
PsrI GAACNNNNNNTAC 2 cut(s) 702, 734
PsuI RGATCY 4 cut(s) 24, 36, 919, 1394
PvuII CAGCTG 1 cut(s) 281
RsaI GTAC 2 cut(s) 711, 1260
RsaNI GTAC 2 cut(s) 710, 1259
RseI CAYNNNNRTG 1 cut(s) 1037
SaqAI TTAA 6 cut(s) 240, 765, 861, 1215, 1280, 1308
SatI GCNGC 4 cut(s) 192, 282, 843, 1121
Sau3AI GATC 6 cut(s) 24, 36, 220, 313, 919, 1394
SchI GAGTC 2 cut(s) 1199, 1281
ScrFI CCNGG 3 cut(s) 29, 498, 930
SduI GDGCHC 1 cut(s) 218
SfaNI GCATC 4 cut(s) 136, 328, 1155, 1370
SmiMI CAYNNNNRTG 1 cut(s) 1037
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 7 cut(s) 74, 575, 626, 663, 771, 1024, 1309
SsiI CCGC 2 cut(s) 917, 1135
SspMI CTAG 1 cut(s) 356
StyD4I CCNGG 3 cut(s) 27, 496, 928
StyI CCWWGG 2 cut(s) 56, 1038
TaaI ACNGT 5 cut(s) 866, 1099, 1183, 1251, 1270
TaiI ACGT 1 cut(s) 401
TaqI TCGA 5 cut(s) 12, 85, 127, 667, 1211
TasI AATT 7 cut(s) 74, 575, 626, 663, 771, 1024, 1309
TatI WGTACW 1 cut(s) 1258
TfiI GAWTC 1 cut(s) 343
Tru1I TTAA 6 cut(s) 240, 765, 861, 1215, 1280, 1308
Tru9I TTAA 6 cut(s) 240, 765, 861, 1215, 1280, 1308
TscAI CASTG 3 cut(s) 1104, 1273, 1297
TseFI GTSAC 3 cut(s) 301, 733, 1372
TseI GCWGC 4 cut(s) 191, 281, 842, 1120
Tsp45I GTSAC 3 cut(s) 301, 733, 1372
TspDTI ATGAA 7 cut(s) 198, 199, 420, 431, 485, 639, 902
TspRI CASTG 3 cut(s) 1104, 1273, 1297
Van91I CCANNNNNTGG 1 cut(s) 929
Vha464I CTTAAG 1 cut(s) 239
VspI ATTAAT 1 cut(s) 765
XapI RAATTY 5 cut(s) 575, 626, 663, 771, 1024
XbaI TCTAGA 1 cut(s) 355
XceI RCATGY 1 cut(s) 559
XmiI GTMKAC 1 cut(s) 1402
XmnI GAANNNNTTC 1 cut(s) 663
XspI CTAG 1 cut(s) 356
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.