pycom06g13850

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005306
Physical Location & Seq
Reverse (-)
18723184 .. 18727453
4270 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g13850.1

Sequence Viewer

Length: 1449 bp
ATGCTTCCTCTGCCTCCCATTGATCTCAAAGATGTTCAGCAGAAGCTCGCCACCCATTTCAGGCCATGGCAGCGTTCCTTTCAGTTCTGGGTTCGAGCTGTTGATATCTACACTGGCTACAAGGCGTTTCAGCTGAGAGTGAGTTTGGTGAAGGATGCGAAGAAGCAAGAGGAAATGTGGGAGAGGCAGCATGAATTTGCAGCTGAGAAGATATATGCAATGTGCTCGGACCTTGGCGGTTTCTTCCTCAAGGTTGCCCAAATTGTCGGGAAGCCAGACTTGGCCCCAGCTGCATGGGTGAAAAGGCTTGTTACATTGTGTGATCACGCTCCTGCAACCCCATTTGATGCTGTGCAACTTATGTTGGAGATGGAGTTGGGTCGAAGTGTTGGCGAAGTGTTTGAAAGATTCGATGTGGATCCTCTTGGCTCTGCCTCAATTGCACAGGTTCACCGAGCAAGATTGAGAGGTGATAAAACTGATGTTGTTGTCAAGGTGCAACATCCTGGAGTTCAGGACCTAATGATGACAGATATCCATAACGTCCAAGCCTTTGCTTTATACATGCAAAAGACAGATATCAAATTTGATCTATACTCTGTAACTAAGGAAATGGAGAAACAGATTGGATACGAATTTGATTTCGTGAGGGAGGCTGATGCTATGGAAAAGATTAGACGTTTTTTGTATGAGAATAACAAAAGGAGTCCTGTTTTGGTGCCACGACTTATACGTGATATGGTCACTAGGAGAGTCTTAGTTATGGAATATATTGATGGAACCCCAATCCTGAACCTCGGTGATGAAATTGCGAAAAGAGGCATAAACCCTGGTGGTAAGATTGCAGCAGCCGCAAAGAAGAAAATCCTTCAAAGCTTGACGCTAGCATATGGACAAATGATTTTGAAGACTGGTTTCTTCCATGCAGATCCTCATCCAGGAAATATTCTAATCTGTAAAGGCTCGGAGGTTGCCTTGCTGGACTATGGCCAAGTGAAAGATCTACCCAATGAGTTGAGACTTGGTTATGCTAACCTTGTTCTTGCCATTGCCGACGGTGACCCTATTAGAGCATCAGAGAGCTACAGGGAGCTTGGCATAGAAACCTTAAGCAAATGTGAAAATGAACAGCATGAAATGTTGAAGTTGGCACAGACAATGTTTGACACAAAATTACCCCCCGGAGTGACGATGCTGCAACCTTTTTCAGACGAATCTTCAATTAAAAAAATTGCGGTTCAGGGTTTTCCAGAGGAGTTGTTTTCTGTACTTCGGACAGTGCATCTCCTGAGAGGACTTAGCGTCGGTTTGGGAATCAACTACTCATGTGCGGAACAATGGAGACCAATTGCAGAAGAAGCTTTGTTCCGTGCTGGGAGATTAAAAGGTAGGCCTATCAAAGCCAGAGGTCGTAGGCGCGGTTTATCGAGGTTATTCAGGAGAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

483

Amino Acids

54.7

Weight (kDa)

9.1

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 106 - 357 3.3e-61 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 718
AccII CGCG 1 cut(s) 1419
AciI CCGC 5 cut(s) 237, 852, 1235, 1331, 1419
AclWI GGATC 3 cut(s) 413, 426, 923
AcoI YGGCCR 1 cut(s) 988
AcsI RAATTY 3 cut(s) 194, 584, 635
AfaI GTAC 1 cut(s) 1269
AfiI CCNNNNNNNGG 2 cut(s) 60, 938
AflII CTTAAG 1 cut(s) 1108
AgsI TTSAA 5 cut(s) 404, 872, 907, 1144, 1221
AhdI GACNNNNNGTC 1 cut(s) 1301
AjnI CCWGG 3 cut(s) 505, 829, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 9 cut(s) 46, 98, 133, 203, 290, 876, 1083, 1093, 1361
AluI AGCT 9 cut(s) 46, 98, 133, 203, 290, 876, 1083, 1093, 1361
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 3 cut(s) 1012, 1336, 1437
AlwI GGATC 3 cut(s) 413, 426, 923
AoxI GGCC 4 cut(s) 62, 282, 988, 1391
ApeKI GCWGC 7 cut(s) 70, 187, 200, 290, 845, 848, 1195
ApoI RAATTY 3 cut(s) 194, 584, 635
AspLEI GCGC 1 cut(s) 1419
AspS9I GGNCC 3 cut(s) 229, 283, 517
AsuC2I CCSGG 1 cut(s) 1182
AsuHPI GGTGA 6 cut(s) 160, 310, 443, 482, 812, 1070
AsuNHI GCTAGC 1 cut(s) 883
AvaII GGWCC 2 cut(s) 229, 517
BalI TGGCCA 1 cut(s) 990
BamHI GGATCC 1 cut(s) 418
BanI GGYRCC 1 cut(s) 718
BbsI GAAGAC 1 cut(s) 914
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 7 cut(s) 82, 199, 212, 277, 857, 860, 1182
BccI CCATC 2 cut(s) 364, 770
BcgI CGANNNNNNTGC 2 cut(s) 207, 241
BciT130I CCWGG 3 cut(s) 507, 831, 939
BciVI GTATCC 1 cut(s) 623
BclI TGATCA 1 cut(s) 322
BcnI CCSGG 1 cut(s) 1182
BcoDI GTCTC 3 cut(s) 1012, 1336, 1437
BfaI CTAG 2 cut(s) 747, 884
BfmI CTRYAG 1 cut(s) 1084
BfrI CTTAAG 1 cut(s) 1108
BfuI GTATCC 1 cut(s) 623
BglII AGATCT 1 cut(s) 1000
BisI GCNGC 8 cut(s) 71, 188, 201, 291, 846, 849, 852, 1196
BlsI GCNGC 8 cut(s) 72, 189, 202, 292, 847, 850, 853, 1197
Bme1390I CCNGG 4 cut(s) 507, 831, 939, 1182
Bme18I GGWCC 2 cut(s) 229, 517
BmeRI GACNNNNNGTC 1 cut(s) 1301
BmgT120I GGNCC 3 cut(s) 229, 283, 517
BmiI GGNNCC 4 cut(s) 285, 420, 720, 781
BmrFI CCNGG 4 cut(s) 507, 831, 939, 1182
BmsI GCATC 6 cut(s) 145, 337, 649, 1082, 1182, 1291
BmtI GCTAGC 1 cut(s) 887
BpiI GAAGAC 1 cut(s) 914
BpmI CTGGAG 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 233
BpuMI CCSGG 1 cut(s) 1182
BsaAI YACGTR 1 cut(s) 734
BsaBI GATNNNNATC 2 cut(s) 417, 933
BsaI GGTCTC 1 cut(s) 1336
BsaJI CCNNGG 5 cut(s) 65, 232, 796, 829, 1180
BsaXI ACNNNNNCTCC 2 cut(s) 359, 389
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 938
Bse1I ACTGG 2 cut(s) 118, 916
Bse3DI GCAATG 2 cut(s) 225, 1047
Bse8I GATNNNNATC 2 cut(s) 417, 933
BseBI CCWGG 3 cut(s) 507, 831, 939
BseDI CCNNGG 5 cut(s) 65, 232, 796, 829, 1180
BseGI GGATG 3 cut(s) 160, 502, 934
BseJI GATNNNNATC 2 cut(s) 417, 933
BseLI CCNNNNNNNGG 2 cut(s) 60, 938
BseMI GCAATG 2 cut(s) 225, 1047
BseMII CTCAG 3 cut(s) 125, 195, 1280
BseNI ACTGG 2 cut(s) 118, 916
BseRI GAGGAG 1 cut(s) 1268
BseXI GCAGC 7 cut(s) 82, 199, 212, 277, 857, 860, 1182
BseYI CCCAGC 2 cut(s) 286, 1373
Bsh1236I CGCG 1 cut(s) 1419
BshFI GGCC 4 cut(s) 64, 284, 990, 1393
BshNI GGYRCC 1 cut(s) 718
BsiHKAI GWGCWC 1 cut(s) 227
BsiSI CCGG 1 cut(s) 1182
BslI CCNNNNNNNGG 2 cut(s) 60, 938
BsmAI GTCTC 3 cut(s) 1012, 1336, 1437
BsnI GGCC 4 cut(s) 64, 284, 990, 1393
Bso31I GGTCTC 1 cut(s) 1336
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 6 cut(s) 22, 322, 418, 589, 928, 1000
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 5 cut(s) 237, 852, 1235, 1331, 1419
BspANI GGCC 4 cut(s) 64, 284, 990, 1393
BspCNI CTCAG 3 cut(s) 126, 196, 1281
BspFNI CGCG 1 cut(s) 1419
BspLI GGNNCC 4 cut(s) 285, 420, 720, 781
BspOI GCTAGC 1 cut(s) 887
BspPI GGATC 3 cut(s) 413, 426, 923
BspT107I GGYRCC 1 cut(s) 718
BspTI CTTAAG 1 cut(s) 1108
BspTNI GGTCTC 1 cut(s) 1336
BsrDI GCAATG 2 cut(s) 225, 1047
BsrI ACTGG 2 cut(s) 118, 916
BssECI CCNNGG 5 cut(s) 65, 232, 796, 829, 1180
BssMI GATC 6 cut(s) 22, 322, 418, 589, 928, 1000
BssT1I CCWWGG 2 cut(s) 65, 232
Bst2UI CCWGG 3 cut(s) 507, 831, 939
Bst4CI ACNGT 2 cut(s) 1058, 1279
BstAFI CTTAAG 1 cut(s) 1108
BstBAI YACGTR 1 cut(s) 734
BstC8I GCNNGC 2 cut(s) 48, 885
BstDEI CTNAG 6 cut(s) 134, 204, 606, 757, 1289, 1298
BstDSI CCRYGG 1 cut(s) 65
BstEII GGTNACC 1 cut(s) 1058
BstENI CCTNNNNNAGG 1 cut(s) 936
BstF5I GGATG 3 cut(s) 160, 502, 934
BstFNI CGCG 1 cut(s) 1419
BstHHI GCGC 1 cut(s) 1419
BstKTI GATC 6 cut(s) 25, 325, 421, 592, 931, 1003
BstMAI GTCTC 3 cut(s) 1012, 1336, 1437
BstMBI GATC 6 cut(s) 22, 322, 418, 589, 928, 1000
BstMWI GCNNNNNNNGC 6 cut(s) 10, 70, 290, 440, 851, 1358
BstNI CCWGG 3 cut(s) 507, 831, 939
BstNSI RCATGY 1 cut(s) 568
BstPI GGTNACC 1 cut(s) 1058
BstSCI CCNGG 4 cut(s) 505, 829, 937, 1180
BstSFI CTRYAG 1 cut(s) 1084
BstUI CGCG 1 cut(s) 1419
BstV1I GCAGC 7 cut(s) 82, 199, 212, 277, 857, 860, 1182
BstV2I GAAGAC 1 cut(s) 914
BstX2I RGATCY 3 cut(s) 418, 928, 1000
BstXI CCANNNNNNTGG 1 cut(s) 294
BstYI RGATCY 3 cut(s) 418, 928, 1000
BsuI GTATCC 1 cut(s) 623
BsuRI GGCC 4 cut(s) 64, 284, 990, 1393
BtgI CCRYGG 1 cut(s) 65
BtsCI GGATG 3 cut(s) 160, 502, 934
BtsIMutI CAGTG 2 cut(s) 111, 1284
Cac8I GCNNGC 2 cut(s) 48, 885
CfoI GCGC 1 cut(s) 1419
Cfr13I GGNCC 3 cut(s) 229, 283, 517
CseI GACGC 2 cut(s) 889, 1291
Csp6I GTAC 1 cut(s) 1268
CviAII CATG 7 cut(s) 66, 191, 294, 565, 923, 1133, 1326
CviQI GTAC 1 cut(s) 1268
DdeI CTNAG 6 cut(s) 134, 204, 606, 757, 1289, 1298
DpnI GATC 6 cut(s) 24, 324, 420, 591, 930, 1002
DpnII GATC 6 cut(s) 22, 322, 418, 589, 928, 1000
DriI GACNNNNNGTC 1 cut(s) 1301
EaeI YGGCCR 1 cut(s) 988
Eam1105I GACNNNNNGTC 1 cut(s) 1301
Eco130I CCWWGG 2 cut(s) 65, 232
Eco147I AGGCCT 1 cut(s) 1393
Eco31I GGTCTC 1 cut(s) 1336
Eco32I GATATC 3 cut(s) 106, 535, 580
Eco47I GGWCC 2 cut(s) 229, 517
Eco91I GGTNACC 1 cut(s) 1058
EcoNI CCTNNNNNAGG 1 cut(s) 936
EcoO109I RGGNCCY 1 cut(s) 517
EcoO65I GGTNACC 1 cut(s) 1058
EcoRII CCWGG 3 cut(s) 505, 829, 937
EcoRV GATATC 3 cut(s) 106, 535, 580
EcoT14I CCWWGG 2 cut(s) 65, 232
ErhI CCWWGG 2 cut(s) 65, 232
FaeI CATG 7 cut(s) 69, 194, 297, 568, 926, 1136, 1329
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FatI CATG 7 cut(s) 65, 190, 293, 564, 922, 1132, 1325
FauNDI CATATG 1 cut(s) 889
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 8 cut(s) 71, 188, 201, 291, 846, 849, 852, 1196
FokI GGATG 3 cut(s) 167, 489, 921
Fsp4HI GCNGC 8 cut(s) 71, 188, 201, 291, 846, 849, 852, 1196
FspBI CTAG 2 cut(s) 747, 884
GlaI GCGC 1 cut(s) 1418
GluI GCNGC 8 cut(s) 71, 188, 201, 291, 846, 849, 852, 1196
GsaI CCCAGC 2 cut(s) 290, 1377
GsuI CTGGAG 1 cut(s) 528
HaeIII GGCC 4 cut(s) 64, 284, 990, 1393
HapII CCGG 1 cut(s) 1182
HgaI GACGC 2 cut(s) 889, 1291
HhaI GCGC 1 cut(s) 1419
Hin1II CATG 7 cut(s) 69, 194, 297, 568, 926, 1136, 1329
Hin6I GCGC 1 cut(s) 1417
HinP1I GCGC 1 cut(s) 1417
HindIII AAGCTT 2 cut(s) 874, 1359
HinfI GANTC 5 cut(s) 408, 706, 753, 1214, 1314
HpaII CCGG 1 cut(s) 1182
HphI GGTGA 6 cut(s) 160, 310, 443, 482, 812, 1070
Hpy166II GTNNAC 1 cut(s) 451
Hpy188I TCNGA 5 cut(s) 229, 967, 1078, 1210, 1275
Hpy188III TCNNGA 7 cut(s) 268, 515, 646, 790, 1250, 1288, 1438
Hpy8I GTNNAC 1 cut(s) 451
Hpy99I CGWCG 2 cut(s) 1058, 1307
HpyAV CCTTC 2 cut(s) 145, 878
HpyCH4III ACNGT 2 cut(s) 1058, 1279
HpyCH4IV ACGT 3 cut(s) 543, 679, 733
HpyF10VI GCNNNNNNNGC 6 cut(s) 10, 70, 290, 440, 851, 1358
HpyF3I CTNAG 6 cut(s) 134, 204, 606, 757, 1289, 1298
HpySE526I ACGT 3 cut(s) 543, 679, 733
Hsp92II CATG 7 cut(s) 69, 194, 297, 568, 926, 1136, 1329
HspAI GCGC 1 cut(s) 1417
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 6 cut(s) 22, 322, 418, 589, 928, 1000
LmnI GCTCC 2 cut(s) 334, 1090
Lsp1109I GCAGC 7 cut(s) 82, 199, 212, 277, 857, 860, 1182
LweI GCATC 6 cut(s) 145, 337, 649, 1082, 1182, 1291
MaeI CTAG 2 cut(s) 747, 884
MaeII ACGT 3 cut(s) 543, 679, 733
MaeIII GTNAC 5 cut(s) 310, 601, 742, 1058, 1186
MalI GATC 6 cut(s) 24, 324, 420, 591, 930, 1002
MboI GATC 6 cut(s) 22, 322, 418, 589, 928, 1000
MboII GAAGA 8 cut(s) 172, 220, 235, 871, 910, 919, 1209, 1367
MfeI CAATTG 2 cut(s) 438, 1347
MflI RGATCY 3 cut(s) 418, 928, 1000
MhlI GDGCHC 1 cut(s) 227
MlsI TGGCCA 1 cut(s) 990
MluNI TGGCCA 1 cut(s) 990
MlyI GAGTC 2 cut(s) 715, 762
MmeI TCCRAC 1 cut(s) 345
Mox20I TGGCCA 1 cut(s) 990
MscI TGGCCA 1 cut(s) 990
MseI TTAA 3 cut(s) 1109, 1224, 1382
Msp20I TGGCCA 1 cut(s) 990
MspA1I CMGCKG 3 cut(s) 133, 203, 290
MspCI CTTAAG 1 cut(s) 1108
MspI CCGG 1 cut(s) 1182
MspR9I CCNGG 4 cut(s) 507, 831, 939, 1182
MunI CAATTG 2 cut(s) 438, 1347
MvaI CCWGG 3 cut(s) 507, 831, 939
MvnI CGCG 1 cut(s) 1419
MwoI GCNNNNNNNGC 6 cut(s) 10, 70, 290, 440, 851, 1358
NciI CCSGG 1 cut(s) 1182
NcoI CCATGG 1 cut(s) 65
NdeI CATATG 1 cut(s) 889
NdeII GATC 6 cut(s) 22, 322, 418, 589, 928, 1000
NheI GCTAGC 1 cut(s) 883
NlaIII CATG 7 cut(s) 69, 194, 297, 568, 926, 1136, 1329
NlaIV GGNNCC 4 cut(s) 285, 420, 720, 781
NmuCI GTSAC 3 cut(s) 742, 1058, 1186
NspI RCATGY 1 cut(s) 568
PceI AGGCCT 1 cut(s) 1393
PcsI WCGNNNNNNNCGW 1 cut(s) 730
PfeI GAWTC 3 cut(s) 408, 1214, 1314
PfoI TCCNGGA 2 cut(s) 505, 937
PkrI GCNGC 8 cut(s) 72, 189, 202, 292, 847, 850, 853, 1197
PleI GAGTC 2 cut(s) 714, 761
PpsI GAGTC 2 cut(s) 714, 761
Ppu21I YACGTR 1 cut(s) 734
PpuMI RGGWCCY 1 cut(s) 517
Psp5II RGGWCCY 1 cut(s) 517
Psp6I CCWGG 3 cut(s) 505, 829, 937
PspEI GGTNACC 1 cut(s) 1058
PspFI CCCAGC 2 cut(s) 286, 1373
PspGI CCWGG 3 cut(s) 505, 829, 937
PspN4I GGNNCC 4 cut(s) 285, 420, 720, 781
PspPI GGNCC 3 cut(s) 229, 283, 517
PspPPI RGGWCCY 1 cut(s) 517
PsuI RGATCY 3 cut(s) 418, 928, 1000
PvuII CAGCTG 3 cut(s) 133, 203, 290
RsaI GTAC 1 cut(s) 1269
RsaNI GTAC 1 cut(s) 1268
SaqAI TTAA 3 cut(s) 1109, 1224, 1382
SatI GCNGC 8 cut(s) 71, 188, 201, 291, 846, 849, 852, 1196
Sau3AI GATC 6 cut(s) 22, 322, 418, 589, 928, 1000
Sau96I GGNCC 3 cut(s) 229, 283, 517
SchI GAGTC 2 cut(s) 715, 762
ScrFI CCNGG 4 cut(s) 507, 831, 939, 1182
SduI GDGCHC 1 cut(s) 227
SfaNI GCATC 6 cut(s) 145, 337, 649, 1082, 1182, 1291
SfcI CTRYAG 1 cut(s) 1084
SinI GGWCC 2 cut(s) 229, 517
SmlI CTYRAG 2 cut(s) 248, 1108
SmoI CTYRAG 2 cut(s) 248, 1108
SseBI AGGCCT 1 cut(s) 1393
SsiI CCGC 5 cut(s) 237, 852, 1235, 1331, 1419
SspI AATATT 1 cut(s) 946
SspMI CTAG 2 cut(s) 747, 884
StuI AGGCCT 1 cut(s) 1393
StyD4I CCNGG 4 cut(s) 505, 829, 937, 1180
StyI CCWWGG 2 cut(s) 65, 232
TaaI ACNGT 2 cut(s) 1058, 1279
TaiI ACGT 3 cut(s) 546, 682, 736
TaqI TCGA 4 cut(s) 94, 382, 411, 1427
TatI WGTACW 1 cut(s) 1267
TauI GCSGC 1 cut(s) 854
TfiI GAWTC 3 cut(s) 408, 1214, 1314
Tru1I TTAA 3 cut(s) 1109, 1224, 1382
Tru9I TTAA 3 cut(s) 1109, 1224, 1382
TscAI CASTG 2 cut(s) 118, 1284
TseFI GTSAC 3 cut(s) 742, 1058, 1186
TseI GCWGC 7 cut(s) 70, 187, 200, 290, 845, 848, 1195
Tsp45I GTSAC 3 cut(s) 742, 1058, 1186
TspDTI ATGAA 4 cut(s) 207, 819, 1140, 1149
TspGWI ACGGA 1 cut(s) 1358
TspRI CASTG 2 cut(s) 118, 1284
Vha464I CTTAAG 1 cut(s) 1108
VpaK11BI GGWCC 2 cut(s) 229, 517
XagI CCTNNNNNAGG 1 cut(s) 936
XapI RAATTY 3 cut(s) 194, 584, 635
XceI RCATGY 1 cut(s) 568
XspI CTAG 2 cut(s) 747, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.