Rh7BG117100

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0005306
Physical Location & Seq
Reverse (-)
8742715 .. 8746730
4016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG117100.1

Sequence Viewer

Length: 1530 bp
ATGTGTGCAGAAGACTCTATAAAATCCAATACAACAGAGAAGAAAGAAAATCCAGAACCCAATTCAGCAACGATAACAACTATGCTTCCTCTGCCTCCTATTGATCTCAAAGATGTTCGGCAGAAGCTGGCCACCCAATTCAGGCCTTGGGAACGCTCCTTTCAGTTCTGGGCTCGAGCCGTTGATATCTACACCGGCTACAAGGTGTTTCAGCTGCGAGTGAGTTTTGTGAAGGATGCCAAGAAACAAGAGGAAATGTGGGAGAGGCAACATGAAGTTGCGGCCGACAAGATATATGCTATGTGTTCGGACATGGGCGGTTTCTTCCTCAAGGTTGCCCAAATTGTTGGGAAGCCAGACTTGGCCCCAGCTGCATGGGTGAAAAGGCTTGTTACATTGTGTGATCATGCTCCTGCAACACCATTTGATGCCGTTCAACTTATGCTGGAAACTGAGTTGGGTCGAAGTGTTGGCGAACTGTTTGAGAGATTCGATGTGGATCCTATTGGCTCTGCTTCGATTGCACAGGTTCACCGAGCAAGATTGAGAGGTGATAAGAGTGATATTGTTGTCAAGGTGCAACATCCTGGAGTTCAGGATCTAATGATGACAGATATCCGTAACTTGCAAGCCTTTGCTTTATACATTCAAAAGACAGATGTCAAATTTGATCTATACTCCGTAACCAAGGAAATGGAGACACAGATTGGTTATGAATTCGACTTCATGAGGGAAGCTAATGCTATGGAAAAAATTCGAAAGTTTTTGTATGAGAATAACAAAAAGACTCCTGTTGTGGTTCCTCGACTGATACGAGACCTCGTCACTAGGAGAGTCTTAGTTATGGAATATATTGATGGAATACCAATCCTGAATCTTGGTGATGAAATTGCGAAAAGGGGTATAAATCCTAGTGGTGAGGTTGCAGCCGCGGCAAAGCAGAAAATCCTCCAAAGTTTGACACTAGCATATGGACAAATGATTCTGAAGAATGGTTTCTTCCATGCAGATCCCCACCCAGGAAATATCCTAATCTGTAAAGGTTCAGAGGTTGCGTTGCTTGACTACGGCCAAGTGAAAGATCTCCCAGATGAGTTGAGACTTGGTTATGCAAATCTTGTTCTTGCAATAGCTGATGGTGACCCTGTAAGAGCCTTGGAGAGCTATAGGGAGCTTGGCATAGAAACCTTGAGCACGTGTGAAAATGAACAGAAGGAAATGTTGAAGTTGGCCGAGACAATGTTTGACACGAAATTACCCCCTGGAGTGACGATGTTGCAGCCTTTTTCGGAGGAATCTTCTATTAAAAAGATTGCAGTTCAGGCTTTTCCAGAGGAGTTGTTTTCTGTACTTCGAACAGTGCATCTGCTGAGAGGGCTTAGTGTTGGACTGGGAATCAACTACTCATGTGCAGAACAATGGAGACCCATTGCAGAAGAAGCTCTGTTTCGTGCTGGGAGATTAAAAGGTAAAGATATCAAGGGTAGAAGTCGTAGAGGTGGTTTATTAAGGTTACTCTGGAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

509

Amino Acids

57.53

Weight (kDa)

7.59

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 133 - 386 6.8e-62 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 932
AciI CCGC 4 cut(s) 281, 318, 930, 932
AclWI GGATC 4 cut(s) 494, 507, 606, 1004
AcoI YGGCCR 4 cut(s) 129, 282, 1069, 1230
AcsI RAATTY 3 cut(s) 665, 716, 753
AcuI CTGAAG 1 cut(s) 1007
AcvI CACGTG 1 cut(s) 1197
AfaI GTAC 1 cut(s) 1350
AfiI CCNNNNNNNGG 2 cut(s) 141, 1019
AflIII ACRYGT 1 cut(s) 1196
AgsI TTSAA 3 cut(s) 437, 650, 1225
AjnI CCWGG 3 cut(s) 586, 1018, 1261
AjuI GAANNNNNNNTTGG 2 cut(s) 344, 376
AluBI AGCT 8 cut(s) 127, 214, 371, 737, 1133, 1164, 1174, 1442
AluI AGCT 8 cut(s) 127, 214, 371, 737, 1133, 1164, 1174, 1442
Alw21I GWGCWC 1 cut(s) 1196
Alw26I GTCTC 5 cut(s) 692, 810, 1093, 1229, 1417
AlwI GGATC 4 cut(s) 494, 507, 606, 1004
AlwNI CAGNNNCTG 1 cut(s) 127
Ama87I CYCGRG 1 cut(s) 174
AoxI GGCC 6 cut(s) 129, 143, 282, 363, 1069, 1230
ApeKI GCWGC 4 cut(s) 214, 371, 926, 1279
ApoI RAATTY 3 cut(s) 665, 716, 753
Asp700I GAANNNNTTC 2 cut(s) 753, 995
AspS9I GGNCC 1 cut(s) 364
AsuHPI GGTGA 6 cut(s) 391, 524, 563, 893, 929, 1151
AsuII TTCGAA 2 cut(s) 757, 1354
AvaI CYCGRG 1 cut(s) 174
BalI TGGCCA 1 cut(s) 131
BamHI GGATCC 1 cut(s) 499
BanII GRGCYC 1 cut(s) 175
BbrPI CACGTG 1 cut(s) 1197
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 1196
BbvI GCAGC 4 cut(s) 201, 358, 938, 1291
BccI CCATC 2 cut(s) 851, 1130
BceAI ACGGC 3 cut(s) 164, 416, 1084
BciT130I CCWGG 3 cut(s) 588, 1020, 1263
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 5 cut(s) 692, 810, 1093, 1229, 1417
BfaI CTAG 3 cut(s) 828, 912, 965
BfmI CTRYAG 1 cut(s) 1165
BglII AGATCT 1 cut(s) 1081
BisI GCNGC 7 cut(s) 215, 282, 372, 927, 930, 933, 1280
BlsI GCNGC 7 cut(s) 216, 283, 373, 928, 931, 934, 1281
Bme1390I CCNGG 3 cut(s) 588, 1020, 1263
BmeT110I CYCGRG 1 cut(s) 174
BmgT120I GGNCC 1 cut(s) 364
BmiI GGNNCC 3 cut(s) 366, 501, 801
BmrFI CCNGG 3 cut(s) 588, 1020, 1263
BmrI ACTGGG 1 cut(s) 1400
BmsI GCATC 3 cut(s) 226, 418, 1372
BmuI ACTGGG 1 cut(s) 1400
BoxI GACNNNNGTC 1 cut(s) 659
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 2 cut(s) 609, 1284
Bpu14I TTCGAA 2 cut(s) 757, 1354
BpuEI CTTGAG 2 cut(s) 314, 1210
BsaAI YACGTR 1 cut(s) 1197
BsaBI GATNNNNATC 1 cut(s) 498
BsaI GGTCTC 2 cut(s) 810, 1417
BsaJI CCNNGG 6 cut(s) 146, 687, 930, 1018, 1155, 1261
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 1019
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 1395
Bse3DI GCAATG 1 cut(s) 1428
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 3 cut(s) 588, 1020, 1263
BseDI CCNNGG 6 cut(s) 146, 687, 930, 1018, 1155, 1261
BseGI GGATG 2 cut(s) 241, 583
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 2 cut(s) 141, 1019
BseMI GCAATG 1 cut(s) 1428
BseMII CTCAG 2 cut(s) 444, 1361
BseNI ACTGG 1 cut(s) 1395
BseRI GAGGAG 1 cut(s) 1349
BseX3I CGGCCG 1 cut(s) 282
BseXI GCAGC 4 cut(s) 201, 358, 938, 1291
BseYI CCCAGC 2 cut(s) 367, 1454
BsgI GTGCAG 2 cut(s) 27, 1431
Bsh1236I CGCG 1 cut(s) 932
Bsh1285I CGRYCG 1 cut(s) 285
BshFI GGCC 6 cut(s) 131, 145, 284, 365, 1071, 1232
BsiEI CGRYCG 1 cut(s) 285
BsiHKAI GWGCWC 1 cut(s) 1196
BsiHKCI CYCGRG 1 cut(s) 174
BsiSI CCGG 1 cut(s) 195
BslI CCNNNNNNNGG 2 cut(s) 141, 1019
BsmAI GTCTC 5 cut(s) 692, 810, 1093, 1229, 1417
BsnI GGCC 6 cut(s) 131, 145, 284, 365, 1071, 1232
Bso31I GGTCTC 2 cut(s) 810, 1417
BsoBI CYCGRG 1 cut(s) 174
Bsp119I TTCGAA 2 cut(s) 757, 1354
Bsp1286I GDGCHC 2 cut(s) 175, 1196
Bsp143I GATC 7 cut(s) 103, 403, 499, 598, 670, 1009, 1081
BspACI CCGC 4 cut(s) 281, 318, 930, 932
BspANI GGCC 6 cut(s) 131, 145, 284, 365, 1071, 1232
BspCNI CTCAG 2 cut(s) 445, 1362
BspFNI CGCG 1 cut(s) 932
BspHI TCATGA 1 cut(s) 726
BspLI GGNNCC 3 cut(s) 366, 501, 801
BspPI GGATC 4 cut(s) 494, 507, 606, 1004
BspT104I TTCGAA 2 cut(s) 757, 1354
BspTNI GGTCTC 2 cut(s) 810, 1417
BsrDI GCAATG 1 cut(s) 1428
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 1 cut(s) 1395
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 6 cut(s) 146, 687, 930, 1018, 1155, 1261
BssMI GATC 7 cut(s) 103, 403, 499, 598, 670, 1009, 1081
BssT1I CCWWGG 3 cut(s) 146, 687, 1155
Bst2UI CCWGG 3 cut(s) 588, 1020, 1263
Bst4CI ACNGT 2 cut(s) 480, 1360
BstBAI YACGTR 1 cut(s) 1197
BstBI TTCGAA 2 cut(s) 757, 1354
BstC8I GCNNGC 2 cut(s) 129, 630
BstDEI CTNAG 4 cut(s) 453, 838, 1370, 1379
BstDSI CCRYGG 1 cut(s) 930
BstEII GGTNACC 1 cut(s) 1139
BstF5I GGATG 2 cut(s) 241, 583
BstFNI CGCG 1 cut(s) 932
BstKTI GATC 7 cut(s) 106, 406, 502, 601, 673, 1012, 1084
BstMAI GTCTC 5 cut(s) 692, 810, 1093, 1229, 1417
BstMBI GATC 7 cut(s) 103, 403, 499, 598, 670, 1009, 1081
BstMCI CGRYCG 1 cut(s) 285
BstMWI GCNNNNNNNGC 7 cut(s) 91, 371, 521, 932, 1322, 1375, 1439
BstNI CCWGG 3 cut(s) 588, 1020, 1263
BstPAI GACNNNNGTC 1 cut(s) 659
BstPI GGTNACC 1 cut(s) 1139
BstSCI CCNGG 3 cut(s) 586, 1018, 1261
BstSFI CTRYAG 1 cut(s) 1165
BstUI CGCG 1 cut(s) 932
BstV1I GCAGC 4 cut(s) 201, 358, 938, 1291
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 4 cut(s) 499, 598, 1009, 1081
BstXI CCANNNNNNTGG 3 cut(s) 347, 375, 694
BstYI RGATCY 4 cut(s) 499, 598, 1009, 1081
BstZI CGGCCG 1 cut(s) 282
BsuRI GGCC 6 cut(s) 131, 145, 284, 365, 1071, 1232
BtgI CCRYGG 1 cut(s) 930
BtsCI GGATG 2 cut(s) 241, 583
BtsIMutI CAGTG 1 cut(s) 1365
Cac8I GCNNGC 2 cut(s) 129, 630
CaiI CAGNNNCTG 1 cut(s) 127
CciI TCATGA 1 cut(s) 726
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 1 cut(s) 364
Cfr42I CCGCGG 1 cut(s) 933
Csp6I GTAC 1 cut(s) 1349
CviAII CATG 7 cut(s) 272, 313, 375, 407, 727, 1004, 1407
CviQI GTAC 1 cut(s) 1349
DdeI CTNAG 4 cut(s) 453, 838, 1370, 1379
DpnI GATC 7 cut(s) 105, 405, 501, 600, 672, 1011, 1083
DpnII GATC 7 cut(s) 103, 403, 499, 598, 670, 1009, 1081
EaeI YGGCCR 4 cut(s) 129, 282, 1069, 1230
EagI CGGCCG 1 cut(s) 282
EclXI CGGCCG 1 cut(s) 282
Eco130I CCWWGG 3 cut(s) 146, 687, 1155
Eco147I AGGCCT 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 175
Eco31I GGTCTC 2 cut(s) 810, 1417
Eco32I GATATC 3 cut(s) 187, 616, 1477
Eco52I CGGCCG 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 1007
Eco72I CACGTG 1 cut(s) 1197
Eco88I CYCGRG 1 cut(s) 174
Eco91I GGTNACC 1 cut(s) 1139
EcoO65I GGTNACC 1 cut(s) 1139
EcoRI GAATTC 1 cut(s) 716
EcoRII CCWGG 3 cut(s) 586, 1018, 1261
EcoRV GATATC 3 cut(s) 187, 616, 1477
EcoT14I CCWWGG 3 cut(s) 146, 687, 1155
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 3 cut(s) 146, 687, 1155
FaeI CATG 7 cut(s) 275, 316, 378, 410, 730, 1007, 1410
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FatI CATG 7 cut(s) 271, 312, 374, 406, 726, 1003, 1406
FauNDI CATATG 1 cut(s) 970
FbaI TGATCA 1 cut(s) 403
Fnu4HI GCNGC 7 cut(s) 215, 282, 372, 927, 930, 933, 1280
FokI GGATG 2 cut(s) 248, 570
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 7 cut(s) 215, 282, 372, 927, 930, 933, 1280
FspBI CTAG 3 cut(s) 828, 912, 965
GluI GCNGC 7 cut(s) 215, 282, 372, 927, 930, 933, 1280
GsaI CCCAGC 2 cut(s) 371, 1458
GsuI CTGGAG 2 cut(s) 609, 1284
HaeIII GGCC 6 cut(s) 131, 145, 284, 365, 1071, 1232
HapII CCGG 1 cut(s) 195
Hin1II CATG 7 cut(s) 275, 316, 378, 410, 730, 1007, 1410
HinfI GANTC 8 cut(s) 14, 489, 787, 834, 874, 982, 1295, 1395
HpaII CCGG 1 cut(s) 195
HphI GGTGA 6 cut(s) 391, 524, 563, 893, 929, 1151
Hpy166II GTNNAC 1 cut(s) 532
Hpy188I TCNGA 4 cut(s) 310, 987, 1048, 1291
Hpy188III TCNNGA 6 cut(s) 53, 596, 727, 871, 1331, 1519
Hpy8I GTNNAC 1 cut(s) 532
HpyAV CCTTC 2 cut(s) 226, 1207
HpyCH4III ACNGT 2 cut(s) 480, 1360
HpyCH4IV ACGT 1 cut(s) 1196
HpyF10VI GCNNNNNNNGC 7 cut(s) 91, 371, 521, 932, 1322, 1375, 1439
HpyF3I CTNAG 4 cut(s) 453, 838, 1370, 1379
HpySE526I ACGT 1 cut(s) 1196
Hsp92II CATG 7 cut(s) 275, 316, 378, 410, 730, 1007, 1410
Ksp22I TGATCA 1 cut(s) 403
KspI CCGCGG 1 cut(s) 933
Kzo9I GATC 7 cut(s) 103, 403, 499, 598, 670, 1009, 1081
LmnI GCTCC 3 cut(s) 161, 415, 1171
Lsp1109I GCAGC 4 cut(s) 201, 358, 938, 1291
LweI GCATC 3 cut(s) 226, 418, 1372
MaeI CTAG 3 cut(s) 828, 912, 965
MaeII ACGT 1 cut(s) 1196
MaeIII GTNAC 7 cut(s) 391, 620, 682, 823, 1139, 1267, 1512
MalI GATC 7 cut(s) 105, 405, 501, 600, 672, 1011, 1083
MboI GATC 7 cut(s) 103, 403, 499, 598, 670, 1009, 1081
MboII GAAGA 7 cut(s) 23, 52, 316, 991, 1000, 1290, 1448
MflI RGATCY 4 cut(s) 499, 598, 1009, 1081
MhlI GDGCHC 2 cut(s) 175, 1196
MlsI TGGCCA 1 cut(s) 131
MluCI AATT 8 cut(s) 61, 137, 342, 665, 716, 753, 888, 1253
MluNI TGGCCA 1 cut(s) 131
MlyI GAGTC 3 cut(s) 8, 781, 843
MmeI TCCRAC 1 cut(s) 1366
Mox20I TGGCCA 1 cut(s) 131
MroXI GAANNNNTTC 2 cut(s) 753, 995
MscI TGGCCA 1 cut(s) 131
MseI TTAA 3 cut(s) 1305, 1463, 1508
Msp20I TGGCCA 1 cut(s) 131
MspA1I CMGCKG 3 cut(s) 214, 371, 932
MspI CCGG 1 cut(s) 195
MspR9I CCNGG 3 cut(s) 588, 1020, 1263
MvaI CCWGG 3 cut(s) 588, 1020, 1263
MvnI CGCG 1 cut(s) 932
MwoI GCNNNNNNNGC 7 cut(s) 91, 371, 521, 932, 1322, 1375, 1439
NdeI CATATG 1 cut(s) 970
NdeII GATC 7 cut(s) 103, 403, 499, 598, 670, 1009, 1081
NlaIII CATG 7 cut(s) 275, 316, 378, 410, 730, 1007, 1410
NlaIV GGNNCC 3 cut(s) 366, 501, 801
NmeAIII GCCGAG 1 cut(s) 1258
NmuCI GTSAC 3 cut(s) 823, 1139, 1267
NspV TTCGAA 2 cut(s) 757, 1354
PaeR7I CTCGAG 1 cut(s) 174
PagI TCATGA 1 cut(s) 726
PceI AGGCCT 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 811
PdmI GAANNNNTTC 2 cut(s) 753, 995
PfeI GAWTC 5 cut(s) 489, 874, 982, 1295, 1395
PflFI GACNNNGTC 1 cut(s) 821
PfoI TCCNGGA 1 cut(s) 586
PkrI GCNGC 7 cut(s) 216, 283, 373, 928, 931, 934, 1281
PleI GAGTC 3 cut(s) 8, 781, 842
PmaCI CACGTG 1 cut(s) 1197
PmlI CACGTG 1 cut(s) 1197
PpsI GAGTC 3 cut(s) 8, 781, 842
Ppu21I YACGTR 1 cut(s) 1197
PshAI GACNNNNGTC 1 cut(s) 659
Psp6I CCWGG 3 cut(s) 586, 1018, 1261
PspCI CACGTG 1 cut(s) 1197
PspEI GGTNACC 1 cut(s) 1139
PspFI CCCAGC 2 cut(s) 367, 1454
PspGI CCWGG 3 cut(s) 586, 1018, 1261
PspN4I GGNNCC 3 cut(s) 366, 501, 801
PspPI GGNCC 1 cut(s) 364
PspXI VCTCGAGB 1 cut(s) 174
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 4 cut(s) 499, 598, 1009, 1081
PsyI GACNNNGTC 1 cut(s) 821
PvuII CAGCTG 2 cut(s) 214, 371
RsaI GTAC 1 cut(s) 1350
RsaNI GTAC 1 cut(s) 1349
SacII CCGCGG 1 cut(s) 933
SaqAI TTAA 3 cut(s) 1305, 1463, 1508
SatI GCNGC 7 cut(s) 215, 282, 372, 927, 930, 933, 1280
Sau3AI GATC 7 cut(s) 103, 403, 499, 598, 670, 1009, 1081
Sau96I GGNCC 1 cut(s) 364
SchI GAGTC 3 cut(s) 8, 781, 843
ScrFI CCNGG 3 cut(s) 588, 1020, 1263
SduI GDGCHC 2 cut(s) 175, 1196
SfaNI GCATC 3 cut(s) 226, 418, 1372
SfcI CTRYAG 1 cut(s) 1165
Sfr274I CTCGAG 1 cut(s) 174
Sfr303I CCGCGG 1 cut(s) 933
SfuI TTCGAA 2 cut(s) 757, 1354
SgrBI CCGCGG 1 cut(s) 933
SlaI CTCGAG 1 cut(s) 174
SmlI CTYRAG 3 cut(s) 174, 329, 1189
SmoI CTYRAG 3 cut(s) 174, 329, 1189
Sse9I AATT 8 cut(s) 61, 137, 342, 665, 716, 753, 888, 1253
SseBI AGGCCT 1 cut(s) 145
SsiI CCGC 4 cut(s) 281, 318, 930, 932
SspMI CTAG 3 cut(s) 828, 912, 965
StuI AGGCCT 1 cut(s) 145
StyD4I CCNGG 3 cut(s) 586, 1018, 1261
StyI CCWWGG 3 cut(s) 146, 687, 1155
TaaI ACNGT 2 cut(s) 480, 1360
TaiI ACGT 1 cut(s) 1199
TaqI TCGA 8 cut(s) 175, 463, 492, 518, 720, 757, 805, 1354
TasI AATT 8 cut(s) 61, 137, 342, 665, 716, 753, 888, 1253
TatI WGTACW 1 cut(s) 1348
TauI GCSGC 3 cut(s) 284, 932, 935
TfiI GAWTC 5 cut(s) 489, 874, 982, 1295, 1395
Tru1I TTAA 3 cut(s) 1305, 1463, 1508
Tru9I TTAA 3 cut(s) 1305, 1463, 1508
TscAI CASTG 1 cut(s) 1365
TseFI GTSAC 3 cut(s) 823, 1139, 1267
TseI GCWGC 4 cut(s) 214, 371, 926, 1279
Tsp45I GTSAC 3 cut(s) 823, 1139, 1267
TspDTI ATGAA 5 cut(s) 288, 715, 729, 900, 1221
TspGWI ACGGA 2 cut(s) 608, 670
TspRI CASTG 1 cut(s) 1365
Tth111I GACNNNGTC 1 cut(s) 821
XapI RAATTY 3 cut(s) 665, 716, 753
XhoI CTCGAG 1 cut(s) 174
XmnI GAANNNNTTC 2 cut(s) 753, 995
XspI CTAG 3 cut(s) 828, 912, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.