FvH4_5g07580

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005306
Physical Location & Seq
Forward (+)
4421094 .. 4425483
4390 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07580.t3

Sequence Viewer

Length: 1452 bp
ATGCTTCCTCTGCCTCCTATTGATCTCAACGATGTTCAGCACAAGCTGGCCACCCAGTTCAGGCCGTGGCAACGCTCCTTTCAGTTCTGGGCTCGAGCCGTCGACATCTACACTGGCTACAAGGTGTTTCAGCTGCGTGTGAGTTTCGTGAAGGATGTGAAGAGACAAGAGGAAATGTGGGAGAGGCAACATGAAGTTGCGGCTGAGAAGATATATGCTATGTGTTCGGACATGGGCGGTTTCTTTCTCAAGGTTGCCCAAATTGTTGGGAAGCCAGACTTGGCCCCTGTTGCCTGGGTGAAAAGGCTTGTTACATTGTGTGATCATGCTCCTGCTACTCCATTTGATGCCGTTCAACTTATGCTGGAAACTGAGTTGGGTCGAAGTATTGGTGAAGTGTTTGAGAGATTCGATGTGGAACCTATTGGCTCTGCTTCAATTGCACAGGTTCACCGAGCAAGATTGAGAGGTGATAAGAATGATATTGTTGTCAAGGTGCAACATCCTGGAGTTCAGGATCTAATGATGACAGATATCCGTAACTTGCAAGCCTTTGCTTTATACATTCAGAAGACAGATGTCAAGTTTGATCTATACTCTGTAACCAAGGAAATGGAGACTCAGATCGGATATGAATTTGACTTCACAAGAGAGGCTAATGCCATGGAAAAGATTCAAAAATTTTTGTATGAAAATAACAAAAGGACTCCTGTCGTGGTTCCTCGACTAATACGAAACCTAGTCACTAGGAGAGTTTTAGTTATGGAATATATTGATGGGATACCAATCCTCAATCTTGGAGATGAAATTGCGAAAAGGGGTATAAATCCTGTTGGTAAGGTTGCAGCCGCAGCAAAGCAGAAAATCCTCCAAAGTTTGACACTAGCATATGGACAAATGATTTTGAAGAATGGTTTCTTCCATGCAGATCCCCACCCAGGAAATATCCTAATCTGTAAAGGTTCAGAGGTTGCCTTGCTCGATTATGGCCAAGTGAAAGATCTCCCAGATCAGTTGAGACTTGGTTATGCTAATCTTATTCTTGCGATAGCTGATGGTGACCCTGTAAGAGCCTTAGAGAGCTATGGGGAGCTTGGCATAGAAACCTTAAGCACATGTGAAGATGAACAAAAGGAAATGTTGAAGTTGGCAGAGACAATGTTTGACACGAAATTACCCCCTGGAGTGACGATGTTGCAGCCTTTTTCCGAGGAATCTTCTATTAAAAAGATTGCAGTTCAGGCTTTTCCAGAGGAGTTGTTTTCTGTACTTCGAACAGTGCACCTGCTTAGAGGGCTTAGTGTTGGACTGGGAATCAACTACTCATGTGCAGAACAATGGAGACCAATTGCAGAAGAAGCTTTGATTCGTGCTGGGAGATTAAAAGGTAAAGATCTCAAGGCTAGAAATCGTAGAGGTGGTTTATTAAGGAGGTTACTCTGGAGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

484

Amino Acids

54.77

Weight (kDa)

8.59

Isoelectric Point (pI)

37.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 106 - 359 1.4e-62 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1293
Acc36I ACCTGC 1 cut(s) 1293
AccI GTMKAC 1 cut(s) 102
AciI CCGC 3 cut(s) 200, 237, 849
AclWI GGATC 2 cut(s) 525, 923
AcoI YGGCCR 2 cut(s) 48, 988
AcsI RAATTY 2 cut(s) 635, 680
AfaI GTAC 1 cut(s) 1269
AfiI CCNNNNNNNGG 2 cut(s) 60, 938
AflII CTTAAG 1 cut(s) 1108
AflIII ACRYGT 1 cut(s) 1115
AgsI TTSAA 5 cut(s) 356, 438, 677, 907, 1144
AjnI CCWGG 4 cut(s) 293, 505, 937, 1180
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 6 cut(s) 46, 133, 1052, 1083, 1093, 1361
AluI AGCT 6 cut(s) 46, 133, 1052, 1083, 1093, 1361
Alw21I GWGCWC 1 cut(s) 1284
Alw26I GTCTC 5 cut(s) 157, 611, 1012, 1148, 1336
Alw44I GTGCAC 1 cut(s) 1280
AlwI GGATC 2 cut(s) 525, 923
Ama87I CYCGRG 1 cut(s) 93
AoxI GGCC 4 cut(s) 48, 62, 282, 988
ApaLI GTGCAC 1 cut(s) 1280
ApeKI GCWGC 4 cut(s) 133, 845, 851, 1198
ApoI RAATTY 2 cut(s) 635, 680
Asp700I GAANNNNTTC 2 cut(s) 672, 914
AspS9I GGNCC 1 cut(s) 283
AsuHPI GGTGA 5 cut(s) 310, 404, 443, 482, 1070
AsuII TTCGAA 1 cut(s) 1273
AvaI CYCGRG 1 cut(s) 93
BaeGI GKGCMC 1 cut(s) 1284
BalI TGGCCA 2 cut(s) 50, 990
BanII GRGCYC 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 578
Bbv12I GWGCWC 1 cut(s) 1284
BbvI GCAGC 4 cut(s) 120, 857, 863, 1210
BccI CCATC 2 cut(s) 770, 1049
BceAI ACGGC 3 cut(s) 49, 83, 335
BciT130I CCWGG 4 cut(s) 295, 507, 939, 1182
BciVI GTATCC 1 cut(s) 774
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 5 cut(s) 157, 611, 1012, 1148, 1336
BfaI CTAG 4 cut(s) 740, 747, 884, 1404
BfrI CTTAAG 1 cut(s) 1108
BfuAI ACCTGC 1 cut(s) 1293
BfuI GTATCC 1 cut(s) 774
BglII AGATCT 2 cut(s) 1000, 1393
BisI GCNGC 6 cut(s) 134, 201, 846, 849, 852, 1199
BlsI GCNGC 6 cut(s) 135, 202, 847, 850, 853, 1200
Bme1390I CCNGG 4 cut(s) 295, 507, 939, 1182
BmeT110I CYCGRG 1 cut(s) 93
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 3 cut(s) 285, 420, 720
BmrFI CCNGG 4 cut(s) 295, 507, 939, 1182
BmrI ACTGGG 2 cut(s) 49, 1319
BmsI GCATC 1 cut(s) 337
BmuI ACTGGG 2 cut(s) 49, 1319
BoxI GACNNNNGTC 2 cut(s) 578, 710
BpiI GAAGAC 1 cut(s) 578
BpmI CTGGAG 2 cut(s) 528, 1203
Bpu14I TTCGAA 1 cut(s) 1273
BpuEI CTTGAG 2 cut(s) 233, 1382
BsaBI GATNNNNATC 1 cut(s) 785
BsaI GGTCTC 1 cut(s) 1336
BsaJI CCNNGG 7 cut(s) 65, 294, 606, 663, 937, 1180, 1209
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 938
Bse1I ACTGG 3 cut(s) 55, 118, 1314
Bse8I GATNNNNATC 1 cut(s) 785
BseBI CCWGG 4 cut(s) 295, 507, 939, 1182
BseDI CCNNGG 7 cut(s) 65, 294, 606, 663, 937, 1180, 1209
BseGI GGATG 2 cut(s) 160, 502
BseJI GATNNNNATC 1 cut(s) 785
BseLI CCNNNNNNNGG 2 cut(s) 60, 938
BseMII CTCAG 3 cut(s) 195, 363, 635
BseNI ACTGG 3 cut(s) 55, 118, 1314
BseRI GAGGAG 1 cut(s) 1268
BseSI GKGCMC 1 cut(s) 1284
BseXI GCAGC 4 cut(s) 120, 857, 863, 1210
BseYI CCCAGC 1 cut(s) 1373
BsgI GTGCAG 1 cut(s) 1350
BshFI GGCC 4 cut(s) 50, 64, 284, 990
BsiHKAI GWGCWC 1 cut(s) 1284
BsiHKCI CYCGRG 1 cut(s) 93
BslI CCNNNNNNNGG 2 cut(s) 60, 938
BsmAI GTCTC 5 cut(s) 157, 611, 1012, 1148, 1336
BsnI GGCC 4 cut(s) 50, 64, 284, 990
Bso31I GGTCTC 1 cut(s) 1336
BsoBI CYCGRG 1 cut(s) 93
Bsp119I TTCGAA 1 cut(s) 1273
Bsp1286I GDGCHC 2 cut(s) 94, 1284
Bsp143I GATC 9 cut(s) 22, 322, 517, 589, 624, 928, 1000, 1009, 1393
Bsp19I CCATGG 1 cut(s) 663
BspACI CCGC 3 cut(s) 200, 237, 849
BspANI GGCC 4 cut(s) 50, 64, 284, 990
BspCNI CTCAG 3 cut(s) 196, 364, 634
BspLI GGNNCC 3 cut(s) 285, 420, 720
BspMI ACCTGC 1 cut(s) 1293
BspPI GGATC 2 cut(s) 525, 923
BspT104I TTCGAA 1 cut(s) 1273
BspTI CTTAAG 1 cut(s) 1108
BspTNI GGTCTC 1 cut(s) 1336
BsrI ACTGG 3 cut(s) 55, 118, 1314
BssECI CCNNGG 7 cut(s) 65, 294, 606, 663, 937, 1180, 1209
BssMI GATC 9 cut(s) 22, 322, 517, 589, 624, 928, 1000, 1009, 1393
BssT1I CCWWGG 2 cut(s) 606, 663
Bst2UI CCWGG 4 cut(s) 295, 507, 939, 1182
Bst4CI ACNGT 1 cut(s) 1279
Bst6I CTCTTC 1 cut(s) 155
BstAFI CTTAAG 1 cut(s) 1108
BstBI TTCGAA 1 cut(s) 1273
BstC8I GCNNGC 2 cut(s) 48, 549
BstDEI CTNAG 6 cut(s) 204, 372, 621, 1075, 1289, 1298
BstDSI CCRYGG 2 cut(s) 65, 663
BstEII GGTNACC 1 cut(s) 1058
BstF5I GGATG 2 cut(s) 160, 502
BstKTI GATC 9 cut(s) 25, 325, 520, 592, 627, 931, 1003, 1012, 1396
BstMAI GTCTC 5 cut(s) 157, 611, 1012, 1148, 1336
BstMBI GATC 9 cut(s) 22, 322, 517, 589, 624, 928, 1000, 1009, 1393
BstMWI GCNNNNNNNGC 7 cut(s) 10, 290, 440, 851, 1241, 1294, 1358
BstNI CCWGG 4 cut(s) 295, 507, 939, 1182
BstNSI RCATGY 1 cut(s) 1119
BstPAI GACNNNNGTC 2 cut(s) 578, 710
BstPI GGTNACC 1 cut(s) 1058
BstSCI CCNGG 4 cut(s) 293, 505, 937, 1180
BstSLI GKGCMC 1 cut(s) 1284
BstV1I GCAGC 4 cut(s) 120, 857, 863, 1210
BstV2I GAAGAC 1 cut(s) 578
BstX2I RGATCY 4 cut(s) 517, 928, 1000, 1393
BstXI CCANNNNNNTGG 2 cut(s) 266, 613
BstYI RGATCY 4 cut(s) 517, 928, 1000, 1393
BsuI GTATCC 1 cut(s) 774
BsuRI GGCC 4 cut(s) 50, 64, 284, 990
BtgI CCRYGG 2 cut(s) 65, 663
BtsCI GGATG 2 cut(s) 160, 502
BtsIMutI CAGTG 2 cut(s) 111, 1284
BveI ACCTGC 1 cut(s) 1293
Cac8I GCNNGC 2 cut(s) 48, 549
Cfr13I GGNCC 1 cut(s) 283
Csp6I GTAC 1 cut(s) 1268
CviAII CATG 7 cut(s) 191, 232, 326, 664, 923, 1116, 1326
CviQI GTAC 1 cut(s) 1268
DdeI CTNAG 6 cut(s) 204, 372, 621, 1075, 1289, 1298
DpnI GATC 9 cut(s) 24, 324, 519, 591, 626, 930, 1002, 1011, 1395
DpnII GATC 9 cut(s) 22, 322, 517, 589, 624, 928, 1000, 1009, 1393
EaeI YGGCCR 2 cut(s) 48, 988
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
Eco130I CCWWGG 2 cut(s) 606, 663
Eco24I GRGCYC 1 cut(s) 94
Eco31I GGTCTC 1 cut(s) 1336
Eco32I GATATC 1 cut(s) 535
Eco88I CYCGRG 1 cut(s) 93
Eco91I GGTNACC 1 cut(s) 1058
EcoO65I GGTNACC 1 cut(s) 1058
EcoRII CCWGG 4 cut(s) 293, 505, 937, 1180
EcoRV GATATC 1 cut(s) 535
EcoT14I CCWWGG 2 cut(s) 606, 663
EcoT38I GRGCYC 1 cut(s) 94
ErhI CCWWGG 2 cut(s) 606, 663
FaeI CATG 7 cut(s) 194, 235, 329, 667, 926, 1119, 1329
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FatI CATG 7 cut(s) 190, 231, 325, 663, 922, 1115, 1325
FauNDI CATATG 1 cut(s) 889
FbaI TGATCA 1 cut(s) 322
FblI GTMKAC 1 cut(s) 102
Fnu4HI GCNGC 6 cut(s) 134, 201, 846, 849, 852, 1199
FokI GGATG 2 cut(s) 167, 489
FriOI GRGCYC 1 cut(s) 94
Fsp4HI GCNGC 6 cut(s) 134, 201, 846, 849, 852, 1199
FspBI CTAG 4 cut(s) 740, 747, 884, 1404
GluI GCNGC 6 cut(s) 134, 201, 846, 849, 852, 1199
GsaI CCCAGC 1 cut(s) 1377
GsuI CTGGAG 2 cut(s) 528, 1203
HaeIII GGCC 4 cut(s) 50, 64, 284, 990
Hin1II CATG 7 cut(s) 194, 235, 329, 667, 926, 1119, 1329
HincII GTYRAC 1 cut(s) 103
HindII GTYRAC 1 cut(s) 103
HindIII AAGCTT 1 cut(s) 1359
HinfI GANTC 7 cut(s) 408, 619, 673, 706, 1214, 1314, 1366
HphI GGTGA 5 cut(s) 310, 404, 443, 482, 1070
Hpy166II GTNNAC 3 cut(s) 103, 451, 1282
Hpy188I TCNGA 6 cut(s) 229, 570, 624, 629, 967, 1210
Hpy188III TCNNGA 4 cut(s) 148, 515, 1250, 1441
Hpy8I GTNNAC 3 cut(s) 103, 451, 1282
Hpy99I CGWCG 1 cut(s) 104
HpyAV CCTTC 1 cut(s) 145
HpyCH4III ACNGT 1 cut(s) 1279
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 290, 440, 851, 1241, 1294, 1358
HpyF3I CTNAG 6 cut(s) 204, 372, 621, 1075, 1289, 1298
Hsp92II CATG 7 cut(s) 194, 235, 329, 667, 926, 1119, 1329
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 9 cut(s) 22, 322, 517, 589, 624, 928, 1000, 1009, 1393
LmnI GCTCC 3 cut(s) 80, 334, 1090
Lsp1109I GCAGC 4 cut(s) 120, 857, 863, 1210
LweI GCATC 1 cut(s) 337
MaeI CTAG 4 cut(s) 740, 747, 884, 1404
MaeIII GTNAC 7 cut(s) 310, 539, 601, 742, 1058, 1186, 1434
MalI GATC 9 cut(s) 24, 324, 519, 591, 626, 930, 1002, 1011, 1395
MboI GATC 9 cut(s) 22, 322, 517, 589, 624, 928, 1000, 1009, 1393
MboII GAAGA 8 cut(s) 172, 220, 583, 910, 919, 1133, 1209, 1367
MfeI CAATTG 2 cut(s) 438, 1347
MflI RGATCY 4 cut(s) 517, 928, 1000, 1393
MhlI GDGCHC 2 cut(s) 94, 1284
MlsI TGGCCA 2 cut(s) 50, 990
MluCI AATT 7 cut(s) 261, 438, 635, 680, 807, 1172, 1347
MluNI TGGCCA 2 cut(s) 50, 990
MlyI GAGTC 2 cut(s) 613, 700
MmeI TCCRAC 1 cut(s) 1285
Mox20I TGGCCA 2 cut(s) 50, 990
MroXI GAANNNNTTC 2 cut(s) 672, 914
MscI TGGCCA 2 cut(s) 50, 990
MseI TTAA 4 cut(s) 1109, 1224, 1382, 1427
Msp20I TGGCCA 2 cut(s) 50, 990
MspA1I CMGCKG 1 cut(s) 133
MspCI CTTAAG 1 cut(s) 1108
MspR9I CCNGG 4 cut(s) 295, 507, 939, 1182
MunI CAATTG 2 cut(s) 438, 1347
MvaI CCWGG 4 cut(s) 295, 507, 939, 1182
MwoI GCNNNNNNNGC 7 cut(s) 10, 290, 440, 851, 1241, 1294, 1358
NcoI CCATGG 1 cut(s) 663
NdeI CATATG 1 cut(s) 889
NdeII GATC 9 cut(s) 22, 322, 517, 589, 624, 928, 1000, 1009, 1393
NlaIII CATG 7 cut(s) 194, 235, 329, 667, 926, 1119, 1329
NlaIV GGNNCC 3 cut(s) 285, 420, 720
NmuCI GTSAC 3 cut(s) 742, 1058, 1186
NspI RCATGY 1 cut(s) 1119
NspV TTCGAA 1 cut(s) 1273
PaeR7I CTCGAG 1 cut(s) 93
PaqCI CACCTGC 1 cut(s) 1293
PciI ACATGT 1 cut(s) 1115
PcsI WCGNNNNNNNCGW 1 cut(s) 730
PdmI GAANNNNTTC 2 cut(s) 672, 914
PfeI GAWTC 5 cut(s) 408, 673, 1214, 1314, 1366
PfoI TCCNGGA 1 cut(s) 505
PkrI GCNGC 6 cut(s) 135, 202, 847, 850, 853, 1200
PleI GAGTC 2 cut(s) 613, 700
PpsI GAGTC 2 cut(s) 613, 700
PscI ACATGT 1 cut(s) 1115
PshAI GACNNNNGTC 2 cut(s) 578, 710
Psp6I CCWGG 4 cut(s) 293, 505, 937, 1180
PspEI GGTNACC 1 cut(s) 1058
PspFI CCCAGC 1 cut(s) 1373
PspGI CCWGG 4 cut(s) 293, 505, 937, 1180
PspN4I GGNNCC 3 cut(s) 285, 420, 720
PspPI GGNCC 1 cut(s) 283
PspXI VCTCGAGB 1 cut(s) 93
PsuI RGATCY 4 cut(s) 517, 928, 1000, 1393
PvuII CAGCTG 1 cut(s) 133
RsaI GTAC 1 cut(s) 1269
RsaNI GTAC 1 cut(s) 1268
SalI GTCGAC 1 cut(s) 101
SaqAI TTAA 4 cut(s) 1109, 1224, 1382, 1427
SatI GCNGC 6 cut(s) 134, 201, 846, 849, 852, 1199
Sau3AI GATC 9 cut(s) 22, 322, 517, 589, 624, 928, 1000, 1009, 1393
Sau96I GGNCC 1 cut(s) 283
SchI GAGTC 2 cut(s) 613, 700
ScrFI CCNGG 4 cut(s) 295, 507, 939, 1182
SduI GDGCHC 2 cut(s) 94, 1284
SfaNI GCATC 1 cut(s) 337
Sfr274I CTCGAG 1 cut(s) 93
SfuI TTCGAA 1 cut(s) 1273
SlaI CTCGAG 1 cut(s) 93
SmlI CTYRAG 4 cut(s) 93, 248, 1108, 1397
SmoI CTYRAG 4 cut(s) 93, 248, 1108, 1397
Sse9I AATT 7 cut(s) 261, 438, 635, 680, 807, 1172, 1347
SsiI CCGC 3 cut(s) 200, 237, 849
SspMI CTAG 4 cut(s) 740, 747, 884, 1404
StyD4I CCNGG 4 cut(s) 293, 505, 937, 1180
StyI CCWWGG 2 cut(s) 606, 663
TaaI ACNGT 1 cut(s) 1279
TaqI TCGA 7 cut(s) 94, 102, 382, 411, 724, 981, 1273
TasI AATT 7 cut(s) 261, 438, 635, 680, 807, 1172, 1347
TatI WGTACW 1 cut(s) 1267
TauI GCSGC 2 cut(s) 203, 851
TfiI GAWTC 5 cut(s) 408, 673, 1214, 1314, 1366
Tru1I TTAA 4 cut(s) 1109, 1224, 1382, 1427
Tru9I TTAA 4 cut(s) 1109, 1224, 1382, 1427
TscAI CASTG 2 cut(s) 118, 1284
TseFI GTSAC 3 cut(s) 742, 1058, 1186
TseI GCWGC 4 cut(s) 133, 845, 851, 1198
Tsp45I GTSAC 3 cut(s) 742, 1058, 1186
TspDTI ATGAA 5 cut(s) 207, 648, 705, 819, 1140
TspGWI ACGGA 1 cut(s) 527
TspRI CASTG 2 cut(s) 118, 1284
Vha464I CTTAAG 1 cut(s) 1108
VneI GTGCAC 1 cut(s) 1280
XapI RAATTY 2 cut(s) 635, 680
XceI RCATGY 1 cut(s) 1119
XhoI CTCGAG 1 cut(s) 93
XmiI GTMKAC 1 cut(s) 102
XmnI GAANNNNTTC 2 cut(s) 672, 914
XspI CTAG 4 cut(s) 740, 747, 884, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.