MD17G1259300.v1.1

AarF domain-containing protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005306
Physical Location & Seq
Reverse (-)
32008277 .. 32013097
4821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1259300.v1.1.491

Sequence Viewer

Length: 1449 bp
ATGCTTCCTCTGCCTCCCGTTGATCTCAAAGATGTTCAGCAGAATCTAGCAACGCATTTTCGGCCATGGCAGCGCTCCTTTCAGTTCTGGGTTCGAGCTGCTGATATCTACACGGGCTACAAGGTGTTTCAGCTGAGAGTGAGTTTGGTGAAGGATGCGAAGAAACAGGAGGAAATGTGGGAGAGGCAGCATGAATTTGCAGCTGAGAAGATATATGCTATGTGCTCGGACCTTGGCGGTTTCTTCCTTAAGGTTGCCCAGATCATCGGGAAGCCAGACTTGGCCCCGGCTGCATGGGTCAAAAGGCTTGTTCCATTGTGTGATCACGCTCCTGCAACGCCATTTGATGCTGTTCAACTTATGTTGGAGATGGAGTTGGGCCGAAATGTTGGTGAAGTGTTTGAAAGATTCGATGTGGATCCTCTTGGCTCTGCCTCAATTGCCCAGGTTCACCGAGCAAGATTGAGAGGTGATAAGAGTGATGTTGTTGTCAAGGTGCAACATCCTGGAGTTCAGGATCTAATGATGACCGATATTCGCAACTTGCAAGCCTTCGCCTTTTACATACAGAAGACAGACGTCAAATTTGATCTATACTCTGTAACAAAGGAAATGGAGAAACAGATTGGATACGAATTTGATTTCATGAGGGAGGCTGATGCTATGGAAAAAATTAGACATTTTTTGTATGAGAATAACAATAAGTCTCCAGTTTTGGTGCCACGACTCATACGAGATATAGTCACTAGGAGAGTTATAGTTATGGAATATATTGATGGAATCCCCATCCTGAATCTGGGTGATGAAATTGCAAAACGAGGCATAAATCCTGGTGGTAAGATTGCAGTAGCGGCAAAGCAGAAAATCCTCCAAAGTTTGACGCTGGCATATGGGCAAATGATTCTGAAGAATGGTTTCTTTCATGCCGATCCTCATCCAGGAAATATACTAATCTGTAAAGGCTCAGAGGTTGCCTTGCTGGATTATGGCCAAGTGAAAGATCTACCGGATGAGTTGAGACTTGGTTATGCTAATCTTGTCATTGCCATTGCTGATGGTGACCCTATTAGAGCATCAGAGAGCTACAGGGAACTTGGCATAGAAACCTTAAGCAAATGTGAAAATGAACAGCATGAATTGTTAAAGTTGGCACAGACAATGTTTGACACAAAATTACCCCCCGGAGTGACAATGCTGCAGCCTTTTTCCGAGGAATCTTCAATTAAAAAAATTGGAGTTCAGGCTTTTCCAGAGGAGTTGTTTTCTGTACTTCGGACAGTGCACCTGCTGAGAGGACTTAGTGTTGGTTTAGGAATCAACTACTCTTGTGCGGAACAATGGAAACCCATTGCAGAAGAAGCTTTGTTCCGTGCTGGGAGATTAAAAGGTAGGCCGATCAAAGCCAGAGGTCGTCGGCGTGGTTTATCGAGGTTATTTTCGAGAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ABC1

Protein Analysis

483

Amino Acids

54.64

Weight (kDa)

8.58

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 106 - 357 5.6e-61 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1293
AatII GACGTC 1 cut(s) 582
Acc36I ACCTGC 1 cut(s) 1293
AccB1I GGYRCC 1 cut(s) 718
AciI CCGC 3 cut(s) 237, 851, 1331
AclWI GGATC 4 cut(s) 413, 426, 525, 923
AcoI YGGCCR 2 cut(s) 62, 988
AcsI RAATTY 3 cut(s) 194, 584, 635
AcuI CTGAAG 1 cut(s) 926
AcyI GRCGYC 1 cut(s) 579
AfaI GTAC 1 cut(s) 1269
AfeI AGCGCT 1 cut(s) 74
AfiI CCNNNNNNNGG 2 cut(s) 796, 938
AflII CTTAAG 2 cut(s) 248, 1108
AgsI TTSAA 3 cut(s) 356, 404, 1221
AjnI CCWGG 4 cut(s) 444, 505, 829, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AluBI AGCT 5 cut(s) 98, 133, 203, 1083, 1361
AluI AGCT 5 cut(s) 98, 133, 203, 1083, 1361
Alw21I GWGCWC 2 cut(s) 227, 1284
Alw26I GTCTC 3 cut(s) 711, 1012, 1437
Alw44I GTGCAC 1 cut(s) 1280
AlwI GGATC 4 cut(s) 413, 426, 525, 923
Aor51HI AGCGCT 1 cut(s) 74
AoxI GGCC 5 cut(s) 62, 282, 379, 988, 1391
ApaLI GTGCAC 1 cut(s) 1280
ApeKI GCWGC 7 cut(s) 70, 98, 187, 200, 290, 1195, 1198
ApoI RAATTY 3 cut(s) 194, 584, 635
ArsI GACNNNNNNTTYG 2 cut(s) 569, 601
Asp700I GAANNNNTTC 1 cut(s) 914
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 3 cut(s) 229, 283, 379
AsuC2I CCSGG 2 cut(s) 287, 1182
AsuHPI GGTGA 6 cut(s) 160, 404, 443, 482, 812, 1070
AvaII GGWCC 1 cut(s) 229
BaeGI GKGCMC 1 cut(s) 1284
BalI TGGCCA 1 cut(s) 990
BamHI GGATCC 1 cut(s) 418
BanI GGYRCC 1 cut(s) 718
BbsI GAAGAC 1 cut(s) 578
Bbv12I GWGCWC 2 cut(s) 227, 1284
BbvI GCAGC 7 cut(s) 82, 85, 199, 212, 277, 1182, 1210
BccI CCATC 4 cut(s) 364, 770, 794, 1049
BciT130I CCWGG 4 cut(s) 446, 507, 831, 939
BciVI GTATCC 1 cut(s) 623
BclI TGATCA 1 cut(s) 322
BcnI CCSGG 2 cut(s) 287, 1182
BcoDI GTCTC 3 cut(s) 711, 1012, 1437
BfaI CTAG 2 cut(s) 47, 747
BfmI CTRYAG 2 cut(s) 1084, 1196
BfoI RGCGCY 1 cut(s) 76
BfrI CTTAAG 2 cut(s) 248, 1108
BfuAI ACCTGC 1 cut(s) 1293
BfuI GTATCC 1 cut(s) 623
BglII AGATCT 1 cut(s) 1000
BisI GCNGC 8 cut(s) 71, 99, 188, 201, 291, 852, 1196, 1199
BlsI GCNGC 8 cut(s) 72, 100, 189, 202, 292, 853, 1197, 1200
Bme1390I CCNGG 6 cut(s) 287, 446, 507, 831, 939, 1182
Bme18I GGWCC 1 cut(s) 229
BmgT120I GGNCC 3 cut(s) 229, 283, 379
BmiI GGNNCC 3 cut(s) 285, 420, 720
BmrFI CCNGG 6 cut(s) 287, 446, 507, 831, 939, 1182
BmsI GCATC 4 cut(s) 145, 337, 649, 1082
BoxI GACNNNNGTC 1 cut(s) 578
BpiI GAAGAC 1 cut(s) 578
BpmI CTGGAG 2 cut(s) 528, 693
BpuMI CCSGG 2 cut(s) 287, 1182
BsaBI GATNNNNATC 2 cut(s) 417, 933
BsaHI GRCGYC 1 cut(s) 579
BsaJI CCNNGG 6 cut(s) 65, 232, 285, 444, 1180, 1209
BsaWI WCCGGW 1 cut(s) 1006
BsaXI ACNNNNNCTCC 4 cut(s) 161, 191, 359, 389
Bsc4I CCNNNNNNNGG 2 cut(s) 796, 938
Bse1I ACTGG 1 cut(s) 710
Bse3DI GCAATG 3 cut(s) 1041, 1047, 1347
Bse8I GATNNNNATC 2 cut(s) 417, 933
BseBI CCWGG 4 cut(s) 446, 507, 831, 939
BseDI CCNNGG 6 cut(s) 65, 232, 285, 444, 1180, 1209
BseGI GGATG 5 cut(s) 160, 502, 786, 934, 1015
BseJI GATNNNNATC 2 cut(s) 417, 933
BseLI CCNNNNNNNGG 2 cut(s) 796, 938
BseMI GCAATG 3 cut(s) 1041, 1047, 1347
BseMII CTCAG 4 cut(s) 125, 195, 978, 1280
BseNI ACTGG 1 cut(s) 710
BseRI GAGGAG 1 cut(s) 1268
BseSI GKGCMC 1 cut(s) 1284
BseXI GCAGC 7 cut(s) 82, 85, 199, 212, 277, 1182, 1210
BseYI CCCAGC 1 cut(s) 1373
BshFI GGCC 5 cut(s) 64, 284, 381, 990, 1393
BshNI GGYRCC 1 cut(s) 718
BsiHKAI GWGCWC 2 cut(s) 227, 1284
BsiSI CCGG 3 cut(s) 287, 1007, 1182
BslI CCNNNNNNNGG 2 cut(s) 796, 938
BsmAI GTCTC 3 cut(s) 711, 1012, 1437
BsnI GGCC 5 cut(s) 64, 284, 381, 990, 1393
Bsp1286I GDGCHC 2 cut(s) 227, 1284
Bsp143I GATC 9 cut(s) 22, 261, 322, 418, 517, 589, 928, 1000, 1395
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 3 cut(s) 237, 851, 1331
BspANI GGCC 5 cut(s) 64, 284, 381, 990, 1393
BspCNI CTCAG 4 cut(s) 126, 196, 977, 1281
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 3 cut(s) 285, 420, 720
BspMAI CTGCAG 1 cut(s) 1200
BspMI ACCTGC 1 cut(s) 1293
BspPI GGATC 4 cut(s) 413, 426, 525, 923
BspT107I GGYRCC 1 cut(s) 718
BspTI CTTAAG 2 cut(s) 248, 1108
BsrDI GCAATG 3 cut(s) 1041, 1047, 1347
BsrI ACTGG 1 cut(s) 710
BssECI CCNNGG 6 cut(s) 65, 232, 285, 444, 1180, 1209
BssMI GATC 9 cut(s) 22, 261, 322, 418, 517, 589, 928, 1000, 1395
BssNI GRCGYC 1 cut(s) 579
BssT1I CCWWGG 2 cut(s) 65, 232
Bst2UI CCWGG 4 cut(s) 446, 507, 831, 939
Bst4CI ACNGT 1 cut(s) 1279
BstACI GRCGYC 1 cut(s) 579
BstAFI CTTAAG 2 cut(s) 248, 1108
BstC8I GCNNGC 2 cut(s) 549, 885
BstDEI CTNAG 5 cut(s) 134, 204, 964, 1289, 1298
BstDSI CCRYGG 1 cut(s) 65
BstEII GGTNACC 1 cut(s) 1058
BstENI CCTNNNNNAGG 1 cut(s) 936
BstF5I GGATG 5 cut(s) 160, 502, 786, 934, 1015
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 9 cut(s) 25, 264, 325, 421, 520, 592, 931, 1003, 1398
BstMAI GTCTC 3 cut(s) 711, 1012, 1437
BstMBI GATC 9 cut(s) 22, 261, 322, 418, 517, 589, 928, 1000, 1395
BstMWI GCNNNNNNNGC 7 cut(s) 10, 61, 70, 290, 440, 851, 1358
BstNI CCWGG 4 cut(s) 446, 507, 831, 939
BstPAI GACNNNNGTC 1 cut(s) 578
BstPI GGTNACC 1 cut(s) 1058
BstSCI CCNGG 6 cut(s) 285, 444, 505, 829, 937, 1180
BstSFI CTRYAG 2 cut(s) 1084, 1196
BstSLI GKGCMC 1 cut(s) 1284
BstV1I GCAGC 7 cut(s) 82, 85, 199, 212, 277, 1182, 1210
BstV2I GAAGAC 1 cut(s) 578
BstX2I RGATCY 3 cut(s) 418, 517, 1000
BstYI RGATCY 3 cut(s) 418, 517, 1000
BsuI GTATCC 1 cut(s) 623
BsuRI GGCC 5 cut(s) 64, 284, 381, 990, 1393
BtgI CCRYGG 1 cut(s) 65
BtsCI GGATG 5 cut(s) 160, 502, 786, 934, 1015
BtsIMutI CAGTG 1 cut(s) 1284
BveI ACCTGC 1 cut(s) 1293
Cac8I GCNNGC 2 cut(s) 549, 885
CciI TCATGA 1 cut(s) 645
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 3 cut(s) 229, 283, 379
CseI GACGC 1 cut(s) 889
Csp6I GTAC 1 cut(s) 1268
CviAII CATG 6 cut(s) 66, 191, 294, 646, 923, 1133
CviQI GTAC 1 cut(s) 1268
DdeI CTNAG 5 cut(s) 134, 204, 964, 1289, 1298
DpnI GATC 9 cut(s) 24, 263, 324, 420, 519, 591, 930, 1002, 1397
DpnII GATC 9 cut(s) 22, 261, 322, 418, 517, 589, 928, 1000, 1395
EaeI YGGCCR 2 cut(s) 62, 988
Eco130I CCWWGG 2 cut(s) 65, 232
Eco32I GATATC 1 cut(s) 106
Eco47I GGWCC 1 cut(s) 229
Eco47III AGCGCT 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 926
Eco91I GGTNACC 1 cut(s) 1058
EcoNI CCTNNNNNAGG 1 cut(s) 936
EcoO65I GGTNACC 1 cut(s) 1058
EcoRII CCWGG 4 cut(s) 444, 505, 829, 937
EcoRV GATATC 1 cut(s) 106
EcoT14I CCWWGG 2 cut(s) 65, 232
ErhI CCWWGG 2 cut(s) 65, 232
FaeI CATG 6 cut(s) 69, 194, 297, 649, 926, 1136
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FatI CATG 6 cut(s) 65, 190, 293, 645, 922, 1132
FauNDI CATATG 1 cut(s) 889
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 8 cut(s) 71, 99, 188, 201, 291, 852, 1196, 1199
FokI GGATG 5 cut(s) 167, 489, 773, 921, 1022
Fsp4HI GCNGC 8 cut(s) 71, 99, 188, 201, 291, 852, 1196, 1199
FspBI CTAG 2 cut(s) 47, 747
GlaI GCGC 1 cut(s) 74
GluI GCNGC 8 cut(s) 71, 99, 188, 201, 291, 852, 1196, 1199
GsaI CCCAGC 1 cut(s) 1377
GsuI CTGGAG 2 cut(s) 528, 693
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 5 cut(s) 64, 284, 381, 990, 1393
HapII CCGG 3 cut(s) 287, 1007, 1182
HgaI GACGC 1 cut(s) 889
HhaI GCGC 1 cut(s) 75
Hin1I GRCGYC 1 cut(s) 579
Hin1II CATG 6 cut(s) 69, 194, 297, 649, 926, 1136
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 1359
HinfI GANTC 8 cut(s) 43, 408, 726, 780, 793, 901, 1214, 1314
HpaII CCGG 3 cut(s) 287, 1007, 1182
HphI GGTGA 6 cut(s) 160, 404, 443, 482, 812, 1070
Hpy166II GTNNAC 2 cut(s) 451, 1282
Hpy188I TCNGA 6 cut(s) 229, 906, 967, 1078, 1210, 1275
Hpy188III TCNNGA 6 cut(s) 268, 515, 646, 790, 1250, 1440
Hpy8I GTNNAC 2 cut(s) 451, 1282
Hpy99I CGWCG 1 cut(s) 1416
HpyAV CCTTC 2 cut(s) 145, 562
HpyCH4III ACNGT 1 cut(s) 1279
HpyCH4IV ACGT 1 cut(s) 579
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 61, 70, 290, 440, 851, 1358
HpyF3I CTNAG 5 cut(s) 134, 204, 964, 1289, 1298
HpySE526I ACGT 1 cut(s) 579
Hsp92I GRCGYC 1 cut(s) 579
Hsp92II CATG 6 cut(s) 69, 194, 297, 649, 926, 1136
HspAI GCGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 9 cut(s) 22, 261, 322, 418, 517, 589, 928, 1000, 1395
LmnI GCTCC 2 cut(s) 80, 334
Lsp1109I GCAGC 7 cut(s) 82, 85, 199, 212, 277, 1182, 1210
LweI GCATC 4 cut(s) 145, 337, 649, 1082
MaeI CTAG 2 cut(s) 47, 747
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 4 cut(s) 601, 742, 1058, 1186
MalI GATC 9 cut(s) 24, 263, 324, 420, 519, 591, 930, 1002, 1397
MboI GATC 9 cut(s) 22, 261, 322, 418, 517, 589, 928, 1000, 1395
MboII GAAGA 7 cut(s) 172, 220, 235, 583, 919, 1209, 1367
MfeI CAATTG 1 cut(s) 438
MflI RGATCY 3 cut(s) 418, 517, 1000
MhlI GDGCHC 2 cut(s) 227, 1284
MlsI TGGCCA 1 cut(s) 990
MluNI TGGCCA 1 cut(s) 990
MlyI GAGTC 1 cut(s) 720
MmeI TCCRAC 1 cut(s) 345
Mox20I TGGCCA 1 cut(s) 990
MroXI GAANNNNTTC 1 cut(s) 914
MscI TGGCCA 1 cut(s) 990
MseI TTAA 5 cut(s) 249, 1109, 1142, 1224, 1382
Msp20I TGGCCA 1 cut(s) 990
MspA1I CMGCKG 2 cut(s) 133, 203
MspCI CTTAAG 2 cut(s) 248, 1108
MspI CCGG 3 cut(s) 287, 1007, 1182
MspR9I CCNGG 6 cut(s) 287, 446, 507, 831, 939, 1182
MunI CAATTG 1 cut(s) 438
MvaI CCWGG 4 cut(s) 446, 507, 831, 939
MwoI GCNNNNNNNGC 7 cut(s) 10, 61, 70, 290, 440, 851, 1358
NciI CCSGG 2 cut(s) 287, 1182
NcoI CCATGG 1 cut(s) 65
NdeI CATATG 1 cut(s) 889
NdeII GATC 9 cut(s) 22, 261, 322, 418, 517, 589, 928, 1000, 1395
NlaIII CATG 6 cut(s) 69, 194, 297, 649, 926, 1136
NlaIV GGNNCC 3 cut(s) 285, 420, 720
NmuCI GTSAC 3 cut(s) 742, 1058, 1186
PagI TCATGA 1 cut(s) 645
PaqCI CACCTGC 1 cut(s) 1293
PcsI WCGNNNNNNNCGW 1 cut(s) 730
PdmI GAANNNNTTC 1 cut(s) 914
PfeI GAWTC 7 cut(s) 43, 408, 780, 793, 901, 1214, 1314
PfoI TCCNGGA 2 cut(s) 505, 937
PkrI GCNGC 8 cut(s) 72, 100, 189, 202, 292, 853, 1197, 1200
PleI GAGTC 1 cut(s) 720
PpsI GAGTC 1 cut(s) 720
PshAI GACNNNNGTC 1 cut(s) 578
Psp6I CCWGG 4 cut(s) 444, 505, 829, 937
PspEI GGTNACC 1 cut(s) 1058
PspFI CCCAGC 1 cut(s) 1373
PspGI CCWGG 4 cut(s) 444, 505, 829, 937
PspN4I GGNNCC 3 cut(s) 285, 420, 720
PspPI GGNCC 3 cut(s) 229, 283, 379
PstI CTGCAG 1 cut(s) 1200
PsuI RGATCY 3 cut(s) 418, 517, 1000
PvuII CAGCTG 2 cut(s) 133, 203
RsaI GTAC 1 cut(s) 1269
RsaNI GTAC 1 cut(s) 1268
SaqAI TTAA 5 cut(s) 249, 1109, 1142, 1224, 1382
SatI GCNGC 8 cut(s) 71, 99, 188, 201, 291, 852, 1196, 1199
Sau3AI GATC 9 cut(s) 22, 261, 322, 418, 517, 589, 928, 1000, 1395
Sau96I GGNCC 3 cut(s) 229, 283, 379
SchI GAGTC 1 cut(s) 720
ScrFI CCNGG 6 cut(s) 287, 446, 507, 831, 939, 1182
SduI GDGCHC 2 cut(s) 227, 1284
SfaNI GCATC 4 cut(s) 145, 337, 649, 1082
SfcI CTRYAG 2 cut(s) 1084, 1196
SinI GGWCC 1 cut(s) 229
SmlI CTYRAG 2 cut(s) 248, 1108
SmoI CTYRAG 2 cut(s) 248, 1108
SsiI CCGC 3 cut(s) 237, 851, 1331
SspMI CTAG 2 cut(s) 47, 747
StyD4I CCNGG 6 cut(s) 285, 444, 505, 829, 937, 1180
StyI CCWWGG 2 cut(s) 65, 232
TaaI ACNGT 1 cut(s) 1279
TaiI ACGT 1 cut(s) 582
TaqI TCGA 4 cut(s) 94, 411, 1427, 1439
TaqII GACCGA 1 cut(s) 545
TatI WGTACW 1 cut(s) 1267
TauI GCSGC 1 cut(s) 854
TfiI GAWTC 7 cut(s) 43, 408, 780, 793, 901, 1214, 1314
Tru1I TTAA 5 cut(s) 249, 1109, 1142, 1224, 1382
Tru9I TTAA 5 cut(s) 249, 1109, 1142, 1224, 1382
TscAI CASTG 1 cut(s) 1284
TseFI GTSAC 3 cut(s) 742, 1058, 1186
TseI GCWGC 7 cut(s) 70, 98, 187, 200, 290, 1195, 1198
Tsp45I GTSAC 3 cut(s) 742, 1058, 1186
TspDTI ATGAA 6 cut(s) 207, 634, 819, 911, 1140, 1149
TspGWI ACGGA 1 cut(s) 1358
TspRI CASTG 1 cut(s) 1284
Vha464I CTTAAG 2 cut(s) 248, 1108
VneI GTGCAC 1 cut(s) 1280
VpaK11BI GGWCC 1 cut(s) 229
XagI CCTNNNNNAGG 1 cut(s) 936
XapI RAATTY 3 cut(s) 194, 584, 635
XcmI CCANNNNNNNNNTGG 1 cut(s) 793
XmnI GAANNNNTTC 1 cut(s) 914
XspI CTAG 2 cut(s) 47, 747
ZraI GACGTC 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.