Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009255

Physical Location & Seq

Genomic Coordinates Forward (+)

20610075 .. 20612445

Gene Structure 2371 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G50650.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1152 bp

ATGGCGAGTAATCAACAACCACCGTCGAATCTACAGACCTACGGTGTACCAATTTACGCCGTCGACTGGATTCTAGAGGAAGCTGTCAGATCCAAAATCACTAAGGATCAGGATGATGATGATGATGATGGATCGTCGTCGTCGTCGTACATCGTTTTATCCGGCGGAGGAGGAGAAGGACGAAGTGGAATACCTAATGTCATCTTAATCTGTCGTGTTGATCTCCACACCAATTCTCTCTCTGAACAACCTATTGGTAGGCGTGTGATTGGCACTGATCTACCTTATCGTATGGCTATTCATCCTCGTCAAGGTGGTCTTATTTGTGCATTGCCTAACAGTTGCAGACTGTTTGATTGGGAAAACATTATAGAGGATGATAATGAGGAAGAGTCAGAGAAAGTTGTTAAAGAGTTAAAAGATGTTGGACAACAGTTGTCTCTCTCGTTTAATCAGGATGGGACTGTTCTAGCTACTGGTGCAGAGGATGGGACTTTGAGGGTTTTTGAATGGCCAAGCATGAAGACTTTACTTAATGAGTCTAAGACACATGCATCAGTTAAAAGCCTAACTTTCAGCGAAAGTGGTAAGTTTCTTGTATCTCTTGGAGCTCCACTTTGTCGGGTTTGGGATGTGAATGCTTCTGCTGCTATTGCCAGTCTATCAAAAGAGAAGGACGAGATGTTTGCCTCGTGTAGATTCTCTGTAGACAATTCAGGCAGTGAAGTTCTTTATGTTGCTGCAAACACTCAACGTGGTGGGAGTATTATAACATGGGATACAACATCATGGAGAAGAAGAAGTTCAAAGCTTATTAAAAGAAACAACTCTATATCTGCCTTTAATGTCTCAGCTGATGGGAAGCTTCTTGCAGTAGGAACCCTCGAAGGAGATGTTTTAATAATTGATTCGACAAAGATGCAAACTAACCAAATCGTCAAGAAAGCTCATCTCGGTTTGGTCACCGCACTGACTTTCTCCCCTGATTCAAGGTGCTTAGTGTCAGTATCTTTTGACTCAAGGGCAAGGCTAACCGTGATCAAACAAAAGGGTGAAAAACGTCGAGTATATCTGTGGGTGGCGGCATTGTTGTTTGTGTTGGTATACGTGGTTTTGTATTATTTGATGGTGGCAATGGGGATCATACATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002790 GO:0003400 GO:0003674 GO:0005085 GO:0005090 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005886 GO:0006810 GO:0006888 GO:0008047 GO:0008104 GO:0008150 GO:0009306 GO:0009987 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016192 GO:0019899 GO:0030176 GO:0030234 GO:0030695 GO:0031090 GO:0031224 GO:0031227 GO:0031984 GO:0032386 GO:0032879 GO:0032940 GO:0033036 GO:0033043 GO:0042175 GO:0042886 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043547 GO:0044087 GO:0044093 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046903 GO:0046907 GO:0048193 GO:0048209 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0060341 GO:0060589 GO:0060627 GO:0060628 GO:0065007 GO:0065009 GO:0071702 GO:0071705 GO:0071944 GO:0090113 GO:0098588 GO:0098772 GO:0098805 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

ANAPC4_WD40 WD40

Protein Analysis

383

Amino Acids

42.08

Weight (kDa)

5.84

Isoelectric Point (pI)

43.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	122 - 221	1.9e-06	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	132 - 237	7.1e-10	WDR5 beta-propeller domain
WD40_Prp19	PF24814	134 - 247	3.6e-11	Prp19 WD40 domain
Beta-prop_IFT140_1st	PF23383	138 - 340	5.5e-07	IFT140 first beta-propeller
Beta-prop_WDR3_1st	PF25173	145 - 234	6.6e-08	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	147 - 343	2.6e-20	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	147 - 335	2.6e-15	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	175 - 333	5.1e-10	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	208 - 342	2.6e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	209 - 343	7.8e-10	WDR3 first beta-propeller domain
Tricorn_2nd	PF26550	238 - 348	3.6e-06	Tricorn protease second beta propeller domain
Beta-prop_WDR5	PF25175	255 - 350	1.4e-07	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	256 - 344	9.6e-06	WDR36/Utp21 second beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0009255)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01470 AT5G50550 AT5G50650
fragaria_vesca	FvH4_4g32580
malus_domestica	MD08G1204700.v1.1 MD13G1024400.v1.1 MD16G1027100.v1.1
prunus_persica	Prupe.1G326100_v2.0.a1 Prupe.1G326100_v2.0.a1
pyrus_communis	pycom13g02270 pycom16g02290
rosa_chinensis	RchiOBHm_Chr4g0441451
rosa_laevigata	RLG00000006091
rosa_multiflora	Rmu_sc0003360.1_g000007 Rmu_sc0011145.1_g000002
rosa_roxburghii	Rroxscaffold_5G00382320
rosa_rugosa	Rorug04G0333800
rosa_samantha	Rh4AG385300 Rh4BG399500 Rh4CG414500 Rh4DG392100
rosa_wichuraiana	Rw4G033280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	770
AccI	GTMKAC	3 cut(s)	63, 708, 1104
AciI	CCGC	3 cut(s)	165, 966, 1082
AclWI	GGATC	4 cut(s)	84, 114, 139, 1148
AcoI	YGGCCR	1 cut(s)	512
AfaI	GTAC	2 cut(s)	48, 149
AfiI	CCNNNNNNNGG	2 cut(s)	66, 311
AgsI	TTSAA	3 cut(s)	509, 807, 990
AjuI	GAANNNNNNNTTGG	2 cut(s)	237, 269
AluBI	AGCT	7 cut(s)	83, 473, 611, 811, 854, 865, 947
AluI	AGCT	7 cut(s)	83, 473, 611, 811, 854, 865, 947
Alw21I	GWGCWC	1 cut(s)	613
Alw26I	GTCTC	2 cut(s)	444, 853
AlwI	GGATC	4 cut(s)	84, 114, 139, 1148
AoxI	GGCC	1 cut(s)	512
ApeKI	GCWGC	2 cut(s)	647, 740
ArsI	GACNNNNNNTTYG	2 cut(s)	668, 700
Asp700I	GAANNNNTTC	1 cut(s)	802
AsuHPI	GGTGA	2 cut(s)	955, 1064
BalI	TGGCCA	1 cut(s)	514
BanII	GRGCYC	1 cut(s)	613
BauI	CACGAG	1 cut(s)	691
BbsI	GAAGAC	1 cut(s)	530
Bbv12I	GWGCWC	1 cut(s)	613
BbvI	GCAGC	2 cut(s)	634, 727
BccI	CCATC	5 cut(s)	122, 452, 482, 851, 1120
BceAI	ACGGC	1 cut(s)	44
BcgI	CGANNNNNNTGC	4 cut(s)	668, 702, 901, 935
BciVI	GTATCC	1 cut(s)	772
BclI	TGATCA	1 cut(s)	1038
BcoDI	GTCTC	2 cut(s)	444, 853
BfaI	CTAG	2 cut(s)	74, 470
BfmI	CTRYAG	2 cut(s)	32, 705
BfuI	GTATCC	1 cut(s)	772
BisI	GCNGC	3 cut(s)	648, 741, 1083
BlsI	GCNGC	3 cut(s)	649, 742, 1084
BmiI	GGNNCC	1 cut(s)	880
BmsI	GCATC	2 cut(s)	563, 909
BpiI	GAAGAC	1 cut(s)	530
BpuEI	CTTGAG	1 cut(s)	1003
BsaAI	YACGTR	1 cut(s)	1108
Bsc4I	CCNNNNNNNGG	2 cut(s)	66, 311
Bse1I	ACTGG	3 cut(s)	71, 481, 657
Bse3DI	GCAATG	2 cut(s)	329, 1140
BseGI	GGATG	6 cut(s)	118, 301, 382, 463, 493, 637
BseLI	CCNNNNNNNGG	2 cut(s)	66, 311
BseMI	GCAATG	2 cut(s)	329, 1140
BseMII	CTCAG	1 cut(s)	864
BseNI	ACTGG	3 cut(s)	71, 481, 657
BseRI	GAGGAG	2 cut(s)	183, 186
BseXI	GCAGC	2 cut(s)	634, 727
BsgI	GTGCAG	1 cut(s)	501
BshFI	GGCC	1 cut(s)	514
BsiHKAI	GWGCWC	1 cut(s)	613
BsiSI	CCGG	1 cut(s)	162
BslFI	GGGAC	2 cut(s)	475, 505
BslI	CCNNNNNNNGG	2 cut(s)	66, 311
BsmAI	GTCTC	2 cut(s)	444, 853
BsmFI	GGGAC	2 cut(s)	475, 505
BsmI	GAATGC	1 cut(s)	643
BsnI	GGCC	1 cut(s)	514
Bsp1286I	GDGCHC	1 cut(s)	613
Bsp143I	GATC	7 cut(s)	89, 106, 131, 220, 277, 1038, 1140
BspACI	CCGC	3 cut(s)	165, 966, 1082
BspANI	GGCC	1 cut(s)	514
BspCNI	CTCAG	1 cut(s)	863
BspLI	GGNNCC	1 cut(s)	880
BspPI	GGATC	4 cut(s)	84, 114, 139, 1148
BsrDI	GCAATG	2 cut(s)	329, 1140
BsrI	ACTGG	3 cut(s)	71, 481, 657
BssMI	GATC	7 cut(s)	89, 106, 131, 220, 277, 1038, 1140
BssNAI	GTATAC	1 cut(s)	1105
BssSI	CACGAG	1 cut(s)	691
Bst1107I	GTATAC	1 cut(s)	1105
Bst2BI	CACGAG	1 cut(s)	691
Bst4CI	ACNGT	7 cut(s)	24, 44, 341, 351, 435, 466, 1036
Bst6I	CTCTTC	1 cut(s)	384
BstBAI	YACGTR	1 cut(s)	1108
BstDEI	CTNAG	4 cut(s)	102, 543, 850, 997
BstEII	GGTNACC	1 cut(s)	961
BstENI	CCTNNNNNAGG	1 cut(s)	309
BstF5I	GGATG	6 cut(s)	118, 301, 382, 463, 493, 637
BstKTI	GATC	7 cut(s)	92, 109, 134, 223, 280, 1041, 1143
BstMAI	GTCTC	2 cut(s)	444, 853
BstMBI	GATC	7 cut(s)	89, 106, 131, 220, 277, 1038, 1140
BstMWI	GCNNNNNNNGC	3 cut(s)	479, 647, 653
BstNSI	RCATGY	1 cut(s)	554
BstPI	GGTNACC	1 cut(s)	961
BstSFI	CTRYAG	2 cut(s)	32, 705
BstV1I	GCAGC	2 cut(s)	634, 727
BstV2I	GAAGAC	1 cut(s)	530
BstX2I	RGATCY	1 cut(s)	89
BstYI	RGATCY	1 cut(s)	89
BstZ17I	GTATAC	1 cut(s)	1105
BsuI	GTATCC	1 cut(s)	772
BsuRI	GGCC	1 cut(s)	514
BtsCI	GGATG	6 cut(s)	118, 301, 382, 463, 493, 637
BtsI	GCAGTG	1 cut(s)	727
BtsIMutI	CAGTG	3 cut(s)	273, 727, 968
Csp6I	GTAC	2 cut(s)	47, 148
CviAII	CATG	4 cut(s)	520, 551, 774, 789
CviQI	GTAC	2 cut(s)	47, 148
DdeI	CTNAG	4 cut(s)	102, 543, 850, 997
DpnI	GATC	7 cut(s)	91, 108, 133, 222, 279, 1040, 1142
DpnII	GATC	7 cut(s)	89, 106, 131, 220, 277, 1038, 1140
EaeI	YGGCCR	1 cut(s)	512
Eam1104I	CTCTTC	1 cut(s)	384
EarI	CTCTTC	1 cut(s)	384
EciI	GGCGGA	1 cut(s)	180
Ecl136II	GAGCTC	1 cut(s)	611
Eco24I	GRGCYC	1 cut(s)	613
Eco53kI	GAGCTC	1 cut(s)	611
Eco91I	GGTNACC	1 cut(s)	961
EcoICRI	GAGCTC	1 cut(s)	611
EcoNI	CCTNNNNNAGG	1 cut(s)	309
EcoO65I	GGTNACC	1 cut(s)	961
EcoT22I	ATGCAT	1 cut(s)	556
EcoT38I	GRGCYC	1 cut(s)	613
FaeI	CATG	4 cut(s)	523, 554, 777, 792
FalI	AAGNNNNNCTT	4 cut(s)	303, 335, 556, 588
FaqI	GGGAC	2 cut(s)	475, 505
FatI	CATG	4 cut(s)	519, 550, 773, 788
FbaI	TGATCA	1 cut(s)	1038
FblI	GTMKAC	3 cut(s)	63, 708, 1104
Fnu4HI	GCNGC	3 cut(s)	648, 741, 1083
FokI	GGATG	6 cut(s)	125, 288, 389, 470, 500, 644
FriOI	GRGCYC	1 cut(s)	613
Fsp4HI	GCNGC	3 cut(s)	648, 741, 1083
FspBI	CTAG	2 cut(s)	74, 470
GluI	GCNGC	3 cut(s)	648, 741, 1083
HaeIII	GGCC	1 cut(s)	514
HapII	CCGG	1 cut(s)	162
Hin1II	CATG	4 cut(s)	523, 554, 777, 792
HincII	GTYRAC	1 cut(s)	64
HindII	GTYRAC	1 cut(s)	64
HindIII	AAGCTT	2 cut(s)	809, 863
HinfI	GANTC	8 cut(s)	28, 70, 392, 539, 699, 908, 986, 1016
HpaII	CCGG	1 cut(s)	162
HphI	GGTGA	2 cut(s)	955, 1064
Hpy166II	GTNNAC	4 cut(s)	47, 64, 709, 1105
Hpy188I	TCNGA	3 cut(s)	89, 244, 397
Hpy188III	TCNNGA	4 cut(s)	74, 110, 455, 940
Hpy8I	GTNNAC	4 cut(s)	47, 64, 709, 1105
Hpy99I	CGWCG	7 cut(s)	28, 65, 139, 142, 145, 148, 1065
HpyAV	CCTTC	3 cut(s)	170, 667, 881
HpyCH4III	ACNGT	7 cut(s)	24, 44, 341, 351, 435, 466, 1036
HpyCH4IV	ACGT	3 cut(s)	754, 1060, 1107
HpyCH4V	TGCA	7 cut(s)	329, 345, 482, 554, 743, 872, 922
HpyF10VI	GCNNNNNNNGC	3 cut(s)	479, 647, 653
HpyF3I	CTNAG	4 cut(s)	102, 543, 850, 997
HpySE526I	ACGT	3 cut(s)	754, 1060, 1107
Hsp92II	CATG	4 cut(s)	523, 554, 777, 792
Ksp22I	TGATCA	1 cut(s)	1038
Kzo9I	GATC	7 cut(s)	89, 106, 131, 220, 277, 1038, 1140
LmnI	GCTCC	2 cut(s)	608, 616
LpnPI	CCDG	8 cut(s)	52, 95, 175, 440, 462, 670, 702, 996
Lsp1109I	GCAGC	2 cut(s)	634, 727
LweI	GCATC	2 cut(s)	563, 909
MaeI	CTAG	2 cut(s)	74, 470
MaeII	ACGT	3 cut(s)	754, 1060, 1107
MaeIII	GTNAC	1 cut(s)	961
MalI	GATC	7 cut(s)	91, 108, 133, 222, 279, 1040, 1142
MboI	GATC	7 cut(s)	89, 106, 131, 220, 277, 1038, 1140
MboII	GAAGA	4 cut(s)	401, 535, 807, 810
MflI	RGATCY	1 cut(s)	89
MhlI	GDGCHC	1 cut(s)	613
MlsI	TGGCCA	1 cut(s)	514
MluCI	AATT	4 cut(s)	51, 232, 712, 903
MluNI	TGGCCA	1 cut(s)	514
MlyI	GAGTC	3 cut(s)	401, 548, 1010
MmeI	TCCRAC	1 cut(s)	406
Mox20I	TGGCCA	1 cut(s)	514
Mph1103I	ATGCAT	1 cut(s)	556
MroXI	GAANNNNTTC	1 cut(s)	802
MscI	TGGCCA	1 cut(s)	514
MseI	TTAA	9 cut(s)	206, 408, 416, 450, 534, 561, 816, 843, 899
Msp20I	TGGCCA	1 cut(s)	514
MspA1I	CMGCKG	1 cut(s)	854
MspI	CCGG	1 cut(s)	162
Mva1269I	GAATGC	1 cut(s)	643
MwoI	GCNNNNNNNGC	3 cut(s)	479, 647, 653
NdeII	GATC	7 cut(s)	89, 106, 131, 220, 277, 1038, 1140
NlaIII	CATG	4 cut(s)	523, 554, 777, 792
NlaIV	GGNNCC	1 cut(s)	880
NmuCI	GTSAC	1 cut(s)	961
NsiI	ATGCAT	1 cut(s)	556
NspI	RCATGY	1 cut(s)	554
PcsI	WCGNNNNNNNCGW	2 cut(s)	140, 143
PctI	GAATGC	1 cut(s)	643
PdmI	GAANNNNTTC	1 cut(s)	802
PfeI	GAWTC	5 cut(s)	28, 70, 699, 908, 986
PkrI	GCNGC	3 cut(s)	649, 742, 1084
PleI	GAGTC	3 cut(s)	400, 547, 1010
PpsI	GAGTC	3 cut(s)	400, 547, 1010
Ppu21I	YACGTR	1 cut(s)	1108
PsiI	TTATAA	1 cut(s)	770
Psp124BI	GAGCTC	1 cut(s)	613
PspEI	GGTNACC	1 cut(s)	961
PspN4I	GGNNCC	1 cut(s)	880
PsuI	RGATCY	1 cut(s)	89
PvuII	CAGCTG	1 cut(s)	854
RsaI	GTAC	2 cut(s)	48, 149
RsaNI	GTAC	2 cut(s)	47, 148
SacI	GAGCTC	1 cut(s)	613
SalI	GTCGAC	1 cut(s)	62
SaqAI	TTAA	9 cut(s)	206, 408, 416, 450, 534, 561, 816, 843, 899
SatI	GCNGC	3 cut(s)	648, 741, 1083
Sau3AI	GATC	7 cut(s)	89, 106, 131, 220, 277, 1038, 1140
SchI	GAGTC	3 cut(s)	401, 548, 1010
SduI	GDGCHC	1 cut(s)	613
SfaNI	GCATC	2 cut(s)	563, 909
SfcI	CTRYAG	2 cut(s)	32, 705
SmlI	CTYRAG	1 cut(s)	1018
SmoI	CTYRAG	1 cut(s)	1018
Sse9I	AATT	4 cut(s)	51, 232, 712, 903
SsiI	CCGC	3 cut(s)	165, 966, 1082
SspMI	CTAG	2 cut(s)	74, 470
SstI	GAGCTC	1 cut(s)	613
TaaI	ACNGT	7 cut(s)	24, 44, 341, 351, 435, 466, 1036
TaiI	ACGT	3 cut(s)	757, 1063, 1110
TaqI	TCGA	5 cut(s)	26, 63, 885, 911, 1063
TasI	AATT	4 cut(s)	51, 232, 712, 903
TauI	GCSGC	1 cut(s)	1085
TfiI	GAWTC	5 cut(s)	28, 70, 699, 908, 986
Tru1I	TTAA	9 cut(s)	206, 408, 416, 450, 534, 561, 816, 843, 899
Tru9I	TTAA	9 cut(s)	206, 408, 416, 450, 534, 561, 816, 843, 899
TscAI	CASTG	3 cut(s)	280, 727, 975
TseFI	GTSAC	1 cut(s)	961
TseI	GCWGC	2 cut(s)	647, 740
Tsp45I	GTSAC	1 cut(s)	961
TspDTI	ATGAA	2 cut(s)	290, 536
TspRI	CASTG	3 cut(s)	280, 727, 975
XagI	CCTNNNNNAGG	1 cut(s)	309
XbaI	TCTAGA	1 cut(s)	73
XceI	RCATGY	1 cut(s)	554
XmiI	GTMKAC	3 cut(s)	63, 708, 1104
XmnI	GAANNNNTTC	1 cut(s)	802
XspI	CTAG	2 cut(s)	74, 470
Zsp2I	ATGCAT	1 cut(s)	556

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.