Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0009255

Physical Location & Seq

Genomic Coordinates Reverse (-)

1910476 .. 1913586

Gene Structure 3111 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1027100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1215 bp

ATGGACCTTCCCAACAACTACCACAAGTACGGAGTCCCTTTCTACGCCGCCGCTTGGCTCCCCGGCCTCGGCAAATCCAAACGACCCGACCAGGATCAAGACGACGCCGGCGACGACAAAAAGGAAACCGAGACTCAGACGCAGAAGACTGAGACCCAGACTGAATTAGCCCAATTCGATTCCAACCAAAACCACGTCGTTTTCTCCGGCGGTGGCGGAGAGGGCCGCAGCGGCGTCCCAAACGCCATCGTCGTCGCCCAATTCGATCCCGCCTCCGTTTCTCTCTCCAGCCAGCCGGTGGCTAAGCTCAACACCGGCTCTGCTTTGCCGTATAGAATGGCGGTTCACCCCGCCGGTAGCGGTCTCGTTTGCTCCTTCCCTGATAGTTGCAGGTGGTTTGAGTTGGAGGAAGAAAGCAACGAAGTTCCTAAACTGGCTTTGAGGCAGTCTGAGAGAGTGCTGGAGCAATTGCAAGATGTTGGACAACAGTTAGCCTTGGCTTTCGACAAAGACGGTTCCTTGCTTGCTGCTGGAGGCGAGGATGGCAAGTTAAGGGTTTTCAAGTGGCCTAGCATGGAGATTATTCTCGACGAAGATAAAGCTCATACCACAATGAAGCAATTAGATTTCAGCCCTGATGGGAAATTTCTCGTCTCCTTAGGAGATAGAGGCCCTGGTAGGGTGTGGGATGTAACTTCATCTACCGTTGTAGCTTCTCTACCACCAGAAAAGGATGAAGTCTTTTGCGGTTGCAGATTTTCTGTAACTGATGATGGGGGTCATATTTTATATTTTGCAGATAAATCGGGAAGCATTGTAACATGGAACACGACCACTTGGAAGAGGATTGGATCAAAGACTATTAGTAAAGACATTATTTCCGCCTTTGATGTTTCAGCTGACGGAAAACTCCTTGCATGTGGGACAACTGGTGGAGATCTTGTGATATTAAATCCAACCAGAATGCGGATTCATAAAGTGGTTAAGAAAGCACACATGGGCTTTGTAACTGCATTGAGTTTCTCTCATGATTCAAGAGCTTTGGCTTCGGCATCCCTGGATTCAAGTGCAAGGGTGTCCACACTTGAGGAGGACAAGAAGAAAAATGGAGGAGTGTTGAGCTTATGGGTCATCATACTCATCATTCTGATAGCCATTGCTGCATATTTCCAGATGAATCCTGACAAGTTGCCTTCGGCGTTGAAAGGATTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002790 GO:0003400 GO:0003674 GO:0005085 GO:0005090 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005789 GO:0005886 GO:0006810 GO:0006888 GO:0008047 GO:0008104 GO:0008150 GO:0009306 GO:0009987 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016192 GO:0019899 GO:0030176 GO:0030234 GO:0030695 GO:0031090 GO:0031224 GO:0031227 GO:0031984 GO:0032386 GO:0032879 GO:0032940 GO:0033036 GO:0033043 GO:0042175 GO:0042886 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043547 GO:0044087 GO:0044093 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046903 GO:0046907 GO:0048193 GO:0048209 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051641 GO:0051649 GO:0060341 GO:0060589 GO:0060627 GO:0060628 GO:0065007 GO:0065009 GO:0071702 GO:0071705 GO:0071944 GO:0090113 GO:0098588 GO:0098772 GO:0098805 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

ANAPC4_WD40 WD40

Protein Analysis

405

Amino Acids

43.77

Weight (kDa)

5.57

Isoelectric Point (pI)

41.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_Prp19	PF24814	113 - 284	6.9e-09	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	130 - 244	6.6e-09	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	147 - 314	1.2e-11	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	165 - 366	1.2e-16	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	165 - 350	2.1e-16	WDHD1 first WD40 domain
WDR55	PF24796	165 - 349	1.9e-08	WDR55
Beta-prop_EML	PF23409	196 - 356	2.6e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2	PF23414	227 - 362	2.3e-10	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	229 - 363	4.6e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR19_1st	PF23389	257 - 367	2.1e-06	WDR19 first beta-propeller
Beta-prop_THOC3	PF25174	260 - 365	4.4e-09	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	271 - 362	8.3e-07	WDR3 first beta-propeller domain
Beta-prop_Vps41	PF23411	286 - 367	3.8e-06	Vps42 beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0009255)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01470 AT5G50550 AT5G50650
fragaria_vesca	FvH4_4g32580
malus_domestica	MD08G1204700.v1.1 MD13G1024400.v1.1 MD16G1027100.v1.1
prunus_persica	Prupe.1G326100_v2.0.a1 Prupe.1G326100_v2.0.a1
pyrus_communis	pycom13g02270 pycom16g02290
rosa_chinensis	RchiOBHm_Chr4g0441451
rosa_laevigata	RLG00000006091
rosa_multiflora	Rmu_sc0003360.1_g000007 Rmu_sc0011145.1_g000002
rosa_roxburghii	Rroxscaffold_5G00382320
rosa_rugosa	Rorug04G0333800
rosa_samantha	Rh4AG385300 Rh4BG399500 Rh4CG414500 Rh4DG392100
rosa_wichuraiana	Rw4G033280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	381
Acc36I	ACCTGC	1 cut(s)	381
AccB7I	CCANNNNNTGG	1 cut(s)	298
AclWI	GGATC	3 cut(s)	102, 260, 859
AcsI	RAATTY	1 cut(s)	644
AcyI	GRCGYC	2 cut(s)	105, 234
AfaI	GTAC	1 cut(s)	29
AfiI	CCNNNNNNNGG	5 cut(s)	54, 68, 298, 679, 966
AgsI	TTSAA	4 cut(s)	562, 1035, 1065, 1204
AjiI	CACGTC	1 cut(s)	196
AjnI	CCWGG	3 cut(s)	90, 673, 1056
AluBI	AGCT	6 cut(s)	307, 602, 713, 899, 1040, 1122
AluI	AGCT	6 cut(s)	307, 602, 713, 899, 1040, 1122
Alw26I	GTCTC	4 cut(s)	125, 146, 368, 658
AlwI	GGATC	3 cut(s)	102, 260, 859
AoxI	GGCC	4 cut(s)	64, 223, 566, 670
ApeKI	GCWGC	3 cut(s)	228, 527, 1160
ApoI	RAATTY	1 cut(s)	644
AspS9I	GGNCC	3 cut(s)	4, 223, 671
AsuC2I	CCSGG	1 cut(s)	63
AsuHPI	GGTGA	1 cut(s)	338
AvaII	GGWCC	1 cut(s)	4
AxyI	CCTNAGG	1 cut(s)	658
BbsI	GAAGAC	1 cut(s)	152
BbvI	GCAGC	3 cut(s)	240, 514, 1147
BccI	CCATC	4 cut(s)	254, 536, 632, 767
BceAI	ACGGC	1 cut(s)	313
BcgI	CGANNNNNNTGC	2 cut(s)	786, 820
BciT130I	CCWGG	3 cut(s)	92, 675, 1058
BcnI	CCSGG	1 cut(s)	63
BcoDI	GTCTC	4 cut(s)	125, 146, 368, 658
BfaI	CTAG	1 cut(s)	570
BfuAI	ACCTGC	1 cut(s)	381
BglI	GCCNNNNNGGC	1 cut(s)	231
BglII	AGATCT	1 cut(s)	937
BisI	GCNGC	7 cut(s)	48, 51, 226, 229, 232, 528, 1161
BlpI	GCTNAGC	1 cut(s)	303
BlsI	GCNGC	7 cut(s)	49, 52, 227, 230, 233, 529, 1162
Bme1390I	CCNGG	4 cut(s)	63, 92, 675, 1058
Bme18I	GGWCC	1 cut(s)	4
BmgBI	CACGTC	1 cut(s)	196
BmgT120I	GGNCC	3 cut(s)	4, 223, 671
BmiI	GGNNCC	2 cut(s)	59, 517
BmrFI	CCNGG	4 cut(s)	63, 92, 675, 1058
BmsI	GCATC	1 cut(s)	1061
BpiI	GAAGAC	1 cut(s)	152
BplI	GAGNNNNNCTC	2 cut(s)	1009, 1041
BpmI	CTGGAG	3 cut(s)	271, 482, 552
Bpu1102I	GCTNAGC	1 cut(s)	303
BpuEI	CTTGAG	1 cut(s)	1106
BpuMI	CCSGG	1 cut(s)	63
BsaHI	GRCGYC	2 cut(s)	105, 234
BsaI	GGTCTC	2 cut(s)	146, 368
BsaJI	CCNNGG	5 cut(s)	61, 67, 495, 673, 1056
BsaXI	ACNNNNNCTCC	2 cut(s)	927, 957
Bsc4I	CCNNNNNNNGG	5 cut(s)	54, 68, 298, 679, 966
Bse118I	RCCGGY	4 cut(s)	107, 295, 314, 353
Bse1I	ACTGG	2 cut(s)	438, 934
Bse21I	CCTNAGG	1 cut(s)	658
Bse3DI	GCAATG	1 cut(s)	1155
BseBI	CCWGG	3 cut(s)	92, 675, 1058
BseDI	CCNNGG	5 cut(s)	61, 67, 495, 673, 1056
BseGI	GGATG	4 cut(s)	547, 694, 739, 1052
BseLI	CCNNNNNNNGG	5 cut(s)	54, 68, 298, 679, 966
BseMI	GCAATG	1 cut(s)	1155
BseMII	CTCAG	3 cut(s)	141, 149, 441
BseNI	ACTGG	2 cut(s)	438, 934
BseRI	GAGGAG	2 cut(s)	1103, 1125
BseXI	GCAGC	3 cut(s)	240, 514, 1147
BshFI	GGCC	4 cut(s)	66, 225, 568, 672
BsiSI	CCGG	6 cut(s)	63, 108, 207, 296, 315, 354
BslFI	GGGAC	3 cut(s)	20, 221, 937
BslI	CCNNNNNNNGG	5 cut(s)	54, 68, 298, 679, 966
BsmAI	GTCTC	4 cut(s)	125, 146, 368, 658
BsmBI	CGTCTC	1 cut(s)	658
BsmFI	GGGAC	3 cut(s)	20, 221, 937
BsmI	GAATGC	1 cut(s)	969
BsnI	GGCC	4 cut(s)	66, 225, 568, 672
Bso31I	GGTCTC	2 cut(s)	146, 368
Bsp143I	GATC	4 cut(s)	94, 265, 851, 937
Bsp1720I	GCTNAGC	1 cut(s)	303
BspANI	GGCC	4 cut(s)	66, 225, 568, 672
BspCNI	CTCAG	3 cut(s)	142, 148, 442
BspHI	TCATGA	1 cut(s)	1027
BspLI	GGNNCC	2 cut(s)	59, 517
BspMI	ACCTGC	1 cut(s)	381
BspPI	GGATC	3 cut(s)	102, 260, 859
BspTNI	GGTCTC	2 cut(s)	146, 368
BsrDI	GCAATG	1 cut(s)	1155
BsrFI	RCCGGY	4 cut(s)	107, 295, 314, 353
BsrI	ACTGG	2 cut(s)	438, 934
BssAI	RCCGGY	4 cut(s)	107, 295, 314, 353
BssECI	CCNNGG	5 cut(s)	61, 67, 495, 673, 1056
BssMI	GATC	4 cut(s)	94, 265, 851, 937
BssNI	GRCGYC	2 cut(s)	105, 234
BssT1I	CCWWGG	1 cut(s)	495
Bst2UI	CCWGG	3 cut(s)	92, 675, 1058
Bst4CI	ACNGT	3 cut(s)	489, 515, 706
Bst6I	CTCTTC	1 cut(s)	836
BstACI	GRCGYC	2 cut(s)	105, 234
BstC8I	GCNNGC	3 cut(s)	109, 293, 525
BstDEI	CTNAG	5 cut(s)	135, 150, 303, 450, 658
BstF5I	GGATG	4 cut(s)	547, 694, 739, 1052
BstKTI	GATC	4 cut(s)	97, 268, 854, 940
BstMAI	GTCTC	4 cut(s)	125, 146, 368, 658
BstMBI	GATC	4 cut(s)	94, 265, 851, 937
BstMWI	GCNNNNNNNGC	4 cut(s)	222, 231, 543, 1160
BstNI	CCWGG	3 cut(s)	92, 675, 1058
BstNSI	RCATGY	1 cut(s)	921
BstSCI	CCNGG	4 cut(s)	61, 90, 673, 1056
BstV1I	GCAGC	3 cut(s)	240, 514, 1147
BstV2I	GAAGAC	1 cut(s)	152
BstX2I	RGATCY	1 cut(s)	937
BstYI	RGATCY	1 cut(s)	937
Bsu36I	CCTNAGG	1 cut(s)	658
BsuRI	GGCC	4 cut(s)	66, 225, 568, 672
BtrI	CACGTC	1 cut(s)	196
BtsCI	GGATG	4 cut(s)	547, 694, 739, 1052
BveI	ACCTGC	1 cut(s)	381
Cac8I	GCNNGC	3 cut(s)	109, 293, 525
CciI	TCATGA	1 cut(s)	1027
Cfr10I	RCCGGY	4 cut(s)	107, 295, 314, 353
Cfr13I	GGNCC	3 cut(s)	4, 223, 671
CseI	GACGC	3 cut(s)	113, 148, 223
Csp6I	GTAC	1 cut(s)	28
CviAII	CATG	5 cut(s)	574, 822, 918, 997, 1028
CviQI	GTAC	1 cut(s)	28
DdeI	CTNAG	5 cut(s)	135, 150, 303, 450, 658
DpnI	GATC	4 cut(s)	96, 267, 853, 939
DpnII	GATC	4 cut(s)	94, 265, 851, 937
Eam1104I	CTCTTC	1 cut(s)	836
EarI	CTCTTC	1 cut(s)	836
EciI	GGCGGA	2 cut(s)	231, 871
Eco130I	CCWWGG	1 cut(s)	495
Eco31I	GGTCTC	2 cut(s)	146, 368
Eco47I	GGWCC	1 cut(s)	4
Eco81I	CCTNAGG	1 cut(s)	658
EcoO109I	RGGNCCY	1 cut(s)	671
EcoRII	CCWGG	3 cut(s)	90, 673, 1056
EcoT14I	CCWWGG	1 cut(s)	495
ErhI	CCWWGG	1 cut(s)	495
Esp3I	CGTCTC	1 cut(s)	658
FaeI	CATG	5 cut(s)	577, 825, 921, 1000, 1031
FaqI	GGGAC	3 cut(s)	20, 221, 937
FatI	CATG	5 cut(s)	573, 821, 917, 996, 1027
FauI	CCCGC	2 cut(s)	277, 358
Fnu4HI	GCNGC	7 cut(s)	48, 51, 226, 229, 232, 528, 1161
FokI	GGATG	4 cut(s)	554, 701, 746, 1039
Fsp4HI	GCNGC	7 cut(s)	48, 51, 226, 229, 232, 528, 1161
FspBI	CTAG	1 cut(s)	570
GluI	GCNGC	7 cut(s)	48, 51, 226, 229, 232, 528, 1161
GsuI	CTGGAG	3 cut(s)	271, 482, 552
HaeIII	GGCC	4 cut(s)	66, 225, 568, 672
HapII	CCGG	6 cut(s)	63, 108, 207, 296, 315, 354
HgaI	GACGC	3 cut(s)	113, 148, 223
Hin1I	GRCGYC	2 cut(s)	105, 234
Hin1II	CATG	5 cut(s)	577, 825, 921, 1000, 1031
HinfI	GANTC	7 cut(s)	33, 133, 179, 970, 1031, 1061, 1177
HpaII	CCGG	6 cut(s)	63, 108, 207, 296, 315, 354
HphI	GGTGA	1 cut(s)	338
Hpy166II	GTNNAC	2 cut(s)	346, 1080
Hpy188I	TCNGA	3 cut(s)	138, 451, 1149
Hpy188III	TCNNGA	7 cut(s)	98, 587, 807, 1028, 1035, 1171, 1181
Hpy8I	GTNNAC	2 cut(s)	346, 1080
Hpy99I	CGWCG	6 cut(s)	107, 116, 200, 254, 257, 593
HpyAV	CCTTC	3 cut(s)	17, 385, 1203
HpyCH4III	ACNGT	3 cut(s)	489, 515, 706
HpyCH4IV	ACGT	1 cut(s)	195
HpyCH4V	TGCA	8 cut(s)	390, 472, 753, 797, 917, 1013, 1070, 1163
HpyF10VI	GCNNNNNNNGC	4 cut(s)	222, 231, 543, 1160
HpyF3I	CTNAG	5 cut(s)	135, 150, 303, 450, 658
HpySE526I	ACGT	1 cut(s)	195
Hsp92I	GRCGYC	2 cut(s)	105, 234
Hsp92II	CATG	5 cut(s)	577, 825, 921, 1000, 1031
KroI	GCCGGC	1 cut(s)	107
KroNI	GCCGGC	1 cut(s)	109
Kzo9I	GATC	4 cut(s)	94, 265, 851, 937
LmnI	GCTCC	3 cut(s)	63, 377, 463
Lsp1109I	GCAGC	3 cut(s)	240, 514, 1147
LweI	GCATC	1 cut(s)	1061
MaeI	CTAG	1 cut(s)	570
MaeII	ACGT	1 cut(s)	195
MaeIII	GTNAC	4 cut(s)	691, 763, 817, 1006
MalI	GATC	4 cut(s)	96, 267, 853, 939
MboI	GATC	4 cut(s)	94, 265, 851, 937
MboII	GAAGA	5 cut(s)	157, 422, 605, 853, 1111
MfeI	CAATTG	1 cut(s)	467
MflI	RGATCY	1 cut(s)	937
MluCI	AATT	6 cut(s)	164, 173, 260, 467, 620, 644
MlyI	GAGTC	2 cut(s)	42, 127
MmeI	TCCRAC	4 cut(s)	207, 384, 460, 980
MreI	CGCCGGCG	1 cut(s)	107
MroNI	GCCGGC	1 cut(s)	107
MseI	TTAA	4 cut(s)	551, 950, 984, 1213
MspA1I	CMGCKG	2 cut(s)	231, 899
MspI	CCGG	6 cut(s)	63, 108, 207, 296, 315, 354
MspR9I	CCNGG	4 cut(s)	63, 92, 675, 1058
MunI	CAATTG	1 cut(s)	467
Mva1269I	GAATGC	1 cut(s)	969
MvaI	CCWGG	3 cut(s)	92, 675, 1058
MwoI	GCNNNNNNNGC	4 cut(s)	222, 231, 543, 1160
NaeI	GCCGGC	1 cut(s)	109
NciI	CCSGG	1 cut(s)	63
NdeII	GATC	4 cut(s)	94, 265, 851, 937
NgoMIV	GCCGGC	1 cut(s)	107
NlaIII	CATG	5 cut(s)	577, 825, 921, 1000, 1031
NlaIV	GGNNCC	2 cut(s)	59, 517
NmeAIII	GCCGAG	1 cut(s)	48
NspI	RCATGY	1 cut(s)	921
PagI	TCATGA	1 cut(s)	1027
PaqCI	CACCTGC	1 cut(s)	381
PcsI	WCGNNNNNNNCGW	4 cut(s)	108, 111, 249, 261
PctI	GAATGC	1 cut(s)	969
PdiI	GCCGGC	1 cut(s)	109
PfeI	GAWTC	5 cut(s)	179, 970, 1031, 1061, 1177
PflMI	CCANNNNNTGG	1 cut(s)	298
PkrI	GCNGC	7 cut(s)	49, 52, 227, 230, 233, 529, 1162
PleI	GAGTC	2 cut(s)	41, 127
PpsI	GAGTC	2 cut(s)	41, 127
Psp6I	CCWGG	3 cut(s)	90, 673, 1056
PspGI	CCWGG	3 cut(s)	90, 673, 1056
PspN4I	GGNNCC	2 cut(s)	59, 517
PspPI	GGNCC	3 cut(s)	4, 223, 671
PsuI	RGATCY	1 cut(s)	937
PvuII	CAGCTG	1 cut(s)	899
RsaI	GTAC	1 cut(s)	29
RsaNI	GTAC	1 cut(s)	28
SaqAI	TTAA	4 cut(s)	551, 950, 984, 1213
SatI	GCNGC	7 cut(s)	48, 51, 226, 229, 232, 528, 1161
Sau3AI	GATC	4 cut(s)	94, 265, 851, 937
Sau96I	GGNCC	3 cut(s)	4, 223, 671
SchI	GAGTC	2 cut(s)	42, 127
ScrFI	CCNGG	4 cut(s)	63, 92, 675, 1058
SetI	ASST	9 cut(s)	9, 198, 309, 395, 604, 715, 901, 1042, 1124
SfaNI	GCATC	1 cut(s)	1061
SgrAI	CRCCGGYG	1 cut(s)	107
SinI	GGWCC	1 cut(s)	4
SmlI	CTYRAG	1 cut(s)	1085
SmoI	CTYRAG	1 cut(s)	1085
Sse9I	AATT	6 cut(s)	164, 173, 260, 467, 620, 644
SspMI	CTAG	1 cut(s)	570
StyD4I	CCNGG	4 cut(s)	61, 90, 673, 1056
StyI	CCWWGG	1 cut(s)	495
TaaI	ACNGT	3 cut(s)	489, 515, 706
TaiI	ACGT	1 cut(s)	198
TaqI	TCGA	4 cut(s)	177, 264, 504, 588
TasI	AATT	6 cut(s)	164, 173, 260, 467, 620, 644
TauI	GCSGC	4 cut(s)	50, 53, 228, 234
TfiI	GAWTC	5 cut(s)	179, 970, 1031, 1061, 1177
Tru1I	TTAA	4 cut(s)	551, 950, 984, 1213
Tru9I	TTAA	4 cut(s)	551, 950, 984, 1213
TseI	GCWGC	3 cut(s)	228, 527, 1160
TspDTI	ATGAA	5 cut(s)	629, 687, 750, 962, 1190
TspGWI	ACGGA	3 cut(s)	45, 265, 918
Van91I	CCANNNNNTGG	1 cut(s)	298
VpaK11BI	GGWCC	1 cut(s)	4
XapI	RAATTY	1 cut(s)	644
XceI	RCATGY	1 cut(s)	921
XcmI	CCANNNNNNNNNTGG	1 cut(s)	295
XspI	CTAG	1 cut(s)	570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.