AT5G52900

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010886
Physical Location & Seq
Reverse (-)
21452442 .. 21453980
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52900.1

Sequence Viewer

Length: 708 bp
ATGGAAGAAGGTAAGCAAGAAGCTTCAATGCCACTAGTTCCAATTGACAGCTTCTCTTACAGTTGGTTAGTTAACTTTCCTTCTTTAGAAGCTACCATTGATGATCATCATCAAACATACGAAGATTCATCTTCTTCTTCTTCCTTCATTGAGATGGATCCAAGATTGCCTCCTTCTAGAAGATTCTTCATCAAAACATCTCATGAAAGTAGCTTCAAGTTCGATAACTTCGTCTCTTTCTCCGACGAAGATCACTCTTTAGTTCACGCCGACGAGCTCTTCCGTGACGGCTACGTTATGCCTTATCGATTAAAGCCCACGTCCGCGGCTACCGAGGAAGAATCTGAGCCGTTGGATACAACAACATCGGAGAAGATTGATACACGTGGCCTTAACAGCAAGCCTTCTCCTACTTCTTCAAGAAAGCTGAGGAGAGTGTCCAAATGGGTTTTGCTGAAGTATCTTGATTTCTTGACGCCATTGTGTAAGAGATTAAGGAGATGTAGATCCGCTGTAACAACGGGAAGTATCGGTATGGATTCGAGGATCCGTGTAACGACTTCGTGTAGGAGCAGAGTTTATTCTGATGAAATGACGTCGTCGCCAAGAATAAGTGTTGCTGATGATTATTATTGGAGAAGGTCTTGTGACTCTGAGAGCTCTATATACGAAGCCGTTCTTCATTGCAAGAAATCATTCGAGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

235

Amino Acids

27.07

Weight (kDa)

6.1

Isoelectric Point (pI)

64.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 599
AccII CGCG 1 cut(s) 326
AciI CCGC 3 cut(s) 324, 326, 510
AclWI GGATC 5 cut(s) 152, 165, 501, 541, 554
AcuI CTGAAG 1 cut(s) 476
AcvI CACGTG 1 cut(s) 386
AcyI GRCGYC 2 cut(s) 476, 596
AflIII ACRYGT 1 cut(s) 383
AgsI TTSAA 3 cut(s) 27, 217, 420
AhlI ACTAGT 1 cut(s) 34
AjiI CACGTC 1 cut(s) 321
AluBI AGCT 7 cut(s) 23, 51, 92, 213, 277, 427, 660
AluI AGCT 7 cut(s) 23, 51, 92, 213, 277, 427, 660
Alw21I GWGCWC 2 cut(s) 279, 662
Alw26I GTCTC 1 cut(s) 238
AlwI GGATC 5 cut(s) 152, 165, 501, 541, 554
AoxI GGCC 1 cut(s) 388
Asp700I GAANNNNTTC 2 cut(s) 675, 695
BamHI GGATCC 2 cut(s) 157, 546
BanII GRGCYC 2 cut(s) 279, 662
BbrPI CACGTG 1 cut(s) 386
Bbv12I GWGCWC 2 cut(s) 279, 662
BbvCI CCTCAGC 1 cut(s) 428
BccI CCATC 1 cut(s) 148
BceAI ACGGC 3 cut(s) 304, 334, 659
BciVI GTATCC 1 cut(s) 349
BclI TGATCA 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 238
BcuI ACTAGT 1 cut(s) 34
BfaI CTAG 2 cut(s) 35, 177
BfuI GTATCC 1 cut(s) 349
BisI GCNGC 1 cut(s) 327
BlsI GCNGC 1 cut(s) 328
BmgBI CACGTC 1 cut(s) 321
BmiI GGNNCC 2 cut(s) 159, 548
Bpu10I CCTNAGC 1 cut(s) 428
Bsa29I ATCGAT 1 cut(s) 307
BsaAI YACGTR 1 cut(s) 386
BsaBI GATNNNNATC 3 cut(s) 105, 108, 505
BsaHI GRCGYC 2 cut(s) 476, 596
BsaJI CCNNGG 2 cut(s) 324, 333
Bse3DI GCAATG 1 cut(s) 682
Bse8I GATNNNNATC 3 cut(s) 105, 108, 505
BseCI ATCGAT 1 cut(s) 307
BseDI CCNNGG 2 cut(s) 324, 333
BseJI GATNNNNATC 3 cut(s) 105, 108, 505
BseMI GCAATG 1 cut(s) 682
BseMII CTCAG 3 cut(s) 336, 419, 645
BseRI GAGGAG 1 cut(s) 445
Bsh1236I CGCG 1 cut(s) 326
BshFI GGCC 1 cut(s) 390
BshVI ATCGAT 1 cut(s) 307
BsiHKAI GWGCWC 2 cut(s) 279, 662
BsmAI GTCTC 1 cut(s) 238
BsmBI CGTCTC 1 cut(s) 238
BsnI GGCC 1 cut(s) 390
Bsp1286I GDGCHC 2 cut(s) 279, 662
Bsp143I GATC 5 cut(s) 103, 157, 250, 506, 546
BspACI CCGC 3 cut(s) 324, 326, 510
BspANI GGCC 1 cut(s) 390
BspCNI CTCAG 3 cut(s) 337, 420, 646
BspDI ATCGAT 1 cut(s) 307
BspFNI CGCG 1 cut(s) 326
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 2 cut(s) 159, 548
BspPI GGATC 5 cut(s) 152, 165, 501, 541, 554
BspQI GCTCTTC 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 682
BssECI CCNNGG 2 cut(s) 324, 333
BssMI GATC 5 cut(s) 103, 157, 250, 506, 546
BssNI GRCGYC 2 cut(s) 476, 596
Bst4CI ACNGT 1 cut(s) 62
Bst6I CTCTTC 1 cut(s) 284
BstACI GRCGYC 2 cut(s) 476, 596
BstBAI YACGTR 1 cut(s) 386
BstC8I GCNNGC 1 cut(s) 401
BstDEI CTNAG 3 cut(s) 345, 428, 654
BstDSI CCRYGG 1 cut(s) 324
BstFNI CGCG 1 cut(s) 326
BstKTI GATC 5 cut(s) 106, 160, 253, 509, 549
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 5 cut(s) 103, 157, 250, 506, 546
BstMWI GCNNNNNNNGC 1 cut(s) 396
BstUI CGCG 1 cut(s) 326
BstX2I RGATCY 3 cut(s) 157, 506, 546
BstYI RGATCY 3 cut(s) 157, 506, 546
Bsu15I ATCGAT 1 cut(s) 307
BsuI GTATCC 1 cut(s) 349
BsuRI GGCC 1 cut(s) 390
BsuTUI ATCGAT 1 cut(s) 307
BtgI CCRYGG 1 cut(s) 324
BtrI CACGTC 1 cut(s) 321
Cac8I GCNNGC 1 cut(s) 401
CciI TCATGA 1 cut(s) 202
Cfr42I CCGCGG 1 cut(s) 327
ClaI ATCGAT 1 cut(s) 307
CseI GACGC 1 cut(s) 484
CviAII CATG 1 cut(s) 203
DdeI CTNAG 3 cut(s) 345, 428, 654
DpnI GATC 5 cut(s) 105, 159, 252, 508, 548
DpnII GATC 5 cut(s) 103, 157, 250, 506, 546
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Ecl136II GAGCTC 2 cut(s) 277, 660
Eco24I GRGCYC 2 cut(s) 279, 662
Eco53kI GAGCTC 2 cut(s) 277, 660
Eco57I CTGAAG 1 cut(s) 476
Eco72I CACGTG 1 cut(s) 386
EcoICRI GAGCTC 2 cut(s) 277, 660
EcoT38I GRGCYC 2 cut(s) 279, 662
Esp3I CGTCTC 1 cut(s) 238
FaeI CATG 1 cut(s) 206
FaiI YATR 6 cut(s) 118, 204, 299, 536, 665, 667
FalI AAGNNNNNCTT 2 cut(s) 663, 695
FatI CATG 1 cut(s) 202
FbaI TGATCA 1 cut(s) 103
Fnu4HI GCNGC 1 cut(s) 327
FriOI GRGCYC 2 cut(s) 279, 662
Fsp4HI GCNGC 1 cut(s) 327
FspBI CTAG 2 cut(s) 35, 177
GluI GCNGC 1 cut(s) 327
HaeIII GGCC 1 cut(s) 390
HgaI GACGC 1 cut(s) 484
Hin1I GRCGYC 2 cut(s) 476, 596
Hin1II CATG 1 cut(s) 206
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 21
HinfI GANTC 5 cut(s) 125, 183, 341, 539, 650
HpaI GTTAAC 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 73, 265
Hpy188I TCNGA 5 cut(s) 244, 346, 370, 586, 655
Hpy188III TCNNGA 6 cut(s) 177, 203, 420, 464, 472, 700
Hpy8I GTNNAC 2 cut(s) 73, 265
Hpy99I CGWCG 4 cut(s) 248, 275, 601, 604
HpyAV CCTTC 5 cut(s) 90, 154, 183, 414, 633
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4IV ACGT 4 cut(s) 294, 320, 385, 596
HpyCH4V TGCA 1 cut(s) 687
HpyF10VI GCNNNNNNNGC 1 cut(s) 396
HpyF3I CTNAG 3 cut(s) 345, 428, 654
HpySE526I ACGT 4 cut(s) 294, 320, 385, 596
Hsp92I GRCGYC 2 cut(s) 476, 596
Hsp92II CATG 1 cut(s) 206
Ksp22I TGATCA 1 cut(s) 103
KspAI GTTAAC 1 cut(s) 73
KspI CCGCGG 1 cut(s) 327
Kzo9I GATC 5 cut(s) 103, 157, 250, 506, 546
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 1 cut(s) 570
MaeI CTAG 2 cut(s) 35, 177
MaeII ACGT 4 cut(s) 294, 320, 385, 596
MaeIII GTNAC 4 cut(s) 284, 514, 553, 647
MalI GATC 5 cut(s) 105, 159, 252, 508, 548
MboI GATC 5 cut(s) 103, 157, 250, 506, 546
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 3 cut(s) 157, 506, 546
MhlI GDGCHC 2 cut(s) 279, 662
MluCI AATT 1 cut(s) 42
MlyI GAGTC 1 cut(s) 644
MmeI TCCRAC 2 cut(s) 267, 333
MnlI CCTC 4 cut(s) 180, 328, 423, 537
MroXI GAANNNNTTC 2 cut(s) 675, 695
MseI TTAA 4 cut(s) 72, 311, 393, 494
MslI CAYNNNNRTG 1 cut(s) 152
MspA1I CMGCKG 2 cut(s) 326, 512
MunI CAATTG 1 cut(s) 42
MvnI CGCG 1 cut(s) 326
MwoI GCNNNNNNNGC 1 cut(s) 396
NdeII GATC 5 cut(s) 103, 157, 250, 506, 546
NlaIII CATG 1 cut(s) 206
NlaIV GGNNCC 2 cut(s) 159, 548
NmuCI GTSAC 2 cut(s) 284, 647
PagI TCATGA 1 cut(s) 202
PciSI GCTCTTC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PdmI GAANNNNTTC 2 cut(s) 675, 695
PfeI GAWTC 4 cut(s) 125, 183, 341, 539
PflFI GACNNNGTC 1 cut(s) 598
PkrI GCNGC 1 cut(s) 328
PleI GAGTC 1 cut(s) 644
PmaCI CACGTG 1 cut(s) 386
PmlI CACGTG 1 cut(s) 386
PpsI GAGTC 1 cut(s) 644
Ppu21I YACGTR 1 cut(s) 386
Psp124BI GAGCTC 2 cut(s) 279, 662
PspCI CACGTG 1 cut(s) 386
PspN4I GGNNCC 2 cut(s) 159, 548
PsuI RGATCY 3 cut(s) 157, 506, 546
PsyI GACNNNGTC 1 cut(s) 598
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 2 cut(s) 279, 662
SacII CCGCGG 1 cut(s) 327
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 4 cut(s) 72, 311, 393, 494
SatI GCNGC 1 cut(s) 327
Sau3AI GATC 5 cut(s) 103, 157, 250, 506, 546
SchI GAGTC 1 cut(s) 644
SduI GDGCHC 2 cut(s) 279, 662
Sfr303I CCGCGG 1 cut(s) 327
SgrBI CCGCGG 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 152
SpeI ACTAGT 1 cut(s) 34
Sse9I AATT 1 cut(s) 42
SsiI CCGC 3 cut(s) 324, 326, 510
SspMI CTAG 2 cut(s) 35, 177
SstI GAGCTC 2 cut(s) 279, 662
TaaI ACNGT 1 cut(s) 62
TaiI ACGT 4 cut(s) 297, 323, 388, 599
TaqI TCGA 4 cut(s) 222, 307, 542, 699
TasI AATT 1 cut(s) 42
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 4 cut(s) 125, 183, 341, 539
Tru1I TTAA 4 cut(s) 72, 311, 393, 494
Tru9I TTAA 4 cut(s) 72, 311, 393, 494
TseFI GTSAC 2 cut(s) 284, 647
Tsp45I GTSAC 2 cut(s) 284, 647
TspDTI ATGAA 6 cut(s) 117, 136, 178, 219, 603, 671
TspGWI ACGGA 2 cut(s) 272, 539
Tth111I GACNNNGTC 1 cut(s) 598
XbaI TCTAGA 1 cut(s) 176
XmnI GAANNNNTTC 2 cut(s) 675, 695
XspI CTAG 2 cut(s) 35, 177
ZraI GACGTC 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.