Rroxscaffold_4G00280060

membrane-associated kinase regulator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010886
Physical Location & Seq
Forward (+)
2723551 .. 2724844
1294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00280060.1

Sequence Viewer

Length: 720 bp
ATGGAAACATCTCAGCAACCTCTCTCCATTGAAAGCTTTTCATACAGTTGGTTAGTGAATCTCAAGCCTCCTTCTTTTGAAAGCCTTGACAACTCCCTTAGGACTTCGCTAGACGCATCCGATGAAGCCTCCTTCATTGAGATGGACCCAACAATGCCACCCTCTCAGAGATTCTTTAGGAATTCCCAGCAGGATTTCAAATTTGACTCACAATCTCCTCTCTCTACTCTTGTTCATGCTGATGAGCTCATTTCCAATGGTTATCTTTTGCCACTTTCGGTTGATGACCCATTGAAGAATATGGAGTCATATGATGATTATTATGATCAACCCTCGAATTCCAATACTGCAAACCTTCCAGTCTCTTCATCAAAAGAAGCTGCTCCAACAGAAAATTCTTCTTCAGATTGCTCTAATTCCTTGAGAAGGTGCAGAAGATTGTCAAAACGAATATTTGAGAAGTACTTGGATTTTCTCAGACCGTTGTACAAAAGGATTAGAGGAGGTCATAAAGCAAATCCTAAAGTAGCTGGGAATCATGTTGATAAAAGGTTCCATTCATCGAAGAACAGCAGGGTGTATTCATCAGAAACTGCATCACCGCGAATAAGCGTAGCTTATTCTGCCCCCGATGATTGGCGAAGGTCCTGTGATTCCGAGAGCTCAATATATGAGGCAGTTCTTCATTGCAAAAGATCCATAGGAAACCAAGTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

239

Amino Acids

27.1

Weight (kDa)

6.22

Isoelectric Point (pI)

68.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 604
AciI CCGC 1 cut(s) 602
AclWI GGATC 1 cut(s) 690
AcsI RAATTY 4 cut(s) 181, 200, 337, 394
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 2 cut(s) 464, 488
AfiI CCNNNNNNNGG 2 cut(s) 426, 636
AgsI TTSAA 4 cut(s) 32, 80, 199, 295
AjuI GAANNNNNNNTTGG 2 cut(s) 379, 411
AluBI AGCT 6 cut(s) 36, 247, 380, 530, 617, 663
AluI AGCT 6 cut(s) 36, 247, 380, 530, 617, 663
Alw21I GWGCWC 2 cut(s) 249, 665
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 1 cut(s) 690
AlwNI CAGNNNCTG 1 cut(s) 593
ApeKI GCWGC 1 cut(s) 380
ApoI RAATTY 4 cut(s) 181, 200, 337, 394
AspS9I GGNCC 2 cut(s) 145, 645
AsuHPI GGTGA 1 cut(s) 591
AvaII GGWCC 2 cut(s) 145, 645
AxyI CCTNAGG 1 cut(s) 98
BanII GRGCYC 2 cut(s) 249, 665
Bbv12I GWGCWC 2 cut(s) 249, 665
BbvI GCAGC 1 cut(s) 367
BccI CCATC 1 cut(s) 136
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 1 cut(s) 110
BisI GCNGC 1 cut(s) 381
BlsI GCNGC 1 cut(s) 382
BmcAI AGTACT 1 cut(s) 464
Bme18I GGWCC 2 cut(s) 145, 645
BmgT120I GGNCC 2 cut(s) 145, 645
BmiI GGNNCC 2 cut(s) 147, 554
BmsI GCATC 2 cut(s) 125, 605
BpuEI CTTGAG 2 cut(s) 47, 442
Bsc4I CCNNNNNNNGG 2 cut(s) 426, 636
Bse1I ACTGG 1 cut(s) 359
Bse21I CCTNAGG 1 cut(s) 98
Bse3DI GCAATG 1 cut(s) 685
BseGI GGATG 1 cut(s) 116
BseLI CCNNNNNNNGG 2 cut(s) 426, 636
BseMI GCAATG 1 cut(s) 685
BseMII CTCAG 3 cut(s) 26, 179, 490
BseNI ACTGG 1 cut(s) 359
BseRI GAGGAG 2 cut(s) 207, 516
BseXI GCAGC 1 cut(s) 367
BseYI CCCAGC 2 cut(s) 186, 530
BsgI GTGCAG 1 cut(s) 451
Bsh1236I CGCG 1 cut(s) 604
BsiHKAI GWGCWC 2 cut(s) 249, 665
BslI CCNNNNNNNGG 2 cut(s) 426, 636
BsmAI GTCTC 1 cut(s) 367
Bsp1286I GDGCHC 2 cut(s) 249, 665
Bsp1407I TGTACA 1 cut(s) 486
Bsp143I GATC 2 cut(s) 325, 695
BspACI CCGC 1 cut(s) 602
BspCNI CTCAG 3 cut(s) 25, 178, 489
BspFNI CGCG 1 cut(s) 604
BspLI GGNNCC 2 cut(s) 147, 554
BspPI GGATC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 685
BsrGI TGTACA 1 cut(s) 486
BsrI ACTGG 1 cut(s) 359
BssMI GATC 2 cut(s) 325, 695
Bst4CI ACNGT 2 cut(s) 47, 483
Bst6I CTCTTC 1 cut(s) 370
BstAUI TGTACA 1 cut(s) 486
BstDEI CTNAG 4 cut(s) 12, 98, 165, 476
BstENI CCTNNNNNAGG 1 cut(s) 424
BstF5I GGATG 1 cut(s) 116
BstFNI CGCG 1 cut(s) 604
BstKTI GATC 2 cut(s) 328, 698
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 2 cut(s) 325, 695
BstMWI GCNNNNNNNGC 1 cut(s) 623
BstUI CGCG 1 cut(s) 604
BstV1I GCAGC 1 cut(s) 367
BstX2I RGATCY 1 cut(s) 695
BstYI RGATCY 1 cut(s) 695
Bsu36I CCTNAGG 1 cut(s) 98
BtsCI GGATG 1 cut(s) 116
CaiI CAGNNNCTG 1 cut(s) 593
Cfr13I GGNCC 2 cut(s) 145, 645
CseI GACGC 1 cut(s) 122
Csp6I GTAC 2 cut(s) 463, 487
CviAII CATG 2 cut(s) 236, 539
CviJI RGCY 9 cut(s) 36, 67, 84, 128, 247, 380, 530, 617, 663
CviKI_1 RGCY 9 cut(s) 36, 67, 84, 128, 247, 380, 530, 617, 663
CviQI GTAC 2 cut(s) 463, 487
DdeI CTNAG 4 cut(s) 12, 98, 165, 476
DpnI GATC 2 cut(s) 327, 697
DpnII GATC 2 cut(s) 325, 695
Eam1104I CTCTTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 370
Ecl136II GAGCTC 2 cut(s) 247, 663
Eco24I GRGCYC 2 cut(s) 249, 665
Eco47I GGWCC 2 cut(s) 145, 645
Eco53kI GAGCTC 2 cut(s) 247, 663
Eco57I CTGAAG 1 cut(s) 387
Eco81I CCTNAGG 1 cut(s) 98
EcoICRI GAGCTC 2 cut(s) 247, 663
EcoNI CCTNNNNNAGG 1 cut(s) 424
EcoO109I RGGNCCY 1 cut(s) 645
EcoRI GAATTC 2 cut(s) 181, 337
EcoT38I GRGCYC 2 cut(s) 249, 665
FaeI CATG 2 cut(s) 239, 542
FalI AAGNNNNNCTT 2 cut(s) 601, 633
FatI CATG 2 cut(s) 235, 538
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 1 cut(s) 381
FokI GGATG 1 cut(s) 103
FriOI GRGCYC 2 cut(s) 249, 665
Fsp4HI GCNGC 1 cut(s) 381
FspBI CTAG 1 cut(s) 110
GluI GCNGC 1 cut(s) 381
GsaI CCCAGC 2 cut(s) 190, 534
HgaI GACGC 1 cut(s) 122
Hin1II CATG 2 cut(s) 239, 542
HindIII AAGCTT 1 cut(s) 34
HinfI GANTC 6 cut(s) 58, 171, 206, 305, 535, 653
HphI GGTGA 1 cut(s) 591
Hpy188I TCNGA 6 cut(s) 121, 168, 406, 479, 589, 658
HpyAV CCTTC 5 cut(s) 81, 142, 365, 420, 636
HpyCH4III ACNGT 2 cut(s) 47, 483
HpyCH4V TGCA 4 cut(s) 350, 432, 596, 690
HpyF10VI GCNNNNNNNGC 1 cut(s) 623
HpyF3I CTNAG 4 cut(s) 12, 98, 165, 476
Hsp92II CATG 2 cut(s) 239, 542
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 2 cut(s) 325, 695
LmnI GCTCC 1 cut(s) 388
LpnPI CCDG 6 cut(s) 176, 200, 372, 516, 559, 661
Lsp1109I GCAGC 1 cut(s) 367
LweI GCATC 2 cut(s) 125, 605
MaeI CTAG 1 cut(s) 110
MalI GATC 2 cut(s) 327, 697
MboI GATC 2 cut(s) 325, 695
MboII GAAGA 7 cut(s) 307, 357, 390, 393, 447, 577, 674
MfeI CAATTG 1 cut(s) 715
MflI RGATCY 1 cut(s) 695
MhlI GDGCHC 2 cut(s) 249, 665
MluCI AATT 6 cut(s) 181, 200, 337, 394, 415, 715
MlyI GAGTC 2 cut(s) 200, 314
MmeI TCCRAC 1 cut(s) 410
MnlI CCTC 9 cut(s) 30, 78, 139, 172, 228, 343, 494, 497, 667
MslI CAYNNNNRTG 2 cut(s) 140, 240
MunI CAATTG 1 cut(s) 715
MvnI CGCG 1 cut(s) 604
MwoI GCNNNNNNNGC 1 cut(s) 623
NdeI CATATG 1 cut(s) 310
NdeII GATC 2 cut(s) 325, 695
NlaIII CATG 2 cut(s) 239, 542
NlaIV GGNNCC 2 cut(s) 147, 554
PfeI GAWTC 4 cut(s) 58, 171, 535, 653
PkrI GCNGC 1 cut(s) 382
PleI GAGTC 2 cut(s) 200, 313
PpsI GAGTC 2 cut(s) 200, 313
PpuMI RGGWCCY 1 cut(s) 645
Psp124BI GAGCTC 2 cut(s) 249, 665
Psp5II RGGWCCY 1 cut(s) 645
PspFI CCCAGC 2 cut(s) 186, 530
PspN4I GGNNCC 2 cut(s) 147, 554
PspPI GGNCC 2 cut(s) 145, 645
PspPPI RGGWCCY 1 cut(s) 645
PstNI CAGNNNCTG 1 cut(s) 593
PsuI RGATCY 1 cut(s) 695
RsaI GTAC 2 cut(s) 464, 488
RsaNI GTAC 2 cut(s) 463, 487
RseI CAYNNNNRTG 2 cut(s) 140, 240
SacI GAGCTC 2 cut(s) 249, 665
SatI GCNGC 1 cut(s) 381
Sau3AI GATC 2 cut(s) 325, 695
Sau96I GGNCC 2 cut(s) 145, 645
ScaI AGTACT 1 cut(s) 464
SchI GAGTC 2 cut(s) 200, 314
SduI GDGCHC 2 cut(s) 249, 665
SfaNI GCATC 2 cut(s) 125, 605
SinI GGWCC 2 cut(s) 145, 645
SmiMI CAYNNNNRTG 2 cut(s) 140, 240
SmlI CTYRAG 2 cut(s) 62, 421
SmoI CTYRAG 2 cut(s) 62, 421
Sse9I AATT 6 cut(s) 181, 200, 337, 394, 415, 715
SsiI CCGC 1 cut(s) 602
SspI AATATT 1 cut(s) 453
SspMI CTAG 1 cut(s) 110
SstI GAGCTC 2 cut(s) 249, 665
TaaI ACNGT 2 cut(s) 47, 483
TaqI TCGA 2 cut(s) 335, 563
TasI AATT 6 cut(s) 181, 200, 337, 394, 415, 715
TatI WGTACW 2 cut(s) 462, 486
TfiI GAWTC 4 cut(s) 58, 171, 535, 653
TseI GCWGC 1 cut(s) 380
TspDTI ATGAA 8 cut(s) 30, 124, 138, 224, 357, 549, 573, 674
VpaK11BI GGWCC 2 cut(s) 145, 645
XagI CCTNNNNNAGG 1 cut(s) 424
XapI RAATTY 4 cut(s) 181, 200, 337, 394
XspI CTAG 1 cut(s) 110
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.