Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0012486

Physical Location & Seq

Genomic Coordinates Reverse (-)

21562718 .. 21568023

Gene Structure 5306 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G53170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2421 bp

ATGTCTTCTTCTACTCTTCAAGCTTCTCTTTTTCTTCGTCCTCCTCTCCATACTTCTTCGTTTAAGCTTTACCCTTGTCTATTCTCTTCTTCTTCATTGAGTTTTTGTCCACAATCTCTCTCAAGTTTCTACCGTCTTTCTTCAGTTCTTCACAACTCTCGATTTCGTCCCCTTCCTTGCTCATTGCGTCAAGACAATGTCGCTTCCGATTCTGATTTCATTCCCAAAGACTCCGCTTTTGAGGTTACGGATTCAGCTGAGAGTAATAGATTGGTTTCCGATACGGAGGTTTCGGAATTAGAGACGAATGATAGATTTGTGGGTGGTGAGGAGACGAAAAGTGGAGGAGAAGAAGCGGAGGTTAGTAATGGAGTAACGGAAGGTAAGGAGGAAGATCAGAAGAAGAGCAAGTTTAGGATTGTGGTGTTGATGATGGCACTATGGGCGGCTATAAAGAGAGCTATAGAGAAGGTAATGGAGTGGGAATGGTTAAGCTGGTGGCCTTTCTCTCGCCAGGAGAAGCGGCTCGAGAAACTTATTGCTGAGGCTGATGCTAATCCAAAGGACGCAGCTTTGCAGGGTGCTTTATTGGCTGAGCTCAATAAGCATATTCCTGAGGCTGTAGTTCAACGGTTTGAACAAAGGGAACACACAGTGGATAGTAGAGGAGTTGCTGAATATATTCGAGCTCTTGTCATTACAAATGCTATCTCGGAGTATCTTCCTGATGAACAAACGGGGAAGCCATCTAGTCTTCCCGCACTGCTGCAAGAGCTGAAGCATCGTGCATCAGGCAATATGGACGAATCATTTGTGAACCCTGGTATATCTGAGAAGCAACCACTGCATGTTACCATGGTTAATCCTAAAGTTTCAAACAAATCAAGATTTGCGCAAGAGCTTGTCTCTACGATCTTGTTCACTGTTGCGGTGGGATTAGTGTGGATTATGGGTGCAGCTGCTCTGCAAAAGTATATTGGAAGTCTTGGAGGAATTGGAACTTCGGGTGTTGGTTCAAGCTCTTCATATTCCCCAAAAGAGCTGAACAAAGAAATAACGCCAGAAAAGAATGTCAAAACATTTAAGGACGTAAAGGGTTGTGATGATGCAAAACAAGAGCTCGAAGAGGTAGTTGAATATCTCAAAAATCCATCAAAGTTTACCCGCCTTGGAGGAAAGTTGCCAAAGGGAATTCTTCTAACCGGGGCACCTGGTACTGGAAAGACATTGCTGGCCAAAGCAATTGCTGGAGAAGCTGGAGTACCCTTCTTCTATAGAGCTGGATCTGAATTTGAAGAGATGTTTGTTGGGGTAGGAGCTCGCCGTGTGAGATCCTTGTTTCAGGCAGCCAAGAAGAAGGCACCTTGTATTATTTTCATTGACGAAATTGATGCTGTTGGATCCACAAGAAAACAATGGGAAGGGCATACAAAGAAGACATTGCATCAACTACTTGTTGAAATGGATGGTTTTGAGCAGAATGAGGGAATAATAGTTATGGCTGCAACAAACTTGCCTGATATACTTGATCCAGCTCTGACAAGACCGGGTAGATTTGATAGACATATTGTCGTACCCAGTCCGGATGTCCGTGGACGCGAAGAGATTTTGGAACTCTACCTCCAAGGCAAACCTATGTCAGAAGATGTTGATGTTAAAGCAATTGCTCGTGGAACTCCTGGATTCAATGGTGCTGATCTTGCAAACCTGGTAAATATTGCTGCCATAAAAGCAGCTGTTGAAGGTGCTGAGAAGTTATCTTCTGAACAGTTGGAATTCGCAAAGGACCGAATTGTCATGGGCACAGAGAGGAAGACAATGTTCGTATCAGAGGACTCAAAAAAGCTCACTGCATACCATGAGAGTGGTCATGCTATTGTCGCTTTAAATACGAAAGGTGCGCACCCGATCCACAAAGCGACTATAATGCCACGTGGATCTGCATTGGGAATGGTTACACAGTTACCTTCAAACGATGAAACATCAGTGAGCAAGAGGCAGTTATTAGCTCGTCTCGATGTATGTATGGGAGGAAGAGTTGCAGAGGAGCTCATCTTTGGCTTGGACCATATTACCACTGGAGCGAGTAGCGATCTGTCACAGGCCACTGAACTTGCACAATACATGGTATCGAGTTGTGGGATGAGTGAGGCAATAGGACCAGTTCATATAAAAGAAAGACCAAGCTCTGATATGCAATCACGCATTGATGCCGAGGTTGTGAAGCTTCTTAGAGAAGCATATGAGAGAGTGAAATCTCTCTTGAAACGGCATGAGAAGCAGCTACACACGTTAGCAAACGCACTTCTAGAGTATGAAACGCTAACCGCAGAGGACATAAAGCGTATTCTCCTTCCAAAGCAGGAAGGCGAGAAATTTGAAGAGCAACAACAAGAAGAAGGAGACTTGGTATTGGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004176 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006508 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009628 GO:0009941 GO:0009987 GO:0010304 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019538 GO:0019866 GO:0030163 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042623 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044422 GO:0044424 GO:0044429 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0055035 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04139 map04139

Pfam Domains

Protein Families

AAA Peptidase_M41

Protein Analysis

806

Amino Acids

88.72

Weight (kDa)

5.8

Isoelectric Point (pI)

46.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	398 - 526	3e-42	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	549 - 592	8.3e-10	AAA+ lid domain
Peptidase_M41	PF01434	608 - 783	8.1e-61	Peptidase family M41

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012486)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53170
fragaria_vesca	FvH4_5g23210 FvH4_5g23210 FvH4_5g23210
malus_domestica	MD06G1038500.v1.1
prunus_persica	Prupe.5G026500_v2.0.a1
pyrus_communis	pycom06g03220
rosa_chinensis	RchiOBHm_Chr7g0212141
rosa_laevigata	RLG00000002923
rosa_multiflora	Rmu_sc0002283.1_g000047 Rmu_sc0013167.1_g000005
rosa_roxburghii	Rroxscaffold_3G00247080
rosa_rugosa	Rorug07G0131400 Rorug07G0131500
rosa_samantha	Rh7AG264400 Rh7BG258300 Rh7CG280900 Rh7DG270800
rosa_wichuraiana	Rw7G022570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1793
Acc16I	TGCGCA	2 cut(s)	894, 1902
AccB1I	GGYRCC	2 cut(s)	1207, 1360
AccII	CGCG	1 cut(s)	1599
AccIII	TCCGGA	1 cut(s)	1582
AciI	CCGC	8 cut(s)	234, 356, 446, 523, 759, 929, 1165, 2328
AclWI	GGATC	7 cut(s)	1291, 1326, 1395, 1408, 1523, 1903, 1945
AcoI	YGGCCR	1 cut(s)	1233
AcsI	RAATTY	4 cut(s)	1191, 1289, 1775, 2375
AcuI	CTGAAG	2 cut(s)	126, 797
AcvI	CACGTG	1 cut(s)	1934
AdeI	CACNNNGTG	1 cut(s)	655
AfaI	GTAC	3 cut(s)	1216, 1263, 1575
AfiI	CCNNNNNNNGG	1 cut(s)	1217
AflIII	ACRYGT	1 cut(s)	2289
AhdI	GACNNNNNGTC	1 cut(s)	1568
AjnI	CCWGG	5 cut(s)	513, 820, 1210, 1678, 1707
AloI	GAACNNNNNNTCC	2 cut(s)	1605, 1637
Alw21I	GWGCWC	5 cut(s)	600, 691, 1122, 1321, 2052
Alw26I	GTCTC	5 cut(s)	296, 326, 910, 2018, 2397
AlwI	GGATC	7 cut(s)	1291, 1326, 1395, 1408, 1523, 1903, 1945
Ama87I	CYCGRG	1 cut(s)	527
Aor13HI	TCCGGA	1 cut(s)	1582
AoxI	GGCC	4 cut(s)	500, 1233, 2103, 2415
ApeKI	GCWGC	9 cut(s)	569, 766, 956, 959, 1346, 1502, 1721, 1733, 2281
ApoI	RAATTY	4 cut(s)	1191, 1289, 1775, 2375
ArsI	GACNNNNNNTTYG	4 cut(s)	221, 253, 794, 826
Asp700I	GAANNNNTTC	1 cut(s)	681
AspLEI	GCGC	2 cut(s)	895, 1903
AspS9I	GGNCC	3 cut(s)	1786, 2065, 2159
AsuC2I	CCSGG	2 cut(s)	1204, 1548
AsuHPI	GGTGA	1 cut(s)	338
AvaI	CYCGRG	1 cut(s)	527
AvaII	GGWCC	3 cut(s)	1786, 2065, 2159
AxyI	CCTNAGG	1 cut(s)	615
BaeGI	GKGCMC	2 cut(s)	1210, 1805
BaeI	ACNNNNGTAYC	2 cut(s)	273, 306
BalI	TGGCCA	1 cut(s)	1235
BamHI	GGATCC	1 cut(s)	1400
BanI	GGYRCC	2 cut(s)	1207, 1360
BanII	GRGCYC	5 cut(s)	600, 691, 1122, 1321, 2052
BarI	GAAGNNNNNNTAC	2 cut(s)	1245, 1277
BauI	CACGAG	1 cut(s)	1668
BbrPI	CACGTG	1 cut(s)	1934
BbsI	GAAGAC	3 cut(s)	746, 1442, 1820
Bbv12I	GWGCWC	5 cut(s)	600, 691, 1122, 1321, 2052
BbvCI	CCTCAGC	1 cut(s)	543
BbvI	GCAGC	9 cut(s)	581, 753, 946, 968, 1358, 1489, 1708, 1745, 2293
BccI	CCATC	4 cut(s)	427, 754, 1159, 1460
BceAI	ACGGC	2 cut(s)	1308, 2285
BcgI	CGANNNNNNTGC	4 cut(s)	1373, 1407, 1909, 1943
BciT130I	CCWGG	5 cut(s)	515, 822, 1212, 1680, 1709
BcnI	CCSGG	2 cut(s)	1204, 1548
BcoDI	GTCTC	5 cut(s)	296, 326, 910, 2018, 2397
BfaI	CTAG	2 cut(s)	750, 2309
BfmI	CTRYAG	3 cut(s)	462, 621, 1273
BlpI	GCTNAGC	1 cut(s)	594
Bme1390I	CCNGG	7 cut(s)	515, 822, 1204, 1212, 1548, 1680, 1709
Bme18I	GGWCC	3 cut(s)	1786, 2065, 2159
BmeRI	GACNNNNNGTC	1 cut(s)	1568
BmeT110I	CYCGRG	1 cut(s)	527
BmgT120I	GGNCC	3 cut(s)	1786, 2065, 2159
BmiI	GGNNCC	3 cut(s)	1209, 1362, 1402
BmrFI	CCNGG	7 cut(s)	515, 822, 1204, 1212, 1548, 1680, 1709
BmrI	ACTGGG	1 cut(s)	1572
BmsI	GCATC	7 cut(s)	541, 790, 797, 1096, 1381, 1453, 2200
BmuI	ACTGGG	1 cut(s)	1572
BpiI	GAAGAC	3 cut(s)	746, 1442, 1820
BplI	GAGNNNNNCTC	2 cut(s)	890, 922
BpmI	CTGGAG	3 cut(s)	1269, 1278, 2100
Bpu10I	CCTNAGC	1 cut(s)	543
Bpu1102I	GCTNAGC	1 cut(s)	594
BpuEI	CTTGAG	1 cut(s)	106
BpuMI	CCSGG	2 cut(s)	1204, 1548
BsaAI	YACGTR	1 cut(s)	1934
BsaBI	GATNNNNATC	1 cut(s)	555
BsaJI	CCNNGG	7 cut(s)	820, 855, 1168, 1203, 1591, 1624, 2214
BsaWI	WCCGGW	1 cut(s)	1582
BsaXI	ACNNNNNCTCC	5 cut(s)	1605, 1635, 2022, 2052, 2394
Bsc4I	CCNNNNNNNGG	1 cut(s)	1217
Bse1I	ACTGG	4 cut(s)	1222, 1578, 2083, 2162
Bse21I	CCTNAGG	1 cut(s)	615
Bse3DI	GCAATG	3 cut(s)	182, 1226, 1439
Bse8I	GATNNNNATC	1 cut(s)	555
BseAI	TCCGGA	1 cut(s)	1582
BseBI	CCWGG	5 cut(s)	515, 822, 1212, 1680, 1709
BseDI	CCNNGG	7 cut(s)	820, 855, 1168, 1203, 1591, 1624, 2214
BseGI	GGATG	3 cut(s)	1471, 1591, 2148
BseJI	GATNNNNATC	1 cut(s)	555
BseLI	CCNNNNNNNGG	1 cut(s)	1217
BseMI	GCAATG	3 cut(s)	182, 1226, 1439
BseMII	CTCAG	6 cut(s)	249, 534, 585, 606, 822, 1740
BseNI	ACTGG	4 cut(s)	1222, 1578, 2083, 2162
BseRI	GAGGAG	5 cut(s)	33, 344, 360, 681, 2060
BseSI	GKGCMC	2 cut(s)	1210, 1805
BseXI	GCAGC	9 cut(s)	581, 753, 946, 968, 1358, 1489, 1708, 1745, 2293
BsgI	GTGCAG	1 cut(s)	975
Bsh1236I	CGCG	1 cut(s)	1599
BshFI	GGCC	4 cut(s)	502, 1235, 2105, 2417
BshNI	GGYRCC	2 cut(s)	1207, 1360
BsiHKAI	GWGCWC	5 cut(s)	600, 691, 1122, 1321, 2052
BsiHKCI	CYCGRG	1 cut(s)	527
BsiSI	CCGG	3 cut(s)	1203, 1547, 1583
BslFI	GGGAC	1 cut(s)	153
BslI	CCNNNNNNNGG	1 cut(s)	1217
BsmAI	GTCTC	5 cut(s)	296, 326, 910, 2018, 2397
BsmBI	CGTCTC	3 cut(s)	296, 326, 2018
BsmFI	GGGAC	1 cut(s)	153
BsnI	GGCC	4 cut(s)	502, 1235, 2105, 2417
BsoBI	CYCGRG	1 cut(s)	527
Bsp1286I	GDGCHC	7 cut(s)	600, 691, 1122, 1210, 1321, 1805, 2052
Bsp13I	TCCGGA	1 cut(s)	1582
Bsp1720I	GCTNAGC	1 cut(s)	594
Bsp19I	CCATGG	1 cut(s)	855
BspACI	CCGC	8 cut(s)	234, 356, 446, 523, 759, 929, 1165, 2328
BspANI	GGCC	4 cut(s)	502, 1235, 2105, 2417
BspCNI	CTCAG	6 cut(s)	250, 535, 586, 607, 823, 1741
BspEI	TCCGGA	1 cut(s)	1582
BspFNI	CGCG	1 cut(s)	1599
BspLI	GGNNCC	3 cut(s)	1209, 1362, 1402
BspPI	GGATC	7 cut(s)	1291, 1326, 1395, 1408, 1523, 1903, 1945
BspQI	GCTCTTC	3 cut(s)	398, 1027, 2376
BspT107I	GGYRCC	2 cut(s)	1207, 1360
BsrDI	GCAATG	3 cut(s)	182, 1226, 1439
BsrI	ACTGG	4 cut(s)	1222, 1578, 2083, 2162
BssECI	CCNNGG	7 cut(s)	820, 855, 1168, 1203, 1591, 1624, 2214
BssSI	CACGAG	1 cut(s)	1668
BssT1I	CCWWGG	3 cut(s)	855, 1168, 1624
Bst2BI	CACGAG	1 cut(s)	1668
Bst2UI	CCWGG	5 cut(s)	515, 822, 1212, 1680, 1709
Bst4CI	ACNGT	6 cut(s)	134, 633, 655, 925, 1770, 1962
Bst6I	CTCTTC	9 cut(s)	21, 91, 398, 1027, 1119, 1290, 1596, 2029, 2376
BstAPI	GCANNNNNTGC	1 cut(s)	844
BstBAI	YACGTR	1 cut(s)	1934
BstC8I	GCNNGC	2 cut(s)	1233, 1321
BstDEI	CTNAG	7 cut(s)	258, 543, 594, 615, 831, 1749, 2231
BstDSI	CCRYGG	2 cut(s)	855, 1591
BstF5I	GGATG	3 cut(s)	1471, 1591, 2148
BstFNI	CGCG	1 cut(s)	1599
BstHHI	GCGC	2 cut(s)	895, 1903
BstMAI	GTCTC	5 cut(s)	296, 326, 910, 2018, 2397
BstNI	CCWGG	5 cut(s)	515, 822, 1212, 1680, 1709
BstNSI	RCATGY	1 cut(s)	851
BstSCI	CCNGG	7 cut(s)	513, 820, 1202, 1210, 1546, 1678, 1707
BstSFI	CTRYAG	3 cut(s)	462, 621, 1273
BstSLI	GKGCMC	2 cut(s)	1210, 1805
BstUI	CGCG	1 cut(s)	1599
BstV1I	GCAGC	9 cut(s)	581, 753, 946, 968, 1358, 1489, 1708, 1745, 2293
BstV2I	GAAGAC	3 cut(s)	746, 1442, 1820
BstX2I	RGATCY	4 cut(s)	1283, 1331, 1400, 1937
BstXI	CCANNNNNNTGG	1 cut(s)	1865
BstYI	RGATCY	4 cut(s)	1283, 1331, 1400, 1937
Bsu36I	CCTNAGG	1 cut(s)	615
BsuRI	GGCC	4 cut(s)	502, 1235, 2105, 2417
BtgI	CCRYGG	2 cut(s)	855, 1591
BtsCI	GGATG	3 cut(s)	1471, 1591, 2148
BtsI	GCAGTG	3 cut(s)	761, 842, 1848
BtsIMutI	CAGTG	8 cut(s)	660, 761, 842, 921, 1848, 1992, 2076, 2106
Cac8I	GCNNGC	2 cut(s)	1233, 1321
CfoI	GCGC	2 cut(s)	895, 1903
Cfr13I	GGNCC	3 cut(s)	1786, 2065, 2159
CseI	GACGC	3 cut(s)	176, 575, 1605
CsiI	ACCWGGT	2 cut(s)	1210, 1707
Csp6I	GTAC	3 cut(s)	1215, 1262, 1574
CspCI	CAANNNNNGTGG	2 cut(s)	2095, 2130
CviAII	CATG	7 cut(s)	848, 856, 1798, 1859, 1871, 2125, 2273
CviQI	GTAC	3 cut(s)	1215, 1262, 1574
DdeI	CTNAG	7 cut(s)	258, 543, 594, 615, 831, 1749, 2231
DraI	TTTAAA	1 cut(s)	1887
DraIII	CACNNNGTG	1 cut(s)	655
DrdI	GACNNNNNNGTC	1 cut(s)	1793
DriI	GACNNNNNGTC	1 cut(s)	1568
DseDI	GACNNNNNNGTC	1 cut(s)	1793
EaeI	YGGCCR	1 cut(s)	1233
Eam1104I	CTCTTC	9 cut(s)	21, 91, 398, 1027, 1119, 1290, 1596, 2029, 2376
Eam1105I	GACNNNNNGTC	1 cut(s)	1568
EarI	CTCTTC	9 cut(s)	21, 91, 398, 1027, 1119, 1290, 1596, 2029, 2376
Ecl136II	GAGCTC	5 cut(s)	598, 689, 1120, 1319, 2050
Eco130I	CCWWGG	3 cut(s)	855, 1168, 1624
Eco24I	GRGCYC	5 cut(s)	600, 691, 1122, 1321, 2052
Eco47I	GGWCC	3 cut(s)	1786, 2065, 2159
Eco53kI	GAGCTC	5 cut(s)	598, 689, 1120, 1319, 2050
Eco57I	CTGAAG	2 cut(s)	126, 797
Eco72I	CACGTG	1 cut(s)	1934
Eco81I	CCTNAGG	1 cut(s)	615
Eco88I	CYCGRG	1 cut(s)	527
EcoICRI	GAGCTC	5 cut(s)	598, 689, 1120, 1319, 2050
EcoRI	GAATTC	2 cut(s)	1191, 1775
EcoRII	CCWGG	5 cut(s)	513, 820, 1210, 1678, 1707
EcoT14I	CCWWGG	3 cut(s)	855, 1168, 1624
EcoT38I	GRGCYC	5 cut(s)	600, 691, 1122, 1321, 2052
ErhI	CCWWGG	3 cut(s)	855, 1168, 1624
Esp3I	CGTCTC	3 cut(s)	296, 326, 2018
FaeI	CATG	7 cut(s)	851, 859, 1801, 1862, 1874, 2128, 2276
FalI	AAGNNNNNCTT	2 cut(s)	12, 44
FaqI	GGGAC	1 cut(s)	153
FatI	CATG	7 cut(s)	847, 855, 1797, 1858, 1870, 2124, 2272
FauI	CCCGC	2 cut(s)	766, 1172
FauNDI	CATATG	1 cut(s)	2242
FokI	GGATG	3 cut(s)	1478, 1598, 2155
FriOI	GRGCYC	5 cut(s)	600, 691, 1122, 1321, 2052
FspAI	RTGCGCAY	1 cut(s)	1902
FspBI	CTAG	2 cut(s)	750, 2309
FspI	TGCGCA	2 cut(s)	894, 1902
GlaI	GCGC	2 cut(s)	894, 1902
GsuI	CTGGAG	3 cut(s)	1269, 1278, 2100
HaeIII	GGCC	4 cut(s)	502, 1235, 2105, 2417
HapII	CCGG	3 cut(s)	1203, 1547, 1583
HgaI	GACGC	3 cut(s)	176, 575, 1605
HhaI	GCGC	2 cut(s)	895, 1903
Hin1II	CATG	7 cut(s)	851, 859, 1801, 1862, 1874, 2128, 2276
Hin6I	GCGC	2 cut(s)	893, 1901
HinP1I	GCGC	2 cut(s)	893, 1901
HindIII	AAGCTT	3 cut(s)	21, 65, 2225
HinfI	GANTC	6 cut(s)	209, 230, 251, 806, 1683, 1835
HpaII	CCGG	3 cut(s)	1203, 1547, 1583
HphI	GGTGA	1 cut(s)	338
Hpy166II	GTNNAC	5 cut(s)	110, 817, 921, 1161, 1595
Hpy8I	GTNNAC	5 cut(s)	110, 817, 921, 1161, 1595
HpyCH4III	ACNGT	6 cut(s)	134, 633, 655, 925, 1770, 1962
HpyCH4IV	ACGT	3 cut(s)	1089, 1933, 2291
HpyF3I	CTNAG	7 cut(s)	258, 543, 594, 615, 831, 1749, 2231
HpySE526I	ACGT	3 cut(s)	1089, 1933, 2291
Hsp92II	CATG	7 cut(s)	851, 859, 1801, 1862, 1874, 2128, 2276
HspAI	GCGC	2 cut(s)	893, 1901
Kpn2I	TCCGGA	1 cut(s)	1582
LguI	GCTCTTC	3 cut(s)	398, 1027, 2376
LmnI	GCTCC	3 cut(s)	1316, 2047, 2081
Lsp1109I	GCAGC	9 cut(s)	581, 753, 946, 968, 1358, 1489, 1708, 1745, 2293
LweI	GCATC	7 cut(s)	541, 790, 797, 1096, 1381, 1453, 2200
MabI	ACCWGGT	2 cut(s)	1210, 1707
MaeI	CTAG	2 cut(s)	750, 2309
MaeII	ACGT	3 cut(s)	1089, 1933, 2291
MaeIII	GTNAC	6 cut(s)	244, 373, 850, 1954, 1962, 2097
MfeI	CAATTG	2 cut(s)	1242, 1662
MflI	RGATCY	4 cut(s)	1283, 1331, 1400, 1937
MhlI	GDGCHC	7 cut(s)	600, 691, 1122, 1210, 1321, 1805, 2052
MlsI	TGGCCA	1 cut(s)	1235
MluNI	TGGCCA	1 cut(s)	1235
MlyI	GAGTC	2 cut(s)	224, 1829
MmeI	TCCRAC	2 cut(s)	1378, 1752
Mox20I	TGGCCA	1 cut(s)	1235
MroI	TCCGGA	1 cut(s)	1582
MroXI	GAANNNNTTC	1 cut(s)	681
MscI	TGGCCA	1 cut(s)	1235
MseI	TTAA	6 cut(s)	63, 491, 861, 1083, 1656, 1886
MslI	CAYNNNNRTG	1 cut(s)	1863
Msp20I	TGGCCA	1 cut(s)	1235
MspA1I	CMGCKG	3 cut(s)	257, 959, 1736
MspI	CCGG	3 cut(s)	1203, 1547, 1583
MspR9I	CCNGG	7 cut(s)	515, 822, 1204, 1212, 1548, 1680, 1709
MunI	CAATTG	2 cut(s)	1242, 1662
MvaI	CCWGG	5 cut(s)	515, 822, 1212, 1680, 1709
MvnI	CGCG	1 cut(s)	1599
NciI	CCSGG	2 cut(s)	1204, 1548
NcoI	CCATGG	1 cut(s)	855
NdeI	CATATG	1 cut(s)	2242
NlaIII	CATG	7 cut(s)	851, 859, 1801, 1862, 1874, 2128, 2276
NlaIV	GGNNCC	3 cut(s)	1209, 1362, 1402
NmeAIII	GCCGAG	1 cut(s)	2239
NmuCI	GTSAC	1 cut(s)	2097
NsbI	TGCGCA	2 cut(s)	894, 1902
NspI	RCATGY	1 cut(s)	851
PaeR7I	CTCGAG	1 cut(s)	527
PciSI	GCTCTTC	3 cut(s)	398, 1027, 2376
PdmI	GAANNNNTTC	1 cut(s)	681
PfeI	GAWTC	4 cut(s)	209, 251, 806, 1683
PflFI	GACNNNGTC	1 cut(s)	197
PfoI	TCCNGGA	1 cut(s)	1678
PleI	GAGTC	2 cut(s)	224, 1829
PmaCI	CACGTG	1 cut(s)	1934
PmlI	CACGTG	1 cut(s)	1934
PpsI	GAGTC	2 cut(s)	224, 1829
Ppu21I	YACGTR	1 cut(s)	1934
Psp124BI	GAGCTC	5 cut(s)	600, 691, 1122, 1321, 2052
Psp6I	CCWGG	5 cut(s)	513, 820, 1210, 1678, 1707
PspCI	CACGTG	1 cut(s)	1934
PspGI	CCWGG	5 cut(s)	513, 820, 1210, 1678, 1707
PspN4I	GGNNCC	3 cut(s)	1209, 1362, 1402
PspPI	GGNCC	3 cut(s)	1786, 2065, 2159
PsuI	RGATCY	4 cut(s)	1283, 1331, 1400, 1937
PsyI	GACNNNGTC	1 cut(s)	197
PvuII	CAGCTG	3 cut(s)	257, 959, 1736
RsaI	GTAC	3 cut(s)	1216, 1263, 1575
RsaNI	GTAC	3 cut(s)	1215, 1262, 1574
RseI	CAYNNNNRTG	1 cut(s)	1863
SacI	GAGCTC	5 cut(s)	600, 691, 1122, 1321, 2052
SapI	GCTCTTC	3 cut(s)	398, 1027, 2376
SaqAI	TTAA	6 cut(s)	63, 491, 861, 1083, 1656, 1886
Sau96I	GGNCC	3 cut(s)	1786, 2065, 2159
SchI	GAGTC	2 cut(s)	224, 1829
ScrFI	CCNGG	7 cut(s)	515, 822, 1204, 1212, 1548, 1680, 1709
SduI	GDGCHC	7 cut(s)	600, 691, 1122, 1210, 1321, 1805, 2052
SexAI	ACCWGGT	2 cut(s)	1210, 1707
SfaNI	GCATC	7 cut(s)	541, 790, 797, 1096, 1381, 1453, 2200
SfcI	CTRYAG	3 cut(s)	462, 621, 1273
Sfr274I	CTCGAG	1 cut(s)	527
SinI	GGWCC	3 cut(s)	1786, 2065, 2159
SlaI	CTCGAG	1 cut(s)	527
SmiMI	CAYNNNNRTG	1 cut(s)	1863
SmlI	CTYRAG	2 cut(s)	121, 527
SmoI	CTYRAG	2 cut(s)	121, 527
SsiI	CCGC	8 cut(s)	234, 356, 446, 523, 759, 929, 1165, 2328
SspI	AATATT	1 cut(s)	1717
SspMI	CTAG	2 cut(s)	750, 2309
SstI	GAGCTC	5 cut(s)	600, 691, 1122, 1321, 2052
StyD4I	CCNGG	7 cut(s)	513, 820, 1202, 1210, 1546, 1678, 1707
StyI	CCWWGG	3 cut(s)	855, 1168, 1624
TaaI	ACNGT	6 cut(s)	134, 633, 655, 925, 1770, 1962
TaiI	ACGT	3 cut(s)	1092, 1936, 2294
TaqI	TCGA	6 cut(s)	160, 528, 685, 1122, 2016, 2132
TaqII	GACCGA	1 cut(s)	1803
TauI	GCSGC	2 cut(s)	449, 526
TfiI	GAWTC	4 cut(s)	209, 251, 806, 1683
Tru1I	TTAA	6 cut(s)	63, 491, 861, 1083, 1656, 1886
Tru9I	TTAA	6 cut(s)	63, 491, 861, 1083, 1656, 1886
TscAI	CASTG	8 cut(s)	660, 768, 849, 928, 1855, 1992, 2083, 2113
TseFI	GTSAC	1 cut(s)	2097
TseI	GCWGC	9 cut(s)	569, 766, 956, 959, 1346, 1502, 1721, 1733, 2281
Tsp45I	GTSAC	1 cut(s)	2097
TspDTI	ATGAA	8 cut(s)	84, 208, 744, 1014, 1366, 1992, 2156, 2331
TspGWI	ACGGA	4 cut(s)	263, 299, 392, 1580
TspRI	CASTG	8 cut(s)	660, 768, 849, 928, 1855, 1992, 2083, 2113
Tth111I	GACNNNGTC	1 cut(s)	197
VpaK11BI	GGWCC	3 cut(s)	1786, 2065, 2159
XapI	RAATTY	4 cut(s)	1191, 1289, 1775, 2375
XbaI	TCTAGA	1 cut(s)	2308
XceI	RCATGY	1 cut(s)	851
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2075
XhoI	CTCGAG	1 cut(s)	527
XmnI	GAANNNNTTC	1 cut(s)	681
XspI	CTAG	2 cut(s)	750, 2309

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.