Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008640

Physical Location & Seq

Genomic Coordinates Forward (+)

21700187 .. 21710296

Gene Structure 10110 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G53460.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6627 bp

ATGTCGGCAGCTTCTTCTAGCTCCGTTCTTCACCTCCGAACCAACCAACAACTGTTATCACTCCGATCTCTCAAAAATTCCACCTCCGTTGCTTCTCAATTGGCCGTTACGTCTGGTGTTAGCCGTAGAAGATCGTGTACGGCGCGATGCTCTGTGAAGAAACCGGTGATTCCTGAGAGTCCTTTCCTAGGGACTCGTGTGCGGAGATCCGGATCCGAGACGCTTCAGTTCTGGAGATCAGATGGTCCTGGACGGTCTGCGAAGCTTCGAACGGTGGTTAAATCGTCATTTTCCGCTGTTCCGGAGAAGCCTCTTGGTCTTTATGATCCTTCCTATGATAAAGACTCTTGTGGTGTTGGTTTTGTAGCAGAGTTGTCCGGCGAGACCACCCGCAAAACGGTGACTGATTCGTTGGAGATGTTGATACGGATGACTCATAGAGGTGCTTGTGGTTGTGAGAGCAACACTGGTGACGGCGCTGGGATTCTTGTTGGTCTGCCTCATGATTTCTATGCAGAGGCTGCGACAGAGCTTGGCTTTGTACTTCCTTCTGCTGGAAATTATGCTGTTGGTATGTTCTTCTTGCCAACTGTTGAAAGCAGGAGAGAAGAAAGCAAGAATGTTTTCACTAAGGTTGCTGAATCACTTGGCCACTCGGTTCTTGGATGGAGATTAGTGCCTACCGATAATTCTGGACTGGGAAACTCCGCTTTGCAGACTGAACCGATTATTGCGCAAGTTTTCCTTACTCCTACAACTAAATCTAAAGCTGACTTTGAGCAACAGATGTATATCCTTAGAAGGGTTTCTATGGTTGCTATTCGAGCTGCGTTGAACCTTCAACATGGTGCGATGAAGGACTTCTACATCTGTTCGTTGTCTTCTAGGACTATCGTCTACAAAGGACAATTGAAGCCTGATCAACTAAAGGACTATTACTATGCGGATCTTGGGAGTGAAAGGTTTACGAGCTACATGGCCCTGGTTCATTCTCGGTTCTCTACCAATACATTTCCCAGCTGGGATCGTGCTCAGCCGATGCGTGTTTTGGGCCACAATGGGGAGATCAATACACTCAGGGGAAACGTGAACTGGATGAGGGCACGTGAGGGTCTGCTCAAGTGCAATGAACTTGGATTATCAAAGAAAGAGTTGAAGAAGCTTCTACCTATTGTGGACGTTAGCTCTTCTGACTCAGGTGCTTTTGATGGTGTTCTTGAGCTTCTTGTCCGAGCTGGTAGAAGTCTTCCTGAAGCTGTTATGATGATGATCCCTGAAGCATGGCAAAATGACAAAAACATTGATCCAAGCAGGAAAGAGTTTTATGAATACTTATCTGCTCTAATGGAGCCATGGGATGGACCTGCCCTCATATCATTCACTGATGGTCGCTACTTGGGTGCCACATTGGACCGTAATGGACTTCGTCCTGGTAGGTTCTACATAACCCACAGTGGAAGGGTTATCATGGCTAGTGAAGTTGGGGTAGTGGATGTACCACCTGAAGATGTGATGAGGAAAGGAAGACTTAACCCGGGTATGATGCTTCTTGTCGATTTTGAGAAGCATATTGTTGTTGATGACGATGCCTTGAAGCAGCAGTATTCACTGGCAAGGCCTTATGGTGAGTGGCTTAAAAGGCAAAAGATTGAACTTAAAGACATCATTGAGTCAGTTCCGGAGGCTGAACGGATTGCTCCTTCAATTTCTGGGGTTGTACCGGCCTCTAATGATGATGACTCAATGGAGAGCATGGGCATTCATGGTTTATTGTCTCCACTAAAAGCCTTTGGCTACACAGTTGAGGCCTTGGAGATGTTGCTGCTTCCGATGGCTAAAGATGGCTCTGAAGCACTTGGTTCTATGGGAAATGATACCCCTCTAGCTGTAATGTCAAACAGAGAAAAATTATGCTTTGAGTACTTCAAGCAAATGTTTGCTCAAGTAACAAATCCTCCAATTGATCCTATTCGTGAGAAAATTGTTACATCAATGGAGTGCATGATCGGTCCAGAAGGCGATCTAACCGAAACAACTGAAGAGCAATGTCACCGTCTCTCCCTAAAAGGTCCTCTTTTGAAAATCGAAGAGATGGAAGCCATCAAGAAAATGAACTACAGAGGCTGGCGGACTAAAGTTCTGGATATAACTTATGCAAAAGAAAGAGGTACAAAAGGGTTAGAGGAGACTTTGGACCGAATTTGTGATGAAGCAAATGAAGCAATAAAGGAGGGCTACACATTACTGGTTTTGTCTGATCGAGCTTTCTCTGCAACACGTGTTGCTGTGAGCTCCCTCATGGCTGTTGGTGCCGTTCATCACCACCTGGTTAAGACACTTGCGCGCACCCAAGTTGGTTTGGTAGTGGAATCTGCTGAACCACGTGAAGTGCATCATTTCTGCACACTTGTCGGATTTGGAGCAGATGCTATCTGCCCATATTTGGCTGTAGAGGCCGTTTATAGATTACAGGTTGATGGCAAAATACCACCCAAATCTAATGGGGAATTCCATTCCAAAGAAGAGTTGGTCAAGAAGTATTACAAGGCAAGTAACTATGGAATGATGAAGGTTCTTGCCAAAATGGGAATTTCTACTCTAGCCTCATACAAAGGTGCTCAGATATTTGAAGCTCTTGGGCTTTCATCTGAAGTAATTCAGAAATGTTTTGCCGGTACCCCAAGCCGAGTGGAAGGAGCAACATTTGAGATGCTTGCACGAGATGGACTTCAATTGCATGAGTTGGCCTTTCCAACCCGTGGCTATGCCCCAGGAAGTGCAGAAGCTTCAGCACTTACCAACCCTGGAAACTATCACTGGAGGAAAAATGGAGAGATTCATCTCAACGATCCTCTTGCAATAGCCAAATTACAAGAGGCAGCACGGACCAACAGTGTGGCCGCCTACAAGGAATACTCAAAGCGTATCAATGAATTGAACAAGCAAAGTAATTTGCGTGGGCTCATGAAGTTTAAAGACGCAGATGTGAAAATTCCACTAGATGAAGTCGAGCCTGCCAGTGAAATTGTTAAGCGGTTCTGCACTGGTGCCATGAGTTATGGATCAATTTCTCTGGAGGCGCATACAACCCTTGCAATGGCCATGAACAAGCTTGGAGGAAAGTCGAATACAGGTGAGGGAGGGGAACTGCCATCCCGTATGGAGCCTCTTGCGGATGGTTCAAGGAACCCCAAAAGAAGTTCGATCAAACAAATTGCTAGTGGAAGATTTGGAGTTTCTAGCTACTATCTTACAAATGCTGATGAGCTGCAGATAAAGATGGCTCAGGGTGCCAAACCTGGGGAAGGTGGGGAGCTACCAGGCCACAAGGTCATAGGAGACATTGCTATAACCAGAAATTCCACTGCTGGTGTAGGACTTATCAGTCCACCTCCTCATCATGATATCTACTCTATCGAGGATCTTGCCCAGCTAATTCACGATCTTAAGAATGCCAATCCTGGCGCACGTATTAGTGTTAAGCTTGTATCTGAAGCTGGTGTTGGAGTTATTGCTAGCGGTGTGGTGAAGGGGCATGCTGACCATGTTCTGATAGCAGGTCATGATGGAGGTACTGGTGCATCTCGCTGGACCGGTATTAAAAATGCTGGTCTTCCATGGGAACTGGGTCTAGCTGAGACACACCAAACTCTTGTTGCCAATGACCTTCGTGGCCGTACTGTTCTTCAGACAGATGGCCAGCTAAAAACTGGCAGAGACGTTGCAGTTGCTGCACTTCTTGGTGCAGAAGAATTTGGTTTTAGTACTGCACCCCTTATCACACTAGGGTGTATCATGATGAGAAAGTGCCACAAGAACACCTGCCCTGTGGGAATTGCTACCCAAGATCCAGTTCTCCGTGAAAAATTTGCTGGGGAGCCTGAACATGTTATTAACTTCTTCTTTATGCTTGCTGAGGAAGTTAGGGAGATCATGTCTGGACTTGGTTTTAGAACTGTAACTGAGATGATTGGTCGTGCGGATATGCTTGAACTTGATAGGGAAGTTGTCAAGAACAACGATAAGCTGGAGAACATTGACCTATCTCTTTTGCTCAGACCTGCTGCTGAGATTCGTCCTGGGGCTGCTCAGTACTGTGTTCAGAAACAAGATCATGGTTTAGACATGGCTTTGGATCAAGAGCTGATTGCATTGTCTAAATCTGCTCTGGAGAAATCTCTTCCGGTGTACATTGAAACTCCTATCTGCAACGTTAACCGTGCTGTTGGGACAATGCTGAGCCATGAGGTAACCAAACGTTACCACTTGACTGGGCTTCCAAAGGACACAATTCATATCAAGTTCACAGGAAGTGCTGGGCAGAGTCTTGGAGCTTTCTTGTGTCCTGGAATTATGCTAGAACTTGAAGGAGATAGCAATGACTATGTTGGAAAAGGGCTCTCTGGAGGTAAAGTTGTTGTTTACCCTCCAAAAGGAAGCAGCTTTGATCCAAAAGAAAATATCGTTATTGGTAATGTAGCACTTTACGGTGCCACTAGTGGTGAAGCATACTTCAATGGGATGGCTGCTGAAAGGTTCTCTGTTCGTAATTCTGGTGCTAAAGCTGTTGTGGAAGGTCTTGGTGATCATGGTTGTGAGTACATGACTGGTGGTACTGTTGTGGTGCTTGGAAAGACTGGTAGAAACTTTGCTGCTGGTATGAGTGGTGGTATTGCTTATGTCCTTGATGTGGACGGAAAGTTTAACACCAGATGCAACCTTGAACTGGTTGATTTGGATAAAGTTGAAGATGAGGAGGACAAAATGACGCTCAAAATGATGATCCAGCAACATCAAAGACACACCAACAGCCAACTTGCTCAAGAAGTTCTTGCAGACTTTGAGAATTTGCTGCCCAAGTTTATCAAGGTTTTCCCAAGAGATTACAAACGTGTTTTATCAGCCATGAAACACGAAGAGGTCTCCAAGCAAGCAATCGAGCGGGCTTCTGAGGAAGCTGACGAGACTGAAGAGAAAGAACTCGAGGAGAAAGATGCATTTGCAGAACTGAAGAACATGGCAGCTGCTTCGTCAAAAGAGGAGATGTCAGGAAACGGAGTGGCAGCTGAAGCTAGACCTTCTAAGGTAGATAATGCTGTTAAAAACGGTGGTTTCATTGCTTATGAGCGTGAGGGAGTTAAGTACAGGGATCCCAATGTTCGTCTTAATGACTGGAACGAAGTCATGGAGGAATCAAAACCTGGACCACTCCTTACAACTCAGTCAGCTCGTTGCATGGATTGTGGAACTCCATTCTGCCACCAGGAGAACTCTGGGTGTCCTCTCGGTAATAAGATCCCTGAATTCAATGAACTTGTCTACCAGAACAGATGGCAAGAAGCCTTGAATCGTCTACTTGAGACAAACAACTTTCCAGAATTTACTGGGCGAGTATGCCCTGCACCATGTGAAGGTTCTTGTGTTCTTGGAATAATTGAGAACCCTGTTTCTATCAAAAGCATTGAATGTGCTATTATCGATAAAGCCTTTGAGGAAGGGTGGATGGTACCAAGGCCTCCTCTCAAGAGAACAGGGAAAAAGGTTGCTATTATCGGAAGTGGACCGGCTGGGTTAGCTGCGGCTGACCAGCTGAACAAAATGGGCCATTTAGTAACCGTGTATGAGCGCTCGGACAGAATTGGTGGGCTGATGATGTATGGAGTACCAAACATGAAGACTGACAAAATTGACGTCGTTCAACGGCGGGTTGATCTTATGACCAAAGAAGGTATCAACTTTGTGGTCAATGCCAATATTGGAAAGGACCCATCTTACTCCCTTGATGGACTTAAGGAAGAGAATGATGCAATCGTTCTTGCTGTTGGTTCCACAAAACCAAGAGATCTTCCAGTGCCTGGTCGTGATCTATCTGGTGTTCACTTTGCCATGGAGTTTCTTCATGCAAACACCAAAAGTTTACTTGACAGCAATCATGAGGATGGCAATTACATTTCTGCAAAAGGGAAAAAGGTTGTTGTTATTGGTGGAGGTGATACCGGCACAGATTGCATCGGAACATCTATCCGCCATGGATGTACCAACATTGTAAACCTAGAACTTCTTCCTCAGCCACCCTCAACAAGAGCTCCTGGAAACCCTTGGCCACAGTGGCCTCGTGTATTCCGTATTGACTACGGACATCAAGAAGCTACCACCAAATTCGGAAAAGACCCTAGGACCTATGAGGTCTTAACGAAGAGGTTCATAGGAGATGACAATGGAAACGTGAAGGGGCTTGAACTTGTGCGTGTGAGTTGGGAGAAGGATGAAACCGGGAGGTTCCAGTTCAAGGAGATCGAAGGCTCTGAGGAAATCATTGAAGCAGACCTCGTTTTCTTGGCCATGGGTTTCCTTGGACCTGAGCCAACACTTGCTGAGAAGCTAGGTCTCGAATGTGACAACAGGTCAAACTTTAAGGCCGAGTATGGCCGGTTTTCCACAACCGTGGAAGGTGTTTTCGCAGCAGGTGATTGTCGGAGAGGCCAGTCTTTGGTTGTCTGGGCTATCTCAGAAGGACGTCAAGCTGCAGATCAGGTAGACAAATTCCTCACCAAAACGGATGATGACGAAGACGCCAAGCTACAACAAGACCTTAACCAGATGAAACACAACACTATCACAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015930 GO:0016040 GO:0016053 GO:0016491 GO:0016638 GO:0016639 GO:0019676 GO:0019740 GO:0019752 GO:0032502 GO:0040007 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045181 GO:0046394 GO:0046686 GO:0048589 GO:0050896 GO:0055114 GO:0060359 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00250 ko00910 ko01100 ko01110 ko01120 ko01130 ko01230 map00250 map00910 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

Fer4_20 GATase_2 GXGXG Glu_syn_central Glu_synthase Pyr_redox_2

Protein Analysis

2208

Amino Acids

241.9

Weight (kDa)

5.97

Isoelectric Point (pI)

37.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	117 - 544	1.6e-182	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	594 - 882	1.8e-114	Glutamate synthase central domain
Glu_synthase	PF01645	949 - 1317	2e-148	Conserved region in glutamate synthase
GXGXG	PF01493	1401 - 1587	2.3e-87	GXGXG motif
Fer4_20	PF14691	1724 - 1833	1.3e-21	Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
Pyr_redox_2	PF07992	1846 - 2172	3.5e-27	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	1850 - 1885	1.4e-07	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53460 AT5G53460 AT5G53460
fragaria_vesca	FvH4_7g31770 FvH4_7g31770 FvH4_7g31770
malus_domestica	MD01G1219500.v1.1 MD07G1290500.v1.1
prunus_persica	Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1
pyrus_communis	pycom01g22930 pycom07g26320
rosa_chinensis	RchiOBHm_Chr1g0381001
rosa_laevigata	RLG00000026253
rosa_multiflora	Rmu_ssc0000032.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278870
rosa_rugosa	Rorug01G0426400
rosa_samantha	Rh1AG450100 Rh1BG406100 Rh1CG420100 Rh1DG436600
rosa_wichuraiana	Rw1G039220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	3830, 6455
AasI	GACNNNNNNGTC	1 cut(s)	3384
AatII	GACGTC	2 cut(s)	5694, 6520
Acc16I	TGCGCA	1 cut(s)	735
Acc36I	ACCTGC	5 cut(s)	1372, 3548, 3830, 4069, 6455
Acc65I	GGTACC	2 cut(s)	2675, 5506
AccB1I	GGYRCC	7 cut(s)	1400, 2309, 2675, 3047, 3290, 4490, 5506
AccB7I	CCANNNNNTGG	4 cut(s)	1358, 2759, 5855, 6490
AccBSI	CCGCTC	1 cut(s)	4942
AccI	GTMKAC	4 cut(s)	897, 5319, 5353, 6537
AccII	CGCG	2 cut(s)	145, 2344
AccIII	TCCGGA	3 cut(s)	209, 301, 1678
AclI	AACGTT	2 cut(s)	4212, 4258
AcoI	YGGCCR	9 cut(s)	102, 649, 2898, 3099, 3673, 3697, 6101, 6339, 6427
AcvI	CACGTG	3 cut(s)	1106, 2279, 2384
AcyI	GRCGYC	3 cut(s)	5691, 6517, 6573
AdeI	CACNNNGTG	1 cut(s)	5408
AfeI	AGCGCT	1 cut(s)	5627
AflII	CTTAAG	2 cut(s)	3446, 5789
AflIII	ACRYGT	4 cut(s)	2276, 2278, 3886, 4891
AgeI	ACCGGT	2 cut(s)	163, 3593
AhdI	GACNNNNNGTC	1 cut(s)	6403
AhlI	ACTAGT	1 cut(s)	4496
AjuI	GAANNNNNNNTTGG	4 cut(s)	3486, 3518, 3837, 3869
AleI	CACNNNNGTG	2 cut(s)	3787, 6443
Alw21I	GWGCWC	4 cut(s)	1033, 2294, 2620, 6088
AlwNI	CAGNNNCTG	4 cut(s)	521, 2124, 3731, 5855
Ama87I	CYCGRG	2 cut(s)	1534, 4982
Aor13HI	TCCGGA	3 cut(s)	209, 301, 1678
Aor51HI	AGCGCT	1 cut(s)	5627
ArsI	GACNNNNNNTTYG	2 cut(s)	5676, 5708
AsiGI	ACCGGT	2 cut(s)	163, 3593
Asp700I	GAANNNNTTC	6 cut(s)	623, 805, 860, 2655, 6060, 6200
Asp718I	GGTACC	2 cut(s)	2675, 5506
AspA2I	CCTAGG	2 cut(s)	187, 6173
AspLEI	GCGC	8 cut(s)	145, 479, 736, 2344, 2346, 3082, 3467, 5628
AsuC2I	CCSGG	3 cut(s)	1535, 1536, 6274
AsuII	TTCGAA	1 cut(s)	268
AsuNHI	GCTAGC	1 cut(s)	3515
AvaI	CYCGRG	2 cut(s)	1534, 4982
AvrII	CCTAGG	2 cut(s)	187, 6173
BaeGI	GKGCMC	1 cut(s)	1105
BaeI	ACNNNNGTAYC	2 cut(s)	4605, 4638
BalI	TGGCCA	5 cut(s)	651, 3101, 3699, 6103, 6341
BamHI	GGATCC	2 cut(s)	212, 5149
BanI	GGYRCC	7 cut(s)	1400, 2309, 2675, 3047, 3290, 4490, 5506
BanII	GRGCYC	4 cut(s)	2294, 2964, 4401, 6088
BarI	GAAGNNNNNNTAC	4 cut(s)	121, 153, 848, 880
BauI	CACGAG	3 cut(s)	195, 2718, 6114
BbrPI	CACGTG	3 cut(s)	1106, 2279, 2384
BbsI	GAAGAC	6 cut(s)	873, 1238, 1531, 3605, 5681, 6576
Bbv12I	GWGCWC	4 cut(s)	1033, 2294, 2620, 6088
BbvCI	CCTCAGC	2 cut(s)	3915, 6066
BceAI	ACGGC	8 cut(s)	89, 108, 156, 490, 2297, 2441, 3660, 5717
BcgI	CGANNNNNNTGC	8 cut(s)	2392, 2426, 2837, 2871, 3407, 3441, 5010, 5044
BclI	TGATCA	2 cut(s)	919, 4585
BcnI	CCSGG	3 cut(s)	1535, 1536, 6274
BcuI	ACTAGT	1 cut(s)	4496
BfmI	CTRYAG	4 cut(s)	2116, 2448, 3269, 6525
BfoI	RGCGCY	2 cut(s)	480, 5629
BfrI	CTTAAG	2 cut(s)	3446, 5789
BfuAI	ACCTGC	5 cut(s)	1372, 3548, 3830, 4069, 6455
BglI	GCCNNNNNGGC	1 cut(s)	6109
BglII	AGATCT	1 cut(s)	5842
BlnI	CCTAGG	2 cut(s)	187, 6173
BlpI	GCTNAGC	2 cut(s)	1032, 4238
BmcAI	AGTACT	3 cut(s)	1922, 3766, 4094
BmeRI	GACNNNNNGTC	1 cut(s)	6403
BmeT110I	CYCGRG	2 cut(s)	1534, 4982
BmrI	ACTGGG	4 cut(s)	707, 3635, 4281, 5394
BmtI	GCTAGC	1 cut(s)	3519
BmuI	ACTGGG	4 cut(s)	707, 3635, 4281, 5394
BoxI	GACNNNNGTC	1 cut(s)	893
BpiI	GAAGAC	6 cut(s)	873, 1238, 1531, 3605, 5681, 6576
BpmI	CTGGAG	6 cut(s)	253, 2839, 3095, 4049, 4190, 4425
Bpu10I	CCTNAGC	4 cut(s)	3285, 3915, 6066, 6360
Bpu1102I	GCTNAGC	2 cut(s)	1032, 4238
Bpu14I	TTCGAA	1 cut(s)	268
BpuEI	CTTGAG	6 cut(s)	1103, 1238, 1926, 4806, 5378, 5507
BpuMI	CCSGG	3 cut(s)	1535, 1536, 6274
Bsa29I	ATCGAT	1 cut(s)	5478
BsaAI	YACGTR	4 cut(s)	1106, 2279, 2384, 3470
BsaBI	GATNNNNATC	3 cut(s)	211, 791, 1268
BsaHI	GRCGYC	3 cut(s)	5691, 6517, 6573
BsaI	GGTCTC	3 cut(s)	377, 4927, 6392
BsaWI	WCCGGW	6 cut(s)	163, 209, 301, 1678, 3593, 4183
Bse118I	RCCGGY	7 cut(s)	163, 1720, 2672, 3593, 5563, 5996, 6429
Bse3DI	GCAATG	6 cut(s)	1132, 2051, 3102, 3342, 4384, 5115
Bse8I	GATNNNNATC	3 cut(s)	211, 791, 1268
BseAI	TCCGGA	3 cut(s)	209, 301, 1678
BseCI	ATCGAT	1 cut(s)	5478
BseJI	GATNNNNATC	3 cut(s)	211, 791, 1268
BseMI	GCAATG	6 cut(s)	1132, 2051, 3102, 3342, 4384, 5115
BsePI	GCGCGC	1 cut(s)	2342
BseRI	GAGGAG	6 cut(s)	2198, 3384, 4769, 5000, 5054, 5508
BseSI	GKGCMC	1 cut(s)	1105
BseYI	CCCAGC	7 cut(s)	479, 1016, 1020, 3429, 3872, 4316, 5567
BsgI	GTGCAG	7 cut(s)	2386, 2799, 3025, 3717, 3753, 3765, 5385
Bsh1236I	CGCG	2 cut(s)	145, 2344
BshNI	GGYRCC	7 cut(s)	1400, 2309, 2675, 3047, 3290, 4490, 5506
BshTI	ACCGGT	2 cut(s)	163, 3593
BshVI	ATCGAT	1 cut(s)	5478
BsiHKAI	GWGCWC	4 cut(s)	1033, 2294, 2620, 6088
BsiHKCI	CYCGRG	2 cut(s)	1534, 4982
BslFI	GGGAC	2 cut(s)	205, 4243
BsmBI	CGTCTC	3 cut(s)	212, 2060, 3711
BsmFI	GGGAC	2 cut(s)	205, 4243
BsmI	GAATGC	2 cut(s)	1758, 3457
Bso31I	GGTCTC	3 cut(s)	377, 4927, 6392
BsoBI	CYCGRG	2 cut(s)	1534, 4982
Bsp119I	TTCGAA	1 cut(s)	268
Bsp1286I	GDGCHC	7 cut(s)	1033, 1105, 2294, 2620, 2964, 4401, 6088
Bsp13I	TCCGGA	3 cut(s)	209, 301, 1678
Bsp1407I	TGTACA	1 cut(s)	4188
Bsp1720I	GCTNAGC	2 cut(s)	1032, 4238
Bsp19I	CCATGG	5 cut(s)	1352, 3617, 5886, 6028, 6342
BspDI	ATCGAT	1 cut(s)	5478
BspEI	TCCGGA	3 cut(s)	209, 301, 1678
BspFNI	CGCG	2 cut(s)	145, 2344
BspHI	TCATGA	6 cut(s)	502, 2964, 3400, 3562, 3795, 5932
BspMAI	CTGCAG	2 cut(s)	3273, 6529
BspMI	ACCTGC	5 cut(s)	1372, 3548, 3830, 4069, 6455
BspOI	GCTAGC	1 cut(s)	3519
BspQI	GCTCTTC	2 cut(s)	1192, 2034
BspT104I	TTCGAA	1 cut(s)	268
BspT107I	GGYRCC	7 cut(s)	1400, 2309, 2675, 3047, 3290, 4490, 5506
BspTI	CTTAAG	2 cut(s)	3446, 5789
BspTNI	GGTCTC	3 cut(s)	377, 4927, 6392
BsrBI	CCGCTC	1 cut(s)	4942
BsrDI	GCAATG	6 cut(s)	1132, 2051, 3102, 3342, 4384, 5115
BsrFI	RCCGGY	7 cut(s)	163, 1720, 2672, 3593, 5563, 5996, 6429
BsrGI	TGTACA	1 cut(s)	4188
BssAI	RCCGGY	7 cut(s)	163, 1720, 2672, 3593, 5563, 5996, 6429
BssHII	GCGCGC	1 cut(s)	2342
BssNI	GRCGYC	3 cut(s)	5691, 6517, 6573
BssSI	CACGAG	3 cut(s)	195, 2718, 6114
Bst2BI	CACGAG	3 cut(s)	195, 2718, 6114
Bst6I	CTCTTC	9 cut(s)	1192, 2034, 2082, 2517, 4185, 4911, 4965, 5790, 6191
BstACI	GRCGYC	3 cut(s)	5691, 6517, 6573
BstAFI	CTTAAG	2 cut(s)	3446, 5789
BstAPI	GCANNNNNTGC	3 cut(s)	521, 3731, 6006
BstAUI	TGTACA	1 cut(s)	4188
BstBAI	YACGTR	4 cut(s)	1106, 2279, 2384, 3470
BstBI	TTCGAA	1 cut(s)	268
BstDSI	CCRYGG	7 cut(s)	1352, 2758, 3617, 5886, 6028, 6342, 6444
BstEII	GGTNACC	1 cut(s)	4249
BstENI	CCTNNNNNAGG	3 cut(s)	186, 3303, 4431
BstFNI	CGCG	2 cut(s)	145, 2344
BstH2I	RGCGCY	2 cut(s)	480, 5629
BstHHI	GCGC	8 cut(s)	145, 479, 736, 2344, 2346, 3082, 3467, 5628
BstNSI	RCATGY	2 cut(s)	3539, 3890
BstPAI	GACNNNNGTC	1 cut(s)	893
BstPI	GGTNACC	1 cut(s)	4249
BstSFI	CTRYAG	4 cut(s)	2116, 2448, 3269, 6525
BstSLI	GKGCMC	1 cut(s)	1105
BstUI	CGCG	2 cut(s)	145, 2344
BstV2I	GAAGAC	6 cut(s)	873, 1238, 1531, 3605, 5681, 6576
BstX2I	RGATCY	8 cut(s)	206, 212, 946, 3421, 3847, 5149, 5295, 5842
BstXI	CCANNNNNNTGG	3 cut(s)	2896, 4271, 6445
BstYI	RGATCY	8 cut(s)	206, 212, 946, 3421, 3847, 5149, 5295, 5842
Bsu15I	ATCGAT	1 cut(s)	5478
BsuTUI	ATCGAT	1 cut(s)	5478
BtgI	CCRYGG	7 cut(s)	1352, 2758, 3617, 5886, 6028, 6342, 6444
BtgZI	GCGATG	2 cut(s)	160, 866
BtsI	GCAGTG	1 cut(s)	3363
BveI	ACCTGC	5 cut(s)	1372, 3548, 3830, 4069, 6455
CaiI	CAGNNNCTG	4 cut(s)	521, 2124, 3731, 5855
CciI	TCATGA	6 cut(s)	502, 2964, 3400, 3562, 3795, 5932
CfoI	GCGC	8 cut(s)	145, 479, 736, 2344, 2346, 3082, 3467, 5628
Cfr10I	RCCGGY	7 cut(s)	163, 1720, 2672, 3593, 5563, 5996, 6429
Cfr9I	CCCGGG	1 cut(s)	1534
ClaI	ATCGAT	1 cut(s)	5478
CseI	GACGC	4 cut(s)	229, 2987, 4777, 6581
CsiI	ACCWGGT	1 cut(s)	2325
CspAI	ACCGGT	2 cut(s)	163, 3593
CspCI	CAANNNNNGTGG	4 cut(s)	70, 105, 2670, 2705
DraI	TTTAAA	1 cut(s)	2974
DraIII	CACNNNGTG	1 cut(s)	5408
DrdI	GACNNNNNNGTC	1 cut(s)	3384
DriI	GACNNNNNGTC	1 cut(s)	6403
DseDI	GACNNNNNNGTC	1 cut(s)	3384
EaeI	YGGCCR	9 cut(s)	102, 649, 2898, 3099, 3673, 3697, 6101, 6339, 6427
Eam1104I	CTCTTC	9 cut(s)	1192, 2034, 2082, 2517, 4185, 4911, 4965, 5790, 6191
Eam1105I	GACNNNNNGTC	1 cut(s)	6403
EarI	CTCTTC	9 cut(s)	1192, 2034, 2082, 2517, 4185, 4911, 4965, 5790, 6191
EciI	GGCGGA	2 cut(s)	2143, 6014
Ecl136II	GAGCTC	2 cut(s)	2292, 6086
Eco147I	AGGCCT	3 cut(s)	1618, 1808, 5515
Eco24I	GRGCYC	4 cut(s)	2294, 2964, 4401, 6088
Eco31I	GGTCTC	3 cut(s)	377, 4927, 6392
Eco32I	GATATC	1 cut(s)	3406
Eco47III	AGCGCT	1 cut(s)	5627
Eco53kI	GAGCTC	2 cut(s)	2292, 6086
Eco72I	CACGTG	3 cut(s)	1106, 2279, 2384
Eco88I	CYCGRG	2 cut(s)	1534, 4982
Eco91I	GGTNACC	1 cut(s)	4249
EcoICRI	GAGCTC	2 cut(s)	2292, 6086
EcoNI	CCTNNNNNAGG	3 cut(s)	186, 3303, 4431
EcoO109I	RGGNCCY	3 cut(s)	2069, 5764, 6177
EcoO65I	GGTNACC	1 cut(s)	4249
EcoRI	GAATTC	2 cut(s)	2507, 5303
EcoRV	GATATC	1 cut(s)	3406
EcoT22I	ATGCAT	1 cut(s)	4999
EcoT38I	GRGCYC	4 cut(s)	2294, 2964, 4401, 6088
Esp3I	CGTCTC	3 cut(s)	212, 2060, 3711
FalI	AAGNNNNNCTT	8 cut(s)	729, 761, 1512, 1544, 2058, 2090, 4815, 4847
FaqI	GGGAC	2 cut(s)	205, 4243
FauI	CCCGC	3 cut(s)	398, 4935, 5697
FbaI	TGATCA	2 cut(s)	919, 4585
FblI	GTMKAC	4 cut(s)	897, 5319, 5353, 6537
FriOI	GRGCYC	4 cut(s)	2294, 2964, 4401, 6088
FspI	TGCGCA	1 cut(s)	735
GlaI	GCGC	8 cut(s)	144, 478, 735, 2343, 2345, 3081, 3466, 5627
GsaI	CCCAGC	7 cut(s)	483, 1020, 1024, 3433, 3876, 4320, 5571
GsuI	CTGGAG	6 cut(s)	253, 2839, 3095, 4049, 4190, 4425
HaeII	RGCGCY	2 cut(s)	480, 5629
HgaI	GACGC	4 cut(s)	229, 2987, 4777, 6581
HhaI	GCGC	8 cut(s)	145, 479, 736, 2344, 2346, 3082, 3467, 5628
Hin1I	GRCGYC	3 cut(s)	5691, 6517, 6573
Hin6I	GCGC	8 cut(s)	143, 477, 734, 2342, 2344, 3080, 3465, 5626
HinP1I	GCGC	8 cut(s)	143, 477, 734, 2342, 2344, 3080, 3465, 5626
HincII	GTYRAC	1 cut(s)	4216
HindII	GTYRAC	1 cut(s)	4216
HindIII	AAGCTT	5 cut(s)	263, 1160, 2784, 3110, 3482
HpaI	GTTAAC	1 cut(s)	4216
Hpy99I	CGWCG	1 cut(s)	5696
Hsp92I	GRCGYC	3 cut(s)	5691, 6517, 6573
HspAI	GCGC	8 cut(s)	143, 477, 734, 2342, 2344, 3080, 3465, 5626
Kpn2I	TCCGGA	3 cut(s)	209, 301, 1678
KpnI	GGTACC	2 cut(s)	2679, 5510
Ksp22I	TGATCA	2 cut(s)	919, 4585
KspAI	GTTAAC	1 cut(s)	4216
LguI	GCTCTTC	2 cut(s)	1192, 2034
MabI	ACCWGGT	1 cut(s)	2325
MbiI	CCGCTC	1 cut(s)	4942
MfeI	CAATTG	4 cut(s)	98, 908, 1959, 2732
MflI	RGATCY	8 cut(s)	206, 212, 946, 3421, 3847, 5149, 5295, 5842
MhlI	GDGCHC	7 cut(s)	1033, 1105, 2294, 2620, 2964, 4401, 6088
MlsI	TGGCCA	5 cut(s)	651, 3101, 3699, 6103, 6341
MluNI	TGGCCA	5 cut(s)	651, 3101, 3699, 6103, 6341
MlyI	GAGTC	8 cut(s)	187, 187, 338, 427, 1187, 1679, 1733, 4333
MmeI	TCCRAC	6 cut(s)	393, 2392, 2777, 3484, 4369, 6455
Mox20I	TGGCCA	5 cut(s)	651, 3101, 3699, 6103, 6341
Mph1103I	ATGCAT	1 cut(s)	4999
MroI	TCCGGA	3 cut(s)	209, 301, 1678
MroXI	GAANNNNTTC	6 cut(s)	623, 805, 860, 2655, 6060, 6200
MscI	TGGCCA	5 cut(s)	651, 3101, 3699, 6103, 6341
MslI	CAYNNNNRTG	5 cut(s)	441, 3787, 4593, 5937, 6443
Msp20I	TGGCCA	5 cut(s)	651, 3101, 3699, 6103, 6341
MspA1I	CMGCKG	5 cut(s)	296, 1020, 5024, 5066, 5590
MspCI	CTTAAG	2 cut(s)	3446, 5789
MunI	CAATTG	4 cut(s)	98, 908, 1959, 2732
Mva1269I	GAATGC	2 cut(s)	1758, 3457
MvnI	CGCG	2 cut(s)	145, 2344
NciI	CCSGG	3 cut(s)	1535, 1536, 6274
NcoI	CCATGG	5 cut(s)	1352, 3617, 5886, 6028, 6342
NheI	GCTAGC	1 cut(s)	3515
NmeAIII	GCCGAG	2 cut(s)	2711, 6445
NmuCI	GTSAC	4 cut(s)	400, 470, 2048, 6395
NsbI	TGCGCA	1 cut(s)	735
NsiI	ATGCAT	1 cut(s)	4999
NspI	RCATGY	2 cut(s)	3539, 3890
NspV	TTCGAA	1 cut(s)	268
OliI	CACNNNNGTG	2 cut(s)	3787, 6443
PaeI	GCATGC	1 cut(s)	3539
PaeR7I	CTCGAG	1 cut(s)	4982
PagI	TCATGA	6 cut(s)	502, 2964, 3400, 3562, 3795, 5932
PaqCI	CACCTGC	2 cut(s)	3830, 6455
PauI	GCGCGC	1 cut(s)	2342
PceI	AGGCCT	3 cut(s)	1618, 1808, 5515
PciI	ACATGT	1 cut(s)	3886
PciSI	GCTCTTC	2 cut(s)	1192, 2034
PcsI	WCGNNNNNNNCGW	1 cut(s)	6121
PctI	GAATGC	2 cut(s)	1758, 3457
PdmI	GAANNNNTTC	6 cut(s)	623, 805, 860, 2655, 6060, 6200
PfeI	GAWTC	9 cut(s)	169, 407, 484, 641, 2369, 2836, 4072, 5192, 5347
PflFI	GACNNNGTC	1 cut(s)	1425
PflMI	CCANNNNNTGG	4 cut(s)	1358, 2759, 5855, 6490
PfoI	TCCNGGA	3 cut(s)	247, 4345, 6088
PinAI	ACCGGT	2 cut(s)	163, 3593
PleI	GAGTC	8 cut(s)	186, 187, 338, 427, 1187, 1678, 1733, 4332
PmaCI	CACGTG	3 cut(s)	1106, 2279, 2384
PmlI	CACGTG	3 cut(s)	1106, 2279, 2384
PpsI	GAGTC	8 cut(s)	186, 187, 338, 427, 1187, 1678, 1733, 4332
Ppu21I	YACGTR	4 cut(s)	1106, 2279, 2384, 3470
PpuMI	RGGWCCY	3 cut(s)	2069, 5764, 6177
PscI	ACATGT	1 cut(s)	3886
PshAI	GACNNNNGTC	1 cut(s)	893
Psp124BI	GAGCTC	2 cut(s)	2294, 6088
Psp1406I	AACGTT	2 cut(s)	4212, 4258
Psp5II	RGGWCCY	3 cut(s)	2069, 5764, 6177
PspCI	CACGTG	3 cut(s)	1106, 2279, 2384
PspEI	GGTNACC	1 cut(s)	4249
PspFI	CCCAGC	7 cut(s)	479, 1016, 1020, 3429, 3872, 4316, 5567
PspPPI	RGGWCCY	3 cut(s)	2069, 5764, 6177
PspXI	VCTCGAGB	1 cut(s)	4982
PstI	CTGCAG	2 cut(s)	3273, 6529
PstNI	CAGNNNCTG	4 cut(s)	521, 2124, 3731, 5855
PsuI	RGATCY	8 cut(s)	206, 212, 946, 3421, 3847, 5149, 5295, 5842
PsyI	GACNNNGTC	1 cut(s)	1425
PteI	GCGCGC	1 cut(s)	2342
PvuII	CAGCTG	4 cut(s)	1020, 5024, 5066, 5590
RseI	CAYNNNNRTG	5 cut(s)	441, 3787, 4593, 5937, 6443
SacI	GAGCTC	2 cut(s)	2294, 6088
SapI	GCTCTTC	2 cut(s)	1192, 2034
ScaI	AGTACT	3 cut(s)	1922, 3766, 4094
SchI	GAGTC	8 cut(s)	187, 187, 338, 427, 1187, 1679, 1733, 4333
SduI	GDGCHC	7 cut(s)	1033, 1105, 2294, 2620, 2964, 4401, 6088
SexAI	ACCWGGT	1 cut(s)	2325
SfcI	CTRYAG	4 cut(s)	2116, 2448, 3269, 6525
SfiI	GGCCNNNNNGGCC	1 cut(s)	6109
Sfr274I	CTCGAG	1 cut(s)	4982
SfuI	TTCGAA	1 cut(s)	268
SlaI	CTCGAG	1 cut(s)	4982
SmaI	CCCGGG	1 cut(s)	1536
SmiMI	CAYNNNNRTG	5 cut(s)	441, 3787, 4593, 5937, 6443
SmlI	CTYRAG	9 cut(s)	1118, 1217, 1941, 3446, 4821, 4982, 5357, 5522, 5789
SmoI	CTYRAG	9 cut(s)	1118, 1217, 1941, 3446, 4821, 4982, 5357, 5522, 5789
SpeI	ACTAGT	1 cut(s)	4496
SphI	GCATGC	1 cut(s)	3539
SseBI	AGGCCT	3 cut(s)	1618, 1808, 5515
SspI	AATATT	1 cut(s)	5755
SstI	GAGCTC	2 cut(s)	2294, 6088
StuI	AGGCCT	3 cut(s)	1618, 1808, 5515
TaqII	GACCGA	2 cut(s)	1997, 2211
TatI	WGTACW	7 cut(s)	541, 1920, 3764, 4092, 4188, 4599, 5142
TauI	GCSGC	2 cut(s)	2903, 5582
TfiI	GAWTC	9 cut(s)	169, 407, 484, 641, 2369, 2836, 4072, 5192, 5347
TseFI	GTSAC	4 cut(s)	400, 470, 2048, 6395
Tsp45I	GTSAC	4 cut(s)	400, 470, 2048, 6395
TspMI	CCCGGG	1 cut(s)	1534
Tth111I	GACNNNGTC	1 cut(s)	1425
Van91I	CCANNNNNTGG	4 cut(s)	1358, 2759, 5855, 6490
Vha464I	CTTAAG	2 cut(s)	3446, 5789
XagI	CCTNNNNNAGG	3 cut(s)	186, 3303, 4431
XceI	RCATGY	2 cut(s)	3539, 3890
XcmI	CCANNNNNNNNNTGG	4 cut(s)	659, 2524, 3707, 5270
XhoI	CTCGAG	1 cut(s)	4982
XmaI	CCCGGG	1 cut(s)	1534
XmaJI	CCTAGG	2 cut(s)	187, 6173
XmiI	GTMKAC	4 cut(s)	897, 5319, 5353, 6537
XmnI	GAANNNNTTC	6 cut(s)	623, 805, 860, 2655, 6060, 6200
ZraI	GACGTC	2 cut(s)	5692, 6518
ZrmI	AGTACT	3 cut(s)	1922, 3766, 4094
Zsp2I	ATGCAT	1 cut(s)	4999

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.