Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0008640

Physical Location & Seq

Genomic Coordinates Reverse (-)

1801483 .. 1811884

Gene Structure 10402 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00278870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5715 bp

ATGCGTGTCATTGGTCACAATGGAGAAATCAACACCCTTAGAGGAAATGTTAACTGGATGAAGGCACGTGAGGGACTGCTTAAGTGTACTGAACTTGGTTTATCAAAAAATGAGTTAAAGAAGCTGTTACCAATTGTGGATGCCAGTTCATCCGATTCAGGAGCTTTTGATGGTGTCCTTGAGCTTCTAGTTCGAGCTGGCAGAAGCCTCCCTGAAGCTATTATGATGATGATTCCTGAAGCATGGCAAAATGACAAGAACATGGATCCTGATAGGAGAGCACTGTATGAATATTTCTCATCTCTCATGGAGCCATGGGATGGGCCAGCTCTAATATCATGTAATATATGTGGTCATTTTTTTGGATTTCTATGGAATACATACAGAATGCCTGCTTTGTACATTTTTGATTTACTAAAAACAATTATTGCAGTTACTGATGGACGCTATCTGGGAGCTACATTGGATCGGAATGGACTGCGACCGGGTCGCTTTTACGTCACCCACAGTGGACGAGTTATTATGGCCAGTGAAGTTGGGGTTGTAGACGTACCTCCAGAAGATGTCTGCAGGAAAGGAAGGCTTAACCCTGGAATGATGCTTTTGGTGGATTTTGAGAATCATATAGTGGTAGATGATGAAGCCTTGAAGCAGCAATATTCACTGGCAAGGCCGTATGGGGAGTGGCTTAAAAGGCAGAAGATTGAGCTCAAGGACATAGTTGATTCTGTTCATGAATCTGACAGGGTCCCTCCATCCATAGCTGGAGTTGTTCCTGCATCTACTGATGATGACAACATGGAAAACATGGGAGTCCATGGTTTACTGGCACCACTGAAGGCTTTTGGTTACACAGTTGAAGCCTTGGAAATGTTGTTGCTTCCTATGGCAAAAGATGGCGTAGAGGCTCTTGGGTCAATGGGAAATGATACCCCCTTGGCTGTTATGTCCAACAGAGAAAAGCTTACATTTGAGTATTTCAAGCAAATGTTCGCCCAAGTAACAAACCCTCCAATTGATCCAATTCGAGAAAAGGTAGTCACGTCTATGGAATGTATGATCGGTCCAGAAGGTGATCTAACAGAAACCACTGAAGAACAATGCCATCGTCTCTCACTCAAAGGTCCTCTTTTAAGCATTGAGGAAATGGAGGCGATCAAAAAAATGAATTACAGAGGTTGGTGCTGTAAGGTTCTTGACATAACTTACTCCAAGGAACGCGGTAGAAAGGGATTGGAGGAGACCTTGGATAGGATCTGTGCTGAAGCACGCGAGGCAATTAAAAAGGGATATACCACACTGGTGCTTTCAGACAGAGCCTTCTCCCCAAAACGTGTTGCTGTAAGCTCACTTTTGGCTGTTGGTGCTGTTCATCAACATCTAGTTAAAAATCTTGAGCGCACGCGAGTAGGGTTGATAATTGAATCTGCTGAGCCGCGTGAGGTACACCATTTCTGTACGTTAGTTGGATTCGGTGCAGATGCCATCTGCCCCTACCTGGCTGTAGAGGCTATTTGGAGACTGCATGTTGATGGAAAGATCCCACCAGAATCTAATGGTACAATATATTCCAAGGATGAACTGGTTAAAAAGTACTTCAAAGCAAGCAACTATGGAATGCAGAAGGTTCTTGCCAAGATGGGGATATCTACTTTGGCCTCTTACAAGGGTGCACAGATTTTTGAAGCTTTGGGTCTTTCATCTGAAGTGATTGAGAGGTGCTTTGCAGGAACTCCTAGTAGAGTTGAGGGGGCAACATTTGAGATGCTTGCTCGTGATGGACTTCATCTGCATGATTTGGCATTTCCCTCTCGGGCTTTTCCTCCAGGTAGTGCAGAAGCTGTGGCACTACCAAATCCAGGGGATTATCATTGGAGAAAAGGTGGTGAAGTTCACCTGAATGATCCTTTTGCTATTTCCAAACTTCAGGAGGCTGCCAGAACTAACAGTGTGGCGGCATATAAAGAATACTCCAAGCTCATTCATCAATTAAATAAAGCCTGCAATCTCCGTGGTCTTCTGAAATTTAAAAACACAGAGCAGCAAATTCACTTGGATGAAGTGGAACCTGCCAGTGAGATTGTTAAACGGTTCTGTACTGGGGCAATGAGTTATGGATCAATATCATTGGAGGCACACACTACACTGGCCATTGCTATGAACAGAATGGGAGGAAAGTCGAATACAGGTGAGGGAGGTGAGCAGCCCTCTCGTATGGAGCCTCTTCCTGATGGTTCAAGGAATCCAAAGAGGAGTGCAATTAAGCAGGTTGCAAGTGGGAGATTTGGAGTTTCAAGTTACTATCTTACAAATGCTGATGAACTCCAAATAAAAATGGCTCAGGGGGCCAAGCCCGGTGAAGGAGGTGAGCTTCCTGGTCACAAGGTTATTGGAGACATTGCTGTTACCAGGAATTCCACTGCTGGTGTGGGACTTATCAGCCCACCTCCCCATCATGATATTTACTCAATTGAAGACCTTGCTCAATTAATTCATGATCTTAAGAATGCTAACCCAGGGGCTCGAATCAGCGTCAAGTTAGTGTCTGAAGCTGGTGTAGGAGTAGTTGCTAGTGGAGTTGTGAAGGGGCATGCCGACCATGTTTTAATTGCCGGACATGATGGAGGCACTGGGGCCTCCAGATGGACCGGCATTAAGAATGCTGGGCTTCCATGGGAACTTGGCTTGGCTGAGACCCATCAAACCCTGGTTGCTAATGATCTTCGTGGCCGCACAGTTCTCCAGACAGATGGCCAACTAAAAACTGGAAGAGATGTGGCCATAGCCGCACTTCTTGGTGCAGAAGAGTTTGGGTTCAGCACGGCACCCCTCATAACCCTTGGCTGCATTATGATGCGGAAGTGCCACAAGAACACCTGTCCTGTCGGAATTGCAACCCAAGATCCAGTACTCCGTGAGAAGTTTGCCGGAGAACCTGAACATGTTATCAACTTTTTCTTCATGGTAGCTGAGGAGGTGAGAGAGATAATGTCACAACTTGGTTTTCGAACTTTAAATGAAATGGTTGGTCGTTCAGATATGCTTGAAGTCGACAAAGAAGTTACTATAGGCAATGAGAAGCTGGACAACATTGATCTTTCCCTGTTACTTAGACCTGCGGCAGACATTCGTCCTGAAGCTGCACAATATTGTGTTCAGAAACAGGACCATGGGTTGGACATGGCTCTTGACCACAAACTTATTTCGCTGTCCAGTTCTGCTATAGAGAAGGCTCTTCCTGCATACTTTGAAACACCAATTTGCAATGTGAATCGTGCTGTTGGAACAATGCTTAGTCATGAGGTGACCAAACGTTACAACAGGGAAGGGCTTCCAGCAGACACCATTCATATCAAATTCAACGGAAGTGCAGGCCAGAGCCTTGGAGCTTTCCTCTGCCCTGGCATCACGCTTGAGCTTGAAGGAGACAGCAATGATTACGTTGGTAAAGGATTGTCTGGTGGCAAGATCATAGTTTATCCCCCCAAGGGAAGCGAGTTTGACCCAAAAGAAAACATTGTCATTGGTAATGTAGCTCTTTATGGTGCCACTAGTGGGGAGGCATATTTCAATGGAATGGCAGCAGAAAGATTCTGTGTTCGCAATTCAGGGGCAAGGGCAGTAGTTGAAGGTGTCGGTGATCATGGCTGTGAGTACATGACAGGTGGCACTGTTGTTGTGCTTGGGAAAACTGGCAGGAACTTTGCTGCTGGTATGAGTGGTGGCATAGCCTACGTTCTTGATGTGGATGGAAAATTTTTGTCCCGATGCAATCCTGAGCTTGTAGATCTTGATAAAGTTGAAGGAGAAGATGGTATGACTCTTAAAATGATGATACAGCAGCATCAGCGTCATACAAAAAGCTTGCTGGCCAGTGAAGTACTGGCTGATTTTGAGAATCTTTTGCCTAAATTCATCAAGGTTATCCCCAGAGAGTACAAGCGTGCACTTGCAAACCTGAGGGAGGAGGCCTCCAAACAGGCAGTTGATCAAGCTGATGAAGAAGCTCCGGAGCAAGAGGAAGAGGTGGAAATAAAGGAGAAAGATGCATTTGAAGAACTTAAGAAGATGGCATCTGCATCTTTAAATGGGACATCTAATCAGGTAGAAGATGCTGAAACATTGAAGAGGCCTAGTGAGGTCAGCAAAGCTGTTAAACACCGGGGTTTCATTTCTTATGCGCGTGAGGGCGTTCAATACAGGGATCCTAAAGTTCGGATGAATGACTGGAAGGAAGTTATGGAGGAAACTAAACCTGGTCCACTTGTGAATACGCAGTCAGCGCGTTGCATGGACTGTGGCACTCCTTTCTGCCATCAGGAAAACACTGGGTGTCCTCTTGGTAACAAAATACCTGAGTTCAATGAATTGGTGTACCAGAATAGGTGGCATGAAGCATTAGAGAGACTCCTCGAGACAAATAACTTCCCTGAGTTTACTGGTCGAGTTTGCCCTGCACCTTGTGAAGGTTCATGTGTTCTGGGTATTATTGAGAATCCTGTCTCTATTAAAAGCATTGAATGTGCCATCATAGACAAGGCTTTTGAGGAAGGTTGGATGGTGCCACGGCCTCCACTCAAGAGAACTGGGAAAAAGGTTGCCATTGTCGGAAGTGGGCCTGCTGGGCTAGCTGCTGCTGATCAACTAAACAGAATAGGTCATACAGTGACAGTTTACGAGCGTGCTGATAGAATTGGAGGGCTGATGATGTATGGAGTGCCTAATATGAAGGCTGACAAAGTTGATATAGTTCAACGGCGGGTTAACCTTATGGCCGAGGAAGGTGTCAACTTTGTGGTCAATGCTAATGTTGGAAATGATCCTTCCTACTCGTTTGATCGCCTTCGGGAGGAGAACAATGCGATTATTTTAGCAGTAGGCGCAACGAAGCCAAGGGACCTTCCCGTACCTGGGCGAGAGCTGTCAGGAGTACATTTTGCTATGGAGTTTCTTCATGCAAACACCAAAAGCTTGCTTGATAGCAATCTCGAGAATGGTAACTACATTTCTGCCAAGGGTAAGAAGGTTGTGGTCATTGGTGGAGGTGACACTGGCACTGATTGTATTGGAACCTCTGTTCGCCATGGCTGTACTAATGTCGTAAATTTGGAGCTTCTCCCTGAGCCACCACGAACAAGGGCACCAGGAAACCCTTGGCCACAGTGGCCTCGTATATTCCGAGTAGATTATGGGCATGCAGAAGTTGCAGCCAAGTTTGGCAAAGATCCAAGAACTTATGAAGTGCTGACTAAGAGGTTTGTGGGAGATGAGAATGGGGTTGTGAAGGGACTTGAGGTAGTACGTGTCAAATGGGAGAAGGATGCTACTGGCAAATTCCAATTTAAGGAAATCGAGGGCTCTGAGGAGACAATTGAGGCTGATCTCGTCCTTCTAGCAATGGGGTTCCTTGGACCCGAGGCGACGATTGCAGAGAAGTTGGGTTTGGAGCGTGACAATCGATCAAACTTCAAGGCAGATTATGGTCGGTTCTCAACCAATGTGGATGGGGTCTTCGCTGCTGGGGATTGCCGGCGTGGTCAATCCCTGGTGGTGTGGGCAATCTCAGAGGGACGGCAAGCAGCTGCACAGGTTGACAACTACCTCTGCAAGGAAGAAGAAGACTACACAAACAGCAACGGGAGTCCTGAATCCGATCTCTTGAAGCGGCATCAGGAAATCAGCAAGAGCCATAAGACAGTTGCAAGCACACCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015930 GO:0016040 GO:0016053 GO:0016491 GO:0016638 GO:0016639 GO:0019676 GO:0019740 GO:0019752 GO:0032502 GO:0040007 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045181 GO:0046394 GO:0046686 GO:0048589 GO:0050896 GO:0055114 GO:0060359 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00250 ko00910 ko01100 ko01110 ko01120 ko01130 ko01230 map00250 map00910 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

Fer4_20 GATase_2 GXGXG Glu_syn_central Glu_synthase Pyr_redox_2

Protein Analysis

1904

Amino Acids

208.83

Weight (kDa)

5.8

Isoelectric Point (pI)

32.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	1 - 114	3e-46	Glutamine amidotransferases class-II
GATase_2	PF00310	142 - 229	8.6e-34	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	279 - 567	6.4e-112	Glutamate synthase central domain
Glu_synthase	PF01645	634 - 1002	4.2e-150	Conserved region in glutamate synthase
GXGXG	PF01493	1086 - 1271	1.2e-88	GXGXG motif
Fer4_20	PF14691	1407 - 1518	5.6e-22	Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
Pyr_redox_2	PF07992	1531 - 1857	1.1e-27	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	1535 - 1570	1.6e-08	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53460 AT5G53460 AT5G53460
fragaria_vesca	FvH4_7g31770 FvH4_7g31770 FvH4_7g31770
malus_domestica	MD01G1219500.v1.1 MD07G1290500.v1.1
prunus_persica	Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1
pyrus_communis	pycom01g22930 pycom07g26320
rosa_chinensis	RchiOBHm_Chr1g0381001
rosa_laevigata	RLG00000026253
rosa_multiflora	Rmu_ssc0000032.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278870
rosa_rugosa	Rorug01G0426400
rosa_samantha	Rh1AG450100 Rh1BG406100 Rh1CG420100 Rh1DG436600
rosa_wichuraiana	Rw1G039220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	2077, 2257, 3124
AccB1I	GGYRCC	5 cut(s)	829, 2824, 3545, 4561, 5140
AccB7I	CCANNNNNTGG	2 cut(s)	320, 3175
AccI	GTMKAC	2 cut(s)	546, 3051
AccII	CGCG	6 cut(s)	1221, 1272, 1405, 1438, 4183, 4285
AccIII	TCCGGA	1 cut(s)	4009
AciI	CCGC	9 cut(s)	1221, 1436, 1955, 2731, 2787, 2857, 3119, 4759, 5665
AclI	AACGTT	1 cut(s)	3313
AcoI	YGGCCR	8 cut(s)	525, 2148, 2728, 2752, 2778, 3870, 4773, 5156
AcsI	RAATTY	8 cut(s)	2024, 2046, 2413, 3356, 3755, 3911, 5104, 5333
AcuI	CTGAAG	9 cut(s)	234, 258, 857, 1113, 1284, 1725, 1910, 2568, 3156
AcvI	CACGTG	1 cut(s)	68
AdeI	CACNNNGTG	2 cut(s)	4332, 4463
AflII	CTTAAG	3 cut(s)	80, 2501, 4061
AflIII	ACRYGT	3 cut(s)	1333, 2941, 5302
AhlI	ACTAGT	1 cut(s)	3551
AjiI	CACGTC	1 cut(s)	1044
AjuI	GAANNNNNNNTTGG	2 cut(s)	5426, 5458
AleI	CACNNNNGTG	1 cut(s)	1301
AloI	GAACNNNNNNTCC	2 cut(s)	5061, 5093
Alw21I	GWGCWC	4 cut(s)	283, 711, 1675, 3949
Alw44I	GTGCAC	2 cut(s)	1671, 3945
AlwNI	CAGNNNCTG	2 cut(s)	437, 1841
Ama87I	CYCGRG	4 cut(s)	1812, 4412, 4988, 5414
Aor13HI	TCCGGA	1 cut(s)	4009
ApaLI	GTGCAC	2 cut(s)	1671, 3945
ApoI	RAATTY	8 cut(s)	2024, 2046, 2413, 3356, 3755, 3911, 5104, 5333
ArsI	GACNNNNNNTTYG	2 cut(s)	4526, 4558
AseI	ATTAAT	1 cut(s)	2489
Asp700I	GAANNNNTTC	1 cut(s)	3330
AspLEI	GCGC	4 cut(s)	1401, 4183, 4285, 4883
AsuC2I	CCSGG	3 cut(s)	486, 2355, 4163
AsuII	TTCGAA	1 cut(s)	3007
AsuNHI	GCTAGC	1 cut(s)	4627
AvaI	CYCGRG	4 cut(s)	1812, 4412, 4988, 5414
AvaII	GGWCC	8 cut(s)	748, 1064, 1124, 2646, 3166, 4259, 4897, 5411
AxyI	CCTNAGG	1 cut(s)	3959
BaeGI	GKGCMC	3 cut(s)	1675, 3949, 5143
BalI	TGGCCA	6 cut(s)	527, 2150, 2754, 2780, 3872, 5158
BamHI	GGATCC	2 cut(s)	265, 4204
BanI	GGYRCC	5 cut(s)	829, 2824, 3545, 4561, 5140
BanII	GRGCYC	3 cut(s)	711, 2524, 5360
BauI	CACGAG	1 cut(s)	1773
BbrPI	CACGTG	1 cut(s)	68
BbsI	GAAGAC	4 cut(s)	2009, 2481, 5503, 5625
Bbv12I	GWGCWC	4 cut(s)	283, 711, 1675, 3949
BbvCI	CCTCAGC	1 cut(s)	2970
BceAI	ACGGC	5 cut(s)	658, 2838, 4583, 4772, 5588
BcgI	CGANNNNNNTGC	2 cut(s)	2192, 2226
BclI	TGATCA	3 cut(s)	3640, 3988, 4639
BcnI	CCSGG	3 cut(s)	486, 2355, 4163
BcuI	ACTAGT	1 cut(s)	3551
BfaI	CTAG	8 cut(s)	188, 1382, 1737, 2571, 3552, 4134, 4628, 5393
BfmI	CTRYAG	4 cut(s)	568, 1503, 3066, 3222
BfrI	CTTAAG	3 cut(s)	80, 2501, 4061
BfuAI	ACCTGC	3 cut(s)	2077, 2257, 3124
BglI	GCCNNNNNGGC	2 cut(s)	4624, 5164
BglII	AGATCT	1 cut(s)	3787
BlpI	GCTNAGC	1 cut(s)	1431
BmcAI	AGTACT	3 cut(s)	1595, 2910, 3882
Bme18I	GGWCC	8 cut(s)	748, 1064, 1124, 2646, 3166, 4259, 4897, 5411
BmeT110I	CYCGRG	4 cut(s)	1812, 4412, 4988, 5414
BmgBI	CACGTC	1 cut(s)	1044
BmrI	ACTGGG	4 cut(s)	2109, 2640, 4338, 4596
BmtI	GCTAGC	1 cut(s)	4631
BmuI	ACTGGG	4 cut(s)	2109, 2640, 4338, 4596
BoxI	GACNNNNGTC	1 cut(s)	3129
BpiI	GAAGAC	4 cut(s)	2009, 2481, 5503, 5625
BplI	GAGNNNNNCTC	6 cut(s)	2192, 2224, 3378, 3410, 3956, 3988
BpmI	CTGGAG	5 cut(s)	540, 786, 1809, 2623, 2726
Bpu10I	CCTNAGC	4 cut(s)	2340, 2970, 3777, 5121
Bpu1102I	GCTNAGC	1 cut(s)	1431
Bpu14I	TTCGAA	1 cut(s)	3007
BpuEI	CTTGAG	6 cut(s)	200, 695, 1415, 3434, 4562, 5312
BpuMI	CCSGG	3 cut(s)	486, 2355, 4163
Bsa29I	ATCGAT	1 cut(s)	5458
BsaAI	YACGTR	2 cut(s)	68, 5303
BsaBI	GATNNNNATC	2 cut(s)	2122, 4983
BsaI	GGTCTC	2 cut(s)	1235, 2687
BsaWI	WCCGGW	1 cut(s)	4009
BsaXI	ACNNNNNCTCC	4 cut(s)	994, 1024, 1867, 1897
Bse118I	RCCGGY	2 cut(s)	2648, 5529
Bse21I	CCTNAGG	1 cut(s)	3959
Bse3DI	GCAATG	7 cut(s)	2112, 2151, 2397, 3079, 3271, 3439, 5403
Bse8I	GATNNNNATC	2 cut(s)	2122, 4983
BseAI	TCCGGA	1 cut(s)	4009
BseCI	ATCGAT	1 cut(s)	5458
BseJI	GATNNNNATC	2 cut(s)	2122, 4983
BseMI	GCAATG	7 cut(s)	2112, 2151, 2397, 3079, 3271, 3439, 5403
BseRI	GAGGAG	7 cut(s)	1253, 2266, 2987, 3980, 4400, 4865, 5378
BseSI	GKGCMC	3 cut(s)	1675, 3949, 5143
BseYI	CCCAGC	3 cut(s)	2663, 4622, 5519
BsgI	GTGCAG	7 cut(s)	1497, 1854, 2820, 3126, 3390, 4440, 5568
Bsh1236I	CGCG	6 cut(s)	1221, 1272, 1405, 1438, 4183, 4285
Bsh1285I	CGRYCG	1 cut(s)	485
BshNI	GGYRCC	5 cut(s)	829, 2824, 3545, 4561, 5140
BshVI	ATCGAT	1 cut(s)	5458
BsiEI	CGRYCG	1 cut(s)	485
BsiHKAI	GWGCWC	4 cut(s)	283, 711, 1675, 3949
BsiHKCI	CYCGRG	4 cut(s)	1812, 4412, 4988, 5414
BsiSI	CCGG	8 cut(s)	485, 2355, 2613, 2649, 2928, 4010, 4162, 5530
BslFI	GGGAC	8 cut(s)	87, 734, 2445, 3748, 4105, 4910, 5301, 5583
BsmBI	CGTCTC	1 cut(s)	1115
BsmFI	GGGAC	8 cut(s)	87, 734, 2445, 3748, 4105, 4910, 5301, 5583
BsmI	GAATGC	4 cut(s)	393, 1623, 2512, 2665
Bso31I	GGTCTC	2 cut(s)	1235, 2687
BsoBI	CYCGRG	4 cut(s)	1812, 4412, 4988, 5414
Bsp119I	TTCGAA	1 cut(s)	3007
Bsp1286I	GDGCHC	7 cut(s)	283, 711, 1675, 2524, 3949, 5143, 5360
Bsp13I	TCCGGA	1 cut(s)	4009
Bsp1407I	TGTACA	1 cut(s)	399
Bsp1720I	GCTNAGC	1 cut(s)	1431
Bsp19I	CCATGG	5 cut(s)	314, 817, 2672, 3169, 5083
BspACI	CCGC	9 cut(s)	1221, 1436, 1955, 2731, 2787, 2857, 3119, 4759, 5665
BspDI	ATCGAT	1 cut(s)	5458
BspEI	TCCGGA	1 cut(s)	4009
BspFNI	CGCG	6 cut(s)	1221, 1272, 1405, 1438, 4183, 4285
BspHI	TCATGA	4 cut(s)	733, 2455, 2494, 3298
BspMAI	CTGCAG	1 cut(s)	572
BspMI	ACCTGC	3 cut(s)	2077, 2257, 3124
BspOI	GCTAGC	1 cut(s)	4631
BspQI	GCTCTTC	1 cut(s)	3240
BspT104I	TTCGAA	1 cut(s)	3007
BspT107I	GGYRCC	5 cut(s)	829, 2824, 3545, 4561, 5140
BspTI	CTTAAG	3 cut(s)	80, 2501, 4061
BspTNI	GGTCTC	2 cut(s)	1235, 2687
BsrDI	GCAATG	7 cut(s)	2112, 2151, 2397, 3079, 3271, 3439, 5403
BsrFI	RCCGGY	2 cut(s)	2648, 5529
BsrGI	TGTACA	1 cut(s)	399
BssAI	RCCGGY	2 cut(s)	2648, 5529
BssSI	CACGAG	1 cut(s)	1773
Bst2BI	CACGAG	1 cut(s)	1773
Bst6I	CTCTTC	6 cut(s)	2229, 2764, 2799, 3240, 4017, 4121
BstAFI	CTTAAG	3 cut(s)	80, 2501, 4061
BstAPI	GCANNNNNTGC	1 cut(s)	5204
BstAUI	TGTACA	1 cut(s)	399
BstBAI	YACGTR	2 cut(s)	68, 5303
BstBI	TTCGAA	1 cut(s)	3007
BstDSI	CCRYGG	7 cut(s)	314, 817, 2011, 2672, 3169, 4565, 5083
BstEII	GGTNACC	1 cut(s)	3304
BstENI	CCTNNNNNAGG	5 cut(s)	1249, 3962, 4464, 4847, 5606
BstFNI	CGCG	6 cut(s)	1221, 1272, 1405, 1438, 4183, 4285
BstHHI	GCGC	4 cut(s)	1401, 4183, 4285, 4883
BstMCI	CGRYCG	1 cut(s)	485
BstNSI	RCATGY	4 cut(s)	1529, 2594, 2945, 5198
BstPAI	GACNNNNGTC	1 cut(s)	3129
BstPI	GGTNACC	1 cut(s)	3304
BstSFI	CTRYAG	4 cut(s)	568, 1503, 3066, 3222
BstSLI	GKGCMC	3 cut(s)	1675, 3949, 5143
BstUI	CGCG	6 cut(s)	1221, 1272, 1405, 1438, 4183, 4285
BstV2I	GAAGAC	4 cut(s)	2009, 2481, 5503, 5625
BstX2I	RGATCY	7 cut(s)	265, 1254, 1539, 2902, 3787, 4204, 5224
BstXI	CCANNNNNNTGG	2 cut(s)	2750, 3383
BstYI	RGATCY	7 cut(s)	265, 1254, 1539, 2902, 3787, 4204, 5224
Bsu15I	ATCGAT	1 cut(s)	5458
Bsu36I	CCTNAGG	1 cut(s)	3959
BsuTUI	ATCGAT	1 cut(s)	5458
BtgI	CCRYGG	7 cut(s)	314, 817, 2011, 2672, 3169, 4565, 5083
BtrI	CACGTC	1 cut(s)	1044
BtsI	GCAGTG	1 cut(s)	2418
BveI	ACCTGC	3 cut(s)	2077, 2257, 3124
CaiI	CAGNNNCTG	2 cut(s)	437, 1841
CciI	TCATGA	4 cut(s)	733, 2455, 2494, 3298
CfoI	GCGC	4 cut(s)	1401, 4183, 4285, 4883
Cfr10I	RCCGGY	2 cut(s)	2648, 5529
ClaI	ATCGAT	1 cut(s)	5458
CseI	GACGC	3 cut(s)	453, 2521, 3839
CsiI	ACCWGGT	1 cut(s)	4255
CspCI	CAANNNNNGTGG	4 cut(s)	1993, 2028, 5481, 5516
DraI	TTTAAA	3 cut(s)	2029, 3015, 4086
DraIII	CACNNNGTG	2 cut(s)	4332, 4463
EaeI	YGGCCR	8 cut(s)	525, 2148, 2728, 2752, 2778, 3870, 4773, 5156
Eam1104I	CTCTTC	6 cut(s)	2229, 2764, 2799, 3240, 4017, 4121
EarI	CTCTTC	6 cut(s)	2229, 2764, 2799, 3240, 4017, 4121
Ecl136II	GAGCTC	1 cut(s)	709
Eco147I	AGGCCT	2 cut(s)	3971, 4132
Eco24I	GRGCYC	3 cut(s)	711, 2524, 5360
Eco31I	GGTCTC	2 cut(s)	1235, 2687
Eco32I	GATATC	1 cut(s)	1647
Eco47I	GGWCC	8 cut(s)	748, 1064, 1124, 2646, 3166, 4259, 4897, 5411
Eco53kI	GAGCTC	1 cut(s)	709
Eco57I	CTGAAG	9 cut(s)	234, 258, 857, 1113, 1284, 1725, 1910, 2568, 3156
Eco72I	CACGTG	1 cut(s)	68
Eco81I	CCTNAGG	1 cut(s)	3959
Eco88I	CYCGRG	4 cut(s)	1812, 4412, 4988, 5414
Eco91I	GGTNACC	1 cut(s)	3304
EcoICRI	GAGCTC	1 cut(s)	709
EcoNI	CCTNNNNNAGG	5 cut(s)	1249, 3962, 4464, 4847, 5606
EcoO109I	RGGNCCY	4 cut(s)	748, 1124, 2634, 4897
EcoO65I	GGTNACC	1 cut(s)	3304
EcoRI	GAATTC	1 cut(s)	2413
EcoRV	GATATC	1 cut(s)	1647
EcoT22I	ATGCAT	1 cut(s)	4051
EcoT38I	GRGCYC	3 cut(s)	711, 2524, 5360
Esp3I	CGTCTC	1 cut(s)	1115
FalI	AAGNNNNNCTT	4 cut(s)	567, 599, 1113, 1145
FaqI	GGGAC	8 cut(s)	87, 734, 2445, 3748, 4105, 4910, 5301, 5583
FauI	CCCGC	1 cut(s)	4752
FbaI	TGATCA	3 cut(s)	3640, 3988, 4639
FblI	GTMKAC	2 cut(s)	546, 3051
FriOI	GRGCYC	3 cut(s)	711, 2524, 5360
FspBI	CTAG	8 cut(s)	188, 1382, 1737, 2571, 3552, 4134, 4628, 5393
GlaI	GCGC	4 cut(s)	1400, 4182, 4284, 4882
GsaI	CCCAGC	3 cut(s)	2667, 4626, 5523
GsuI	CTGGAG	5 cut(s)	540, 786, 1809, 2623, 2726
HapII	CCGG	8 cut(s)	485, 2355, 2613, 2649, 2928, 4010, 4162, 5530
HgaI	GACGC	3 cut(s)	453, 2521, 3839
HhaI	GCGC	4 cut(s)	1401, 4183, 4285, 4883
Hin6I	GCGC	4 cut(s)	1399, 4181, 4283, 4881
HinP1I	GCGC	4 cut(s)	1399, 4181, 4283, 4881
HincII	GTYRAC	5 cut(s)	52, 3052, 4765, 4789, 5593
HindII	GTYRAC	5 cut(s)	52, 3052, 4765, 4789, 5593
HindIII	AAGCTT	4 cut(s)	962, 1686, 3862, 4969
HpaI	GTTAAC	2 cut(s)	52, 4765
HpaII	CCGG	8 cut(s)	485, 2355, 2613, 2649, 2928, 4010, 4162, 5530
Hpy99I	CGWCG	1 cut(s)	5425
HspAI	GCGC	4 cut(s)	1399, 4181, 4283, 4881
KflI	GGGWCCC	1 cut(s)	748
Kpn2I	TCCGGA	1 cut(s)	4009
KroI	GCCGGC	1 cut(s)	5529
KroNI	GCCGGC	1 cut(s)	5531
Ksp22I	TGATCA	3 cut(s)	3640, 3988, 4639
KspAI	GTTAAC	2 cut(s)	52, 4765
LguI	GCTCTTC	1 cut(s)	3240
LmnI	GCTCC	9 cut(s)	161, 310, 455, 2218, 3386, 4012, 4012, 5110, 5446
MabI	ACCWGGT	1 cut(s)	4255
MaeI	CTAG	8 cut(s)	188, 1382, 1737, 2571, 3552, 4134, 4628, 5393
MfeI	CAATTG	4 cut(s)	132, 1014, 2469, 5370
MflI	RGATCY	7 cut(s)	265, 1254, 1539, 2902, 3787, 4204, 5224
MhlI	GDGCHC	7 cut(s)	283, 711, 1675, 2524, 3949, 5143, 5360
MlsI	TGGCCA	6 cut(s)	527, 2150, 2754, 2780, 3872, 5158
MluNI	TGGCCA	6 cut(s)	527, 2150, 2754, 2780, 3872, 5158
MlyI	GAGTC	4 cut(s)	822, 3814, 4401, 5650
MmeI	TCCRAC	8 cut(s)	975, 1447, 2866, 3156, 3262, 4535, 4588, 4792
Mox20I	TGGCCA	6 cut(s)	527, 2150, 2754, 2780, 3872, 5158
Mph1103I	ATGCAT	1 cut(s)	4051
MroI	TCCGGA	1 cut(s)	4009
MroNI	GCCGGC	1 cut(s)	5529
MroXI	GAANNNNTTC	1 cut(s)	3330
MscI	TGGCCA	6 cut(s)	527, 2150, 2754, 2780, 3872, 5158
MslI	CAYNNNNRTG	9 cut(s)	1046, 1301, 1530, 1791, 1899, 2055, 2156, 2852, 3648
Msp20I	TGGCCA	6 cut(s)	527, 2150, 2754, 2780, 3872, 5158
MspA1I	CMGCKG	1 cut(s)	5582
MspCI	CTTAAG	3 cut(s)	80, 2501, 4061
MspI	CCGG	8 cut(s)	485, 2355, 2613, 2649, 2928, 4010, 4162, 5530
MunI	CAATTG	4 cut(s)	132, 1014, 2469, 5370
Mva1269I	GAATGC	4 cut(s)	393, 1623, 2512, 2665
MvnI	CGCG	6 cut(s)	1221, 1272, 1405, 1438, 4183, 4285
NaeI	GCCGGC	1 cut(s)	5531
NciI	CCSGG	3 cut(s)	486, 2355, 4163
NcoI	CCATGG	5 cut(s)	314, 817, 2672, 3169, 5083
NgoMIV	GCCGGC	1 cut(s)	5529
NheI	GCTAGC	1 cut(s)	4627
NmeAIII	GCCGAG	1 cut(s)	4801
NmuCI	GTSAC	9 cut(s)	14, 499, 1039, 2378, 2991, 3304, 4666, 5045, 5450
NsiI	ATGCAT	1 cut(s)	4051
NspI	RCATGY	4 cut(s)	1529, 2594, 2945, 5198
NspV	TTCGAA	1 cut(s)	3007
OliI	CACNNNNGTG	1 cut(s)	1301
PaeI	GCATGC	2 cut(s)	2594, 5198
PaeR7I	CTCGAG	2 cut(s)	4412, 4988
PagI	TCATGA	4 cut(s)	733, 2455, 2494, 3298
PasI	CCCWGGG	1 cut(s)	2516
PceI	AGGCCT	2 cut(s)	3971, 4132
PciI	ACATGT	1 cut(s)	2941
PciSI	GCTCTTC	1 cut(s)	3240
PcsI	WCGNNNNNNNCGW	1 cut(s)	5176
PctI	GAATGC	4 cut(s)	393, 1623, 2512, 2665
PdiI	GCCGGC	1 cut(s)	5531
PdmI	GAANNNNTTC	1 cut(s)	3330
PflFI	GACNNNGTC	2 cut(s)	486, 746
PflMI	CCANNNNNTGG	2 cut(s)	320, 3175
PleI	GAGTC	4 cut(s)	821, 3814, 4401, 5649
PmaCI	CACGTG	1 cut(s)	68
PmlI	CACGTG	1 cut(s)	68
PpsI	GAGTC	4 cut(s)	821, 3814, 4401, 5649
Ppu21I	YACGTR	2 cut(s)	68, 5303
PpuMI	RGGWCCY	3 cut(s)	748, 1124, 4897
PscI	ACATGT	1 cut(s)	2941
PshAI	GACNNNNGTC	1 cut(s)	3129
PshBI	ATTAAT	1 cut(s)	2489
Psp124BI	GAGCTC	1 cut(s)	711
Psp1406I	AACGTT	1 cut(s)	3313
Psp5II	RGGWCCY	3 cut(s)	748, 1124, 4897
PspCI	CACGTG	1 cut(s)	68
PspEI	GGTNACC	1 cut(s)	3304
PspFI	CCCAGC	3 cut(s)	2663, 4622, 5519
PspPPI	RGGWCCY	3 cut(s)	748, 1124, 4897
PstI	CTGCAG	1 cut(s)	572
PstNI	CAGNNNCTG	2 cut(s)	437, 1841
PsuI	RGATCY	7 cut(s)	265, 1254, 1539, 2902, 3787, 4204, 5224
PsyI	GACNNNGTC	2 cut(s)	486, 746
PvuII	CAGCTG	1 cut(s)	5582
RseI	CAYNNNNRTG	9 cut(s)	1046, 1301, 1530, 1791, 1899, 2055, 2156, 2852, 3648
SacI	GAGCTC	1 cut(s)	711
SalI	GTCGAC	1 cut(s)	3050
SapI	GCTCTTC	1 cut(s)	3240
ScaI	AGTACT	3 cut(s)	1595, 2910, 3882
SchI	GAGTC	4 cut(s)	822, 3814, 4401, 5650
SduI	GDGCHC	7 cut(s)	283, 711, 1675, 2524, 3949, 5143, 5360
SexAI	ACCWGGT	1 cut(s)	4255
SfcI	CTRYAG	4 cut(s)	568, 1503, 3066, 3222
SfiI	GGCCNNNNNGGCC	1 cut(s)	5164
Sfr274I	CTCGAG	2 cut(s)	4412, 4988
SfuI	TTCGAA	1 cut(s)	3007
SinI	GGWCC	8 cut(s)	748, 1064, 1124, 2646, 3166, 4259, 4897, 5411
SlaI	CTCGAG	2 cut(s)	4412, 4988
SmiMI	CAYNNNNRTG	9 cut(s)	1046, 1301, 1530, 1791, 1899, 2055, 2156, 2852, 3648
SpeI	ACTAGT	1 cut(s)	3551
SphI	GCATGC	2 cut(s)	2594, 5198
SseBI	AGGCCT	2 cut(s)	3971, 4132
SsiI	CCGC	9 cut(s)	1221, 1436, 1955, 2731, 2787, 2857, 3119, 4759, 5665
SspI	AATATT	3 cut(s)	293, 659, 3149
SspMI	CTAG	8 cut(s)	188, 1382, 1737, 2571, 3552, 4134, 4628, 5393
SstI	GAGCTC	1 cut(s)	711
StuI	AGGCCT	2 cut(s)	3971, 4132
TaqII	GACCGA	1 cut(s)	1052
TauI	GCSGC	6 cut(s)	1438, 1958, 2733, 2789, 3122, 5668
TseFI	GTSAC	9 cut(s)	14, 499, 1039, 2378, 2991, 3304, 4666, 5045, 5450
Tsp45I	GTSAC	9 cut(s)	14, 499, 1039, 2378, 2991, 3304, 4666, 5045, 5450
TspGWI	ACGGA	3 cut(s)	2000, 2903, 3378
Tth111I	GACNNNGTC	2 cut(s)	486, 746
Van91I	CCANNNNNTGG	2 cut(s)	320, 3175
Vha464I	CTTAAG	3 cut(s)	80, 2501, 4061
VneI	GTGCAC	2 cut(s)	1671, 3945
VpaK11BI	GGWCC	8 cut(s)	748, 1064, 1124, 2646, 3166, 4259, 4897, 5411
VspI	ATTAAT	1 cut(s)	2489
XagI	CCTNNNNNAGG	5 cut(s)	1249, 3962, 4464, 4847, 5606
XapI	RAATTY	8 cut(s)	2024, 2046, 2413, 3356, 3755, 3911, 5104, 5333
XceI	RCATGY	4 cut(s)	1529, 2594, 2945, 5198
XcmI	CCANNNNNNNNNTGG	5 cut(s)	1579, 2425, 2762, 3880, 5151
XhoI	CTCGAG	2 cut(s)	4412, 4988
XmiI	GTMKAC	2 cut(s)	546, 3051
XmnI	GAANNNNTTC	1 cut(s)	3330
XspI	CTAG	8 cut(s)	188, 1382, 1737, 2571, 3552, 4134, 4628, 5393
ZrmI	AGTACT	3 cut(s)	1595, 2910, 3882
Zsp2I	ATGCAT	1 cut(s)	4051

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.