Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0008640

Physical Location & Seq

Genomic Coordinates Forward (+)

35109790 .. 35120053

Gene Structure 10264 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1290500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6564 bp

ATGTTGGCGAGCACTGGCGGTTCCGTCGTTCAGCTCCGAACCAAACCGTCGCTCCTGCCGTCCTGCTCTCCTTCCCGGAAATCATGCAAGGCTCTGGCCAAGAAGTTCTTCGGGACCAGATTGAGGGCGTCCGGATCCGAGAGGCTCCATTTGTGGCGCTCCGACGGTCCCGGTCGCTCGCCGAAGCTCCGAGTCGTCGTGCGCAACATGCTCTCCGCCGTTCCAGAGAAGCCCCTCGGGCTATACGACCCGTCGTTCGATAAGGACTCGTGTGGTGTCGGCTTCGTCGCCGAATTGTCCGGCGAAGGCAGCCGCAAAACGATTACGGATGCAATTGAAATGCTGGTGCGGATGACGCACAGAGGAGCGTGTGGGTGTGAAACGAATACCGGTGATGGAGCTGGGATTCTCGTCGGTGTTCCGCACGACTTCTACAAGGAGGTAACCAAAGATGCCGGGTTTGATATTCCACCGGCAGGGGAGTACGCTGTTGGAATGTTCTTCTTGCCCACATCTGAGAGCCGAAGAGAAGAAAGCAAAAGAGTATTTGCAAAGGTTGCAGAGTCCCTTGGACACACTGTTCTGGGGTGGCGATCTGTTCCAACAGATAACTCAGACTTGGGGAATTCTGCTTTGCAGACTGAACCTGTTATTGAGCAAGTGTTCCTTACTGCGACCCCAAAGTCAAAAGTTGATTTCGAACGACAGATGTACATACTAAGAAGGCTGTCAATGGTTGCTATCCGAGCTGCATTAAACCTTGAACACGGTGGTGCTAAGGACTTTTATATATGTTCGCTGTCCTCAAGGACTGTTGTTTACAAAGGTCAGCTGAAGCCCGTTCAGCTGAAGGGTTATTATTATGCAGATCTTGGCAATGAAAGGTTTACGAGCTACATGGCCCTGGTACACTCTCGATTCTCAACAAATACCTTTCCCAGCTGGGATCGTGCTCAGCCTATGCGTGTCATTGGCCACAATGGTGAAATCAACACCCTCAGAGGAAATGTTAACTGGATGAAGGCACGCGAGGGACTGCTGAAGTGTAAGGAACTTGGTCTATCAGAAAATGATTTAAAAAAACTTTTACCCATTGTGGACGCCAGTTCTTCAGATTCAGGAGCTTTTGATGCTGTCCTTGAACTTCTGGTTCAAGCTGGAAGAAGCCTCCCCGAAGCTATCATGATGATGATTCCTGAAGCATGGCAAAATGACAAGAACATGGACCCGGATAGGAAAGCCCTGTATGAGTATTTCTCATCTCTCATGGAGCCATGGGATGGGCCAGCTCTCATATCATTTACTGATGGCCGCTATTTGGGAGCTACACTGGATCGTAATGGACTCAGACCTGGTCGATTTTACGTCACTCACAGCGGACGGGTTATTATGGCCAGTGAAGTTGGCGTCGTAGACATTCCACCGGAAGATGTCAGCAGGAAAGGAAGACTTAACCCTGGAATGATGCTTTTGGTGGACTTTGAGAACCACGTTGTGGTGGATGATGAAGCGTTGAAGCAGCAATATTCTTTGGCAAGGCCGTATGCGGAGTGGCTTCAAAGGCAAAAGATTGAGCTCAAGGACATTGTAGATTCTGTTCATGAATCTGACAGGGTCCCTCCGTCCATAGCTGGAGTTATTCCTGCATCTACTAATGATGAAACCATGGAAAACATGGGAATCCATGGTTTATTGGCACCGCTGAAAGCTTTTGGTTATACAGTTGAATCCTTGGAAATGTTGTTGCTTCCCATGGCAAAAGATGGCGTAGAGGCTCTTGGGTCGATGGGAAATGATACCCCATTGGCTGTTATGTCTAAGAGAGAAAAGCTTACCTTTGAGTATTTCAAGCAAATGTTTGCTCAAGTAACAAACCCTCCAATTGACCCCATTCGAGAGAAGGTGGTCACTTCTATGGAATGCATGATTGGTCCAGAGGGTGCTTTGACAGAAACCACCGAAGAACAATGTCATCGTCTCTCACTCAAAGGTCCTCTTTTAACCATTGATGAAATGGAAGCAATCAAGAAAATGAACTATAGAGGTTGGCGCTGTAAAGTTCTTGATATAACTTATTCCAAGGGACGTGGTAGGGAAGGATTGGAGGAAACCTTGGATAGGATTTGTTCTGACGCACATGAAGCAATAAAGAAGGGATACACCACTCTTGTTCTTTCAGACAGAGCCTTCTCCCCAAAGCGCGTTGCAGTAAGCTCCCTCTTGGCTGTTGGCGCTGTTCATCAACATCTAGTTAAAAACCTTGAACGCACACAAGTGGGGTTGATAATTGAATCTGCTGAGCCACGTGAAGTTCACCATTTCTGTACACTGGTTGGTTTTGGTGCAGATGCTATCTGCCCTTACTTGGCTATAGAGGCCATTTGGAGGCTGCAGGTGGACGGAAAGATTCCACCAATAGCTAATGGTGCAATATACTCCAAAGATGAATTGGTTAAAAAGTACTTCAAAGCAAGCACATATGGAATGATGAAGGTTCTTGCCAAGATGGGGATTTCTACTTTGGCCTCTTACAAGGGTGCACAGATTTTTGAAGCTTTGGGTCTTTCATCTGAAGTAATTGAGAGGTGCTTTGCAGGAACCCCTAGTAGAGTTGAGGGTGCAACATTCGAGATGCTAGCTCACGACGAACTTCATATGCACGAATTGGCATTTCCCTCTCGAAGCTATCCTCCAGGAAGTGCAGAAGCTGTAGCACTACCAAATCCTGGGGATTACCATTGGAGAAAAGGTGGTGAGGTACACCTAAACGATCCTGTTGCTATAGCAAAACTTCAAGAGGCTGCCAGAACTAACAGTGTTGCTGCATATAAAGAATACTCCAAGCTTATTCATGAATTAAATAAAGCCTGCAATCTCCGCGGTCTTTTGAGATTTAAAAACACAGAGCAGCAGATTCACTTGGATGAAGTGGAACCTGCTAGCGAGATTGTTAAACGGTTCTGTACTGGTGCTATGAGTTATGGATCAATATCATTGGAAGCGCATAGTACACTGGCCATTGCTATGAACAGAATTGGAGGAAAGTCAAATACAGGTGAGGGAGGTGAGCAGCCATCTCGTATGGAGCCTCTTCCAGATGGTTCAAGGAATCCAAAGAGGAGTGCAATTAAGCAGGTTGCCAGTGGGAGATTTGGAGTTTCAAGTTACTATCTGACGAATGCTGATGAACTCCAGATAAAAATGGCTCAGGGTGCCAAGCCTGGTGAAGGAGGTGAGCTTCCTGGACACAAGGTTATTGGAGACATTGCTGTCACCAGGAATTCTACTGCTGGTGTGGGTCTTATCAGTCCACCTCCCCATCATGATATCTACTCGATTGAAGACCTTGCCCAATTAATTCATGATCTTAAGAATGCTAACCCAGCAGCTCGAATTAGTGTCAAGTTGGTATCGGAAGCAGGTGTGGGAGTAGTTGCTAGTGGAGTTGTGAAGGGGCATGCTGATCATGTTTTGATCTCTGGACACGATGGAGGCACAGGGGCCTCTAGATGGACAGGAATTAAGAATGCAGGGCTTCCATGGGAACTTGGTTTGGCTGAGACTCATCAAACCTTGGTTTCTAATGATCTTCGTGGCCGCACTACTCTCCAGACTGATGGCCAACTAAAAACTGGAAGAGATGTTGCCATAGCTACACTTCTTGGTGCGGAAGAGTTTGGCTTCAGCACTGCACCTCTCATAACCCTTGGCTGCATTATGATGCGGAAGTGCCACAAGAACACATGTCCTGTTGGTATCGCAACTCAAGATCCAGTACTTCGTGAGAAGTTTGCTGGAGAACCTGAACATGTTATTAATTTTTTCTTTATGATTGCTGAGGAACTCAGAGAGATAATGGCGCAGCTTGGTTTCCGTACCATAAATGAGATGGTTGGTCGTTCAGATATGCTTGAAGTTGATAGAGAAGTGACTAAAAACAATGAGAAGCTTGACAATATTGATCTATCTTTGTTACTTCGACCTGCCGCAGACCTTCGGCCTGGAGCTGCACAATATTGTGTTGAGAAACAAGACCATGGCTTGGATATGGCTCTTGATCACAAGCTAATTTCTATGTCTAAAGCTGCTTTGGAAAAAGCTCTTCCTGTATATTTTGAGACACCAATTTGCAATGTGAACCGTGCCGTTGGTACAATGCTTAGCCACGAGGTGACAAAACGTTACAACAGGGCTGGGCTTCCTGCTGACACCATTCATATCAAATTTAGTGGAAGTGGAGGACAGAGTCTTGGAGCATTTTTGTGCTCTGGAATCATGCTTGAGCTTGAGGGTGACAGTAATGATTATGTTGGTAAAGGTTTGTCTGGTGGAAAGATTGTAGTTTATCCTCCCAAGGGAAGCAGGTTTGACCCAAAAGAAAATATTGTCATCGGTAATGTAGCTTTATATGGTGCCACTAGTGGTGAGGCATACTTCAATGGAATGGCAGCAGAGAGATTTTGTGTTCGCAATTCGGGGGCTAAGGCAGTTGTCGAGGGTGTTGGTGATCATGGCTGTGAATACATGACAGGTGGCACTGTTGTTGTGCTCGGGAAAACTGGCAGGAACTTTGCTGCTGGTATGAGTGGTGGTATTGCCTATGTTTATGACGTGGATGGACAATTTCGGTCTCGATGTAATCCTGAGCTTGTAGATCTTGATACACTTGAAGAAGAAGATATTCTGACTCTTCAAATGATGATACAACAACATCAACGTCACACAAACAGCCTGCTGGCCGTTCAAGTACTGGCTGATTTTGAAAATCTTCTGCCTAAGTTTATCAAGGTTATCCCAAGAGAGTACAAGCGAGTTCTTGCAGATATGAAAGAGGAAACCAAACAGGTTATAGAGCACAAGGAGGAAGATGAGCCAGAGCTTGAAGAGAAAGATGCCTTTCAAGAACTTAAGAAGTTGGCGGCTGCATCTTTAAATGGGAAATCCAATCAGAAGGTAGAAGATGCTGAAGCATTGAAAAGGCCTTCTCAGGTCACTGATGCTGTTAAACATCGTGGTTTTATTTATTATGAGCGCGAGGGCGTTCAATATAGGGATCCCAATGTTCGGATGAATGACTGGAAGGAAGTTATGGAGGAAACTAAACCTGGACCACTTGTGAAGACTCAGTCAGCCCGTTGCATGGACTGCGGGACTCCTTTCTGCCATCAGGAGAACTCTGGATGCCCTCTTGGTAACAAAATACCAGAGTTCAATGAGTTGGTGTACCAGAATAGGTGGCATGAAGCATTAGAGAGACTTCTCGAGACAAATAACTTCCCTGAGTTTACTGGTCGTGTTTGTCCAGCACCATGTGAAGGTTCTTGTGTTCTGGGTATTATTGAGAATCCTGTATCTATCAAAAGCATAGAATGTGCCATCATAGACAAGGCTTTTGAGGAAGGTTGGATGGTGCCACGACCTCCCGTAAAGAGAACTGGGAAAAGGGTTGCCATTGTAGGAAGCGGGCCAGCTGGTCTAGCTGCTGCTGATCAGCTAAACAGAATAGGCCACACAGTGACTGTGTATGAGCGTGCTGATAGAATTGGAGGGCTTATGATGTATGGAGTGCCTAATATGAAGACTGATAAAAAGGAGATAGTTCAACGGCGGGTTAACCTTATGGCTGAGGAAGGTGTCAACTTTGTGGTTAATGCTAATATTGGAAACGATCCTTTGTACTCGCTTGATCGCCTTCGAGAGGAGAACAATGCCATTGTTTTAGCAGTAGGAGCAACAAAACCAAGGGACCTTCCCGTACCAGGGCGAGAGCTGTCAGGAGTACATTTTGCTATGGAATTTCTTCATGCAAACACCAAAAGCTTACTAGATAGCAATCTTGAGGATGGTAACTACATCTCTGCCAAGGGCAAGAAAGTTGTGGTAATTGGTGGAGGTGACACTGGCACTGATTGCATTGGAACATCCGTTCGCCATGGCTGCACTAACATCATAAATTTGGAGCTTCTTCCTGAGCCACCACGAAAAAGGGCTCCAGGAAACCCTTGGCCACAGTGGCCTCGTGTATTCCGTGTGGATTATGGGCACCAAGAAGTTGCAGCTAAGTTCGGCAAAGATCCAAGAACTTATGAAGTGCTGACAAAGAGATTTGTGGGAGATGAGAATGGGGCCTTGAAAGGACTCGAGGTGGTCCGTGTCAAATGGGAGAAGGATGAAACCGGGCGGTTCCAATTCAACGAAATCGAGGGATCTGAGGAGATCCTCGAGGCTGACCTCGTCCTTCTCGCAATGGGGTTCCTTGGACCAGAGGCGACGGTAGCAGAGAAGTTGGGTTTGGAACGTGACCAACGCTCAAACTACAAGGCGGACTATGGTCGGTTCTCAACCAATGTGGATGGGGTATTTGCCGCTGGGGATTGCCGGCGCGGTCAGTCCCTTGTGGTGTGGGCAATCTCAGAGGGAAGGCAAGTCGCTGCACAGGTTGACAAGTACCTCTCGAAGGAAGAAGAAGACCACGCCATCAGCAATGGGAGCCATCAAAATGTTGGCAAGAGGCACCGAGACCTTAACCCAACAGGCACCAGCAAACACACAGTGATGACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015930 GO:0016040 GO:0016053 GO:0016491 GO:0016638 GO:0016639 GO:0019676 GO:0019740 GO:0019752 GO:0032502 GO:0040007 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045181 GO:0046394 GO:0046686 GO:0048589 GO:0050896 GO:0055114 GO:0060359 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00250 ko00910 ko01100 ko01110 ko01120 ko01130 ko01230 map00250 map00910 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

Fer4_20 GATase_2 GXGXG Glu_syn_central Glu_synthase Pyr_redox_2

Protein Analysis

2188

Amino Acids

240.43

Weight (kDa)

6.21

Isoelectric Point (pI)

35.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	91 - 518	8.3e-184	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	568 - 856	9.7e-112	Glutamate synthase central domain
Glu_synthase	PF01645	923 - 1291	4e-150	Conserved region in glutamate synthase
GXGXG	PF01493	1375 - 1560	1.5e-87	GXGXG motif
Fer4_20	PF14691	1690 - 1801	4.8e-22	Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
Pyr_redox_2	PF07992	1815 - 2140	5.7e-26	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	1818 - 1853	1.7e-08	NAD(P)-binding Rossmann-like domain
Amino_oxidase	PF01593	1824 - 1852	9.1e-06	Flavin containing amine oxidoreductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0008640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53460 AT5G53460 AT5G53460
fragaria_vesca	FvH4_7g31770 FvH4_7g31770 FvH4_7g31770
malus_domestica	MD01G1219500.v1.1 MD07G1290500.v1.1
prunus_persica	Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1
pyrus_communis	pycom01g22930 pycom07g26320
rosa_chinensis	RchiOBHm_Chr1g0381001
rosa_laevigata	RLG00000026253
rosa_multiflora	Rmu_ssc0000032.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278870
rosa_rugosa	Rorug01G0426400
rosa_samantha	Rh1AG450100 Rh1BG406100 Rh1CG420100 Rh1DG436600
rosa_wichuraiana	Rw1G039220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	2383, 3410
AasI	GACNNNNNNGTC	2 cut(s)	682, 3269
AbsI	CCTCGAGG	1 cut(s)	6221
Acc16I	TGCGCA	1 cut(s)	203
Acc36I	ACCTGC	6 cut(s)	2383, 2944, 3124, 3410, 3991, 4353
AccB1I	GGYRCC	7 cut(s)	1696, 3212, 4412, 5410, 6042, 6513, 6536
AccB7I	CCANNNNNTGG	4 cut(s)	1280, 1495, 2726, 4042
AccI	GTMKAC	1 cut(s)	1413
AccII	CGCG	5 cut(s)	1029, 2202, 2880, 5034, 6384
AccIII	TCCGGA	1 cut(s)	131
AclI	AACGTT	1 cut(s)	4180
AcoI	YGGCCR	9 cut(s)	96, 973, 1309, 1392, 3015, 3595, 3619, 4737, 6005
AcsI	RAATTY	5 cut(s)	625, 3280, 4223, 5795, 5953
AcuI	CTGAAG	8 cut(s)	854, 869, 1061, 1095, 1218, 2592, 3667, 4986
AcvI	CACGTG	1 cut(s)	2306
AcyI	GRCGYC	3 cut(s)	128, 1101, 1407
AdeI	CACNNNGTG	5 cut(s)	1495, 4171, 5312, 5515, 6553
AflII	CTTAAG	2 cut(s)	3368, 4907
AflIII	ACRYGT	2 cut(s)	3743, 3808
AgeI	ACCGGT	1 cut(s)	389
AhlI	ACTAGT	1 cut(s)	4418
AjiI	CACGTC	2 cut(s)	2087, 4612
AjuI	GAANNNNNNNTTGG	6 cut(s)	92, 124, 1911, 1943, 6275, 6307
AleI	CACNNNNGTG	3 cut(s)	771, 981, 2273
AloI	GAACNNNNNNTCC	4 cut(s)	564, 596, 5910, 5942
Alw21I	GWGCWC	7 cut(s)	14, 955, 1578, 2542, 4268, 4551, 4857
Alw26I	GTCTC	8 cut(s)	1982, 3255, 3554, 4112, 4635, 5248, 5258, 6513
Alw44I	GTGCAC	1 cut(s)	2538
AlwNI	CAGNNNCTG	2 cut(s)	2708, 5519
Ama87I	CYCGRG	5 cut(s)	236, 4550, 5261, 6140, 6221
Aor13HI	TCCGGA	1 cut(s)	131
ApaLI	GTGCAC	1 cut(s)	2538
ApoI	RAATTY	5 cut(s)	625, 3280, 4223, 5795, 5953
ArsI	GACNNNNNNTTYG	4 cut(s)	239, 271, 5375, 5407
AseI	ATTAAT	2 cut(s)	3356, 3816
AsiGI	ACCGGT	1 cut(s)	389
Asp700I	GAANNNNTTC	4 cut(s)	107, 4680, 4767, 5799
AspLEI	GCGC	9 cut(s)	159, 204, 2052, 2202, 2234, 3004, 3862, 5034, 6384
AsuC2I	CCSGG	5 cut(s)	76, 171, 457, 1229, 6178
AsuII	TTCGAA	1 cut(s)	699
AsuNHI	GCTAGC	2 cut(s)	2635, 2939
AvaI	CYCGRG	5 cut(s)	236, 4550, 5261, 6140, 6221
BaeGI	GKGCMC	2 cut(s)	2542, 6045
BalI	TGGCCA	6 cut(s)	98, 975, 1394, 3017, 3621, 6007
BamHI	GGATCC	2 cut(s)	134, 5053
BanI	GGYRCC	7 cut(s)	1696, 3212, 4412, 5410, 6042, 6513, 6536
BanII	GRGCYC	2 cut(s)	1578, 5992
BauI	CACGAG	3 cut(s)	268, 4166, 6018
BbrPI	CACGTG	1 cut(s)	2306
BbsI	GAAGAC	5 cut(s)	1453, 3348, 5126, 5585, 6474
Bbv12I	GWGCWC	7 cut(s)	14, 955, 1578, 2542, 4268, 4551, 4857
BbvCI	CCTCAGC	2 cut(s)	3837, 5625
BceAI	ACGGC	6 cut(s)	43, 203, 1525, 4130, 4724, 5621
BcgI	CGANNNNNNTGC	4 cut(s)	2759, 2793, 3059, 3093
BciVI	GTATCC	1 cut(s)	2150
BclI	TGATCA	4 cut(s)	3463, 4057, 4507, 5488
BcnI	CCSGG	5 cut(s)	76, 171, 457, 1229, 6178
BcoDI	GTCTC	8 cut(s)	1982, 3255, 3554, 4112, 4635, 5248, 5258, 6513
BcuI	ACTAGT	1 cut(s)	4418
BfaI	CTAG	9 cut(s)	2249, 2604, 2636, 2940, 3438, 3507, 4419, 5477, 5825
BfmI	CTRYAG	5 cut(s)	2038, 2370, 2390, 2709, 2781
BfoI	RGCGCY	3 cut(s)	160, 2053, 2235
BfrI	CTTAAG	2 cut(s)	3368, 4907
BfuAI	ACCTGC	6 cut(s)	2383, 2944, 3124, 3410, 3991, 4353
BfuI	GTATCC	1 cut(s)	2150
BglI	GCCNNNNNGGC	2 cut(s)	238, 6013
BglII	AGATCT	2 cut(s)	868, 4654
BlpI	GCTNAGC	3 cut(s)	954, 2298, 4160
BmcAI	AGTACT	3 cut(s)	2462, 3777, 4749
BmeT110I	CYCGRG	5 cut(s)	236, 4550, 5261, 6140, 6221
BmgBI	CACGTC	2 cut(s)	2087, 4612
BmrI	ACTGGG	1 cut(s)	5445
BmtI	GCTAGC	2 cut(s)	2639, 2943
BmuI	ACTGGG	1 cut(s)	5445
BoxI	GACNNNNGTC	1 cut(s)	6330
BpiI	GAAGAC	5 cut(s)	1453, 3348, 5126, 5585, 6474
BplI	GAGNNNNNCTC	2 cut(s)	1241, 1273
BpmI	CTGGAG	7 cut(s)	1653, 2676, 3176, 3593, 3816, 4023, 5976
Bpu10I	CCTNAGC	7 cut(s)	777, 3207, 3837, 4482, 4644, 5625, 5970
Bpu1102I	GCTNAGC	3 cut(s)	954, 2298, 4160
Bpu14I	TTCGAA	1 cut(s)	699
BpuEI	CTTGAG	7 cut(s)	790, 1562, 1848, 3750, 4301, 4307, 5858
BpuMI	CCSGG	5 cut(s)	76, 171, 457, 1229, 6178
BsaAI	YACGTR	1 cut(s)	2306
BsaBI	GATNNNNATC	2 cut(s)	2989, 5832
BsaHI	GRCGYC	3 cut(s)	128, 1101, 1407
BsaI	GGTCTC	2 cut(s)	4635, 6513
BsaWI	WCCGGW	3 cut(s)	131, 389, 1423
BsaXI	ACNNNNNCTCC	8 cut(s)	36, 66, 197, 227, 1861, 1891, 2734, 2764
Bse118I	RCCGGY	3 cut(s)	389, 472, 6378
Bse3DI	GCAATG	6 cut(s)	883, 3018, 3264, 4138, 6252, 6490
Bse8I	GATNNNNATC	2 cut(s)	2989, 5832
BseAI	TCCGGA	1 cut(s)	131
BseJI	GATNNNNATC	2 cut(s)	2989, 5832
BseMI	GCAATG	6 cut(s)	883, 3018, 3264, 4138, 6252, 6490
BseRI	GAGGAG	4 cut(s)	378, 3133, 5714, 6227
BseSI	GKGCMC	2 cut(s)	2542, 6045
BseYI	CCCAGC	6 cut(s)	401, 938, 942, 3382, 4193, 6368
BsgI	GTGCAG	6 cut(s)	2364, 2721, 3675, 3993, 5923, 6417
Bsh1236I	CGCG	5 cut(s)	1029, 2202, 2880, 5034, 6384
Bsh1285I	CGRYCG	1 cut(s)	175
BshNI	GGYRCC	7 cut(s)	1696, 3212, 4412, 5410, 6042, 6513, 6536
BshTI	ACCGGT	1 cut(s)	389
BsiEI	CGRYCG	1 cut(s)	175
BsiHKAI	GWGCWC	7 cut(s)	14, 955, 1578, 2542, 4268, 4551, 4857
BsiHKCI	CYCGRG	5 cut(s)	236, 4550, 5261, 6140, 6221
BslFI	GGGAC	9 cut(s)	127, 153, 550, 1047, 1601, 2097, 5164, 5759, 6376
BsmAI	GTCTC	8 cut(s)	1982, 3255, 3554, 4112, 4635, 5248, 5258, 6513
BsmBI	CGTCTC	1 cut(s)	1982
BsmFI	GGGAC	9 cut(s)	127, 153, 550, 1047, 1601, 2097, 5164, 5759, 6376
BsmI	GAATGC	4 cut(s)	1925, 3184, 3379, 3532
Bso31I	GGTCTC	2 cut(s)	4635, 6513
BsoBI	CYCGRG	5 cut(s)	236, 4550, 5261, 6140, 6221
Bsp119I	TTCGAA	1 cut(s)	699
Bsp1286I	GDGCHC	9 cut(s)	14, 955, 1578, 2542, 4268, 4551, 4857, 5992, 6045
Bsp13I	TCCGGA	1 cut(s)	131
Bsp1407I	TGTACA	2 cut(s)	711, 2324
Bsp1720I	GCTNAGC	3 cut(s)	954, 2298, 4160
Bsp19I	CCATGG	7 cut(s)	1274, 1665, 1684, 1752, 3539, 4036, 5932
BspEI	TCCGGA	1 cut(s)	131
BspFNI	CGCG	5 cut(s)	1029, 2202, 2880, 5034, 6384
BspHI	TCATGA	5 cut(s)	1182, 1600, 2851, 3322, 3361
BspMAI	CTGCAG	1 cut(s)	2394
BspMI	ACCTGC	6 cut(s)	2383, 2944, 3124, 3410, 3991, 4353
BspOI	GCTAGC	2 cut(s)	2639, 2943
BspQI	GCTCTTC	1 cut(s)	4107
BspT104I	TTCGAA	1 cut(s)	699
BspT107I	GGYRCC	7 cut(s)	1696, 3212, 4412, 5410, 6042, 6513, 6536
BspTI	CTTAAG	2 cut(s)	3368, 4907
BspTNI	GGTCTC	2 cut(s)	4635, 6513
BsrDI	GCAATG	6 cut(s)	883, 3018, 3264, 4138, 6252, 6490
BsrFI	RCCGGY	3 cut(s)	389, 472, 6378
BsrGI	TGTACA	2 cut(s)	711, 2324
BssAI	RCCGGY	3 cut(s)	389, 472, 6378
BssNI	GRCGYC	3 cut(s)	128, 1101, 1407
BssSI	CACGAG	3 cut(s)	268, 4166, 6018
Bst2BI	CACGAG	3 cut(s)	268, 4166, 6018
Bst6I	CTCTTC	7 cut(s)	520, 3096, 3631, 3666, 4107, 4695, 4878
BstACI	GRCGYC	3 cut(s)	128, 1101, 1407
BstAFI	CTTAAG	2 cut(s)	3368, 4907
BstAPI	GCANNNNNTGC	3 cut(s)	557, 5145, 5910
BstAUI	TGTACA	2 cut(s)	711, 2324
BstBAI	YACGTR	1 cut(s)	2306
BstBI	TTCGAA	1 cut(s)	699
BstDSI	CCRYGG	8 cut(s)	1274, 1665, 1684, 1752, 2878, 3539, 4036, 5932
BstEII	GGTNACC	1 cut(s)	442
BstENI	CCTNNNNNAGG	4 cut(s)	2116, 3225, 5696, 6455
BstFNI	CGCG	5 cut(s)	1029, 2202, 2880, 5034, 6384
BstH2I	RGCGCY	3 cut(s)	160, 2053, 2235
BstHHI	GCGC	9 cut(s)	159, 204, 2052, 2202, 2234, 3004, 3862, 5034, 6384
BstMAI	GTCTC	8 cut(s)	1982, 3255, 3554, 4112, 4635, 5248, 5258, 6513
BstMCI	CGRYCG	1 cut(s)	175
BstNSI	RCATGY	4 cut(s)	211, 3461, 3747, 3812
BstPAI	GACNNNNGTC	1 cut(s)	6330
BstPI	GGTNACC	1 cut(s)	442
BstSFI	CTRYAG	5 cut(s)	2038, 2370, 2390, 2709, 2781
BstSLI	GKGCMC	2 cut(s)	2542, 6045
BstUI	CGCG	5 cut(s)	1029, 2202, 2880, 5034, 6384
BstV2I	GAAGAC	5 cut(s)	1453, 3348, 5126, 5585, 6474
BstX2I	RGATCY	8 cut(s)	134, 868, 3769, 4654, 5053, 6073, 6206, 6216
BstXI	CCANNNNNNTGG	1 cut(s)	3617
BstYI	RGATCY	8 cut(s)	134, 868, 3769, 4654, 5053, 6073, 6206, 6216
BsuI	GTATCC	1 cut(s)	2150
BtgI	CCRYGG	8 cut(s)	1274, 1665, 1684, 1752, 2878, 3539, 4036, 5932
BtrI	CACGTC	2 cut(s)	2087, 4612
BtsI	GCAGTG	1 cut(s)	3687
BveI	ACCTGC	6 cut(s)	2383, 2944, 3124, 3410, 3991, 4353
CaiI	CAGNNNCTG	2 cut(s)	2708, 5519
CciI	TCATGA	5 cut(s)	1182, 1600, 2851, 3322, 3361
CfoI	GCGC	9 cut(s)	159, 204, 2052, 2202, 2234, 3004, 3862, 5034, 6384
Cfr10I	RCCGGY	3 cut(s)	389, 472, 6378
Cfr42I	CCGCGG	1 cut(s)	2881
CseI	GACGC	5 cut(s)	117, 364, 1109, 1396, 2141
CsiI	ACCWGGT	1 cut(s)	1351
CspAI	ACCGGT	1 cut(s)	389
CspCI	CAANNNNNGTGG	6 cut(s)	2068, 2103, 4210, 4245, 6330, 6365
DraI	TTTAAA	3 cut(s)	1077, 2896, 4932
DraIII	CACNNNGTG	5 cut(s)	1495, 4171, 5312, 5515, 6553
DrdI	GACNNNNNNGTC	2 cut(s)	682, 3269
DseDI	GACNNNNNNGTC	2 cut(s)	682, 3269
EaeI	YGGCCR	9 cut(s)	96, 973, 1309, 1392, 3015, 3595, 3619, 4737, 6005
Eam1104I	CTCTTC	7 cut(s)	520, 3096, 3631, 3666, 4107, 4695, 4878
EarI	CTCTTC	7 cut(s)	520, 3096, 3631, 3666, 4107, 4695, 4878
EciI	GGCGGA	2 cut(s)	205, 6338
Ecl136II	GAGCTC	1 cut(s)	1576
Eco147I	AGGCCT	1 cut(s)	4981
Eco24I	GRGCYC	2 cut(s)	1578, 5992
Eco31I	GGTCTC	2 cut(s)	4635, 6513
Eco32I	GATATC	1 cut(s)	3328
Eco53kI	GAGCTC	1 cut(s)	1576
Eco57I	CTGAAG	8 cut(s)	854, 869, 1061, 1095, 1218, 2592, 3667, 4986
Eco72I	CACGTG	1 cut(s)	2306
Eco88I	CYCGRG	5 cut(s)	236, 4550, 5261, 6140, 6221
Eco91I	GGTNACC	1 cut(s)	442
EcoICRI	GAGCTC	1 cut(s)	1576
EcoNI	CCTNNNNNAGG	4 cut(s)	2116, 3225, 5696, 6455
EcoO109I	RGGNCCY	5 cut(s)	1615, 1991, 3501, 5746, 6126
EcoO65I	GGTNACC	1 cut(s)	442
EcoRI	GAATTC	2 cut(s)	625, 3280
EcoRV	GATATC	1 cut(s)	3328
EcoT22I	ATGCAT	1 cut(s)	1925
EcoT38I	GRGCYC	2 cut(s)	1578, 5992
Esp3I	CGTCTC	1 cut(s)	1982
FaqI	GGGAC	9 cut(s)	127, 153, 550, 1047, 1601, 2097, 5164, 5759, 6376
FauI	CCCGC	3 cut(s)	5141, 5456, 5601
FauNDI	CATATG	2 cut(s)	2479, 2655
FbaI	TGATCA	4 cut(s)	3463, 4057, 4507, 5488
FblI	GTMKAC	1 cut(s)	1413
FriOI	GRGCYC	2 cut(s)	1578, 5992
FspBI	CTAG	9 cut(s)	2249, 2604, 2636, 2940, 3438, 3507, 4419, 5477, 5825
FspI	TGCGCA	1 cut(s)	203
GlaI	GCGC	9 cut(s)	158, 203, 2051, 2201, 2233, 3003, 3861, 5033, 6383
GsaI	CCCAGC	6 cut(s)	405, 942, 946, 3386, 4197, 6372
GsuI	CTGGAG	7 cut(s)	1653, 2676, 3176, 3593, 3816, 4023, 5976
HaeII	RGCGCY	3 cut(s)	160, 2053, 2235
HgaI	GACGC	5 cut(s)	117, 364, 1109, 1396, 2141
HhaI	GCGC	9 cut(s)	159, 204, 2052, 2202, 2234, 3004, 3862, 5034, 6384
Hin1I	GRCGYC	3 cut(s)	128, 1101, 1407
Hin6I	GCGC	9 cut(s)	157, 202, 2050, 2200, 2232, 3002, 3860, 5032, 6382
HinP1I	GCGC	9 cut(s)	157, 202, 2050, 2200, 2232, 3002, 3860, 5032, 6382
HincII	GTYRAC	4 cut(s)	1012, 5614, 5638, 6442
HindII	GTYRAC	4 cut(s)	1012, 5614, 5638, 6442
HindIII	AAGCTT	6 cut(s)	1707, 1829, 2553, 2843, 3947, 5818
HpaI	GTTAAC	2 cut(s)	1012, 5614
HpyCH4IV	ACGT	8 cut(s)	1365, 1491, 2086, 2305, 4180, 4611, 4717, 6298
HpySE526I	ACGT	8 cut(s)	1365, 1491, 2086, 2305, 4180, 4611, 4717, 6298
Hsp92I	GRCGYC	3 cut(s)	128, 1101, 1407
HspAI	GCGC	9 cut(s)	157, 202, 2050, 2200, 2232, 3002, 3860, 5032, 6382
KflI	GGGWCCC	1 cut(s)	1615
Kpn2I	TCCGGA	1 cut(s)	131
KroI	GCCGGC	1 cut(s)	6378
KroNI	GCCGGC	1 cut(s)	6380
Ksp22I	TGATCA	4 cut(s)	3463, 4057, 4507, 5488
KspAI	GTTAAC	2 cut(s)	1012, 5614
KspI	CCGCGG	1 cut(s)	2881
LguI	GCTCTTC	1 cut(s)	4107
MabI	ACCWGGT	1 cut(s)	1351
MaeI	CTAG	9 cut(s)	2249, 2604, 2636, 2940, 3438, 3507, 4419, 5477, 5825
MaeII	ACGT	8 cut(s)	1365, 1491, 2086, 2305, 4180, 4611, 4717, 6298
MfeI	CAATTG	2 cut(s)	333, 1881
MflI	RGATCY	8 cut(s)	134, 868, 3769, 4654, 5053, 6073, 6206, 6216
MhlI	GDGCHC	9 cut(s)	14, 955, 1578, 2542, 4268, 4551, 4857, 5992, 6045
MlsI	TGGCCA	6 cut(s)	98, 975, 1394, 3017, 3621, 6007
MluNI	TGGCCA	6 cut(s)	98, 975, 1394, 3017, 3621, 6007
MmeI	TCCRAC	4 cut(s)	186, 472, 626, 5384
Mox20I	TGGCCA	6 cut(s)	98, 975, 1394, 3017, 3621, 6007
Mph1103I	ATGCAT	1 cut(s)	1925
MroI	TCCGGA	1 cut(s)	131
MroNI	GCCGGC	1 cut(s)	6378
MroXI	GAANNNNTTC	4 cut(s)	107, 4680, 4767, 5799
MscI	TGGCCA	6 cut(s)	98, 975, 1394, 3017, 3621, 6007
Msp20I	TGGCCA	6 cut(s)	98, 975, 1394, 3017, 3621, 6007
MspA1I	CMGCKG	8 cut(s)	832, 847, 942, 1377, 1702, 2880, 5471, 6368
MspCI	CTTAAG	2 cut(s)	3368, 4907
MunI	CAATTG	2 cut(s)	333, 1881
Mva1269I	GAATGC	4 cut(s)	1925, 3184, 3379, 3532
MvnI	CGCG	5 cut(s)	1029, 2202, 2880, 5034, 6384
NaeI	GCCGGC	1 cut(s)	6380
NciI	CCSGG	5 cut(s)	76, 171, 457, 1229, 6178
NcoI	CCATGG	7 cut(s)	1274, 1665, 1684, 1752, 3539, 4036, 5932
NdeI	CATATG	2 cut(s)	2479, 2655
NgoMIV	GCCGGC	1 cut(s)	6378
NheI	GCTAGC	2 cut(s)	2635, 2939
NsbI	TGCGCA	1 cut(s)	203
NsiI	ATGCAT	1 cut(s)	1925
NspI	RCATGY	4 cut(s)	211, 3461, 3747, 3812
NspV	TTCGAA	1 cut(s)	699
OliI	CACNNNNGTG	3 cut(s)	771, 981, 2273
PaeI	GCATGC	1 cut(s)	3461
PaeR7I	CTCGAG	3 cut(s)	5261, 6140, 6221
PagI	TCATGA	5 cut(s)	1182, 1600, 2851, 3322, 3361
PaqCI	CACCTGC	2 cut(s)	2383, 3410
PceI	AGGCCT	1 cut(s)	4981
PciI	ACATGT	2 cut(s)	3743, 3808
PciSI	GCTCTTC	1 cut(s)	4107
PcsI	WCGNNNNNNNCGW	3 cut(s)	56, 243, 6025
PctI	GAATGC	4 cut(s)	1925, 3184, 3379, 3532
PdiI	GCCGGC	1 cut(s)	6380
PdmI	GAANNNNTTC	4 cut(s)	107, 4680, 4767, 5799
PflFI	GACNNNGTC	5 cut(s)	1353, 1613, 4245, 5125, 6233
PflMI	CCANNNNNTGG	4 cut(s)	1280, 1495, 2726, 4042
PfoI	TCCNGGA	4 cut(s)	74, 2692, 3241, 5992
PinAI	ACCGGT	1 cut(s)	389
PmaCI	CACGTG	1 cut(s)	2306
PmlI	CACGTG	1 cut(s)	2306
Ppu21I	YACGTR	1 cut(s)	2306
PpuMI	RGGWCCY	3 cut(s)	1615, 1991, 5746
PscI	ACATGT	2 cut(s)	3743, 3808
PshAI	GACNNNNGTC	1 cut(s)	6330
PshBI	ATTAAT	2 cut(s)	3356, 3816
Psp124BI	GAGCTC	1 cut(s)	1578
Psp1406I	AACGTT	1 cut(s)	4180
Psp5II	RGGWCCY	3 cut(s)	1615, 1991, 5746
PspCI	CACGTG	1 cut(s)	2306
PspEI	GGTNACC	1 cut(s)	442
PspFI	CCCAGC	6 cut(s)	401, 938, 942, 3382, 4193, 6368
PspPPI	RGGWCCY	3 cut(s)	1615, 1991, 5746
PspXI	VCTCGAGB	2 cut(s)	6140, 6221
PsrI	GAACNNNNNNTAC	2 cut(s)	1581, 1613
PstI	CTGCAG	1 cut(s)	2394
PstNI	CAGNNNCTG	2 cut(s)	2708, 5519
PsuI	RGATCY	8 cut(s)	134, 868, 3769, 4654, 5053, 6073, 6206, 6216
PsyI	GACNNNGTC	5 cut(s)	1353, 1613, 4245, 5125, 6233
PvuII	CAGCTG	4 cut(s)	832, 847, 942, 5471
SacI	GAGCTC	1 cut(s)	1578
SacII	CCGCGG	1 cut(s)	2881
SapI	GCTCTTC	1 cut(s)	4107
ScaI	AGTACT	3 cut(s)	2462, 3777, 4749
SduI	GDGCHC	9 cut(s)	14, 955, 1578, 2542, 4268, 4551, 4857, 5992, 6045
SexAI	ACCWGGT	1 cut(s)	1351
SfcI	CTRYAG	5 cut(s)	2038, 2370, 2390, 2709, 2781
SfiI	GGCCNNNNNGGCC	1 cut(s)	6013
Sfr274I	CTCGAG	3 cut(s)	5261, 6140, 6221
Sfr303I	CCGCGG	1 cut(s)	2881
SfuI	TTCGAA	1 cut(s)	699
SgrBI	CCGCGG	1 cut(s)	2881
SlaI	CTCGAG	3 cut(s)	5261, 6140, 6221
SpeI	ACTAGT	1 cut(s)	4418
SphI	GCATGC	1 cut(s)	3461
SseBI	AGGCCT	1 cut(s)	4981
SspI	AATATT	5 cut(s)	1526, 3958, 4016, 4384, 5659
SspMI	CTAG	9 cut(s)	2249, 2604, 2636, 2940, 3438, 3507, 4419, 5477, 5825
SstI	GAGCTC	1 cut(s)	1578
StuI	AGGCCT	1 cut(s)	4981
TaiI	ACGT	8 cut(s)	1368, 1494, 2089, 2308, 4183, 4614, 4720, 6301
TaqII	GACCGA	1 cut(s)	4617
TauI	GCSGC	6 cut(s)	315, 1314, 3600, 3989, 4922, 6368
TspGWI	ACGGA	8 cut(s)	13, 341, 1611, 2415, 3863, 5914, 6017, 6140
Tth111I	GACNNNGTC	5 cut(s)	1353, 1613, 4245, 5125, 6233
Van91I	CCANNNNNTGG	4 cut(s)	1280, 1495, 2726, 4042
Vha464I	CTTAAG	2 cut(s)	3368, 4907
VneI	GTGCAC	1 cut(s)	2538
VspI	ATTAAT	2 cut(s)	3356, 3816
XagI	CCTNNNNNAGG	4 cut(s)	2116, 3225, 5696, 6455
XapI	RAATTY	5 cut(s)	625, 3280, 4223, 5795, 5953
XbaI	TCTAGA	1 cut(s)	3506
XceI	RCATGY	4 cut(s)	211, 3461, 3747, 3812
XcmI	CCANNNNNNNNNTGG	7 cut(s)	1672, 2011, 2446, 3629, 3886, 6000, 6500
XhoI	CTCGAG	3 cut(s)	5261, 6140, 6221
XmiI	GTMKAC	1 cut(s)	1413
XmnI	GAANNNNTTC	4 cut(s)	107, 4680, 4767, 5799
XspI	CTAG	9 cut(s)	2249, 2604, 2636, 2940, 3438, 3507, 4419, 5477, 5825
ZrmI	AGTACT	3 cut(s)	2462, 3777, 4749
Zsp2I	ATGCAT	1 cut(s)	1925

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.