Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008640

Physical Location & Seq

Genomic Coordinates Reverse (-)

31171938 .. 31182759

Gene Structure 10822 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1219500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6627 bp

ATGTTGGCGAGCACTGGCGGTTCCGTCGTTCAGCTCCGAGCCAAATCGTCACTTCTGCCGTCGCAGCTCAATGCGACGCCGGTAGCTCGGCTCGGCGGGAGCAGGGCGGCGGTGACTACCTGCTCTGCTTCCCGGAAATCTAGCAAGGCTCTGGCGAAGAAGTTCTTCGGGACCCGATTGAGGGCGTCCGGATCCGAGAGGCTCCATTTGTGGCGCTCCGACGGTCCAGGTCGCTCTCCGAAGCTCCGAGTCGTCGTACGCAACATGCTCTCCGCCGTTCCAGAGAAGCCCCTCGGGCTATACGACCCGTCGTTTGATAAGGACTCGTGTGGTGTCGGCTTCGTCGCCGAATTGTCCGGCGAGGTCAGCCGCAAAACGATTACGGATGCAATTGAAATGTTGGAGCGGATGACGCACAGAGGAGCGTGTGGGTGCGAAACGAATACTGGTGATGGAGCTGGGATTCTCGTCGGTGTTCCTCACGACTTCTACAAGGAGGTTACCAAAGATGCCGGGTTTGATCTTCCCCCGGCAGGGGAATACGCCGTTGGAATGTTCTTCTTGCCTGCATCTGAGAGTCGAAGAGAAGAAAGCAAAAGAGTATTTGCAAAGGTTGCAGAGTCCCTTGGACACACTGTTCTTGGGTGGCGATCTGTCCCAACAGATAACTCGGACTTGGGGAAGTCTGCCTTGCAGACAGAACCTGTTATTGAGCAAGTGTTCCTTACTGCGACCCCAAGGTCAAAACTTGACCTCGAGCGACAGATGTACATATTAAGAAGGCTGTCAATGGTTGCTATCCGAGCTGCATTAAACCTTGAACTTGGTGGCGCTAAAGACTTTTATATATGTTCGCTTTCCTCAAGAACTGTTGTGTACAAGGGTCAGCTGAAGCCCATTCAGCTGAAGGATTACTATTATGCAGATCTTGGCAATGAAAGGTTTACGAGCTACATGGCCCTGGTTCACTCTCGATTCTCAACAAATACCTTTCCCAGCTGGGATCGCGCTCAGCCTATGCGTATTATTGGCCACAATGGTGAAATCAACACCCTCAGAGGAAATGTTAACTGGATGAAGGCACGTGAGGGACTGCTTAAGTGTAAGGAACTTGGTCTATCAGAAAATGATTTAAAGAAGCTGTTACCCATTGTGGATGCCAGTTCTTCGGATTCAGGTGCTTTTGATGGTGTTCTTGAGCTTCTGGTTCAAGCTGGAAGAAGTCTCCCTGAAGCTATTATGATGTTGATTCCTGAAGCATGGCAAAATGACAAGAACATGGACCCAGATAGGAAAGCACTGTATGAGTATTTCTCATCTCTCATGGAGCCATGGGATGGGCCAGCTCTTATATCATTTACTGATGGACGCTATTTGGGAGCTACACTGGATCGTAATGGACTCCGACCTGGTCGATTTTACGTCACCCACAGTGGACGCGTTATTATGGCCAGTGAAGTTGGCGTAGTGGACATTCCACCGGAAGATGTCAGCAGGAAAGGAAGACTTAACCCTGGAATGATGCTTTTGGTGGACTTTGAGAACCACGTTGTTGTGGACGACGAAGCCTTGAAGCAGCAATATTCCTTGGCAAGGCCGTATGGGGAGTGGCTTCAAAGGCAAAAGATTGAGCTCAAGGACATTGTAGATTCTGTTCATGAATCTGACAGGGTCCCTCCGTCCATAGCTGGAGCTATTCCTGCATCTACTGATGATGAAACCATGGAAAACATGGGAATCCACGGTTTATTGGCACCACTGAAAGCTTTTGGTTATACAGTTGAATCCTTGGAAATGTTGTTGCTTCCCATGGCAAAAGATGGTGTAGAGGCTCTTGGGTCGATGGGAAATGATACCCCGTTGGCTGTTATGTCTAATAGAGAAAAGCTTACATTTGAGTATTTCAAGCAAATGTTTGCTCAAGTAACAAACCCCCCAATTGATCCCATCCGAGAGAAGGTAGTAACTTCTATGGAATGCATGATTGGTCCAGAGGGTGATCTGACAGAAACCACCGAAGAGCAATGTCACCGTCTCTCACTCAAAGGTCCTCTTTTGACCATTGACGAAATGGAAGCAATCAAGCAAATGAACTATAGAGGTTGGCGCTGCAAAGTTCTTGATATAACTTATTCCAAGAAACGTGGTAGGGAAGGATTGGAGGAAACCTTGGACAGGATTTGTGCTGAAGCGCGTGAAGCAATAAAGAAGGGATATACCACACTTGTTCTTTCAGACAGAGCCTTCTCCCCAAAGCGTGTTGCAGTAAGCTCCCTCTTGGCTGTTGGCGCTGTTCATCAACATCTAGTCAAAAACCTTGAACGCACACAAGTGGGGTTGATAATTGAATCTGCTGAGCCACGTGAAGTTCATCATTTCTGTACACTGGTTGGCTTCGGTGCAGATGCTATCTGCCCATACTTGGCTATAGAGGCCATTTGGAGACTGCAGGTGGACGGAAAGATCCCACCAAAAGCTAATGGGGCAATATACTCCAAGGATGAACTGGTTAAAAAGTACTTCAAAGCAAGCAACTATGGAATGATGAAGGTTCTTGCCAAGATGGGGATATCTACTTTGGCCTCTTACAAGGGTGCACAGATTTTTGAAGCTTTGGGTCTTTCATCAGAAGTAGTTGAGAGGTGCTTTGCAGGAACCCCTAGTAGAGTTGAGGGTGCAACATTCGAGATGCTAGCTCGCGACGAACTTCATATGCACGAGTTGGCATTTCCCTCTCGAAGCTATCCTCCAGGAAGTGCCGAGGCAGTGGCACTGCCAAATCCTGGGGATTACCATTGGAGAAAAGGTGGTGAGGTTCACCTAAATGATCCTTTTGCTATAGCGAAGCTTCAAGAGGCTGCCAGAACTAACAGTGTTGCTGCATATAAAGAATACTCCAAGTTCATTCATGAATTAAATAAAGCCTGCAATCTCCGGGGTCTTTTGAAATTTAAAAACACAGAGCAGCAGATTCACTTGGATGAAGTGGAACCTGCTAGTGAGATTGTTAAACGGTTCTGTACCGGTGCTATGAGTTATGGATCAATATCATTGGAGGCGCATAGTACACTGGCCATTGCTATGAACAGAATTGGAGGAAAGTCAAATACAGGTGAGGGAGGCGAGCAACCATCTCGTATGGAGCCTCTTTCAGATGGTTCACGGAATCCAAAGAGGAGTGCAATTAAGCAGGTTGCCAGTGGGAGATTTGGAGTTTCAAGTTACTATCTGACGAATGCTGATGAACTGCAGATAAAAATGGCTCAGGGTGCCAAGCCTGGTGAAGGAGGTGAGCTTCCTGGACACAAGGTTATTGGAGACATTGCAGTTACGAGGAATTCTACTGCCGGTGTGGGTCTTATCAGTCCACCTCCCCATCATGATATCTACTCAATTGAAGACCTTGCGCAATTAATTCATGATCTTAAGAATGCTAACCCAGCAGCTCGAATTAGTGTCAAGTTGGTATCGGAGGCAGGTGTTGGAGTAGTTGCTAGTGGAGTTGTGAAGGGGCACGCAGACCATGTTTTGATCTCTGGACATGATGGAGGCACGGGGGCCTCTAGATGGACAGGAATTAAGAATGCAGGGCTTCCATGGGAACTTGGTTTGGCTGAGACCCATCAAACCTTGGTTGCTAATGATCTTCGTGGCCGGACAACTCTCCAGACTGATGGCCAACTAAAAACTGGAAGAGATGTTGCCATAGCTGCACTTCTTGGTGCGGAAGAGTTTGGCTTCAGCACTGCACCTCTCATAACCCTTGGCTGCATTATGATGCGGAAGTGCCACAAGAACACATGTCCTGTTGGCATCGCAACTCAAGATCCTGTACTTCGTGAGAAGTTTGCTGGAGAACCTGAACATGTTATTAATTTTTTCTTTATGATAGCTGAGGAACTCAGAGAGATTATGGCGCAGCTTGGTTTCCGTACTATAAATGAGATGGTTGGACGTTCAGATATGCTTGAAGTTGATAGAGAAGTGACTAAAAACAATGAGAAGCTTGACAATATTGATCTATCTTTGTTACTTCGACCTGCAGCAGACCTTCGGCCTGGAGCTGCACAATATTGTGTTGAGAAACAAGACCATGGCTTGGATATGGCTCTTGATCACAAGTTAATTGCTATGTCTAAAGCTGCTTTGGAAAAGGCTCTTCCTGTATATTTTGAGACACCAATTTGCAATGTGAACCGTGCTGTTGGAACAATGCTTAGCCACGAGGTGACAAAGCGTTACAACAGGGCAGGGCTTCCTGCTGACACCATTCATATCAAATTTAGTGGAAGTGCAGGGCAGAGTCTTGGAGCATTTTTGTGCCCTGGAATCATGCTTGAGCTTGAGGGTGACAGTAATGATTATGTTGGTAAAGGTTTGTCTGGTGGAAAGATTGTAGTTTATCCTCCCAAGGGAAGCAAGTTTGACCCAAAAGAAAATATTGTCATCGGTAATGTAGCTTTATATGGTGCCACTAGTGGTGAGGCATACTTCAATGGAATGGCAGCAGAAAGATTTTGTGTTCGCAATTCAGGGGCTAAGGCAGTTGTCGAGGGTGTTGGTGATCATGGCTGTGAATATATGACAGGTGGCACTGTTGTTGTGCTCGGGAAAACTGGCAGGAACTTTGCTGCTGGTATGAGTGGTGGCATTGCCTATGTTTATGATGTGGATGGACAATTTCGGTCTCGATGTAATCCTGAGCTTGTAGATCTTGATAGACTAGAAGAAGAAGATATTCTGACTCTTCAAATGATGATACAACAACATCAACGGCACACTAACAGCCTGCTGGCCATTGAAGTACTGGCTGATTTTGAAAATCTTTTGCCTAAGTTTATCAAGGTTATTCCAAGAGAGTACAAGCGTGTTCTTGCAGATATGAAAGAGGAAACCAAACAGGTTATAGAGCACGAGGAGGAAGATGAGCCAGAGCTTGAAGAGAAAGATGCTTTTGAAGAACTTAAGAAGTTGGCGGCTGCATCTTTAAATGGGAAATCCAATCAGGTAGAAGATGCTGAAGCATTGAAGAGGCCTTCTCAGGTCACTGATGCTGTTAAACATCGCGGTTTTATTTCTTATGAGCGCGAGGGCGTTCAATATAGGGATCCTAATGTTCGGATGAATGACTGGAAGGAAGTTATGGAGGAAACTAAACCTGGACCACTTGTGAAGACTCAGTCAGCCCGTTGCATGGATTGCGGGACTCCTTTCTGCCATCAGGAGAACACTGGATGTCCTCTTGGTAACAAAATACCAGAGTTCAATGAGTTGGTGTACCAGAATAGGTGGCATGAAGCATTAGAGAGACTTCTTGAGACCAATAACTTCCCCGAGTTTACTGGTCGGGTTTGTCCGGCACCATGTGAAGGTTCTTGTGTTCTGGGTATTATTGAGAATCCCGTATCTATCAAAAGCATAGAATGTGCCATCATAGACAAGGCTTTTGAGGAAGGGTGGATGGTGCCACGACCTCCCGTAAAGAGAACTGGGAAAAGGGTTGCCATTGTAGGAAGTGGGCCAGCTGGTCTAGCTGCTGCTGATCAGCTAAACAGAATAGGCCACACAGTGACTGTGTATGAGCGTGCTGATAGAATCGGAGGGCTCATGATGTATGGAGTTCCTAATATGAAGACTGATAAAGTGGAGATAGTTCAACGGCGGGTTAACCTTATGACTGAGGAAGGTGTCAACTTTGTGGTTAACGCTAATATTGGAAATGATCCTTTGTACTCACTTGAAAGGCTTCGAGAGGAGAACAATGCCATTGTTTTAGCTGTAGGAGCAACGAAACCAAGGGATCTTCCTGTACCAGGGAGAGAGCTGTCCGGAGTACATTTTGCAATGGAATTTCTACGTGCAAACACCAAGAGCTTATTAGATAGCAATCTCGAGGACGGTAACTACATTTCTGCCAAGGGGAAGAAAGTTGTGGTAATTGGCGGAGGTGACACTGGCACAGATTGCATTGGAACATCTGTTCGCCATGGCTGCACTAGCATCATAAATCTGGAGCTTCTCCCTGAGCCACCACGAACAAGGGCTCCAGGAAACCCTTGGCCACAGTGGCCTCGTGTATTCCGTGTGGATTATGGGCACCAAGAAGTTGCAGCTAAGTTTGGCAAAGATCCAAGAACTTATGAGGTGCTGACAAAGAGATTTGTGGGAGATGAGAATGGGGCCGTGAAAGGACTCGAGGTGGTACGTGTCAAATGGGAGAAGGATGAAACTGGGCGGTTCCAATTCAAGGAAATCGAGGGATCTGAGGAGATCCTCGAGGCTGACCTCGTCCTACTCGCAATGGGGTTCCTTGGACCGGAGGCGACGGTAGCAGAGAAGTTGGGTTTGGAACGTGACCAACGCTCAAACTACAAGGCGGACTATGGTCGGTTCTCAACCAATGTGGATGGGGTATTTGCCGCTGGGGATTGCCGGCGTGGTCAATCCCTTGTGGTGTGGGCAATCTCGGAGGGAAGGCAAGCCGCTGCACAGGTTGACAAATACCTCTCGAACGAGGAAGAAGACCGCACCATCAGCAACGGGAGCCACCCAGATCTTAGCAAGAGGCATCAAGACCTCAGCAAGAGAAACCCAACCGGTAGCAGTAAACACACAGTGATGACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015930 GO:0016040 GO:0016053 GO:0016491 GO:0016638 GO:0016639 GO:0019676 GO:0019740 GO:0019752 GO:0032502 GO:0040007 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045181 GO:0046394 GO:0046686 GO:0048589 GO:0050896 GO:0055114 GO:0060359 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00250 ko00910 ko01100 ko01110 ko01120 ko01130 ko01230 map00250 map00910 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

Fer4_20 GATase_2 GXGXG Glu_syn_central Glu_synthase Pyr_redox_2

Protein Analysis

2209

Amino Acids

242.61

Weight (kDa)

6.08

Isoelectric Point (pI)

35.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	110 - 537	9.2e-184	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	587 - 875	4.2e-113	Glutamate synthase central domain
Glu_synthase	PF01645	942 - 1310	1.2e-150	Conserved region in glutamate synthase
GXGXG	PF01493	1394 - 1579	8.3e-89	GXGXG motif
Fer4_20	PF14691	1708 - 1819	4.8e-22	Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
Pyr_redox_2	PF07992	1833 - 2158	4.4e-26	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	1836 - 1871	1.8e-08	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53460 AT5G53460 AT5G53460
fragaria_vesca	FvH4_7g31770 FvH4_7g31770 FvH4_7g31770
malus_domestica	MD01G1219500.v1.1 MD07G1290500.v1.1
prunus_persica	Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1
pyrus_communis	pycom01g22930 pycom07g26320
rosa_chinensis	RchiOBHm_Chr1g0381001
rosa_laevigata	RLG00000026253
rosa_multiflora	Rmu_ssc0000032.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278870
rosa_rugosa	Rorug01G0426400
rosa_samantha	Rh1AG450100 Rh1BG406100 Rh1CG420100 Rh1DG436600
rosa_wichuraiana	Rw1G039220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	2440, 3467
AasI	GACNNNNNNGTC	1 cut(s)	739
AbsI	CCTCGAGG	1 cut(s)	6275
Acc16I	TGCGCA	1 cut(s)	3408
Acc36I	ACCTGC	6 cut(s)	128, 2440, 3001, 3181, 3467, 4048
AccB1I	GGYRCC	6 cut(s)	1753, 3269, 4469, 5359, 5464, 6096
AccB7I	CCANNNNNTGG	3 cut(s)	1337, 2783, 4099
AccBSI	CCGCTC	1 cut(s)	406
AccII	CGCG	6 cut(s)	1008, 1440, 2194, 2700, 5067, 5088
AccIII	TCCGGA	2 cut(s)	188, 5828
AcoI	YGGCCR	7 cut(s)	1030, 1449, 3072, 3652, 3676, 4794, 6059
AcsI	RAATTY	4 cut(s)	2948, 3337, 4280, 5849
AcuI	CTGAAG	7 cut(s)	911, 926, 1251, 1275, 2208, 3724, 5040
AcvI	CACGTG	2 cut(s)	1085, 2363
AcyI	GRCGYC	2 cut(s)	77, 185
AdeI	CACNNNGTG	4 cut(s)	4228, 5366, 5569, 6616
AflII	CTTAAG	3 cut(s)	1097, 3425, 4964
AflIII	ACRYGT	4 cut(s)	1438, 3800, 3865, 6205
AgeI	ACCGGT	2 cut(s)	3023, 6596
AhlI	ACTAGT	1 cut(s)	4475
AjuI	GAANNNNNNNTTGG	6 cut(s)	531, 563, 1968, 2000, 6329, 6361
AleI	CACNNNNGTG	2 cut(s)	1038, 2330
AloI	GAACNNNNNNTCC	6 cut(s)	621, 653, 5964, 5996, 6028, 6060
Alw21I	GWGCWC	5 cut(s)	14, 1635, 2599, 4608, 4914
Alw26I	GTCTC	9 cut(s)	1229, 2039, 2437, 3312, 3611, 4169, 4692, 5302, 5312
Alw44I	GTGCAC	1 cut(s)	2595
AlwNI	CAGNNNCTG	2 cut(s)	704, 5573
Ama87I	CYCGRG	7 cut(s)	293, 755, 4607, 5333, 5891, 6194, 6275
Aor13HI	TCCGGA	2 cut(s)	188, 5828
ApaLI	GTGCAC	1 cut(s)	2595
ApoI	RAATTY	4 cut(s)	2948, 3337, 4280, 5849
ArsI	GACNNNNNNTTYG	4 cut(s)	296, 328, 5429, 5461
AseI	ATTAAT	2 cut(s)	3413, 3873
AsiGI	ACCGGT	2 cut(s)	3023, 6596
Asp700I	GAANNNNTTC	4 cut(s)	161, 164, 4737, 5745
AsuC2I	CCSGG	4 cut(s)	133, 514, 530, 2936
AsuNHI	GCTAGC	1 cut(s)	2692
AvaI	CYCGRG	7 cut(s)	293, 755, 4607, 5333, 5891, 6194, 6275
AvaII	GGWCC	8 cut(s)	171, 224, 1282, 1672, 1988, 2048, 5162, 6314
BaeGI	GKGCMC	4 cut(s)	2599, 3516, 4325, 6099
BalI	TGGCCA	6 cut(s)	1032, 1451, 3074, 3678, 4796, 6061
BamHI	GGATCC	2 cut(s)	191, 5107
BanI	GGYRCC	6 cut(s)	1753, 3269, 4469, 5359, 5464, 6096
BanII	GRGCYC	3 cut(s)	1635, 5607, 6046
BauI	CACGAG	5 cut(s)	325, 2717, 4223, 4913, 6072
BbrPI	CACGTG	2 cut(s)	1085, 2363
BbsI	GAAGAC	5 cut(s)	1510, 3405, 5180, 5639, 6528
Bbv12I	GWGCWC	5 cut(s)	14, 1635, 2599, 4608, 4914
BbvCI	CCTCAGC	2 cut(s)	3894, 6578
BceAI	ACGGC	7 cut(s)	43, 260, 530, 1582, 4790, 5675, 6167
BcgI	CGANNNNNNTGC	2 cut(s)	3116, 3150
BclI	TGATCA	3 cut(s)	4114, 4564, 5542
BcnI	CCSGG	4 cut(s)	133, 514, 530, 2936
BcoDI	GTCTC	9 cut(s)	1229, 2039, 2437, 3312, 3611, 4169, 4692, 5302, 5312
BcuI	ACTAGT	1 cut(s)	4475
BfmI	CTRYAG	7 cut(s)	2095, 2427, 2447, 2838, 3248, 4041, 5778
BfoI	RGCGCY	4 cut(s)	217, 834, 2110, 2292
BfrI	CTTAAG	3 cut(s)	1097, 3425, 4964
BfuAI	ACCTGC	6 cut(s)	128, 2440, 3001, 3181, 3467, 4048
BglI	GCCNNNNNGGC	2 cut(s)	295, 6067
BglII	AGATCT	3 cut(s)	925, 4711, 6553
BlpI	GCTNAGC	3 cut(s)	1011, 2355, 4217
BmcAI	AGTACT	2 cut(s)	2519, 4806
Bme18I	GGWCC	8 cut(s)	171, 224, 1282, 1672, 1988, 2048, 5162, 6314
BmeT110I	CYCGRG	7 cut(s)	293, 755, 4607, 5333, 5891, 6194, 6275
BmrI	ACTGGG	2 cut(s)	5499, 6240
BmtI	GCTAGC	1 cut(s)	2696
BmuI	ACTGGG	2 cut(s)	5499, 6240
BoxI	GACNNNNGTC	1 cut(s)	6384
BpiI	GAAGAC	5 cut(s)	1510, 3405, 5180, 5639, 6528
BplI	GAGNNNNNCTC	4 cut(s)	1298, 1330, 5023, 5055
BpmI	CTGGAG	7 cut(s)	1710, 2733, 3650, 3873, 4080, 6030, 6032
Bpu10I	CCTNAGC	6 cut(s)	3264, 3894, 4539, 4701, 6024, 6578
Bpu1102I	GCTNAGC	3 cut(s)	1011, 2355, 4217
BpuEI	CTTGAG	8 cut(s)	847, 1217, 1619, 1905, 3807, 4358, 4364, 5336
BpuMI	CCSGG	4 cut(s)	133, 514, 530, 2936
BsaAI	YACGTR	4 cut(s)	1085, 2363, 5858, 6206
BsaBI	GATNNNNATC	2 cut(s)	3046, 5886
BsaHI	GRCGYC	2 cut(s)	77, 185
BsaI	GGTCTC	3 cut(s)	3611, 4692, 5312
BsaWI	WCCGGW	6 cut(s)	188, 1480, 3023, 5828, 6316, 6596
BsaXI	ACNNNNNCTCC	6 cut(s)	254, 284, 2791, 2821, 6028, 6058
Bse118I	RCCGGY	5 cut(s)	79, 3023, 3347, 6432, 6596
Bse3DI	GCAATG	8 cut(s)	940, 2030, 3075, 3321, 4195, 4650, 5850, 6306
Bse8I	GATNNNNATC	2 cut(s)	3046, 5886
BseAI	TCCGGA	2 cut(s)	188, 5828
BseJI	GATNNNNATC	2 cut(s)	3046, 5886
BseMI	GCAATG	8 cut(s)	940, 2030, 3075, 3321, 4195, 4650, 5850, 6306
BseRI	GAGGAG	5 cut(s)	435, 3190, 4931, 5768, 6281
BseSI	GKGCMC	4 cut(s)	2599, 3516, 4325, 6099
BseYI	CCCAGC	5 cut(s)	458, 995, 999, 3439, 6422
BsgI	GTGCAG	7 cut(s)	2421, 3696, 3732, 4050, 4314, 5977, 6471
Bsh1236I	CGCG	6 cut(s)	1008, 1440, 2194, 2700, 5067, 5088
BshNI	GGYRCC	6 cut(s)	1753, 3269, 4469, 5359, 5464, 6096
BshTI	ACCGGT	2 cut(s)	3023, 6596
BsiHKAI	GWGCWC	5 cut(s)	14, 1635, 2599, 4608, 4914
BsiHKCI	CYCGRG	7 cut(s)	293, 755, 4607, 5333, 5891, 6194, 6275
BsiWI	CGTACG	1 cut(s)	256
BslFI	GGGAC	6 cut(s)	184, 607, 641, 1104, 1658, 5218
BsmAI	GTCTC	9 cut(s)	1229, 2039, 2437, 3312, 3611, 4169, 4692, 5302, 5312
BsmBI	CGTCTC	1 cut(s)	2039
BsmFI	GGGAC	6 cut(s)	184, 607, 641, 1104, 1658, 5218
BsmI	GAATGC	4 cut(s)	1982, 3241, 3436, 3589
Bso31I	GGTCTC	3 cut(s)	3611, 4692, 5312
BsoBI	CYCGRG	7 cut(s)	293, 755, 4607, 5333, 5891, 6194, 6275
Bsp13I	TCCGGA	2 cut(s)	188, 5828
Bsp1407I	TGTACA	3 cut(s)	768, 876, 2381
Bsp1720I	GCTNAGC	3 cut(s)	1011, 2355, 4217
Bsp19I	CCATGG	6 cut(s)	1331, 1722, 1809, 3596, 4093, 5986
Bsp68I	TCGCGA	1 cut(s)	2700
BspEI	TCCGGA	2 cut(s)	188, 5828
BspFNI	CGCG	6 cut(s)	1008, 1440, 2194, 2700, 5067, 5088
BspHI	TCATGA	5 cut(s)	1657, 2908, 3379, 3418, 5607
BspMAI	CTGCAG	3 cut(s)	2451, 3252, 4045
BspMI	ACCTGC	6 cut(s)	128, 2440, 3001, 3181, 3467, 4048
BspOI	GCTAGC	1 cut(s)	2696
BspQI	GCTCTTC	2 cut(s)	2013, 4164
BspT107I	GGYRCC	6 cut(s)	1753, 3269, 4469, 5359, 5464, 6096
BspTI	CTTAAG	3 cut(s)	1097, 3425, 4964
BspTNI	GGTCTC	3 cut(s)	3611, 4692, 5312
BsrBI	CCGCTC	1 cut(s)	406
BsrDI	GCAATG	8 cut(s)	940, 2030, 3075, 3321, 4195, 4650, 5850, 6306
BsrFI	RCCGGY	5 cut(s)	79, 3023, 3347, 6432, 6596
BsrGI	TGTACA	3 cut(s)	768, 876, 2381
BssAI	RCCGGY	5 cut(s)	79, 3023, 3347, 6432, 6596
BssNI	GRCGYC	2 cut(s)	77, 185
BssSI	CACGAG	5 cut(s)	325, 2717, 4223, 4913, 6072
Bst2BI	CACGAG	5 cut(s)	325, 2717, 4223, 4913, 6072
Bst6I	CTCTTC	8 cut(s)	577, 2013, 3688, 3723, 4164, 4752, 4935, 5024
BstACI	GRCGYC	2 cut(s)	77, 185
BstAFI	CTTAAG	3 cut(s)	1097, 3425, 4964
BstAPI	GCANNNNNTGC	3 cut(s)	614, 5199, 5964
BstAUI	TGTACA	3 cut(s)	768, 876, 2381
BstBAI	YACGTR	4 cut(s)	1085, 2363, 5858, 6206
BstDSI	CCRYGG	7 cut(s)	1331, 1722, 1741, 1809, 3596, 4093, 5986
BstEII	GGTNACC	1 cut(s)	499
BstENI	CCTNNNNNAGG	4 cut(s)	1591, 2173, 3282, 5811
BstFNI	CGCG	6 cut(s)	1008, 1440, 2194, 2700, 5067, 5088
BstH2I	RGCGCY	4 cut(s)	217, 834, 2110, 2292
BstMAI	GTCTC	9 cut(s)	1229, 2039, 2437, 3312, 3611, 4169, 4692, 5302, 5312
BstNSI	RCATGY	3 cut(s)	268, 3804, 3869
BstPAI	GACNNNNGTC	1 cut(s)	6384
BstPI	GGTNACC	1 cut(s)	499
BstSFI	CTRYAG	7 cut(s)	2095, 2427, 2447, 2838, 3248, 4041, 5778
BstSLI	GKGCMC	4 cut(s)	2599, 3516, 4325, 6099
BstUI	CGCG	6 cut(s)	1008, 1440, 2194, 2700, 5067, 5088
BstV2I	GAAGAC	5 cut(s)	1510, 3405, 5180, 5639, 6528
BstXI	CCANNNNNNTGG	1 cut(s)	3674
BtgI	CCRYGG	7 cut(s)	1331, 1722, 1741, 1809, 3596, 4093, 5986
BtgZI	GCGATG	2 cut(s)	3799, 5048
BtsI	GCAGTG	3 cut(s)	2771, 2772, 3744
BtuMI	TCGCGA	1 cut(s)	2700
BveI	ACCTGC	6 cut(s)	128, 2440, 3001, 3181, 3467, 4048
CaiI	CAGNNNCTG	2 cut(s)	704, 5573
CciI	TCATGA	5 cut(s)	1657, 2908, 3379, 3418, 5607
Cfr10I	RCCGGY	5 cut(s)	79, 3023, 3347, 6432, 6596
CseI	GACGC	5 cut(s)	85, 174, 421, 1377, 1446
CsiI	ACCWGGT	1 cut(s)	1408
CspAI	ACCGGT	2 cut(s)	3023, 6596
CspCI	CAANNNNNGTGG	6 cut(s)	2125, 2160, 4267, 4302, 6384, 6419
DraI	TTTAAA	3 cut(s)	1134, 2953, 4989
DraIII	CACNNNGTG	4 cut(s)	4228, 5366, 5569, 6616
DrdI	GACNNNNNNGTC	1 cut(s)	739
DseDI	GACNNNNNNGTC	1 cut(s)	739
EaeI	YGGCCR	7 cut(s)	1030, 1449, 3072, 3652, 3676, 4794, 6059
Eam1104I	CTCTTC	8 cut(s)	577, 2013, 3688, 3723, 4164, 4752, 4935, 5024
EarI	CTCTTC	8 cut(s)	577, 2013, 3688, 3723, 4164, 4752, 4935, 5024
EciI	GGCGGA	3 cut(s)	262, 5958, 6392
Ecl136II	GAGCTC	1 cut(s)	1633
Eco147I	AGGCCT	1 cut(s)	5035
Eco24I	GRGCYC	3 cut(s)	1635, 5607, 6046
Eco31I	GGTCTC	3 cut(s)	3611, 4692, 5312
Eco32I	GATATC	2 cut(s)	2571, 3385
Eco47I	GGWCC	8 cut(s)	171, 224, 1282, 1672, 1988, 2048, 5162, 6314
Eco53kI	GAGCTC	1 cut(s)	1633
Eco57I	CTGAAG	7 cut(s)	911, 926, 1251, 1275, 2208, 3724, 5040
Eco72I	CACGTG	2 cut(s)	1085, 2363
Eco88I	CYCGRG	7 cut(s)	293, 755, 4607, 5333, 5891, 6194, 6275
Eco91I	GGTNACC	1 cut(s)	499
EcoICRI	GAGCTC	1 cut(s)	1633
EcoNI	CCTNNNNNAGG	4 cut(s)	1591, 2173, 3282, 5811
EcoO109I	RGGNCCY	4 cut(s)	171, 1672, 2048, 3558
EcoO65I	GGTNACC	1 cut(s)	499
EcoRI	GAATTC	1 cut(s)	3337
EcoRV	GATATC	2 cut(s)	2571, 3385
EcoT22I	ATGCAT	1 cut(s)	1982
EcoT38I	GRGCYC	3 cut(s)	1635, 5607, 6046
Esp3I	CGTCTC	1 cut(s)	2039
FaqI	GGGAC	6 cut(s)	184, 607, 641, 1104, 1658, 5218
FauI	CCCGC	3 cut(s)	89, 5195, 5655
FauNDI	CATATG	1 cut(s)	2712
FbaI	TGATCA	3 cut(s)	4114, 4564, 5542
FriOI	GRGCYC	3 cut(s)	1635, 5607, 6046
FspI	TGCGCA	1 cut(s)	3408
GsaI	CCCAGC	5 cut(s)	462, 999, 1003, 3443, 6426
GsuI	CTGGAG	7 cut(s)	1710, 2733, 3650, 3873, 4080, 6030, 6032
HaeII	RGCGCY	4 cut(s)	217, 834, 2110, 2292
HgaI	GACGC	5 cut(s)	85, 174, 421, 1377, 1446
Hin1I	GRCGYC	2 cut(s)	77, 185
HincII	GTYRAC	5 cut(s)	1069, 5668, 5692, 5704, 6496
HindII	GTYRAC	5 cut(s)	1069, 5668, 5692, 5704, 6496
HindIII	AAGCTT	5 cut(s)	1764, 1886, 2610, 2846, 4004
HpaI	GTTAAC	3 cut(s)	1069, 5668, 5704
HpyCH4IV	ACGT	9 cut(s)	1084, 1422, 1548, 2143, 2362, 3955, 5857, 6205, 6352
HpySE526I	ACGT	9 cut(s)	1084, 1422, 1548, 2143, 2362, 3955, 5857, 6205, 6352
Hsp92I	GRCGYC	2 cut(s)	77, 185
KflI	GGGWCCC	2 cut(s)	171, 1672
Kpn2I	TCCGGA	2 cut(s)	188, 5828
KroI	GCCGGC	1 cut(s)	6432
KroNI	GCCGGC	1 cut(s)	6434
Ksp22I	TGATCA	3 cut(s)	4114, 4564, 5542
KspAI	GTTAAC	3 cut(s)	1069, 5668, 5704
LguI	GCTCTTC	2 cut(s)	2013, 4164
MabI	ACCWGGT	1 cut(s)	1408
MaeII	ACGT	9 cut(s)	1084, 1422, 1548, 2143, 2362, 3955, 5857, 6205, 6352
MbiI	CCGCTC	1 cut(s)	406
MfeI	CAATTG	3 cut(s)	390, 1938, 3393
MlsI	TGGCCA	6 cut(s)	1032, 1451, 3074, 3678, 4796, 6061
MluI	ACGCGT	1 cut(s)	1438
MluNI	TGGCCA	6 cut(s)	1032, 1451, 3074, 3678, 4796, 6061
MmeI	TCCRAC	7 cut(s)	243, 381, 529, 1429, 3463, 3931, 4186
Mox20I	TGGCCA	6 cut(s)	1032, 1451, 3074, 3678, 4796, 6061
Mph1103I	ATGCAT	1 cut(s)	1982
MroI	TCCGGA	2 cut(s)	188, 5828
MroNI	GCCGGC	1 cut(s)	6432
MroXI	GAANNNNTTC	4 cut(s)	161, 164, 4737, 5745
MscI	TGGCCA	6 cut(s)	1032, 1451, 3074, 3678, 4796, 6061
MslI	CAYNNNNRTG	9 cut(s)	1038, 2330, 2823, 2979, 3080, 3776, 4318, 4572, 6617
Msp20I	TGGCCA	6 cut(s)	1032, 1451, 3074, 3678, 4796, 6061
MspA1I	CMGCKG	6 cut(s)	889, 904, 999, 5525, 6422, 6485
MspCI	CTTAAG	3 cut(s)	1097, 3425, 4964
MunI	CAATTG	3 cut(s)	390, 1938, 3393
Mva1269I	GAATGC	4 cut(s)	1982, 3241, 3436, 3589
MvnI	CGCG	6 cut(s)	1008, 1440, 2194, 2700, 5067, 5088
NaeI	GCCGGC	1 cut(s)	6434
NciI	CCSGG	4 cut(s)	133, 514, 530, 2936
NcoI	CCATGG	6 cut(s)	1331, 1722, 1809, 3596, 4093, 5986
NdeI	CATATG	1 cut(s)	2712
NgoMIV	GCCGGC	1 cut(s)	6432
NheI	GCTAGC	1 cut(s)	2692
NmeAIII	GCCGAG	3 cut(s)	67, 72, 2785
NruI	TCGCGA	1 cut(s)	2700
NsbI	TGCGCA	1 cut(s)	3408
NsiI	ATGCAT	1 cut(s)	1982
NspI	RCATGY	3 cut(s)	268, 3804, 3869
OliI	CACNNNNGTG	2 cut(s)	1038, 2330
PaeR7I	CTCGAG	4 cut(s)	755, 5891, 6194, 6275
PagI	TCATGA	5 cut(s)	1657, 2908, 3379, 3418, 5607
PaqCI	CACCTGC	2 cut(s)	2440, 3467
PceI	AGGCCT	1 cut(s)	5035
PciI	ACATGT	2 cut(s)	3800, 3865
PciSI	GCTCTTC	2 cut(s)	2013, 4164
PcsI	WCGNNNNNNNCGW	2 cut(s)	300, 6079
PctI	GAATGC	4 cut(s)	1982, 3241, 3436, 3589
PdiI	GCCGGC	1 cut(s)	6434
PdmI	GAANNNNTTC	4 cut(s)	161, 164, 4737, 5745
Pfl23II	CGTACG	1 cut(s)	256
PflFI	GACNNNGTC	4 cut(s)	1410, 1670, 5179, 6287
PflMI	CCANNNNNTGG	3 cut(s)	1337, 2783, 4099
PfoI	TCCNGGA	4 cut(s)	131, 2749, 3298, 6046
PinAI	ACCGGT	2 cut(s)	3023, 6596
PmaCI	CACGTG	2 cut(s)	1085, 2363
PmlI	CACGTG	2 cut(s)	1085, 2363
Ppu21I	YACGTR	4 cut(s)	1085, 2363, 5858, 6206
PpuMI	RGGWCCY	3 cut(s)	171, 1672, 2048
PscI	ACATGT	2 cut(s)	3800, 3865
PshAI	GACNNNNGTC	1 cut(s)	6384
PshBI	ATTAAT	2 cut(s)	3413, 3873
Psp124BI	GAGCTC	1 cut(s)	1635
Psp5II	RGGWCCY	3 cut(s)	171, 1672, 2048
PspCI	CACGTG	2 cut(s)	1085, 2363
PspEI	GGTNACC	1 cut(s)	499
PspFI	CCCAGC	5 cut(s)	458, 995, 999, 3439, 6422
PspLI	CGTACG	1 cut(s)	256
PspPPI	RGGWCCY	3 cut(s)	171, 1672, 2048
PspXI	VCTCGAGB	3 cut(s)	755, 6194, 6275
PsrI	GAACNNNNNNTAC	2 cut(s)	1638, 1670
PstI	CTGCAG	3 cut(s)	2451, 3252, 4045
PstNI	CAGNNNCTG	2 cut(s)	704, 5573
PsyI	GACNNNGTC	4 cut(s)	1410, 1670, 5179, 6287
PvuII	CAGCTG	4 cut(s)	889, 904, 999, 5525
RruI	TCGCGA	1 cut(s)	2700
RseI	CAYNNNNRTG	9 cut(s)	1038, 2330, 2823, 2979, 3080, 3776, 4318, 4572, 6617
SacI	GAGCTC	1 cut(s)	1635
SapI	GCTCTTC	2 cut(s)	2013, 4164
ScaI	AGTACT	2 cut(s)	2519, 4806
SexAI	ACCWGGT	1 cut(s)	1408
SfcI	CTRYAG	7 cut(s)	2095, 2427, 2447, 2838, 3248, 4041, 5778
SfiI	GGCCNNNNNGGCC	1 cut(s)	6067
Sfr274I	CTCGAG	4 cut(s)	755, 5891, 6194, 6275
SinI	GGWCC	8 cut(s)	171, 224, 1282, 1672, 1988, 2048, 5162, 6314
SlaI	CTCGAG	4 cut(s)	755, 5891, 6194, 6275
SmiMI	CAYNNNNRTG	9 cut(s)	1038, 2330, 2823, 2979, 3080, 3776, 4318, 4572, 6617
SpeI	ACTAGT	1 cut(s)	4475
SseBI	AGGCCT	1 cut(s)	5035
SspI	AATATT	5 cut(s)	1583, 4015, 4073, 4441, 5713
SstI	GAGCTC	1 cut(s)	1635
StuI	AGGCCT	1 cut(s)	5035
TaiI	ACGT	9 cut(s)	1087, 1425, 1551, 2146, 2365, 3958, 5860, 6208, 6355
TaqII	GACCGA	1 cut(s)	4674
TauI	GCSGC	5 cut(s)	110, 372, 4979, 6422, 6485
TspGWI	ACGGA	7 cut(s)	13, 398, 1668, 2472, 3178, 3920, 6071
Tth111I	GACNNNGTC	4 cut(s)	1410, 1670, 5179, 6287
Van91I	CCANNNNNTGG	3 cut(s)	1337, 2783, 4099
Vha464I	CTTAAG	3 cut(s)	1097, 3425, 4964
VneI	GTGCAC	1 cut(s)	2595
VpaK11BI	GGWCC	8 cut(s)	171, 224, 1282, 1672, 1988, 2048, 5162, 6314
VspI	ATTAAT	2 cut(s)	3413, 3873
XagI	CCTNNNNNAGG	4 cut(s)	1591, 2173, 3282, 5811
XapI	RAATTY	4 cut(s)	2948, 3337, 4280, 5849
XbaI	TCTAGA	1 cut(s)	3563
XceI	RCATGY	3 cut(s)	268, 3804, 3869
XcmI	CCANNNNNNNNNTGG	6 cut(s)	1729, 2068, 2503, 3686, 4804, 6054
XhoI	CTCGAG	4 cut(s)	755, 5891, 6194, 6275
XmnI	GAANNNNTTC	4 cut(s)	161, 164, 4737, 5745
ZrmI	AGTACT	2 cut(s)	2519, 4806
Zsp2I	ATGCAT	1 cut(s)	1982

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.