Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0008640

Physical Location & Seq

Genomic Coordinates Forward (+)

66938133 .. 66947607

Gene Structure 9475 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG450100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6615 bp

ATGTTGGCGGCGAGTTCCGGCGGTGGTGGTTCCGTTCTCCAACTCCGGACCAAACCTTCCGTCCTCGCTTCACCTCAGCTCAATGCCTCGCCGATAGCGAGGCTAAGCACCGGAGCCACAGCTCGCTCTGGTTCCAAAGCTATTGCGAAGAAGTTCTTCGGGACGAGATTGAGGGCCGCCGGATCCGAGAGGCTGCACTTATGGCGATCCGACGGTCCCGGCCGCTCGCCGAAGCTGCGGGTGGTGGTCCGGAACATGCTCTCCGCCGTGCCGGAGAAGCCGCTCGGGCTATACGACCCGGCGATGGATAAGGACTCCTGCGGTGTCGGCTTCGTGGCGGAATTGTCCGGTGAAAGCAGCCGTAAAACGATTACGGATGCTCTGGAAATGTTGGTGCGGATGACACATAGAGGAGCTTGTGGATGTGAAACAAACACTGGTGATGGAGCTGGTGTTCTTGTGGCGATTCCTCACGATTTCTACAAGGAGGCTGCCAAAGATAATGGGTTTGAGCTTCCACCGCTGGGTGAGTATGCTGTTGGTATGCTCTACTTGCCTACATCGGAGAGTCGTAGAGAGGAAAGCAAAAATGTGTTTACAAAGGTTGCCGAGTCTCTTGGCCATACTGTTCTTGGGTGGCGATCAGTTCCCACTGATAACTCAGATTTGGGGAATTCTGCTCTCCAGACAGAACCTGTTATCGAGCAAGTGTTCCTTACTTCAACTCCGAGGTCGAAAGTTGATCTTGAGCGCCAGATGTACATACTTAGAAGGGTTTCAATGGTTGCCATCCGTGCTGCATTAAACCTTCAACATGGTGGTGCTAAAGATTTTTACATATGTTCACTTTCCTCGAGGACGATTGTTTACAAGGGTCAGCTTAAACCTGAACAGCTGAAGGGTTACTATTATGCAGATCTTGGCAATGAAAGGTTTACGAGCTACATGGCCCTGGTACATTCTCGATTCTCAACAAATACTTTCCCCAGCTGGGACCGTGCTCAGCCCATGCGTGTCATTGGCCACAATGGAGAAATCAACACCCTTAGAGGAAATGTTAACTGGATGAAGGCACGTGAGGGACTGCTCAAGTGTACTGAACTTGGTTTATCAAAAAATGAGTTAAAGAAGCTGTTACCAATTGTGGATGCCAGTTCATCCGATTCAGGAGCTTTTGATGGCGTCCTTGAGCTTCTAGTTCGAGCTGGCAGAAGCCTCCCTGAAGCTATTATGATGATGATTCCTGAAGCATGGCAAAATGACAAGAACATGGATCCTGATAGGAGAGCACTGTATGAATATTTCTCATCTCTCATGGAGCCATGGGATGGGCCAGCTCTAATATCATTTACTGATGGACGCTATCTGGGAGCTACATTGGATCGGAATGGACTCCGACCGGGTCGCTTTTACGTCACCCATAGTGGACGAGTTATTATGGCCAGTGAAGTTGGGGTTGTAGACGTACCTCCAGAAGATGTCTGCAGGAAAGGAAGACTTAACCCTGGAATGATGCTTTTGGTGGACTTTGAGAATCATATAGTGGTAGATGATGAAGCCTTGAAGCAGCAATATTCACTGGCAAGGCCGTATGGGGAGTGGCTTAAAAGGCAAAAGATTGAGCTCAAGGACATAGTTGATTCTGTTAATGAATCTGACAGGGTCCCTCCATCCATAGCTGGAGTTGTTCCTGCATCTACTGATGATGAGAACATGGAAAACATGGGAGTCCATGGTTTACTGGCACCCCTGAAGGCTTTTGGTTACACAGTGGAAGCCTTGGAAATGTTGTTGCTTCCTATGGCAAAAGATGGCGTAGAGGCTCTTGGGTCGATGGGAAATGATACCCCCTTGGCTGTTATGTCCAATAGAGAAAAGCTTACATTTGAGTATTTCAAGCAAATGTTCGCCCAAGTAACAAACCCTCCAATTGATCCAATTCGAGAAAAGGTAGTCACGTCTATGGAATGTATGATCGGTCCAGAAGGTGATCTAACAGAAACCACTGAAGAACAATGCCATCGTCTCTCACTCAAAGGTCCTCTTTTAAGCATTGAAGAAATGGAGGCGATCAAAAAAATGAATTACAGAGGTTGGCGCTGTAAGGTTCTTGACATAACTTACTCCAAGGAACGTGGTAGAAAGGGATTGGAGGAGACCTTGGATAGGATCTGTGCTGAAGCACGTGAGGCAATTAAAAAGGGATATACCACACTGGTGCTTTCAGACAGAGCCTTCTCCCCAAAACGTGTTGCTGTAAGCTCACTCTTGGCTGTTGGTGCTGTTCATCAACATCTAGTTAAAAATCTTGAGCGCACGCGAGTAGGGTTGATAATTGAATCTGCTGAGCCGCGTGAAGTACATCATTTCTGTACGTTAGTTGGATTCGGTGCAGATGCCATTTGCCCCTACCTGGCTGTAGAGGCTATTTGGAGACTGCAGGTTGATGGAAAGATCCCACCAAAATCTAATGGTACAATATACTCCAAGGAAGAACTGGTTAAAAAGTACTTCAAAGCAAGCAACTATGGAATGCAGAAAGTTCTTGCCAAGATGGGGATATCTACTTTGGCCTCTTACAAAGGTGCACAGATTTTTGAAGCTTTGGGTCTTTCGTCTGAAGTAATTGAGAGATGCTTTGCAGGAACTCCTAGTAGAGTTGAGGGTGCAACATTTGAGATGCTTGCTCGTGATGGACTTCATCTGCATGATTTGGCATTTCCCTCTCGGGCTTTTCCTCCTGGTAGTGCAGAAGCTGTGGCACTACCAAATCCTGGGGATTATCATTGGAGAAAAGGTGGTGAAGTTCACCTAAATGATCCTTTTGCTATTTCCAAGCTTCAGGAGGCTGCCAGAACTAACAGTGTGGCGGCATATAAAGAATACTCCAAGCTCATTCATCAATTAAATAAAGCCTGCAATCTCCGTGGTCTTCTGAAATTTAAAAACACAGAGCAGCAGATTCACTTGGATGAAGTGGAACCTGCCAGTGAGATTGTTAAACGGTTCTGTACTGGGGCAATGAGTTATGGATCAATATCATTGGAGGCACACAGTACACTTGCCATTGCTATGAACAGAATGGGAGGAAAGTCGAATACAGGTGAGGGAGGTGAGCAGCCCTCTCGTATGGAGCCTCTTCCTGATGGTTCAAGGAATCCAAAGAGGAGTGCAATTAAGCAGGTTGCAAGTGGGAGATTTGGAGTTTCAAGTTACTATCTTACAAATGCTGATGAACTCCAAATAAAAATGGCTCAGGGGGCCAAGCCTGGTGAAGGAGGTGAGCTTCCTGGTCACAAGGTTATTGGAGACATTGCTGTTACCAGGAATTCCACTGCTGGTGTGGGACTTATCAGCCCACCTCCCCATCATGATATCTACTCAATTGAAGACCTTGCTCAATTAATTCATGATCTTAAGAATGCTAACCCAGGGGCTCGAATCAGCGTCAAGTTAGTATCTGAAGCTGGTGTAGGAGTAGTTGCTAGTGGAGTTGTGAAGGGGCATGCAGACCATGTTTTAATTGCCGGACATGATGGAGGCACTGGGGCCTCTAGATGGACTGGCATTAAGAATGCTGGGCTTCCATGGGAACTTGGCTTGGCTGAGACCCATCAAACCTTAGTTGCTAATGATCTTCGTGGCCGCACAGTTCTCCAGACAGATGGCCAACTAAAAACTGGAAGAGATGTGGCCATAGCCGCACTTCTCGGTGCAGAAGAGTTTGGGTTCAGCACAGCACCCCTCATAACCCTTGGCTGCATTATGATGCGGAAGTGCCACAAGAACACCTGTCCTGTCGGAATTGCTACCCAAGATCCAGTACTTCGTGAGAAGTTTGCTGGAGAACCTGAACATGTTATCAACTTTTTCTTCATGGTAGCTGAGGAGGTGAGAGAGATAATGTCACAACTTGGTTTTCGAACTTTAAATGAAATGGTTGGTCGTTCAGATATGCTTGAAGTTGATAAAGAAGTTACTAAAGACAATGAGAAGCTGAACAACATTGATCTCTCCCTGTTACTTAGACCTGCTGCAGACATTCGGCCTGAAGCTGCACAATATTGTGTTCAGAAACAGGACCATGGGTTGGACATGGCTCTTGACCACAAACTTATTTCGCTGTCCAGTTCTGCTATAGAGAAGGCTCTCCCTGCATACTTTGAAACACCAATTTGCAATGTGAATCGTGCTGTTGGAACAATGCTTAGTCATGAGGTGACCAAACGTTACAACAGGGAAGGGCTTCCAGCAGACACCATCCATATCAAATTCAATGGAAGTGCAGGCCAGAGCCTTGGAGCTTTCCTCTGCCCTGGCATCACGCTTGAGCTTGAAGGCGACAGCAATGATTACGTCGGTAAAGGATTGTCTGGTGGCAAGATCATAGTTTATCCCCCAAAGGGAAGCAAGTTTGACCCGAAAGAAAACATTGTCATTGGTAATGTAGCTCTTTATGGTGCCACTAGTGGGGAGGCATATTTCAATGGAATGGCAGCAGAAAGATTCTGTGTTCGCAATTCGGGGGCTAGGGCAGTAGTTGAAGGTGTTGGTGATCATGGCTGTGAGTACATGACAGGTGGCACTGTTGTTGTGCTTGGGAAAACTGGCAGGAACTTTGCTGCTGGTATGAGTGGTGGCATAGCCTACGTTCTTGATGTGGACGGAAAATTTTTGTCCCGCTGCAATCCTGAGCTTGTAGATCTGGATAAAGTTGAAGAAGAAGATAGTACGACTCTTAAAATGTTGATACAGCAGCATCAACGTCATACAAAAAGCTTGCTGGCCAGTGAAGTACTTGCTGATTTTGAGAATCTTTTGCCTAAATTCATCAAGGTTATCCCCAGAGAGTACAAGCGTGCACTTGCAAACTTGAGGGAGGAGGCCTCCAAAGCTGATGAAGAAGCTCAGGAGCAAGAGGAGGAGGTGGAAATAAAGGAGAAAGATGCATTTGAAGAACTTAAGAAGATGGCATCTGCATCTTTAAATGGGACATCCAATCAGGTAGAAGATGCTGAAACATTGAAGAGGCCCAGTGAGGTCACCAAAGCTGTTAAACACCGGGGTTTCATTTCTTATGAGCGTGAGGGCGTTCAATACAGGGATCCCAAAGTTCGGATGAATGACTGGAACGAAGTTATGGAGGAAACTAAACCTGGTCCACTTGTGAATACGCAGTCAGCCCGTTGCATGGACTGTGGCACTCCTTTCTGCCATCAGGAGAACACAGGGTGTCCTCTTGGTAACAAAATACCCGAGTTCAATGAATTGGTGTACCAGAATAGGTGGCATGAAGCATTAGAGAGACTCCTTGAGACAAATAACTTCCCTGAGTTCACTGGTCGAGTTTGTCCGGCACCTTGTGAAGGTTCATGCGTTCTGGGTATTATTGAGAATCCTGTCTCTATCAAAAGCATTGAATGTGCCATCATAGACAAGGCTTTTGAGGAAGGTTGGATGGTGCCACGGCCTCCACTCAAGAGAACTGGGAAAAAGGTTGCCATTGTCGGAAGTGGGCCTGCTGGGCTAGCTGCTGCTGATCAACTAAACAGAATAGGTCATACAGTGACAGTTTACGAGCGTGCTGATAGAATTGGGGGGCTTATGATGTATGGAGTGCCTAATATGAAGGCTGACAAAGTTGATATAGTTCAACGGCGGGTTAACCTTATGGCCGAGGAAGGTGTCAACTTTGTGGTCAATGCTAATGTTGGAAATGATCCTTCCTACTCGTTTGATCTCCTTCGGGAGGAGAACAATGCGATTATTTTAGCAGTAGGCGCAACAAAACCAAGGGACCTTCCTGTACCTGGGCGAGAGCTGTCTGGAGTACATTTCGCTATGGAGTTTCTTCATGCAAACACCAAAAGCTTGCTTGATAGCAATCTCGAGAATGGTAACTATATTTCTGCCAAGGGTAAGAAGGTTGTGGTCATTGGTGGAGGTGACACTGGCACCGATTGTATTGGAACCTCAGTTCGCCATGGCTGTACTAACATTGTAAATTTGGAGCTTCTCCCTGAGCCACCACGAACAAGGGCACCAGGAAACCCTTGGCCACAGTGGCCTCGTATATTCCGAGTAGATTATGGGCATGCAGAAGTTGCAGCCAAGTTTGGCAAAGATCCAAGAACTTATGAGGTGCTGACTAAGAGGTTTGTGGGAGATGAGAATGGGGTTGTGAAGGGACTTGAGGTAGTACGTGTCAAATGGGAGAAAGATGCTACTGGCAAGTTCCAATTTAAGGAAATCGAGGGCTCTGAGGAGATAATTGAGGCCGATCTCGTCCTTCTAGCAATGGGGTTCCTTGGACCTGAGGCGGCGATTGCAGAGAAGTTGGGCTTGGAGTGTGACAATCGCTCGAACTTCAAGGCAGATTATGGTCGGTTCTCAACCAATGTGGATGGGGTCTTCGCTGCTGGGGATTGCCGGCGTGGTCAATCCCTGGTGGTGTGGGCAATCTCAGAGGGACGGCAAGCAGCTGCACAGGTTGACAACTACCTCTGCAAGGAAGAAGAAGAATACACAAACAGCAACGCGAGTCCTGAATCCGATCTCTTGAAGCGGCATCAGGAAATCAGCAAGAGCCATAAGACAGTTGCAAGCACACCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015930 GO:0016040 GO:0016053 GO:0016491 GO:0016638 GO:0016639 GO:0019676 GO:0019740 GO:0019752 GO:0032502 GO:0040007 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045181 GO:0046394 GO:0046686 GO:0048589 GO:0050896 GO:0055114 GO:0060359 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00250 ko00910 ko01100 ko01110 ko01120 ko01130 ko01230 map00250 map00910 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

Fer4_20 GATase_2 GXGXG Glu_syn_central Glu_synthase Pyr_redox_2

Protein Analysis

2204

Amino Acids

241.39

Weight (kDa)

6.12

Isoelectric Point (pI)

36.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	107 - 534	1.7e-184	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	584 - 872	1.6e-112	Glutamate synthase central domain
Glu_synthase	PF01645	939 - 1307	8e-150	Conserved region in glutamate synthase
GXGXG	PF01493	1391 - 1577	1.7e-89	GXGXG motif
Fer4_20	PF14691	1707 - 1818	7.9e-22	Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
Pyr_redox_2	PF07992	1831 - 2157	5.4e-28	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	1835 - 1870	1.9e-08	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0008640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53460 AT5G53460 AT5G53460
fragaria_vesca	FvH4_7g31770 FvH4_7g31770 FvH4_7g31770
malus_domestica	MD01G1219500.v1.1 MD07G1290500.v1.1
prunus_persica	Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1
pyrus_communis	pycom01g22930 pycom07g26320
rosa_chinensis	RchiOBHm_Chr1g0381001
rosa_laevigata	RLG00000026253
rosa_multiflora	Rmu_ssc0000032.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278870
rosa_rugosa	Rorug01G0426400
rosa_samantha	Rh1AG450100 Rh1BG406100 Rh1CG420100 Rh1DG436600
rosa_wichuraiana	Rw1G039220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	853
Acc36I	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
AccB1I	GGYRCC	6 cut(s)	1744, 4460, 5356, 5461, 5954, 6040
AccB7I	CCANNNNNTGG	3 cut(s)	1328, 2774, 4090
AccBSI	CCGCTC	2 cut(s)	225, 283
AccI	GTMKAC	1 cut(s)	1461
AccII	CGCG	3 cut(s)	2320, 2353, 6539
AccIII	TCCGGA	2 cut(s)	45, 249
AclI	AACGTT	1 cut(s)	4228
AcsI	RAATTY	7 cut(s)	673, 2939, 3328, 4271, 4670, 4826, 6004
AcvI	CACGTG	2 cut(s)	1076, 2186
AcyI	GRCGYC	1 cut(s)	1182
AdeI	CACNNNGTG	2 cut(s)	5232, 5363
AflII	CTTAAG	2 cut(s)	3416, 4961
AflIII	ACRYGT	3 cut(s)	2248, 3856, 6202
AhlI	ACTAGT	1 cut(s)	4466
AjiI	CACGTC	1 cut(s)	1959
AjuI	GAANNNNNNNTTGG	2 cut(s)	6326, 6358
AleI	CACNNNNGTG	1 cut(s)	2216
AloI	GAACNNNNNNTCC	6 cut(s)	245, 277, 438, 470, 5961, 5993
Alw21I	GWGCWC	5 cut(s)	1003, 1291, 1626, 2590, 4864
Alw26I	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5299, 5309, 5407
Alw44I	GTGCAC	2 cut(s)	2586, 4860
AlwNI	CAGNNNCTG	2 cut(s)	695, 2756
Ama87I	CYCGRG	5 cut(s)	284, 853, 2727, 5255, 5888
Aor13HI	TCCGGA	2 cut(s)	45, 249
ApaLI	GTGCAC	2 cut(s)	2586, 4860
ApoI	RAATTY	7 cut(s)	673, 2939, 3328, 4271, 4670, 4826, 6004
ArsI	GACNNNNNNTTYG	4 cut(s)	45, 77, 5426, 5458
AseI	ATTAAT	1 cut(s)	3404
Asp700I	GAANNNNTTC	4 cut(s)	152, 155, 775, 4245
AspLEI	GCGC	4 cut(s)	753, 2100, 2316, 5783
AsuC2I	CCSGG	4 cut(s)	219, 299, 1401, 5063
AsuII	TTCGAA	1 cut(s)	3922
AsuNHI	GCTAGC	1 cut(s)	5527
AvaI	CYCGRG	5 cut(s)	284, 853, 2727, 5255, 5888
AxyI	CCTNAGG	1 cut(s)	6315
BaeGI	GKGCMC	3 cut(s)	2590, 4864, 6043
BalI	TGGCCA	7 cut(s)	621, 1023, 1442, 3669, 3695, 4787, 6058
BamHI	GGATCC	3 cut(s)	182, 1273, 5104
BanI	GGYRCC	6 cut(s)	1744, 4460, 5356, 5461, 5954, 6040
BanII	GRGCYC	3 cut(s)	1626, 3439, 6260
BauI	CACGAG	1 cut(s)	2688
BbrPI	CACGTG	2 cut(s)	1076, 2186
BbsI	GAAGAC	4 cut(s)	1501, 2924, 3396, 6403
Bbv12I	GWGCWC	5 cut(s)	1003, 1291, 1626, 2590, 4864
BbvCI	CCTCAGC	2 cut(s)	75, 3885
BceAI	ACGGC	6 cut(s)	251, 345, 1573, 5483, 5672, 6488
BcgI	CGANNNNNNTGC	2 cut(s)	3107, 3141
BclI	TGATCA	2 cut(s)	4555, 5539
BcnI	CCSGG	4 cut(s)	219, 299, 1401, 5063
BcoDI	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5299, 5309, 5407
BcuI	ACTAGT	1 cut(s)	4466
BfaI	CTAG	9 cut(s)	1196, 2297, 2652, 3486, 3555, 4467, 4530, 5528, 6293
BfmI	CTRYAG	5 cut(s)	1483, 2418, 2438, 4035, 4137
BfoI	RGCGCY	2 cut(s)	754, 2101
BfrI	CTTAAG	2 cut(s)	3416, 4961
BfuAI	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
BglI	GCCNNNNNGGC	3 cut(s)	286, 5524, 6064
BglII	AGATCT	2 cut(s)	916, 4702
BlpI	GCTNAGC	3 cut(s)	104, 1002, 2346
BmcAI	AGTACT	3 cut(s)	2510, 3825, 4797
BmeT110I	CYCGRG	5 cut(s)	284, 853, 2727, 5255, 5888
BmgBI	CACGTC	1 cut(s)	1959
BmrI	ACTGGG	4 cut(s)	3024, 3555, 5028, 5496
BmtI	GCTAGC	1 cut(s)	5531
BmuI	ACTGGG	4 cut(s)	3024, 3555, 5028, 5496
BpiI	GAAGAC	4 cut(s)	1501, 2924, 3396, 6403
BplI	GAGNNNNNCTC	6 cut(s)	3107, 3139, 4293, 4325, 4871, 4903
BpmI	CTGGAG	6 cut(s)	668, 1455, 1701, 3641, 3864, 5847
Bpu10I	CCTNAGC	6 cut(s)	75, 3255, 3885, 4692, 4908, 6021
Bpu1102I	GCTNAGC	3 cut(s)	104, 1002, 2346
Bpu14I	TTCGAA	1 cut(s)	3922
BpuMI	CCSGG	4 cut(s)	219, 299, 1401, 5063
BsaAI	YACGTR	3 cut(s)	1076, 2186, 6203
BsaBI	GATNNNNATC	2 cut(s)	3037, 5883
BsaHI	GRCGYC	1 cut(s)	1182
BsaI	GGTCTC	2 cut(s)	2150, 3602
BsaWI	WCCGGW	4 cut(s)	45, 110, 249, 347
Bse118I	RCCGGY	1 cut(s)	6429
Bse21I	CCTNAGG	1 cut(s)	6315
Bse3DI	GCAATG	7 cut(s)	931, 3027, 3066, 3312, 4186, 4354, 6303
Bse8I	GATNNNNATC	2 cut(s)	3037, 5883
BseAI	TCCGGA	2 cut(s)	45, 249
BseJI	GATNNNNATC	2 cut(s)	3037, 5883
BseMI	GCAATG	7 cut(s)	931, 3027, 3066, 3312, 4186, 4354, 6303
BseRI	GAGGAG	9 cut(s)	426, 2168, 3181, 3902, 4895, 4934, 4937, 5765, 6278
BseSI	GKGCMC	3 cut(s)	2590, 4864, 6043
BseX3I	CGGCCG	1 cut(s)	220
BseYI	CCCAGC	6 cut(s)	523, 986, 990, 3578, 5522, 6419
BsgI	GTGCAG	7 cut(s)	179, 2412, 2769, 3735, 4041, 4305, 6468
Bsh1236I	CGCG	3 cut(s)	2320, 2353, 6539
Bsh1285I	CGRYCG	2 cut(s)	223, 1400
BshNI	GGYRCC	6 cut(s)	1744, 4460, 5356, 5461, 5954, 6040
BsiEI	CGRYCG	2 cut(s)	223, 1400
BsiHKAI	GWGCWC	5 cut(s)	1003, 1291, 1626, 2590, 4864
BsiHKCI	CYCGRG	5 cut(s)	284, 853, 2727, 5255, 5888
BsmAI	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5299, 5309, 5407
BsmBI	CGTCTC	1 cut(s)	2030
BsmI	GAATGC	3 cut(s)	2538, 3427, 3580
Bso31I	GGTCTC	2 cut(s)	2150, 3602
BsoBI	CYCGRG	5 cut(s)	284, 853, 2727, 5255, 5888
Bsp119I	TTCGAA	1 cut(s)	3922
Bsp1286I	GDGCHC	8 cut(s)	1003, 1291, 1626, 2590, 3439, 4864, 6043, 6260
Bsp13I	TCCGGA	2 cut(s)	45, 249
Bsp1407I	TGTACA	1 cut(s)	759
Bsp1720I	GCTNAGC	3 cut(s)	104, 1002, 2346
Bsp19I	CCATGG	5 cut(s)	1322, 1732, 3587, 4084, 5983
BspEI	TCCGGA	2 cut(s)	45, 249
BspFNI	CGCG	3 cut(s)	2320, 2353, 6539
BspHI	TCATGA	3 cut(s)	3370, 3409, 4213
BspMAI	CTGCAG	3 cut(s)	1487, 2442, 4039
BspMI	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
BspOI	GCTAGC	1 cut(s)	5531
BspT104I	TTCGAA	1 cut(s)	3922
BspT107I	GGYRCC	6 cut(s)	1744, 4460, 5356, 5461, 5954, 6040
BspTI	CTTAAG	2 cut(s)	3416, 4961
BspTNI	GGTCTC	2 cut(s)	2150, 3602
BsrBI	CCGCTC	2 cut(s)	225, 283
BsrDI	GCAATG	7 cut(s)	931, 3027, 3066, 3312, 4186, 4354, 6303
BsrFI	RCCGGY	1 cut(s)	6429
BsrGI	TGTACA	1 cut(s)	759
BssAI	RCCGGY	1 cut(s)	6429
BssNI	GRCGYC	1 cut(s)	1182
BssSI	CACGAG	1 cut(s)	2688
Bst2BI	CACGAG	1 cut(s)	2688
Bst6I	CTCTTC	4 cut(s)	3144, 3679, 3714, 5021
BstACI	GRCGYC	1 cut(s)	1182
BstAFI	CTTAAG	2 cut(s)	3416, 4961
BstAPI	GCANNNNNTGC	1 cut(s)	6104
BstAUI	TGTACA	1 cut(s)	759
BstBAI	YACGTR	3 cut(s)	1076, 2186, 6203
BstBI	TTCGAA	1 cut(s)	3922
BstDSI	CCRYGG	7 cut(s)	1322, 1732, 2926, 3587, 4084, 5465, 5983
BstEII	GGTNACC	2 cut(s)	4219, 5041
BstENI	CCTNNNNNAGG	5 cut(s)	2164, 3273, 5364, 5747, 6506
BstFNI	CGCG	3 cut(s)	2320, 2353, 6539
BstH2I	RGCGCY	2 cut(s)	754, 2101
BstHHI	GCGC	4 cut(s)	753, 2100, 2316, 5783
BstMAI	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5299, 5309, 5407
BstMCI	CGRYCG	2 cut(s)	223, 1400
BstNSI	RCATGY	4 cut(s)	259, 3509, 3860, 6098
BstPI	GGTNACC	2 cut(s)	4219, 5041
BstSFI	CTRYAG	5 cut(s)	1483, 2418, 2438, 4035, 4137
BstSLI	GKGCMC	3 cut(s)	2590, 4864, 6043
BstUI	CGCG	3 cut(s)	2320, 2353, 6539
BstV2I	GAAGAC	4 cut(s)	1501, 2924, 3396, 6403
BstX2I	RGATCY	9 cut(s)	182, 916, 1273, 2169, 2454, 3817, 4702, 5104, 6124
BstXI	CCANNNNNNTGG	2 cut(s)	3665, 4298
BstYI	RGATCY	9 cut(s)	182, 916, 1273, 2169, 2454, 3817, 4702, 5104, 6124
BstZI	CGGCCG	1 cut(s)	220
Bsu36I	CCTNAGG	1 cut(s)	6315
BtgI	CCRYGG	7 cut(s)	1322, 1732, 2926, 3587, 4084, 5465, 5983
BtgZI	GCGATG	1 cut(s)	317
BtrI	CACGTC	1 cut(s)	1959
BtsI	GCAGTG	1 cut(s)	3333
BveI	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
CaiI	CAGNNNCTG	2 cut(s)	695, 2756
CciI	TCATGA	3 cut(s)	3370, 3409, 4213
CfoI	GCGC	4 cut(s)	753, 2100, 2316, 5783
Cfr10I	RCCGGY	1 cut(s)	6429
CseI	GACGC	3 cut(s)	1171, 1368, 3436
CsiI	ACCWGGT	1 cut(s)	5155
CspCI	CAANNNNNGTGG	6 cut(s)	2116, 2151, 2908, 2943, 6381, 6416
DraI	TTTAAA	3 cut(s)	2944, 3930, 4986
DraIII	CACNNNGTG	2 cut(s)	5232, 5363
EagI	CGGCCG	1 cut(s)	220
Eam1104I	CTCTTC	4 cut(s)	3144, 3679, 3714, 5021
EarI	CTCTTC	4 cut(s)	3144, 3679, 3714, 5021
EciI	GGCGGA	2 cut(s)	253, 353
Ecl136II	GAGCTC	1 cut(s)	1624
EclXI	CGGCCG	1 cut(s)	220
Eco147I	AGGCCT	1 cut(s)	4886
Eco24I	GRGCYC	3 cut(s)	1626, 3439, 6260
Eco31I	GGTCTC	2 cut(s)	2150, 3602
Eco32I	GATATC	2 cut(s)	2562, 3376
Eco52I	CGGCCG	1 cut(s)	220
Eco53kI	GAGCTC	1 cut(s)	1624
Eco72I	CACGTG	2 cut(s)	1076, 2186
Eco81I	CCTNAGG	1 cut(s)	6315
Eco88I	CYCGRG	5 cut(s)	284, 853, 2727, 5255, 5888
Eco91I	GGTNACC	2 cut(s)	4219, 5041
EcoICRI	GAGCTC	1 cut(s)	1624
EcoNI	CCTNNNNNAGG	5 cut(s)	2164, 3273, 5364, 5747, 6506
EcoO109I	RGGNCCY	4 cut(s)	1663, 2039, 3549, 5797
EcoO65I	GGTNACC	2 cut(s)	4219, 5041
EcoRI	GAATTC	2 cut(s)	673, 3328
EcoRV	GATATC	2 cut(s)	2562, 3376
EcoT22I	ATGCAT	1 cut(s)	4951
EcoT38I	GRGCYC	3 cut(s)	1626, 3439, 6260
Esp3I	CGTCTC	1 cut(s)	2030
FauI	CCCGC	3 cut(s)	231, 4688, 5652
FauNDI	CATATG	1 cut(s)	839
FbaI	TGATCA	2 cut(s)	4555, 5539
FblI	GTMKAC	1 cut(s)	1461
FriOI	GRGCYC	3 cut(s)	1626, 3439, 6260
FspBI	CTAG	9 cut(s)	1196, 2297, 2652, 3486, 3555, 4467, 4530, 5528, 6293
GlaI	GCGC	4 cut(s)	752, 2099, 2315, 5782
GsaI	CCCAGC	6 cut(s)	527, 990, 994, 3582, 5526, 6423
GsuI	CTGGAG	6 cut(s)	668, 1455, 1701, 3641, 3864, 5847
HaeII	RGCGCY	2 cut(s)	754, 2101
HgaI	GACGC	3 cut(s)	1171, 1368, 3436
HhaI	GCGC	4 cut(s)	753, 2100, 2316, 5783
Hin1I	GRCGYC	1 cut(s)	1182
Hin6I	GCGC	4 cut(s)	751, 2098, 2314, 5781
HinP1I	GCGC	4 cut(s)	751, 2098, 2314, 5781
HincII	GTYRAC	4 cut(s)	1060, 5665, 5689, 6493
HindII	GTYRAC	4 cut(s)	1060, 5665, 5689, 6493
HindIII	AAGCTT	5 cut(s)	1877, 2601, 2837, 4777, 5869
HpaI	GTTAAC	2 cut(s)	1060, 5665
Hpy99I	CGWCG	2 cut(s)	215, 4361
Hsp92I	GRCGYC	1 cut(s)	1182
HspAI	GCGC	4 cut(s)	751, 2098, 2314, 5781
KflI	GGGWCCC	1 cut(s)	1663
Kpn2I	TCCGGA	2 cut(s)	45, 249
KroI	GCCGGC	1 cut(s)	6429
KroNI	GCCGGC	1 cut(s)	6431
Ksp22I	TGATCA	2 cut(s)	4555, 5539
KspAI	GTTAAC	2 cut(s)	1060, 5665
MabI	ACCWGGT	1 cut(s)	5155
MaeI	CTAG	9 cut(s)	1196, 2297, 2652, 3486, 3555, 4467, 4530, 5528, 6293
MbiI	CCGCTC	2 cut(s)	225, 283
MfeI	CAATTG	3 cut(s)	1140, 1929, 3384
MflI	RGATCY	9 cut(s)	182, 916, 1273, 2169, 2454, 3817, 4702, 5104, 6124
MhlI	GDGCHC	8 cut(s)	1003, 1291, 1626, 2590, 3439, 4864, 6043, 6260
MlsI	TGGCCA	7 cut(s)	621, 1023, 1442, 3669, 3695, 4787, 6058
MluNI	TGGCCA	7 cut(s)	621, 1023, 1442, 3669, 3695, 4787, 6058
MlyI	GAGTC	8 cut(s)	308, 577, 620, 1386, 1737, 4729, 5301, 6550
Mox20I	TGGCCA	7 cut(s)	621, 1023, 1442, 3669, 3695, 4787, 6058
Mph1103I	ATGCAT	1 cut(s)	4951
MroI	TCCGGA	2 cut(s)	45, 249
MroNI	GCCGGC	1 cut(s)	6429
MroXI	GAANNNNTTC	4 cut(s)	152, 155, 775, 4245
MscI	TGGCCA	7 cut(s)	621, 1023, 1442, 3669, 3695, 4787, 6058
MslI	CAYNNNNRTG	9 cut(s)	819, 1961, 2216, 2706, 2814, 2970, 3071, 3767, 4563
Msp20I	TGGCCA	7 cut(s)	621, 1023, 1442, 3669, 3695, 4787, 6058
MspA1I	CMGCKG	5 cut(s)	523, 895, 990, 4683, 6482
MspCI	CTTAAG	2 cut(s)	3416, 4961
MunI	CAATTG	3 cut(s)	1140, 1929, 3384
Mva1269I	GAATGC	3 cut(s)	2538, 3427, 3580
MvnI	CGCG	3 cut(s)	2320, 2353, 6539
NaeI	GCCGGC	1 cut(s)	6431
NciI	CCSGG	4 cut(s)	219, 299, 1401, 5063
NcoI	CCATGG	5 cut(s)	1322, 1732, 3587, 4084, 5983
NdeI	CATATG	1 cut(s)	839
NgoMIV	GCCGGC	1 cut(s)	6429
NheI	GCTAGC	1 cut(s)	5527
NmeAIII	GCCGAG	2 cut(s)	634, 5701
NmuCI	GTSAC	9 cut(s)	1414, 1954, 3293, 3906, 4219, 5041, 5566, 5945, 6350
NsiI	ATGCAT	1 cut(s)	4951
NspI	RCATGY	4 cut(s)	259, 3509, 3860, 6098
NspV	TTCGAA	1 cut(s)	3922
OliI	CACNNNNGTG	1 cut(s)	2216
PaeI	GCATGC	2 cut(s)	3509, 6098
PaeR7I	CTCGAG	2 cut(s)	853, 5888
PagI	TCATGA	3 cut(s)	3370, 3409, 4213
PasI	CCCWGGG	1 cut(s)	3431
PceI	AGGCCT	1 cut(s)	4886
PciI	ACATGT	1 cut(s)	3856
PcsI	WCGNNNNNNNCGW	3 cut(s)	95, 291, 6076
PctI	GAATGC	3 cut(s)	2538, 3427, 3580
PdiI	GCCGGC	1 cut(s)	6431
PdmI	GAANNNNTTC	4 cut(s)	152, 155, 775, 4245
PflFI	GACNNNGTC	2 cut(s)	1401, 1661
PflMI	CCANNNNNTGG	3 cut(s)	1328, 2774, 4090
PleI	GAGTC	8 cut(s)	308, 576, 619, 1386, 1736, 4729, 5301, 6549
PmaCI	CACGTG	2 cut(s)	1076, 2186
PmlI	CACGTG	2 cut(s)	1076, 2186
PpsI	GAGTC	8 cut(s)	308, 576, 619, 1386, 1736, 4729, 5301, 6549
Ppu21I	YACGTR	3 cut(s)	1076, 2186, 6203
PpuMI	RGGWCCY	3 cut(s)	1663, 2039, 5797
PscI	ACATGT	1 cut(s)	3856
PshBI	ATTAAT	1 cut(s)	3404
Psp124BI	GAGCTC	1 cut(s)	1626
Psp1406I	AACGTT	1 cut(s)	4228
Psp5II	RGGWCCY	3 cut(s)	1663, 2039, 5797
PspCI	CACGTG	2 cut(s)	1076, 2186
PspEI	GGTNACC	2 cut(s)	4219, 5041
PspFI	CCCAGC	6 cut(s)	523, 986, 990, 3578, 5522, 6419
PspPPI	RGGWCCY	3 cut(s)	1663, 2039, 5797
PspXI	VCTCGAGB	1 cut(s)	853
PstI	CTGCAG	3 cut(s)	1487, 2442, 4039
PstNI	CAGNNNCTG	2 cut(s)	695, 2756
PsuI	RGATCY	9 cut(s)	182, 916, 1273, 2169, 2454, 3817, 4702, 5104, 6124
PsyI	GACNNNGTC	2 cut(s)	1401, 1661
PvuII	CAGCTG	3 cut(s)	895, 990, 6482
RseI	CAYNNNNRTG	9 cut(s)	819, 1961, 2216, 2706, 2814, 2970, 3071, 3767, 4563
SacI	GAGCTC	1 cut(s)	1626
ScaI	AGTACT	3 cut(s)	2510, 3825, 4797
SchI	GAGTC	8 cut(s)	308, 577, 620, 1386, 1737, 4729, 5301, 6550
SduI	GDGCHC	8 cut(s)	1003, 1291, 1626, 2590, 3439, 4864, 6043, 6260
SexAI	ACCWGGT	1 cut(s)	5155
SfcI	CTRYAG	5 cut(s)	1483, 2418, 2438, 4035, 4137
SfiI	GGCCNNNNNGGCC	1 cut(s)	6064
Sfr274I	CTCGAG	2 cut(s)	853, 5888
SfuI	TTCGAA	1 cut(s)	3922
SlaI	CTCGAG	2 cut(s)	853, 5888
SmiMI	CAYNNNNRTG	9 cut(s)	819, 1961, 2216, 2706, 2814, 2970, 3071, 3767, 4563
SpeI	ACTAGT	1 cut(s)	4466
SphI	GCATGC	2 cut(s)	3509, 6098
SseBI	AGGCCT	1 cut(s)	4886
SspI	AATATT	3 cut(s)	1301, 1574, 4064
SspMI	CTAG	9 cut(s)	1196, 2297, 2652, 3486, 3555, 4467, 4530, 5528, 6293
SstI	GAGCTC	1 cut(s)	1626
StuI	AGGCCT	1 cut(s)	4886
TaqII	GACCGA	1 cut(s)	1967
TseFI	GTSAC	9 cut(s)	1414, 1954, 3293, 3906, 4219, 5041, 5566, 5945, 6350
Tsp45I	GTSAC	9 cut(s)	1414, 1954, 3293, 3906, 4219, 5041, 5566, 5945, 6350
TspGWI	ACGGA	6 cut(s)	22, 49, 389, 782, 2915, 4680
Tth111I	GACNNNGTC	2 cut(s)	1401, 1661
Van91I	CCANNNNNTGG	3 cut(s)	1328, 2774, 4090
Vha464I	CTTAAG	2 cut(s)	3416, 4961
VneI	GTGCAC	2 cut(s)	2586, 4860
VspI	ATTAAT	1 cut(s)	3404
XagI	CCTNNNNNAGG	5 cut(s)	2164, 3273, 5364, 5747, 6506
XapI	RAATTY	7 cut(s)	673, 2939, 3328, 4271, 4670, 4826, 6004
XbaI	TCTAGA	1 cut(s)	3554
XceI	RCATGY	4 cut(s)	259, 3509, 3860, 6098
XcmI	CCANNNNNNNNNTGG	5 cut(s)	629, 2494, 3340, 3677, 6051
XhoI	CTCGAG	2 cut(s)	853, 5888
XmiI	GTMKAC	1 cut(s)	1461
XmnI	GAANNNNTTC	4 cut(s)	152, 155, 775, 4245
XspI	CTAG	9 cut(s)	1196, 2297, 2652, 3486, 3555, 4467, 4530, 5528, 6293
ZrmI	AGTACT	3 cut(s)	2510, 3825, 4797
Zsp2I	ATGCAT	1 cut(s)	4951

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.