Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0008640

Physical Location & Seq

Genomic Coordinates Forward (+)

22933352 .. 22944346

Gene Structure 10995 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g31770.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6681 bp

ATGTTGGCGGCGAGCTCCGGTTCCGTTCTCCAACTCCGAACCAAGCCTTCCGTCCTCGCCTCGCCGCAGCTCAATGCCTCGCCGATTGCGCGGCTCAGCACCGGCAGAGCCGCCACCTCTCGGTCGGCGTCGAAAGCAATAGCGAACAAGTTCTTCGGGACTCGATTGAGAGCAGCCGCCGGATCCGAGAGGCTTCACCTATGGCGATCCGAAGGTCCCGGCCGTTCGCCGAAGCTCAAGGTGGTGGTCCGGTCCATGCTCTCCGCCGTGCCGGAGAAGCCGCAGGGGCTGTACGACCCGAAGATGGATAAGGACTCGTGCGGTGTCGGCTTCGTGGCGGAATTGTCCGGTGAAAGCAGCCGTAAGACGATTACGGATGCTTTGGAAATGCTGGTGAGGATGACACATAGAGGAGCTTGTGGATGTGAAACGAACACTGGTGATGGAGCTGGTGTTCTTGTGGCGATTCCTCACGATTTCTACAAGGAGGCTGCCAAAGATATTGGGTTTGAGCTTCCAGCGCTGGGTGAGTATGCTGTTGGTATGCTCTACTTGCCAACATCTGAGAGTCGTCGAGAAGAGAGCAAAAATGTGTTTACAAAGGTTGCCGAGTCTCTTGGGCATACCGTTCTCGGTTGGCGATCTGTTCCAACTGATAACTCAGATTTGGGGAACTCTGCTCTCCAGACGGAACCTGTTATTGAGCAAGTGTTCCTTACCCCAACTCCGAGGTCAAAAGTTGATCTTGAGCGCCAGATGTATATACTTAGAAGGGTTTCAATGGTTGCCATTCGTGCTGCATTAAACCTTCAATATGGTGGTGCTAAAGATTTCTACATATGTTCACTTTCATCGAGGACGGTTGTTTATAAGGGTCAGCTGAAACCCGAACAGCTGAAGGGTTACTATTATGCAGATCTTGGCAATGAAAGGTTTACGAGCTACATGGCCCTGGTTCATTCACGATTCTCAACAAATACATTTCCCAGCTGGGACCGTGCTCAGCCTATGCGTGTCATAGGCCACAATGGAGAAATCAACACCCTTAGAGGAAATGTTAACTGGATGAAGGCGCGTGAGGGACTGCTCAAGTGTACTGAACTTGGCCTTTCAAAAAATGAGTTGAAGAAGCTGTTACCAATTGTGGATGCCAGCTCTTCTGATTCAGGAGCATTTGATGGTGTCCTTGAGCTTCTGGTTAGAGCTGGCAGAAGCCTCCCTGAAGCTATTATGATGATGATTCCTGAAGCATGGCAAAATGACAAGAACATGGATCCTGATAAGAGAGCACTGTATGAATATTTTTCATCTCTCATGGAGCCATGGGATGGGCCAGCTCTAATATCATTTACTGATGGACGCTATCTGGGAGCTACATTGGATCGGAATGGACTCCGACCAGGTCGATTTTACGTCACCCACAGTGGACGAGTTATTATGGCGAGTGAAGTTGGGGTTGTAGACGTACCCCCAGAAGATGTCTGCAGGAAAGGAAGACTTAATCCTGGAATGATGCTTTTGGTTGATTTTGAGAATCATATAGTGGTCGATGATGAAGCCTTGAAGAAGCAATATTCACTGGCGAGGCCATATGGGGAGTGGCTTAAAAGGCAAAAGATTGAGCTCAAGGACATAGTTGATTCTGTTAATGAATCTGACAGGGTCCCTCCATCCATAGCTGGAGTTGCTCCTGCATCTACTGATGATGAGGACATGGAAAACATGGGAGTCCACGGTTTACTGGCACCATTGAAGGCTTTTGGTTACACAGTTGAAGCCTTGGAGATGTTGTTGCTTCCTATGGCAAAAGATGGTGTAGAGGCTCTTGGGTCAATGGGGAATGATACCCCTTTGGCTGTTATGTCCAATAGAGAAAAGCTTACATTTGAGTATTTCAAGCAAATGTTCGCTCAAGTAACAAACCCTCCAATTGATCCAATCAGAGAGAAGGTAGTCACTTCTATGGAATGTATGATCGGTCCAGAAGGTGATCTAACAGAAACCACTGAAGAACAATGCCATCGTCTCTCACTCAAAGGTCCTCTTTTAAACATTGAAGAAATGGAAGCGATAAAAAAAATGAACTATAGAGGTTGGCGCTGTAAGGTTCTTGATATAACGTACTCCAAGGAACGTGGTAGAAAGGGATTGGAGGAGACCTTGGATAGGATCTGTGCTGAAGCACGTGAGGCAATTAAAAAGGGATATACCACACTGGTGCTTTCAGACAGAGCCTTCTCCCCAAAACGTGTTGCTGTAAGCTCACTCTTGGCTGTTGGTGCTGTCCATCAACATCTAGTTAAAAATCTTGAGCGCACAAGGGTAGGGTTGATAATTGAATCTGCTGAGCCGCGTGAAGTACACCATTTCTGTACGTTAGTTGGATTCGGTGCAGATGCTATTTGCCCCTACCTGGCTGTAGAGGCCATTTGGAGACTGCAGGTTGATGGAAAGATCCCACCAAAATCTAATGGAACAATATATTCCAAGGCTGAACTGGTTAAAAAGTACTTCAAGGCAAGCAACTATGGAATGCAGAAGGTTCTTGCCAAGATGGGCATATCTACTTTGGCCTCTTACAAGGGTGCACAGATTTTTGAAGCTTTGGGTCTTTCCTCTGAAGTGATTGAGAGATGCTTTGTGGGAACTCCTAGTAGAGTCGAGGGGGCAACATTTGAGATGCTTGCTCGTGATGGACTTCATCTGCATGATTTGGCATTCCCCTCTCGGGCTTTTCCTCCCGGTAGTGCAGAAGCTGTGGCACTACCGAATCCTGGGGATTATCATTGGAGAAAAGGTGGTGAGGTTCACCTAAATGATCCTTTTGCTATTTCCAAGCTTCAGGAGGCTGCCAGAACTAACAGTGTGGCTGCTTATAAAGAATATTCCAAGCTCATTCACCAATTAAATAAAGCCTGCAATCTCCGTGGTCTTCTGAAATTTAAAAACACAGAGCAGCAAATTCACTTGGATGAAGTAGAACCTGCCAGTGAGATTGTTAAACGTTTCTGTACTGGGGCAATGAGTTATGGATCAATATCATTGGAGGCACACACTACATTGGCCATTGCTATGAACAGAATGGGGGGAAAGTCAAATACAGGTGAGGGAGGTGAGCAGCCCTCTCGTATGGAGCCTCTTCCCGATGGTTCAATGAATCCAAAGAGGAGTTCAATTAAGCAGGTTGCAAGTGGGAGATTTGGAGTTTCAAGTTACTATCTAACAAATGCTGATGAACTCCAAATAAAAATGGCTCAGGGGGCCAAACCTGGTGAAGGTGGTGAGCTTCCTGGCCACAAGGTTATTGGAGACATTGCTGTTACTAGGAATTCCACTGCTGGTGTGGGACTTATCAGTCCACCTCCCCATCATGATATCTACTCAATTGAAGACCTTGCTCAACTAATTCATGATCTTAAGAATGCTAACCCAGGGGCTCGAATCAGCGTCAAGTTAGTATCTGAAGCTGGTGTAGGTGTAGTTGCTAGTGGAGTTGTGAAGGGGCATGCCGATCATGTTTTAATTGCCGGACATGATGGAGGCACTGGGGCCTCTAGATGGACTGGCATTAAGAACGCTGGGCTTCCGTGGGAACTTGGCTTGGCTGAGACCCATCAGACCTTGGTTGCTAATGATCTTCGTGGCCGCACAGTTCTCCAGACAGATGGCCAACTAAAGACCGGAAGAGATGTGGCCATAGCCGCACTTCTTGGTGCTGAAGAGTTTGGGTTCAGCACAGCACCCCTCATAACCCTCGGCTGCATTATGATGCGGAAGTGCCACAAGAACACCTGTCCTGTTGGTATTGCAACTCAGGATCCAGTACTTCGTGAGAAGTTTGCTGGAGAACCTGAACATGTTATCAACTTTTTCTTCATGGTAGCTGAGGAGGTGAGAGAGATAATGGCACAACTTGGTTTTCGAACTCTAAATGAAATGGTTGGTCGTTCCGATATGCTTGAAGTCGATAAAGAAGTTACTAAAGACAATGAAAAGCTGAACAACATTGATTTATCCCTGTTACTTCGACCTGCTGCAGACATTCGACCTGAAGCTGCACAATATTGTGTTCAGAAACAGGACCATGGGTTGGACATGGCTCTGGACCACAAACTTATTTCTCTTTCTAATTCTGCTATAGAAAAGGCTGTTCCTGTATATTTTGAAACTCCGGTTTGCAATGTGAATCGTGCTGTTGGAACAATGCTTAGTCATGAGGTGACCAAACGTTACAATAGGCAAGGGCTTCCCGCAGACACCATTCATATCAAATTCAATGGAAGTGCAGGCCAGAGCCTTGGAGCTTTCCTCTGCCCTGGCATCACGCTTGAGCTTGAAGGTGACAGCAATGATTACGTTGGTAAAGGATTGTCTGGTGGCAAGATTATTGTTTACCCCCCCAAGGAAAGCAAGTTTGACCCGAAAGAAAACATTGTCATTGGTAATGTAGCTCTTTATGGTGCCACTAGTGGGGAGGCATATTTCAATGGAATGGCAGCAGAAAGATTCTGTGTTCGTAATTCCGGTGCTCGGGCAGTAGTTGAAGGTGTGGGTGATCATGGCTGTGAGTACATGACAGGTGGCACTGTTGTTGTGCTTGGGAAAACTGGCAGGAACTTTGCTGCTGGTATGAGTGGTGGCATAGCCTACGTTTTTGATGTCGATGGAAAATTTTTATCCAGATGCAATCCTGAGCTTGTCGATCTTGACAAGGTTGAAGAAGAAGAAGATATTCTGACTCTTAGGATGATGATACAGCAGCATCAACGCCATACAAAAAGCCTGCTGGCCAGTGAAGTACTGGCTGATTTTGAGAATCTTTTGCCTAAATTCATCAAGGTAATCCCCAGAGAGTACAAGCGTGCACTTGCAAATCTGAGGGAGGAGGCCTCCAAACAGGCAGTCGAGGACGCTGATGAAGAGGCTGAGAAGCAAGAGGAGGAGGAGCTTAAACTGAAAGAGAAAGATGCATTTGAAGAACTTAAGAAGATGGCATCTGCATCTTTAAATGAACTTAAGAACATGGCATCTGCATCCTTAAATGGGAATTCCAATCAGGTAAAAGTTCAGGTAGAAGATGCTGAAACATTGAAGAGGCCCAAAGAGGTCAGCAAAGCTGTTAAACACCGGGGTTTCATTTCTTATGAGCGTGAGGGCGTTCAATACAGGGATCCCAATGTTCGGATGAATGACTGGGACGAAGTTATGGAGGAAACTAAACCTGGCCCACTTGTGAATACTCAGTCAGCCCGTTGTATGGACTGTGGCACTCCTTTCTGCCATCAGGAGAACACTGGTTGTCCTCTTGGTAACAAAATACCTGAGTTCAATGAATTGGTGTACCAGAATAGGTGGCATGATGCATTAGAGAGACTTCTCGAGACAAATAATTTCCCTGAGTTTACTGGTCGAGTATGTCCGGCACCTTGTGAAGGTTCATGTGTTCTTGGTATCATTGAGAATCCTGTCTCTATCAAAAGCATTGAATGTGCAATCATAGACAAGGCTTTTGAGGAAGGTTGGATGGTACCACGACCTCCTGTCAAGAGAACTGGGAAAAAGGTTGCCATCGTCGGAAGTGGGCCTGCTGGGCTAGCTGCTGCTGATCAACTAAACAGAATAGGTCATACAGTGACAGTTTATGAGCGTGCTGATAGAATTGGAGGGCTTATGATGTATGGAGTGCCTAATATGAAAGCTGACAAAGTTGATGTAGTTCAGCGGCGGGTTAACCTTATGGCCGAGGAAGGTGTCAACTTTGTGGTCAATGCTAATGTTGGAAATGACTCATCCTATTCGTTTGACCGCCTTCGAGAGGAGAACAATGCGATCATTTTAGCAGTAGGCGCAACAAAACCAAGGGACCTTCCCGTGCCTGGGCGAGAGCTGTCAGGAGTTCATTTTGCTATGGAGTTTCTACACGCAAACACCAAAAGCTTGCTTGATAGCAATCTCGAGAATGGAAACTACATTTCTGCCAAGGGTAAGAAGGTTGTGGTCATTGGTGGAGGTGACACTGGCACTGATTGCATTGGAACCTCTGTTCGCCATGGCTGTACTGACATCGTAAATTTAGAGCTTCTCCCTCAGCCACCACAAACAAGGGCTCCAGGAAACCCTTGGCCGCAGTGGCCTCGTATCTTCCGAGTAGATTATGGGCACGCAGAAGTTGCAGCCAAGTTTGGCAAAGATCCAAGAACGTATGAGGTGCTGACCAAGAGGTTTGTGGGAGATGAGAATGGGGTTGTGAAGGGAATCGAGGTAGTACGTGTCAAATGGGAGAAGGATGCTACTGGCAAGTTCCAATTTAAGGAAATTGAGGGCTCTGAGGAGATAATTGAGGCGGACCTCGTCCTTCTAGCAATGGGGTTCCTTGGACCCGAGGCGGCGATTGCAGAGAAGTTGGGTTTGGAGTGTGACAATCGCTCAAACTTCAAGGCTGATTATGGTCGGTTCTCAACCAATGTGGATGGGGTCTTTGCTGCTGGGGATTGCCGGCGTGGTCAGTCCCTGGTGGTGTGGGCAATCTCAGAGGGACGGCAAGCAGCTGCACAGGTTGACAACTACCTCTGCAAGGAAGAAGAGGACCACACAGACAGCAACTCAAGCCCTGAATCTGATCTCTTGAAGCGGCATCAAGAAATCACCAAGAGCCAAAAGACAGTGGCAAGCACATCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015930 GO:0016040 GO:0016053 GO:0016491 GO:0016638 GO:0016639 GO:0019676 GO:0019740 GO:0019752 GO:0032502 GO:0040007 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045181 GO:0046394 GO:0046686 GO:0048589 GO:0050896 GO:0055114 GO:0060359 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00250 ko00910 ko01100 ko01110 ko01120 ko01130 ko01230 map00250 map00910 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

Fer4_20 GATase_2 GXGXG Glu_syn_central Glu_synthase Pyr_redox_2

Protein Analysis

2227

Amino Acids

244.09

Weight (kDa)

6.09

Isoelectric Point (pI)

36.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	9 - 227	1.5e-104	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	277 - 565	2.4e-112	Glutamate synthase central domain
Glu_synthase	PF01645	632 - 1000	3.5e-150	Conserved region in glutamate synthase
GXGXG	PF01493	1084 - 1270	6.6e-90	GXGXG motif
Fer4_20	PF14691	1423 - 1533	1.3e-21	Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
Pyr_redox_2	PF07992	1546 - 1872	1.8e-28	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	1550 - 1585	1.5e-08	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0008640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53460 AT5G53460 AT5G53460
fragaria_vesca	FvH4_7g31770 FvH4_7g31770 FvH4_7g31770
malus_domestica	MD01G1219500.v1.1 MD07G1290500.v1.1
prunus_persica	Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1
pyrus_communis	pycom01g22930 pycom07g26320
rosa_chinensis	RchiOBHm_Chr1g0381001
rosa_laevigata	RLG00000026253
rosa_multiflora	Rmu_ssc0000032.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278870
rosa_rugosa	Rorug01G0426400
rosa_samantha	Rh1AG450100 Rh1BG406100 Rh1CG420100 Rh1DG436600
rosa_wichuraiana	Rw1G039220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	870, 2877
AasI	GACNNNNNNGTC	1 cut(s)	3354
Acc36I	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
Acc65I	GGTACC	1 cut(s)	5527
AccB1I	GGYRCC	4 cut(s)	1744, 4460, 5422, 5527
AccB7I	CCANNNNNTGG	2 cut(s)	1328, 4090
AccI	GTMKAC	1 cut(s)	1461
AccII	CGCG	3 cut(s)	91, 1075, 2353
AclI	AACGTT	2 cut(s)	3004, 4228
AcoI	YGGCCR	9 cut(s)	220, 3063, 3292, 3643, 3667, 3693, 4788, 5739, 6122
AcsI	RAATTY	8 cut(s)	2939, 2961, 3328, 4271, 4670, 4829, 5047, 6070
AcvI	CACGTG	1 cut(s)	2186
AcyI	GRCGYC	1 cut(s)	128
AdeI	CACNNNGTG	1 cut(s)	5429
AfeI	AGCGCT	1 cut(s)	522
AflII	CTTAAG	3 cut(s)	3416, 4982, 5015
AflIII	ACRYGT	3 cut(s)	2248, 3856, 6268
AhdI	GACNNNNNGTC	1 cut(s)	5540
AhlI	ACTAGT	1 cut(s)	4466
AjuI	GAANNNNNNNTTGG	4 cut(s)	3154, 3186, 6392, 6424
AleI	CACNNNNGTG	1 cut(s)	2216
AloI	GAACNNNNNNTCC	4 cut(s)	438, 470, 6027, 6059
Alw21I	GWGCWC	7 cut(s)	17, 1003, 1291, 1626, 2590, 4531, 4867
Alw26I	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5365, 5375, 5473
Alw44I	GTGCAC	2 cut(s)	2586, 4863
AlwNI	CAGNNNCTG	3 cut(s)	289, 2756, 6511
Ama87I	CYCGRG	5 cut(s)	2727, 4530, 5378, 5954, 6380
Aor51HI	AGCGCT	1 cut(s)	522
ApaLI	GTGCAC	2 cut(s)	2586, 4863
ApoI	RAATTY	8 cut(s)	2939, 2961, 3328, 4271, 4670, 4829, 5047, 6070
ArsI	GACNNNNNNTTYG	4 cut(s)	4887, 4919, 5492, 5524
Asp700I	GAANNNNTTC	3 cut(s)	149, 775, 4731
Asp718I	GGTACC	1 cut(s)	5527
AspLEI	GCGC	7 cut(s)	91, 523, 753, 1075, 2100, 2316, 5849
AsuC2I	CCSGG	3 cut(s)	219, 2742, 5129
AsuII	TTCGAA	1 cut(s)	3922
AsuNHI	GCTAGC	1 cut(s)	5593
AvaI	CYCGRG	5 cut(s)	2727, 4530, 5378, 5954, 6380
BaeGI	GKGCMC	3 cut(s)	2590, 4867, 6162
BalI	TGGCCA	5 cut(s)	3065, 3294, 3669, 3695, 4790
BamHI	GGATCC	4 cut(s)	182, 1273, 3817, 5170
BanI	GGYRCC	4 cut(s)	1744, 4460, 5422, 5527
BanII	GRGCYC	5 cut(s)	17, 1626, 3439, 6109, 6326
BauI	CACGAG	2 cut(s)	316, 2688
BbrPI	CACGTG	1 cut(s)	2186
BbsI	GAAGAC	3 cut(s)	1501, 2924, 3396
Bbv12I	GWGCWC	7 cut(s)	17, 1003, 1291, 1626, 2590, 4531, 4867
BbvCI	CCTCAGC	2 cut(s)	3885, 6087
BceAI	ACGGC	4 cut(s)	207, 251, 345, 6554
BcgI	CGANNNNNNTGC	5 cut(s)	3107, 3141, 6116, 6150, 6657
BclI	TGATCA	2 cut(s)	4555, 5605
BcnI	CCSGG	3 cut(s)	219, 2742, 5129
BcoDI	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5365, 5375, 5473
BcuI	ACTAGT	1 cut(s)	4466
BfaI	CTAG	8 cut(s)	2297, 2652, 3324, 3486, 3555, 4467, 5594, 6359
BfmI	CTRYAG	6 cut(s)	1483, 2086, 2418, 2438, 4035, 4137
BfoI	RGCGCY	3 cut(s)	524, 754, 2101
BfrI	CTTAAG	3 cut(s)	3416, 4982, 5015
BfuAI	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
BglI	GCCNNNNNGGC	3 cut(s)	286, 5590, 6130
BglII	AGATCT	1 cut(s)	916
BlpI	GCTNAGC	3 cut(s)	95, 1002, 2346
BmcAI	AGTACT	3 cut(s)	2510, 3825, 4800
BmeRI	GACNNNNNGTC	1 cut(s)	5540
BmeT110I	CYCGRG	5 cut(s)	2727, 4530, 5378, 5954, 6380
BmrI	ACTGGG	4 cut(s)	3024, 3555, 5203, 5562
BmtI	GCTAGC	1 cut(s)	5597
BmuI	ACTGGG	4 cut(s)	3024, 3555, 5203, 5562
BpiI	GAAGAC	3 cut(s)	1501, 2924, 3396
BplI	GAGNNNNNCTC	6 cut(s)	3107, 3139, 4293, 4325, 4874, 4906
BpmI	CTGGAG	5 cut(s)	668, 1701, 3641, 3864, 6093
Bpu10I	CCTNAGC	4 cut(s)	3255, 3885, 4692, 6087
Bpu1102I	GCTNAGC	3 cut(s)	95, 1002, 2346
Bpu14I	TTCGAA	1 cut(s)	3922
BpuEI	CTTGAG	9 cut(s)	221, 767, 1073, 1208, 1610, 1896, 2330, 4349, 6589
BpuMI	CCSGG	3 cut(s)	219, 2742, 5129
BsaAI	YACGTR	2 cut(s)	2186, 6269
BsaBI	GATNNNNATC	2 cut(s)	3037, 5949
BsaHI	GRCGYC	1 cut(s)	128
BsaI	GGTCTC	2 cut(s)	2150, 3602
BsaWI	WCCGGW	6 cut(s)	17, 249, 347, 3680, 4171, 4523
Bse118I	RCCGGY	2 cut(s)	101, 6495
Bse3DI	GCAATG	7 cut(s)	931, 3027, 3066, 3312, 4186, 4354, 6369
Bse8I	GATNNNNATC	2 cut(s)	3037, 5949
BseJI	GATNNNNATC	2 cut(s)	3037, 5949
BseMI	GCAATG	7 cut(s)	931, 3027, 3066, 3312, 4186, 4354, 6369
BseSI	GKGCMC	3 cut(s)	2590, 4867, 6162
BseX3I	CGGCCG	1 cut(s)	220
BseYI	CCCAGC	6 cut(s)	523, 986, 990, 3578, 5588, 6485
BsgI	GTGCAG	5 cut(s)	2412, 2769, 4041, 4305, 6534
Bsh1236I	CGCG	3 cut(s)	91, 1075, 2353
Bsh1285I	CGRYCG	2 cut(s)	125, 223
BshNI	GGYRCC	4 cut(s)	1744, 4460, 5422, 5527
BsiEI	CGRYCG	2 cut(s)	125, 223
BsiHKAI	GWGCWC	7 cut(s)	17, 1003, 1291, 1626, 2590, 4531, 4867
BsiHKCI	CYCGRG	5 cut(s)	2727, 4530, 5378, 5954, 6380
BsmAI	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5365, 5375, 5473
BsmBI	CGTCTC	1 cut(s)	2030
BsmI	GAATGC	3 cut(s)	2538, 2717, 3427
Bso31I	GGTCTC	2 cut(s)	2150, 3602
BsoBI	CYCGRG	5 cut(s)	2727, 4530, 5378, 5954, 6380
Bsp119I	TTCGAA	1 cut(s)	3922
Bsp1720I	GCTNAGC	3 cut(s)	95, 1002, 2346
Bsp19I	CCATGG	3 cut(s)	1322, 4084, 6049
BspFNI	CGCG	3 cut(s)	91, 1075, 2353
BspHI	TCATGA	3 cut(s)	3370, 3409, 4213
BspMAI	CTGCAG	3 cut(s)	1487, 2442, 4039
BspMI	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
BspOI	GCTAGC	1 cut(s)	5597
BspQI	GCTCTTC	1 cut(s)	1162
BspT104I	TTCGAA	1 cut(s)	3922
BspT107I	GGYRCC	4 cut(s)	1744, 4460, 5422, 5527
BspTI	CTTAAG	3 cut(s)	3416, 4982, 5015
BspTNI	GGTCTC	2 cut(s)	2150, 3602
BsrDI	GCAATG	7 cut(s)	931, 3027, 3066, 3312, 4186, 4354, 6369
BsrFI	RCCGGY	2 cut(s)	101, 6495
BssAI	RCCGGY	2 cut(s)	101, 6495
BssNI	GRCGYC	1 cut(s)	128
BssSI	CACGAG	2 cut(s)	316, 2688
Bst2BI	CACGAG	2 cut(s)	316, 2688
Bst6I	CTCTTC	8 cut(s)	573, 1162, 3144, 3679, 3714, 4914, 5087, 6576
BstACI	GRCGYC	1 cut(s)	128
BstAFI	CTTAAG	3 cut(s)	3416, 4982, 5015
BstAPI	GCANNNNNTGC	2 cut(s)	6027, 6170
BstBAI	YACGTR	2 cut(s)	2186, 6269
BstBI	TTCGAA	1 cut(s)	3922
BstDSI	CCRYGG	6 cut(s)	1322, 1732, 2926, 3587, 4084, 6049
BstEII	GGTNACC	1 cut(s)	4219
BstENI	CCTNNNNNAGG	5 cut(s)	2164, 3273, 5430, 5813, 6572
BstFNI	CGCG	3 cut(s)	91, 1075, 2353
BstH2I	RGCGCY	3 cut(s)	524, 754, 2101
BstHHI	GCGC	7 cut(s)	91, 523, 753, 1075, 2100, 2316, 5849
BstMAI	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5365, 5375, 5473
BstMCI	CGRYCG	2 cut(s)	125, 223
BstNSI	RCATGY	2 cut(s)	3509, 3860
BstPI	GGTNACC	1 cut(s)	4219
BstSFI	CTRYAG	6 cut(s)	1483, 2086, 2418, 2438, 4035, 4137
BstSLI	GKGCMC	3 cut(s)	2590, 4867, 6162
BstUI	CGCG	3 cut(s)	91, 1075, 2353
BstV2I	GAAGAC	3 cut(s)	1501, 2924, 3396
BstX2I	RGATCY	8 cut(s)	182, 916, 1273, 2169, 2454, 3817, 5170, 6190
BstXI	CCANNNNNNTGG	2 cut(s)	3665, 4298
BstYI	RGATCY	8 cut(s)	182, 916, 1273, 2169, 2454, 3817, 5170, 6190
BstZI	CGGCCG	1 cut(s)	220
BtgI	CCRYGG	6 cut(s)	1322, 1732, 2926, 3587, 4084, 6049
BtsI	GCAGTG	2 cut(s)	3333, 6134
BveI	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
CaiI	CAGNNNCTG	3 cut(s)	289, 2756, 6511
CciI	TCATGA	3 cut(s)	3370, 3409, 4213
CfoI	GCGC	7 cut(s)	91, 523, 753, 1075, 2100, 2316, 5849
Cfr10I	RCCGGY	2 cut(s)	101, 6495
CseI	GACGC	4 cut(s)	117, 1368, 3436, 4919
CsiI	ACCWGGT	2 cut(s)	1399, 3268
CspCI	CAANNNNNGTGG	6 cut(s)	2116, 2151, 2908, 2943, 6447, 6482
DraI	TTTAAA	3 cut(s)	2049, 2944, 5007
DraIII	CACNNNGTG	1 cut(s)	5429
DrdI	GACNNNNNNGTC	1 cut(s)	3354
DriI	GACNNNNNGTC	1 cut(s)	5540
DseDI	GACNNNNNNGTC	1 cut(s)	3354
EaeI	YGGCCR	9 cut(s)	220, 3063, 3292, 3643, 3667, 3693, 4788, 5739, 6122
EagI	CGGCCG	1 cut(s)	220
Eam1104I	CTCTTC	8 cut(s)	573, 1162, 3144, 3679, 3714, 4914, 5087, 6576
Eam1105I	GACNNNNNGTC	1 cut(s)	5540
EarI	CTCTTC	8 cut(s)	573, 1162, 3144, 3679, 3714, 4914, 5087, 6576
EciI	GGCGGA	3 cut(s)	253, 353, 6359
Ecl136II	GAGCTC	2 cut(s)	15, 1624
EclXI	CGGCCG	1 cut(s)	220
Eco147I	AGGCCT	1 cut(s)	4889
Eco24I	GRGCYC	5 cut(s)	17, 1626, 3439, 6109, 6326
Eco31I	GGTCTC	2 cut(s)	2150, 3602
Eco32I	GATATC	1 cut(s)	3376
Eco47III	AGCGCT	1 cut(s)	522
Eco52I	CGGCCG	1 cut(s)	220
Eco53kI	GAGCTC	2 cut(s)	15, 1624
Eco72I	CACGTG	1 cut(s)	2186
Eco88I	CYCGRG	5 cut(s)	2727, 4530, 5378, 5954, 6380
Eco91I	GGTNACC	1 cut(s)	4219
EcoICRI	GAGCTC	2 cut(s)	15, 1624
EcoNI	CCTNNNNNAGG	5 cut(s)	2164, 3273, 5430, 5813, 6572
EcoO109I	RGGNCCY	5 cut(s)	215, 1663, 2039, 3549, 5863
EcoO65I	GGTNACC	1 cut(s)	4219
EcoRI	GAATTC	2 cut(s)	3328, 5047
EcoRV	GATATC	1 cut(s)	3376
EcoT22I	ATGCAT	2 cut(s)	4972, 5365
EcoT38I	GRGCYC	5 cut(s)	17, 1626, 3439, 6109, 6326
Esp3I	CGTCTC	1 cut(s)	2030
FalI	AAGNNNNNCTT	8 cut(s)	699, 731, 729, 761, 1482, 1514, 2028, 2060
FauI	CCCGC	2 cut(s)	4258, 5718
FauNDI	CATATG	2 cut(s)	839, 1591
FbaI	TGATCA	2 cut(s)	4555, 5605
FblI	GTMKAC	1 cut(s)	1461
FriOI	GRGCYC	5 cut(s)	17, 1626, 3439, 6109, 6326
FspBI	CTAG	8 cut(s)	2297, 2652, 3324, 3486, 3555, 4467, 5594, 6359
GlaI	GCGC	7 cut(s)	90, 522, 752, 1074, 2099, 2315, 5848
GsaI	CCCAGC	6 cut(s)	527, 990, 994, 3582, 5592, 6489
GsuI	CTGGAG	5 cut(s)	668, 1701, 3641, 3864, 6093
HaeII	RGCGCY	3 cut(s)	524, 754, 2101
HgaI	GACGC	4 cut(s)	117, 1368, 3436, 4919
HhaI	GCGC	7 cut(s)	91, 523, 753, 1075, 2100, 2316, 5849
Hin1I	GRCGYC	1 cut(s)	128
Hin6I	GCGC	7 cut(s)	89, 521, 751, 1073, 2098, 2314, 5847
HinP1I	GCGC	7 cut(s)	89, 521, 751, 1073, 2098, 2314, 5847
HincII	GTYRAC	4 cut(s)	1060, 5731, 5755, 6559
HindII	GTYRAC	4 cut(s)	1060, 5731, 5755, 6559
HindIII	AAGCTT	4 cut(s)	1877, 2601, 2837, 5935
HpaI	GTTAAC	2 cut(s)	1060, 5731
Hpy99I	CGWCG	3 cut(s)	133, 576, 5576
Hsp92I	GRCGYC	1 cut(s)	128
HspAI	GCGC	7 cut(s)	89, 521, 751, 1073, 2098, 2314, 5847
KflI	GGGWCCC	1 cut(s)	1663
KpnI	GGTACC	1 cut(s)	5531
KroI	GCCGGC	1 cut(s)	6495
KroNI	GCCGGC	1 cut(s)	6497
Ksp22I	TGATCA	2 cut(s)	4555, 5605
KspAI	GTTAAC	2 cut(s)	1060, 5731
LguI	GCTCTTC	1 cut(s)	1162
MabI	ACCWGGT	2 cut(s)	1399, 3268
MaeI	CTAG	8 cut(s)	2297, 2652, 3324, 3486, 3555, 4467, 5594, 6359
MfeI	CAATTG	3 cut(s)	1140, 1929, 3384
MflI	RGATCY	8 cut(s)	182, 916, 1273, 2169, 2454, 3817, 5170, 6190
MlsI	TGGCCA	5 cut(s)	3065, 3294, 3669, 3695, 4790
MluNI	TGGCCA	5 cut(s)	3065, 3294, 3669, 3695, 4790
MlyI	GAGTC	9 cut(s)	154, 308, 577, 620, 1386, 1737, 2667, 4732, 5780
MmeI	TCCRAC	9 cut(s)	55, 674, 1420, 2362, 4071, 4177, 5501, 5554, 5758
Mox20I	TGGCCA	5 cut(s)	3065, 3294, 3669, 3695, 4790
Mph1103I	ATGCAT	2 cut(s)	4972, 5365
MroNI	GCCGGC	1 cut(s)	6495
MroXI	GAANNNNTTC	3 cut(s)	149, 775, 4731
MscI	TGGCCA	5 cut(s)	3065, 3294, 3669, 3695, 4790
MslI	CAYNNNNRTG	8 cut(s)	1961, 2216, 2706, 2814, 2970, 3071, 3767, 4563
Msp20I	TGGCCA	5 cut(s)	3065, 3294, 3669, 3695, 4790
MspA1I	CMGCKG	5 cut(s)	880, 895, 990, 5722, 6548
MspCI	CTTAAG	3 cut(s)	3416, 4982, 5015
MunI	CAATTG	3 cut(s)	1140, 1929, 3384
Mva1269I	GAATGC	3 cut(s)	2538, 2717, 3427
MvnI	CGCG	3 cut(s)	91, 1075, 2353
NaeI	GCCGGC	1 cut(s)	6497
NciI	CCSGG	3 cut(s)	219, 2742, 5129
NcoI	CCATGG	3 cut(s)	1322, 4084, 6049
NdeI	CATATG	2 cut(s)	839, 1591
NgoMIV	GCCGGC	1 cut(s)	6495
NheI	GCTAGC	1 cut(s)	5593
NmeAIII	GCCGAG	3 cut(s)	634, 3735, 5767
NmuCI	GTSAC	7 cut(s)	1414, 1954, 4219, 4340, 5632, 6011, 6416
NsiI	ATGCAT	2 cut(s)	4972, 5365
NspI	RCATGY	2 cut(s)	3509, 3860
NspV	TTCGAA	1 cut(s)	3922
OliI	CACNNNNGTG	1 cut(s)	2216
PaeI	GCATGC	1 cut(s)	3509
PaeR7I	CTCGAG	2 cut(s)	5378, 5954
PagI	TCATGA	3 cut(s)	3370, 3409, 4213
PasI	CCCWGGG	1 cut(s)	3431
PceI	AGGCCT	1 cut(s)	4889
PciI	ACATGT	1 cut(s)	3856
PciSI	GCTCTTC	1 cut(s)	1162
PcsI	WCGNNNNNNNCGW	1 cut(s)	6142
PctI	GAATGC	3 cut(s)	2538, 2717, 3427
PdiI	GCCGGC	1 cut(s)	6497
PdmI	GAANNNNTTC	3 cut(s)	149, 775, 4731
PflFI	GACNNNGTC	3 cut(s)	1401, 1661, 6350
PflMI	CCANNNNNTGG	2 cut(s)	1328, 4090
PfoI	TCCNGGA	2 cut(s)	1504, 6109
PleI	GAGTC	9 cut(s)	154, 308, 576, 619, 1386, 1736, 2666, 4732, 5780
PmaCI	CACGTG	1 cut(s)	2186
PmlI	CACGTG	1 cut(s)	2186
PpsI	GAGTC	9 cut(s)	154, 308, 576, 619, 1386, 1736, 2666, 4732, 5780
Ppu21I	YACGTR	2 cut(s)	2186, 6269
PpuMI	RGGWCCY	4 cut(s)	215, 1663, 2039, 5863
PscI	ACATGT	1 cut(s)	3856
PsiI	TTATAA	2 cut(s)	870, 2877
Psp124BI	GAGCTC	2 cut(s)	17, 1626
Psp1406I	AACGTT	2 cut(s)	3004, 4228
Psp5II	RGGWCCY	4 cut(s)	215, 1663, 2039, 5863
PspCI	CACGTG	1 cut(s)	2186
PspEI	GGTNACC	1 cut(s)	4219
PspFI	CCCAGC	6 cut(s)	523, 986, 990, 3578, 5588, 6485
PspPPI	RGGWCCY	4 cut(s)	215, 1663, 2039, 5863
PstI	CTGCAG	3 cut(s)	1487, 2442, 4039
PstNI	CAGNNNCTG	3 cut(s)	289, 2756, 6511
PsuI	RGATCY	8 cut(s)	182, 916, 1273, 2169, 2454, 3817, 5170, 6190
PsyI	GACNNNGTC	3 cut(s)	1401, 1661, 6350
PvuII	CAGCTG	4 cut(s)	880, 895, 990, 6548
RseI	CAYNNNNRTG	8 cut(s)	1961, 2216, 2706, 2814, 2970, 3071, 3767, 4563
SacI	GAGCTC	2 cut(s)	17, 1626
SapI	GCTCTTC	1 cut(s)	1162
ScaI	AGTACT	3 cut(s)	2510, 3825, 4800
SchI	GAGTC	9 cut(s)	154, 308, 577, 620, 1386, 1737, 2667, 4732, 5780
SexAI	ACCWGGT	2 cut(s)	1399, 3268
SfcI	CTRYAG	6 cut(s)	1483, 2086, 2418, 2438, 4035, 4137
SfiI	GGCCNNNNNGGCC	1 cut(s)	6130
Sfr274I	CTCGAG	2 cut(s)	5378, 5954
SfuI	TTCGAA	1 cut(s)	3922
SlaI	CTCGAG	2 cut(s)	5378, 5954
SmiMI	CAYNNNNRTG	8 cut(s)	1961, 2216, 2706, 2814, 2970, 3071, 3767, 4563
SpeI	ACTAGT	1 cut(s)	4466
SphI	GCATGC	1 cut(s)	3509
SseBI	AGGCCT	1 cut(s)	4889
SspI	AATATT	4 cut(s)	1301, 1574, 2885, 4064
SspMI	CTAG	8 cut(s)	2297, 2652, 3324, 3486, 3555, 4467, 5594, 6359
SstI	GAGCTC	2 cut(s)	17, 1626
StuI	AGGCCT	1 cut(s)	4889
TaqII	GACCGA	2 cut(s)	111, 1967
TatI	WGTACW	9 cut(s)	1094, 2359, 2508, 3011, 3823, 4569, 4798, 4854, 6056
TseFI	GTSAC	7 cut(s)	1414, 1954, 4219, 4340, 5632, 6011, 6416
Tsp45I	GTSAC	7 cut(s)	1414, 1954, 4219, 4340, 5632, 6011, 6416
TspGWI	ACGGA	6 cut(s)	13, 40, 389, 704, 2915, 3576
Tth111I	GACNNNGTC	3 cut(s)	1401, 1661, 6350
Van91I	CCANNNNNTGG	2 cut(s)	1328, 4090
Vha464I	CTTAAG	3 cut(s)	3416, 4982, 5015
VneI	GTGCAC	2 cut(s)	2586, 4863
XagI	CCTNNNNNAGG	5 cut(s)	2164, 3273, 5430, 5813, 6572
XapI	RAATTY	8 cut(s)	2939, 2961, 3328, 4271, 4670, 4829, 5047, 6070
XbaI	TCTAGA	1 cut(s)	3554
XceI	RCATGY	2 cut(s)	3509, 3860
XcmI	CCANNNNNNNNNTGG	7 cut(s)	1596, 2494, 3302, 3340, 4798, 6117, 6223
XhoI	CTCGAG	2 cut(s)	5378, 5954
XmiI	GTMKAC	1 cut(s)	1461
XmnI	GAANNNNTTC	3 cut(s)	149, 775, 4731
XspI	CTAG	8 cut(s)	2297, 2652, 3324, 3486, 3555, 4467, 5594, 6359
ZrmI	AGTACT	3 cut(s)	2510, 3825, 4800
Zsp2I	ATGCAT	2 cut(s)	4972, 5365

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.