Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0008640

Physical Location & Seq

Genomic Coordinates Forward (+)

66416683 .. 66427311

Gene Structure 10629 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ60420

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6630 bp

ATGTTGGCGGCGAGTTCCGGCGCTGGTGGTTCCGTTCTCCAACTCCGGACCAAACCTTCCGTCCTCGCTTCACCTCAGCTCAATGCCTCGCCGATAGCGAGGCTAAGCACCAGAGCCACTGCTCGCTCTGGTTCCAAAGCTATTGCGAAGAAGTTCTTCGGGACGAGATTGAGGGCCGCCGGATCCGACAGGCTGCACTTATGGCGATCCGACGGTCCCGGCCGCTCGCCGAAGCTGCGGGTGGTGGTCCGGAACATGCTCTCTGCCGTGCCGGAGAAGCCGCTCGGGCTATACGACCCGGCGATGGATAAGGACTCGTGCGGTGTTGGCTTCGTGGCGGAATTGTCCGGTGAAAGCAGCCGTAAAACGATTACGGATGCTCTGGAAATGTTGGTGCGGATGACACACAGAGGAGCATGTGGATGTGAAACAAACACTGGTGATGGAGCTGGTGTTCTTGTGGCGATTCCTCACGATTTCTATAAGGAGGCTGCCAAAGACAATGGGTTTGAGCTTCCACCGCTGGGTGAGTATGCTGTTGGTATGCTTTACTTGCCTACATCGGAGAGTCGTAGAGAGGAGAGCAAAAATGTGTTTACAAAGGTTGCCGAGTCTCTTGGCCATACTGTTCTTGGGTGGCGATCAGTTCCCACTGATAACTCAGATTTGGGGAATTCTGCTCTCCAGACAGAACCTGTTATTGAGCAAGTGTTCCTTACTCCAACTCCGAGGTCGAAAGTTGATCTTGAGCGCCAGATGTACATACTTAGAAGGGTTTCAATGGTTGCCATCCGTGCTGCATTAAACCTTCAACATGGTGGTGCTAAAGATTTCTACATATGTTCACTTTCCTCGAGGACGATTGTTTACAAGGGTCAGCTGAAACCCGAACAGCTGAAGGGTTACTATTATGCAGATCTTGGCAATGAAAGGTTTACGAGCTACATGGCCCTGGTACATTCTCGATTCTCAACAAATACTTTTCCCAGCTGGGATCGTGCTCAGCCCATGCGTGTCATTGGCCACAATGGAGAAATCAACACCCTTAGAGGAAATGTTAACTGGATGAAGGCACGTGAGGGACTGCTTAAGTGTACTGAACTTGGTTTATCAAAAAATGAGTTAAAGAAGCTGTTACCAATTGTGGATGCCAGTTCATCCGATTCAGGAGCTTTTGATGGTGTCCTTGAGCTTCTAGTTCGAGCTGGCAGAAGCCTCCCTGAAGCTATTATGATGATGATTCCTGAAGCATGGCAAAATGACAAAAACATGGATCCTGATAGGAGAGCACTGTATGAATATTTCTCATCTCTCATGGAGCCATGGGATGGGCCAGCTCTAATATCATTTACTGATGGACGCTATCTGGGAGCTACATTGGATCGGAATGGACTCCGACCGGGTCGCTTTTACGTCACCCACAGTGGACGAGTTATTATGGCCAGTGAAGTTGGGGTTGTAGATGTACCTCCAGAAGATGTCTGCAGGAAAGGAAGACTTAACCCTGGAATGATGCTTTTGGTGGATTTTGAGAATCATATAGTGGTAGATGATGAAGCCTTGAAGCAGCAATATTCACTGGCAAGGCCGTATGGGGAGTGGCTTAAAAGGCAGAAGATTGAGCTCAAGGACATAGTTGATTCTGTTCATGAATCTGACAGGGTGCCTCCATCCATAGCTGGAGTTGTTCCTGCATCTACTGATGATGACAACATGGAAAACATGGGAGTCCATGGTTTACTGGCACCACTGAAGGCTTTTGGTTACACAGTTGAAGCCTTGGAAATGTTGTTGCTTCCTATGGCAAAAGATGGTGTAGAGGCTCTTGGGTCAATGGGAAATGATACCCCCTTGGCTGTTATGTCCAATAGAGAAAAGCTTACATTTGAGTATTTCAAGCAAATGTTCGCCCAAGTAACAAACCCTCCAATTGATCCAATTCGAGAAAAGGTAGTCACATCTATGGAATGTATGATCGGTCCAGAAGGTGATCTAACAGAAACCACTGAAGAACAATGCCATCGTCTCTCACTCAAAGGTCCTCTTTTAAGCATTGAGGAAATGGAGGCCATCAAAAAAATGAATTACAGAGGTTGGCGCTGTAAGGTTCTTGACATAACTTACTCCAAGGAACGTGGTAGAAAGGGATTGGAGGAGACCTTGGATAGGATCTGTGCTGAAGCACGCGAGGCAATTAAAAAGGGATATACCACACTGGTGCTTTCAGACAGAGCCTTCTCCCCAAAATGTGTTGCTGTAAGCTCACTCTTGGCTGTTGGTGCTGTTCATCAACATCTAGTTAAAAATCTTGAGCGCACGCGAGTAGGGTTGATAATTGAATCTGCTGAGCCACGTGAGGTACACCATTTCTGTACATTAGTTGGATTCGGTGCAGATGCCATCTGCCCCTACCTGGCTGTAGAGGCTATTTGGAGACTGCAGGTTGATGGAAAGATCCCACCAAAATCTAATGGTACAATATATTCCAAGGATGAACTGGTTAAAAAGTACTTCAAAGCAAGCAACTATGGAATGCAGAAGGTTCTTGCCAAGATGGGGATATCTACTTTGGCCTCTTACAAGGGTGCACAGATTTTTGAAGCTTTGGGTCTTTCATCTGAAGTGATTGAGAGGTGCTTTGCAGGAACTCCTAGTAGAGTTGAGGGGGCAACATTTGAGATGCTTGCTCGTGATGGACTTCATCTGCATGATTTGGCATTTCCCTCTCGGGCTTTTCCTCCGGGTAGTGCAGAAGCTGTGGCACTACCAAATCCAGGGGATTATCATTGGAGAAAAGGTGGTGAAGTTCACCTAAATGATCCTTTTGCTATTTCCAAGCTTCAGGAGGCTGCCAGAACTAACAGTGTGGCGGCATATAAAGAATACTCCAAGCTCATTCATCAATTAAATAAAGCCTGCAATCTCCGTGGTCTTCTGAAATTTAAAAACACAGAGCAGCAAATTGACTTGGATGAAGTGGAACCTGCCAGTGAAATTGTTAAACGGTTCTGTACTGGGGCAATGAGTTATGGATCAATATCATTGGAGGCACACACTACACTGGCCATTGCTATGAACAGAATGGGAGGAAAGTCAAATACAGGTGAGGGAGGTGAGCAGCCCTCTCGTATGGAGCCTCTTCCTGATGGTTCAAGGAATCCAAAGAGGAGTGCAATTAAGCAGGTTGCAAGTGGGAGATTTGGAGTTTCAAGTTACTATCTTACAAATGCTGATGAACTCCAAATAAAAATGGCTCAGGGGGCCAAGCCCGGTGAAGGAGGTGAGCTTCCTGGTCACAAGGTTATTGGAGACATTGCTGTTACCAGGAATTCCACTGCTGGTGTGGGACTTATCAGCCCACCTCCCCATCATGATATCTACTCAATTGAAGACCTTGCTCAATTAATTCATGATCTTAAGAATGCTAACCCAGGGGCTCGAATAAGCGTCAAGTTAGTGTCTGAAGCTGGTGTAGGAGTAGTTGCTAGTGGAGTTGTGAAGGGGCATGCTGACCATGTTTTAATTGCCGGACATGATGGAGGCACTGGTGCCTCCAGATGGACTGGCATTAAGAATGCTGGGCTTCCATGGGAACTTGGCTTGGCTGAGACCCATCAAACCCTGGTTGCTAATGATCTTCGTGGCCGCACAGTTCTCCAGACAGATGGCCAACTAAAAACAGGAAGAGATGTGGCCATAGCCGCACTTCTTGGTGCAGAAGAGTTTGGGTTCAGCACGGCACCCCTCATAACCCTTGGCTGCATTATGATGCGGAAGTGCCACAAGAACACCTGTCCTGTCGGAATTGCAACCCAAGATCCAGTACTTCGTGAGAAGTTTGCCGGAGAACCTGAACATGTTATCAACTTTTTCTTCATGGTAGCTGAGGAGGTGAGAGAGATAATGTCACAACTTGGTTTTCGAACTTTAAATGAAATGGTTGGTCGTTCAGATATGCTTGAAGTTGACAAAGAAGTTACTAAAGGCAATGAGAAGCTGGACAACATTGATCTTTCCCTGTTACTTAGACCTGCTGCAGACATTCGGCCAGAAGCTGCACAATATTGTGTTCAGAAACAGGACCATGGGTTGGACATGGCTCTTGACCACAAACTTATTTCGCTGTCCAGTTCTGCTATAGAGAAGGCTCTTCCTGCATACTTTGAAACACCGATTTGCAATGTGAATCGTGCTGTTGGAACAATGCTTAGTCATGAGGTGACCAAACGTTACAACAGGGAAGGGCTTCCAGCAGACACAATTCATATCAAATTCATTGGAAGTGCAGGCCAGAGCCTTGGAGCTTTCCTCTGCCCTGGCATCACACTTGAGCTTGAAGGTGACAGCAATGATTACGTTGGTAAAGGATTGTCTGGTGGCAAGATCGTAGTGTATCCCCCCAAGGGAAGCAAGTTTGACCCGAAAGACAACATTGTCATTGGTAATGTAGCTCTTTATGGTGCCACTAGTGGGGAGGCATATTTCAATGGAATGGCAGCAGAAAGATTCTGTGTTCGCAATTCAGGGGCAAGGGCAGTAGTTGAAGGTGTCGGTGATCATGGCTGTGAGTACATGACAGGTGGCACTGTTGTTGTGCTTGGGAAAACTGGCAGGAACTTTGCTGCTGGTATGAGTGGTGGCATAGCCTACGTTCTTGATGTGGATGGAAAATTTTTGTCCCGATGCAATCCTGAGCTTGTAGATCTTGATAAAGTTGAAGGAGAAGATAGTATGACTCTTAAGATGATGATACAGCAGCATCAGCGTCATACAAAAAGCTTGCTGGCCAGTGAAGTACTGGCTGATTTTGAGAATCTTTTGCCTAAATTCATCAAGGTTATCCCCAGAGAGTACAAGCGTGCACTTGCAAACCTGAGGGAGGAGGCCACCAAACAGGCAGTTGATCAAGCTGATGAAGAAGCTCAGGAGCAAGAGGAAGAGGTGGAAATAAAGGGGAAAGATGCATTTGAAGAACTTAAGAAGATGGCATCTGCATCTTTAAATGGGACATCCAATCAGGTAGAAGATGCTGAAACATTGAAGAGGCCCAGTGAGGTCAGCAAAGCTGTTAAACACCGGGGTTTCATTTCTTATGAGCGTGAGGGCGTTCAATACAGGGATCCCAAAGTTCGGATGAATGACTGGAAGGAAGTTATGGAGGAAACTAAACCTGGTCCACTTGTGAATACACAGTCAGCACGTTGCATGGACTGTGGCACTCCTTTCTGCCATCAGGAAAACACTGGGTGTCCTCTTGGTAACAAAATACCTGAGTTCAATGAATTGGTGTACCAGAATAGGTGGCATGAAGCATTAGAGAGACTCCTCGAGACAAATAACTTCCCTGAGTTTACTGGTCGAGTTTGCCCAGCACCTTGTGAAGGTTCATGTGTTCTGGGTATTATTGAGAATCCTGTCTCTATTAAAAGCATTGAATGTGCCATCATAGACAAGGCTTTTGAGGAAGGTTGGATGGTGCCACGGCCTCCACTCAAGAGAACTGGGAAAAAGGTTGCCATTGTCGGAAGTGGGCCTGCTGGGCTAGCTGCTGCTGATCAACTAAACAGAATAGGTCATACAGTGACAGTTTACGAGCGTGCTGATAGAATTGGAGGGCTGATGATGTATGGAGTGCCTAATATGAAGGCTGACAAAGTTGATATAGTTCAACGGCGGGTTAACCTTATGGCCGAGGAAGGTGTCAACTTTGTGGTCAATGCTAATGTTGGAAATGATTCTTCCTACTCGTTTGATCGCCTTCGGGAGGAGAACAATGCGATTATTTTAGCAGTAGGCGCAACGAAGCCAAGGGACCTTCCCGTACCTGGGCGAGAGCTGTCTGGAGTACATTTCGCTATGGAGTTTCTTCATGCAAACACCAAAAGCTTGCTTGATAGCAATCTCGAGAATGGTAACTATATTTCTGCCAAGGGTAAGAAGGTTGTGGTCATTGGTGGAGGTGACACTGGCACTGATTGTATTGGAACCTCTGTTCGCCATGGCTGTACTAACATTGTTAATTTGGAGCTTCTCCCTGAGCCACCACGAACAAGGGCACCAGGAAACCCTTGGCCACAGTGGCCTCGTATATTCCGAGTAGATTATGGGCATGCAGAAGTTGCAGCCAAGTTTGGCAAAGATCCAAGAACTTATGAGGTGCTGACTAAGAGGTTTGTGGGAGATGAGAATGGGGTTGTGAAGGGACTAGAGGTAGTACGTGTCAAATGGGAGAAGGATGCTACTGGCAAGTTCCAATTTAAGGAAATCGAGGGCTCTGAGGAGATAATTGAGGCCGATCTCGTCCTTCTAGCAATGGGGTTCCTTGGACCTGAAGCGGTGATTGCAGAGAAGTTGGGCTTGGAGCGTGACAATCGCTCAAACTTCAAGGCAGATTATGGTCGGTTCTCAACCAATGTGGATGGGGTCTTTGCTGCTGGGGATTGCCGGCGTGGTCAATCCTTGGTGGTGTGGGCAATCTCAGAGGGACGGCAAGCAGCTGCACAGGTTGACAACTACCTCTGCAAGGAAGAAGAAGACCACACAAACAGCAACGGGATCCCTGAATCCGATCTATTGAAGCGGCATCAAGAAATCAGCAAGAGCCATAAGACAGTTGCAAGCACACCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015930 GO:0016040 GO:0016053 GO:0016491 GO:0016638 GO:0016639 GO:0019676 GO:0019740 GO:0019752 GO:0032502 GO:0040007 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045181 GO:0046394 GO:0046686 GO:0048589 GO:0050896 GO:0055114 GO:0060359 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00250 ko00910 ko01100 ko01110 ko01120 ko01130 ko01230 map00250 map00910 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

Fer4_20 GATase_2 GXGXG Glu_syn_central Glu_synthase Pyr_redox_2

Protein Analysis

2209

Amino Acids

241.89

Weight (kDa)

6.21

Isoelectric Point (pI)

34.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	107 - 534	4.5e-184	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	584 - 872	9.4e-112	Glutamate synthase central domain
Glu_synthase	PF01645	939 - 1307	6.6e-150	Conserved region in glutamate synthase
GXGXG	PF01493	1391 - 1576	4.6e-88	GXGXG motif
Fer4_20	PF14691	1712 - 1823	6.7e-22	Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
Pyr_redox_2	PF07992	1836 - 2162	2.6e-27	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	1840 - 1875	1.9e-08	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0008640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53460 AT5G53460 AT5G53460
fragaria_vesca	FvH4_7g31770 FvH4_7g31770 FvH4_7g31770
malus_domestica	MD01G1219500.v1.1 MD07G1290500.v1.1
prunus_persica	Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1
pyrus_communis	pycom01g22930 pycom07g26320
rosa_chinensis	RchiOBHm_Chr1g0381001
rosa_laevigata	RLG00000026253
rosa_multiflora	Rmu_ssc0000032.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278870
rosa_rugosa	Rorug01G0426400
rosa_samantha	Rh1AG450100 Rh1BG406100 Rh1CG420100 Rh1DG436600
rosa_wichuraiana	Rw1G039220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	4433
AbsI	CCTCGAGG	1 cut(s)	853
Acc36I	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
AccB1I	GGYRCC	7 cut(s)	1663, 1744, 3548, 3739, 4460, 5476, 6055
AccB7I	CCANNNNNTGG	2 cut(s)	1328, 4090
AccBSI	CCGCTC	2 cut(s)	225, 283
AccII	CGCG	2 cut(s)	2187, 2320
AccIII	TCCGGA	2 cut(s)	45, 249
AclI	AACGTT	1 cut(s)	4228
AcsI	RAATTY	6 cut(s)	673, 2939, 3328, 4271, 4670, 4826
AcvI	CACGTG	2 cut(s)	1076, 2354
AdeI	CACNNNGTG	2 cut(s)	5247, 5378
AflII	CTTAAG	4 cut(s)	1088, 3416, 4739, 4976
AflIII	ACRYGT	2 cut(s)	3856, 6217
AhlI	ACTAGT	1 cut(s)	4466
AjuI	GAANNNNNNNTTGG	2 cut(s)	6341, 6373
AleI	CACNNNNGTG	1 cut(s)	2216
AloI	GAACNNNNNNTCC	4 cut(s)	438, 470, 5976, 6008
Alw21I	GWGCWC	5 cut(s)	1003, 1291, 1626, 2590, 4864
Alw26I	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5314, 5324, 5422
Alw44I	GTGCAC	2 cut(s)	2586, 4860
AlwNI	CAGNNNCTG	3 cut(s)	695, 2756, 4055
Ama87I	CYCGRG	5 cut(s)	284, 853, 2727, 5327, 5903
Aor13HI	TCCGGA	2 cut(s)	45, 249
ApaLI	GTGCAC	2 cut(s)	2586, 4860
ApoI	RAATTY	6 cut(s)	673, 2939, 3328, 4271, 4670, 4826
ArsI	GACNNNNNNTTYG	6 cut(s)	45, 77, 491, 523, 5441, 5473
AseI	ATTAAT	1 cut(s)	3404
Asp700I	GAANNNNTTC	5 cut(s)	152, 155, 775, 4245, 5734
AspLEI	GCGC	5 cut(s)	23, 753, 2100, 2316, 5798
AsuC2I	CCSGG	6 cut(s)	219, 299, 1401, 2742, 3270, 5078
AsuII	TTCGAA	1 cut(s)	3922
AsuNHI	GCTAGC	1 cut(s)	5542
AvaI	CYCGRG	5 cut(s)	284, 853, 2727, 5327, 5903
AvaII	GGWCC	9 cut(s)	48, 215, 247, 1979, 2039, 4081, 5174, 5812, 6326
AxyI	CCTNAGG	1 cut(s)	4874
BaeGI	GKGCMC	3 cut(s)	2590, 4864, 6058
BalI	TGGCCA	8 cut(s)	621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
BamHI	GGATCC	4 cut(s)	182, 1273, 5119, 6555
BanI	GGYRCC	7 cut(s)	1663, 1744, 3548, 3739, 4460, 5476, 6055
BanII	GRGCYC	3 cut(s)	1626, 3439, 6275
BauI	CACGAG	2 cut(s)	316, 2688
BbrPI	CACGTG	2 cut(s)	1076, 2354
BbsI	GAAGAC	4 cut(s)	1501, 2924, 3396, 6540
Bbv12I	GWGCWC	5 cut(s)	1003, 1291, 1626, 2590, 4864
BbvCI	CCTCAGC	2 cut(s)	75, 3885
BceAI	ACGGC	7 cut(s)	251, 345, 1573, 3753, 5498, 5687, 6503
BcgI	CGANNNNNNTGC	2 cut(s)	3107, 3141
BciVI	GTATCC	1 cut(s)	4404
BclI	TGATCA	3 cut(s)	4555, 4903, 5554
BcnI	CCSGG	6 cut(s)	219, 299, 1401, 2742, 3270, 5078
BcoDI	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5314, 5324, 5422
BcuI	ACTAGT	1 cut(s)	4466
BfaI	CTAG	8 cut(s)	1196, 2297, 2652, 3486, 4467, 5543, 6206, 6308
BfmI	CTRYAG	5 cut(s)	1483, 2418, 2438, 4035, 4137
BfoI	RGCGCY	3 cut(s)	24, 754, 2101
BfrI	CTTAAG	4 cut(s)	1088, 3416, 4739, 4976
BfuAI	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
BfuI	GTATCC	1 cut(s)	4404
BglI	GCCNNNNNGGC	3 cut(s)	286, 5539, 6079
BglII	AGATCT	2 cut(s)	916, 4702
BlpI	GCTNAGC	3 cut(s)	104, 1002, 2346
BmcAI	AGTACT	3 cut(s)	2510, 3825, 4797
Bme18I	GGWCC	9 cut(s)	48, 215, 247, 1979, 2039, 4081, 5174, 5812, 6326
BmeT110I	CYCGRG	5 cut(s)	284, 853, 2727, 5327, 5903
BmrI	ACTGGG	4 cut(s)	3024, 5043, 5253, 5511
BmtI	GCTAGC	1 cut(s)	5546
BmuI	ACTGGG	4 cut(s)	3024, 5043, 5253, 5511
BpiI	GAAGAC	4 cut(s)	1501, 2924, 3396, 6540
BplI	GAGNNNNNCTC	4 cut(s)	3107, 3139, 4293, 4325
BpmI	CTGGAG	6 cut(s)	668, 1455, 1701, 3538, 3641, 5862
Bpu10I	CCTNAGC	6 cut(s)	75, 3255, 3885, 4692, 4923, 6036
Bpu1102I	GCTNAGC	3 cut(s)	104, 1002, 2346
Bpu14I	TTCGAA	1 cut(s)	3922
BpuEI	CTTGAG	6 cut(s)	767, 1208, 1610, 2330, 4349, 5477
BpuMI	CCSGG	6 cut(s)	219, 299, 1401, 2742, 3270, 5078
BsaAI	YACGTR	3 cut(s)	1076, 2354, 6218
BsaBI	GATNNNNATC	2 cut(s)	3037, 5898
BsaI	GGTCTC	2 cut(s)	2150, 3602
BsaWI	WCCGGW	3 cut(s)	45, 249, 347
BsaXI	ACNNNNNCTCC	8 cut(s)	438, 468, 709, 739, 1909, 1939, 2782, 2812
Bse118I	RCCGGY	1 cut(s)	6444
Bse21I	CCTNAGG	1 cut(s)	4874
Bse3DI	GCAATG	8 cut(s)	931, 3027, 3066, 3312, 3994, 4186, 4354, 6318
Bse8I	GATNNNNATC	2 cut(s)	3037, 5898
BseAI	TCCGGA	2 cut(s)	45, 249
BseJI	GATNNNNATC	2 cut(s)	3037, 5898
BseMI	GCAATG	8 cut(s)	931, 3027, 3066, 3312, 3994, 4186, 4354, 6318
BseRI	GAGGAG	9 cut(s)	426, 593, 2168, 3181, 3902, 4895, 5315, 5780, 6293
BseSI	GKGCMC	3 cut(s)	2590, 4864, 6058
BseX3I	CGGCCG	1 cut(s)	220
BseYI	CCCAGC	7 cut(s)	523, 986, 990, 3578, 5368, 5537, 6434
BsgI	GTGCAG	7 cut(s)	179, 2412, 2769, 3735, 4041, 4305, 6483
Bsh1236I	CGCG	2 cut(s)	2187, 2320
Bsh1285I	CGRYCG	2 cut(s)	223, 1400
BshNI	GGYRCC	7 cut(s)	1663, 1744, 3548, 3739, 4460, 5476, 6055
BsiEI	CGRYCG	2 cut(s)	223, 1400
BsiHKAI	GWGCWC	5 cut(s)	1003, 1291, 1626, 2590, 4864
BsiHKCI	CYCGRG	5 cut(s)	284, 853, 2727, 5327, 5903
BslFI	GGGAC	9 cut(s)	175, 201, 1095, 3360, 4663, 5020, 5825, 6216, 6498
BsmAI	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5314, 5324, 5422
BsmBI	CGTCTC	1 cut(s)	2030
BsmFI	GGGAC	9 cut(s)	175, 201, 1095, 3360, 4663, 5020, 5825, 6216, 6498
BsmI	GAATGC	3 cut(s)	2538, 3427, 3580
Bso31I	GGTCTC	2 cut(s)	2150, 3602
BsoBI	CYCGRG	5 cut(s)	284, 853, 2727, 5327, 5903
Bsp119I	TTCGAA	1 cut(s)	3922
Bsp1286I	GDGCHC	8 cut(s)	1003, 1291, 1626, 2590, 3439, 4864, 6058, 6275
Bsp13I	TCCGGA	2 cut(s)	45, 249
Bsp1407I	TGTACA	2 cut(s)	759, 2372
Bsp1720I	GCTNAGC	3 cut(s)	104, 1002, 2346
Bsp19I	CCATGG	5 cut(s)	1322, 1732, 3587, 4084, 5998
BspEI	TCCGGA	2 cut(s)	45, 249
BspFNI	CGCG	2 cut(s)	2187, 2320
BspHI	TCATGA	4 cut(s)	1648, 3370, 3409, 4213
BspMAI	CTGCAG	3 cut(s)	1487, 2442, 4039
BspMI	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
BspOI	GCTAGC	1 cut(s)	5546
BspQI	GCTCTTC	1 cut(s)	4155
BspT104I	TTCGAA	1 cut(s)	3922
BspT107I	GGYRCC	7 cut(s)	1663, 1744, 3548, 3739, 4460, 5476, 6055
BspTI	CTTAAG	4 cut(s)	1088, 3416, 4739, 4976
BspTNI	GGTCTC	2 cut(s)	2150, 3602
BsrBI	CCGCTC	2 cut(s)	225, 283
BsrDI	GCAATG	8 cut(s)	931, 3027, 3066, 3312, 3994, 4186, 4354, 6318
BsrFI	RCCGGY	1 cut(s)	6444
BsrGI	TGTACA	2 cut(s)	759, 2372
BssAI	RCCGGY	1 cut(s)	6444
BssSI	CACGAG	2 cut(s)	316, 2688
Bst2BI	CACGAG	2 cut(s)	316, 2688
Bst6I	CTCTTC	6 cut(s)	3144, 3679, 3714, 4155, 4932, 5036
BstAFI	CTTAAG	4 cut(s)	1088, 3416, 4739, 4976
BstAPI	GCANNNNNTGC	1 cut(s)	6119
BstAUI	TGTACA	2 cut(s)	759, 2372
BstBAI	YACGTR	3 cut(s)	1076, 2354, 6218
BstBI	TTCGAA	1 cut(s)	3922
BstDSI	CCRYGG	7 cut(s)	1322, 1732, 2926, 3587, 4084, 5480, 5998
BstEII	GGTNACC	1 cut(s)	4219
BstENI	CCTNNNNNAGG	5 cut(s)	2164, 4877, 5379, 5762, 6521
BstFNI	CGCG	2 cut(s)	2187, 2320
BstH2I	RGCGCY	3 cut(s)	24, 754, 2101
BstHHI	GCGC	5 cut(s)	23, 753, 2100, 2316, 5798
BstMAI	GTCTC	9 cut(s)	618, 2030, 2150, 2428, 3303, 3602, 5314, 5324, 5422
BstMCI	CGRYCG	2 cut(s)	223, 1400
BstNSI	RCATGY	5 cut(s)	259, 420, 3509, 3860, 6113
BstPI	GGTNACC	1 cut(s)	4219
BstSFI	CTRYAG	5 cut(s)	1483, 2418, 2438, 4035, 4137
BstSLI	GKGCMC	3 cut(s)	2590, 4864, 6058
BstUI	CGCG	2 cut(s)	2187, 2320
BstV2I	GAAGAC	4 cut(s)	1501, 2924, 3396, 6540
BstXI	CCANNNNNNTGG	2 cut(s)	3665, 4298
BstZI	CGGCCG	1 cut(s)	220
Bsu36I	CCTNAGG	1 cut(s)	4874
BsuI	GTATCC	1 cut(s)	4404
BtgI	CCRYGG	7 cut(s)	1322, 1732, 2926, 3587, 4084, 5480, 5998
BtgZI	GCGATG	1 cut(s)	317
BtsI	GCAGTG	2 cut(s)	117, 3333
BveI	ACCTGC	4 cut(s)	2431, 2992, 3172, 4039
CaiI	CAGNNNCTG	3 cut(s)	695, 2756, 4055
CciI	TCATGA	4 cut(s)	1648, 3370, 3409, 4213
CfoI	GCGC	5 cut(s)	23, 753, 2100, 2316, 5798
Cfr10I	RCCGGY	1 cut(s)	6444
CseI	GACGC	3 cut(s)	1368, 3436, 4754
CsiI	ACCWGGT	1 cut(s)	5170
CspCI	CAANNNNNGTGG	6 cut(s)	2116, 2151, 2908, 2943, 6396, 6431
DraI	TTTAAA	3 cut(s)	2944, 3930, 5001
DraIII	CACNNNGTG	2 cut(s)	5247, 5378
DrdI	GACNNNNNNGTC	1 cut(s)	4433
DseDI	GACNNNNNNGTC	1 cut(s)	4433
EagI	CGGCCG	1 cut(s)	220
Eam1104I	CTCTTC	6 cut(s)	3144, 3679, 3714, 4155, 4932, 5036
EarI	CTCTTC	6 cut(s)	3144, 3679, 3714, 4155, 4932, 5036
EciI	GGCGGA	1 cut(s)	353
Ecl136II	GAGCTC	1 cut(s)	1624
EclXI	CGGCCG	1 cut(s)	220
Eco24I	GRGCYC	3 cut(s)	1626, 3439, 6275
Eco31I	GGTCTC	2 cut(s)	2150, 3602
Eco32I	GATATC	2 cut(s)	2562, 3376
Eco47I	GGWCC	9 cut(s)	48, 215, 247, 1979, 2039, 4081, 5174, 5812, 6326
Eco52I	CGGCCG	1 cut(s)	220
Eco53kI	GAGCTC	1 cut(s)	1624
Eco72I	CACGTG	2 cut(s)	1076, 2354
Eco81I	CCTNAGG	1 cut(s)	4874
Eco88I	CYCGRG	5 cut(s)	284, 853, 2727, 5327, 5903
Eco91I	GGTNACC	1 cut(s)	4219
EcoICRI	GAGCTC	1 cut(s)	1624
EcoNI	CCTNNNNNAGG	5 cut(s)	2164, 4877, 5379, 5762, 6521
EcoO109I	RGGNCCY	2 cut(s)	2039, 5812
EcoO65I	GGTNACC	1 cut(s)	4219
EcoRI	GAATTC	2 cut(s)	673, 3328
EcoRV	GATATC	2 cut(s)	2562, 3376
EcoT22I	ATGCAT	1 cut(s)	4966
EcoT38I	GRGCYC	3 cut(s)	1626, 3439, 6275
Esp3I	CGTCTC	1 cut(s)	2030
FaqI	GGGAC	9 cut(s)	175, 201, 1095, 3360, 4663, 5020, 5825, 6216, 6498
FauI	CCCGC	2 cut(s)	231, 5667
FauNDI	CATATG	1 cut(s)	839
FbaI	TGATCA	3 cut(s)	4555, 4903, 5554
FriOI	GRGCYC	3 cut(s)	1626, 3439, 6275
FspBI	CTAG	8 cut(s)	1196, 2297, 2652, 3486, 4467, 5543, 6206, 6308
GlaI	GCGC	5 cut(s)	22, 752, 2099, 2315, 5797
GsaI	CCCAGC	7 cut(s)	527, 990, 994, 3582, 5372, 5541, 6438
GsuI	CTGGAG	6 cut(s)	668, 1455, 1701, 3538, 3641, 5862
HaeII	RGCGCY	3 cut(s)	24, 754, 2101
HgaI	GACGC	3 cut(s)	1368, 3436, 4754
HhaI	GCGC	5 cut(s)	23, 753, 2100, 2316, 5798
Hin6I	GCGC	5 cut(s)	21, 751, 2098, 2314, 5796
HinP1I	GCGC	5 cut(s)	21, 751, 2098, 2314, 5796
HincII	GTYRAC	5 cut(s)	1060, 3967, 5680, 5704, 6508
HindII	GTYRAC	5 cut(s)	1060, 3967, 5680, 5704, 6508
HindIII	AAGCTT	5 cut(s)	1877, 2601, 2837, 4777, 5884
HpaI	GTTAAC	2 cut(s)	1060, 5680
Hpy99I	CGWCG	1 cut(s)	215
HpyCH4IV	ACGT	9 cut(s)	1075, 1413, 2134, 2353, 4228, 4356, 4650, 5200, 6217
HpySE526I	ACGT	9 cut(s)	1075, 1413, 2134, 2353, 4228, 4356, 4650, 5200, 6217
HspAI	GCGC	5 cut(s)	21, 751, 2098, 2314, 5796
Kpn2I	TCCGGA	2 cut(s)	45, 249
KroI	GCCGGC	1 cut(s)	6444
KroNI	GCCGGC	1 cut(s)	6446
Ksp22I	TGATCA	3 cut(s)	4555, 4903, 5554
KspAI	GTTAAC	2 cut(s)	1060, 5680
LguI	GCTCTTC	1 cut(s)	4155
MabI	ACCWGGT	1 cut(s)	5170
MaeI	CTAG	8 cut(s)	1196, 2297, 2652, 3486, 4467, 5543, 6206, 6308
MaeII	ACGT	9 cut(s)	1075, 1413, 2134, 2353, 4228, 4356, 4650, 5200, 6217
MbiI	CCGCTC	2 cut(s)	225, 283
MfeI	CAATTG	3 cut(s)	1140, 1929, 3384
MhlI	GDGCHC	8 cut(s)	1003, 1291, 1626, 2590, 3439, 4864, 6058, 6275
MlsI	TGGCCA	8 cut(s)	621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
MluNI	TGGCCA	8 cut(s)	621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
MlyI	GAGTC	7 cut(s)	308, 577, 620, 1386, 1737, 4729, 5316
Mox20I	TGGCCA	8 cut(s)	621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
Mph1103I	ATGCAT	1 cut(s)	4966
MroI	TCCGGA	2 cut(s)	45, 249
MroNI	GCCGGC	1 cut(s)	6444
MroXI	GAANNNNTTC	5 cut(s)	152, 155, 775, 4245, 5734
MscI	TGGCCA	8 cut(s)	621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
MslI	CAYNNNNRTG	9 cut(s)	421, 819, 1961, 2216, 2706, 2814, 3071, 3767, 4563
Msp20I	TGGCCA	8 cut(s)	621, 1023, 1442, 3065, 3669, 3695, 4787, 6073
MspA1I	CMGCKG	5 cut(s)	523, 880, 895, 990, 6497
MspCI	CTTAAG	4 cut(s)	1088, 3416, 4739, 4976
MunI	CAATTG	3 cut(s)	1140, 1929, 3384
Mva1269I	GAATGC	3 cut(s)	2538, 3427, 3580
MvnI	CGCG	2 cut(s)	2187, 2320
NaeI	GCCGGC	1 cut(s)	6446
NciI	CCSGG	6 cut(s)	219, 299, 1401, 2742, 3270, 5078
NcoI	CCATGG	5 cut(s)	1322, 1732, 3587, 4084, 5998
NdeI	CATATG	1 cut(s)	839
NgoMIV	GCCGGC	1 cut(s)	6444
NheI	GCTAGC	1 cut(s)	5542
NmeAIII	GCCGAG	2 cut(s)	634, 5716
NmuCI	GTSAC	9 cut(s)	1414, 1954, 3293, 3906, 4219, 4340, 5581, 5960, 6365
NsiI	ATGCAT	1 cut(s)	4966
NspI	RCATGY	5 cut(s)	259, 420, 3509, 3860, 6113
NspV	TTCGAA	1 cut(s)	3922
OliI	CACNNNNGTG	1 cut(s)	2216
PaeI	GCATGC	2 cut(s)	3509, 6113
PaeR7I	CTCGAG	3 cut(s)	853, 5327, 5903
PagI	TCATGA	4 cut(s)	1648, 3370, 3409, 4213
PasI	CCCWGGG	1 cut(s)	3431
PciI	ACATGT	1 cut(s)	3856
PciSI	GCTCTTC	1 cut(s)	4155
PcsI	WCGNNNNNNNCGW	3 cut(s)	95, 291, 6091
PctI	GAATGC	3 cut(s)	2538, 3427, 3580
PdiI	GCCGGC	1 cut(s)	6446
PdmI	GAANNNNTTC	5 cut(s)	152, 155, 775, 4245, 5734
PflFI	GACNNNGTC	1 cut(s)	1401
PflMI	CCANNNNNTGG	2 cut(s)	1328, 4090
PleI	GAGTC	7 cut(s)	308, 576, 619, 1386, 1736, 4729, 5316
PmaCI	CACGTG	2 cut(s)	1076, 2354
PmlI	CACGTG	2 cut(s)	1076, 2354
PpsI	GAGTC	7 cut(s)	308, 576, 619, 1386, 1736, 4729, 5316
Ppu21I	YACGTR	3 cut(s)	1076, 2354, 6218
PpuMI	RGGWCCY	2 cut(s)	2039, 5812
PscI	ACATGT	1 cut(s)	3856
PshBI	ATTAAT	1 cut(s)	3404
Psp124BI	GAGCTC	1 cut(s)	1626
Psp1406I	AACGTT	1 cut(s)	4228
Psp5II	RGGWCCY	2 cut(s)	2039, 5812
PspCI	CACGTG	2 cut(s)	1076, 2354
PspEI	GGTNACC	1 cut(s)	4219
PspFI	CCCAGC	7 cut(s)	523, 986, 990, 3578, 5368, 5537, 6434
PspPPI	RGGWCCY	2 cut(s)	2039, 5812
PspXI	VCTCGAGB	1 cut(s)	853
PstI	CTGCAG	3 cut(s)	1487, 2442, 4039
PstNI	CAGNNNCTG	3 cut(s)	695, 2756, 4055
PsyI	GACNNNGTC	1 cut(s)	1401
PvuII	CAGCTG	4 cut(s)	880, 895, 990, 6497
RseI	CAYNNNNRTG	9 cut(s)	421, 819, 1961, 2216, 2706, 2814, 3071, 3767, 4563
SacI	GAGCTC	1 cut(s)	1626
SapI	GCTCTTC	1 cut(s)	4155
ScaI	AGTACT	3 cut(s)	2510, 3825, 4797
SchI	GAGTC	7 cut(s)	308, 577, 620, 1386, 1737, 4729, 5316
SduI	GDGCHC	8 cut(s)	1003, 1291, 1626, 2590, 3439, 4864, 6058, 6275
SexAI	ACCWGGT	1 cut(s)	5170
SfcI	CTRYAG	5 cut(s)	1483, 2418, 2438, 4035, 4137
SfiI	GGCCNNNNNGGCC	1 cut(s)	6079
Sfr274I	CTCGAG	3 cut(s)	853, 5327, 5903
SfuI	TTCGAA	1 cut(s)	3922
SinI	GGWCC	9 cut(s)	48, 215, 247, 1979, 2039, 4081, 5174, 5812, 6326
SlaI	CTCGAG	3 cut(s)	853, 5327, 5903
SmiMI	CAYNNNNRTG	9 cut(s)	421, 819, 1961, 2216, 2706, 2814, 3071, 3767, 4563
SpeI	ACTAGT	1 cut(s)	4466
SphI	GCATGC	2 cut(s)	3509, 6113
SspI	AATATT	3 cut(s)	1301, 1574, 4064
SspMI	CTAG	8 cut(s)	1196, 2297, 2652, 3486, 4467, 5543, 6206, 6308
SstI	GAGCTC	1 cut(s)	1626
TaiI	ACGT	9 cut(s)	1078, 1416, 2137, 2356, 4231, 4359, 4653, 5203, 6220
TaqII	GACCGA	1 cut(s)	1967
TauI	GCSGC	8 cut(s)	11, 179, 225, 283, 2873, 3648, 3704, 6583
TseFI	GTSAC	9 cut(s)	1414, 1954, 3293, 3906, 4219, 4340, 5581, 5960, 6365
Tsp45I	GTSAC	9 cut(s)	1414, 1954, 3293, 3906, 4219, 4340, 5581, 5960, 6365
TspGWI	ACGGA	5 cut(s)	22, 49, 389, 782, 2915
Tth111I	GACNNNGTC	1 cut(s)	1401
Van91I	CCANNNNNTGG	2 cut(s)	1328, 4090
Vha464I	CTTAAG	4 cut(s)	1088, 3416, 4739, 4976
VneI	GTGCAC	2 cut(s)	2586, 4860
VpaK11BI	GGWCC	9 cut(s)	48, 215, 247, 1979, 2039, 4081, 5174, 5812, 6326
VspI	ATTAAT	1 cut(s)	3404
XagI	CCTNNNNNAGG	5 cut(s)	2164, 4877, 5379, 5762, 6521
XapI	RAATTY	6 cut(s)	673, 2939, 3328, 4271, 4670, 4826
XceI	RCATGY	5 cut(s)	259, 420, 3509, 3860, 6113
XcmI	CCANNNNNNNNNTGG	5 cut(s)	629, 2494, 3340, 4795, 6066
XhoI	CTCGAG	3 cut(s)	853, 5327, 5903
XmnI	GAANNNNTTC	5 cut(s)	152, 155, 775, 4245, 5734
XspI	CTAG	8 cut(s)	1196, 2297, 2652, 3486, 4467, 5543, 6206, 6308
ZrmI	AGTACT	3 cut(s)	2510, 3825, 4797
Zsp2I	ATGCAT	1 cut(s)	4966

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.