Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0008640

Physical Location & Seq

Genomic Coordinates Reverse (-)

1649403 .. 1658892

Gene Structure 9490 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000026253

Sequence Viewer

CDS Protein Promoter (2k)

Length: 6633 bp

ATGTTGGCGGCGAGTTCCGGCGGTGGTGGTTCCGTTCTCCAACTCCGGACCAAACCTTCCGTCCTCGCGTCACCTCAGCTCAATGCCTCGCCGATAGCGAGGCTAAGCACCAGAGCAGCCACCGCTCGCGCTGGTTCCAAAGCTATTGCGAAGAAGTTCTTCGGGACGAGATTGAGGGCCGCCGGATCCGAGAGGCTGCACTTATGGCGATCCGACGGTCCCGGCCGCTCGCCGAAGCTGCGGGTGGTGGTCCGGAACATGCTCTCCGCCGTGCCGGAGAAGCCGCTCGGGCTATACGACCCGGCGATGGATAAGGACTCGTGCGGTGTCGGCTTCGTGGCGGAATTGTCCGGTGAAAGCAGCCGTAAAACGATTACGGATGCTTTGGAAATGTTGGTGCGGATGACACATAGAGGAGCTTGTGGATGTGAAACAAACACTGGTGATGGAGCTGGTGTTCTTGTGGCGATTCCTCACGATTTCTACAAGAAGGCTGCCAAAGATAATGGGTTTGAGCTTCCACCGCTGGGTGAGTATGCTGTTGGTATGCTATACTTGCCTACATCGGAGAGTCGTAGAGAGGAAAGCAAAAATGTGTTTACAAAGGTTGCCGAGTCTCTTGGCCATACTGTTCTTGGGTGGCGATCAGTTCCCACTGATAACTCAGATTTGGGGAATTCTGCTCTCCAGACAGAACCTGTTATTGAGCAAGTGTTCCTTACTCCAACTCCGAGGTCGAAAGTTGATCTTGAGCGCCAGATGTACATACTTAGAAGGGTTTCAATGGTTGCCATCCGTGCTGCATTAAACCTTCAACATGGTGGTGCTAAAGATTTCTACATATGTTCACTTTCCTCGAGGACGATTGTTTACAAGGGTCAGCTGAAACCCGAACAACTGAAGGGTTACTATTATGCAGATCTTGGCAATGAAAGGTTTACGAGCTACATGGCCCTGGTACATTCTCGATTCTCAACAAATACTTTCCCCAGCTGGGACCGTGCTCAGCCCATGCGTGTCATTGGCCACAATGGAGAAATCAACACCCTTAGAGGAAATGTTAACTGGATGAAGGCACGTGAGGGACTGCTCAAGTGTACTGAACTTGGTTTATCAAAAAATGAGTTAAAGAAGCTGTTACCAATTGTGGATGCCAGTTCATCCGATTCAGGAGCTTTTGATGGTGTCCTTGAGCTTCTAGTTCGAGCTGGCAGAAGCCTCCCTGAAGCTATTATGATGATGATTCCTGAAGCATGGCAAAATGACAAGAACATGGATCCTGATAGGAGAGCACTGTATGAATATTTCTCATCTCTCATGGAGCCATGGGATGGGCCAGCTCTAATATCATTTACTGATGGACGCTATCTGGGAGCTACATTGGATCGGAATGGACTCCGACCGGGTCGCTTTTATGTCACCCACAGTGGACGAGTTATTATGGCCAGTGAAGTTGGGGTTGTAGACGTACCTCCAGAAGATGTCTGCAGGAAAGGAAGACTTAACCCTGGAATGATGCTTTTGGTGGATTTTGAGAATCATATAGTGGTAGATGATGAAGCCTTGAAGCAGCAATATTCACTGGCAAGGCCGTATGGGGAGTGGCTGAAAAGGCAGAAGATTGAGCTCAAGGACATAGTTGATTCTGTTCATGAATCTGACAGGGTCCCTCCATCCATAGCTGGAGTCGTTCCTGCATCTACTGATGATGACAACATGGAAAACATGGGAGTCCATGGTTTACTGGCACCACTGAAGGCTTTTGGTTACACAGTTGAAGCCTTGGAAATGTTGTTGCTTCCTATGGCAAAAGATGGCGTAGAGGCTCTTGGGTCAATGGGAAATGATACCCCCTTGGCTGTTATGTCCAATAGGGAAAAGCTTACATTTGAGTATTTCAAGCAAATGTTCGCCCAAGTAACAAACCCTCCAATTGATCCAATTCGAGAAAAGGTAGTCACGTCTATGGAATGTATGATCGGTCCAGAAGGTGATCTAACAGAAACCACTGAAGAACAATGCCATCGTCTCTCACTCAAAGGTCCTCTTTTAAGCATTGAGGAAATGGAGGCGATCAAAAAAATGAATTACAGAGGTTGGTGCTGTAAGGTTCTTGACATAACTTACTCCAAGGAACGTGGTAGAAAGGGATTGGAGGAGACCTTGGATAGGATCTGTGCTGAAGCACGCGAGGCAATTAAAAAGGGATATACCACACTGGTGCTTTCAGACAGAGCCTTCTCCCCAAAACGTGTTGCTGTAAGCTCACTCTTGGCTGTTGGTGCTGTTCATCAACATCTAGTTAAAAATCTTGAGCGCACGCGAGTAGGGTTGATAATTGAATCTGCTGAGCCGCGTGAGGTACACCATTTCTGTACGTTAGTTGGATTCGGTGCAGATGCCATCTGCCCCTACCTGGCTGTAGAGGCTATTTGGAGACTGCATGTTGATGGAAAGATCCCACCAAAATCTAATGGTACAATATATTCCAAGGATGAACTCGTTAAAAAGTACTTCAAAGCAAGCAACTATGGAATGCAGAAGGTTCTTGCCAAGATGGGGATATCTACTTTGGCCTCTTACAAGGGTGCACAGATTTTTGAAGCTTTGGGTCTTTCATCTGAAGTGATTGAGAGGTGCTTTGCAGGAACTCCTAGTAGAGTTGAGGGGGCAACATTTGAGATGCTTGCTCGTGATGGACTTCATCTGCATGATTTGGCATTTCCCTCTCGGGCTTTTCCTCCGGGTAGTGCAGAAGCTGTGGCACTACCAAATCCAGGGGATTATCATTGGAGAAAAGGTGGTGAAGTTCACCTAAATGATCCTTTTGCTATTTCCAAGCTTCAGGAGGCTGCCAGAACTAACAGTGTGGCGGCATATAAAGAATACTCCAAGCTCATTCATCAATTAAATAAAGCCTGCAATCTCCGTGGTCTTCTGAAATTTAAAAACACAGAGCAGCAAATTCACTTGGATGAAGTGGAACCTGCCAGTGAGATTGTTAAACGGTTCTGTACTGGGGCAATGAGTTATGGATCAATATCATTGGAGGCACACACTACACTGGCCATTGCTATGAACAGAATGGGAGGAAAGTCGAATACAGGAGAGGGAGGTGAGCAGCCCTCTCGTATGGAGCCTCTTCCTGATGGTTCAAGGAATCCAAAGAGGAGTGCAATTAAGCAGGTTGCAAGTGGGAGATTTGGAGTTTCAAGTTACTATCTTACAAATGCTGATGAACTCCAAATAAAAATGGCTCAGGGGGCCAAGCCCGGTGAAGGAGGTGAGCTTCCTGGTCACAAGGTTATTGGAGACATTGCTGTTACCAGGAATTCCACTGCTGGTGTGGGACTTATCAGCCCACCTCCCCATCATGATATCTACTCAATTGAAGACCTTGCTCAATTAATTCATGATCTTAAGAACGCTAACCCAGGGGCTCGAATCAGCGTCAAGTTAGTGTCTGAAGCTGGTGTAGGAGTAGTTGCTAGTGGAGTTGTGAAGGGGCATGCTGACCATGTTTTAATTGCCGGACATGATGGAGGCACTGGGGCCTCCAGATGGACCGGCATTAAGAATGCTGGGCTTCCATGGGAACTTGGCTTGGCTGAGACCCATCAAACCCTGGTTGCTAATGATCTTCGTGGCCGCACAGTTCTCCAGACAGATGGCCAACTAAAAACTGGAAGAGATGTGGCCATAGCCGCACTTCTCGGTGCAGAAGAGTTTGGGTTCAGCACGGCACCCCTCATAACACTTGGCTGCATTATGATGCGGAAGTGCCACAAGAACACCTGTCCTGTCGGAATTGCAACGCAAGATCCAGTACTTCGTGAGAAGTTTGCCGGAGAACCTGAACATGTTATCAACTTTTTCTTCATGGTAGCTGAGGAGGTGAGAGAGATAATGTCACAACTTGGTTTTCGAACTTTAAATGAAATGGTTGGTCGTTCGGATATGCTTGAAGTCGACAAAGAAGTTACTAAAGGCAATGAGAAGCTGGACAACATTGATCTTTCCCTGTTACTTAGACCTGCTGCAGACATTCGTCCAGAAGCTGCACAATATTGTGTTCAGAAACAGGACCATGGGTTGGACATGGCTCTTGACCACAAACTTATTTCACTGTCCAGTTCTGCTATAGAGAAGGCTCTTCCTGCATACTTTGAAACACCGATTTGCAATGTGAATCGTGCTGTTGGAACAATGCTTAGTCATGAGGTGACCAAACGTTACAACAGGGAAGGGCTTCCAGCTGACACCATTCATATCAAATTCAACGGAAGTGCAGGCCAGAGCCTTGGAGCTTTCCTCTGCCCTGGCATCACGCTTGAGCTTGAAGGTGACAGCAATGATTATGTTGGTAAAGGATTGTCTGGTGGCAAGATCATAGTTTATCCCCCCAAGGGAAGCAAGTTTGACCCAAAAGAAAACATTGTCATTGGTAATGTAGCTCTTTATGGTGCCACTAGTGGGGAGGCATATTTCAATGGAATGGCAGCAGAAAGATTCTGTGTTCGCAATTCAGGGGCAAGGGCAGTAGTTGAAGGTGTCGGTGATCATGGCTGTGAGTACATGACCGGTGGCACTGTTGTTGTGCTTGGGAAAACTGGCAGGAACTTTGCTGCTGGTATGAGTGGTGGCATAGCCTACGTTCTTGATGTGGATGGAAAATTTTTGTCCCGATGCAATCCTGAGCTTGTAGATCTTGATAAAGTTGAAGGAGAAGATAGTATGACTCTTAAAATGATGATACAGCAGCATCAGCGTCATACAAAAAGCTTGCTGGCCAGTGAAGTACTGGCTGATTTTGAGAATCTTTTGCCTAAATTCATCAAGGTTATCCCCAGAGAGTACAAGAGTGCACTAGCAAACCTGAGGGAGGAGGCCTCCAAACAGGCCGTTGATCAAGCTGATGAAGAAGCTCCGGAGCAAGAGGAAGAGGTGGAAATAAAGGAGAAAGATGCATTTGAAGAACTTAAGAAGATGGCATCTGCATCTTTAAATGGGACATCTAATCAGGTAGAAGATGCTGAAACATTGAAGAGGCCTAGTGAGGTCAGCAAAGCTGTTAAACACCGGGGTTTCATTTCTTATGAGCGTGAAGGCGTCCAATACAGGGATCCCAAAGTTCGGATGAATGACTGGAAGGAAGTTATGGAGGAAACTAAACCTGGTCCACTTGTGAATACGCAGTCAGCGCGTTGCATGGACTGTGGCACTCCTTTCTGCCATCAGGAAAACACTGGGTGTCCTCTTGGTAACAAAATACCTGAGTTCAATGAATTGGTGTACCAGAATAGGTGGCATGAAGCATTAGAGAGACTCCTCGAGACAAATAACTTCCCTGAGTTTACTGGTCGAGTTTGCCCAGCACCTTGTGAAGGTTCATGTGTTCTGGGTATTATTGAGAATCCTGTCTCTATTAAAAGCATTGAATGTGCCATCATTGACAAGGCTTTTGAGGAAGGTTGGATGGTGCCACGGCCTCCACTCAAGAGAACTGGGAAAAAGGTTGCCATTGTCGGAAGTGGGCCTGCTGGGCTAGCTGCTGCTGATCAACTAAACAGAATAGGTCATACAGTGACAGTTTACGAGCGTGCTGATAGAATTGGAGGGCTGATGATGTATGGAGTGCCTAATATGAAGGCTGACAAAGTTGATATAGTTCAACGGCGGGTTAACCTTATGGCTGAGGAAGGTGTCAACTTTGTGGTCAATGCTAATGTTGGAAATGATCCTTCCTACTCGTTTGATCGCCTTCGGGAGGAGAACAATGCGATTATTTTAGCTGTAGGCGCAACGAAGCCAAGGGACCTTCCCGTACCTGGGCGAGAGCTGTCAGGAGTACATTTTGCTATGGAGTTTCTTCATGCAAACACCAGAAGCTTGCTTGATAGCAATCTCGAGAATGGTAACTACATCTCTGCCAAGGGTAAGAAGGTTGTGGTCATTGGTGGAGGTGACACTGGCACTGATTGTATTGGAACCTCTGTTCGCCATGGCTGTACTAATGTCGTAAATTTGGAGCTTCTCCCTGAGCCACCACGAACAAGGGCACCAGGAAACCCTTGGCCACAGTGGCCTCGTATATTCCGAGTAGATTATGGGCACGCAGAAGTTGCAGCCAAGTTTGGCAAAGATCCAAGAACTTATGAGGTGCTGACTAAGAGGTTTGTGGGAGATGAGAATGGCGTTGTGAAGGGACTTGAGGTAGTACGTGTCAAATGGGAGAAGGATGCTACTGGCAAGTTCCAATTTAAGGAAATCGAGGGCTCTGAGGAGACAATTGAGGCTGATCTTGTCCTTCTAGCAATGGGGTTCCTTGGACCCGAGGCGACGATTGCAGAGAAGTTGGGTTTGGAGCGTGACAATCGATCAAACTTCAAGGCAGATTATGGTCGGTTCTCAACCAATGTGGATGGGGTCTTCGCTGCTGGGGACTGCCGGCGTGGTCAATCCCTGGTGGTGTGGGCAATCTCAGAGGGACGGCAAGCAGCTGCACAGGTTGACAACTACCTCTGCAAGGAAGAAGAAGACTACACAAACAGCAACGGGAGTCCTGAATCCGATCTCTTGAAGCGGCATCAAGAAATCAGCAAGAGCCATAAGACAGTTGCAAGCACACCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006536 GO:0006537 GO:0006541 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0015930 GO:0016040 GO:0016053 GO:0016491 GO:0016638 GO:0016639 GO:0019676 GO:0019740 GO:0019752 GO:0032502 GO:0040007 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043648 GO:0043650 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045181 GO:0046394 GO:0046686 GO:0048589 GO:0050896 GO:0055114 GO:0060359 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko00250 ko00910 ko01100 ko01110 ko01120 ko01130 ko01230 map00250 map00910 map01100 map01110 map01120 map01130 map01230

Pfam Domains

Protein Families

Fer4_20 GATase_2 GXGXG Glu_syn_central Glu_synthase Pyr_redox_2

Protein Analysis

2211

Amino Acids

241.98

Weight (kDa)

6.25

Isoelectric Point (pI)

34.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GATase_2	PF00310	108 - 535	3.8e-184	Glutamine amidotransferases class-II
Glu_syn_central	PF04898	585 - 873	7.9e-112	Glutamate synthase central domain
Glu_synthase	PF01645	940 - 1308	5.3e-150	Conserved region in glutamate synthase
GXGXG	PF01493	1392 - 1577	7.6e-89	GXGXG motif
Fer4_20	PF14691	1713 - 1824	6.7e-22	Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster
Pyr_redox_2	PF07992	1837 - 2163	1.4e-27	Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8	PF13450	1841 - 1876	1.9e-08	NAD(P)-binding Rossmann-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0008640)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G53460 AT5G53460 AT5G53460
fragaria_vesca	FvH4_7g31770 FvH4_7g31770 FvH4_7g31770
malus_domestica	MD01G1219500.v1.1 MD07G1290500.v1.1
prunus_persica	Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1 Prupe.2G311700_v2.0.a1
pyrus_communis	pycom01g22930 pycom07g26320
rosa_chinensis	RchiOBHm_Chr1g0381001
rosa_laevigata	RLG00000026253
rosa_multiflora	Rmu_ssc0000032.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278870
rosa_rugosa	Rorug01G0426400
rosa_samantha	Rh1AG450100 Rh1BG406100 Rh1CG420100 Rh1DG436600
rosa_wichuraiana	Rw1G039220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	856
Acc36I	ACCTGC	3 cut(s)	2995, 3175, 4042
AccB1I	GGYRCC	5 cut(s)	1747, 3742, 4463, 5479, 6058
AccB7I	CCANNNNNTGG	2 cut(s)	1331, 4093
AccBSI	CCGCTC	3 cut(s)	125, 228, 286
AccI	GTMKAC	2 cut(s)	1464, 3969
AccII	CGCG	6 cut(s)	68, 129, 2190, 2323, 2356, 5203
AccIII	TCCGGA	3 cut(s)	45, 252, 4927
AclI	AACGTT	1 cut(s)	4231
AcsI	RAATTY	8 cut(s)	676, 2942, 2964, 3331, 4274, 4673, 4829, 6022
AcuI	CTGAAG	9 cut(s)	920, 1245, 1269, 1775, 2031, 2202, 2643, 2828, 3486
AcvI	CACGTG	1 cut(s)	1079
AcyI	GRCGYC	1 cut(s)	5109
AdeI	CACNNNGTG	2 cut(s)	5250, 5381
AflII	CTTAAG	2 cut(s)	3419, 4979
AflIII	ACRYGT	3 cut(s)	2251, 3859, 6220
AgeI	ACCGGT	1 cut(s)	4580
AhlI	ACTAGT	1 cut(s)	4469
AjiI	CACGTC	1 cut(s)	1962
AjuI	GAANNNNNNNTTGG	2 cut(s)	6344, 6376
AleI	CACNNNNGTG	1 cut(s)	2219
AloI	GAACNNNNNNTCC	6 cut(s)	248, 280, 441, 473, 5979, 6011
Alw21I	GWGCWC	5 cut(s)	1006, 1294, 1629, 2593, 4867
Alw44I	GTGCAC	2 cut(s)	2589, 4863
AlwNI	CAGNNNCTG	3 cut(s)	698, 2759, 4058
Ama87I	CYCGRG	6 cut(s)	287, 856, 2730, 5330, 5906, 6332
Aor13HI	TCCGGA	3 cut(s)	45, 252, 4927
ApaLI	GTGCAC	2 cut(s)	2589, 4863
ApoI	RAATTY	8 cut(s)	676, 2942, 2964, 3331, 4274, 4673, 4829, 6022
ArsI	GACNNNNNNTTYG	4 cut(s)	45, 77, 5444, 5476
AseI	ATTAAT	1 cut(s)	3407
AsiGI	ACCGGT	1 cut(s)	4580
Asp700I	GAANNNNTTC	4 cut(s)	155, 158, 778, 4248
AspLEI	GCGC	5 cut(s)	131, 756, 2319, 5203, 5801
AsuC2I	CCSGG	6 cut(s)	222, 302, 1404, 2745, 3273, 5081
AsuII	TTCGAA	1 cut(s)	3925
AsuNHI	GCTAGC	1 cut(s)	5545
AvaI	CYCGRG	6 cut(s)	287, 856, 2730, 5330, 5906, 6332
AxyI	CCTNAGG	1 cut(s)	4877
BaeGI	GKGCMC	4 cut(s)	2593, 4867, 6061, 6114
BalI	TGGCCA	8 cut(s)	624, 1026, 1445, 3068, 3672, 3698, 4790, 6076
BamHI	GGATCC	3 cut(s)	185, 1276, 5122
BanI	GGYRCC	5 cut(s)	1747, 3742, 4463, 5479, 6058
BanII	GRGCYC	3 cut(s)	1629, 3442, 6278
BauI	CACGAG	2 cut(s)	319, 2691
BbrPI	CACGTG	1 cut(s)	1079
BbsI	GAAGAC	5 cut(s)	1504, 2927, 3399, 6421, 6543
Bbv12I	GWGCWC	5 cut(s)	1006, 1294, 1629, 2593, 4867
BbvCI	CCTCAGC	3 cut(s)	75, 3888, 5694
BceAI	ACGGC	8 cut(s)	254, 348, 1576, 3756, 4886, 5501, 5690, 6506
BcgI	CGANNNNNNTGC	2 cut(s)	3110, 3144
BclI	TGATCA	3 cut(s)	4558, 4906, 5557
BcnI	CCSGG	6 cut(s)	222, 302, 1404, 2745, 3273, 5081
BcuI	ACTAGT	1 cut(s)	4469
BfaI	CTAG	9 cut(s)	1199, 2300, 2655, 3489, 4470, 4868, 5052, 5546, 6311
BfmI	CTRYAG	5 cut(s)	1486, 2421, 4038, 4140, 5793
BfoI	RGCGCY	1 cut(s)	757
BfrI	CTTAAG	2 cut(s)	3419, 4979
BfuAI	ACCTGC	3 cut(s)	2995, 3175, 4042
BglI	GCCNNNNNGGC	3 cut(s)	289, 5542, 6082
BglII	AGATCT	2 cut(s)	919, 4705
BlpI	GCTNAGC	3 cut(s)	104, 1005, 2349
BmcAI	AGTACT	3 cut(s)	2513, 3828, 4800
BmeT110I	CYCGRG	6 cut(s)	287, 856, 2730, 5330, 5906, 6332
BmgBI	CACGTC	1 cut(s)	1962
BmrI	ACTGGG	4 cut(s)	3027, 3558, 5256, 5514
BmtI	GCTAGC	1 cut(s)	5549
BmuI	ACTGGG	4 cut(s)	3027, 3558, 5256, 5514
BoxI	GACNNNNGTC	1 cut(s)	4047
BpiI	GAAGAC	5 cut(s)	1504, 2927, 3399, 6421, 6543
BplI	GAGNNNNNCTC	6 cut(s)	3110, 3142, 4296, 4328, 4874, 4906
BpmI	CTGGAG	5 cut(s)	671, 1458, 1704, 3541, 3644
Bpu10I	CCTNAGC	6 cut(s)	75, 3258, 3888, 4695, 5694, 6039
Bpu1102I	GCTNAGC	3 cut(s)	104, 1005, 2349
Bpu14I	TTCGAA	1 cut(s)	3925
BpuEI	CTTGAG	8 cut(s)	770, 1076, 1211, 1613, 2333, 4352, 5480, 6230
BpuMI	CCSGG	6 cut(s)	222, 302, 1404, 2745, 3273, 5081
Bsa29I	ATCGAT	1 cut(s)	6376
BsaAI	YACGTR	2 cut(s)	1079, 6221
BsaBI	GATNNNNATC	2 cut(s)	3040, 5901
BsaHI	GRCGYC	1 cut(s)	5109
BsaI	GGTCTC	2 cut(s)	2153, 3605
BsaWI	WCCGGW	5 cut(s)	45, 252, 350, 4580, 4927
Bse118I	RCCGGY	3 cut(s)	3566, 4580, 6447
Bse21I	CCTNAGG	1 cut(s)	4877
Bse3DI	GCAATG	8 cut(s)	934, 3030, 3069, 3315, 3997, 4189, 4357, 6321
Bse8I	GATNNNNATC	2 cut(s)	3040, 5901
BseAI	TCCGGA	3 cut(s)	45, 252, 4927
BseCI	ATCGAT	1 cut(s)	6376
BseJI	GATNNNNATC	2 cut(s)	3040, 5901
BseMI	GCAATG	8 cut(s)	934, 3030, 3069, 3315, 3997, 4189, 4357, 6321
BseRI	GAGGAG	8 cut(s)	429, 2171, 3184, 3905, 4898, 5318, 5783, 6296
BseSI	GKGCMC	4 cut(s)	2593, 4867, 6061, 6114
BseX3I	CGGCCG	1 cut(s)	223
BseYI	CCCAGC	7 cut(s)	526, 989, 993, 3581, 5371, 5540, 6437
BsgI	GTGCAG	7 cut(s)	182, 2415, 2772, 3738, 4044, 4308, 6486
Bsh1236I	CGCG	6 cut(s)	68, 129, 2190, 2323, 2356, 5203
Bsh1285I	CGRYCG	2 cut(s)	226, 1403
BshNI	GGYRCC	5 cut(s)	1747, 3742, 4463, 5479, 6058
BshTI	ACCGGT	1 cut(s)	4580
BshVI	ATCGAT	1 cut(s)	6376
BsiEI	CGRYCG	2 cut(s)	226, 1403
BsiHKAI	GWGCWC	5 cut(s)	1006, 1294, 1629, 2593, 4867
BsiHKCI	CYCGRG	6 cut(s)	287, 856, 2730, 5330, 5906, 6332
BsmBI	CGTCTC	1 cut(s)	2033
BsmI	GAATGC	2 cut(s)	2541, 3583
Bso31I	GGTCTC	2 cut(s)	2153, 3605
BsoBI	CYCGRG	6 cut(s)	287, 856, 2730, 5330, 5906, 6332
Bsp119I	TTCGAA	1 cut(s)	3925
Bsp1286I	GDGCHC	9 cut(s)	1006, 1294, 1629, 2593, 3442, 4867, 6061, 6114, 6278
Bsp13I	TCCGGA	3 cut(s)	45, 252, 4927
Bsp1407I	TGTACA	1 cut(s)	762
Bsp1720I	GCTNAGC	3 cut(s)	104, 1005, 2349
Bsp19I	CCATGG	5 cut(s)	1325, 1735, 3590, 4087, 6001
BspDI	ATCGAT	1 cut(s)	6376
BspEI	TCCGGA	3 cut(s)	45, 252, 4927
BspFNI	CGCG	6 cut(s)	68, 129, 2190, 2323, 2356, 5203
BspHI	TCATGA	4 cut(s)	1651, 3373, 3412, 4216
BspMAI	CTGCAG	2 cut(s)	1490, 4042
BspMI	ACCTGC	3 cut(s)	2995, 3175, 4042
BspOI	GCTAGC	1 cut(s)	5549
BspQI	GCTCTTC	1 cut(s)	4158
BspT104I	TTCGAA	1 cut(s)	3925
BspT107I	GGYRCC	5 cut(s)	1747, 3742, 4463, 5479, 6058
BspTI	CTTAAG	2 cut(s)	3419, 4979
BspTNI	GGTCTC	2 cut(s)	2153, 3605
BsrBI	CCGCTC	3 cut(s)	125, 228, 286
BsrDI	GCAATG	8 cut(s)	934, 3030, 3069, 3315, 3997, 4189, 4357, 6321
BsrFI	RCCGGY	3 cut(s)	3566, 4580, 6447
BsrGI	TGTACA	1 cut(s)	762
BssAI	RCCGGY	3 cut(s)	3566, 4580, 6447
BssNI	GRCGYC	1 cut(s)	5109
BssSI	CACGAG	2 cut(s)	319, 2691
Bst2BI	CACGAG	2 cut(s)	319, 2691
Bst6I	CTCTTC	6 cut(s)	3147, 3682, 3717, 4158, 4935, 5039
BstACI	GRCGYC	1 cut(s)	5109
BstAFI	CTTAAG	2 cut(s)	3419, 4979
BstAPI	GCANNNNNTGC	1 cut(s)	6122
BstAUI	TGTACA	1 cut(s)	762
BstBAI	YACGTR	2 cut(s)	1079, 6221
BstBI	TTCGAA	1 cut(s)	3925
BstDSI	CCRYGG	7 cut(s)	1325, 1735, 2929, 3590, 4087, 5483, 6001
BstEII	GGTNACC	1 cut(s)	4222
BstENI	CCTNNNNNAGG	5 cut(s)	2167, 4880, 5382, 5765, 6524
BstFNI	CGCG	6 cut(s)	68, 129, 2190, 2323, 2356, 5203
BstH2I	RGCGCY	1 cut(s)	757
BstHHI	GCGC	5 cut(s)	131, 756, 2319, 5203, 5801
BstMCI	CGRYCG	2 cut(s)	226, 1403
BstNSI	RCATGY	4 cut(s)	262, 2447, 3512, 3863
BstPAI	GACNNNNGTC	1 cut(s)	4047
BstPI	GGTNACC	1 cut(s)	4222
BstSFI	CTRYAG	5 cut(s)	1486, 2421, 4038, 4140, 5793
BstSLI	GKGCMC	4 cut(s)	2593, 4867, 6061, 6114
BstUI	CGCG	6 cut(s)	68, 129, 2190, 2323, 2356, 5203
BstV2I	GAAGAC	5 cut(s)	1504, 2927, 3399, 6421, 6543
BstX2I	RGATCY	9 cut(s)	185, 919, 1276, 2172, 2457, 3820, 4705, 5122, 6142
BstXI	CCANNNNNNTGG	2 cut(s)	3668, 4301
BstYI	RGATCY	9 cut(s)	185, 919, 1276, 2172, 2457, 3820, 4705, 5122, 6142
BstZI	CGGCCG	1 cut(s)	223
Bsu15I	ATCGAT	1 cut(s)	6376
Bsu36I	CCTNAGG	1 cut(s)	4877
BsuTUI	ATCGAT	1 cut(s)	6376
BtgI	CCRYGG	7 cut(s)	1325, 1735, 2929, 3590, 4087, 5483, 6001
BtgZI	GCGATG	1 cut(s)	320
BtrI	CACGTC	1 cut(s)	1962
BtsI	GCAGTG	1 cut(s)	3336
BveI	ACCTGC	3 cut(s)	2995, 3175, 4042
CaiI	CAGNNNCTG	3 cut(s)	698, 2759, 4058
CciI	TCATGA	4 cut(s)	1651, 3373, 3412, 4216
CfoI	GCGC	5 cut(s)	131, 756, 2319, 5203, 5801
Cfr10I	RCCGGY	3 cut(s)	3566, 4580, 6447
ClaI	ATCGAT	1 cut(s)	6376
CseI	GACGC	5 cut(s)	57, 1371, 3439, 4757, 5098
CsiI	ACCWGGT	1 cut(s)	5173
CspAI	ACCGGT	1 cut(s)	4580
CspCI	CAANNNNNGTGG	6 cut(s)	2119, 2154, 2911, 2946, 6399, 6434
DraI	TTTAAA	3 cut(s)	2947, 3933, 5004
DraIII	CACNNNGTG	2 cut(s)	5250, 5381
EagI	CGGCCG	1 cut(s)	223
Eam1104I	CTCTTC	6 cut(s)	3147, 3682, 3717, 4158, 4935, 5039
EarI	CTCTTC	6 cut(s)	3147, 3682, 3717, 4158, 4935, 5039
EciI	GGCGGA	2 cut(s)	256, 356
Ecl136II	GAGCTC	1 cut(s)	1627
EclXI	CGGCCG	1 cut(s)	223
Eco147I	AGGCCT	2 cut(s)	4889, 5050
Eco24I	GRGCYC	3 cut(s)	1629, 3442, 6278
Eco31I	GGTCTC	2 cut(s)	2153, 3605
Eco32I	GATATC	2 cut(s)	2565, 3379
Eco52I	CGGCCG	1 cut(s)	223
Eco53kI	GAGCTC	1 cut(s)	1627
Eco57I	CTGAAG	9 cut(s)	920, 1245, 1269, 1775, 2031, 2202, 2643, 2828, 3486
Eco72I	CACGTG	1 cut(s)	1079
Eco81I	CCTNAGG	1 cut(s)	4877
Eco88I	CYCGRG	6 cut(s)	287, 856, 2730, 5330, 5906, 6332
Eco91I	GGTNACC	1 cut(s)	4222
EcoICRI	GAGCTC	1 cut(s)	1627
EcoNI	CCTNNNNNAGG	5 cut(s)	2167, 4880, 5382, 5765, 6524
EcoO109I	RGGNCCY	4 cut(s)	1666, 2042, 3552, 5815
EcoO65I	GGTNACC	1 cut(s)	4222
EcoRI	GAATTC	2 cut(s)	676, 3331
EcoRV	GATATC	2 cut(s)	2565, 3379
EcoT22I	ATGCAT	1 cut(s)	4969
EcoT38I	GRGCYC	3 cut(s)	1629, 3442, 6278
Esp3I	CGTCTC	1 cut(s)	2033
FauI	CCCGC	2 cut(s)	234, 5670
FauNDI	CATATG	1 cut(s)	842
FbaI	TGATCA	3 cut(s)	4558, 4906, 5557
FblI	GTMKAC	2 cut(s)	1464, 3969
FriOI	GRGCYC	3 cut(s)	1629, 3442, 6278
FspBI	CTAG	9 cut(s)	1199, 2300, 2655, 3489, 4470, 4868, 5052, 5546, 6311
GlaI	GCGC	5 cut(s)	130, 755, 2318, 5202, 5800
GsaI	CCCAGC	7 cut(s)	530, 993, 997, 3585, 5375, 5544, 6441
GsuI	CTGGAG	5 cut(s)	671, 1458, 1704, 3541, 3644
HaeII	RGCGCY	1 cut(s)	757
HgaI	GACGC	5 cut(s)	57, 1371, 3439, 4757, 5098
HhaI	GCGC	5 cut(s)	131, 756, 2319, 5203, 5801
Hin1I	GRCGYC	1 cut(s)	5109
Hin6I	GCGC	5 cut(s)	129, 754, 2317, 5201, 5799
HinP1I	GCGC	5 cut(s)	129, 754, 2317, 5201, 5799
HincII	GTYRAC	5 cut(s)	1063, 3970, 5683, 5707, 6511
HindII	GTYRAC	5 cut(s)	1063, 3970, 5683, 5707, 6511
HindIII	AAGCTT	5 cut(s)	1880, 2604, 2840, 4780, 5887
HpaI	GTTAAC	2 cut(s)	1063, 5683
Hpy99I	CGWCG	2 cut(s)	218, 6343
HpyCH4IV	ACGT	9 cut(s)	1078, 1467, 1961, 2137, 2251, 2378, 4231, 4653, 6220
HpySE526I	ACGT	9 cut(s)	1078, 1467, 1961, 2137, 2251, 2378, 4231, 4653, 6220
Hsp92I	GRCGYC	1 cut(s)	5109
HspAI	GCGC	5 cut(s)	129, 754, 2317, 5201, 5799
KflI	GGGWCCC	1 cut(s)	1666
Kpn2I	TCCGGA	3 cut(s)	45, 252, 4927
KroI	GCCGGC	1 cut(s)	6447
KroNI	GCCGGC	1 cut(s)	6449
Ksp22I	TGATCA	3 cut(s)	4558, 4906, 5557
KspAI	GTTAAC	2 cut(s)	1063, 5683
LguI	GCTCTTC	1 cut(s)	4158
MabI	ACCWGGT	1 cut(s)	5173
MaeI	CTAG	9 cut(s)	1199, 2300, 2655, 3489, 4470, 4868, 5052, 5546, 6311
MaeII	ACGT	9 cut(s)	1078, 1467, 1961, 2137, 2251, 2378, 4231, 4653, 6220
MbiI	CCGCTC	3 cut(s)	125, 228, 286
MfeI	CAATTG	4 cut(s)	1143, 1932, 3387, 6288
MflI	RGATCY	9 cut(s)	185, 919, 1276, 2172, 2457, 3820, 4705, 5122, 6142
MhlI	GDGCHC	9 cut(s)	1006, 1294, 1629, 2593, 3442, 4867, 6061, 6114, 6278
MlsI	TGGCCA	8 cut(s)	624, 1026, 1445, 3068, 3672, 3698, 4790, 6076
MluNI	TGGCCA	8 cut(s)	624, 1026, 1445, 3068, 3672, 3698, 4790, 6076
MlyI	GAGTC	9 cut(s)	311, 580, 623, 1389, 1695, 1740, 4732, 5319, 6568
Mox20I	TGGCCA	8 cut(s)	624, 1026, 1445, 3068, 3672, 3698, 4790, 6076
Mph1103I	ATGCAT	1 cut(s)	4969
MroI	TCCGGA	3 cut(s)	45, 252, 4927
MroNI	GCCGGC	1 cut(s)	6447
MroXI	GAANNNNTTC	4 cut(s)	155, 158, 778, 4248
MscI	TGGCCA	8 cut(s)	624, 1026, 1445, 3068, 3672, 3698, 4790, 6076
Msp20I	TGGCCA	8 cut(s)	624, 1026, 1445, 3068, 3672, 3698, 4790, 6076
MspA1I	CMGCKG	5 cut(s)	526, 883, 993, 4256, 6500
MspCI	CTTAAG	2 cut(s)	3419, 4979
MunI	CAATTG	4 cut(s)	1143, 1932, 3387, 6288
Mva1269I	GAATGC	2 cut(s)	2541, 3583
MvnI	CGCG	6 cut(s)	68, 129, 2190, 2323, 2356, 5203
NaeI	GCCGGC	1 cut(s)	6449
NciI	CCSGG	6 cut(s)	222, 302, 1404, 2745, 3273, 5081
NcoI	CCATGG	5 cut(s)	1325, 1735, 3590, 4087, 6001
NdeI	CATATG	1 cut(s)	842
NgoMIV	GCCGGC	1 cut(s)	6447
NheI	GCTAGC	1 cut(s)	5545
NmeAIII	GCCGAG	1 cut(s)	637
NsiI	ATGCAT	1 cut(s)	4969
NspI	RCATGY	4 cut(s)	262, 2447, 3512, 3863
NspV	TTCGAA	1 cut(s)	3925
OliI	CACNNNNGTG	1 cut(s)	2219
PaeI	GCATGC	1 cut(s)	3512
PaeR7I	CTCGAG	3 cut(s)	856, 5330, 5906
PagI	TCATGA	4 cut(s)	1651, 3373, 3412, 4216
PasI	CCCWGGG	1 cut(s)	3434
PceI	AGGCCT	2 cut(s)	4889, 5050
PciI	ACATGT	1 cut(s)	3859
PciSI	GCTCTTC	1 cut(s)	4158
PcsI	WCGNNNNNNNCGW	3 cut(s)	95, 294, 6094
PctI	GAATGC	2 cut(s)	2541, 3583
PdiI	GCCGGC	1 cut(s)	6449
PdmI	GAANNNNTTC	4 cut(s)	155, 158, 778, 4248
PflFI	GACNNNGTC	2 cut(s)	1404, 1664
PflMI	CCANNNNNTGG	2 cut(s)	1331, 4093
PinAI	ACCGGT	1 cut(s)	4580
PleI	GAGTC	9 cut(s)	311, 579, 622, 1389, 1694, 1739, 4732, 5319, 6567
PmaCI	CACGTG	1 cut(s)	1079
PmlI	CACGTG	1 cut(s)	1079
PpsI	GAGTC	9 cut(s)	311, 579, 622, 1389, 1694, 1739, 4732, 5319, 6567
Ppu21I	YACGTR	2 cut(s)	1079, 6221
PpuMI	RGGWCCY	3 cut(s)	1666, 2042, 5815
PscI	ACATGT	1 cut(s)	3859
PshAI	GACNNNNGTC	1 cut(s)	4047
PshBI	ATTAAT	1 cut(s)	3407
Psp124BI	GAGCTC	1 cut(s)	1629
Psp1406I	AACGTT	1 cut(s)	4231
Psp5II	RGGWCCY	3 cut(s)	1666, 2042, 5815
PspCI	CACGTG	1 cut(s)	1079
PspEI	GGTNACC	1 cut(s)	4222
PspFI	CCCAGC	7 cut(s)	526, 989, 993, 3581, 5371, 5540, 6437
PspPPI	RGGWCCY	3 cut(s)	1666, 2042, 5815
PspXI	VCTCGAGB	1 cut(s)	856
PstI	CTGCAG	2 cut(s)	1490, 4042
PstNI	CAGNNNCTG	3 cut(s)	698, 2759, 4058
PsuI	RGATCY	9 cut(s)	185, 919, 1276, 2172, 2457, 3820, 4705, 5122, 6142
PsyI	GACNNNGTC	2 cut(s)	1404, 1664
PvuII	CAGCTG	4 cut(s)	883, 993, 4256, 6500
SacI	GAGCTC	1 cut(s)	1629
SalI	GTCGAC	1 cut(s)	3968
SapI	GCTCTTC	1 cut(s)	4158
ScaI	AGTACT	3 cut(s)	2513, 3828, 4800
SchI	GAGTC	9 cut(s)	311, 580, 623, 1389, 1695, 1740, 4732, 5319, 6568
SduI	GDGCHC	9 cut(s)	1006, 1294, 1629, 2593, 3442, 4867, 6061, 6114, 6278
SexAI	ACCWGGT	1 cut(s)	5173
SfcI	CTRYAG	5 cut(s)	1486, 2421, 4038, 4140, 5793
SfiI	GGCCNNNNNGGCC	1 cut(s)	6082
Sfr274I	CTCGAG	3 cut(s)	856, 5330, 5906
SfuI	TTCGAA	1 cut(s)	3925
SlaI	CTCGAG	3 cut(s)	856, 5330, 5906
SpeI	ACTAGT	1 cut(s)	4469
SphI	GCATGC	1 cut(s)	3512
SseBI	AGGCCT	2 cut(s)	4889, 5050
SspI	AATATT	3 cut(s)	1304, 1577, 4067
SspMI	CTAG	9 cut(s)	1199, 2300, 2655, 3489, 4470, 4868, 5052, 5546, 6311
SstI	GAGCTC	1 cut(s)	1629
StuI	AGGCCT	2 cut(s)	4889, 5050
TaiI	ACGT	9 cut(s)	1081, 1470, 1964, 2140, 2254, 2381, 4234, 4656, 6223
TaqII	GACCGA	1 cut(s)	1970
TauI	GCSGC	9 cut(s)	11, 182, 228, 286, 2356, 2876, 3651, 3707, 6586
TspGWI	ACGGA	6 cut(s)	22, 49, 392, 785, 2918, 4296
Tth111I	GACNNNGTC	2 cut(s)	1404, 1664
Van91I	CCANNNNNTGG	2 cut(s)	1331, 4093
Vha464I	CTTAAG	2 cut(s)	3419, 4979
VneI	GTGCAC	2 cut(s)	2589, 4863
VspI	ATTAAT	1 cut(s)	3407
XagI	CCTNNNNNAGG	5 cut(s)	2167, 4880, 5382, 5765, 6524
XapI	RAATTY	8 cut(s)	676, 2942, 2964, 3331, 4274, 4673, 4829, 6022
XceI	RCATGY	4 cut(s)	262, 2447, 3512, 3863
XcmI	CCANNNNNNNNNTGG	5 cut(s)	632, 3343, 3680, 4798, 6069
XhoI	CTCGAG	3 cut(s)	856, 5330, 5906
XmiI	GTMKAC	2 cut(s)	1464, 3969
XmnI	GAANNNNTTC	4 cut(s)	155, 158, 778, 4248
XspI	CTAG	9 cut(s)	1199, 2300, 2655, 3489, 4470, 4868, 5052, 5546, 6311
ZrmI	AGTACT	3 cut(s)	2513, 3828, 4800
Zsp2I	ATGCAT	1 cut(s)	4969

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.