AT5G53970

aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005586
Physical Location & Seq
Forward (+)
21910471 .. 21912809
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53970.1

Sequence Viewer

Length: 1245 bp
ATGGAGAATGGAGCAACGACGACGAGCACAATTACCATCAAAGGGATTCTGAGTTTGCTAATGGAAAGCATCACAACAGAGGAAGATGAAGGAGGAAAGAGAGTAATATCTCTGGGAATGGGAGACCCAACACTCTACTCGTGTTTTCGTACAACACAAGTCTCTCTTCAAGCTGTTTCTGATTCTCTTCTCTCCAACAAGTTCCATGGTTACTCTCCTACCGTCGGTCTTCCCCAAGCTCGAAGGGCAATAGCAGAGTATCTATCGCGTGATCTTCCATACAAACTTTCACAGGATGATGTGTTTATCACATCGGGTTGCACGCAAGCGATCGATGTAGCATTGTCGATGTTAGCTCGTCCCAGGGCTAATATACTTCTTCCAAGGCCTGGTTTCCCAATCTATGAACTCTGTGCTAAGTTTAGACACCTTGAAGTTCGCTACGTCGATCTTCTTCCGGAAAATGGATGGGAGATCGATCTTGATGCTGTCGAGGCTCTTGCAGACGAAAACACGGTTGCTTTGGTTGTTATAAACCCTGGTAATCCTTGCGGGAATGTCTATAGCTACCAGCATTTGATGAAGATTGCGGAATCGGCGAAAAAACTAGGGTTTCTTGTGATTGCTGATGAGGTTTACGGTCATCTTGCTTTTGGTAGCAAACCGTTTGTGCCAATGGGTGTGTTTGGATCTATTGTTCCTGTGCTTACTCTTGGCTCTTTATCAAAGAGATGGATAGTTCCAGGTTGGCGACTCGGGTGGTTTGTCACCACTGATCCTTCTGGTTCCTTTAAGGACCCTAAGATCATTGAGAGGTTTAAGAAATACTTTGATATTCTTGGTGGACCAGCTACATTTATTCAGGCTGCAGTTCCCACTATTTTGGAACAGACGGATGAGTCTTTCTTCAAGAAAACCTTGAACTCGTTGAAGAACTCTTCGGATATTTGTTGTGACTGGATCAAGGAGATTCCTTGCATTGATTCCTCGCATCGACCAGAAGGATCCATGGCAATGATGGTTAAGCTGAATCTCTCATTACTTGAAGATGTAAGTGACGATATCGACTTCTGTTTCAAGTTAGCTAGGGAAGAATCAGTCATCCTTCTTCCTGGGACCGCGGTGGGGCTGAAGAACTGGCTGAGGATAACGTTTGCAGCAGATGCAACTTCGATTGAAGAAGCTTTTAAAAGGATCAAATGTTTCTATCTTAGACATGCCAAGACTCAATATCCAACCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001558 GO:0001560 GO:0003674 GO:0003824 GO:0004121 GO:0004838 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006082 GO:0006520 GO:0006570 GO:0006572 GO:0006725 GO:0006766 GO:0006775 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0007154 GO:0007275 GO:0008150 GO:0008152 GO:0008483 GO:0009056 GO:0009058 GO:0009063 GO:0009072 GO:0009074 GO:0009110 GO:0009605 GO:0009611 GO:0009628 GO:0009636 GO:0009651 GO:0009683 GO:0009684 GO:0009719 GO:0009725 GO:0009737 GO:0009753 GO:0009850 GO:0009851 GO:0009987 GO:0009991 GO:0010033 GO:0010188 GO:0010189 GO:0010817 GO:0016020 GO:0016053 GO:0016054 GO:0016143 GO:0016144 GO:0016740 GO:0016769 GO:0016829 GO:0016846 GO:0018130 GO:0019438 GO:0019439 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022622 GO:0031668 GO:0032501 GO:0032502 GO:0032787 GO:0033993 GO:0034641 GO:0034754 GO:0040008 GO:0042221 GO:0042360 GO:0042362 GO:0042430 GO:0042435 GO:0042445 GO:0042446 GO:0042538 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046394 GO:0046395 GO:0046483 GO:0048046 GO:0048364 GO:0048731 GO:0048830 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051716 GO:0065007 GO:0065008 GO:0070547 GO:0071496 GO:0071704 GO:0072330 GO:0080108 GO:0097305 GO:0099402 GO:1901135 GO:1901137 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901566 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1901615 GO:1901617 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00130 ko00270 ko00350 ko00360 ko00380 ko00400 ko00401 ko00950 ko00960 ko00966 ko01100 ko01110 ko01130 ko01210 ko01230 map00130 map00270 map00350 map00360 map00380 map00400 map00401 map00950 map00960 map00966 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

414

Amino Acids

45.9

Weight (kDa)

5.66

Isoelectric Point (pI)

37.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 34 - 399 1.1e-57 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
AasI GACNNNNNNGTC 1 cut(s) 898
AccB7I CCANNNNNTGG 1 cut(s) 389
AccII CGCG 2 cut(s) 268, 1121
AccIII TCCGGA 1 cut(s) 457
AciI CCGC 4 cut(s) 552, 590, 1119, 1121
AclI AACGTT 1 cut(s) 1151
AclWI GGATC 6 cut(s) 697, 770, 968, 999, 1012, 1202
AcuI CTGAAG 1 cut(s) 1151
AfaI GTAC 1 cut(s) 151
AfiI CCNNNNNNNGG 5 cut(s) 42, 224, 389, 464, 1125
AgsI TTSAA 8 cut(s) 170, 434, 910, 922, 931, 1046, 1078, 1178
AjnI CCWGG 5 cut(s) 362, 388, 538, 742, 1111
AloI GAACNNNNNNTCC 2 cut(s) 681, 713
AluBI AGCT 8 cut(s) 173, 239, 356, 567, 851, 1027, 1085, 1184
AluI AGCT 8 cut(s) 173, 239, 356, 567, 851, 1027, 1085, 1184
Alw21I GWGCWC 1 cut(s) 29
Alw26I GTCTC 2 cut(s) 117, 166
AlwI GGATC 6 cut(s) 697, 770, 968, 999, 1012, 1202
Ama87I CYCGRG 1 cut(s) 755
Aor13HI TCCGGA 1 cut(s) 457
AoxI GGCC 1 cut(s) 386
ApeKI GCWGC 2 cut(s) 866, 1157
AspS9I GGNCC 3 cut(s) 796, 845, 1116
AsuHPI GGTGA 1 cut(s) 760
AvaI CYCGRG 1 cut(s) 755
AvaII GGWCC 3 cut(s) 796, 845, 1116
BamHI GGATCC 1 cut(s) 1004
BauI CACGAG 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 221
Bbv12I GWGCWC 1 cut(s) 29
BbvCI CCTCAGC 1 cut(s) 1142
BbvI GCAGC 2 cut(s) 853, 1169
BccI CCATC 4 cut(s) 44, 462, 726, 1012
BcgI CGANNNNNNTGC 4 cut(s) 467, 482, 501, 516
BciT130I CCWGG 5 cut(s) 364, 390, 540, 744, 1113
BcoDI GTCTC 2 cut(s) 117, 166
BfaI CTAG 2 cut(s) 608, 1086
BfmI CTRYAG 2 cut(s) 562, 867
BisI GCNGC 2 cut(s) 867, 1158
BlsI GCNGC 2 cut(s) 868, 1159
Bme1390I CCNGG 5 cut(s) 364, 390, 540, 744, 1113
Bme18I GGWCC 3 cut(s) 796, 845, 1116
BmeT110I CYCGRG 1 cut(s) 755
BmgT120I GGNCC 3 cut(s) 796, 845, 1116
BmiI GGNNCC 4 cut(s) 787, 798, 1006, 1117
BmrFI CCNGG 5 cut(s) 364, 390, 540, 744, 1113
BmsI GCATC 4 cut(s) 78, 475, 1000, 1153
BpiI GAAGAC 1 cut(s) 221
Bpu10I CCTNAGC 1 cut(s) 1142
Bsa29I ATCGAT 2 cut(s) 333, 477
BsaI GGTCTC 1 cut(s) 117
BsaJI CCNNGG 8 cut(s) 205, 362, 363, 383, 538, 1008, 1112, 1119
BsaWI WCCGGW 1 cut(s) 457
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 224, 389, 464, 1125
Bse1I ACTGG 2 cut(s) 962, 1142
Bse3DI GCAATG 1 cut(s) 1020
BseAI TCCGGA 1 cut(s) 457
BseBI CCWGG 5 cut(s) 364, 390, 540, 744, 1113
BseCI ATCGAT 2 cut(s) 333, 477
BseDI CCNNGG 8 cut(s) 205, 362, 363, 383, 538, 1008, 1112, 1119
BseGI GGATG 4 cut(s) 301, 473, 901, 1101
BseLI CCNNNNNNNGG 5 cut(s) 42, 224, 389, 464, 1125
BseMI GCAATG 1 cut(s) 1020
BseMII CTCAG 2 cut(s) 41, 1133
BseNI ACTGG 2 cut(s) 962, 1142
BseXI GCAGC 2 cut(s) 853, 1169
Bsh1236I CGCG 2 cut(s) 268, 1121
Bsh1285I CGRYCG 1 cut(s) 333
BshFI GGCC 1 cut(s) 388
BshVI ATCGAT 2 cut(s) 333, 477
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 29
BsiHKCI CYCGRG 1 cut(s) 755
BsiSI CCGG 1 cut(s) 458
BslFI GGGAC 2 cut(s) 345, 1129
BslI CCNNNNNNNGG 5 cut(s) 42, 224, 389, 464, 1125
BsmAI GTCTC 2 cut(s) 117, 166
BsmFI GGGAC 2 cut(s) 345, 1129
BsnI GGCC 1 cut(s) 388
Bso31I GGTCTC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 755
Bsp1286I GDGCHC 1 cut(s) 29
Bsp13I TCCGGA 1 cut(s) 457
Bsp19I CCATGG 2 cut(s) 205, 1008
BspACI CCGC 4 cut(s) 552, 590, 1119, 1121
BspANI GGCC 1 cut(s) 388
BspCNI CTCAG 2 cut(s) 42, 1134
BspDI ATCGAT 2 cut(s) 333, 477
BspEI TCCGGA 1 cut(s) 457
BspFNI CGCG 2 cut(s) 268, 1121
BspLI GGNNCC 4 cut(s) 787, 798, 1006, 1117
BspMAI CTGCAG 1 cut(s) 871
BspPI GGATC 6 cut(s) 697, 770, 968, 999, 1012, 1202
BspTNI GGTCTC 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 1020
BsrI ACTGG 2 cut(s) 962, 1142
BssECI CCNNGG 8 cut(s) 205, 362, 363, 383, 538, 1008, 1112, 1119
BssSI CACGAG 1 cut(s) 139
BssT1I CCWWGG 3 cut(s) 205, 383, 1008
Bst2BI CACGAG 1 cut(s) 139
Bst2UI CCWGG 5 cut(s) 364, 390, 540, 744, 1113
Bst4CI ACNGT 4 cut(s) 223, 517, 641, 666
Bst6I CTCTTC 3 cut(s) 171, 192, 943
BstAPI GCANNNNNTGC 1 cut(s) 1163
BstC8I GCNNGC 2 cut(s) 323, 327
BstDEI CTNAG 5 cut(s) 50, 417, 801, 1142, 1211
BstDSI CCRYGG 3 cut(s) 205, 1008, 1119
BstF5I GGATG 4 cut(s) 301, 473, 901, 1101
BstFNI CGCG 2 cut(s) 268, 1121
BstMAI GTCTC 2 cut(s) 117, 166
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 4 cut(s) 245, 494, 596, 1163
BstNI CCWGG 5 cut(s) 364, 390, 540, 744, 1113
BstNSI RCATGY 1 cut(s) 1220
BstSCI CCNGG 5 cut(s) 362, 388, 538, 742, 1111
BstSFI CTRYAG 2 cut(s) 562, 867
BstUI CGCG 2 cut(s) 268, 1121
BstV1I GCAGC 2 cut(s) 853, 1169
BstV2I GAAGAC 1 cut(s) 221
BstX2I RGATCY 2 cut(s) 689, 1004
BstXI CCANNNNNNTGG 1 cut(s) 883
BstYI RGATCY 2 cut(s) 689, 1004
Bsu15I ATCGAT 2 cut(s) 333, 477
BsuRI GGCC 1 cut(s) 388
BsuTUI ATCGAT 2 cut(s) 333, 477
BtgI CCRYGG 3 cut(s) 205, 1008, 1119
BtsCI GGATG 4 cut(s) 301, 473, 901, 1101
BtsIMutI CAGTG 1 cut(s) 771
Cac8I GCNNGC 2 cut(s) 323, 327
Cfr13I GGNCC 3 cut(s) 796, 845, 1116
Cfr42I CCGCGG 1 cut(s) 1122
ClaI ATCGAT 2 cut(s) 333, 477
Csp6I GTAC 1 cut(s) 150
CviAII CATG 3 cut(s) 206, 1009, 1217
CviQI GTAC 1 cut(s) 150
DdeI CTNAG 5 cut(s) 50, 417, 801, 1142, 1211
DraI TTTAAA 1 cut(s) 1189
DrdI GACNNNNNNGTC 1 cut(s) 898
DseDI GACNNNNNNGTC 1 cut(s) 898
Eam1104I CTCTTC 3 cut(s) 171, 192, 943
EarI CTCTTC 3 cut(s) 171, 192, 943
Eco130I CCWWGG 3 cut(s) 205, 383, 1008
Eco147I AGGCCT 1 cut(s) 388
Eco31I GGTCTC 1 cut(s) 117
Eco32I GATATC 1 cut(s) 1063
Eco47I GGWCC 3 cut(s) 796, 845, 1116
Eco57I CTGAAG 1 cut(s) 1151
Eco88I CYCGRG 1 cut(s) 755
EcoO109I RGGNCCY 1 cut(s) 796
EcoRII CCWGG 5 cut(s) 362, 388, 538, 742, 1111
EcoRV GATATC 1 cut(s) 1063
EcoT14I CCWWGG 3 cut(s) 205, 383, 1008
ErhI CCWWGG 3 cut(s) 205, 383, 1008
FaeI CATG 3 cut(s) 209, 1012, 1220
FalI AAGNNNNNCTT 6 cut(s) 150, 182, 812, 844, 902, 934
FaqI GGGAC 2 cut(s) 345, 1129
FatI CATG 3 cut(s) 205, 1008, 1216
FauI CCCGC 1 cut(s) 545
Fnu4HI GCNGC 2 cut(s) 867, 1158
FokI GGATG 4 cut(s) 308, 480, 908, 1088
Fsp4HI GCNGC 2 cut(s) 867, 1158
FspBI CTAG 2 cut(s) 608, 1086
GluI GCNGC 2 cut(s) 867, 1158
HaeIII GGCC 1 cut(s) 388
HapII CCGG 1 cut(s) 458
Hin1II CATG 3 cut(s) 209, 1012, 1220
HindIII AAGCTT 1 cut(s) 1182
HpaII CCGG 1 cut(s) 458
HphI GGTGA 1 cut(s) 760
Hpy166II GTNNAC 2 cut(s) 637, 845
Hpy188I TCNGA 3 cut(s) 51, 181, 943
Hpy188III TCNNGA 3 cut(s) 458, 482, 910
Hpy8I GTNNAC 2 cut(s) 637, 845
Hpy99I CGWCG 4 cut(s) 22, 25, 227, 449
HpyAV CCTTC 5 cut(s) 83, 237, 789, 995, 1115
HpyCH4III ACNGT 4 cut(s) 223, 517, 641, 666
HpyCH4IV ACGT 2 cut(s) 444, 1151
HpyCH4V TGCA 6 cut(s) 321, 503, 869, 978, 1157, 1166
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 494, 596, 1163
HpyF3I CTNAG 5 cut(s) 50, 417, 801, 1142, 1211
HpySE526I ACGT 2 cut(s) 444, 1151
Hsp92II CATG 3 cut(s) 209, 1012, 1220
Kpn2I TCCGGA 1 cut(s) 457
KspI CCGCGG 1 cut(s) 1122
LmnI GCTCC 1 cut(s) 11
Lsp1109I GCAGC 2 cut(s) 853, 1169
LweI GCATC 4 cut(s) 78, 475, 1000, 1153
MaeI CTAG 2 cut(s) 608, 1086
MaeII ACGT 2 cut(s) 444, 1151
MaeIII GTNAC 4 cut(s) 209, 766, 953, 1055
MflI RGATCY 2 cut(s) 689, 1004
MhlI GDGCHC 1 cut(s) 29
MluCI AATT 1 cut(s) 30
MlyI GAGTC 3 cut(s) 747, 908, 1219
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 7 cut(s) 73, 86, 487, 625, 807, 997, 1137
MroI TCCGGA 1 cut(s) 457
MseI TTAA 4 cut(s) 792, 819, 1023, 1188
MslI CAYNNNNRTG 1 cut(s) 1013
MspA1I CMGCKG 1 cut(s) 1121
MspI CCGG 1 cut(s) 458
MspR9I CCNGG 5 cut(s) 364, 390, 540, 744, 1113
MvaI CCWGG 5 cut(s) 364, 390, 540, 744, 1113
MvnI CGCG 2 cut(s) 268, 1121
MwoI GCNNNNNNNGC 4 cut(s) 245, 494, 596, 1163
NcoI CCATGG 2 cut(s) 205, 1008
NlaIII CATG 3 cut(s) 209, 1012, 1220
NlaIV GGNNCC 4 cut(s) 787, 798, 1006, 1117
NmuCI GTSAC 3 cut(s) 766, 953, 1055
NspI RCATGY 1 cut(s) 1220
PasI CCCWGGG 1 cut(s) 363
PceI AGGCCT 1 cut(s) 388
PfeI GAWTC 7 cut(s) 46, 182, 593, 970, 983, 1030, 1094
PflMI CCANNNNNTGG 1 cut(s) 389
PkrI GCNGC 2 cut(s) 868, 1159
Ple19I CGATCG 1 cut(s) 333
PleI GAGTC 3 cut(s) 747, 907, 1219
PpsI GAGTC 3 cut(s) 747, 907, 1219
PpuMI RGGWCCY 1 cut(s) 796
PsiI TTATAA 1 cut(s) 533
Psp1406I AACGTT 1 cut(s) 1151
Psp5II RGGWCCY 1 cut(s) 796
Psp6I CCWGG 5 cut(s) 362, 388, 538, 742, 1111
PspGI CCWGG 5 cut(s) 362, 388, 538, 742, 1111
PspN4I GGNNCC 4 cut(s) 787, 798, 1006, 1117
PspPI GGNCC 3 cut(s) 796, 845, 1116
PspPPI RGGWCCY 1 cut(s) 796
PstI CTGCAG 1 cut(s) 871
PsuI RGATCY 2 cut(s) 689, 1004
PvuI CGATCG 1 cut(s) 333
RsaI GTAC 1 cut(s) 151
RsaNI GTAC 1 cut(s) 150
RseI CAYNNNNRTG 1 cut(s) 1013
SacII CCGCGG 1 cut(s) 1122
SaqAI TTAA 4 cut(s) 792, 819, 1023, 1188
SatI GCNGC 2 cut(s) 867, 1158
Sau96I GGNCC 3 cut(s) 796, 845, 1116
SchI GAGTC 3 cut(s) 747, 908, 1219
ScrFI CCNGG 5 cut(s) 364, 390, 540, 744, 1113
SduI GDGCHC 1 cut(s) 29
SfaNI GCATC 4 cut(s) 78, 475, 1000, 1153
SfcI CTRYAG 2 cut(s) 562, 867
Sfr303I CCGCGG 1 cut(s) 1122
SgrBI CCGCGG 1 cut(s) 1122
SinI GGWCC 3 cut(s) 796, 845, 1116
SmiMI CAYNNNNRTG 1 cut(s) 1013
Sse9I AATT 1 cut(s) 30
SseBI AGGCCT 1 cut(s) 388
SsiI CCGC 4 cut(s) 552, 590, 1119, 1121
SspMI CTAG 2 cut(s) 608, 1086
StuI AGGCCT 1 cut(s) 388
StyD4I CCNGG 5 cut(s) 362, 388, 538, 742, 1111
StyI CCWWGG 3 cut(s) 205, 383, 1008
TaaI ACNGT 4 cut(s) 223, 517, 641, 666
TaiI ACGT 2 cut(s) 447, 1154
TaqI TCGA 9 cut(s) 241, 333, 347, 447, 477, 492, 994, 1065, 1172
TaqII GACCGA 1 cut(s) 215
TasI AATT 1 cut(s) 30
TfiI GAWTC 7 cut(s) 46, 182, 593, 970, 983, 1030, 1094
Tru1I TTAA 4 cut(s) 792, 819, 1023, 1188
Tru9I TTAA 4 cut(s) 792, 819, 1023, 1188
TscAI CASTG 1 cut(s) 778
TseFI GTSAC 3 cut(s) 766, 953, 1055
TseI GCWGC 2 cut(s) 866, 1157
Tsp45I GTSAC 3 cut(s) 766, 953, 1055
TspDTI ATGAA 3 cut(s) 102, 420, 596
TspGWI ACGGA 1 cut(s) 908
TspRI CASTG 1 cut(s) 778
Van91I CCANNNNNTGG 1 cut(s) 389
VpaK11BI GGWCC 3 cut(s) 796, 845, 1116
XceI RCATGY 1 cut(s) 1220
XcmI CCANNNNNNNNNTGG 1 cut(s) 1015
XspI CTAG 2 cut(s) 608, 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.