AT5G54130

calcium-binding protein At1g02270-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006698
Physical Location & Seq
Forward (+)
21962570 .. 21965623
3054 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54130.2

Sequence Viewer

Length: 1311 bp
ATGAAGGGGAGGATATCAAAAATAGGAAGCTATGCAATCTCGTCTTCCATTAGAGATCAGCATCAGCAACCTTGTATTTCTTGTACTACTTTCAATATCCTTGCTCCGATCTATAAACGTCTCTCTCACAAGGATCAAAGTCTTCGTGAAAGTGATAATCGAGCGTATTGGCTTGGAAGGAATCATCGAATCATTGATTGGTTATTGTATGAGAGATCTTCAATCATATGTTTACAGGAGTTTTGGGTAGGCAATGAAGAGCTTGTTAATTTGTACGAGAAGAGACTTGGAGACGCTGGTTATCTAAGTTACAAGCTTGGTCGTACTAATAATCGTGGTGATGGTTTGCTTACGGCTGTTCATAAGGACTATTTTAGAGTTGTGAACTCGAGGGATTTGCTTTTTAATGATTGTGGTGATCGAGTTGCTCAGTTGCTACATGTAGAATTAGTTCCTCCTTACTCTCAGTATGATGCTCATCAAGAAGTTTTGATTGTGAATACTCATTTGTTGTTTCCTCATGATTCAACATTGTCCATAGTAAGATTGCAGCAGGTATATAAGATTCTTCAGTATGTAGAATCTTACCAGAAGGAAGTTAATCTCAGTCCCATGCCTATTATTCTTTGCGGAGATTGGAACGGAAGTAAGCGAGGACATGTCTACAAGTTTCTCAGGTCACAGGGTTTTGTGTCGTCTTACGATACTGCTCATCGGTACACAGACTCAGATGCTCACAAGTGGGTAAGCCACCGGAATCATCGGGGTAACATATGCGCTGTCGATTTCATATGGCTTTTAAATCCAAATCGGTATAGAAAGCTGCTAAAAACAAGTTGGAGCGAGGCAGTCTTTGGCATGTTCAGGTATCTACTGAGAAGAGCTTCACTAACAGCTGAAGATGCTTTTGCTTTTCTAAAAACTGACAATGATGGTGACCACATTACATTCATGGGCTTCTGTGAGACTCTTCGGCAGCTCAATCTAACAGGTCACTGTAATGGACTCACGACAAAAGAGATCAAAGATTTGTGGACTCAAGCTGACATTGATGGAAACGGTCTTCTAGATTATAAGGAGTTTCAGCAAAGAATATGGAACCAAACCTGGTCAGAACAAAGAGATGCAGAGGACGGAGAAGCAAAAGGTAACCAAGAACAAACCGTTGGATTCAGTGTCAAAAACGCAGTTTTGTTTCCTCCTGAAGTTGAAAAAGGAATGTGGCCAGAAAATTACTCTCTATCAGATCACGCGAGACTCACTGTTGTATTCTCACCCATAAGAATGCCTTGCTCTCAATTGGTTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

436

Amino Acids

50.53

Weight (kDa)

7.64

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 30 - 240 2.8e-10 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1074
Acc36I ACCTGC 1 cut(s) 544
AccI GTMKAC 1 cut(s) 663
AccII CGCG 1 cut(s) 1253
AciI CCGC 1 cut(s) 630
AclWI GGATC 1 cut(s) 141
AcoI YGGCCR 1 cut(s) 1223
AcuI CTGAAG 3 cut(s) 554, 918, 1224
AfaI GTAC 4 cut(s) 85, 275, 325, 719
AflIII ACRYGT 2 cut(s) 439, 658
AgsI TTSAA 4 cut(s) 94, 222, 528, 1211
AjnI CCWGG 1 cut(s) 1106
AjuI GAANNNNNNNTTGG 4 cut(s) 181, 213, 1149, 1181
AluBI AGCT 8 cut(s) 30, 262, 316, 823, 884, 896, 979, 1043
AluI AGCT 8 cut(s) 30, 262, 316, 823, 884, 896, 979, 1043
Alw26I GTCTC 5 cut(s) 125, 277, 285, 959, 1249
AlwI GGATC 1 cut(s) 141
Ama87I CYCGRG 1 cut(s) 388
AoxI GGCC 1 cut(s) 1223
ApeKI GCWGC 3 cut(s) 550, 823, 976
Asp700I GAANNNNTTC 2 cut(s) 450, 883
AspLEI GCGC 1 cut(s) 779
AsuHPI GGTGA 4 cut(s) 350, 428, 947, 1266
AvaI CYCGRG 1 cut(s) 388
BalI TGGCCA 1 cut(s) 1225
BbsI GAAGAC 3 cut(s) 36, 134, 1055
BbvI GCAGC 3 cut(s) 562, 810, 988
BccI CCATC 3 cut(s) 335, 926, 1046
BceAI ACGGC 1 cut(s) 369
BcgI CGANNNNNNTGC 2 cut(s) 379, 413
BciT130I CCWGG 1 cut(s) 1108
BcoDI GTCTC 5 cut(s) 125, 277, 285, 959, 1249
BfaI CTAG 1 cut(s) 1067
BfuAI ACCTGC 1 cut(s) 544
BglII AGATCT 1 cut(s) 215
BisI GCNGC 3 cut(s) 551, 824, 977
BlsI GCNGC 3 cut(s) 552, 825, 978
Bme1390I CCNGG 1 cut(s) 1108
BmeT110I CYCGRG 1 cut(s) 388
BmiI GGNNCC 1 cut(s) 1100
BmrFI CCNGG 1 cut(s) 1108
BmsI GCATC 5 cut(s) 70, 463, 721, 892, 1114
BpiI GAAGAC 3 cut(s) 36, 134, 1055
BpuEI CTTGAG 1 cut(s) 1023
BsaBI GATNNNNATC 2 cut(s) 60, 477
BsaWI WCCGGW 1 cut(s) 753
Bse3DI GCAATG 1 cut(s) 259
Bse8I GATNNNNATC 2 cut(s) 60, 477
BseBI CCWGG 1 cut(s) 1108
BseJI GATNNNNATC 2 cut(s) 60, 477
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 6 cut(s) 443, 479, 619, 688, 741, 866
BseXI GCAGC 3 cut(s) 562, 810, 988
Bsh1236I CGCG 1 cut(s) 1253
BshFI GGCC 1 cut(s) 1225
BsiHKCI CYCGRG 1 cut(s) 388
BsiSI CCGG 1 cut(s) 754
BslFI GGGAC 1 cut(s) 594
BsmAI GTCTC 5 cut(s) 125, 277, 285, 959, 1249
BsmBI CGTCTC 2 cut(s) 125, 285
BsmFI GGGAC 1 cut(s) 594
BsmI GAATGC 1 cut(s) 1290
BsnI GGCC 1 cut(s) 1225
BsoBI CYCGRG 1 cut(s) 388
Bsp143I GATC 7 cut(s) 55, 108, 133, 215, 418, 1020, 1246
BspACI CCGC 1 cut(s) 630
BspANI GGCC 1 cut(s) 1225
BspCNI CTCAG 6 cut(s) 442, 478, 618, 687, 740, 867
BspFNI CGCG 1 cut(s) 1253
BspHI TCATGA 2 cut(s) 520, 1307
BspLI GGNNCC 1 cut(s) 1100
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 1 cut(s) 141
BspQI GCTCTTC 2 cut(s) 252, 874
BsrDI GCAATG 1 cut(s) 259
BssMI GATC 7 cut(s) 55, 108, 133, 215, 418, 1020, 1246
Bst2UI CCWGG 1 cut(s) 1108
Bst4CI ACNGT 4 cut(s) 998, 1061, 1165, 1264
Bst6I CTCTTC 4 cut(s) 252, 275, 874, 975
BstDEI CTNAG 7 cut(s) 305, 429, 465, 605, 674, 727, 875
BstEII GGTNACC 2 cut(s) 935, 1148
BstFNI CGCG 1 cut(s) 1253
BstHHI GCGC 1 cut(s) 779
BstKTI GATC 7 cut(s) 58, 111, 136, 218, 421, 1023, 1249
BstMAI GTCTC 5 cut(s) 125, 277, 285, 959, 1249
BstMBI GATC 7 cut(s) 55, 108, 133, 215, 418, 1020, 1246
BstMWI GCNNNNNNNGC 1 cut(s) 902
BstNI CCWGG 1 cut(s) 1108
BstNSI RCATGY 3 cut(s) 443, 662, 862
BstPI GGTNACC 2 cut(s) 935, 1148
BstSCI CCNGG 1 cut(s) 1106
BstUI CGCG 1 cut(s) 1253
BstV1I GCAGC 3 cut(s) 562, 810, 988
BstV2I GAAGAC 3 cut(s) 36, 134, 1055
BstX2I RGATCY 1 cut(s) 215
BstYI RGATCY 1 cut(s) 215
BsuRI GGCC 1 cut(s) 1225
BtsIMutI CAGTG 3 cut(s) 994, 1180, 1260
BveI ACCTGC 1 cut(s) 544
CciI TCATGA 2 cut(s) 520, 1307
CfoI GCGC 1 cut(s) 779
CseI GACGC 1 cut(s) 302
CsiI ACCWGGT 1 cut(s) 1106
Csp6I GTAC 4 cut(s) 84, 274, 324, 718
CviAII CATG 7 cut(s) 440, 521, 613, 659, 859, 952, 1308
CviQI GTAC 4 cut(s) 84, 274, 324, 718
DdeI CTNAG 7 cut(s) 305, 429, 465, 605, 674, 727, 875
DpnI GATC 7 cut(s) 57, 110, 135, 217, 420, 1022, 1248
DpnII GATC 7 cut(s) 55, 108, 133, 215, 418, 1020, 1246
DraI TTTAAA 1 cut(s) 801
EaeI YGGCCR 1 cut(s) 1223
Eam1104I CTCTTC 4 cut(s) 252, 275, 874, 975
EarI CTCTTC 4 cut(s) 252, 275, 874, 975
Eco32I GATATC 1 cut(s) 15
Eco57I CTGAAG 3 cut(s) 554, 918, 1224
Eco88I CYCGRG 1 cut(s) 388
Eco91I GGTNACC 2 cut(s) 935, 1148
EcoO65I GGTNACC 2 cut(s) 935, 1148
EcoRII CCWGG 1 cut(s) 1106
EcoRV GATATC 1 cut(s) 15
Esp3I CGTCTC 2 cut(s) 125, 285
FaeI CATG 7 cut(s) 443, 524, 616, 662, 862, 955, 1311
FalI AAGNNNNNCTT 2 cut(s) 1273, 1305
FaqI GGGAC 1 cut(s) 594
FatI CATG 7 cut(s) 439, 520, 612, 658, 858, 951, 1307
FauNDI CATATG 3 cut(s) 227, 773, 791
FblI GTMKAC 1 cut(s) 663
Fnu4HI GCNGC 3 cut(s) 551, 824, 977
Fsp4HI GCNGC 3 cut(s) 551, 824, 977
FspBI CTAG 1 cut(s) 1067
GlaI GCGC 1 cut(s) 778
GluI GCNGC 3 cut(s) 551, 824, 977
HaeIII GGCC 1 cut(s) 1225
HapII CCGG 1 cut(s) 754
HgaI GACGC 1 cut(s) 302
HhaI GCGC 1 cut(s) 779
Hin1II CATG 7 cut(s) 443, 524, 616, 662, 862, 955, 1311
Hin6I GCGC 1 cut(s) 777
HinP1I GCGC 1 cut(s) 777
HindIII AAGCTT 1 cut(s) 314
HpaII CCGG 1 cut(s) 754
HphI GGTGA 4 cut(s) 350, 428, 947, 1266
Hpy166II GTNNAC 5 cut(s) 233, 385, 664, 720, 1035
Hpy188I TCNGA 4 cut(s) 108, 730, 1114, 1246
Hpy188III TCNNGA 7 cut(s) 146, 482, 521, 1009, 1067, 1202, 1308
Hpy8I GTNNAC 5 cut(s) 233, 385, 664, 720, 1035
HpyAV CCTTC 2 cut(s) 171, 586
HpyCH4III ACNGT 4 cut(s) 998, 1061, 1165, 1264
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 3 cut(s) 35, 550, 1127
HpyF10VI GCNNNNNNNGC 1 cut(s) 902
HpyF3I CTNAG 7 cut(s) 305, 429, 465, 605, 674, 727, 875
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 7 cut(s) 443, 524, 616, 662, 862, 955, 1311
HspAI GCGC 1 cut(s) 777
Kzo9I GATC 7 cut(s) 55, 108, 133, 215, 418, 1020, 1246
LguI GCTCTTC 2 cut(s) 252, 874
LmnI GCTCC 2 cut(s) 109, 840
Lsp1109I GCAGC 3 cut(s) 562, 810, 988
LweI GCATC 5 cut(s) 70, 463, 721, 892, 1114
MabI ACCWGGT 1 cut(s) 1106
MaeI CTAG 1 cut(s) 1067
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 6 cut(s) 308, 678, 767, 935, 992, 1148
MalI GATC 7 cut(s) 57, 110, 135, 217, 420, 1022, 1248
MboI GATC 7 cut(s) 55, 108, 133, 215, 418, 1020, 1246
MfeI CAATTG 1 cut(s) 1298
MflI RGATCY 1 cut(s) 215
MlsI TGGCCA 1 cut(s) 1225
MluCI AATT 4 cut(s) 268, 446, 1231, 1298
MluNI TGGCCA 1 cut(s) 1225
MlyI GAGTC 5 cut(s) 719, 961, 999, 1030, 1251
MmeI TCCRAC 2 cut(s) 818, 1147
MnlI CCTC 8 cut(s) 3, 384, 465, 528, 647, 838, 1123, 1209
Mox20I TGGCCA 1 cut(s) 1225
MroXI GAANNNNTTC 2 cut(s) 450, 883
MscI TGGCCA 1 cut(s) 1225
MseI TTAA 4 cut(s) 267, 405, 600, 800
MslI CAYNNNNRTG 2 cut(s) 999, 1283
Msp20I TGGCCA 1 cut(s) 1225
MspA1I CMGCKG 1 cut(s) 896
MspI CCGG 1 cut(s) 754
MspR9I CCNGG 1 cut(s) 1108
MunI CAATTG 1 cut(s) 1298
Mva1269I GAATGC 1 cut(s) 1290
MvaI CCWGG 1 cut(s) 1108
MvnI CGCG 1 cut(s) 1253
MwoI GCNNNNNNNGC 1 cut(s) 902
NdeI CATATG 3 cut(s) 227, 773, 791
NdeII GATC 7 cut(s) 55, 108, 133, 215, 418, 1020, 1246
NlaIII CATG 7 cut(s) 443, 524, 616, 662, 862, 955, 1311
NlaIV GGNNCC 1 cut(s) 1100
NmuCI GTSAC 3 cut(s) 678, 935, 992
NspI RCATGY 3 cut(s) 443, 662, 862
PaeR7I CTCGAG 1 cut(s) 388
PagI TCATGA 2 cut(s) 520, 1307
PciI ACATGT 2 cut(s) 439, 658
PciSI GCTCTTC 2 cut(s) 252, 874
PctI GAATGC 1 cut(s) 1290
PdmI GAANNNNTTC 2 cut(s) 450, 883
PfeI GAWTC 7 cut(s) 181, 189, 524, 565, 581, 757, 1170
PkrI GCNGC 3 cut(s) 552, 825, 978
PleI GAGTC 5 cut(s) 719, 961, 999, 1030, 1251
PpsI GAGTC 5 cut(s) 719, 961, 999, 1030, 1251
PscI ACATGT 2 cut(s) 439, 658
PsiI TTATAA 1 cut(s) 1074
Psp6I CCWGG 1 cut(s) 1106
PspEI GGTNACC 2 cut(s) 935, 1148
PspGI CCWGG 1 cut(s) 1106
PspN4I GGNNCC 1 cut(s) 1100
PspXI VCTCGAGB 1 cut(s) 388
PsrI GAACNNNNNNTAC 2 cut(s) 435, 467
PsuI RGATCY 1 cut(s) 215
PvuII CAGCTG 1 cut(s) 896
RsaI GTAC 4 cut(s) 85, 275, 325, 719
RsaNI GTAC 4 cut(s) 84, 274, 324, 718
RseI CAYNNNNRTG 2 cut(s) 999, 1283
SapI GCTCTTC 2 cut(s) 252, 874
SaqAI TTAA 4 cut(s) 267, 405, 600, 800
SatI GCNGC 3 cut(s) 551, 824, 977
Sau3AI GATC 7 cut(s) 55, 108, 133, 215, 418, 1020, 1246
SchI GAGTC 5 cut(s) 719, 961, 999, 1030, 1251
ScrFI CCNGG 1 cut(s) 1108
SexAI ACCWGGT 1 cut(s) 1106
SfaNI GCATC 5 cut(s) 70, 463, 721, 892, 1114
Sfr274I CTCGAG 1 cut(s) 388
SlaI CTCGAG 1 cut(s) 388
SmiMI CAYNNNNRTG 2 cut(s) 999, 1283
SmlI CTYRAG 2 cut(s) 388, 1038
SmoI CTYRAG 2 cut(s) 388, 1038
Sse9I AATT 4 cut(s) 268, 446, 1231, 1298
SsiI CCGC 1 cut(s) 630
SspMI CTAG 1 cut(s) 1067
StyD4I CCNGG 1 cut(s) 1106
TaaI ACNGT 4 cut(s) 998, 1061, 1165, 1264
TaiI ACGT 1 cut(s) 121
TaqI TCGA 5 cut(s) 160, 187, 389, 421, 783
TasI AATT 4 cut(s) 268, 446, 1231, 1298
TatI WGTACW 1 cut(s) 83
TfiI GAWTC 7 cut(s) 181, 189, 524, 565, 581, 757, 1170
Tru1I TTAA 4 cut(s) 267, 405, 600, 800
Tru9I TTAA 4 cut(s) 267, 405, 600, 800
TscAI CASTG 3 cut(s) 1001, 1180, 1267
TseFI GTSAC 3 cut(s) 678, 935, 992
TseI GCWGC 3 cut(s) 550, 823, 976
Tsp45I GTSAC 3 cut(s) 678, 935, 992
TspDTI ATGAA 6 cut(s) 17, 270, 350, 778, 940, 1296
TspGWI ACGGA 2 cut(s) 657, 1149
TspRI CASTG 3 cut(s) 1001, 1180, 1267
XbaI TCTAGA 1 cut(s) 1066
XceI RCATGY 3 cut(s) 443, 662, 862
XhoI CTCGAG 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 663
XmnI GAANNNNTTC 2 cut(s) 450, 883
XspI CTAG 1 cut(s) 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.