MD01G1230100.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006698
Physical Location & Seq
Reverse (-)
31959445 .. 31963061
3617 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1230100.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGACGATGACGAAGACGAAGGGAAGGATTTCGAGAATTGGAAGCTACGCAATTGCGTCTTCCAAACGAGACGGCCACCACCAACAGCCCTGCATTACCTGCACCACTTTCAACATCCTCGCTCCAATTTACAAGCGCCTCAACCATGACGACCCCGATTGTCGGGAAAGCGATTACAGAGCCTATTGGCTGGCCAGGAATCAAAGGATATTGGATTGTTTGTTGGCTGAGAGATCCTCTATCATTTGTCTTCAGGAGTTCTGGGTCGGCAACGAAGAGCTTGTTAACATTTACGAGAAGAGACTTGGGGATGCTGGTTATATCAATCTCAAACTAGGACGAACCAACAACCGCGGCGACGGTTTGCTGACCGCGGTGCATAAAGACTACTTCAGAGTTGTAAACTATGTAGAGTTGCACTTCAATGATTGTGGTGATCGTGTTGCTCAGTTGTTACATGTGGAATTAGCTGCCCCAATTTCGCAGTTTCGAAACAATGATATTCGGCAGGAAATTCTCATTGTGAACACCCACTTGTTATTTCCTCATGACTCGAGTTTGTGTATAGTTCGACTGCATCAGGTCTACAAAATCCTTCAACATGTAGAATCTTATCAGAAAGATAACAAGCTTAATCCCATGCCGATTATACTCTGCGGTGACTGGAATGGAAGCAAAAGTGGACATGTTTACAAGTTTCTTCGGTCCCAGGGGTTCGTATCTTCTTACGATACTGCTCATCATTACACCGATGCAGATGCACATAAGTGGGTTAGCCACAGAAATCATCGGGGAAACATATGCGGCGTTGATTTTATATGGCTTCTCAATCCTAATAGGTACCGTAAGCTACTAAAAACTAGTTGGACCGAAGCAGTATTTGGCATGTTCAAGTACCAACTACGAAGAGCTTCGCTGACAGAGGCTGATGCCTTTGCCTTTCTAAAGGCTGATAATCCTGGTGATTACATAACCTATTCAGGTTTCTGTGAAGCACTTCGACAGCTTAACATGACGGGTCATTGCCATGGACTTAGCCTCGAAGAGACAAAGGAGTTGTGGGTTCAAGCAGACATAGATGGGAACGGCCTTCTTGATAGTATAGAATTTCAGCAGCGAATTTGGTATCCTACGGGGTCAGAGCAGAAGGATGAAAGTGGTAGTGACTGTGACAGTGATTCCAAGGGTATTCAGGAACAAACAATTGGTTTCAGTGTAAAAAACGCGGTGTTCTTCCCTCCTGAAGTGGAGAAAGGAACGTGGCCCAAAGACTACTCTCTTTCCGATCATGCACGACTCACAGTGGTTTTCTCACCAATAAGAATGCCTTGCTCTCAGCTGATTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

450

Amino Acids

51.55

Weight (kDa)

6.83

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 36 - 282 7.8e-09 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 107
Acc65I GGTACC 1 cut(s) 840
AccB1I GGYRCC 1 cut(s) 840
AccI GTMKAC 1 cut(s) 585
AccII CGCG 3 cut(s) 354, 374, 1226
AciI CCGC 7 cut(s) 352, 354, 372, 374, 657, 804, 1226
AclWI GGATC 1 cut(s) 228
AcoI YGGCCR 2 cut(s) 73, 192
AcsI RAATTY 3 cut(s) 513, 1106, 1119
AcuI CTGAAG 3 cut(s) 236, 376, 1263
AfaI GTAC 2 cut(s) 842, 896
AfiI CCNNNNNNNGG 1 cut(s) 162
AflIII ACRYGT 3 cut(s) 457, 601, 685
AgsI TTSAA 5 cut(s) 112, 424, 599, 892, 1067
AhlI ACTAGT 1 cut(s) 860
AjnI CCWGG 3 cut(s) 194, 708, 958
AjuI GAANNNNNNNTTGG 4 cut(s) 864, 896, 1188, 1220
AleI CACNNNNGTG 1 cut(s) 766
AluBI AGCT 8 cut(s) 45, 280, 470, 631, 850, 911, 1006, 1339
AluI AGCT 8 cut(s) 45, 280, 470, 631, 850, 911, 1006, 1339
Alw26I GTCTC 3 cut(s) 63, 295, 1040
AlwI GGATC 1 cut(s) 228
AlwNI CAGNNNCTG 1 cut(s) 926
Ama87I CYCGRG 1 cut(s) 553
AoxI GGCC 4 cut(s) 73, 192, 1087, 1262
ApeKI GCWGC 2 cut(s) 470, 1114
ApoI RAATTY 3 cut(s) 513, 1106, 1119
Asp700I GAANNNNTTC 3 cut(s) 28, 910, 996
Asp718I GGTACC 1 cut(s) 840
AspLEI GCGC 1 cut(s) 138
AspS9I GGNCC 3 cut(s) 705, 867, 1263
AsuHPI GGTGA 4 cut(s) 446, 671, 974, 1305
AsuII TTCGAA 1 cut(s) 490
AvaI CYCGRG 1 cut(s) 553
AvaII GGWCC 2 cut(s) 705, 867
BalI TGGCCA 1 cut(s) 194
BanI GGYRCC 1 cut(s) 840
BbsI GAAGAC 3 cut(s) 20, 51, 242
BbvI GCAGC 2 cut(s) 457, 1126
BccI CCATC 1 cut(s) 1073
BceAI ACGGC 2 cut(s) 88, 1102
BcgI CGANNNNNNTGC 2 cut(s) 740, 774
BciT130I CCWGG 3 cut(s) 196, 710, 960
BciVI GTATCC 1 cut(s) 1137
BcoDI GTCTC 3 cut(s) 63, 295, 1040
BcuI ACTAGT 1 cut(s) 860
BfaI CTAG 2 cut(s) 335, 861
BfoI RGCGCY 1 cut(s) 139
BfuAI ACCTGC 1 cut(s) 107
BfuI GTATCC 1 cut(s) 1137
BisI GCNGC 4 cut(s) 355, 471, 805, 1115
BlsI GCNGC 4 cut(s) 356, 472, 806, 1116
Bme1390I CCNGG 3 cut(s) 196, 710, 960
Bme18I GGWCC 2 cut(s) 705, 867
BmeT110I CYCGRG 1 cut(s) 553
BmgT120I GGNCC 3 cut(s) 705, 867, 1263
BmiI GGNNCC 2 cut(s) 707, 842
BmrFI CCNGG 3 cut(s) 196, 710, 960
BmsI GCATC 5 cut(s) 301, 586, 742, 748, 919
BpiI GAAGAC 3 cut(s) 20, 51, 242
BplI GAGNNNNNCTC 2 cut(s) 221, 253
Bpu14I TTCGAA 1 cut(s) 490
BsaJI CCNNGG 6 cut(s) 352, 372, 708, 709, 1027, 1182
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse1I ACTGG 1 cut(s) 668
Bse3DI GCAATG 1 cut(s) 1021
BseBI CCWGG 3 cut(s) 196, 710, 960
BseDI CCNNGG 6 cut(s) 352, 372, 708, 709, 1027, 1182
BseGI GGATG 3 cut(s) 114, 316, 1156
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMI GCAATG 1 cut(s) 1021
BseMII CTCAG 3 cut(s) 219, 461, 1349
BseNI ACTGG 1 cut(s) 668
BseXI GCAGC 2 cut(s) 457, 1126
BsgI GTGCAG 1 cut(s) 85
Bsh1236I CGCG 3 cut(s) 354, 374, 1226
BshFI GGCC 4 cut(s) 75, 194, 1089, 1264
BshNI GGYRCC 1 cut(s) 840
BsiHKCI CYCGRG 1 cut(s) 553
BslFI GGGAC 1 cut(s) 691
BslI CCNNNNNNNGG 1 cut(s) 162
BsmAI GTCTC 3 cut(s) 63, 295, 1040
BsmBI CGTCTC 1 cut(s) 63
BsmFI GGGAC 1 cut(s) 691
BsmI GAATGC 1 cut(s) 1329
BsnI GGCC 4 cut(s) 75, 194, 1089, 1264
BsoBI CYCGRG 1 cut(s) 553
Bsp119I TTCGAA 1 cut(s) 490
Bsp143I GATC 3 cut(s) 233, 436, 1285
Bsp19I CCATGG 1 cut(s) 1027
BspACI CCGC 7 cut(s) 352, 354, 372, 374, 657, 804, 1226
BspANI GGCC 4 cut(s) 75, 194, 1089, 1264
BspCNI CTCAG 3 cut(s) 220, 460, 1348
BspFNI CGCG 3 cut(s) 354, 374, 1226
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 2 cut(s) 707, 842
BspMI ACCTGC 1 cut(s) 107
BspPI GGATC 1 cut(s) 228
BspQI GCTCTTC 2 cut(s) 270, 901
BspT104I TTCGAA 1 cut(s) 490
BspT107I GGYRCC 1 cut(s) 840
BsrDI GCAATG 1 cut(s) 1021
BsrI ACTGG 1 cut(s) 668
BssECI CCNNGG 6 cut(s) 352, 372, 708, 709, 1027, 1182
BssMI GATC 3 cut(s) 233, 436, 1285
BssT1I CCWWGG 2 cut(s) 1027, 1182
Bst2UI CCWGG 3 cut(s) 196, 710, 960
Bst4CI ACNGT 5 cut(s) 362, 845, 1169, 1175, 1303
Bst6I CTCTTC 4 cut(s) 270, 293, 901, 1038
BstAPI GCANNNNNTGC 1 cut(s) 99
BstBI TTCGAA 1 cut(s) 490
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 4 cut(s) 228, 447, 1034, 1335
BstDSI CCRYGG 3 cut(s) 352, 372, 1027
BstF5I GGATG 3 cut(s) 114, 316, 1156
BstFNI CGCG 3 cut(s) 354, 374, 1226
BstH2I RGCGCY 1 cut(s) 139
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 3 cut(s) 236, 439, 1288
BstMAI GTCTC 3 cut(s) 63, 295, 1040
BstMBI GATC 3 cut(s) 233, 436, 1285
BstMWI GCNNNNNNNGC 1 cut(s) 99
BstNI CCWGG 3 cut(s) 196, 710, 960
BstNSI RCATGY 4 cut(s) 461, 605, 689, 889
BstSCI CCNGG 3 cut(s) 194, 708, 958
BstUI CGCG 3 cut(s) 354, 374, 1226
BstV1I GCAGC 2 cut(s) 457, 1126
BstV2I GAAGAC 3 cut(s) 20, 51, 242
BstX2I RGATCY 1 cut(s) 233
BstYI RGATCY 1 cut(s) 233
BsuI GTATCC 1 cut(s) 1137
BsuRI GGCC 4 cut(s) 75, 194, 1089, 1264
BtgI CCRYGG 3 cut(s) 352, 372, 1027
BtsCI GGATG 3 cut(s) 114, 316, 1156
BtsIMutI CAGTG 3 cut(s) 1180, 1219, 1308
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 1 cut(s) 192
CaiI CAGNNNCTG 1 cut(s) 926
CciI TCATGA 1 cut(s) 547
CfoI GCGC 1 cut(s) 138
Cfr13I GGNCC 3 cut(s) 705, 867, 1263
Cfr42I CCGCGG 2 cut(s) 355, 375
CseI GACGC 1 cut(s) 45
Csp6I GTAC 2 cut(s) 841, 895
CspCI CAANNNNNGTGG 2 cut(s) 412, 447
CviQI GTAC 2 cut(s) 841, 895
DdeI CTNAG 4 cut(s) 228, 447, 1034, 1335
DpnI GATC 3 cut(s) 235, 438, 1287
DpnII GATC 3 cut(s) 233, 436, 1285
EaeI YGGCCR 2 cut(s) 73, 192
Eam1104I CTCTTC 4 cut(s) 270, 293, 901, 1038
EarI CTCTTC 4 cut(s) 270, 293, 901, 1038
Eco130I CCWWGG 2 cut(s) 1027, 1182
Eco47I GGWCC 2 cut(s) 705, 867
Eco57I CTGAAG 3 cut(s) 236, 376, 1263
Eco88I CYCGRG 1 cut(s) 553
EcoRII CCWGG 3 cut(s) 194, 708, 958
EcoT14I CCWWGG 2 cut(s) 1027, 1182
ErhI CCWWGG 2 cut(s) 1027, 1182
Esp3I CGTCTC 1 cut(s) 63
FalI AAGNNNNNCTT 2 cut(s) 1312, 1344
FaqI GGGAC 1 cut(s) 691
FauNDI CATATG 1 cut(s) 800
FblI GTMKAC 1 cut(s) 585
Fnu4HI GCNGC 4 cut(s) 355, 471, 805, 1115
FokI GGATG 3 cut(s) 101, 323, 1163
Fsp4HI GCNGC 4 cut(s) 355, 471, 805, 1115
FspBI CTAG 2 cut(s) 335, 861
GlaI GCGC 1 cut(s) 137
GluI GCNGC 4 cut(s) 355, 471, 805, 1115
HaeII RGCGCY 1 cut(s) 139
HaeIII GGCC 4 cut(s) 75, 194, 1089, 1264
HgaI GACGC 1 cut(s) 45
HhaI GCGC 1 cut(s) 138
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HincII GTYRAC 1 cut(s) 286
HindII GTYRAC 1 cut(s) 286
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 6 cut(s) 199, 551, 608, 1178, 1296, 1342
HpaI GTTAAC 1 cut(s) 286
HphI GGTGA 4 cut(s) 446, 671, 974, 1305
Hpy166II GTNNAC 6 cut(s) 286, 403, 526, 586, 683, 691
Hpy188I TCNGA 4 cut(s) 395, 618, 1141, 1285
Hpy188III TCNNGA 7 cut(s) 33, 164, 254, 548, 1094, 1193, 1241
Hpy8I GTNNAC 6 cut(s) 286, 403, 526, 586, 683, 691
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 5 cut(s) 13, 18, 605, 1100, 1141
HpyCH4III ACNGT 5 cut(s) 362, 845, 1169, 1175, 1303
HpyCH4IV ACGT 1 cut(s) 1259
HpyCH4V TGCA 8 cut(s) 93, 102, 379, 418, 577, 755, 761, 1292
HpyF10VI GCNNNNNNNGC 1 cut(s) 99
HpyF3I CTNAG 4 cut(s) 228, 447, 1034, 1335
HpySE526I ACGT 1 cut(s) 1259
HspAI GCGC 1 cut(s) 136
KpnI GGTACC 1 cut(s) 844
KspAI GTTAAC 1 cut(s) 286
KspI CCGCGG 2 cut(s) 355, 375
Kzo9I GATC 3 cut(s) 233, 436, 1285
LguI GCTCTTC 2 cut(s) 270, 901
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 2 cut(s) 457, 1126
LweI GCATC 5 cut(s) 301, 586, 742, 748, 919
MaeI CTAG 2 cut(s) 335, 861
MaeII ACGT 1 cut(s) 1259
MaeIII GTNAC 4 cut(s) 453, 659, 1163, 1169
MalI GATC 3 cut(s) 235, 438, 1287
MboI GATC 3 cut(s) 233, 436, 1285
MfeI CAATTG 2 cut(s) 51, 1203
MflI RGATCY 1 cut(s) 233
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 9 cut(s) 36, 51, 126, 464, 477, 513, 1106, 1119, 1203
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 2 cut(s) 545, 1290
MmeI TCCRAC 1 cut(s) 845
MnlI CCTC 7 cut(s) 128, 149, 247, 555, 916, 1049, 1248
Mox20I TGGCCA 1 cut(s) 194
MroXI GAANNNNTTC 3 cut(s) 28, 910, 996
MscI TGGCCA 1 cut(s) 194
MseI TTAA 3 cut(s) 285, 633, 1008
MslI CAYNNNNRTG 3 cut(s) 423, 766, 1026
Msp20I TGGCCA 1 cut(s) 194
MspA1I CMGCKG 3 cut(s) 354, 374, 1339
MspR9I CCNGG 3 cut(s) 196, 710, 960
MunI CAATTG 2 cut(s) 51, 1203
Mva1269I GAATGC 1 cut(s) 1329
MvaI CCWGG 3 cut(s) 196, 710, 960
MvnI CGCG 3 cut(s) 354, 374, 1226
MwoI GCNNNNNNNGC 1 cut(s) 99
NcoI CCATGG 1 cut(s) 1027
NdeI CATATG 1 cut(s) 800
NdeII GATC 3 cut(s) 233, 436, 1285
NlaIV GGNNCC 2 cut(s) 707, 842
NmuCI GTSAC 3 cut(s) 659, 1163, 1169
NspI RCATGY 4 cut(s) 461, 605, 689, 889
NspV TTCGAA 1 cut(s) 490
OliI CACNNNNGTG 1 cut(s) 766
PaeR7I CTCGAG 1 cut(s) 553
PagI TCATGA 1 cut(s) 547
PasI CCCWGGG 1 cut(s) 709
PciI ACATGT 3 cut(s) 457, 601, 685
PciSI GCTCTTC 2 cut(s) 270, 901
PctI GAATGC 1 cut(s) 1329
PdmI GAANNNNTTC 3 cut(s) 28, 910, 996
PfeI GAWTC 4 cut(s) 199, 608, 1178, 1342
PkrI GCNGC 4 cut(s) 356, 472, 806, 1116
PleI GAGTC 2 cut(s) 545, 1290
PpsI GAGTC 2 cut(s) 545, 1290
PscI ACATGT 3 cut(s) 457, 601, 685
Psp6I CCWGG 3 cut(s) 194, 708, 958
PspGI CCWGG 3 cut(s) 194, 708, 958
PspN4I GGNNCC 2 cut(s) 707, 842
PspPI GGNCC 3 cut(s) 705, 867, 1263
PspXI VCTCGAGB 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 926
PsuI RGATCY 1 cut(s) 233
PvuII CAGCTG 1 cut(s) 1339
RsaI GTAC 2 cut(s) 842, 896
RsaNI GTAC 2 cut(s) 841, 895
RseI CAYNNNNRTG 3 cut(s) 423, 766, 1026
SacII CCGCGG 2 cut(s) 355, 375
SapI GCTCTTC 2 cut(s) 270, 901
SaqAI TTAA 3 cut(s) 285, 633, 1008
SatI GCNGC 4 cut(s) 355, 471, 805, 1115
Sau3AI GATC 3 cut(s) 233, 436, 1285
Sau96I GGNCC 3 cut(s) 705, 867, 1263
SchI GAGTC 2 cut(s) 545, 1290
ScrFI CCNGG 3 cut(s) 196, 710, 960
SfaNI GCATC 5 cut(s) 301, 586, 742, 748, 919
Sfr274I CTCGAG 1 cut(s) 553
Sfr303I CCGCGG 2 cut(s) 355, 375
SfuI TTCGAA 1 cut(s) 490
SgrBI CCGCGG 2 cut(s) 355, 375
SinI GGWCC 2 cut(s) 705, 867
SlaI CTCGAG 1 cut(s) 553
SmiMI CAYNNNNRTG 3 cut(s) 423, 766, 1026
SmlI CTYRAG 1 cut(s) 553
SmoI CTYRAG 1 cut(s) 553
SpeI ACTAGT 1 cut(s) 860
Sse9I AATT 9 cut(s) 36, 51, 126, 464, 477, 513, 1106, 1119, 1203
SsiI CCGC 7 cut(s) 352, 354, 372, 374, 657, 804, 1226
SspMI CTAG 2 cut(s) 335, 861
StyD4I CCNGG 3 cut(s) 194, 708, 958
StyI CCWWGG 2 cut(s) 1027, 1182
TaaI ACNGT 5 cut(s) 362, 845, 1169, 1175, 1303
TaiI ACGT 1 cut(s) 1262
TaqI TCGA 6 cut(s) 32, 490, 554, 571, 1000, 1041
TaqII GACCGA 2 cut(s) 693, 884
TasI AATT 9 cut(s) 36, 51, 126, 464, 477, 513, 1106, 1119, 1203
TauI GCSGC 2 cut(s) 357, 807
TfiI GAWTC 4 cut(s) 199, 608, 1178, 1342
Tru1I TTAA 3 cut(s) 285, 633, 1008
Tru9I TTAA 3 cut(s) 285, 633, 1008
TscAI CASTG 3 cut(s) 1180, 1219, 1308
TseFI GTSAC 3 cut(s) 659, 1163, 1169
TseI GCWGC 2 cut(s) 470, 1114
Tsp45I GTSAC 3 cut(s) 659, 1163, 1169
TspDTI ATGAA 1 cut(s) 1167
TspRI CASTG 3 cut(s) 1180, 1219, 1308
VpaK11BI GGWCC 2 cut(s) 705, 867
XapI RAATTY 3 cut(s) 513, 1106, 1119
XceI RCATGY 4 cut(s) 461, 605, 689, 889
XhoI CTCGAG 1 cut(s) 553
XmiI GTMKAC 1 cut(s) 585
XmnI GAANNNNTTC 3 cut(s) 28, 910, 996
XspI CTAG 2 cut(s) 335, 861
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.