MD07G1301900.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006698
Physical Location & Seq
Reverse (-)
36046122 .. 36050017
3896 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1301900.v1.1.491

Sequence Viewer

Length: 1332 bp
ATGACGAAGACGAAGGGAAGGATATCGAGAATTGGAAGCTATGCAATTGCGTCTTCCAAACTAGACGGCCACCACCAACAGCCATGCATTACCTGCACCACTTTCAACATCCTCGCTCCCATTTACAAGCGCCTCAACCATGACGATCCTGATAGTCGAGAAAGCGATCACAGAGCGTATTGGCTGGCCAGGAACCAAAGGATACTGGATTGTTTGTTGGCTGAGAGATCTTCCATCATTTGTCTTCAGGAGTTCTGGGTCGGAAACGAAGAGCTTGTGAACATTTACGAGAAGAAACTTGGAGATGCTGGTTATATCAATCTCAAACTAGGACGAACCAACAACCGTGGCGATGGTTTGCTGACCGCGGTGCATAAGGACTACTTTAGAGTTGTAAACTATGAAGAGTTGCACTTCAATGATTATGGTGATCGTGTTGCTCAGTTGATACATGTGGAATTAGCTACTTTCCGAAACAACGACATTCGACAAGAAATTCTCATCGTGAACACCCACTTGTTATTTCCTCATGACTCGAGTTTGTGTATAGTTCGATTGCATCAGGTCTACAAAATCTTGCAACATTTAGAATCTTATCAGAAAGAGAACAAGCTTAATCCCATGCCGATTATTCTCTGCGGTGACTGGAACGGAAGCAAACGTGGACATGTTTACGAGTTTCTTCGGTCCCAGGGGTTTGTATCTTCTTATGATACTGCTCATCATTATACCGATGCAGATGCACACAAGTGGGTTAGCCACAGAAACCATAGGGGAAACATATGCGGCGTTGATTTTATATGGCTTCTCAATCCTAATAGATATCGTAAGCTACTAAAAACTAGTTGGACTGAAGCAGTATTTGGAATGTTCAAGTATCTACTACGAAGAGCTTCGCTGACAGCGGATGATGCCTTTGCTTTTCTGAAGACTGATAATCCTGTTGATTACATTACCTATTCAGGTTTCTGTGAAGCACTTCGACAGCTTAACATGACAGGTCATTGCCATGGACTTAGCCACGACGAGACAAAGGATTTGTGGGTTCAAGCAGACATAGATGGAAATGGTTTTCTTGATTACATAGAATTTCAGCAGCGAATTTGGAATCCTACGGGGTCAGAGCAGAAAGATGAAAGCGGTAGTGACTGTGATAGTGATTCCAAGGGTATTCAGGAACAAACAATTGGTTTCAGTGTCAAAAACGCTGTTCTCTTCCCTCCTGAAGTGGAGAAAGGAACGTGGCCCGAAGACTACTCTCTTTCTGATCATGCACGACTCACTGTGGTTTTTTCACCAATAAGAATGCCTTGCTCTCAGATGATTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

444

Amino Acids

51.1

Weight (kDa)

6.31

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 34 - 316 1.8e-09 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 101
AccI GTMKAC 1 cut(s) 567
AccII CGCG 1 cut(s) 368
AciI CCGC 6 cut(s) 366, 368, 639, 786, 905, 1140
AclWI GGATC 1 cut(s) 140
AcoI YGGCCR 2 cut(s) 67, 186
AcsI RAATTY 3 cut(s) 495, 1088, 1101
AcuI CTGAAG 4 cut(s) 230, 873, 947, 1245
AflIII ACRYGT 2 cut(s) 451, 667
AgsI TTSAA 4 cut(s) 106, 418, 874, 1049
AhlI ACTAGT 1 cut(s) 842
AjnI CCWGG 2 cut(s) 188, 690
AjuI GAANNNNNNNTTGG 4 cut(s) 846, 878, 1170, 1202
AleI CACNNNNGTG 1 cut(s) 748
AluBI AGCT 7 cut(s) 39, 274, 464, 613, 832, 893, 988
AluI AGCT 7 cut(s) 39, 274, 464, 613, 832, 893, 988
Alw26I GTCTC 1 cut(s) 1022
AlwI GGATC 1 cut(s) 140
Ama87I CYCGRG 1 cut(s) 535
AoxI GGCC 3 cut(s) 67, 186, 1244
ApeKI GCWGC 1 cut(s) 1096
ApoI RAATTY 3 cut(s) 495, 1088, 1101
ArsI GACNNNNNNTTYG 2 cut(s) 1021, 1053
Asp700I GAANNNNTTC 2 cut(s) 892, 978
AspLEI GCGC 1 cut(s) 132
AspS9I GGNCC 2 cut(s) 687, 1245
AsuHPI GGTGA 3 cut(s) 440, 653, 1287
AvaI CYCGRG 1 cut(s) 535
AvaII GGWCC 1 cut(s) 687
BalI TGGCCA 1 cut(s) 188
BbsI GAAGAC 5 cut(s) 14, 45, 236, 935, 1257
BbvI GCAGC 1 cut(s) 1108
BccI CCATC 3 cut(s) 242, 347, 1055
BceAI ACGGC 1 cut(s) 82
BcgI CGANNNNNNTGC 2 cut(s) 722, 756
BciT130I CCWGG 2 cut(s) 190, 692
BciVI GTATCC 1 cut(s) 195
BclI TGATCA 1 cut(s) 1267
BcoDI GTCTC 1 cut(s) 1022
BcuI ACTAGT 1 cut(s) 842
BfaI CTAG 3 cut(s) 62, 329, 843
BfoI RGCGCY 1 cut(s) 133
BfuAI ACCTGC 1 cut(s) 101
BfuI GTATCC 1 cut(s) 195
BglII AGATCT 1 cut(s) 227
BisI GCNGC 2 cut(s) 787, 1097
BlsI GCNGC 2 cut(s) 788, 1098
Bme1390I CCNGG 2 cut(s) 190, 692
Bme18I GGWCC 1 cut(s) 687
BmeT110I CYCGRG 1 cut(s) 535
BmgT120I GGNCC 2 cut(s) 687, 1245
BmiI GGNNCC 2 cut(s) 194, 689
BmrFI CCNGG 2 cut(s) 190, 692
BmsI GCATC 5 cut(s) 295, 568, 724, 730, 901
BpiI GAAGAC 5 cut(s) 14, 45, 236, 935, 1257
BsaJI CCNNGG 6 cut(s) 346, 366, 690, 691, 1009, 1164
Bse1I ACTGG 2 cut(s) 210, 650
Bse3DI GCAATG 1 cut(s) 1003
BseBI CCWGG 2 cut(s) 190, 692
BseDI CCNNGG 6 cut(s) 346, 366, 690, 691, 1009, 1164
BseGI GGATG 2 cut(s) 108, 913
BseMI GCAATG 1 cut(s) 1003
BseMII CTCAG 3 cut(s) 213, 455, 1331
BseNI ACTGG 2 cut(s) 210, 650
BseXI GCAGC 1 cut(s) 1108
BsgI GTGCAG 1 cut(s) 79
Bsh1236I CGCG 1 cut(s) 368
BshFI GGCC 3 cut(s) 69, 188, 1246
BsiHKCI CYCGRG 1 cut(s) 535
BslFI GGGAC 1 cut(s) 673
BsmAI GTCTC 1 cut(s) 1022
BsmFI GGGAC 1 cut(s) 673
BsmI GAATGC 1 cut(s) 1311
BsnI GGCC 3 cut(s) 69, 188, 1246
BsoBI CYCGRG 1 cut(s) 535
Bsp143I GATC 5 cut(s) 145, 166, 227, 430, 1267
Bsp19I CCATGG 1 cut(s) 1009
BspACI CCGC 6 cut(s) 366, 368, 639, 786, 905, 1140
BspANI GGCC 3 cut(s) 69, 188, 1246
BspCNI CTCAG 3 cut(s) 214, 454, 1330
BspFNI CGCG 1 cut(s) 368
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 2 cut(s) 194, 689
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 1 cut(s) 140
BspQI GCTCTTC 2 cut(s) 264, 883
BsrDI GCAATG 1 cut(s) 1003
BsrI ACTGG 2 cut(s) 210, 650
BssECI CCNNGG 6 cut(s) 346, 366, 690, 691, 1009, 1164
BssMI GATC 5 cut(s) 145, 166, 227, 430, 1267
BssT1I CCWWGG 2 cut(s) 1009, 1164
Bst2UI CCWGG 2 cut(s) 190, 692
Bst4CI ACNGT 3 cut(s) 347, 1151, 1285
Bst6I CTCTTC 4 cut(s) 264, 399, 883, 1220
BstAPI GCANNNNNTGC 1 cut(s) 93
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 4 cut(s) 222, 441, 1016, 1317
BstDSI CCRYGG 3 cut(s) 346, 366, 1009
BstF5I GGATG 2 cut(s) 108, 913
BstFNI CGCG 1 cut(s) 368
BstH2I RGCGCY 1 cut(s) 133
BstHHI GCGC 1 cut(s) 132
BstKTI GATC 5 cut(s) 148, 169, 230, 433, 1270
BstMAI GTCTC 1 cut(s) 1022
BstMBI GATC 5 cut(s) 145, 166, 227, 430, 1267
BstMWI GCNNNNNNNGC 2 cut(s) 93, 911
BstNI CCWGG 2 cut(s) 190, 692
BstNSI RCATGY 2 cut(s) 455, 671
BstSCI CCNGG 2 cut(s) 188, 690
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 1 cut(s) 1108
BstV2I GAAGAC 5 cut(s) 14, 45, 236, 935, 1257
BstX2I RGATCY 1 cut(s) 227
BstYI RGATCY 1 cut(s) 227
BsuI GTATCC 1 cut(s) 195
BsuRI GGCC 3 cut(s) 69, 188, 1246
BtgI CCRYGG 3 cut(s) 346, 366, 1009
BtgZI GCGATG 1 cut(s) 366
BtsCI GGATG 2 cut(s) 108, 913
BtsIMutI CAGTG 2 cut(s) 1201, 1281
BveI ACCTGC 1 cut(s) 101
Cac8I GCNNGC 1 cut(s) 186
CciI TCATGA 1 cut(s) 529
CfoI GCGC 1 cut(s) 132
Cfr13I GGNCC 2 cut(s) 687, 1245
Cfr42I CCGCGG 1 cut(s) 369
CseI GACGC 1 cut(s) 39
CspCI CAANNNNNGTGG 2 cut(s) 328, 363
CviAII CATG 9 cut(s) 84, 140, 452, 530, 622, 668, 994, 1010, 1271
DdeI CTNAG 4 cut(s) 222, 441, 1016, 1317
DpnI GATC 5 cut(s) 147, 168, 229, 432, 1269
DpnII GATC 5 cut(s) 145, 166, 227, 430, 1267
EaeI YGGCCR 2 cut(s) 67, 186
Eam1104I CTCTTC 4 cut(s) 264, 399, 883, 1220
EarI CTCTTC 4 cut(s) 264, 399, 883, 1220
Eco130I CCWWGG 2 cut(s) 1009, 1164
Eco32I GATATC 2 cut(s) 24, 824
Eco47I GGWCC 1 cut(s) 687
Eco57I CTGAAG 4 cut(s) 230, 873, 947, 1245
Eco88I CYCGRG 1 cut(s) 535
EcoRII CCWGG 2 cut(s) 188, 690
EcoRV GATATC 2 cut(s) 24, 824
EcoT14I CCWWGG 2 cut(s) 1009, 1164
EcoT22I ATGCAT 1 cut(s) 89
ErhI CCWWGG 2 cut(s) 1009, 1164
FaeI CATG 9 cut(s) 87, 143, 455, 533, 625, 671, 997, 1013, 1274
FalI AAGNNNNNCTT 4 cut(s) 368, 400, 1294, 1326
FaqI GGGAC 1 cut(s) 673
FatI CATG 9 cut(s) 83, 139, 451, 529, 621, 667, 993, 1009, 1270
FauNDI CATATG 1 cut(s) 782
FbaI TGATCA 1 cut(s) 1267
FblI GTMKAC 1 cut(s) 567
Fnu4HI GCNGC 2 cut(s) 787, 1097
FokI GGATG 2 cut(s) 95, 920
Fsp4HI GCNGC 2 cut(s) 787, 1097
FspBI CTAG 3 cut(s) 62, 329, 843
GlaI GCGC 1 cut(s) 131
GluI GCNGC 2 cut(s) 787, 1097
HaeII RGCGCY 1 cut(s) 133
HaeIII GGCC 3 cut(s) 69, 188, 1246
HgaI GACGC 1 cut(s) 39
HhaI GCGC 1 cut(s) 132
Hin1II CATG 9 cut(s) 87, 143, 455, 533, 625, 671, 997, 1013, 1274
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 6 cut(s) 533, 590, 1108, 1160, 1278, 1324
HphI GGTGA 3 cut(s) 440, 653, 1287
Hpy166II GTNNAC 6 cut(s) 280, 397, 508, 568, 665, 673
Hpy188I TCNGA 8 cut(s) 263, 473, 600, 927, 1123, 1267, 1320, 1331
Hpy188III TCNNGA 9 cut(s) 27, 149, 158, 248, 505, 530, 1076, 1175, 1223
Hpy8I GTNNAC 6 cut(s) 280, 397, 508, 568, 665, 673
Hpy99I CGWCG 1 cut(s) 1028
HpyAV CCTTC 2 cut(s) 7, 12
HpyCH4III ACNGT 3 cut(s) 347, 1151, 1285
HpyCH4IV ACGT 2 cut(s) 661, 1241
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 911
HpyF3I CTNAG 4 cut(s) 222, 441, 1016, 1317
HpySE526I ACGT 2 cut(s) 661, 1241
Hsp92II CATG 9 cut(s) 87, 143, 455, 533, 625, 671, 997, 1013, 1274
HspAI GCGC 1 cut(s) 130
Ksp22I TGATCA 1 cut(s) 1267
KspI CCGCGG 1 cut(s) 369
Kzo9I GATC 5 cut(s) 145, 166, 227, 430, 1267
LguI GCTCTTC 2 cut(s) 264, 883
LmnI GCTCC 1 cut(s) 121
Lsp1109I GCAGC 1 cut(s) 1108
LweI GCATC 5 cut(s) 295, 568, 724, 730, 901
MaeI CTAG 3 cut(s) 62, 329, 843
MaeII ACGT 2 cut(s) 661, 1241
MaeIII GTNAC 2 cut(s) 641, 1145
MalI GATC 5 cut(s) 147, 168, 229, 432, 1269
MboI GATC 5 cut(s) 145, 166, 227, 430, 1267
MfeI CAATTG 2 cut(s) 45, 1185
MflI RGATCY 1 cut(s) 227
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 7 cut(s) 30, 45, 458, 495, 1088, 1101, 1185
MluNI TGGCCA 1 cut(s) 188
MlyI GAGTC 2 cut(s) 527, 1272
MmeI TCCRAC 2 cut(s) 241, 827
MnlI CCTC 4 cut(s) 122, 143, 537, 1230
Mox20I TGGCCA 1 cut(s) 188
Mph1103I ATGCAT 1 cut(s) 89
MroXI GAANNNNTTC 2 cut(s) 892, 978
MscI TGGCCA 1 cut(s) 188
MseI TTAA 2 cut(s) 615, 990
MslI CAYNNNNRTG 3 cut(s) 417, 748, 1008
Msp20I TGGCCA 1 cut(s) 188
MspA1I CMGCKG 2 cut(s) 368, 905
MspR9I CCNGG 2 cut(s) 190, 692
MunI CAATTG 2 cut(s) 45, 1185
Mva1269I GAATGC 1 cut(s) 1311
MvaI CCWGG 2 cut(s) 190, 692
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 2 cut(s) 93, 911
NcoI CCATGG 1 cut(s) 1009
NdeI CATATG 1 cut(s) 782
NdeII GATC 5 cut(s) 145, 166, 227, 430, 1267
NlaIII CATG 9 cut(s) 87, 143, 455, 533, 625, 671, 997, 1013, 1274
NlaIV GGNNCC 2 cut(s) 194, 689
NmuCI GTSAC 2 cut(s) 641, 1145
NsiI ATGCAT 1 cut(s) 89
NspI RCATGY 2 cut(s) 455, 671
OliI CACNNNNGTG 1 cut(s) 748
PaeR7I CTCGAG 1 cut(s) 535
PagI TCATGA 1 cut(s) 529
PasI CCCWGGG 1 cut(s) 691
PciI ACATGT 2 cut(s) 451, 667
PciSI GCTCTTC 2 cut(s) 264, 883
PctI GAATGC 1 cut(s) 1311
PdmI GAANNNNTTC 2 cut(s) 892, 978
PfeI GAWTC 4 cut(s) 590, 1108, 1160, 1324
PkrI GCNGC 2 cut(s) 788, 1098
PleI GAGTC 2 cut(s) 527, 1272
PpsI GAGTC 2 cut(s) 527, 1272
PscI ACATGT 2 cut(s) 451, 667
Psp6I CCWGG 2 cut(s) 188, 690
PspGI CCWGG 2 cut(s) 188, 690
PspN4I GGNNCC 2 cut(s) 194, 689
PspPI GGNCC 2 cut(s) 687, 1245
PspXI VCTCGAGB 1 cut(s) 535
PsuI RGATCY 1 cut(s) 227
RseI CAYNNNNRTG 3 cut(s) 417, 748, 1008
SacII CCGCGG 1 cut(s) 369
SapI GCTCTTC 2 cut(s) 264, 883
SaqAI TTAA 2 cut(s) 615, 990
SatI GCNGC 2 cut(s) 787, 1097
Sau3AI GATC 5 cut(s) 145, 166, 227, 430, 1267
Sau96I GGNCC 2 cut(s) 687, 1245
SchI GAGTC 2 cut(s) 527, 1272
ScrFI CCNGG 2 cut(s) 190, 692
SfaNI GCATC 5 cut(s) 295, 568, 724, 730, 901
Sfr274I CTCGAG 1 cut(s) 535
Sfr303I CCGCGG 1 cut(s) 369
SgrBI CCGCGG 1 cut(s) 369
SinI GGWCC 1 cut(s) 687
SlaI CTCGAG 1 cut(s) 535
SmiMI CAYNNNNRTG 3 cut(s) 417, 748, 1008
SmlI CTYRAG 1 cut(s) 535
SmoI CTYRAG 1 cut(s) 535
SpeI ACTAGT 1 cut(s) 842
Sse9I AATT 7 cut(s) 30, 45, 458, 495, 1088, 1101, 1185
SsiI CCGC 6 cut(s) 366, 368, 639, 786, 905, 1140
SspMI CTAG 3 cut(s) 62, 329, 843
StyD4I CCNGG 2 cut(s) 188, 690
StyI CCWWGG 2 cut(s) 1009, 1164
TaaI ACNGT 3 cut(s) 347, 1151, 1285
TaiI ACGT 2 cut(s) 664, 1244
TaqI TCGA 6 cut(s) 26, 157, 487, 536, 553, 982
TaqII GACCGA 1 cut(s) 675
TasI AATT 7 cut(s) 30, 45, 458, 495, 1088, 1101, 1185
TauI GCSGC 1 cut(s) 789
TfiI GAWTC 4 cut(s) 590, 1108, 1160, 1324
Tru1I TTAA 2 cut(s) 615, 990
Tru9I TTAA 2 cut(s) 615, 990
TscAI CASTG 2 cut(s) 1201, 1288
TseFI GTSAC 2 cut(s) 641, 1145
TseI GCWGC 1 cut(s) 1096
Tsp45I GTSAC 2 cut(s) 641, 1145
TspDTI ATGAA 2 cut(s) 417, 1149
TspGWI ACGGA 1 cut(s) 666
TspRI CASTG 2 cut(s) 1201, 1288
VpaK11BI GGWCC 1 cut(s) 687
XapI RAATTY 3 cut(s) 495, 1088, 1101
XceI RCATGY 2 cut(s) 455, 671
XhoI CTCGAG 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 567
XmnI GAANNNNTTC 2 cut(s) 892, 978
XspI CTAG 3 cut(s) 62, 329, 843
Zsp2I ATGCAT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.