Rroxscaffold_4G00277580

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0006698
Physical Location & Seq
Forward (+)
954989 .. 958510
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277580.1

Sequence Viewer

Length: 1383 bp
ATGACGAAGATGATGAAGGGTAGGGTGTCCAGAATTGGAAGCTATGCAATTGCTTCTTCCATTAAAGATGGTCATCATCAACAGCCTTGCATTACCTGCACCACATTCAACATACTCGCTCCAATTTACAAGCGCCTAAGCCATGAGGACCCAGATTGTAGGGAAAGCGATCACAGAGCTTATTGGCTGGCCAGGAATCACATGATTTTGGATTGTTTGTTGGCTGAGAGATCTTCCATTATTTGTCTTCAGGAATTCTGGGTTGGGAATGAAGAGCTAGTGAACATTTATGAGAAGAGACTTGGTGATGCTGGTTATATCAATTTTAAGCTAGGGAGGACCAACAACCGCGGCGATGGTTTGCTGACCGCGGTGCATAAGGACTACTTTAGAGTTCTAAATTATCAAGAGTTGCACTTCCATGATTGTGGAGATCGTGTTGCTCAGTTGTTACATGTTGAATTAGCTTGCCCAATTTCACAGTTTCAAAACAATGATATTCGGCAGGAAATGCTTATTGTGAATACCCATTTGTTATTTCCTCATGATTCAAGCTTGTGTATTGTACGATTGCATCAGGTGTACAAAATCCTACAATATGTGGAATCTTATCAGAAAGAGAAAAAACTTAACCCCATGCCTATCATTCTCTGTGGTGACTGGAATGGAAGCAAACGTGGACATGTTTACAAGTTTCTTAGGTCCCAGGGGTTTGTATCTTCTTATGATACTGCTCATCATTACACCGATGCAGATGCACAAAAGTGGGTTAGCCACCGAAACCATCGGGGAAACATATGTGGGGTTGACTTTATATGGCTTCTCAACCCAAATAGTTACCGGAAACTGCTAAAAACAAGTTGGAGTGAAGCAGTATTTGGCATGTTCAAGTATCTACTACGAAGAGCTTCACTGACAGAAAGTGATGCCTTTGCCTTCCTGAAGGCTGACAACCCTGGTGATTACATTACCTATTCTGGCTTTTGTGAAGCCCTTCGACAGCTTAGTTTGACTGGTCATTGCCATGGACTTAGCCTTGAAGAGACCAAGGACTTGTGGGTTCAAGCAGACATAGATGGCAATGGTATTCTTGATTACAAAGGGTTTCAGCAGCGAATTTGGAATCCAACAGATGGTGGTAATGACATCTGGGATGTTGGCTCCAATGGTATTCAGGATCAAGCAATTGGTTTCAGTATAAAAAATGCAGTTCTTTTCCCTCCTGAAGCGGAGAAAGGAACATGGCCCGAAGACTACTCTCTTTCTGATCATGCACGACTCACTGTGGTTTTCTCACCGAACCTCCATCTTTCATACACCAGTGTTGACTACATCAGTGCCAACAAGCACATTAGCACAGGCCCTCCTATCTGCAGCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

460

Amino Acids

52.58

Weight (kDa)

6.61

Isoelectric Point (pI)

36.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 35 - 323 3.3e-08 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 104
AccB7I CCANNNNNTGG 1 cut(s) 1133
AccII CGCG 2 cut(s) 351, 371
AciI CCGC 5 cut(s) 349, 351, 369, 371, 1229
AclWI GGATC 1 cut(s) 1185
AcoI YGGCCR 1 cut(s) 189
AcsI RAATTY 2 cut(s) 254, 1116
AcuI CTGAAG 3 cut(s) 233, 962, 1245
AfaI GTAC 2 cut(s) 567, 584
AfiI CCNNNNNNNGG 1 cut(s) 1133
AflIII ACRYGT 2 cut(s) 454, 682
AgsI TTSAA 7 cut(s) 109, 461, 488, 552, 889, 1040, 1064
AjnI CCWGG 3 cut(s) 191, 705, 955
AjuI GAANNNNNNNTTGG 4 cut(s) 246, 278, 861, 893
AleI CACNNNNGTG 1 cut(s) 763
AluBI AGCT 9 cut(s) 42, 179, 277, 331, 467, 555, 908, 1003, 1377
AluI AGCT 9 cut(s) 42, 179, 277, 331, 467, 555, 908, 1003, 1377
Alw26I GTCTC 2 cut(s) 292, 1037
AlwI GGATC 1 cut(s) 1185
AoxI GGCC 3 cut(s) 189, 1244, 1360
ApeKI GCWGC 3 cut(s) 1111, 1374, 1377
ApoI RAATTY 2 cut(s) 254, 1116
Asp700I GAANNNNTTC 2 cut(s) 907, 993
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 5 cut(s) 148, 339, 702, 1245, 1361
AsuHPI GGTGA 4 cut(s) 317, 668, 971, 1287
AvaII GGWCC 3 cut(s) 148, 339, 702
BalI TGGCCA 1 cut(s) 191
BbsI GAAGAC 2 cut(s) 239, 1257
BbvI GCAGC 2 cut(s) 1123, 1364
BccI CCATC 6 cut(s) 62, 350, 792, 1070, 1127, 1314
BcgI CGANNNNNNTGC 2 cut(s) 737, 771
BciT130I CCWGG 3 cut(s) 193, 707, 957
BclI TGATCA 1 cut(s) 1267
BcoDI GTCTC 2 cut(s) 292, 1037
BfaI CTAG 2 cut(s) 278, 332
BfmI CTRYAG 1 cut(s) 1372
BfoI RGCGCY 1 cut(s) 136
BfuAI ACCTGC 1 cut(s) 104
BglII AGATCT 1 cut(s) 230
BisI GCNGC 4 cut(s) 352, 1112, 1375, 1378
BlsI GCNGC 4 cut(s) 353, 1113, 1376, 1379
Bme1390I CCNGG 3 cut(s) 193, 707, 957
Bme18I GGWCC 3 cut(s) 148, 339, 702
BmgT120I GGNCC 5 cut(s) 148, 339, 702, 1245, 1361
BmiI GGNNCC 3 cut(s) 150, 704, 1162
BmrFI CCNGG 3 cut(s) 193, 707, 957
BmsI GCATC 5 cut(s) 298, 583, 739, 745, 916
BpiI GAAGAC 2 cut(s) 239, 1257
Bpu10I CCTNAGC 1 cut(s) 137
BsaBI GATNNNNATC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 1037
BsaJI CCNNGG 7 cut(s) 349, 369, 705, 706, 955, 1024, 1047
BsaWI WCCGGW 1 cut(s) 840
BsaXI ACNNNNNCTCC 6 cut(s) 423, 453, 1287, 1317, 1348, 1378
Bsc4I CCNNNNNNNGG 1 cut(s) 1133
Bse1I ACTGG 3 cut(s) 665, 1018, 1320
Bse3DI GCAATG 2 cut(s) 1018, 1087
Bse8I GATNNNNATC 1 cut(s) 72
BseBI CCWGG 3 cut(s) 193, 707, 957
BseDI CCNNGG 7 cut(s) 349, 369, 705, 706, 955, 1024, 1047
BseGI GGATG 1 cut(s) 1159
BseJI GATNNNNATC 1 cut(s) 72
BseLI CCNNNNNNNGG 1 cut(s) 1133
BseMI GCAATG 2 cut(s) 1018, 1087
BseMII CTCAG 2 cut(s) 216, 458
BseNI ACTGG 3 cut(s) 665, 1018, 1320
BseXI GCAGC 2 cut(s) 1123, 1364
BsgI GTGCAG 1 cut(s) 82
Bsh1236I CGCG 2 cut(s) 351, 371
BshFI GGCC 3 cut(s) 191, 1246, 1362
BsiSI CCGG 1 cut(s) 841
BslFI GGGAC 1 cut(s) 688
BslI CCNNNNNNNGG 1 cut(s) 1133
BsmAI GTCTC 2 cut(s) 292, 1037
BsmFI GGGAC 1 cut(s) 688
BsnI GGCC 3 cut(s) 191, 1246, 1362
Bso31I GGTCTC 1 cut(s) 1037
Bsp1407I TGTACA 1 cut(s) 582
Bsp143I GATC 5 cut(s) 169, 230, 433, 1177, 1267
Bsp19I CCATGG 1 cut(s) 1024
BspACI CCGC 5 cut(s) 349, 351, 369, 371, 1229
BspANI GGCC 3 cut(s) 191, 1246, 1362
BspCNI CTCAG 2 cut(s) 217, 457
BspFNI CGCG 2 cut(s) 351, 371
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 3 cut(s) 150, 704, 1162
BspMAI CTGCAG 1 cut(s) 1376
BspMI ACCTGC 1 cut(s) 104
BspPI GGATC 1 cut(s) 1185
BspQI GCTCTTC 2 cut(s) 267, 898
BspTNI GGTCTC 1 cut(s) 1037
BsrDI GCAATG 2 cut(s) 1018, 1087
BsrGI TGTACA 1 cut(s) 582
BsrI ACTGG 3 cut(s) 665, 1018, 1320
BssECI CCNNGG 7 cut(s) 349, 369, 705, 706, 955, 1024, 1047
BssMI GATC 5 cut(s) 169, 230, 433, 1177, 1267
BssT1I CCWWGG 2 cut(s) 1024, 1047
Bst2UI CCWGG 3 cut(s) 193, 707, 957
Bst4CI ACNGT 2 cut(s) 483, 1285
Bst6I CTCTTC 4 cut(s) 267, 290, 898, 1035
BstAPI GCANNNNNTGC 2 cut(s) 96, 511
BstAUI TGTACA 1 cut(s) 582
BstC8I GCNNGC 2 cut(s) 189, 469
BstDEI CTNAG 6 cut(s) 137, 225, 444, 698, 1004, 1031
BstDSI CCRYGG 3 cut(s) 349, 369, 1024
BstF5I GGATG 1 cut(s) 1159
BstFNI CGCG 2 cut(s) 351, 371
BstH2I RGCGCY 1 cut(s) 136
BstHHI GCGC 1 cut(s) 135
BstKTI GATC 5 cut(s) 172, 233, 436, 1180, 1270
BstMAI GTCTC 2 cut(s) 292, 1037
BstMBI GATC 5 cut(s) 169, 230, 433, 1177, 1267
BstMWI GCNNNNNNNGC 2 cut(s) 96, 511
BstNI CCWGG 3 cut(s) 193, 707, 957
BstNSI RCATGY 3 cut(s) 458, 686, 886
BstSCI CCNGG 3 cut(s) 191, 705, 955
BstSFI CTRYAG 1 cut(s) 1372
BstUI CGCG 2 cut(s) 351, 371
BstV1I GCAGC 2 cut(s) 1123, 1364
BstV2I GAAGAC 2 cut(s) 239, 1257
BstX2I RGATCY 1 cut(s) 230
BstXI CCANNNNNNTGG 1 cut(s) 428
BstYI RGATCY 1 cut(s) 230
BsuRI GGCC 3 cut(s) 191, 1246, 1362
BtgI CCRYGG 3 cut(s) 349, 369, 1024
BtgZI GCGATG 1 cut(s) 369
BtsCI GGATG 1 cut(s) 1159
BtsIMutI CAGTG 4 cut(s) 911, 1281, 1327, 1342
BveI ACCTGC 1 cut(s) 104
Cac8I GCNNGC 2 cut(s) 189, 469
CciI TCATGA 1 cut(s) 544
CfoI GCGC 1 cut(s) 135
Cfr13I GGNCC 5 cut(s) 148, 339, 702, 1245, 1361
Cfr42I CCGCGG 2 cut(s) 352, 372
Csp6I GTAC 2 cut(s) 566, 583
CviQI GTAC 2 cut(s) 566, 583
DdeI CTNAG 6 cut(s) 137, 225, 444, 698, 1004, 1031
DpnI GATC 5 cut(s) 171, 232, 435, 1179, 1269
DpnII GATC 5 cut(s) 169, 230, 433, 1177, 1267
EaeI YGGCCR 1 cut(s) 189
Eam1104I CTCTTC 4 cut(s) 267, 290, 898, 1035
EarI CTCTTC 4 cut(s) 267, 290, 898, 1035
Eco130I CCWWGG 2 cut(s) 1024, 1047
Eco31I GGTCTC 1 cut(s) 1037
Eco47I GGWCC 3 cut(s) 148, 339, 702
Eco57I CTGAAG 3 cut(s) 233, 962, 1245
EcoO109I RGGNCCY 3 cut(s) 148, 702, 1361
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 3 cut(s) 191, 705, 955
EcoT14I CCWWGG 2 cut(s) 1024, 1047
ErhI CCWWGG 2 cut(s) 1024, 1047
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FaqI GGGAC 1 cut(s) 688
FauNDI CATATG 1 cut(s) 797
FbaI TGATCA 1 cut(s) 1267
Fnu4HI GCNGC 4 cut(s) 352, 1112, 1375, 1378
FokI GGATG 1 cut(s) 1166
Fsp4HI GCNGC 4 cut(s) 352, 1112, 1375, 1378
FspBI CTAG 2 cut(s) 278, 332
GlaI GCGC 1 cut(s) 134
GluI GCNGC 4 cut(s) 352, 1112, 1375, 1378
HaeII RGCGCY 1 cut(s) 136
HaeIII GGCC 3 cut(s) 191, 1246, 1362
HapII CCGG 1 cut(s) 841
HhaI GCGC 1 cut(s) 135
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HincII GTYRAC 2 cut(s) 808, 1327
HindII GTYRAC 2 cut(s) 808, 1327
HindIII AAGCTT 1 cut(s) 553
HinfI GANTC 5 cut(s) 196, 548, 605, 1123, 1278
HpaII CCGG 1 cut(s) 841
HphI GGTGA 4 cut(s) 317, 668, 971, 1287
Hpy166II GTNNAC 6 cut(s) 283, 583, 680, 688, 808, 1327
Hpy188I TCNGA 2 cut(s) 615, 1267
Hpy188III TCNNGA 8 cut(s) 30, 251, 407, 545, 940, 1091, 1175, 1223
Hpy8I GTNNAC 6 cut(s) 283, 583, 680, 688, 808, 1327
HpyAV CCTTC 4 cut(s) 10, 937, 946, 1004
HpyCH4III ACNGT 2 cut(s) 483, 1285
HpyCH4IV ACGT 1 cut(s) 676
HpyF10VI GCNNNNNNNGC 2 cut(s) 96, 511
HpyF3I CTNAG 6 cut(s) 137, 225, 444, 698, 1004, 1031
HpySE526I ACGT 1 cut(s) 676
HspAI GCGC 1 cut(s) 133
Ksp22I TGATCA 1 cut(s) 1267
KspI CCGCGG 2 cut(s) 352, 372
Kzo9I GATC 5 cut(s) 169, 230, 433, 1177, 1267
LguI GCTCTTC 2 cut(s) 267, 898
LmnI GCTCC 2 cut(s) 124, 1166
Lsp1109I GCAGC 2 cut(s) 1123, 1364
LweI GCATC 5 cut(s) 298, 583, 739, 745, 916
MaeI CTAG 2 cut(s) 278, 332
MaeII ACGT 1 cut(s) 676
MaeIII GTNAC 3 cut(s) 450, 656, 836
MalI GATC 5 cut(s) 171, 232, 435, 1179, 1269
MboI GATC 5 cut(s) 169, 230, 433, 1177, 1267
MfeI CAATTG 2 cut(s) 48, 1185
MflI RGATCY 1 cut(s) 230
MlsI TGGCCA 1 cut(s) 191
MluNI TGGCCA 1 cut(s) 191
MlyI GAGTC 1 cut(s) 1272
MmeI TCCRAC 2 cut(s) 842, 1151
MnlI CCTC 6 cut(s) 139, 330, 552, 1230, 1313, 1374
Mox20I TGGCCA 1 cut(s) 191
MroXI GAANNNNTTC 2 cut(s) 907, 993
MscI TGGCCA 1 cut(s) 191
MseI TTAA 3 cut(s) 63, 327, 630
MslI CAYNNNNRTG 4 cut(s) 420, 426, 763, 1023
Msp20I TGGCCA 1 cut(s) 191
MspA1I CMGCKG 3 cut(s) 351, 371, 1377
MspI CCGG 1 cut(s) 841
MspR9I CCNGG 3 cut(s) 193, 707, 957
MunI CAATTG 2 cut(s) 48, 1185
MvaI CCWGG 3 cut(s) 193, 707, 957
MvnI CGCG 2 cut(s) 351, 371
MwoI GCNNNNNNNGC 2 cut(s) 96, 511
NcoI CCATGG 1 cut(s) 1024
NdeI CATATG 1 cut(s) 797
NdeII GATC 5 cut(s) 169, 230, 433, 1177, 1267
NlaIV GGNNCC 3 cut(s) 150, 704, 1162
NmuCI GTSAC 1 cut(s) 656
NspI RCATGY 3 cut(s) 458, 686, 886
OliI CACNNNNGTG 1 cut(s) 763
PagI TCATGA 1 cut(s) 544
PasI CCCWGGG 1 cut(s) 706
PciI ACATGT 2 cut(s) 454, 682
PciSI GCTCTTC 2 cut(s) 267, 898
PdmI GAANNNNTTC 2 cut(s) 907, 993
PfeI GAWTC 4 cut(s) 196, 548, 605, 1123
PflMI CCANNNNNTGG 1 cut(s) 1133
PkrI GCNGC 4 cut(s) 353, 1113, 1376, 1379
PleI GAGTC 1 cut(s) 1272
PpsI GAGTC 1 cut(s) 1272
PpuMI RGGWCCY 2 cut(s) 148, 702
PscI ACATGT 2 cut(s) 454, 682
Psp5II RGGWCCY 2 cut(s) 148, 702
Psp6I CCWGG 3 cut(s) 191, 705, 955
PspGI CCWGG 3 cut(s) 191, 705, 955
PspN4I GGNNCC 3 cut(s) 150, 704, 1162
PspPI GGNCC 5 cut(s) 148, 339, 702, 1245, 1361
PspPPI RGGWCCY 2 cut(s) 148, 702
PstI CTGCAG 1 cut(s) 1376
PsuI RGATCY 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 1377
RsaI GTAC 2 cut(s) 567, 584
RsaNI GTAC 2 cut(s) 566, 583
RseI CAYNNNNRTG 4 cut(s) 420, 426, 763, 1023
SacII CCGCGG 2 cut(s) 352, 372
SapI GCTCTTC 2 cut(s) 267, 898
SaqAI TTAA 3 cut(s) 63, 327, 630
SatI GCNGC 4 cut(s) 352, 1112, 1375, 1378
Sau3AI GATC 5 cut(s) 169, 230, 433, 1177, 1267
Sau96I GGNCC 5 cut(s) 148, 339, 702, 1245, 1361
SchI GAGTC 1 cut(s) 1272
ScrFI CCNGG 3 cut(s) 193, 707, 957
SfaNI GCATC 5 cut(s) 298, 583, 739, 745, 916
SfcI CTRYAG 1 cut(s) 1372
Sfr303I CCGCGG 2 cut(s) 352, 372
SgrBI CCGCGG 2 cut(s) 352, 372
SinI GGWCC 3 cut(s) 148, 339, 702
SmiMI CAYNNNNRTG 4 cut(s) 420, 426, 763, 1023
SsiI CCGC 5 cut(s) 349, 351, 369, 371, 1229
SspMI CTAG 2 cut(s) 278, 332
StyD4I CCNGG 3 cut(s) 191, 705, 955
StyI CCWWGG 2 cut(s) 1024, 1047
TaaI ACNGT 2 cut(s) 483, 1285
TaiI ACGT 1 cut(s) 679
TaqI TCGA 1 cut(s) 997
TatI WGTACW 1 cut(s) 582
TauI GCSGC 1 cut(s) 354
TfiI GAWTC 4 cut(s) 196, 548, 605, 1123
Tru1I TTAA 3 cut(s) 63, 327, 630
Tru9I TTAA 3 cut(s) 63, 327, 630
TscAI CASTG 4 cut(s) 918, 1288, 1327, 1342
TseFI GTSAC 1 cut(s) 656
TseI GCWGC 3 cut(s) 1111, 1374, 1377
Tsp45I GTSAC 1 cut(s) 656
TspDTI ATGAA 3 cut(s) 29, 285, 1302
TspRI CASTG 4 cut(s) 918, 1288, 1327, 1342
Van91I CCANNNNNTGG 1 cut(s) 1133
VpaK11BI GGWCC 3 cut(s) 148, 339, 702
XapI RAATTY 2 cut(s) 254, 1116
XceI RCATGY 3 cut(s) 458, 686, 886
XmnI GAANNNNTTC 2 cut(s) 907, 993
XspI CTAG 2 cut(s) 278, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.